anti caspase 3  (Abcam)

 
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    Name:
    Anti Caspase 3 antibody
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    Catalog Number:
    AB13847
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    Structured Review

    Abcam anti caspase 3
    Sulforaphane synergizes with cisplatin in suppressing cancer cell proliferation and promoting apoptosis of ovarian cancer cells. (A) Synergism between sulforaphane and cisplatin. A2780 cells were treated with sulforaphane and cisplatin at the indicated concentrations. After 24 h, WST-1 reagent was administrated to the culture medium and then incubated for 1 h. The WST-1 activities were determined at 440 nm. (B) Colony formation assay. Subconfluent A2780 cells were seeded in plates and treated with sulforaphane and cisplatin at the indicated concentrations. After 48 h, the cancer cells were then fixed and stained with crystal violet for the colony formation assay. (C) Colony-forming ability of ovarian cancer cells following sulforaphane and cisplatin treatment was quantified for optical absorbance. (D) Western blot assays with (E) quantification were performed to determine protein levels of Bcl-2, <t>Caspase-3,</t> P53, and Cyclin-D1 in A2780 cells treated with or without sulforaphane and cisplatin. The values are presented as the mean ± standard deviation (n=8-10) of the samples. + P

    https://www.bioz.com/result/anti caspase 3/product/Abcam
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti caspase 3 - by Bioz Stars, 2021-06
    99/100 stars

    Images

    1) Product Images from "Sulforaphane regulates apoptosis- and proliferation-related signaling pathways and synergizes with cisplatin to suppress human ovarian cancer"

    Article Title: Sulforaphane regulates apoptosis- and proliferation-related signaling pathways and synergizes with cisplatin to suppress human ovarian cancer

    Journal: International Journal of Molecular Medicine

    doi: 10.3892/ijmm.2018.3860

    Sulforaphane synergizes with cisplatin in suppressing cancer cell proliferation and promoting apoptosis of ovarian cancer cells. (A) Synergism between sulforaphane and cisplatin. A2780 cells were treated with sulforaphane and cisplatin at the indicated concentrations. After 24 h, WST-1 reagent was administrated to the culture medium and then incubated for 1 h. The WST-1 activities were determined at 440 nm. (B) Colony formation assay. Subconfluent A2780 cells were seeded in plates and treated with sulforaphane and cisplatin at the indicated concentrations. After 48 h, the cancer cells were then fixed and stained with crystal violet for the colony formation assay. (C) Colony-forming ability of ovarian cancer cells following sulforaphane and cisplatin treatment was quantified for optical absorbance. (D) Western blot assays with (E) quantification were performed to determine protein levels of Bcl-2, Caspase-3, P53, and Cyclin-D1 in A2780 cells treated with or without sulforaphane and cisplatin. The values are presented as the mean ± standard deviation (n=8-10) of the samples. + P
    Figure Legend Snippet: Sulforaphane synergizes with cisplatin in suppressing cancer cell proliferation and promoting apoptosis of ovarian cancer cells. (A) Synergism between sulforaphane and cisplatin. A2780 cells were treated with sulforaphane and cisplatin at the indicated concentrations. After 24 h, WST-1 reagent was administrated to the culture medium and then incubated for 1 h. The WST-1 activities were determined at 440 nm. (B) Colony formation assay. Subconfluent A2780 cells were seeded in plates and treated with sulforaphane and cisplatin at the indicated concentrations. After 48 h, the cancer cells were then fixed and stained with crystal violet for the colony formation assay. (C) Colony-forming ability of ovarian cancer cells following sulforaphane and cisplatin treatment was quantified for optical absorbance. (D) Western blot assays with (E) quantification were performed to determine protein levels of Bcl-2, Caspase-3, P53, and Cyclin-D1 in A2780 cells treated with or without sulforaphane and cisplatin. The values are presented as the mean ± standard deviation (n=8-10) of the samples. + P

    Techniques Used: Incubation, Colony Assay, Staining, Western Blot, Standard Deviation

