rabbit polyclonal anti ca v 1 2 (Alomone Labs)


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Rabbit Polyclonal Anti Ca V 1 2, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit polyclonal anti ca v 1 2/product/Alomone Labs
Average 96 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
1) Product Images from "A maladaptive feedback mechanism between the extracellular matrix and cytoskeleton contributes to hypertrophic cardiomyopathy pathophysiology"
Article Title: A maladaptive feedback mechanism between the extracellular matrix and cytoskeleton contributes to hypertrophic cardiomyopathy pathophysiology
Journal: Communications Biology
doi: 10.1038/s42003-022-04278-9

Figure Legend Snippet: Immunoblot analysis of L-type calcium channel ( a , b ) and β 1 integrin ( c , d ) protein expression performed on total heart homogenate pooled from groups of 5 pre- (10–15-wk-old) or post-cardiomyopathic (30–50-week-old) cTnI-G203S mice and age-matched wt counterparts. Representative immunoblots probed with L-type calcium channel α 1C subunit (Ca V 1.2, a ) or β 1 integrin ( c ) antibody, then GAPDH monoclonal antibody. Densitometry analysis of Ca V 1.2 ( b ) or β 1 integrin ( d ) protein expression, normalized to associated GAPDH expression. n = number of technical repeats. A Browne-Forsythe and Welch ANOVA ( b ) or Kruskal-Wallis test ( d ) determined statistical significance. Densitometry analysis of relative mTOR expression (calculated as Phospho-mTOR/Total mTOR) performed on cytoplasmic ( e ) and nuclear ( f ) fractions pooled from groups of five pre- or post-cardiomyopathic cTnI-G203S mice and age-matched wt counterparts. β-tubulin and histone H2B antibodies were used as loading controls for cytoplasmic and nuclear fractions respectively. n = number of technical repeats. A Browne-Forsythe and Welch ANOVA ( e ) or Kruskal-Wallis test ( f ) determined statistical significance. g Schematic indicating structural-functional link between the L-type calcium channel, cytoskeletal network, mitochondria, integrin and the extracellular matrix in wt and cTnI-G203S cardiac myocytes. A disrupted cytoskeletal architecture in cTnI-G203S cardiac myocytes may trigger a maladaptive feedback mechanism between increased cytoskeletal and extracellular matrix stiffness, resulting in a hypermetabolic mitochondrial state.
Techniques Used: Western Blot, Expressing, Functional Assay
rabbit anti ca v 1 2 (Alomone Labs)


Structured Review

Rabbit Anti Ca V 1 2, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit anti ca v 1 2/product/Alomone Labs
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
1) Product Images from "Muscarinic Receptor Stimulation Does Not Inhibit Voltage-dependent Ca 2+ Channels in Rat Adrenal Medullary Chromaffin Cells"
Article Title: Muscarinic Receptor Stimulation Does Not Inhibit Voltage-dependent Ca 2+ Channels in Rat Adrenal Medullary Chromaffin Cells
Journal: Acta Histochemica et Cytochemica
doi: 10.1267/ahc.23-00042

Figure Legend Snippet: Different distribution of Ca V 1.2 channel and muscarinic M 4 receptor in the cell membrane in rat AMC cells. ( A ) Immunostaining of Ca V 1.2-YFP fusion protein expressed in HEK293T cells with a rabbit anti-Ca V 1.2 antibody (Ab). HEK293T transfected with a Ca V 1.2-YFP construct were labeled with the rabbit anti-Ca V 1.2 Ab. a and b represent confocal images of Ca V 1.2-like immunofluorescence and YFP fluorescence, respectively; c represents a differential interference contrast (DIC) image. The immunoreaction and YFP fluorescence were visualized with excitation at 514 nm and emission of 530–600 nm and with excitation at 633 and emission above 650 nm, respectively. ( B ) Fractionation analysis of rat adrenal medullae for integral membrane proteins. The cell membrane was divided into the raft and non-raft membrane domains by using discontinuous sucrose density gradient centrifugation (see the Materials and Methods). The same volume of each fraction with 5%–40% sucrose was immunoblotted for caveolin-1, transferrin receptor (R), muscarinic M 4 receptor, and TASK1 channel. Note that caveolin-1, a raft membrane marker, was enriched in the 20% fraction, whereas transferrin R, a non-raft membrane marker, was present in the 40% fraction. ( C ) Double staining for caveolin-1 and Ca V 1.2 and for M 4 receptor and Ca V 1.2 in rat AMC cells. The first column indicates confocal images of caveolin-1 and M 4 receptor-like immunofluorescence. The second column shows confocal images of Ca V 1.2-like immunofluorescence. The third column is a merge of immunofluorescence images. The fourth column shows DIC images. The calibration applies to all the images. Dissociated rat AMC cells were treated overnight with rabbit anti-Ca V 1.2 Ab (dilution, 1:50) and mouse anti-caveolin-1 Ab (1:20) or mouse anti-M 4 Ab (1:50). Ca V 1.2 and caveolin-1 or M 4 receptor-like immunoreactive material were visible as rhodamine and FITC-like fluorescence, respectively. ( D ) Summary of the coincidence rates of caveolin-1 (Cav1) and M 4 with Ca V 1.2. The data represent the mean ± SEM (Cav1/Ca V 1.2, n = 10; M 4 /Ca V 1.2, n = 5). Statistical significance was evaluated with an unpaired Student’s t test.
Techniques Used: Membrane, Immunostaining, Transfection, Construct, Labeling, Immunofluorescence, Fluorescence, Fractionation, Gradient Centrifugation, Marker, Double Staining

