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Santa Cruz Biotechnology anti car phycoerythrin pe antibody
Up-regulation of cell-surface <t>CAR</t> levels upon ZEB1 knockdown coincides with increased adenoviral infectability . A and B . Cell-surface CAR levels following ZEB1 knockdown were measured by flow <t>cytometry</t> on PANC-1 cells treated with TGF-β1 ( A ), or on MDA-MB-231 cells ( B ). Error bars represent standard deviations (biological duplicates). C and D . Ad-GFP uptake following ZEB1 knockdown was determined by flow cytometry measuring GFP levels ( C ), or by real-time PCR for virus copy number ( D ). Error bars represent standard deviations. A-D . CAR and GFP signals are expressed as geometric means of the fluorescence signal intensities normalized to the untreated controls (A-C), virus copy numbers as relative adenovirus fiber levels (D). Abbreviations: UT, untransfected; Ctrl #1, siControl ON-TARGETplus Non-targeting siRNA #1 (Dharmacon); Ctrl #2, firefly luciferase-targeting siRNA; ZEB1 siRNA #1/#2, ZEB1-targeting siRNAs. Ctrl #2 and ZEB1 siRNA sequences are provided in Additional file 1 (Table S3). p
Anti Car Phycoerythrin Pe Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 85/100, based on 313 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti car phycoerythrin pe antibody/product/Santa Cruz Biotechnology
Average 85 stars, based on 313 article reviews
Price from $9.99 to $1999.99
anti car phycoerythrin pe antibody - by Bioz Stars, 2020-07
85/100 stars

Images

1) Product Images from "ZEB1 limits adenoviral infectability by transcriptionally repressing the Coxsackie virus and Adenovirus Receptor"

Article Title: ZEB1 limits adenoviral infectability by transcriptionally repressing the Coxsackie virus and Adenovirus Receptor

Journal: Molecular Cancer

doi: 10.1186/1476-4598-10-91

Up-regulation of cell-surface CAR levels upon ZEB1 knockdown coincides with increased adenoviral infectability . A and B . Cell-surface CAR levels following ZEB1 knockdown were measured by flow cytometry on PANC-1 cells treated with TGF-β1 ( A ), or on MDA-MB-231 cells ( B ). Error bars represent standard deviations (biological duplicates). C and D . Ad-GFP uptake following ZEB1 knockdown was determined by flow cytometry measuring GFP levels ( C ), or by real-time PCR for virus copy number ( D ). Error bars represent standard deviations. A-D . CAR and GFP signals are expressed as geometric means of the fluorescence signal intensities normalized to the untreated controls (A-C), virus copy numbers as relative adenovirus fiber levels (D). Abbreviations: UT, untransfected; Ctrl #1, siControl ON-TARGETplus Non-targeting siRNA #1 (Dharmacon); Ctrl #2, firefly luciferase-targeting siRNA; ZEB1 siRNA #1/#2, ZEB1-targeting siRNAs. Ctrl #2 and ZEB1 siRNA sequences are provided in Additional file 1 (Table S3). p
Figure Legend Snippet: Up-regulation of cell-surface CAR levels upon ZEB1 knockdown coincides with increased adenoviral infectability . A and B . Cell-surface CAR levels following ZEB1 knockdown were measured by flow cytometry on PANC-1 cells treated with TGF-β1 ( A ), or on MDA-MB-231 cells ( B ). Error bars represent standard deviations (biological duplicates). C and D . Ad-GFP uptake following ZEB1 knockdown was determined by flow cytometry measuring GFP levels ( C ), or by real-time PCR for virus copy number ( D ). Error bars represent standard deviations. A-D . CAR and GFP signals are expressed as geometric means of the fluorescence signal intensities normalized to the untreated controls (A-C), virus copy numbers as relative adenovirus fiber levels (D). Abbreviations: UT, untransfected; Ctrl #1, siControl ON-TARGETplus Non-targeting siRNA #1 (Dharmacon); Ctrl #2, firefly luciferase-targeting siRNA; ZEB1 siRNA #1/#2, ZEB1-targeting siRNAs. Ctrl #2 and ZEB1 siRNA sequences are provided in Additional file 1 (Table S3). p

