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NeuroMab anti cav1 2 calcium channel
Myoscape expression profile and subcellular localization. ( a ) Mouse muscle and heart Myoscape mRNA expression profile confirmed by HPRT1-normalized quantitative qPCR and ( b ) additional Northern blot analyses of multiple human tissues using Myoscape-specific probes and primers. Confirmation of the interaction between α-actinin 2 ( c ) and the skeletal muscle-specific pore-forming unit ( d ) of the LTCC (CACNA 1S) with myc-tagged Myoscape by co-immunoprecipitation of the proteins in HEK-T cells. ( e ) Immunoprecipitation performed with endogenous proteins isolated from mouse heart using anti-V5 (control) or <t>anti-Cav1.2</t> antibody followed by immunoblotting performed with Myoscape antibody, indicates the physiological interaction between Cav1.2 and Myoscape. ( f – g ) Confocal Immunofluorescence analysis of Myoscape expression in isolated adult rat ventricular myocytes shows a striated signal. Myoscape is localized to couplons near the sarcomeric z-band/t-tubule interface, as evidenced by coimmunostaining of Myoscape and actinin 2 and LTCC and RYR2. Scale bars, 20 μm.
Anti Cav1 2 Calcium Channel, supplied by NeuroMab, used in various techniques. Bioz Stars score: 86/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti cav1 2 calcium channel/product/NeuroMab
Average 86 stars, based on 3 article reviews
Price from $9.99 to $1999.99
anti cav1 2 calcium channel - by Bioz Stars, 2022-11
86/100 stars

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1) Product Images from "Myoscape controls cardiac calcium cycling and contractility via regulation of L-type calcium channel surface expression"

Article Title: Myoscape controls cardiac calcium cycling and contractility via regulation of L-type calcium channel surface expression

Journal: Nature Communications

doi: 10.1038/ncomms11317

Myoscape expression profile and subcellular localization. ( a ) Mouse muscle and heart Myoscape mRNA expression profile confirmed by HPRT1-normalized quantitative qPCR and ( b ) additional Northern blot analyses of multiple human tissues using Myoscape-specific probes and primers. Confirmation of the interaction between α-actinin 2 ( c ) and the skeletal muscle-specific pore-forming unit ( d ) of the LTCC (CACNA 1S) with myc-tagged Myoscape by co-immunoprecipitation of the proteins in HEK-T cells. ( e ) Immunoprecipitation performed with endogenous proteins isolated from mouse heart using anti-V5 (control) or anti-Cav1.2 antibody followed by immunoblotting performed with Myoscape antibody, indicates the physiological interaction between Cav1.2 and Myoscape. ( f – g ) Confocal Immunofluorescence analysis of Myoscape expression in isolated adult rat ventricular myocytes shows a striated signal. Myoscape is localized to couplons near the sarcomeric z-band/t-tubule interface, as evidenced by coimmunostaining of Myoscape and actinin 2 and LTCC and RYR2. Scale bars, 20 μm.
Figure Legend Snippet: Myoscape expression profile and subcellular localization. ( a ) Mouse muscle and heart Myoscape mRNA expression profile confirmed by HPRT1-normalized quantitative qPCR and ( b ) additional Northern blot analyses of multiple human tissues using Myoscape-specific probes and primers. Confirmation of the interaction between α-actinin 2 ( c ) and the skeletal muscle-specific pore-forming unit ( d ) of the LTCC (CACNA 1S) with myc-tagged Myoscape by co-immunoprecipitation of the proteins in HEK-T cells. ( e ) Immunoprecipitation performed with endogenous proteins isolated from mouse heart using anti-V5 (control) or anti-Cav1.2 antibody followed by immunoblotting performed with Myoscape antibody, indicates the physiological interaction between Cav1.2 and Myoscape. ( f – g ) Confocal Immunofluorescence analysis of Myoscape expression in isolated adult rat ventricular myocytes shows a striated signal. Myoscape is localized to couplons near the sarcomeric z-band/t-tubule interface, as evidenced by coimmunostaining of Myoscape and actinin 2 and LTCC and RYR2. Scale bars, 20 μm.

Techniques Used: Expressing, Real-time Polymerase Chain Reaction, Northern Blot, Immunoprecipitation, Isolation, Immunofluorescence

2) Product Images from "Myoscape controls cardiac calcium cycling and contractility via regulation of L-type calcium channel surface expression"

Article Title: Myoscape controls cardiac calcium cycling and contractility via regulation of L-type calcium channel surface expression