Figure Legend Snippet: Diagram showing localization of caveolin-1, Ca V 1.2, muscarinic M 4 receptor subtype, and TASK1 in the raft and non-raft membrane domains.
Techniques Used: Membrane
anti ca v 1 2 (Alomone Labs)


Structured Review

Anti Ca V 1 2, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti ca v 1 2/product/Alomone Labs
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
1) Product Images from "A novel binding site between the voltage-dependent calcium channel Ca V 1.2 subunit and Ca V β2 subunit discovered using a new analysis method for protein–protein interactions"
Article Title: A novel binding site between the voltage-dependent calcium channel Ca V 1.2 subunit and Ca V β2 subunit discovered using a new analysis method for protein–protein interactions
Journal: Scientific Reports
doi: 10.1038/s41598-023-41168-4

Figure Legend Snippet: Figure 2 (a) Strategy for creating a Ca V 1.2 epitope library with the β2 subunit. Full-length cDNA of Ca V β2 gene is in the arabinose unit and the Ca V 1.2 epitope library is in the IPTG unit. ( b ) Construction of a Ca V 1.2 epitope-library (IPTG-sensitive toxin-in-frame clone library). ( i ) Blunt-ended DNA fragment from Ca V 1.2 is inserted at the Sma I site (with a single frameshift with the first iUnit) of the IPTG unit. ( ii ) Clones with the first iUnit (first library). ( iii ) First iUnit in the library is deleted using Spe I and re-ligated. Only clones with the chimeric gene and neomycin-resistance gene form colonies (second library). ( iv ) Neomycin-resistance gene is eliminated using Xho I and re-ligated (third library).
Techniques Used: Clone Assay

Figure Legend Snippet: Results of third screening of pdGENE-Toxin sensitivity assay. ( a ) Candidate clones with selection. Selection by ampicillin, arabinose, and IPTG, or ampicillin and arabinose. #23, #24, #30, #31, #32, #36, #37 and #46 form colonies under ampicillin/arabinose/IPTG and ampicillin/arabinose. Negative control was clone #2, which was IPTG-sensitive (negative selection). Sequence direction and corresponding sequences are indicated. Clones in the antisense direction (#23, 32, and 36) and those with an E. coli -derived sequence were eliminated. Clone #37 was eliminated because it contained a Ca V 1.2 transmembrane region. ( b ) Overlapping sequence of clones 24 and 31. Translated amino acid sequences are above nucleotide sequences (single-letter code) and numbered.
Techniques Used: Sensitive Assay, Clone Assay, Selection, Negative Control, Sequencing, Derivative Assay

Figure Legend Snippet: Coimmunoprecipitation analysis of interactions between the β2 subunit and Ca V 1.2-derived clone (24) in human embryonic kidney 293 (HEK-293) cells. ( a ) Western blot analyses of Ca V β2 in HEK-293 T cells. Transected constructs are indicated. Lane, 1 Ca V β2; lane 2, Ca V β2 and EGFP-24; lane 3, Ca V β2 and full-length Ca V 1.2. ( b ) Coimmunoprecipitation analysis of EGFP-24 and Ca V β2. An anti-GFP antibody revealed a single band that corresponded to the EGFP-24 fusion protein (lane 2). ( c ) Coimmunoprecipitation analysis of Ca V 1.2 and Ca V β2. An anti-Ca V 1.2 antibody revealed a single band (lane 3, 200 kDa).
Techniques Used: Derivative Assay, Western Blot, Construct

Figure Legend Snippet: ( a ) Amino acid sequence of the C-terminus of Ca V 1.2. The IQ domain (light green), CAC1F_C domains (red), C-terminal binding site (CBS), distal C-terminal regulatory domain (black underlined), leucine-zipper-like region (red asterisks), and CaM-competitive domain (green underlined) were obtained from the NCBI database ( https://www.ncbi.nlm.nih.gov/Structure/cdd/wrpsb.cgi ). ( b ) Schematic of Ca V 1.2. The four transmembrane domains are labelled I to IV. The high-affinity interaction domain (α-interaction domain; AID) is located between domains I and II. The IQ domain, CAC1F domain, and C-terminal binding site (CBS) are located at the C-terminus.
Techniques Used: Sequencing, Binding Assay

