rabbit anti cav1 2  (Alomone Labs)


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    Structured Review

    Alomone Labs rabbit anti cav1 2
    GJA1-20k is upregulated with acute cardiac I/R injury. ( A ) Schematic of the protocol used for I/R injury in Langendorff-perfused mouse hearts. After a 20-minute stabilization period, mouse hearts were subjected to continuous perfusion for 90 minutes as a control, or subjected to 30 minutes of ischemia followed by 60 minutes of reperfusion for I/R injury. ( B ) Western blot of heart tissue lysates after either I/R injury or control perfusion. The blots are probed with an antibody for Cx43 C-terminus and an anti-actin antibody. ( C ) Protein expression for GJA1-20k is normalized to actin and shown as fold change after I/R injury in the quantified graphs. ( D ) Western blot showing <t>Cav1.2</t> protein levels in the control perfused hearts and after I/R injury. ( E ) Cav1.2 expression is normalized to actin and shown as fold change after I/R injury in the quantified graph. The number of hearts examined in C and E is shown on the graphs, and data are presented as mean fold change ± SEM. ** P
    Rabbit Anti Cav1 2, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 95/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti cav1 2/product/Alomone Labs
    Average 95 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    rabbit anti cav1 2 - by Bioz Stars, 2022-09
    95/100 stars

    Images

    1) Product Images from "Stress response protein GJA1-20k promotes mitochondrial biogenesis, metabolic quiescence, and cardioprotection against ischemia/reperfusion injury"

    Article Title: Stress response protein GJA1-20k promotes mitochondrial biogenesis, metabolic quiescence, and cardioprotection against ischemia/reperfusion injury

    Journal: JCI Insight

    doi: 10.1172/jci.insight.121900

    GJA1-20k is upregulated with acute cardiac I/R injury. ( A ) Schematic of the protocol used for I/R injury in Langendorff-perfused mouse hearts. After a 20-minute stabilization period, mouse hearts were subjected to continuous perfusion for 90 minutes as a control, or subjected to 30 minutes of ischemia followed by 60 minutes of reperfusion for I/R injury. ( B ) Western blot of heart tissue lysates after either I/R injury or control perfusion. The blots are probed with an antibody for Cx43 C-terminus and an anti-actin antibody. ( C ) Protein expression for GJA1-20k is normalized to actin and shown as fold change after I/R injury in the quantified graphs. ( D ) Western blot showing Cav1.2 protein levels in the control perfused hearts and after I/R injury. ( E ) Cav1.2 expression is normalized to actin and shown as fold change after I/R injury in the quantified graph. The number of hearts examined in C and E is shown on the graphs, and data are presented as mean fold change ± SEM. ** P
    Figure Legend Snippet: GJA1-20k is upregulated with acute cardiac I/R injury. ( A ) Schematic of the protocol used for I/R injury in Langendorff-perfused mouse hearts. After a 20-minute stabilization period, mouse hearts were subjected to continuous perfusion for 90 minutes as a control, or subjected to 30 minutes of ischemia followed by 60 minutes of reperfusion for I/R injury. ( B ) Western blot of heart tissue lysates after either I/R injury or control perfusion. The blots are probed with an antibody for Cx43 C-terminus and an anti-actin antibody. ( C ) Protein expression for GJA1-20k is normalized to actin and shown as fold change after I/R injury in the quantified graphs. ( D ) Western blot showing Cav1.2 protein levels in the control perfused hearts and after I/R injury. ( E ) Cav1.2 expression is normalized to actin and shown as fold change after I/R injury in the quantified graph. The number of hearts examined in C and E is shown on the graphs, and data are presented as mean fold change ± SEM. ** P

    Techniques Used: Western Blot, Expressing

    2) Product Images from "Atrial Fibrillation in Heart Failure Is Associated with High Levels of Circulating microRNA-199a-5p and 22–5p and a Defective Regulation of Intracellular Calcium and Cell-to-Cell Communication"

    Article Title: Atrial Fibrillation in Heart Failure Is Associated with High Levels of Circulating microRNA-199a-5p and 22–5p and a Defective Regulation of Intracellular Calcium and Cell-to-Cell Communication

