cav 1 2 antibody  (Alomone Labs)


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    Alomone Labs cav 1 2 antibody
    Cav 1 2 Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    cav 1 2 antibody  (Alomone Labs)


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  • 96

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    Alomone Labs cav 1 2 antibody
    Cav 1 2 Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    anti ca v 1 2  (Alomone Labs)


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    Alomone Labs anti ca v 1 2
    Anti Ca V 1 2, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ca v 1 2  (Alomone Labs)


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    Alomone Labs ca v 1 2
    List of primers.
    Ca V 1 2, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ca v 1 2/product/Alomone Labs
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    1) Product Images from "Ca v 1.2 Activity and Downstream Signaling Pathways in the Hippocampus of An Animal Model of Depression"

    Article Title: Ca v 1.2 Activity and Downstream Signaling Pathways in the Hippocampus of An Animal Model of Depression

    Journal: Cells

    doi: 10.3390/cells9122609


    Figure Legend Snippet: List of primers.

    Techniques Used:


    Figure Legend Snippet: List of antibodies.

    Techniques Used:

    Chronic stress promotes the expression of Ca v 1.2 subunit in whole hippocampal samples: ( A ) Bar graph of semi-quantitative PCR expression for the Ca v 1.2 mRNA, normalized to 18S mRNA, isolated from whole hippocampus of juvenile rat controls ( n = 8) or subjected to CRS for 3 weeks ( n = 8). ( B ) Bar graph of Ca v 1.2 protein expression from whole hippocampus lysates normalized to α-tubulin ( n = 8), in the upper part, a representative immunoblots showing Ca v 1.2 and α-tubulin protein expression is shown. Bar graphs are mean ± SEM; black bars are control animals, and gray bars correspond to CRS-subjected animals * p value < 0.05.
    Figure Legend Snippet: Chronic stress promotes the expression of Ca v 1.2 subunit in whole hippocampal samples: ( A ) Bar graph of semi-quantitative PCR expression for the Ca v 1.2 mRNA, normalized to 18S mRNA, isolated from whole hippocampus of juvenile rat controls ( n = 8) or subjected to CRS for 3 weeks ( n = 8). ( B ) Bar graph of Ca v 1.2 protein expression from whole hippocampus lysates normalized to α-tubulin ( n = 8), in the upper part, a representative immunoblots showing Ca v 1.2 and α-tubulin protein expression is shown. Bar graphs are mean ± SEM; black bars are control animals, and gray bars correspond to CRS-subjected animals * p value < 0.05.

    Techniques Used: Expressing, Real-time Polymerase Chain Reaction, Isolation, Western Blot

    Calcium channel associated transcriptional regulator (CCAT)-dependent signaling in CRS-subjected animals: ( A ) Schematic diagram depicting the signaling pathway studied in this figure ( B ) Bar graph of 210 kD Ca v 1.2 protein expression normalized to total Ca v 1.2 protein expression (210 kD + 240 kD) from whole hippocampus lysates ( n = 8), in the upper part, a representative immunoblot showing Ca v 1.2 protein expression is shown. Bar graph of semi-quantitative PCR expression for the CCAT mRNA normalized to the Ca v 1.2 mRNA ( C ) or Cx31.1 mRNA ( D ) isolated from whole hippocampus of juvenile rat controls ( n = 8) or subjected to CRS for 3 weeks ( n = 8). Bar graphs are mean ± SEM; black bars are control animals, and gray bars correspond to CRS-subjected animals.
    Figure Legend Snippet: Calcium channel associated transcriptional regulator (CCAT)-dependent signaling in CRS-subjected animals: ( A ) Schematic diagram depicting the signaling pathway studied in this figure ( B ) Bar graph of 210 kD Ca v 1.2 protein expression normalized to total Ca v 1.2 protein expression (210 kD + 240 kD) from whole hippocampus lysates ( n = 8), in the upper part, a representative immunoblot showing Ca v 1.2 protein expression is shown. Bar graph of semi-quantitative PCR expression for the CCAT mRNA normalized to the Ca v 1.2 mRNA ( C ) or Cx31.1 mRNA ( D ) isolated from whole hippocampus of juvenile rat controls ( n = 8) or subjected to CRS for 3 weeks ( n = 8). Bar graphs are mean ± SEM; black bars are control animals, and gray bars correspond to CRS-subjected animals.