    Sulforaphane inhibits multiple cancer development-associated signaling pathways in A2780 cells. (A) Effect of sulforaphane on intracellular levels of Her2 through immunofluorescence assays (magnification, ×100). (B) Western blot assays were performed to examine the effect of sulforaphane on the 10 cancer-associated signaling pathway reporter activities, including Bcl-2, Bax, Cyto-c, Caspase-3, p-AKT, p-NF-κB, P53, P27, cyclin-D1 and cMyc. The values are presented as the mean ± standard deviation (n=8-10) of the samples. + P
    Figure Legend Snippet: Sulforaphane inhibits multiple cancer development-associated signaling pathways in A2780 cells. (A) Effect of sulforaphane on intracellular levels of Her2 through immunofluorescence assays (magnification, ×100). (B) Western blot assays were performed to examine the effect of sulforaphane on the 10 cancer-associated signaling pathway reporter activities, including Bcl-2, Bax, Cyto-c, Caspase-3, p-AKT, p-NF-κB, P53, P27, cyclin-D1 and cMyc. The values are presented as the mean ± standard deviation (n=8-10) of the samples. + P

    Techniques Used: Immunofluorescence, Western Blot, Standard Deviation

    2) Product Images from "Protective Effects of Total Saponins of Aralia elata (Miq.) on Endothelial Cell Injury Induced by TNF-α via Modulation of the PI3K/Akt and NF-κB Signalling Pathways"

    Article Title: Protective Effects of Total Saponins of Aralia elata (Miq.) on Endothelial Cell Injury Induced by TNF-α via Modulation of the PI3K/Akt and NF-κB Signalling Pathways

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms20010036

    TAS suppressed TNF-α-induced apoptosis . HUVECs were preincubated with TAS (5 μg/mL) for 2 h, followed by treatment with TNF-α (50 ng/mL) for 24 h. HUVEC apoptosis was assessed by ( A ) TUNEL staining and ( B ) annexin V/propidium iodide (PI) staining. ( C ) Quantitative analysis of the ratio of TUNEL-positive cells. ( D ) Quantitative analysis of the ratio of apoptotic cells. ( E ) Cells were preincubated with TAS (5 μg/mL) for 2 h, followed by treatment with TNF-α for 24 h. Caspase-3 activity was examined with a fluorescent labelling kit using a microplate reader. The data are expressed as the mean ± SD of three independent experiments. ## p
    Figure Legend Snippet: TAS suppressed TNF-α-induced apoptosis . HUVECs were preincubated with TAS (5 μg/mL) for 2 h, followed by treatment with TNF-α (50 ng/mL) for 24 h. HUVEC apoptosis was assessed by ( A ) TUNEL staining and ( B ) annexin V/propidium iodide (PI) staining. ( C ) Quantitative analysis of the ratio of TUNEL-positive cells. ( D ) Quantitative analysis of the ratio of apoptotic cells. ( E ) Cells were preincubated with TAS (5 μg/mL) for 2 h, followed by treatment with TNF-α for 24 h. Caspase-3 activity was examined with a fluorescent labelling kit using a microplate reader. The data are expressed as the mean ± SD of three independent experiments. ## p

    Techniques Used: TUNEL Assay, Staining, Activity Assay

    Related Articles

    Western Blot:

    Article Title: Simvastatin inhibits the apoptosis of hippocampal cells in a mouse model of Alzheimer's disease
    Article Snippet: .. For western blot analysis, primary antibodies anti-bax (1:1,000; ab32503), anti-Bcl-2 (1:1,000; ab194583), anti-caspase-3 (1:1,000; ab13847), anti-caspase-9 (1:1,000; ab18571), anti-p38 (1:1,000; ab31828), anti-ERK (1:1,000; ab176660), anti β-actin (1:1,000; ab8226; all Abcam; Cambridge, UK) were added to PVDF membranes (EMD Millipore) and incubated at 4°C overnight. .. Membranes were blocked in 5% skimmed milk for 1 h at 37°C and then incubated with HRP-conjugated goat anti-rabbit IgG mAb (PV-6001; ZSGB-BIO) for 24 h at 4°C.

    Incubation:

    Article Title: Simvastatin inhibits the apoptosis of hippocampal cells in a mouse model of Alzheimer's disease
    Article Snippet: .. For western blot analysis, primary antibodies anti-bax (1:1,000; ab32503), anti-Bcl-2 (1:1,000; ab194583), anti-caspase-3 (1:1,000; ab13847), anti-caspase-9 (1:1,000; ab18571), anti-p38 (1:1,000; ab31828), anti-ERK (1:1,000; ab176660), anti β-actin (1:1,000; ab8226; all Abcam; Cambridge, UK) were added to PVDF membranes (EMD Millipore) and incubated at 4°C overnight. .. Membranes were blocked in 5% skimmed milk for 1 h at 37°C and then incubated with HRP-conjugated goat anti-rabbit IgG mAb (PV-6001; ZSGB-BIO) for 24 h at 4°C.