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms221910377

    Gene and protein expression ( A – C ). Expression levels of CACNA1C , SLC8A1 and the connexin members, GJA5 , GJA1 and GJC1 in HL-1 cells transfected with miR-NC (black columns) or miR-199a+miR-22 (white columns). Normalized to miR-NC ( D – F ). Protein expression levels of Cav1.2, NCX1 and Cx40, normalized to the endogenous control, tubulin ( G ). Representative WB experiments in HL-1 cells transfected with miR-22+199a and miR-NC. All proteins (Cav1.2, NCX1, connexin 40 and tubulin) were blotted on the same gel. This is a representative image of four independent experiments. Numbers within columns represent the number of independent experiments done. * p
    Figure Legend Snippet: Gene and protein expression ( A – C ). Expression levels of CACNA1C , SLC8A1 and the connexin members, GJA5 , GJA1 and GJC1 in HL-1 cells transfected with miR-NC (black columns) or miR-199a+miR-22 (white columns). Normalized to miR-NC ( D – F ). Protein expression levels of Cav1.2, NCX1 and Cx40, normalized to the endogenous control, tubulin ( G ). Representative WB experiments in HL-1 cells transfected with miR-22+199a and miR-NC. All proteins (Cav1.2, NCX1, connexin 40 and tubulin) were blotted on the same gel. This is a representative image of four independent experiments. Numbers within columns represent the number of independent experiments done. * p

    Techniques Used: Expressing, Transfection, Western Blot

    3) Product Images from "Attenuated β-adrenergic response in calcium/calmodulin-dependent protein kinase IV-knockout mice"

    Article Title: Attenuated β-adrenergic response in calcium/calmodulin-dependent protein kinase IV-knockout mice

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0249932

    A) Typical RT-PCR data from wild-type (left; WT) and CaMKIV-null (right; CaMKIVko) mice. The amplified sequences are shown. Identification of sympathetic β-adrenergic receptor (β1)-specific transcripts in the heart. CREB1, cholinergic M2 receptor, α-MHC, β-MHC, MEF, GATA4, BNP, ANF, and VDCC transcripts in the heart were also evaluated. Expression of β-actin was evaluated as a control. The primer sets used for PCR amplification are shown. At least four independent RT-PCR outputs were examined for each expression analysis. B) Whole-cell fluorescence immunohistochemistry of CaV1.2 in isolated cardiac myocytes from wild-type and CaMKIV-null mice. Scale bar = 50 μm. CaMKIV-null myocytes showed significantly decreased CaV1.2 levels. C) Western blot analysis of the hearts from wild-type and CaMKIV-null mice. Representative immunoblots of membranes from wild-type and transgenic mice analyzed for the expression of CaV1.2 (α1C) and GAPDH. A significant decrease in the Cav1.2 protein level was observed. n = 6. D) Whole hearts from wild-type (left) and CaMKIV-null (right) mice. E) Histological analysis of hearts from 12-week-old wild-type and CaMKIV-null mice. Hearts were sectioned transversely and stained with hematoxylin and eosin.
    Figure Legend Snippet: A) Typical RT-PCR data from wild-type (left; WT) and CaMKIV-null (right; CaMKIVko) mice. The amplified sequences are shown. Identification of sympathetic β-adrenergic receptor (β1)-specific transcripts in the heart. CREB1, cholinergic M2 receptor, α-MHC, β-MHC, MEF, GATA4, BNP, ANF, and VDCC transcripts in the heart were also evaluated. Expression of β-actin was evaluated as a control. The primer sets used for PCR amplification are shown. At least four independent RT-PCR outputs were examined for each expression analysis. B) Whole-cell fluorescence immunohistochemistry of CaV1.2 in isolated cardiac myocytes from wild-type and CaMKIV-null mice. Scale bar = 50 μm. CaMKIV-null myocytes showed significantly decreased CaV1.2 levels. C) Western blot analysis of the hearts from wild-type and CaMKIV-null mice. Representative immunoblots of membranes from wild-type and transgenic mice analyzed for the expression of CaV1.2 (α1C) and GAPDH. A significant decrease in the Cav1.2 protein level was observed. n = 6. D) Whole hearts from wild-type (left) and CaMKIV-null (right) mice. E) Histological analysis of hearts from 12-week-old wild-type and CaMKIV-null mice. Hearts were sectioned transversely and stained with hematoxylin and eosin.