Figure Legend Snippet: ( a ) Protein alignment of the C-terminal binding sites (CBS) of rabbit α1 subunits. Amino acid sequence alignments of Ca V 1.1, Ca V 1.2, Ca V 1.3, Ca V 1.4, Ca V 2.1, Ca V 2.2, Ca V 2.3, and Ca V 3.1 obtained using ClustalW ( https://www.genome.jp/tools-bin/clustalw ). The CBS in Ca V 1.2 is in red. Protein IDs are indicated. *, identity; -, gap. Alignment of the CBS of rabbit Ca V 1.2 and Ca V 1.3 (inset). CBS sequence of Ca V 1.3 shows homology with that of Ca V 1.2. ( b ) Amino acid alignment of the CBS of Ca V 1.2 in different species. CBS sequences of zebrafish, xenopus, human, rabbit, mouse, and rat were aligned using ClustalW ( https://www.genome.jp/tools-bin/clustalw ). CBS in rabbit Ca V 1.2 is in red. Protein IDs are indicated. *, identity; -, gap.
Techniques Used: Binding Assay, Sequencing
ca v 1 2 (Alomone Labs)


Structured Review

Ca V 1 2, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ca v 1 2/product/Alomone Labs
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
1) Product Images from "NPC1-dependent alterations in K V 2.1–Ca V 1.2 nanodomains drive neuronal death in models of Niemann-Pick Type C disease"
Article Title: NPC1-dependent alterations in K V 2.1–Ca V 1.2 nanodomains drive neuronal death in models of Niemann-Pick Type C disease
Journal: Nature Communications
doi: 10.1038/s41467-023-39937-w

Figure Legend Snippet: A Left: Representative super-resolution Airyscan images taken at a focal plane near the plasma membrane (PM) from CTL (black) and U18-treated (red) neurons fixed and immunolabeled for Ca V 1.2. Right , quantification of PM Ca V 1.2 total intensity, cluster density and cluster size of CTL (black) and U18-treated (red) neurons in the soma (left, yellow) and dendrite (right, orange) regions. N = 46–48 (CTL) and n = 58–59 (U18) neurons, and n = 145 (CTL) and n = 161 (U18) dendrites were analyzed across 5 independent isolations. B Left , representative super-resolution TIRF localization maps of CTL (black) and U18-treated (red) neurons fixed and immunolabeled for Ca V 1.2. Right , quantification of PM Ca V 1.2 cluster density, cluster size, and nearest cluster distance of CTL (black) and U18-treated (red) neurons in the soma region. N = 20 (CTL) and n = 21 (U18) neurons were analyzed across 3 independent isolations. C Same as ( A ) only neurons fixed and immunolabeled for Ca V 1.3. N = 32 (CTL) and n = 38 (U18) neurons, and n = 104 (CTL) and n = 123 (U18) dendrites were analyzed across 3 independent isolations. D Same as B, only immunolabeled for Ca V 1.3. N = 16 (CTL) and n = 18 (U18) neurons were analyzed across 2 independent isolations. All error bars represent SEM. Statistical significance was calculated using Mann–Whitney (two-tailed) and Unpaired t tests (two-tailed) in ( A )–( D ). ns: not significant; * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001. CTL is control and U18 is U18666A.
Techniques Used: Immunolabeling, MANN-WHITNEY, Two Tailed Test

Figure Legend Snippet: A Top , representative super-resolution TIRF images of CTL (black) and U18-treated (red) neurons co-immunolabeled for Ca V 1.2 and K V 2.1. Bottom , quantification of % of soma occupied by K V 2.1–Ca V 1.2, K V 2.1–Ca V 1.2 cluster density and K V 2.1–Ca V 1.2 nearest cluster distance of CTL (black) and U18-treated (red) neurons in the soma region. N = 9 (CTL) and n = 10 (U18) neurons were analyzed across 2 independent isolations. B Top , representative Ca V 1.2 and K V 2.1 PLA images of CTL (black) and U18-treated (red) neurons. Bottom , quantification of PLA puncta density and nearest puncta distance of CTL (black) and U18-treated (red) neurons. N = 19 (CTL) and n = 17 (U18) neurons were analyzed across 2 independent isolations. C Left , representative maximum intensity projections from WT (black) and NPC1 I1061T (red) cerebellar sagittal sections co-immunolabeled for Calbindin, Ca V 1.2 and K V 2.1. Right , quantification of K V 2.1–Ca V 1.2 colocalization volume in WT (black) and NPC1 I1061T (red) neurons in the soma region. n = 47–48 (WT and NPC1 I1061T ) neurons were analyzed across 3 animals. D Diagram detailing HA-TAT-Ca 2+ Channel Association Domain (CCAD) mode of action. E Left , representative super-resolution Airyscan images taken at a focal plane near the PM of CTL (black) and U18 (red) neurons incubated with the CCAD or HA-TAT-Scr scrambled peptide (SCRBL), and co-immunolabeled for Ca V 1.2 and HA. Right , quantification of PM Ca V 1.2 cluster size of CTL (black) and U18-treated (red) neurons co-incubated with CCAD or SCRBL peptide in the soma (left, yellow) and dendrite (right, orange) regions. N = 18 (SCRBL), n = 30 (SCRBL + U18), n = 25 (CCAD) and n = 19 (CCAD + U18) neurons and n = 46 (SCRBL), n = 55 (SCRBL + U18), n = 57 (CCAD) and n = 34 (CCAD + U18) dendrites were analyzed across 2 independent isolations. All error bars represent SEM. Statistical significance was calculated using Mann–Whitney (two-tail) and Unpaired t tests (two-tail) in ( A )–( C ) and two-way ANOVA in ( E ); ns: not significant; * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001. CTL is control, SCRBL is scramble, U18 is U18666A, and CCAD is calcium channel association domain.
Techniques Used: Immunolabeling, Incubation, MANN-WHITNEY