Techniques Used: Flow Cytometry, Cytometry, Multiple Displacement Amplification, Real-time Polymerase Chain Reaction, Fluorescence, Luciferase

2) Product Images from "Coxsackievirus and adenovirus receptor mediates the responses of endothelial cells to fluid shear stress"

Article Title: Coxsackievirus and adenovirus receptor mediates the responses of endothelial cells to fluid shear stress

Journal: Experimental & Molecular Medicine

doi: 10.1038/s12276-019-0347-7

Schematic of the proposed role of CAR in endothelial mechanotransduction signaling under FSS. Endothelial CAR expression is regulated by FSS. Disturbed flow increases, whereas laminar shear stress decreases, CAR expression in ECs; these changes in CAR expression are regulated through the KLF2-AP-1 axis. Disturbed flow-induced CAR inhibits the phosphorylation of PECAM-1, leading to the suppression of endothelial mechanotransduction signaling through the phosphorylation of Src, PI3K, Akt, and eNOS. Therefore, CAR modulates mechanotransduction in ECs by blocking PECAM-1 phosphorylation, which influences endothelial function and vascular pathophysiology.
Figure Legend Snippet: Schematic of the proposed role of CAR in endothelial mechanotransduction signaling under FSS. Endothelial CAR expression is regulated by FSS. Disturbed flow increases, whereas laminar shear stress decreases, CAR expression in ECs; these changes in CAR expression are regulated through the KLF2-AP-1 axis. Disturbed flow-induced CAR inhibits the phosphorylation of PECAM-1, leading to the suppression of endothelial mechanotransduction signaling through the phosphorylation of Src, PI3K, Akt, and eNOS. Therefore, CAR modulates mechanotransduction in ECs by blocking PECAM-1 phosphorylation, which influences endothelial function and vascular pathophysiology.

Techniques Used: Expressing, Flow Cytometry, Blocking Assay

CAR deletion protects the proatherogenic endothelium under disturbed flow. Wild-type (WT) C57BL/6 or EC-specific CAR KO mice were partially ligated in the LCA. a Twenty-four hours after LCA ligation, the mice were euthanized, and EC-enriched RNA was extracted from both carotid arteries. EC-enriched RNA was confirmed by assessing an endothelial-specific marker (PECAM-1), a smooth muscle cell-specific marker (α-smooth muscle actin [α-SMA]), and β-actin (internal control). HUVECs were used as a positive control for PECAM-1 and a negative control for α-SMA. Human vascular smooth muscle cells (VSMCs) were used as a positive control for α-SMA and a negative control for PECAM-1. The knockout of endothelial CAR in EC-specific CAR KO mice was confirmed by RT-PCR. Representative images are shown ( n = 5). b Levels of mRNAs encoding eNOS, VCAM-1, and ICAM-1 in EC-enriched RNA were determined by real-time PCR ( n = 5; * P
Figure Legend Snippet: CAR deletion protects the proatherogenic endothelium under disturbed flow. Wild-type (WT) C57BL/6 or EC-specific CAR KO mice were partially ligated in the LCA. a Twenty-four hours after LCA ligation, the mice were euthanized, and EC-enriched RNA was extracted from both carotid arteries. EC-enriched RNA was confirmed by assessing an endothelial-specific marker (PECAM-1), a smooth muscle cell-specific marker (α-smooth muscle actin [α-SMA]), and β-actin (internal control). HUVECs were used as a positive control for PECAM-1 and a negative control for α-SMA. Human vascular smooth muscle cells (VSMCs) were used as a positive control for α-SMA and a negative control for PECAM-1. The knockout of endothelial CAR in EC-specific CAR KO mice was confirmed by RT-PCR. Representative images are shown ( n = 5). b Levels of mRNAs encoding eNOS, VCAM-1, and ICAM-1 in EC-enriched RNA were determined by real-time PCR ( n = 5; * P