    Techniques Used: Reverse Transcription Polymerase Chain Reaction, Mouse Assay, Amplification, Expressing, Polymerase Chain Reaction, Fluorescence, Immunohistochemistry, Isolation, Western Blot, Transgenic Assay, Staining

    4) Product Images from "The impact of age and frailty on ventricular structure and function in C57BL/6J mice"

    Article Title: The impact of age and frailty on ventricular structure and function in C57BL/6J mice

    Journal: The Journal of Physiology

    doi: 10.1113/JP274134

    Frailty grades the age‐dependent decline in expression of Cav1.2 protein in the mouse heart A , representative Western blots for Cav1.2 protein expression in mice with varying FIs. Ponceau S staining was used as a loading control in all experiments (lower panels). B , mean expression of Cav1.2 decreased with age in the mouse heart. C , cardiac Cav1.2 expression was closely graded by frailty score in the mouse. Differences between age groups were assessed using a Mann–Whitney Rank Sum test and correlations were evaluated with linear regression analysis ( n = 8 adult and 8 aged hearts). Filled symbols indicate adult mice and open symbols indicate aged mice.
    Figure Legend Snippet: Frailty grades the age‐dependent decline in expression of Cav1.2 protein in the mouse heart A , representative Western blots for Cav1.2 protein expression in mice with varying FIs. Ponceau S staining was used as a loading control in all experiments (lower panels). B , mean expression of Cav1.2 decreased with age in the mouse heart. C , cardiac Cav1.2 expression was closely graded by frailty score in the mouse. Differences between age groups were assessed using a Mann–Whitney Rank Sum test and correlations were evaluated with linear regression analysis ( n = 8 adult and 8 aged hearts). Filled symbols indicate adult mice and open symbols indicate aged mice.

    Techniques Used: Expressing, Western Blot, Mouse Assay, Staining, MANN-WHITNEY

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    Alomone Labs cav1 2
    <t>Cav1.2</t> knock-down prevents astrocyte activation after scratch
    Cav1 2, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 95/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cav1 2/product/Alomone Labs
    Average 95 stars, based on 10 article reviews
    Price from $9.99 to $1999.99
    cav1 2 - by Bioz Stars, 2022-09
    95/100 stars
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    Image Search Results


    Cav1.2 knock-down prevents astrocyte activation after scratch

    Journal: Glia

    Article Title: L-type voltage-operated calcium channels contribute to astrocyte activation in vitro

    doi: 10.1002/glia.23013

    Figure Lengend Snippet: Cav1.2 knock-down prevents astrocyte activation after scratch

    Article Snippet: In contrast, RT-PCR, western blot and immunocytochemistry experiments show that Cav1.2 and Cav1.3 are highly expressed in cortical astrocytes ( ). shows representative images of labeled cultured astrocytes at 6 days in vitro ( DIV ); while GFAP abundantly labeled cell processes and cell bodies, labeling with Cav1.2 and Cav1.3 antibodies displayed a punctate staining on the cells surface ( ).

    Techniques: Activation Assay

    LPS enhance the activity and the expression of Cav1.2 Ca ++ channels in astrocytes

    Journal: Glia

    Article Title: L-type voltage-operated calcium channels contribute to astrocyte activation in vitro

    doi: 10.1002/glia.23013

    Figure Lengend Snippet: LPS enhance the activity and the expression of Cav1.2 Ca ++ channels in astrocytes

    Article Snippet: In contrast, RT-PCR, western blot and immunocytochemistry experiments show that Cav1.2 and Cav1.3 are highly expressed in cortical astrocytes ( ). shows representative images of labeled cultured astrocytes at 6 days in vitro ( DIV ); while GFAP abundantly labeled cell processes and cell bodies, labeling with Cav1.2 and Cav1.3 antibodies displayed a punctate staining on the cells surface ( ).

    Techniques: Activity Assay, Expressing

    Cav1.2 KO astrocytes are not sensitive to LPS

    Journal: Glia

    Article Title: L-type voltage-operated calcium channels contribute to astrocyte activation in vitro

    doi: 10.1002/glia.23013

    Figure Lengend Snippet: Cav1.2 KO astrocytes are not sensitive to LPS

    Article Snippet: In contrast, RT-PCR, western blot and immunocytochemistry experiments show that Cav1.2 and Cav1.3 are highly expressed in cortical astrocytes ( ). shows representative images of labeled cultured astrocytes at 6 days in vitro ( DIV ); while GFAP abundantly labeled cell processes and cell bodies, labeling with Cav1.2 and Cav1.3 antibodies displayed a punctate staining on the cells surface ( ).

    Techniques:

    Cav1.2 knock-down prevents astrocyte activation by LPS

    Journal: Glia

    Article Title: L-type voltage-operated calcium channels contribute to astrocyte activation in vitro

    doi: 10.1002/glia.23013

    Figure Lengend Snippet: Cav1.2 knock-down prevents astrocyte activation by LPS

    Article Snippet: In contrast, RT-PCR, western blot and immunocytochemistry experiments show that Cav1.2 and Cav1.3 are highly expressed in cortical astrocytes ( ). shows representative images of labeled cultured astrocytes at 6 days in vitro ( DIV ); while GFAP abundantly labeled cell processes and cell bodies, labeling with Cav1.2 and Cav1.3 antibodies displayed a punctate staining on the cells surface ( ).

