anti cav 2 1  (Alomone Labs)


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    Structured Review

    Alomone Labs anti cav 2 1
    AβOs bind to axons and colocalize with presynaptic voltage-gated calcium channels. (A) Representative images of immunocytochemistry with an AβO-specific antibody (NU-4) show that AβOs bind along the entire length of the axon in cultured neurons. (B) Immunohistochemistry on coronal sections from 12-mo-old transgenic AD mouse brain (LaFerla 3xTg) reveal a punctate distribution of AβOs along axons in the cortex. Axons are distinguished by the absence of MAP2 staining (inset). AβOs were not detected in age-matched wild-type control mice. (C) Representative images of AβO and CaV 2.1 (P/Q-type VGCC) immunocytochemistry. Of axonal AβOs, 83.5% colocalize with P/Q-type VGCCs. A minimum of 15 cells from three independent cultures were analyzed. Arrows indicate axons, arrowheads indicate dendrites, and asterisks indicate regions of overlapping puncta. Scale bar, 100 μm.
    Anti Cav 2 1, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "Dendritic and axonal mechanisms of Ca2+ elevation impair BDNF transport in Aβ oligomer–treated hippocampal neurons"

    Article Title: Dendritic and axonal mechanisms of Ca2+ elevation impair BDNF transport in Aβ oligomer–treated hippocampal neurons

    Journal: Molecular Biology of the Cell

    doi: 10.1091/mbc.E14-12-1612

    AβOs bind to axons and colocalize with presynaptic voltage-gated calcium channels. (A) Representative images of immunocytochemistry with an AβO-specific antibody (NU-4) show that AβOs bind along the entire length of the axon in cultured neurons. (B) Immunohistochemistry on coronal sections from 12-mo-old transgenic AD mouse brain (LaFerla 3xTg) reveal a punctate distribution of AβOs along axons in the cortex. Axons are distinguished by the absence of MAP2 staining (inset). AβOs were not detected in age-matched wild-type control mice. (C) Representative images of AβO and CaV 2.1 (P/Q-type VGCC) immunocytochemistry. Of axonal AβOs, 83.5% colocalize with P/Q-type VGCCs. A minimum of 15 cells from three independent cultures were analyzed. Arrows indicate axons, arrowheads indicate dendrites, and asterisks indicate regions of overlapping puncta. Scale bar, 100 μm.
    Figure Legend Snippet: AβOs bind to axons and colocalize with presynaptic voltage-gated calcium channels. (A) Representative images of immunocytochemistry with an AβO-specific antibody (NU-4) show that AβOs bind along the entire length of the axon in cultured neurons. (B) Immunohistochemistry on coronal sections from 12-mo-old transgenic AD mouse brain (LaFerla 3xTg) reveal a punctate distribution of AβOs along axons in the cortex. Axons are distinguished by the absence of MAP2 staining (inset). AβOs were not detected in age-matched wild-type control mice. (C) Representative images of AβO and CaV 2.1 (P/Q-type VGCC) immunocytochemistry. Of axonal AβOs, 83.5% colocalize with P/Q-type VGCCs. A minimum of 15 cells from three independent cultures were analyzed. Arrows indicate axons, arrowheads indicate dendrites, and asterisks indicate regions of overlapping puncta. Scale bar, 100 μm.

    Techniques Used: Immunocytochemistry, Cell Culture, Immunohistochemistry, Transgenic Assay, Staining, Mouse Assay