Techniques Used: Flow Cytometry, Mouse Assay, Ligation, Marker, Positive Control, Negative Control, Knock-Out, Reverse Transcription Polymerase Chain Reaction, Real-time Polymerase Chain Reaction

3) Product Images from "Coxsackievirus and adenovirus receptor mediates the responses of endothelial cells to fluid shear stress"

Article Title: Coxsackievirus and adenovirus receptor mediates the responses of endothelial cells to fluid shear stress

Journal: Experimental & Molecular Medicine

doi: 10.1038/s12276-019-0347-7

Schematic of the proposed role of CAR in endothelial mechanotransduction signaling under FSS. Endothelial CAR expression is regulated by FSS. Disturbed flow increases, whereas laminar shear stress decreases, CAR expression in ECs; these changes in CAR expression are regulated through the KLF2-AP-1 axis. Disturbed flow-induced CAR inhibits the phosphorylation of PECAM-1, leading to the suppression of endothelial mechanotransduction signaling through the phosphorylation of Src, PI3K, Akt, and eNOS. Therefore, CAR modulates mechanotransduction in ECs by blocking PECAM-1 phosphorylation, which influences endothelial function and vascular pathophysiology.
Figure Legend Snippet: Schematic of the proposed role of CAR in endothelial mechanotransduction signaling under FSS. Endothelial CAR expression is regulated by FSS. Disturbed flow increases, whereas laminar shear stress decreases, CAR expression in ECs; these changes in CAR expression are regulated through the KLF2-AP-1 axis. Disturbed flow-induced CAR inhibits the phosphorylation of PECAM-1, leading to the suppression of endothelial mechanotransduction signaling through the phosphorylation of Src, PI3K, Akt, and eNOS. Therefore, CAR modulates mechanotransduction in ECs by blocking PECAM-1 phosphorylation, which influences endothelial function and vascular pathophysiology.

Techniques Used: Expressing, Flow Cytometry, Blocking Assay

CAR deletion protects the proatherogenic endothelium under disturbed flow. Wild-type (WT) C57BL/6 or EC-specific CAR KO mice were partially ligated in the LCA. a Twenty-four hours after LCA ligation, the mice were euthanized, and EC-enriched RNA was extracted from both carotid arteries. EC-enriched RNA was confirmed by assessing an endothelial-specific marker (PECAM-1), a smooth muscle cell-specific marker (α-smooth muscle actin [α-SMA]), and β-actin (internal control). HUVECs were used as a positive control for PECAM-1 and a negative control for α-SMA. Human vascular smooth muscle cells (VSMCs) were used as a positive control for α-SMA and a negative control for PECAM-1. The knockout of endothelial CAR in EC-specific CAR KO mice was confirmed by RT-PCR. Representative images are shown ( n = 5). b Levels of mRNAs encoding eNOS, VCAM-1, and ICAM-1 in EC-enriched RNA were determined by real-time PCR ( n = 5; * P
Figure Legend Snippet: CAR deletion protects the proatherogenic endothelium under disturbed flow. Wild-type (WT) C57BL/6 or EC-specific CAR KO mice were partially ligated in the LCA. a Twenty-four hours after LCA ligation, the mice were euthanized, and EC-enriched RNA was extracted from both carotid arteries. EC-enriched RNA was confirmed by assessing an endothelial-specific marker (PECAM-1), a smooth muscle cell-specific marker (α-smooth muscle actin [α-SMA]), and β-actin (internal control). HUVECs were used as a positive control for PECAM-1 and a negative control for α-SMA. Human vascular smooth muscle cells (VSMCs) were used as a positive control for α-SMA and a negative control for PECAM-1. The knockout of endothelial CAR in EC-specific CAR KO mice was confirmed by RT-PCR. Representative images are shown ( n = 5). b Levels of mRNAs encoding eNOS, VCAM-1, and ICAM-1 in EC-enriched RNA were determined by real-time PCR ( n = 5; * P