    Techniques: Activation Assay

    Expressions of calcium handling proteins. The confocal image showed fluorescein isothiocyanate (green)-labeled Cav1.2, SERCA2a, and NCX1 expressed in isolated rat cardiac ventricular myocytes in SO (left panel), HF (middle panel), and NRG group (right panel). Nuclei were stained with DAPI (blue). The scale bar represents 20 μm (n = 5 for each set of staining).

    Journal: Frontiers in Pharmacology

    Article Title: Neuregulin-1β Partially Improves Cardiac Function in Volume-Overload Heart Failure Through Regulation of Abnormal Calcium Handling

    doi: 10.3389/fphar.2019.00616

    Figure Lengend Snippet: Expressions of calcium handling proteins. The confocal image showed fluorescein isothiocyanate (green)-labeled Cav1.2, SERCA2a, and NCX1 expressed in isolated rat cardiac ventricular myocytes in SO (left panel), HF (middle panel), and NRG group (right panel). Nuclei were stained with DAPI (blue). The scale bar represents 20 μm (n = 5 for each set of staining).

    Article Snippet: Anti-Cav1.2, anti-SERCA2a, and anti-NCX1 antibody were purchased from Alomone (Jerusalem, ACC-003, Israel), Millipore (MA, AB3516P, USA), and Santa Cruz Biotechnology (CA, SC-8094, USA), and secondary antibodies, anti-rabbit488 conjugated antibody was purchased from Santa Cruz Biotechnology (CA, sc-362262, USA).

    Techniques: Labeling, Isolation, Staining

    Quantitative analysis of calcium handling proteins expressions. (A) Representative expression of Cav1.2, SERCA2a, and NCX1, β-actin as loading controls. (B) Comparison of Cav1.2 protein expression (n = 3 rats). (C) Comparison of SERCA2a protein expression (n = 4 rats). (D) Comparison of NCX1 protein expression (n = 4 rats). * P

    Journal: Frontiers in Pharmacology

    Article Title: Neuregulin-1β Partially Improves Cardiac Function in Volume-Overload Heart Failure Through Regulation of Abnormal Calcium Handling

    doi: 10.3389/fphar.2019.00616

    Figure Lengend Snippet: Quantitative analysis of calcium handling proteins expressions. (A) Representative expression of Cav1.2, SERCA2a, and NCX1, β-actin as loading controls. (B) Comparison of Cav1.2 protein expression (n = 3 rats). (C) Comparison of SERCA2a protein expression (n = 4 rats). (D) Comparison of NCX1 protein expression (n = 4 rats). * P

    Article Snippet: Anti-Cav1.2, anti-SERCA2a, and anti-NCX1 antibody were purchased from Alomone (Jerusalem, ACC-003, Israel), Millipore (MA, AB3516P, USA), and Santa Cruz Biotechnology (CA, SC-8094, USA), and secondary antibodies, anti-rabbit488 conjugated antibody was purchased from Santa Cruz Biotechnology (CA, sc-362262, USA).

    Techniques: Expressing

    Location of antibody epitopes. Shown is a schematic of the Ca v 1.2 α 1 1.2 subunit, in which regions used as immunogens for the depicted antibodies are identified by arrows. Exact residues are listed in the table and numbered according to α 1 1.2 given in Gene Bank Accession number CAA33546. FP1, CNC1, and ACC-003 are directed against the loop between domains II and III, pS1700 against phosphorylated S1700, pS1928 against phosphorylated S1928, and CNC2 against residues 2122-2138 of α 1 1.2, which are ~40 residues upstream of the very C terminus of α 1 1.2.

    Journal: F1000Research

    Article Title: Proteolytic processing of the L-type Ca2+ channel alpha11.2 subunit in neurons

    doi: 10.12688/f1000research.11808.1

    Figure Lengend Snippet: Location of antibody epitopes. Shown is a schematic of the Ca v 1.2 α 1 1.2 subunit, in which regions used as immunogens for the depicted antibodies are identified by arrows. Exact residues are listed in the table and numbered according to α 1 1.2 given in Gene Bank Accession number CAA33546. FP1, CNC1, and ACC-003 are directed against the loop between domains II and III, pS1700 against phosphorylated S1700, pS1928 against phosphorylated S1928, and CNC2 against residues 2122-2138 of α 1 1.2, which are ~40 residues upstream of the very C terminus of α 1 1.2.