Figure Legend Snippet: A Schematic diagram detailing roscovitine (Rosc) mode of action. B Top , representative super-resolution Airyscan images taken at a focal plane near the PM of CTL (black) or U18-treated (red) neurons co-incubated with roscovitine (Rosc) (cyan) and co-immunolabeled for Ca V 1.2 and K V 2.1. Bottom , quantification of PM KV2.1 and Ca V 1.2 clustering size, and % of the soma occupied by Ca V 1.2–K V 2.1 (left, yellow) and Ca V 1.2–K V 2.1 area in the dendrite region (right, orange) of CTL (black), U18 (red) and Rosc (cyan) neurons. N = 43 (CTL), n = 41 (U18), n = 43 (Rosc) and n = 38 (Rosc+U18) neurons and n = 89 (CTL), n = 94 (U18), n = 82 (Rosc) and n = 71 (Rosc+U18) dendrites were analyzed across 5 independent isolations. All error bars represent SEM. Statistical significance was calculated using a two-way ANOVA test. ns: not significant; * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001. # P < 0.05; ## P < 0.01; #### P < 0.0001. # indicates comparison with the CTL condition. CTL is control, U18 is U18666A, ROSC is Roscovitine.
Techniques Used: Incubation, Immunolabeling

Figure Legend Snippet: A Schematic diagram of the hypothesis: NPC1 deficient neurons have ER-PM domains enriched in RyR—Ca V 1.2–K v 2.1—SERCA and increased spontaneous Ca 2+ activity at K V 2.1-asociated ER – PM MCSs. B Left , representative super-resolution Airyscan images taken at a focal plane near the PM of CTL (black) and U18-teated (red) neurons co-immunolabeled for Ca V 1.2 and RyR. Right , quantification of % soma occupied by Ca V 1.2–RyR, Ca V 1.2–RyR cluster density and Ca V 1.2–RyR cluster size of CTL (black) and U18-treated (red) neurons in the soma (top, yellow) and dendrite (bottom, orange) region. N = 20–21 (CTL) and n = 26 (U18) neurons and n = 52–53 (CTL) and n = 54–55 (U18) dendrites were analyzed across 2 indepdendent isolations. C Top , schematic diagram of hypothesis: NPC1 deficient neurons (U18, red) have increased Ca 2+ activity and K V 2.1-associated Ca V 1.2, while disrupting K v 2.1–Ca v 1.2 or K V 2.1–VAPA/B interactions (CCAD, purple or FFAT, red-brown) abrogates such effects. Middle left , representative super-resolution TIRF images of neurons transfected with GCamP3-Kv2.1 P4O4W . Bottom left , intensity time series and kymographs of spontaneous activity taken from the square region of interest. Middle right , quantification of GCamP3-Kv2.1 P4O4W peak amplitude of CTL (black), U18 (red), CCAD + U18 (purple) and FFAT + U18 (red-brown) neurons. N = 85 (CTL), n = 141 (U18), n = 23 (CCAD + U18) and n = 10 (FFAT + U18) GCamP3-Kv2.1 P4O4W peaks were analyzed across 3 independent isolations. All error bars represent SEM. Statistical significance was calculated using the following tests: Unpaired (two-tail) and Mann–Whitney t tests (two-tail) in ( B ) and Kruskal–Wallis test in ( C ). ns: not significant; * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001. # P < 0.05; ## P < 0.01; #### P < 0.0001. # indicates comparison with the CTL condition. CTL is control, SCRBL is scramble, U18 is U18666A, CCAD is calcium channel association domain, and FFAT is two phenylalanines (FF) in an acidic tract.
Techniques Used: Activity Assay, Immunolabeling, Transfection, MANN-WHITNEY
ca v 1 2 α 1c (Alomone Labs)


Structured Review

Ca V 1 2 α 1c, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ca v 1 2 α 1c/product/Alomone Labs
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
1) Product Images from "Dysregulated Rbfox2 produces aberrant splicing of Ca V 1.2 calcium channel in diabetes-induced cardiac hypertrophy"
Article Title: Dysregulated Rbfox2 produces aberrant splicing of Ca V 1.2 calcium channel in diabetes-induced cardiac hypertrophy
Journal: Cardiovascular Diabetology
doi: 10.1186/s12933-023-01894-5