    Article Title: Therapeutic effects of simvastatin combined with kallistatin treatment for pediatric burn patients with sepsis
    Article Snippet: Protein samples (10 µg) were separated on 12.5% SDS-PAGE and transferred onto polyvinylidene difluoride membranes (Millipore, Massachusetts, USA). .. Membrane were blocked with 5% skimmed milk for 1 h at 37°C and then incubated with the following rabbit anti-human primary antibodies: B-cell lymphoma 2 (Bcl-2; 1:500; cat. no., ab692), P53 (1:500; cat. no., ab26), Bcl-2-associated X protein (1:500; cat. no., ab22048), caspase-3 (1:500, cat. no., ab13847) and β-actin (1:500; cat. no., ab8227; all purchased from Abcam, Shanghai, China) for 12 h at 4°C. .. Samples were then incubated with horseradish peroxidase (HRP)-conjugated anti-rabbit IgG antibody (1:5,000, PV-6001, ZSGB-BIO, Beijing, China) for 24 h at 4°C.

    Article Title: Traditional Chinese Medicine Formulation Huangqi Jianzhong Tang Improves Cardiac Function after Myocardial Infarction in Rats
    Article Snippet: The membranes were blocked in 5% nonfat milk in PBS and 0.1% Tween-20 at room temperature. .. The blots were then incubated with primary antibody: anti-Bcl-2 antibody (1:1000, ImmunoWay Biotech, Inc., Shanghai, China), anti-Bax antibody (1:1000, Abcam, Inc., Shanghai, China), anti-Caspase-3 antibody (1:1000, Abcam, Inc., Shanghai, China), or anti-GAPDH (Santa Cruz Biotech, Inc., Shanghai, China) for 2 h at room temperature or at 4°C overnight. ..

    Staining:

    Article Title: Potassium bromate-induced kidney damage in rats and the effect of gum acacia thereon
    Article Snippet: Briefly, the kidney (4 µm sections) were cut and stained with three stains: Hematoxylin and Eosin (H & E), Masson Trichrome and Sirius Red stains. .. Apoptotic cells were stained by anti-caspase 3 antibody using (1:100; Abcam, Ab4051), as previously reported [ ]. ..

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  • 97
    Abcam anti caspase 3 antibody
    a. <t>Caspase-3</t> immunostaining (control group). The control group caspase-3 results showed that caspase-3 expression was negative in germinal epithelial cells, granular cells of preantral and antral follicles, and endothelial cells. However, it was positive in the stromal cells around the follicle. b. Caspase-3 immunostaining (ischemia group). The positive expression of caspase-3 was observed in degenerated granular cells of preantral and antral follicles, luteal cells of the corpus luteum, and many inflammatory cells in the stromal region ( red arrow ). c. Caspase-3 immunostaining (ischemia-reperfusion group). Caspase-3 was positively expressed in granular cells in mature antral follicles and inflammatory cells in the stromal region ( red arrow ). d. Caspase-3 immunostaining (ischemia-reperfusion + RA group). Caspase-3 expression was negative in the preantral follicle cells and granular cells around the antral follicle ( red arrow ), whereas it was positive in some stromal cells and corpus luteum cells. Scale bar = 50 μm.
    Anti Caspase 3 Antibody, supplied by Abcam, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti caspase 3 antibody/product/Abcam
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti caspase 3 antibody - by Bioz Stars, 2021-06
    97/100 stars
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    99
    Abcam anti cleaved caspase 3
    Effect of miR-1 silencing on glucose-induced apoptosis. (A) Effect of miR-1 silencing on glucose-induced mitochondrial dysfunction in H9C2 cells. (B) Quantification of fluorescence intensity. (C) The expression of apoptosis-associated proteins, including Bcl-2, Bax, total/cleaved <t>caspase-3,</t> total/cleaved caspase-9 and total/cleaved PARP in H9C2 cells. (D) Quantitative analysis of western blot analysis results. **P
    Anti Cleaved Caspase 3, supplied by Abcam, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti cleaved caspase 3/product/Abcam
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti cleaved caspase 3 - by Bioz Stars, 2021-06
    99/100 stars
      Buy from Supplier


    Image Search Results


    a. Caspase-3 immunostaining (control group). The control group caspase-3 results showed that caspase-3 expression was negative in germinal epithelial cells, granular cells of preantral and antral follicles, and endothelial cells. However, it was positive in the stromal cells around the follicle. b. Caspase-3 immunostaining (ischemia group). The positive expression of caspase-3 was observed in degenerated granular cells of preantral and antral follicles, luteal cells of the corpus luteum, and many inflammatory cells in the stromal region ( red arrow ). c. Caspase-3 immunostaining (ischemia-reperfusion group). Caspase-3 was positively expressed in granular cells in mature antral follicles and inflammatory cells in the stromal region ( red arrow ). d. Caspase-3 immunostaining (ischemia-reperfusion + RA group). Caspase-3 expression was negative in the preantral follicle cells and granular cells around the antral follicle ( red arrow ), whereas it was positive in some stromal cells and corpus luteum cells. Scale bar = 50 μm.