    Article Snippet: Concordant with this idea, neither the ACC-003 antibody that we received from Alomone Labs that recognized a 130 kDa band in WT and cKO brains nor our CNC1 antibody recognized the strong 150 kDa band seen with FP1 in brain lysate.

    Techniques:

    Differential recognition of the strong 150 kDa FP1 band in lysate and weak 150 kDa band by FP1, CNC1, and ACC-003 after IP of α 1 1.2 with FP1. Immunoblots with CNC1 ( A , B ), FP1 ( C ), and ACC-003 ( D , E ) of Triton X-100 extracts from WT mice (lysate) and after immunoprecipitation with FP1 from cKO and WT mice. Gels were polymerized from 8% acrylamide. Note that a weak 150 kDa band is detected by CNC1, FP1, and ACC-003 after enrichment of α 1 1.2 by immunoprecipitation with FP1 but the strongly immunoreactive 150 kDa band detected by FP1 in lysate is not detectable by either CNC1 or ACC-003.

    Journal: F1000Research

    Article Title: Proteolytic processing of the L-type Ca2+ channel alpha11.2 subunit in neurons

    doi: 10.12688/f1000research.11808.1

    Figure Lengend Snippet: Differential recognition of the strong 150 kDa FP1 band in lysate and weak 150 kDa band by FP1, CNC1, and ACC-003 after IP of α 1 1.2 with FP1. Immunoblots with CNC1 ( A , B ), FP1 ( C ), and ACC-003 ( D , E ) of Triton X-100 extracts from WT mice (lysate) and after immunoprecipitation with FP1 from cKO and WT mice. Gels were polymerized from 8% acrylamide. Note that a weak 150 kDa band is detected by CNC1, FP1, and ACC-003 after enrichment of α 1 1.2 by immunoprecipitation with FP1 but the strongly immunoreactive 150 kDa band detected by FP1 in lysate is not detectable by either CNC1 or ACC-003.

    Article Snippet: Concordant with this idea, neither the ACC-003 antibody that we received from Alomone Labs that recognized a 130 kDa band in WT and cKO brains nor our CNC1 antibody recognized the strong 150 kDa band seen with FP1 in brain lysate.

    Techniques: Western Blot, Mouse Assay, Immunoprecipitation

    Effect of CAL on I Ca , LTCC expression and the systolic Ca transient. A: Representative L-type Ca currents (I Ca ) recorded at 0 mV from Sham (left) and CAL (right) myocytes. B: Mean (± SEM) I Ca density-voltage relations from Sham (circles, n = 50/15) and CAL (squares, n = 41/15) myocytes. C: Representative Western blots of Sham (S) and CAL lysates probed with antibodies against LTCC (top) or GAPDH (bottom). D: Representative whole-cell Ca transients from Sham (grey line) and CAL (black line) myocytes during field stimulation (left); the right panel shows the early rise on an expanded time scale. E: Mean (± SEM) peak ΔF/F 0 , time to peak (TTP), and rate of rise (ΔF/F 0 .ms − 1 ) of the Ca transient in Sham (open bars, n = 10) and CAL (filled bars, n = 16) myocytes. ⁎⁎⁎ p

    Journal: Journal of Molecular and Cellular Cardiology

    Article Title: Altered distribution of ICa impairs Ca release at the t-tubules of ventricular myocytes from failing hearts

    doi: 10.1016/j.yjmcc.2015.06.012

    Figure Lengend Snippet: Effect of CAL on I Ca , LTCC expression and the systolic Ca transient. A: Representative L-type Ca currents (I Ca ) recorded at 0 mV from Sham (left) and CAL (right) myocytes. B: Mean (± SEM) I Ca density-voltage relations from Sham (circles, n = 50/15) and CAL (squares, n = 41/15) myocytes. C: Representative Western blots of Sham (S) and CAL lysates probed with antibodies against LTCC (top) or GAPDH (bottom). D: Representative whole-cell Ca transients from Sham (grey line) and CAL (black line) myocytes during field stimulation (left); the right panel shows the early rise on an expanded time scale. E: Mean (± SEM) peak ΔF/F 0 , time to peak (TTP), and rate of rise (ΔF/F 0 .ms − 1 ) of the Ca transient in Sham (open bars, n = 10) and CAL (filled bars, n = 16) myocytes. ⁎⁎⁎ p

    Article Snippet: The blot was probed with anti-LTCC antibody (ACC-003; Alomone, Israel) or anti-GAPDH (G9545; Sigma) and protein bands visualized using relevant peroxidase-conjugated secondary antibodies, chemiluminescence, and autoradiography.

    Techniques: Expressing, Western Blot