Figure Legend Snippet: Ca V 1.2 alternative exon 9* is specifically increased in diabetic heart . ( A ) The membrane expression of Ca V 1.2 α 1C was detected by Western blotting in heart tissues from control and HFD/STZ-treated rats, Na-K ATPase protein was detected as internal control. The relative band densities were analyzed and normalized to Na-K ATPase. n = 6 rats for each group. * P = 0.0166, unpaired t test. ( B ) Schematic diagram shows the locations of the PCR primers designed to amplify and detect rat Ca V 1.2 inclusive of or in the absence of alternative exons in cardiac tissues. Total RNA was extracted from hearts, and PCR products amplified from cDNA libraries were separated on 2.5% agarose gel. Actb mRNA was detected as internal control. Rat Cacna1c mRNAs with exon 8 or 8a were amplified by RT-PCR, followed by digestion with restriction endonuclease BamHI. The value for percent exon 8a inclusion were the lower 2 bands’ intensity divided by the sum of the intensities of upper and lower bands. n = 4 rats for each group. P = 0.4107, unpaired t test. ( C ) The value for percent exon 9* inclusion was the upper band intensity divided by the summed intensities of upper and lower bands. n = 6 rats for each group. **P < 0.0001, unpaired t test. ( D ) The value for percent exon 33 inclusion was also presented as a bar chart. n = 6 rats for each group. P = 0.4107, unpaired t test. ns indicates no significant differences
Techniques Used: Expressing, Western Blot, Amplification, Agarose Gel Electrophoresis, Reverse Transcription Polymerase Chain Reaction

Figure Legend Snippet: Rbfox2 specifically modulates Ca V 1.2 alternative exon 9* splicing in cardiomyocyte . ( A ) H9c2 cells were transfected with nontargeting (NT) or Rbfox2 siRNA for 48 h. The endogenous expression of Rbfox2 protein was detected by Western blotting, the β-actin was detected as internal control. PCR product of Ca V 1.2 E9* channel was amplified from cDNA libraries and separated on 2.5% agarose gel. Actb mRNA was detected as internal control. ( B ) The relative expression of Rbfox2 was normalized to β-actin. ( C ) The value for percent Ca V 1.2 E9* inclusion was the upper band intensity divided by the summed intensities of upper and lower bands, and presented as a bar chart. ( D ) H9c2 cells were transfected with an empty vector, WT Rbfox2, DN Rbfox2 or WT plus DN Rbfox2 expression plasmids, nontreated cells were set as negative control (NC). After 48 h incubation, the expression of Rbfox2 protein was detected by Western blotting, the β-actin was detected as internal control. PCR products were separated on 2–3% agarose gel, which was used to check the proportions of Ca V 1.2 E9* channels. ( E ) Relative expression of Rbfox2 was normalized to β-actin. ( F ) The proportion of Ca V 1.2 E9* channels were analyzed and presented by a bar chart. n = 3 independent experiments. * P < 0.05, **P < 0.01, one-way ANOVA followed by a Tukey’s post hoc test
Techniques Used: Transfection, Expressing, Western Blot, Amplification, Agarose Gel Electrophoresis, Plasmid Preparation, Negative Control, Incubation

Figure Legend Snippet: Glycated serum (GS), not D-glucose, decreases Rbfox2 expression but increases Ca V 1.2 E9* channels . ( A ) NRVMs were treated with mannitol or D-glucose, nontreated cells were set as control. After 48 h incubation, the endogenous expression of Rbfox2 protein was detected by Western blotting, the β-actin was detected as internal control. ( B ) Relative Rbfox2 expression was normalized with β-actin in differentially-treated cells. n = 4 independent experiments. P = 0.9854, one-way ANOVA followed by a Tukey’s post hoc test. ( C ) PCR products amplified from cDNA libraries of differentially-treated NRVM were separated on 2.5% agarose gel, and the values of proportion of Ca V 1.2 E9* were analyzed. n = 4 independent experiments. P = 0.9109, one-way ANOVA followed by a Tukey’s post hoc test. ( D ) Serum levels of advanced glycation end-products (AGEs) were measured by an ELISA kit in control (n = 5) and HFD/STZ-treated rats (n = 6). ** P = 0.0042, unpaired t test. ( E ) The levels of AGEs in the lysate of cardiac tissues from different rats’ models (n = 6 each group) were also measured by ELISA. ** P = 0.0027, unpaired t test. ( F ) NRVMs were treated with 10% non-glycated (NG) or glycated serum (GS) for 48 h, after that the cells were harvested for detecting Ca V 1.2 alternative exon 9* and expression of Rbfox2 by RT-PCR and Western blotting, respectively. ( G ) Relative expression of Rbfox2 was also analyzed. n = 3 independent experiments. * P = 0.0478, unpaired t test. ( H ) The values for percent Ca V 1.2 E9* channels were presented. n = 6 independent experiments. P < 0.0001, unpaired t test. ( I - J ) WT and DN Rbfox2 mRNA expression were also determined by real-time RT-PCR in NRVMs treated with NG or GS. Actb mRNA was measured as internal control. n = 5–6 independent experiments. **P < 0.01, unpaired t test. ns indicates no significant differences
Techniques Used: Expressing, Incubation, Western Blot, Amplification, Agarose Gel Electrophoresis, Enzyme-linked Immunosorbent Assay, Reverse Transcription Polymerase Chain Reaction, Quantitative RT-PCR

