Journal: Molecular Neurobiology
Article Title: Involvement of the Voltage-Gated Calcium Channels L- P/Q- and N-Types in Synapse Elimination During Neuromuscular Junction Development
Figure Lengend Snippet: Graphic representation of the results. The activity-dependent signaling between the nerve terminals that are in competition through several metabotropic receptors can result in the modulation of the downstream effector kinases, specifically cPKCβI, nPKCε, and PKA. Changes in kinases activity can allow the coordinate phosphorylation of the L-type CaV1.3 and P/Q-type VGCC. The high calcium entry through these operative channels present in immature nerve endings can result in their final loss. Also, muscle CaV1.1 and contractile activity can contribute to the synapse elimination. A component of this mechanism may be mediated by a retrograde influence from the postsynaptic site, via the BDNF-TrkB pathway, on the presynaptic calcium channels
Article Snippet: Primary antibodies were incubated at 4 °C overnight (rabbit anti-P/Q-type calcium channel (1:1000; ACC-001, Alomone; Jerusalem, Israel); rabbit anti-α1D L-type calcium channel (CaV1.3, 1:500; ACC-005, Alomone, Jerusalem, Israel); rabbit anti-N-type calcium channel (1:500; ACC-002, Alomone); rabbit anti-Munc18-1 (1:1000; ≠ 13414, Cell Signalling Technology; Massachusetts, USA), and rabbit anti-PKCε (1:1000; ≠ 2683, Cell Signalling Technology; Massachusetts, USA)).
Techniques: Activity Assay