    Journal: Acta Cirúrgica Brasileira

    Article Title: Investigation of the role of rosmarinic acid treatment in regulating inflammation, cell damage, and angiogenesis in rat ovarian torsion and detorsion models 1

    doi: 10.1590/s0102-865020200030000004

    Figure Lengend Snippet: a. Caspase-3 immunostaining (control group). The control group caspase-3 results showed that caspase-3 expression was negative in germinal epithelial cells, granular cells of preantral and antral follicles, and endothelial cells. However, it was positive in the stromal cells around the follicle. b. Caspase-3 immunostaining (ischemia group). The positive expression of caspase-3 was observed in degenerated granular cells of preantral and antral follicles, luteal cells of the corpus luteum, and many inflammatory cells in the stromal region ( red arrow ). c. Caspase-3 immunostaining (ischemia-reperfusion group). Caspase-3 was positively expressed in granular cells in mature antral follicles and inflammatory cells in the stromal region ( red arrow ). d. Caspase-3 immunostaining (ischemia-reperfusion + RA group). Caspase-3 expression was negative in the preantral follicle cells and granular cells around the antral follicle ( red arrow ), whereas it was positive in some stromal cells and corpus luteum cells. Scale bar = 50 μm.

    Article Snippet: The sections were washed three times for 5 min in PBS and were incubated with hydrogen peroxide (catalogue # TA-015-HP, Thermo Fisher Scientific, US) for 20 min. Ultra V block (TA-125-UB, Thermo Fisher Scientific, US) was applied to the sections for 8 min prior to the addition of the primary antibodies, which were left on overnight in VEGF antibody (catalogue # ab1316, 1:100), TNF-α antibody (catalogue # ab6671, 1:100), and caspase-3 antibody (catalogue # ab4051, 1:100), all from Abcam, US.

    Techniques: Immunostaining, Expressing

    Sulforaphane synergizes with cisplatin in suppressing cancer cell proliferation and promoting apoptosis of ovarian cancer cells. (A) Synergism between sulforaphane and cisplatin. A2780 cells were treated with sulforaphane and cisplatin at the indicated concentrations. After 24 h, WST-1 reagent was administrated to the culture medium and then incubated for 1 h. The WST-1 activities were determined at 440 nm. (B) Colony formation assay. Subconfluent A2780 cells were seeded in plates and treated with sulforaphane and cisplatin at the indicated concentrations. After 48 h, the cancer cells were then fixed and stained with crystal violet for the colony formation assay. (C) Colony-forming ability of ovarian cancer cells following sulforaphane and cisplatin treatment was quantified for optical absorbance. (D) Western blot assays with (E) quantification were performed to determine protein levels of Bcl-2, Caspase-3, P53, and Cyclin-D1 in A2780 cells treated with or without sulforaphane and cisplatin. The values are presented as the mean ± standard deviation (n=8-10) of the samples. + P

    Journal: International Journal of Molecular Medicine

    Article Title: Sulforaphane regulates apoptosis- and proliferation-related signaling pathways and synergizes with cisplatin to suppress human ovarian cancer

    doi: 10.3892/ijmm.2018.3860

    Figure Lengend Snippet: Sulforaphane synergizes with cisplatin in suppressing cancer cell proliferation and promoting apoptosis of ovarian cancer cells. (A) Synergism between sulforaphane and cisplatin. A2780 cells were treated with sulforaphane and cisplatin at the indicated concentrations. After 24 h, WST-1 reagent was administrated to the culture medium and then incubated for 1 h. The WST-1 activities were determined at 440 nm. (B) Colony formation assay. Subconfluent A2780 cells were seeded in plates and treated with sulforaphane and cisplatin at the indicated concentrations. After 48 h, the cancer cells were then fixed and stained with crystal violet for the colony formation assay. (C) Colony-forming ability of ovarian cancer cells following sulforaphane and cisplatin treatment was quantified for optical absorbance. (D) Western blot assays with (E) quantification were performed to determine protein levels of Bcl-2, Caspase-3, P53, and Cyclin-D1 in A2780 cells treated with or without sulforaphane and cisplatin. The values are presented as the mean ± standard deviation (n=8-10) of the samples. + P