Figure Legend Snippet: GS application induces hyperpolarized window currents of Ca V 1.2 channel in NRVMs . ( A ) NRVMs were treated with 10% NG or GS for 48 h, then the membrane protein was extracted. Ca V 1.2 α 1C subunit was detected, and Na-K ATPase was detected as internal control. ( B ) Relative expression of Ca V 1.2 α 1C subunit was normalized with Na-K ATPase. n = 3 independent experiments. P = 0.7251, unpaired t test. ns indicates no significant differences. ( C ) Ca V 1.2 channel currents were recorded from NRVMs with NG, GS or GS + Gö6983 treatment in 10 mmol/L Ba 2+ bath solution. ( D ) I-V relationship curves of Ca V 1.2 channel were recorded under different testing potentials in NRVMs. ( E ) Current densities of Ca V 1.2 channel in NRVMs treated with NG, GS or GS + Gö6983 were presented. ( F ) Plots of steady-state activation (SSA) curve of Ca V 1.2 channel were derived from I - V currents in differentially-treated NRVMs. ( G ) Plots of the steady-state inactivation (SSI) curve were recorded and analyzed in NRVMs. ( H ) Window currents were superimposed from SSI ( f ∞ ) and SSA ( d ∞ ) curves of NG, GS or GS + Gö6983-treated NRVMs
Techniques Used: Expressing, Activation Assay, Derivative Assay