Techniques Used: Flow Cytometry, Mouse Assay, Ligation, Marker, Positive Control, Negative Control, Knock-Out, Reverse Transcription Polymerase Chain Reaction, Real-time Polymerase Chain Reaction

4) Product Images from "Coxsackievirus and adenovirus receptor mediates the responses of endothelial cells to fluid shear stress"

Article Title: Coxsackievirus and adenovirus receptor mediates the responses of endothelial cells to fluid shear stress

Journal: Experimental & Molecular Medicine

doi: 10.1038/s12276-019-0347-7

Fluid shear stress regulates CAR expression in endothelial cells. HUVECs were cultured under static, LSS, or OSS conditions for the indicated periods of time. a Levels of mRNAs encoding CAR and GAPDH (internal control) were determined by RT-PCR. b CAR protein levels were measured by western blotting. Representative images are shown ( n = 5; compared with static conditions, * P
Figure Legend Snippet: Fluid shear stress regulates CAR expression in endothelial cells. HUVECs were cultured under static, LSS, or OSS conditions for the indicated periods of time. a Levels of mRNAs encoding CAR and GAPDH (internal control) were determined by RT-PCR. b CAR protein levels were measured by western blotting. Representative images are shown ( n = 5; compared with static conditions, * P

Techniques Used: Expressing, Cell Culture, Reverse Transcription Polymerase Chain Reaction, Western Blot

CAR knockdown inhibits disturbed flow-induced inflammatory responses in endothelial cells. HUVECs transfected with siRNA against CAR were exposed to FSS for 6 h ( b ) or 24 h ( a ). a Cytosolic and nuclear fractions were separated after 24 h of FSS. The extent of IκB phosphorylation and nuclear translocation of NF-κB were determined by western blotting with antibodies against p-IκB, NF-κB p65, GAPDH, and Lamin A/C. GAPDH and Lamin A/C were used as loading controls for the cytosolic and nuclear fractions, respectively. Representative images are shown ( n = 5; * P
Figure Legend Snippet: CAR knockdown inhibits disturbed flow-induced inflammatory responses in endothelial cells. HUVECs transfected with siRNA against CAR were exposed to FSS for 6 h ( b ) or 24 h ( a ). a Cytosolic and nuclear fractions were separated after 24 h of FSS. The extent of IκB phosphorylation and nuclear translocation of NF-κB were determined by western blotting with antibodies against p-IκB, NF-κB p65, GAPDH, and Lamin A/C. GAPDH and Lamin A/C were used as loading controls for the cytosolic and nuclear fractions, respectively. Representative images are shown ( n = 5; * P

Techniques Used: Flow Cytometry, Transfection, Translocation Assay, Western Blot

5) Product Images from "Coxsackievirus and adenovirus receptor mediates the responses of endothelial cells to fluid shear stress"

Article Title: Coxsackievirus and adenovirus receptor mediates the responses of endothelial cells to fluid shear stress

Journal: Experimental & Molecular Medicine

doi: 10.1038/s12276-019-0347-7

Fluid shear stress regulates CAR expression in endothelial cells. HUVECs were cultured under static, LSS, or OSS conditions for the indicated periods of time. a Levels of mRNAs encoding CAR and GAPDH (internal control) were determined by RT-PCR. b CAR protein levels were measured by western blotting. Representative images are shown ( n = 5; compared with static conditions, * P
Figure Legend Snippet: Fluid shear stress regulates CAR expression in endothelial cells. HUVECs were cultured under static, LSS, or OSS conditions for the indicated periods of time. a Levels of mRNAs encoding CAR and GAPDH (internal control) were determined by RT-PCR. b CAR protein levels were measured by western blotting. Representative images are shown ( n = 5; compared with static conditions, * P