    Article Snippet: The primary antibodies used were: Rabbit anti-P53 (cat. no. 2527), anti-Bcl-2 (cat. no. 3498), anti-phosphorylated nuclear factor-κB (p-NF-κB; cat. no. 3033), anti-cMyc (cat. no. 5605), anti-AKT (cat. no. 4685) and anti-p-AKT (cat. no. 4060) from Cell Signaling Technology, Inc.; rabbit anti-Bcl-2-associated X protein (Bax; cat. no. ab32503), anti-NF-κB (cat. no. ab16502), anti-Caspase-3 (cat. no. 13847), anti-Cyclin-D1 (cat. no. 134175) and mouse anti-P27 (cat. no. 193379) from Abcam (Cambridge, MA, USA); and rabbit anti-cytochrome c (cyto-c; cat. no. sc-13561) and anti-GAPDH (cat. no. sc-47724) from Santa Cruz Biotechnology, Inc. (Dallas, TX, USA).

    Techniques: Incubation, Colony Assay, Staining, Western Blot, Standard Deviation

    Sulforaphane inhibits multiple cancer development-associated signaling pathways in A2780 cells. (A) Effect of sulforaphane on intracellular levels of Her2 through immunofluorescence assays (magnification, ×100). (B) Western blot assays were performed to examine the effect of sulforaphane on the 10 cancer-associated signaling pathway reporter activities, including Bcl-2, Bax, Cyto-c, Caspase-3, p-AKT, p-NF-κB, P53, P27, cyclin-D1 and cMyc. The values are presented as the mean ± standard deviation (n=8-10) of the samples. + P

    Journal: International Journal of Molecular Medicine

    Article Title: Sulforaphane regulates apoptosis- and proliferation-related signaling pathways and synergizes with cisplatin to suppress human ovarian cancer

    doi: 10.3892/ijmm.2018.3860

    Figure Lengend Snippet: Sulforaphane inhibits multiple cancer development-associated signaling pathways in A2780 cells. (A) Effect of sulforaphane on intracellular levels of Her2 through immunofluorescence assays (magnification, ×100). (B) Western blot assays were performed to examine the effect of sulforaphane on the 10 cancer-associated signaling pathway reporter activities, including Bcl-2, Bax, Cyto-c, Caspase-3, p-AKT, p-NF-κB, P53, P27, cyclin-D1 and cMyc. The values are presented as the mean ± standard deviation (n=8-10) of the samples. + P

    Article Snippet: The primary antibodies used were: Rabbit anti-P53 (cat. no. 2527), anti-Bcl-2 (cat. no. 3498), anti-phosphorylated nuclear factor-κB (p-NF-κB; cat. no. 3033), anti-cMyc (cat. no. 5605), anti-AKT (cat. no. 4685) and anti-p-AKT (cat. no. 4060) from Cell Signaling Technology, Inc.; rabbit anti-Bcl-2-associated X protein (Bax; cat. no. ab32503), anti-NF-κB (cat. no. ab16502), anti-Caspase-3 (cat. no. 13847), anti-Cyclin-D1 (cat. no. 134175) and mouse anti-P27 (cat. no. 193379) from Abcam (Cambridge, MA, USA); and rabbit anti-cytochrome c (cyto-c; cat. no. sc-13561) and anti-GAPDH (cat. no. sc-47724) from Santa Cruz Biotechnology, Inc. (Dallas, TX, USA).

    Techniques: Immunofluorescence, Western Blot, Standard Deviation

    Effect of miR-1 silencing on glucose-induced apoptosis. (A) Effect of miR-1 silencing on glucose-induced mitochondrial dysfunction in H9C2 cells. (B) Quantification of fluorescence intensity. (C) The expression of apoptosis-associated proteins, including Bcl-2, Bax, total/cleaved caspase-3, total/cleaved caspase-9 and total/cleaved PARP in H9C2 cells. (D) Quantitative analysis of western blot analysis results. **P