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    Alomone Labs 2 2 n type calcium channels
    Interactions of MAP6‐E mutants with Ca v <t>2.2/N‐type</t> calcium channels and Tctex1, and restoration of neuritic content. (A) HEK‐293 T17 cells were cotransfected with plasmids encoding NCD4‐myc and full‐length MAP6‐E or mutants as in Fig. 1 F. Each immunoprecipitation was performed with the anti‐MAP6 23N antibody and immunorevealed by Western blotting against myc or MAP6 (23N). Note that the many MAP6 bands detected after immunoprecipitation correspond to specific degradation products as there is no signal in the sham‐transfected condition. NCD4‐related bands were quantified, background subtracted and normalized by that obtained with non‐mutated MAP6‐E (italic percentages). (B) HEK‐293 T17 cells were cotransfected with plasmids encoding NCD4‐myc, Tctex1‐GFP and full‐length MAP6‐E or mutants as in Fig. 1 F. Each immunoprecipitation was performed with the anti‐MAP6 23N antibody and immunorevealed by Western blotting against myc, GFP or MAP6 (23N). Note that the many MAP6 bands detected after immunoprecipitation correspond to specific degradation products as there is no signal in the sham‐transfected condition. NCD4‐related bands and Tctex1‐related bands were quantified, background subtracted and normalized by that obtained with non‐mutated MAP6‐E (italic percentages). (C) Quantification of neuritic Ca v 2.2/N‐type calcium channels contents from WT (white discs) and MAP6 KO (grey discs) hippocampal neurons cotransfected with plasmids encoding GFP as in Fig. 4 C and full‐length MAP6‐E or mutants as indicated, normalized by sham‐transfected neurons of corresponding genotype (dashed grey line = 100%). n represents the total number of transfected neurons measured from three independent neuronal cultures. ns, not significant and **, P
    2 2 N Type Calcium Channels, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Alomone Labs anti cav 2 1
    AβOs bind to axons and colocalize with presynaptic voltage-gated calcium channels. (A) Representative images of immunocytochemistry with an AβO-specific antibody (NU-4) show that AβOs bind along the entire length of the axon in cultured neurons. (B) Immunohistochemistry on coronal sections from 12-mo-old transgenic AD mouse brain (LaFerla 3xTg) reveal a punctate distribution of AβOs along axons in the cortex. Axons are distinguished by the absence of MAP2 staining (inset). AβOs were not detected in age-matched wild-type control mice. (C) Representative images of AβO and CaV 2.1 (P/Q-type VGCC) immunocytochemistry. Of axonal AβOs, 83.5% colocalize with P/Q-type VGCCs. A minimum of 15 cells from three independent cultures were analyzed. Arrows indicate axons, arrowheads indicate dendrites, and asterisks indicate regions of overlapping puncta. Scale bar, 100 μm.
    Anti Cav 2 1, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti cav 2 1/product/Alomone Labs
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
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    96
    Alomone Labs rabbit anti cav 1 2
    AβOs bind to axons and colocalize with presynaptic voltage-gated calcium channels. (A) Representative images of immunocytochemistry with an AβO-specific antibody (NU-4) show that AβOs bind along the entire length of the axon in cultured neurons. (B) Immunohistochemistry on coronal sections from 12-mo-old transgenic AD mouse brain (LaFerla 3xTg) reveal a punctate distribution of AβOs along axons in the cortex. Axons are distinguished by the absence of MAP2 staining (inset). AβOs were not detected in age-matched wild-type control mice. (C) Representative images of AβO and CaV 2.1 (P/Q-type VGCC) immunocytochemistry. Of axonal AβOs, 83.5% colocalize with P/Q-type VGCCs. A minimum of 15 cells from three independent cultures were analyzed. Arrows indicate axons, arrowheads indicate dendrites, and asterisks indicate regions of overlapping puncta. Scale bar, 100 μm.
    Rabbit Anti Cav 1 2, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti cav 1 2/product/Alomone Labs
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    Image Search Results


    Interactions of MAP6‐E mutants with Ca v 2.2/N‐type calcium channels and Tctex1, and restoration of neuritic content. (A) HEK‐293 T17 cells were cotransfected with plasmids encoding NCD4‐myc and full‐length MAP6‐E or mutants as in Fig. 1 F. Each immunoprecipitation was performed with the anti‐MAP6 23N antibody and immunorevealed by Western blotting against myc or MAP6 (23N). Note that the many MAP6 bands detected after immunoprecipitation correspond to specific degradation products as there is no signal in the sham‐transfected condition. NCD4‐related bands were quantified, background subtracted and normalized by that obtained with non‐mutated MAP6‐E (italic percentages). (B) HEK‐293 T17 cells were cotransfected with plasmids encoding NCD4‐myc, Tctex1‐GFP and full‐length MAP6‐E or mutants as in Fig. 1 F. Each immunoprecipitation was performed with the anti‐MAP6 23N antibody and immunorevealed by Western blotting against myc, GFP or MAP6 (23N). Note that the many MAP6 bands detected after immunoprecipitation correspond to specific degradation products as there is no signal in the sham‐transfected condition. NCD4‐related bands and Tctex1‐related bands were quantified, background subtracted and normalized by that obtained with non‐mutated MAP6‐E (italic percentages). (C) Quantification of neuritic Ca v 2.2/N‐type calcium channels contents from WT (white discs) and MAP6 KO (grey discs) hippocampal neurons cotransfected with plasmids encoding GFP as in Fig. 4 C and full‐length MAP6‐E or mutants as indicated, normalized by sham‐transfected neurons of corresponding genotype (dashed grey line = 100%). n represents the total number of transfected neurons measured from three independent neuronal cultures. ns, not significant and **, P