Techniques Used: Expressing, Cell Culture, Reverse Transcription Polymerase Chain Reaction, Western Blot

CAR knockdown inhibits disturbed flow-induced inflammatory responses in endothelial cells. HUVECs transfected with siRNA against CAR were exposed to FSS for 6 h ( b ) or 24 h ( a ). a Cytosolic and nuclear fractions were separated after 24 h of FSS. The extent of IκB phosphorylation and nuclear translocation of NF-κB were determined by western blotting with antibodies against p-IκB, NF-κB p65, GAPDH, and Lamin A/C. GAPDH and Lamin A/C were used as loading controls for the cytosolic and nuclear fractions, respectively. Representative images are shown ( n = 5; * P
Figure Legend Snippet: CAR knockdown inhibits disturbed flow-induced inflammatory responses in endothelial cells. HUVECs transfected with siRNA against CAR were exposed to FSS for 6 h ( b ) or 24 h ( a ). a Cytosolic and nuclear fractions were separated after 24 h of FSS. The extent of IκB phosphorylation and nuclear translocation of NF-κB were determined by western blotting with antibodies against p-IκB, NF-κB p65, GAPDH, and Lamin A/C. GAPDH and Lamin A/C were used as loading controls for the cytosolic and nuclear fractions, respectively. Representative images are shown ( n = 5; * P

Techniques Used: Flow Cytometry, Transfection, Translocation Assay, Western Blot

6) Product Images from "ZEB1 limits adenoviral infectability by transcriptionally repressing the Coxsackie virus and Adenovirus Receptor"

Article Title: ZEB1 limits adenoviral infectability by transcriptionally repressing the Coxsackie virus and Adenovirus Receptor

Journal: Molecular Cancer

doi: 10.1186/1476-4598-10-91

Up-regulation of cell-surface CAR levels upon ZEB1 knockdown coincides with increased adenoviral infectability . A and B . Cell-surface CAR levels following ZEB1 knockdown were measured by flow cytometry on PANC-1 cells treated with TGF-β1 ( A ), or on MDA-MB-231 cells ( B ). Error bars represent standard deviations (biological duplicates). C and D . Ad-GFP uptake following ZEB1 knockdown was determined by flow cytometry measuring GFP levels ( C ), or by real-time PCR for virus copy number ( D ). Error bars represent standard deviations. A-D . CAR and GFP signals are expressed as geometric means of the fluorescence signal intensities normalized to the untreated controls (A-C), virus copy numbers as relative adenovirus fiber levels (D). Abbreviations: UT, untransfected; Ctrl #1, siControl ON-TARGETplus Non-targeting siRNA #1 (Dharmacon); Ctrl #2, firefly luciferase-targeting siRNA; ZEB1 siRNA #1/#2, ZEB1-targeting siRNAs. Ctrl #2 and ZEB1 siRNA sequences are provided in Additional file 1 (Table S3). p
Figure Legend Snippet: Up-regulation of cell-surface CAR levels upon ZEB1 knockdown coincides with increased adenoviral infectability . A and B . Cell-surface CAR levels following ZEB1 knockdown were measured by flow cytometry on PANC-1 cells treated with TGF-β1 ( A ), or on MDA-MB-231 cells ( B ). Error bars represent standard deviations (biological duplicates). C and D . Ad-GFP uptake following ZEB1 knockdown was determined by flow cytometry measuring GFP levels ( C ), or by real-time PCR for virus copy number ( D ). Error bars represent standard deviations. A-D . CAR and GFP signals are expressed as geometric means of the fluorescence signal intensities normalized to the untreated controls (A-C), virus copy numbers as relative adenovirus fiber levels (D). Abbreviations: UT, untransfected; Ctrl #1, siControl ON-TARGETplus Non-targeting siRNA #1 (Dharmacon); Ctrl #2, firefly luciferase-targeting siRNA; ZEB1 siRNA #1/#2, ZEB1-targeting siRNAs. Ctrl #2 and ZEB1 siRNA sequences are provided in Additional file 1 (Table S3). p