Figure Legend Snippet: Knockdown of Rbfox2 hyperpolarizes window currents of Ca V 1.2 channel in NRVMs . ( A ) The protein expression of Rbfox2 and β-actin were detected in whole-cell lysate of isolated NRVMs by using Western blotting after transfecting with NT or Rbfox2 siRNAs. The membrane protein was also extracted, and membrane expression of Ca V 1.2 α 1C was checked, Na-K ATPase was detected as a membrane loading control. ( B ) Relative expression level of Rbfox2 was normalized with β-actin in differentially transfected cells, and presented as a bar chart. n = 4 independent experiments. ** P < 0.001, one-way ANOVA followed by a Tukey’s post hoc test. ( C ) Relative Ca V 1.2 α 1C membrane expression was normalized with Na-K ATPase in differentially-transfected cells. n = 4 independent experiments. P = 0.6679, one-way ANOVA followed by a Tukey’s post hoc test. ( D ) Raw traces of Ca V 1.2 whole-cell calcium current recorded from NRVMs treated with NT or Rbfox2 siRNA in 10 mmol/L Ba 2+ external solution. ( E ) I-V relationship of calcium channel current recorded under the different testing potential, increased from − 50 to 50 mV in NRVMs transfected with NT or Rbfox2 siRNA. ( F ) Ca V 1.2 channel current density in NRVMs was analyzed after transfected with NT or Rbfox2 siRNA. ( H ) Plots of steady-state activation (SSA) curve of Ca V 1.2 channel were analyzed from I-V currents in NT or Rbfox2 siRNA-treated NRVMs. ( H ) Plots of the steady-state inactivation (SSI) was also recorded in NRVMs. ( I ) Ca V 1.2 window currents were superimposed from SSI ( f ∞ ) and SSA ( d ∞ ) curves of NRVMs
Techniques Used: Expressing, Isolation, Western Blot, Transfection, Activation Assay
![Knockdown of Rbfox2 induces cardiomyocyte hypertrophy . ( A ) NRVMs treated with Knockdown of Rbfox2 induces cardiomyocyte hypertrophy . ( A ) NRVMs treated with NT or Rbfox2 siRNAs, real-time [Ca 2+ ] i was measured by Ca 2+ fluorescence indicator Fluo-4 AM, Δ[Ca 2+ ] i fluorescence intensities were measured by dividing the changes in the fluorescent signal by the average resting fluorescence. ( B ) Plots of time course of fluorescent intensity after application with NT (n = 20 cells) or Rbfox2 siRNAs (n = 13 cells) in NRVMs from 3 independent experiments, Δ[Ca 2+ ] i was presented as Δ F / F 0 . * P < 0.05, two-way ANOVA followed by Sidak’s multiple comparisons test. ( C ) Representative images of NRVMs transfected with NT or Rbfox2 siRNA, immunofluorescence staining by using anti-a-actinin antibody was applied to determine cell surface area (CSA). ( D ) Analyzed CSA was shown as a bar chart in NT (n = 18 cells) or Rbfox2 siRNA (n = 18 cells) transfected NRVMs from 3 independent experiments. ** P = 0.0005, unpaired t test. ( E ) Rat Nppa, Nppb and Myh7 mRNAs were determined by real-time RT-PCR in differentially-treated NRVMs, rat Actb mRNA was detected as internal control. n = 3–4 independent experiments. * P < 0.05, ** P < 0.01, unpaired t test. ( F ) Illustration of main findings in this study. Under diabetic hyperglycemia, AGEs, not glucose, induces aberrant expression of Rbfox2, which upregulates Ca V 1.2 E9* channels in cardiomyocyte. AGEs/Rbfox2-mediated AS hyperpolarizes cardiac Ca V 1.2 window currents, elevates K + -triggered [Ca 2+ ] i and promotes fetal genes’ transcription, finally induces cardiomyocyte hypertrophy. RAGE, receptor for advanced glycation end-products; RyR2, ryanodine receptor 2; SR, sarcoplasmic reticulum](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_4275/pmc10324275/pmc10324275__12933_2023_1894_Fig9_HTML.jpg)
Figure Legend Snippet: Knockdown of Rbfox2 induces cardiomyocyte hypertrophy . ( A ) NRVMs treated with NT or Rbfox2 siRNAs, real-time [Ca 2+ ] i was measured by Ca 2+ fluorescence indicator Fluo-4 AM, Δ[Ca 2+ ] i fluorescence intensities were measured by dividing the changes in the fluorescent signal by the average resting fluorescence. ( B ) Plots of time course of fluorescent intensity after application with NT (n = 20 cells) or Rbfox2 siRNAs (n = 13 cells) in NRVMs from 3 independent experiments, Δ[Ca 2+ ] i was presented as Δ F / F 0 . * P < 0.05, two-way ANOVA followed by Sidak’s multiple comparisons test. ( C ) Representative images of NRVMs transfected with NT or Rbfox2 siRNA, immunofluorescence staining by using anti-a-actinin antibody was applied to determine cell surface area (CSA). ( D ) Analyzed CSA was shown as a bar chart in NT (n = 18 cells) or Rbfox2 siRNA (n = 18 cells) transfected NRVMs from 3 independent experiments. ** P = 0.0005, unpaired t test. ( E ) Rat Nppa, Nppb and Myh7 mRNAs were determined by real-time RT-PCR in differentially-treated NRVMs, rat Actb mRNA was detected as internal control. n = 3–4 independent experiments. * P < 0.05, ** P < 0.01, unpaired t test. ( F ) Illustration of main findings in this study. Under diabetic hyperglycemia, AGEs, not glucose, induces aberrant expression of Rbfox2, which upregulates Ca V 1.2 E9* channels in cardiomyocyte. AGEs/Rbfox2-mediated AS hyperpolarizes cardiac Ca V 1.2 window currents, elevates K + -triggered [Ca 2+ ] i and promotes fetal genes’ transcription, finally induces cardiomyocyte hypertrophy. RAGE, receptor for advanced glycation end-products; RyR2, ryanodine receptor 2; SR, sarcoplasmic reticulum
Techniques Used: Fluorescence, Transfection, Immunofluorescence, Staining, Quantitative RT-PCR, Expressing
anti ca v 1 2 α1c subunit antibodies (Alomone Labs)


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Anti Ca V 1 2 α1c Subunit Antibodies, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti ca v 1 2 α1c subunit antibodies/product/Alomone Labs
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
1) Product Images from "Palmitoylation of the pore-forming subunit of Ca(v)1.2 controls channel voltage sensitivity and calcium transients in cardiac myocytes"
Article Title: Palmitoylation of the pore-forming subunit of Ca(v)1.2 controls channel voltage sensitivity and calcium transients in cardiac myocytes
Journal: Proceedings of the National Academy of Sciences of the United States of America
doi: 10.1073/pnas.2207887120

Figure Legend Snippet: Ca(v) 1.2 α1C subunit is palmitoylated in mouse, rabbit, and human ventricular tissues. Palmitoylated proteins were purified by resin-assisted capture of acylated proteins (acyl-RAC) and immunoblotted as shown. The bar chart below each blot indicates the abundance of Ca(v)1.2 α1C and caveolin 3 (Cav3) in the purified palmitoylated fraction (Palm) relative to the corresponding unfractionated lysate (UF). N = 4 (mouse), N = 8 (rabbit), N = 7 (human). *** P < 0.001, unpaired t test. Error bars represent SEM.
Techniques Used: Purification