    Journal: The European Journal of Neuroscience

    Article Title: MAP6 interacts with Tctex1 and Cav2.2/N‐type calcium channels to regulate calcium signalling in neurons

    doi: 10.1111/ejn.13766

    Figure Lengend Snippet: Interactions of MAP6‐E mutants with Ca v 2.2/N‐type calcium channels and Tctex1, and restoration of neuritic content. (A) HEK‐293 T17 cells were cotransfected with plasmids encoding NCD4‐myc and full‐length MAP6‐E or mutants as in Fig. 1 F. Each immunoprecipitation was performed with the anti‐MAP6 23N antibody and immunorevealed by Western blotting against myc or MAP6 (23N). Note that the many MAP6 bands detected after immunoprecipitation correspond to specific degradation products as there is no signal in the sham‐transfected condition. NCD4‐related bands were quantified, background subtracted and normalized by that obtained with non‐mutated MAP6‐E (italic percentages). (B) HEK‐293 T17 cells were cotransfected with plasmids encoding NCD4‐myc, Tctex1‐GFP and full‐length MAP6‐E or mutants as in Fig. 1 F. Each immunoprecipitation was performed with the anti‐MAP6 23N antibody and immunorevealed by Western blotting against myc, GFP or MAP6 (23N). Note that the many MAP6 bands detected after immunoprecipitation correspond to specific degradation products as there is no signal in the sham‐transfected condition. NCD4‐related bands and Tctex1‐related bands were quantified, background subtracted and normalized by that obtained with non‐mutated MAP6‐E (italic percentages). (C) Quantification of neuritic Ca v 2.2/N‐type calcium channels contents from WT (white discs) and MAP6 KO (grey discs) hippocampal neurons cotransfected with plasmids encoding GFP as in Fig. 4 C and full‐length MAP6‐E or mutants as indicated, normalized by sham‐transfected neurons of corresponding genotype (dashed grey line = 100%). n represents the total number of transfected neurons measured from three independent neuronal cultures. ns, not significant and **, P

    Article Snippet: Brain extracts migrated on SDS‐PAGE gels were transferred onto nitrocellulose membranes; membranes were then cut to enable immunodetection of Cav 2.2/N‐type calcium channels (ACC‐002, rabbit, Alomone Labs, Israel, 1/500) and neuron‐specific enolase (AB9698, chicken, Merck Millipore, France, 1/2500), a MAP6‐insensitive marker, from the same extract.

    Techniques: Immunoprecipitation, Western Blot, Transfection

    Calcium channel expression and traffic in MAP6 KO neurons. (A) Left panels, example of Western blotting of Ca v 2.2/N‐type calcium channels and Neuron‐specific enolase (NSE) obtained from WT and MAP6 KO hippocampal extracts. Right panel, quantification of C a v 2.2/N‐type channels normalized to NSE ratios obtained from cerebella, cortices and hippocampi of WT (white discs) and MAP6 KO (grey discs) animals. ns, P > 0.05 against the corresponding WT, using unpaired parametric t ‐tests. (B) Left panels, immunolabelling of microtubules (green) and Ca v 2.2/N‐type calcium channels (magenta) and detection of spots on a merged image using ImageJ (see Supporting Information for details). Scale bar = 10 μm. Right panel, quantification of Ca v 2.2/N‐type calcium channels from WT (white discs) and MAP6 KO (grey discs) hippocampal neurons, normalized by that of WT neurons (dashed grey line = 100%) for each neuronal culture. n represents the total number of individual embryos used during four independent preparations of neuronal cultures. ns, P > 0.05 as compared to WT, using an unpaired parametric t ‐test. (C) Left panel, example of a neurite from a GFP‐transfected WT neuron (green) with Ca v 2.2/N‐type calcium channels immunodetection (magenta) and measurement of neuritic content on a merged image using ImageJ (white, see Supporting Information for details). Scale bar = 10 μm. Right panel, quantification of neuritic Ca v 2.2/N‐type calcium channels content from GFP‐transfected WT (white discs) and MAP6 KO (grey discs) hippocampal neurons, normalized by that of WT neurons (dashed grey line = 100%). n represents the total number of transfected neurons measured from three independent neuronal cultures. ***, P