Techniques Used: Flow Cytometry, Cytometry, Multiple Displacement Amplification, Real-time Polymerase Chain Reaction, Fluorescence, Luciferase

Related Articles

Staining:

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Article Snippet: .. HUVEC monolayers fixed with 4% formaldehyde and permeabilized with 0.1% Triton-X were incubated with a primary rabbit anti-CCHFV NP ( ) and/or mouse anti VE-cadherin (Santa Cruz Biotechnology Inc.) for 1 h. Slides stained with VE-cadherin were then incubated with anti-rabbit Texas red-conjugated and anti-mouse FITC-conjugated antibodies. .. DAPI (4′,6-diamidino-2-phenylindole) (Sigma, St. Louis, MO) was added to stain cell nuclei.

Article Title: Endomucin inhibits VEGF-induced endothelial cell migration, growth, and morphogenesis by modulating VEGFR2 signaling
Article Snippet: .. Retinas were stained with Alexa 488-labeled isolectin-B4 (Life Technologies), rat anti-EMCN (Santa Cruz Biotechnology), and rabbit anti-phospho-histone H3 (Millipore) while immunoblots were probed with antibodies against EMCN (Abcam), rabbit anti-GAPDH (Santa Cruz Biotechnology), phospho-Y951-VEGFR2, VEGFR2, phospho-p38-MAPK, p38-MAPK, phospho-ERK, ERK, phospho-FAK, and FAK (Cell Signaling). .. Retinal vessel development in vivo siRNAs were injected intravitreally under a dissecting microscope into P4 C57BL/6 J mice using a Hamilton syringe with a 33-gauge blunt-ended needle.

Incubation:

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Article Snippet: .. Membranes subsequently were incubated with mouse anti-CPT1A (1 : 1000, Abcam), rabbit anti-peroxisome proliferator-activated receptor α (PPARα ) (1 : 1000, Abcam), and rabbit anti-GAPDH (1 : 3000, Santa Cruz Biotechnology, Santa Cruz, CA, USA) antibodies at 4°C overnight. .. After the reaction, membranes were treated with a horseradish peroxidase- (HRP-) conjugated secondary antibody (anti-rabbit IgG [1 : 25000, Bio-Rad Laboratories] or anti-mouse IgG antibody [1 : 25000, Bio-Rad Laboratories]) for 1 hour at room temperature.

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Article Snippet: .. After blocking with 10% dried milk powder in 1x PBS membranes were probed with either rabbit anti-GAPDH (1:1000, Santa Cruz), rabbit anti-hnRNP H1 (1:1000, GeneTex) or rabbit anti-hnRNP K (1:1000, GeneTex) diluted in PBS plus 1% milk and incubated overnight at 4°C. .. After washing in PBS the appropriate HRP-conjugated secondary antibodies (Dako) diluted in PBS were added.

Article Title: Crimean-Congo Hemorrhagic Fever Virus Activates Endothelial Cells ▿
Article Snippet: .. HUVEC monolayers fixed with 4% formaldehyde and permeabilized with 0.1% Triton-X were incubated with a primary rabbit anti-CCHFV NP ( ) and/or mouse anti VE-cadherin (Santa Cruz Biotechnology Inc.) for 1 h. Slides stained with VE-cadherin were then incubated with anti-rabbit Texas red-conjugated and anti-mouse FITC-conjugated antibodies. .. DAPI (4′,6-diamidino-2-phenylindole) (Sigma, St. Louis, MO) was added to stain cell nuclei.

other:

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Article Snippet: Reagents The following antibodies were used: R & D Systems: goat anti-PDGFRα (AF1062); Proteintech: rabbit anti–human IFT20 (13615–1-AP), rabbit anti-IFT88 (13967-1-AP); Sigma-Aldrich: mouse anti–α-tubulin (T5168), mouse anti–acetylated α-tubulin (T6793), mouse anti-FLAG (M2; F1802), rabbit anti-FLAG (F7425); BD Biosciences: mouse anti-CDK1 (610038), mouse anti-p150Glued (610474), mouse anti-GM130 (610823); Enzo life Sciences: mouse anti-giantin (ALX-804-600-C100), mouse anti–mono- and polyubiquitin (FK2; BML-PW8810); Abcam: mouse anti–c-Cbl (Ab119954), rabbit anti–c-Cbl (Ab32027), mouse anti-GFP (Ab1218), chicken anti-GFP (Ab13970), rabbit anti–detyrosinated α-tubulin (Ab48389), rabbit anti-PDGFRα (Ab134123); Cell Signaling Technology: rabbit anti-Myc (2278), rabbit anti-GAPDH (2118), rabbit anti–Cbl-b (9498), rabbit anti-Akt (9272), rabbit anti–phosphorylated Akt (Ser473; 4060), rabbit anti-Erk1/2 (9102), rabbit anti–phosphorylated Erk1/2 (Thr202/Tyr204; 9101), rabbit anti–phosphorylated Rb (9308); Santa Cruz Biotechnology: rabbit anti–c-Cblsc-170), mouse anti–c-Cbl (sc-1651), mouse anti-GFP (sc-9996), rabbit anti-GFP (sc-8334), rabbit anti–phosphorylated PDGFRα (Tyr754; sc-12911R).

Blocking Assay:

Article Title: Regulation of Mcl-1 alternative splicing by hnRNP F, H1 and K in breast cancer cells
Article Snippet: .. After blocking with 10% dried milk powder in 1x PBS membranes were probed with either rabbit anti-GAPDH (1:1000, Santa Cruz), rabbit anti-hnRNP H1 (1:1000, GeneTex) or rabbit anti-hnRNP K (1:1000, GeneTex) diluted in PBS plus 1% milk and incubated overnight at 4°C. .. After washing in PBS the appropriate HRP-conjugated secondary antibodies (Dako) diluted in PBS were added.

Western Blot:

Article Title: Endomucin inhibits VEGF-induced endothelial cell migration, growth, and morphogenesis by modulating VEGFR2 signaling
Article Snippet: .. Retinas were stained with Alexa 488-labeled isolectin-B4 (Life Technologies), rat anti-EMCN (Santa Cruz Biotechnology), and rabbit anti-phospho-histone H3 (Millipore) while immunoblots were probed with antibodies against EMCN (Abcam), rabbit anti-GAPDH (Santa Cruz Biotechnology), phospho-Y951-VEGFR2, VEGFR2, phospho-p38-MAPK, p38-MAPK, phospho-ERK, ERK, phospho-FAK, and FAK (Cell Signaling). .. Retinal vessel development in vivo siRNAs were injected intravitreally under a dissecting microscope into P4 C57BL/6 J mice using a Hamilton syringe with a 33-gauge blunt-ended needle.