    Techniques Used: Expressing, Western Blot, Real-time Polymerase Chain Reaction, Isolation

    cav 1 2 antibody  (Alomone Labs)


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  • 96

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    Alomone Labs cav 1 2 antibody
    Cav 1 2 Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cav 1 2 antibody/product/Alomone Labs
    Average 96 stars, based on 1 article reviews
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    ca v 1 2  (Alomone Labs)


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    Alomone Labs ca v 1 2
    ALPL deficiency caused decreased membrane expression of L-type Ca 2+ channels in BMSCs. a Ca 2+ imaging showed decreased Ca 2+ influx in cultured alpl +/− BMSCs and WT BMSCs transfected with shALP (shALP/WT) after they were stimulated with 30 mmol·L −1 KCl for 3 min ( n = 10). b No KCl-induced Ca 2+ influx was detected in cultured WT, alpl +/− , and shALP/WT BMSCs treated with 10 mmol·L −1 EGTA for 3 min ( n = 10). c ALPL overexpression was mediated by a lentivirus in alpl +/− (Lenti-alp/ alpl +/− ) BMSCs and resulted in an elevated Ca 2+ influx following stimulation with 30 mmol·L −1 KCl for 3 min ( n = 10). d , e The expression of Ca V 1.1, Ca V 1.2, and Ca V 1.3 was assessed. alpl +/− BMSCs showed decreases in total cell expression ( d ) and membrane expression of Ca V 1.2 and Ca V 1.3 ( e ) and no significant change in the levels of cytoplasmic Ca V 1.2 and Ca V 1.3 ( e ). Total Ca V 1.1 protein expression was not changed ( d ), and the expression of membrane and cytoplasmic Ca V 1.1 was not altered in alpl +/− BMSCs ( e ). f Cell-surface biotinylation assay. Left two lanes: western blot for Ca V 1.2 and Ca V 1.3 following neutravidin pull down from WT and alpl +/− BMSCs; right two lanes: input, not biotinylated cells. g Lenti-alp/ alpl +/− BMSCs showed elevated membrane expression of ALP, Ca V 1.2, and Ca V 1.3. h , i Representative images of confocal laser scanning microscopy showing the membrane location of Ca V 1.2 and Ca V 1.3 (green) in WT and Lenti-alp/ alpl +/− BMSCs. The plasma membrane was stained with the marker CellMask™ Deep Red Plasma Membrane Stain (red) ( h ). Quantification of the membrane florescence was performed with NIH ImageJ ( i ). Scale bar, 10 μm. The representative results from three independent experiments are shown. Error bars represent the s.d. from the mean values. * P < 0.05; ** P < 0.01; *** P < 0.001
    Ca V 1 2, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ca v 1 2/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ca v 1 2 - by Bioz Stars, 2023-01
    94/100 stars

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    1) Product Images from "Ionomycin ameliorates hypophosphatasia via rescuing alkaline phosphatase deficiency-mediated L-type Ca 2+ channel internalization in mesenchymal stem cells"

    Article Title: Ionomycin ameliorates hypophosphatasia via rescuing alkaline phosphatase deficiency-mediated L-type Ca 2+ channel internalization in mesenchymal stem cells