    Journal: The European Journal of Neuroscience

    Article Title: MAP6 interacts with Tctex1 and Cav2.2/N‐type calcium channels to regulate calcium signalling in neurons

    doi: 10.1111/ejn.13766

    Figure Lengend Snippet: Calcium channel expression and traffic in MAP6 KO neurons. (A) Left panels, example of Western blotting of Ca v 2.2/N‐type calcium channels and Neuron‐specific enolase (NSE) obtained from WT and MAP6 KO hippocampal extracts. Right panel, quantification of C a v 2.2/N‐type channels normalized to NSE ratios obtained from cerebella, cortices and hippocampi of WT (white discs) and MAP6 KO (grey discs) animals. ns, P > 0.05 against the corresponding WT, using unpaired parametric t ‐tests. (B) Left panels, immunolabelling of microtubules (green) and Ca v 2.2/N‐type calcium channels (magenta) and detection of spots on a merged image using ImageJ (see Supporting Information for details). Scale bar = 10 μm. Right panel, quantification of Ca v 2.2/N‐type calcium channels from WT (white discs) and MAP6 KO (grey discs) hippocampal neurons, normalized by that of WT neurons (dashed grey line = 100%) for each neuronal culture. n represents the total number of individual embryos used during four independent preparations of neuronal cultures. ns, P > 0.05 as compared to WT, using an unpaired parametric t ‐test. (C) Left panel, example of a neurite from a GFP‐transfected WT neuron (green) with Ca v 2.2/N‐type calcium channels immunodetection (magenta) and measurement of neuritic content on a merged image using ImageJ (white, see Supporting Information for details). Scale bar = 10 μm. Right panel, quantification of neuritic Ca v 2.2/N‐type calcium channels content from GFP‐transfected WT (white discs) and MAP6 KO (grey discs) hippocampal neurons, normalized by that of WT neurons (dashed grey line = 100%). n represents the total number of transfected neurons measured from three independent neuronal cultures. ***, P

    Article Snippet: Brain extracts migrated on SDS‐PAGE gels were transferred onto nitrocellulose membranes; membranes were then cut to enable immunodetection of Cav 2.2/N‐type calcium channels (ACC‐002, rabbit, Alomone Labs, Israel, 1/500) and neuron‐specific enolase (AB9698, chicken, Merck Millipore, France, 1/2500), a MAP6‐insensitive marker, from the same extract.

    Techniques: Expressing, Western Blot, Transfection, Immunodetection

    AβOs bind to axons and colocalize with presynaptic voltage-gated calcium channels. (A) Representative images of immunocytochemistry with an AβO-specific antibody (NU-4) show that AβOs bind along the entire length of the axon in cultured neurons. (B) Immunohistochemistry on coronal sections from 12-mo-old transgenic AD mouse brain (LaFerla 3xTg) reveal a punctate distribution of AβOs along axons in the cortex. Axons are distinguished by the absence of MAP2 staining (inset). AβOs were not detected in age-matched wild-type control mice. (C) Representative images of AβO and CaV 2.1 (P/Q-type VGCC) immunocytochemistry. Of axonal AβOs, 83.5% colocalize with P/Q-type VGCCs. A minimum of 15 cells from three independent cultures were analyzed. Arrows indicate axons, arrowheads indicate dendrites, and asterisks indicate regions of overlapping puncta. Scale bar, 100 μm.

    Journal: Molecular Biology of the Cell

    Article Title: Dendritic and axonal mechanisms of Ca2+ elevation impair BDNF transport in Aβ oligomer–treated hippocampal neurons

    doi: 10.1091/mbc.E14-12-1612

    Figure Lengend Snippet: AβOs bind to axons and colocalize with presynaptic voltage-gated calcium channels. (A) Representative images of immunocytochemistry with an AβO-specific antibody (NU-4) show that AβOs bind along the entire length of the axon in cultured neurons. (B) Immunohistochemistry on coronal sections from 12-mo-old transgenic AD mouse brain (LaFerla 3xTg) reveal a punctate distribution of AβOs along axons in the cortex. Axons are distinguished by the absence of MAP2 staining (inset). AβOs were not detected in age-matched wild-type control mice. (C) Representative images of AβO and CaV 2.1 (P/Q-type VGCC) immunocytochemistry. Of axonal AβOs, 83.5% colocalize with P/Q-type VGCCs. A minimum of 15 cells from three independent cultures were analyzed. Arrows indicate axons, arrowheads indicate dendrites, and asterisks indicate regions of overlapping puncta. Scale bar, 100 μm.

    Article Snippet: To determine AβO colocalization with VGCCs, AβO-treated cells were stained with anti-CaV 2.1, anti-CaV 2.2, and anti-CaV 2.3 (1:100; Alomone Labs).

    Techniques: Immunocytochemistry, Cell Culture, Immunohistochemistry, Transgenic Assay, Staining, Mouse Assay