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    Santa Cruz Biotechnology anti car phycoerythrin pe antibody
    Up-regulation of cell-surface <t>CAR</t> levels upon ZEB1 knockdown coincides with increased adenoviral infectability . A and B . Cell-surface CAR levels following ZEB1 knockdown were measured by flow <t>cytometry</t> on PANC-1 cells treated with TGF-β1 ( A ), or on MDA-MB-231 cells ( B ). Error bars represent standard deviations (biological duplicates). C and D . Ad-GFP uptake following ZEB1 knockdown was determined by flow cytometry measuring GFP levels ( C ), or by real-time PCR for virus copy number ( D ). Error bars represent standard deviations. A-D . CAR and GFP signals are expressed as geometric means of the fluorescence signal intensities normalized to the untreated controls (A-C), virus copy numbers as relative adenovirus fiber levels (D). Abbreviations: UT, untransfected; Ctrl #1, siControl ON-TARGETplus Non-targeting siRNA #1 (Dharmacon); Ctrl #2, firefly luciferase-targeting siRNA; ZEB1 siRNA #1/#2, ZEB1-targeting siRNAs. Ctrl #2 and ZEB1 siRNA sequences are provided in Additional file 1 (Table S3). p
    Anti Car Phycoerythrin Pe Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti car phycoerythrin pe antibody/product/Santa Cruz Biotechnology
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti car phycoerythrin pe antibody - by Bioz Stars, 2020-07
    85/100 stars
      Buy from Supplier

    Image Search Results


    Up-regulation of cell-surface CAR levels upon ZEB1 knockdown coincides with increased adenoviral infectability . A and B . Cell-surface CAR levels following ZEB1 knockdown were measured by flow cytometry on PANC-1 cells treated with TGF-β1 ( A ), or on MDA-MB-231 cells ( B ). Error bars represent standard deviations (biological duplicates). C and D . Ad-GFP uptake following ZEB1 knockdown was determined by flow cytometry measuring GFP levels ( C ), or by real-time PCR for virus copy number ( D ). Error bars represent standard deviations. A-D . CAR and GFP signals are expressed as geometric means of the fluorescence signal intensities normalized to the untreated controls (A-C), virus copy numbers as relative adenovirus fiber levels (D). Abbreviations: UT, untransfected; Ctrl #1, siControl ON-TARGETplus Non-targeting siRNA #1 (Dharmacon); Ctrl #2, firefly luciferase-targeting siRNA; ZEB1 siRNA #1/#2, ZEB1-targeting siRNAs. Ctrl #2 and ZEB1 siRNA sequences are provided in Additional file 1 (Table S3). p

    Journal: Molecular Cancer

    Article Title: ZEB1 limits adenoviral infectability by transcriptionally repressing the Coxsackie virus and Adenovirus Receptor

    doi: 10.1186/1476-4598-10-91

    Figure Lengend Snippet: Up-regulation of cell-surface CAR levels upon ZEB1 knockdown coincides with increased adenoviral infectability . A and B . Cell-surface CAR levels following ZEB1 knockdown were measured by flow cytometry on PANC-1 cells treated with TGF-β1 ( A ), or on MDA-MB-231 cells ( B ). Error bars represent standard deviations (biological duplicates). C and D . Ad-GFP uptake following ZEB1 knockdown was determined by flow cytometry measuring GFP levels ( C ), or by real-time PCR for virus copy number ( D ). Error bars represent standard deviations. A-D . CAR and GFP signals are expressed as geometric means of the fluorescence signal intensities normalized to the untreated controls (A-C), virus copy numbers as relative adenovirus fiber levels (D). Abbreviations: UT, untransfected; Ctrl #1, siControl ON-TARGETplus Non-targeting siRNA #1 (Dharmacon); Ctrl #2, firefly luciferase-targeting siRNA; ZEB1 siRNA #1/#2, ZEB1-targeting siRNAs. Ctrl #2 and ZEB1 siRNA sequences are provided in Additional file 1 (Table S3). p

    Article Snippet: Flow cytometry Live cells were stained with an anti-CAR-phycoerythrin (PE) antibody (E1-1, mouse monoclonal; Santa Cruz Biotechnology, Inc.) or PE-conjugated control IgG-PE (mouse monoclonal IgG1 κ, Pharmingen/BD Biosciences) while rotating for 60 minutes at 4°C.

    Techniques: Flow Cytometry, Cytometry, Multiple Displacement Amplification, Real-time Polymerase Chain Reaction, Fluorescence, Luciferase