    Journal: Bone Research

    doi: 10.1038/s41413-020-0090-7

    ALPL deficiency caused decreased membrane expression of L-type Ca 2+ channels in BMSCs. a Ca 2+ imaging showed decreased Ca 2+ influx in cultured alpl +/− BMSCs and WT BMSCs transfected with shALP (shALP/WT) after they were stimulated with 30 mmol·L −1 KCl for 3 min ( n = 10). b No KCl-induced Ca 2+ influx was detected in cultured WT, alpl +/− , and shALP/WT BMSCs treated with 10 mmol·L −1 EGTA for 3 min ( n = 10). c ALPL overexpression was mediated by a lentivirus in alpl +/− (Lenti-alp/ alpl +/− ) BMSCs and resulted in an elevated Ca 2+ influx following stimulation with 30 mmol·L −1 KCl for 3 min ( n = 10). d , e The expression of Ca V 1.1, Ca V 1.2, and Ca V 1.3 was assessed. alpl +/− BMSCs showed decreases in total cell expression ( d ) and membrane expression of Ca V 1.2 and Ca V 1.3 ( e ) and no significant change in the levels of cytoplasmic Ca V 1.2 and Ca V 1.3 ( e ). Total Ca V 1.1 protein expression was not changed ( d ), and the expression of membrane and cytoplasmic Ca V 1.1 was not altered in alpl +/− BMSCs ( e ). f Cell-surface biotinylation assay. Left two lanes: western blot for Ca V 1.2 and Ca V 1.3 following neutravidin pull down from WT and alpl +/− BMSCs; right two lanes: input, not biotinylated cells. g Lenti-alp/ alpl +/− BMSCs showed elevated membrane expression of ALP, Ca V 1.2, and Ca V 1.3. h , i Representative images of confocal laser scanning microscopy showing the membrane location of Ca V 1.2 and Ca V 1.3 (green) in WT and Lenti-alp/ alpl +/− BMSCs. The plasma membrane was stained with the marker CellMask™ Deep Red Plasma Membrane Stain (red) ( h ). Quantification of the membrane florescence was performed with NIH ImageJ ( i ). Scale bar, 10 μm. The representative results from three independent experiments are shown. Error bars represent the s.d. from the mean values. * P < 0.05; ** P < 0.01; *** P < 0.001
    Figure Legend Snippet: ALPL deficiency caused decreased membrane expression of L-type Ca 2+ channels in BMSCs. a Ca 2+ imaging showed decreased Ca 2+ influx in cultured alpl +/− BMSCs and WT BMSCs transfected with shALP (shALP/WT) after they were stimulated with 30 mmol·L −1 KCl for 3 min ( n = 10). b No KCl-induced Ca 2+ influx was detected in cultured WT, alpl +/− , and shALP/WT BMSCs treated with 10 mmol·L −1 EGTA for 3 min ( n = 10). c ALPL overexpression was mediated by a lentivirus in alpl +/− (Lenti-alp/ alpl +/− ) BMSCs and resulted in an elevated Ca 2+ influx following stimulation with 30 mmol·L −1 KCl for 3 min ( n = 10). d , e The expression of Ca V 1.1, Ca V 1.2, and Ca V 1.3 was assessed. alpl +/− BMSCs showed decreases in total cell expression ( d ) and membrane expression of Ca V 1.2 and Ca V 1.3 ( e ) and no significant change in the levels of cytoplasmic Ca V 1.2 and Ca V 1.3 ( e ). Total Ca V 1.1 protein expression was not changed ( d ), and the expression of membrane and cytoplasmic Ca V 1.1 was not altered in alpl +/− BMSCs ( e ). f Cell-surface biotinylation assay. Left two lanes: western blot for Ca V 1.2 and Ca V 1.3 following neutravidin pull down from WT and alpl +/− BMSCs; right two lanes: input, not biotinylated cells. g Lenti-alp/ alpl +/− BMSCs showed elevated membrane expression of ALP, Ca V 1.2, and Ca V 1.3. h , i Representative images of confocal laser scanning microscopy showing the membrane location of Ca V 1.2 and Ca V 1.3 (green) in WT and Lenti-alp/ alpl +/− BMSCs. The plasma membrane was stained with the marker CellMask™ Deep Red Plasma Membrane Stain (red) ( h ). Quantification of the membrane florescence was performed with NIH ImageJ ( i ). Scale bar, 10 μm. The representative results from three independent experiments are shown. Error bars represent the s.d. from the mean values. * P < 0.05; ** P < 0.01; *** P < 0.001

    Techniques Used: Expressing, Imaging, Cell Culture, Transfection, Over Expression, Cell Surface Biotinylation Assay, Western Blot, Confocal Laser Scanning Microscopy, Staining, Marker