Journal: Nature Communications

doi: 10.1038/ncomms11317

Myoscape expression profile and subcellular localization. ( a ) Mouse muscle and heart Myoscape mRNA expression profile confirmed by HPRT1-normalized quantitative qPCR and ( b ) additional Northern blot analyses of multiple human tissues using Myoscape-specific probes and primers. Confirmation of the interaction between α-actinin 2 ( c ) and the skeletal muscle-specific pore-forming unit ( d ) of the LTCC (CACNA 1S) with myc-tagged Myoscape by co-immunoprecipitation of the proteins in HEK-T cells. ( e ) Immunoprecipitation performed with endogenous proteins isolated from mouse heart using anti-V5 (control) or anti-Cav1.2 antibody followed by immunoblotting performed with Myoscape antibody, indicates the physiological interaction between Cav1.2 and Myoscape. ( f – g ) Confocal Immunofluorescence analysis of Myoscape expression in isolated adult rat ventricular myocytes shows a striated signal. Myoscape is localized to couplons near the sarcomeric z-band/t-tubule interface, as evidenced by coimmunostaining of Myoscape and actinin 2 and LTCC and RYR2. Scale bars, 20 μm.
Figure Legend Snippet: Myoscape expression profile and subcellular localization. ( a ) Mouse muscle and heart Myoscape mRNA expression profile confirmed by HPRT1-normalized quantitative qPCR and ( b ) additional Northern blot analyses of multiple human tissues using Myoscape-specific probes and primers. Confirmation of the interaction between α-actinin 2 ( c ) and the skeletal muscle-specific pore-forming unit ( d ) of the LTCC (CACNA 1S) with myc-tagged Myoscape by co-immunoprecipitation of the proteins in HEK-T cells. ( e ) Immunoprecipitation performed with endogenous proteins isolated from mouse heart using anti-V5 (control) or anti-Cav1.2 antibody followed by immunoblotting performed with Myoscape antibody, indicates the physiological interaction between Cav1.2 and Myoscape. ( f – g ) Confocal Immunofluorescence analysis of Myoscape expression in isolated adult rat ventricular myocytes shows a striated signal. Myoscape is localized to couplons near the sarcomeric z-band/t-tubule interface, as evidenced by coimmunostaining of Myoscape and actinin 2 and LTCC and RYR2. Scale bars, 20 μm.

Techniques Used: Expressing, Real-time Polymerase Chain Reaction, Northern Blot, Immunoprecipitation, Isolation, Immunofluorescence

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    NeuroMab anti cav1 2 calcium channel
    Myoscape expression profile and subcellular localization. ( a ) Mouse muscle and heart Myoscape mRNA expression profile confirmed by HPRT1-normalized quantitative qPCR and ( b ) additional Northern blot analyses of multiple human tissues using Myoscape-specific probes and primers. Confirmation of the interaction between α-actinin 2 ( c ) and the skeletal muscle-specific pore-forming unit ( d ) of the LTCC (CACNA 1S) with myc-tagged Myoscape by co-immunoprecipitation of the proteins in HEK-T cells. ( e ) Immunoprecipitation performed with endogenous proteins isolated from mouse heart using anti-V5 (control) or <t>anti-Cav1.2</t> antibody followed by immunoblotting performed with Myoscape antibody, indicates the physiological interaction between Cav1.2 and Myoscape. ( f – g ) Confocal Immunofluorescence analysis of Myoscape expression in isolated adult rat ventricular myocytes shows a striated signal. Myoscape is localized to couplons near the sarcomeric z-band/t-tubule interface, as evidenced by coimmunostaining of Myoscape and actinin 2 and LTCC and RYR2. Scale bars, 20 μm.
    Anti Cav1 2 Calcium Channel, supplied by NeuroMab, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti cav1 2 calcium channel/product/NeuroMab
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti cav1 2 calcium channel - by Bioz Stars, 2022-11
    86/100 stars
      Buy from Supplier

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    Myoscape expression profile and subcellular localization. ( a ) Mouse muscle and heart Myoscape mRNA expression profile confirmed by HPRT1-normalized quantitative qPCR and ( b ) additional Northern blot analyses of multiple human tissues using Myoscape-specific probes and primers. Confirmation of the interaction between α-actinin 2 ( c ) and the skeletal muscle-specific pore-forming unit ( d ) of the LTCC (CACNA 1S) with myc-tagged Myoscape by co-immunoprecipitation of the proteins in HEK-T cells. ( e ) Immunoprecipitation performed with endogenous proteins isolated from mouse heart using anti-V5 (control) or anti-Cav1.2 antibody followed by immunoblotting performed with Myoscape antibody, indicates the physiological interaction between Cav1.2 and Myoscape. ( f – g ) Confocal Immunofluorescence analysis of Myoscape expression in isolated adult rat ventricular myocytes shows a striated signal. Myoscape is localized to couplons near the sarcomeric z-band/t-tubule interface, as evidenced by coimmunostaining of Myoscape and actinin 2 and LTCC and RYR2. Scale bars, 20 μm.

    Journal: Nature Communications

    Article Title: Myoscape controls cardiac calcium cycling and contractility via regulation of L-type calcium channel surface expression

    doi: 10.1038/ncomms11317

    Figure Lengend Snippet: Myoscape expression profile and subcellular localization. ( a ) Mouse muscle and heart Myoscape mRNA expression profile confirmed by HPRT1-normalized quantitative qPCR and ( b ) additional Northern blot analyses of multiple human tissues using Myoscape-specific probes and primers. Confirmation of the interaction between α-actinin 2 ( c ) and the skeletal muscle-specific pore-forming unit ( d ) of the LTCC (CACNA 1S) with myc-tagged Myoscape by co-immunoprecipitation of the proteins in HEK-T cells. ( e ) Immunoprecipitation performed with endogenous proteins isolated from mouse heart using anti-V5 (control) or anti-Cav1.2 antibody followed by immunoblotting performed with Myoscape antibody, indicates the physiological interaction between Cav1.2 and Myoscape. ( f – g ) Confocal Immunofluorescence analysis of Myoscape expression in isolated adult rat ventricular myocytes shows a striated signal. Myoscape is localized to couplons near the sarcomeric z-band/t-tubule interface, as evidenced by coimmunostaining of Myoscape and actinin 2 and LTCC and RYR2. Scale bars, 20 μm.

    Article Snippet: For mouse LTCC western blots an Anti-Cav1.2 calcium channel, NeuroMab clone L57/46 antibody was used (1:200).

    Techniques: Expressing, Real-time Polymerase Chain Reaction, Northern Blot, Immunoprecipitation, Isolation, Immunofluorescence