    Journal: Experimental and Therapeutic Medicine

    Article Title: Downregulation of microRNA-1 attenuates glucose-induced apoptosis by regulating the liver X receptor α in cardiomyocytes

    doi: 10.3892/etm.2018.6388

    Figure Lengend Snippet: Effect of miR-1 silencing on glucose-induced apoptosis. (A) Effect of miR-1 silencing on glucose-induced mitochondrial dysfunction in H9C2 cells. (B) Quantification of fluorescence intensity. (C) The expression of apoptosis-associated proteins, including Bcl-2, Bax, total/cleaved caspase-3, total/cleaved caspase-9 and total/cleaved PARP in H9C2 cells. (D) Quantitative analysis of western blot analysis results. **P

    Article Snippet: Membranes were incubated with primary antibodies overnight at 4°C and subsequently incubated with horseradish peroxidase-conjugated goat anti-rabbit/mouse immunoglobulin G secondary antibodies (1:5,000; Beyotime Institute of Biotechnology) at room temperature for 45 min. Anti-cleaved-poly (adenosine diphosphate-ribose) polymerase (PARP; cat. no. ab32561; 1:1,000), anti-cleaved-caspase-3 (cat. no. ab2302; 1:1,000), anti-cleaved-caspase-9 (cat. no. ab25758; 1:1,000) and anti-LXRα (cat. no. ab3585; 1:300) primary antibodies were obtained from Abcam (Cambridge, MA, USA).

    Techniques: Fluorescence, Expressing, Western Blot

    Effects of miR-10a on the intrinsic apoptosis pathway. SH-SY5Y cells were transfected with or without A30P α-syn and co-transfected with either miR-NC or miR-10a mimics. Protein expression levels of BCL2, BAX, and cleaved caspase-3 were detected by Western blotting. Data are presented as the mean ± standard deviation (n=3). * P

    Journal: International Journal of Clinical and Experimental Pathology

    Article Title: MicroRNA-10a inhibits A30P α-synuclein aggregation and toxicity by targeting proapoptotic protein BCL2L11

    doi:

    Figure Lengend Snippet: Effects of miR-10a on the intrinsic apoptosis pathway. SH-SY5Y cells were transfected with or without A30P α-syn and co-transfected with either miR-NC or miR-10a mimics. Protein expression levels of BCL2, BAX, and cleaved caspase-3 were detected by Western blotting. Data are presented as the mean ± standard deviation (n=3). * P

    Article Snippet: The anti-cleaved caspase-3 antibody (1:500; catalog no. ab49822) was obtained from Abcam (Cambridge, UK).

    Techniques: Transfection, Expressing, Western Blot, Standard Deviation

    Effect of BCL2L11 knockdown on A30P α-syn aggregation and toxicity in SH-SY5Y cells. (A) The inhibitory effect of si-BCL2L11 on the expression of BCL2L11 proteins was confirmed by Western blotting. (B) Cell viability and (C) cytotoxicity were evaluated 48 h post-transfection with A30P α-syn and si-BCL2L11 by MTT and LDH assays, respectively. (D) α-syn-positive aggregates in every 100 cells were counted. (E) Percentage of cells with α-syn aggregates. (F) Western blotting was conducted to measure the expression levels of BCL2, BAX, and cleaved caspase-3 in SH-SY5Y cells co-transfected with A30P α-syn and si-BCL2L11. Data are presented as the mean ± standard deviation (n=3); * P

    Journal: International Journal of Clinical and Experimental Pathology

    Article Title: MicroRNA-10a inhibits A30P α-synuclein aggregation and toxicity by targeting proapoptotic protein BCL2L11

    doi:

    Figure Lengend Snippet: Effect of BCL2L11 knockdown on A30P α-syn aggregation and toxicity in SH-SY5Y cells. (A) The inhibitory effect of si-BCL2L11 on the expression of BCL2L11 proteins was confirmed by Western blotting. (B) Cell viability and (C) cytotoxicity were evaluated 48 h post-transfection with A30P α-syn and si-BCL2L11 by MTT and LDH assays, respectively. (D) α-syn-positive aggregates in every 100 cells were counted. (E) Percentage of cells with α-syn aggregates. (F) Western blotting was conducted to measure the expression levels of BCL2, BAX, and cleaved caspase-3 in SH-SY5Y cells co-transfected with A30P α-syn and si-BCL2L11. Data are presented as the mean ± standard deviation (n=3); * P

    Article Snippet: The anti-cleaved caspase-3 antibody (1:500; catalog no. ab49822) was obtained from Abcam (Cambridge, UK).

    Techniques: Expressing, Western Blot, Transfection, MTT Assay, Standard Deviation