    ALPL-maintained MSC osteogenic/adipogenic lineage differentiation ability via the L-type Ca 2+ channel. a Ca 2+ imaging showed elevated Ca 2+ influx in alpl +/− BMSCs transfected with oeCa V 1.2 or oeCa V 1.3 following stimulation with 30 mmol·L −1 KCl for 3 min ( n = 10). b , c Representative images of confocal laser scanning microscopy showing the membrane location of Ca V 1.2 and Ca V 1.3 (green) in alpl +/− BMSCs transfected with oeCa V 1.2 or oeCa V 1.3. The plasma membrane was stained with the marker CellMask™ Deep Red Plasma Membrane Stain (red) ( b ). Quantification of the membrane florescence was performed with NIH ImageJ ( c ). Scale bar, 10 μm. Alizarin red staining showed that alpl +/− BMSCs transfected with oeCa V 1.2 or oeCa V 1.3 had an increased capacity to form mineralized nodules when cultured under osteoinductive conditions ( d ) and they exhibited an upregulation of the osteogenic-related proteins RUNX2 and Sp7 ( e ). oeCa V 1.2- or oeCa V 1.3-treated alpl +/− BMSCs showed a decreased number of oil red O-positive adipocytes when cultured under adipo-inductive conditions ( f ) and there was a downregulation of the adipogenic-related proteins PPARγ2 and LPL, as assessed by western blot ( g ). h Alizarin red staining showed that alpl +/− BMSCs transfected with siCa V 1.2 or siCa V 1.3 had a decreased capacity to form mineralized nodules when cultured under osteoinductive conditions. i Western blot analysis showed that BMSCs transfected with siCa V 1.2 or siCa V 1.3 expressed decreased levels of the osteogenic-related proteins RUNX2 and Sp7. β-actin was used as a protein loading control. BMSCs transfected with siCa V 1.2 or siCa V 1.3 showed an increased number of oil red O-positive adipocytes when cultured under adipo-inductive conditions ( j ) and upregulation of the adipogenic-related proteins PPARγ2 and LPL, as assessed by western blot ( k ). The representative results from three independent experiments are shown. Error bars represent the s.d. from the mean values. * P < 0.05; ** P < 0.01; *** P < 0.001
    Figure Legend Snippet: ALPL-maintained MSC osteogenic/adipogenic lineage differentiation ability via the L-type Ca 2+ channel. a Ca 2+ imaging showed elevated Ca 2+ influx in alpl +/− BMSCs transfected with oeCa V 1.2 or oeCa V 1.3 following stimulation with 30 mmol·L −1 KCl for 3 min ( n = 10). b , c Representative images of confocal laser scanning microscopy showing the membrane location of Ca V 1.2 and Ca V 1.3 (green) in alpl +/− BMSCs transfected with oeCa V 1.2 or oeCa V 1.3. The plasma membrane was stained with the marker CellMask™ Deep Red Plasma Membrane Stain (red) ( b ). Quantification of the membrane florescence was performed with NIH ImageJ ( c ). Scale bar, 10 μm. Alizarin red staining showed that alpl +/− BMSCs transfected with oeCa V 1.2 or oeCa V 1.3 had an increased capacity to form mineralized nodules when cultured under osteoinductive conditions ( d ) and they exhibited an upregulation of the osteogenic-related proteins RUNX2 and Sp7 ( e ). oeCa V 1.2- or oeCa V 1.3-treated alpl +/− BMSCs showed a decreased number of oil red O-positive adipocytes when cultured under adipo-inductive conditions ( f ) and there was a downregulation of the adipogenic-related proteins PPARγ2 and LPL, as assessed by western blot ( g ). h Alizarin red staining showed that alpl +/− BMSCs transfected with siCa V 1.2 or siCa V 1.3 had a decreased capacity to form mineralized nodules when cultured under osteoinductive conditions. i Western blot analysis showed that BMSCs transfected with siCa V 1.2 or siCa V 1.3 expressed decreased levels of the osteogenic-related proteins RUNX2 and Sp7. β-actin was used as a protein loading control. BMSCs transfected with siCa V 1.2 or siCa V 1.3 showed an increased number of oil red O-positive adipocytes when cultured under adipo-inductive conditions ( j ) and upregulation of the adipogenic-related proteins PPARγ2 and LPL, as assessed by western blot ( k ). The representative results from three independent experiments are shown. Error bars represent the s.d. from the mean values. * P < 0.05; ** P < 0.01; *** P < 0.001

    Techniques Used: Imaging, Transfection, Confocal Laser Scanning Microscopy, Staining, Marker, Cell Culture, Western Blot

    ALPL deficiency promoted the internalization of L-type Ca 2+ channels in BMSCs. a , b Representative images of confocal laser scanning microscopy showing the membrane location of Ca V 1.2 and Ca V 1.3 (green) in alpl +/− BMSCs transfected with DN-Dyn1. The plasma membrane was stained with the marker CellMask™ Deep Red Plasma Membrane Stain (red) ( a ). Quantification of the membrane florescence was performed with NIH ImageJ ( b ). Scale bar, 10 μm. c alpl +/− BMSCs transfected with DN-Dyn1 showed upregulation of Ca V 1.2 and Ca V 1.3 membrane expression and almost no change in the cytoplasmic expression of Ca V 1.2 and Ca V 1.3, as assessed by western blot. β-actin was used as a protein loading control. d 10-min time-lapse confocal laser scanning microscopy images of WT, alpl +/− , DN-Dyn1/ alpl +/− , and Lenti-alp/ alpl +/− BMSCs containing DsRed-CaV1.2. Scale bar, 10 μm. e Ca 2+ imaging showed elevated Ca 2+ influx of cultured alpl +/− BMSCs transfected with DN-Dyn1 after stimulation with 30 mmol·L −1 KCl for 3 min ( n = 10). alpl +/− BMSCs showed a pronounced decrease in DsRed-Ca V 1.2 at the membrane (Dio-labeled ROI, n = 10) ( f ). g Quantification of the florescence in the ROI during the time-course lapse at 0 s, 300 s, and 600 s. h – k Representative images show the colocalization of DsRed-Ca V 1.2 with the Dio-labeled cell membrane of BMSCs. WT, DN-Dyn1/ alpl +/− , and Lenti-alp/ alpl +/− BMSCs had colocalized regions at 0 s and 570 s ( h , j , k ). However, alpl +/− BMSCs showed no colocalization at 0 s and 570 s ( i ). The representative results from three independent experiments are shown. Error bars represent the s.d. from the mean values. * P < 0.05; ** P < 0.01
    Figure Legend Snippet: ALPL deficiency promoted the internalization of L-type Ca 2+ channels in BMSCs. a , b Representative images of confocal laser scanning microscopy showing the membrane location of Ca V 1.2 and Ca V 1.3 (green) in alpl +/− BMSCs transfected with DN-Dyn1. The plasma membrane was stained with the marker CellMask™ Deep Red Plasma Membrane Stain (red) ( a ). Quantification of the membrane florescence was performed with NIH ImageJ ( b ). Scale bar, 10 μm. c alpl +/− BMSCs transfected with DN-Dyn1 showed upregulation of Ca V 1.2 and Ca V 1.3 membrane expression and almost no change in the cytoplasmic expression of Ca V 1.2 and Ca V 1.3, as assessed by western blot. β-actin was used as a protein loading control. d 10-min time-lapse confocal laser scanning microscopy images of WT, alpl +/− , DN-Dyn1/ alpl +/− , and Lenti-alp/ alpl +/− BMSCs containing DsRed-CaV1.2. Scale bar, 10 μm. e Ca 2+ imaging showed elevated Ca 2+ influx of cultured alpl +/− BMSCs transfected with DN-Dyn1 after stimulation with 30 mmol·L −1 KCl for 3 min ( n = 10). alpl +/− BMSCs showed a pronounced decrease in DsRed-Ca V 1.2 at the membrane (Dio-labeled ROI, n = 10) ( f ). g Quantification of the florescence in the ROI during the time-course lapse at 0 s, 300 s, and 600 s. h – k Representative images show the colocalization of DsRed-Ca V 1.2 with the Dio-labeled cell membrane of BMSCs. WT, DN-Dyn1/ alpl +/− , and Lenti-alp/ alpl +/− BMSCs had colocalized regions at 0 s and 570 s ( h , j , k ). However, alpl +/− BMSCs showed no colocalization at 0 s and 570 s ( i ). The representative results from three independent experiments are shown. Error bars represent the s.d. from the mean values. * P < 0.05; ** P < 0.01

    Techniques Used: Confocal Laser Scanning Microscopy, Transfection, Staining, Marker, Expressing, Western Blot, Imaging, Cell Culture, Labeling

    ALPL deficiency promoted the internalization of L-type Ca 2+ channels via binding to α2δ subunits. a Representative images of confocal laser scanning microscopy showing a region of membrane colocalization for ALPL (DsRed) and Ca V 1.2 (FITC-labeled) or Ca V 1.3 (FITC-labeled) in WT BMSCs. No region of membrane colocalization was found in alpl +/− BMSCs. Scale bar, 10 μm. b ALPL immunoprecipitated Ca V 1.2 and Ca V 1.3. The left lane shows the expression of Ca V 1.2 and Ca V 1.3, and the right lane shows the levels of Ca V 1.2 and Ca V 1.3 following immunoprecipitation with an anti-ALPL antibody. c Ca V 1.2 and Ca V 1.3 immunoprecipitated ALPL. The left panel shows the expression of ALPL, and the right panel shows the level of ALPL following immunoprecipitation with anti-Ca V 1.2 or anti-Ca V 1.3 antibodies. d Representative images of confocal laser scanning microscopy showing the membrane colocalization region of ALPL (Cy3-labeled) and Ca V 1.2 (FITC-labeled) or Ca V 1.3 (FITC-labeled) in alpl −/− BMSCs overexpressing ALPL and the α2δ subunit. No membrane colocalization region was found in alpl −/− BMSCs and alpl −/− BMSCs overexpressing ALPL or the mutant α2δ subunit. Scale bar, 10 μm. e Western blot analysis showed membrane expression of Ca V 1.2 or Ca V 1.3 in alpl −/− BMSCs overexpressing ALPL and the α2δ subunit. No membrane expression of Ca V 1.2 or Ca V 1.3 was found in alpl −/− BMSCs and alpl −/− BMSCs overexpressing ALPL or the mutant α2δ subunit. No significant change in cytoplasmic Ca V 1.2 or Ca V 1.3 was found in alpl −/− BMSCs, alpl −/− BMSCs overexpressing ALPL and the mutant α2δ subunit, or alpl −/− BMSCs overexpressing ALPL and the α2δ subunit. f α2δ immunoprecipitated ALPL. The left panel shows the expression of ALPL, and the right panel shows the level of ALPL following immunoprecipitation with an anti-α2δ antibody in alp/ f/f and alpl −/− BMSCs. β-actin was used as a protein loading control. The representative results from three independent experiments are shown
    Figure Legend Snippet: ALPL deficiency promoted the internalization of L-type Ca 2+ channels via binding to α2δ subunits. a Representative images of confocal laser scanning microscopy showing a region of membrane colocalization for ALPL (DsRed) and Ca V 1.2 (FITC-labeled) or Ca V 1.3 (FITC-labeled) in WT BMSCs. No region of membrane colocalization was found in alpl +/− BMSCs. Scale bar, 10 μm. b ALPL immunoprecipitated Ca V 1.2 and Ca V 1.3. The left lane shows the expression of Ca V 1.2 and Ca V 1.3, and the right lane shows the levels of Ca V 1.2 and Ca V 1.3 following immunoprecipitation with an anti-ALPL antibody. c Ca V 1.2 and Ca V 1.3 immunoprecipitated ALPL. The left panel shows the expression of ALPL, and the right panel shows the level of ALPL following immunoprecipitation with anti-Ca V 1.2 or anti-Ca V 1.3 antibodies. d Representative images of confocal laser scanning microscopy showing the membrane colocalization region of ALPL (Cy3-labeled) and Ca V 1.2 (FITC-labeled) or Ca V 1.3 (FITC-labeled) in alpl −/− BMSCs overexpressing ALPL and the α2δ subunit. No membrane colocalization region was found in alpl −/− BMSCs and alpl −/− BMSCs overexpressing ALPL or the mutant α2δ subunit. Scale bar, 10 μm. e Western blot analysis showed membrane expression of Ca V 1.2 or Ca V 1.3 in alpl −/− BMSCs overexpressing ALPL and the α2δ subunit. No membrane expression of Ca V 1.2 or Ca V 1.3 was found in alpl −/− BMSCs and alpl −/− BMSCs overexpressing ALPL or the mutant α2δ subunit. No significant change in cytoplasmic Ca V 1.2 or Ca V 1.3 was found in alpl −/− BMSCs, alpl −/− BMSCs overexpressing ALPL and the mutant α2δ subunit, or alpl −/− BMSCs overexpressing ALPL and the α2δ subunit. f α2δ immunoprecipitated ALPL. The left panel shows the expression of ALPL, and the right panel shows the level of ALPL following immunoprecipitation with an anti-α2δ antibody in alp/ f/f and alpl −/− BMSCs. β-actin was used as a protein loading control. The representative results from three independent experiments are shown

    Techniques Used: Binding Assay, Confocal Laser Scanning Microscopy, Labeling, Immunoprecipitation, Expressing, Mutagenesis, Western Blot

    ALPL deficiency promoted the internalization of L-type Ca 2+ channels in HPP patient-derived BMSCs. a The expression of ALPL on the membrane and cytoplasm was decreased in BMSCs from two HPP patients compared with that of normal human BMSCs. β-actin was used as a protein loading control. b Intracellular Ca 2+ imaging analysis showed that KCl-induced Ca 2+ influx was significantly decreased in cultured BMSCs from HPP patients compared with that of normal human BMSCs. c Overexpression of ALPL or transfection with DN-Dyn1 in BMSCs from A1 patients showed elevated KCl-induced Ca 2+ influx. d Overexpression of ALPL or transfection with DN-Dyn1 in BMSCs from A2 patients showed elevated KCl-induced Ca 2+ influx. e Representative images of confocal laser scanning microscopy showing the membrane location of Ca V 1.2 and Ca V 1.3 in control BMSCs, A1 BMSCs, A2 BMSCs, A1 and A2 BMSCs overexpressing ALPL, and A1 and A2 BMSCs transfected with DN-Dyn1. Scale bar, 10 μm. The representative results from three independent experiments are shown. Error bars represent the s.d. from the mean values. * P < 0.05; ** P < 0.01
    Figure Legend Snippet: ALPL deficiency promoted the internalization of L-type Ca 2+ channels in HPP patient-derived BMSCs. a The expression of ALPL on the membrane and cytoplasm was decreased in BMSCs from two HPP patients compared with that of normal human BMSCs. β-actin was used as a protein loading control. b Intracellular Ca 2+ imaging analysis showed that KCl-induced Ca 2+ influx was significantly decreased in cultured BMSCs from HPP patients compared with that of normal human BMSCs. c Overexpression of ALPL or transfection with DN-Dyn1 in BMSCs from A1 patients showed elevated KCl-induced Ca 2+ influx. d Overexpression of ALPL or transfection with DN-Dyn1 in BMSCs from A2 patients showed elevated KCl-induced Ca 2+ influx. e Representative images of confocal laser scanning microscopy showing the membrane location of Ca V 1.2 and Ca V 1.3 in control BMSCs, A1 BMSCs, A2 BMSCs, A1 and A2 BMSCs overexpressing ALPL, and A1 and A2 BMSCs transfected with DN-Dyn1. Scale bar, 10 μm. The representative results from three independent experiments are shown. Error bars represent the s.d. from the mean values. * P < 0.05; ** P < 0.01

    Techniques Used: Derivative Assay, Expressing, Imaging, Cell Culture, Over Expression, Transfection, Confocal Laser Scanning Microscopy

    ca v 2 1  (Alomone Labs)


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    Structured Review

    Alomone Labs ca v 2 1
    Ca V 2 1, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ca v 1 2  (Alomone Labs)


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    Alomone Labs ca v 1 2
    Ca V 1.2 protein levels in rat brain and body weight. Ca V 1.2 protein expression levels in the right prefrontal cortex and hippocampus of 2-month-old male Cacna1c +/– rats and Cacna1c +/+ littermate controls from the different environmental conditions were analyzed by Western blot. (A) One representative immunoblot per brain area is shown. (B) The bar graphs were obtained by densitometric quantification of the Western blot data. The values are normalized to the loading control vinculin and presented as fold of Cacna1c +/+ -Stand (mean ± SD, n = 6). (C) Whole body weight was determined after the 4-week exposure to the experimental housing conditions at ∼2 months of age (mean ± SD, n = 8–9). Statistical significance is highlighted by an asterisk ( ∗ ). +/+, wildtype Cacna1c +/+ ; + ⁣/⁣−, heterozygous Cacna1c +/– ; Iso, isolation; Stand, standard housing; Enr, enrichment.
    Ca V 1 2, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ca v 1 2/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ca v 1 2 - by Bioz Stars, 2023-01
    94/100 stars

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    1) Product Images from "Interaction of the Psychiatric Risk Gene Cacna1c With Post-weaning Social Isolation or Environmental Enrichment Does Not Affect Brain Mitochondrial Bioenergetics in Rats"

    Article Title: Interaction of the Psychiatric Risk Gene Cacna1c With Post-weaning Social Isolation or Environmental Enrichment Does Not Affect Brain Mitochondrial Bioenergetics in Rats

    Journal: Frontiers in Cellular Neuroscience

    doi: 10.3389/fncel.2019.00483

    Ca V 1.2 protein levels in rat brain and body weight. Ca V 1.2 protein expression levels in the right prefrontal cortex and hippocampus of 2-month-old male Cacna1c +/– rats and Cacna1c +/+ littermate controls from the different environmental conditions were analyzed by Western blot. (A) One representative immunoblot per brain area is shown. (B) The bar graphs were obtained by densitometric quantification of the Western blot data. The values are normalized to the loading control vinculin and presented as fold of Cacna1c +/+ -Stand (mean ± SD, n = 6). (C) Whole body weight was determined after the 4-week exposure to the experimental housing conditions at ∼2 months of age (mean ± SD, n = 8–9). Statistical significance is highlighted by an asterisk ( ∗ ). +/+, wildtype Cacna1c +/+ ; + ⁣/⁣−, heterozygous Cacna1c +/– ; Iso, isolation; Stand, standard housing; Enr, enrichment.
    Figure Legend Snippet: Ca V 1.2 protein levels in rat brain and body weight. Ca V 1.2 protein expression levels in the right prefrontal cortex and hippocampus of 2-month-old male Cacna1c +/– rats and Cacna1c +/+ littermate controls from the different environmental conditions were analyzed by Western blot. (A) One representative immunoblot per brain area is shown. (B) The bar graphs were obtained by densitometric quantification of the Western blot data. The values are normalized to the loading control vinculin and presented as fold of Cacna1c +/+ -Stand (mean ± SD, n = 6). (C) Whole body weight was determined after the 4-week exposure to the experimental housing conditions at ∼2 months of age (mean ± SD, n = 8–9). Statistical significance is highlighted by an asterisk ( ∗ ). +/+, wildtype Cacna1c +/+ ; + ⁣/⁣−, heterozygous Cacna1c +/– ; Iso, isolation; Stand, standard housing; Enr, enrichment.

    Techniques Used: Expressing, Western Blot, Isolation

    rabbit anti cav 1 2  (Alomone Labs)


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    Alomone Labs rabbit anti cav 1 2
    Rabbit Anti Cav 1 2, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    rabbit anti cav 1 2  (Alomone Labs)


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    Alomone Labs rabbit anti cav 1 2
    Rabbit Anti Cav 1 2, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    cav 1 2  (Alomone Labs)


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    Alomone Labs cav 1 2
    Cav 1 2, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Alomone Labs cav 1 2 antibody
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    Image Search Results


    Journal: Cells

    Article Title: Ca v 1.2 Activity and Downstream Signaling Pathways in the Hippocampus of An Animal Model of Depression

    doi: 10.3390/cells9122609

    Figure Lengend Snippet: List of primers.

    Article Snippet: Ca v 1.2 , pAB , 1:200 , Alomone , ACC-003.

    Techniques:

    Chronic stress promotes the expression of Ca v 1.2 subunit in whole hippocampal samples: ( A ) Bar graph of semi-quantitative PCR expression for the Ca v 1.2 mRNA, normalized to 18S mRNA, isolated from whole hippocampus of juvenile rat controls ( n = 8) or subjected to CRS for 3 weeks ( n = 8). ( B ) Bar graph of Ca v 1.2 protein expression from whole hippocampus lysates normalized to α-tubulin ( n = 8), in the upper part, a representative immunoblots showing Ca v 1.2 and α-tubulin protein expression is shown. Bar graphs are mean ± SEM; black bars are control animals, and gray bars correspond to CRS-subjected animals * p value < 0.05.

    Journal: Cells

    Article Title: Ca v 1.2 Activity and Downstream Signaling Pathways in the Hippocampus of An Animal Model of Depression

    doi: 10.3390/cells9122609

    Figure Lengend Snippet: Chronic stress promotes the expression of Ca v 1.2 subunit in whole hippocampal samples: ( A ) Bar graph of semi-quantitative PCR expression for the Ca v 1.2 mRNA, normalized to 18S mRNA, isolated from whole hippocampus of juvenile rat controls ( n = 8) or subjected to CRS for 3 weeks ( n = 8). ( B ) Bar graph of Ca v 1.2 protein expression from whole hippocampus lysates normalized to α-tubulin ( n = 8), in the upper part, a representative immunoblots showing Ca v 1.2 and α-tubulin protein expression is shown. Bar graphs are mean ± SEM; black bars are control animals, and gray bars correspond to CRS-subjected animals * p value < 0.05.

    Article Snippet: Ca v 1.2 , pAB , 1:200 , Alomone , ACC-003.

    Techniques: Expressing, Real-time Polymerase Chain Reaction, Isolation, Western Blot

    Calcium channel associated transcriptional regulator (CCAT)-dependent signaling in CRS-subjected animals: ( A ) Schematic diagram depicting the signaling pathway studied in this figure ( B ) Bar graph of 210 kD Ca v 1.2 protein expression normalized to total Ca v 1.2 protein expression (210 kD + 240 kD) from whole hippocampus lysates ( n = 8), in the upper part, a representative immunoblot showing Ca v 1.2 protein expression is shown. Bar graph of semi-quantitative PCR expression for the CCAT mRNA normalized to the Ca v 1.2 mRNA ( C ) or Cx31.1 mRNA ( D ) isolated from whole hippocampus of juvenile rat controls ( n = 8) or subjected to CRS for 3 weeks ( n = 8). Bar graphs are mean ± SEM; black bars are control animals, and gray bars correspond to CRS-subjected animals.

    Journal: Cells

    Article Title: Ca v 1.2 Activity and Downstream Signaling Pathways in the Hippocampus of An Animal Model of Depression

    doi: 10.3390/cells9122609

    Figure Lengend Snippet: Calcium channel associated transcriptional regulator (CCAT)-dependent signaling in CRS-subjected animals: ( A ) Schematic diagram depicting the signaling pathway studied in this figure ( B ) Bar graph of 210 kD Ca v 1.2 protein expression normalized to total Ca v 1.2 protein expression (210 kD + 240 kD) from whole hippocampus lysates ( n = 8), in the upper part, a representative immunoblot showing Ca v 1.2 protein expression is shown. Bar graph of semi-quantitative PCR expression for the CCAT mRNA normalized to the Ca v 1.2 mRNA ( C ) or Cx31.1 mRNA ( D ) isolated from whole hippocampus of juvenile rat controls ( n = 8) or subjected to CRS for 3 weeks ( n = 8). Bar graphs are mean ± SEM; black bars are control animals, and gray bars correspond to CRS-subjected animals.

    Article Snippet: Ca v 1.2 , pAB , 1:200 , Alomone , ACC-003.

    Techniques: Expressing, Western Blot, Real-time Polymerase Chain Reaction, Isolation