Figure Legend Snippet: Palmitoylation site conservation in human Ca(v)1 channel isoforms. For clarity, regions of the rabbit α1C splice variant of Ca(v)1.2 CACH2A (UniProt accession number P15381) with palmitoylated cysteines identified in this investigation numbered and highlighted in red are shown above the corresponding regions of the human channels. Numbers at the end of each sequence are the numbering of the final amino acid in the region of each Ca(v)1 isoform shown. Human Ca(v)1 isoforms (UniProt accession numbers shown) were aligned using Clustal Ω. “*” below an amino acid indicates 100% conservation between isoforms; “:” indicates amino acids of highly similar properties; “.” indicates amino acids of weakly similar properties. The palmitoylation site in the Ca(v)1.2 N terminus is conserved in all isoforms. Ca(v)1.1 does not possess a cysteine analogous to C519 in the I–II linker, but Ca(v)1.3 and 1.4 do. C543 is unique to Ca(v)1.2.
Techniques Used: Variant Assay, Sequencing
rabbit polyclonal anti ca v 1 2 (Alomone Labs)


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Rabbit Polyclonal Anti Ca V 1 2, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit polyclonal anti ca v 1 2/product/Alomone Labs
Average 96 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
1) Product Images from "A maladaptive feedback mechanism between the extracellular matrix and cytoskeleton contributes to hypertrophic cardiomyopathy pathophysiology"
Article Title: A maladaptive feedback mechanism between the extracellular matrix and cytoskeleton contributes to hypertrophic cardiomyopathy pathophysiology
Journal: Communications Biology
doi: 10.1038/s42003-022-04278-9

Figure Legend Snippet: Immunoblot analysis of L-type calcium channel ( a , b ) and β 1 integrin ( c , d ) protein expression performed on total heart homogenate pooled from groups of 5 pre- (10–15-wk-old) or post-cardiomyopathic (30–50-week-old) cTnI-G203S mice and age-matched wt counterparts. Representative immunoblots probed with L-type calcium channel α 1C subunit (Ca V 1.2, a ) or β 1 integrin ( c ) antibody, then GAPDH monoclonal antibody. Densitometry analysis of Ca V 1.2 ( b ) or β 1 integrin ( d ) protein expression, normalized to associated GAPDH expression. n = number of technical repeats. A Browne-Forsythe and Welch ANOVA ( b ) or Kruskal-Wallis test ( d ) determined statistical significance. Densitometry analysis of relative mTOR expression (calculated as Phospho-mTOR/Total mTOR) performed on cytoplasmic ( e ) and nuclear ( f ) fractions pooled from groups of five pre- or post-cardiomyopathic cTnI-G203S mice and age-matched wt counterparts. β-tubulin and histone H2B antibodies were used as loading controls for cytoplasmic and nuclear fractions respectively. n = number of technical repeats. A Browne-Forsythe and Welch ANOVA ( e ) or Kruskal-Wallis test ( f ) determined statistical significance. g Schematic indicating structural-functional link between the L-type calcium channel, cytoskeletal network, mitochondria, integrin and the extracellular matrix in wt and cTnI-G203S cardiac myocytes. A disrupted cytoskeletal architecture in cTnI-G203S cardiac myocytes may trigger a maladaptive feedback mechanism between increased cytoskeletal and extracellular matrix stiffness, resulting in a hypermetabolic mitochondrial state.
Techniques Used: Western Blot, Expressing, Functional Assay
rabbit anti ca v 1 2 (Alomone Labs)


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Rabbit Anti Ca V 1 2, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit anti ca v 1 2/product/Alomone Labs
Average 93 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
1) Product Images from "Modulation of Calcium-Dependent Inactivation of L-Type Ca 2+ Channels via β-Adrenergic Signaling in Thalamocortical Relay Neurons"
Article Title: Modulation of Calcium-Dependent Inactivation of L-Type Ca 2+ Channels via β-Adrenergic Signaling in Thalamocortical Relay Neurons
Journal: PLoS ONE
doi: 10.1371/journal.pone.0027474

Figure Legend Snippet: ( A ) Representative current traces recorded under control conditions (upper left panel) and in the presence of the specific β 2 AR agonist salmeterol alone (10 µM; left middle panel) or in combination with PKI 14–22 amide (10 µM; lower left panel). The bar graph represents D inact under different recording conditions (as indicated). The mean value of five cells recorded under control conditions was taken for comparison with four cells recorded under 10 µM salmeterol and five cells recorded under 10 µM salmeterol plus PKI 14–22 amide. Data are presented as means ± SEM of several independent experiments. *** P <0.001. Significance of salmeterol plus PKI (n = 5) versus salmeterol alone (n = 4) was calculated by Student's t test. The degree of inactivation is given by the normalized current amplitude of the mean postpulse I/V at +10 mV. ( B ) Close co-expression of the main modulator of CDI, PKA (green) and Ca V 1.2 (red) in cultured neurons. Yellow dots represent places were these two proteins are in close proximity. Data shown are representative pictures from several independent immunostainings and preparations of neurons. In all cases, omission of primary antibodies resulted without signal (negative control). ( C ) Indicated brain regions were immunostained with antibodies specific for PKARIIβ and Ca V 1.2. Thalamic regions LGN and VB revealed very strong interaction patterns in merged pictures. Association of these proteins is still present in hippocampus but on lower level. DG (dentate gyrus), PoDG (polymorph layer of the dentate gyrus).
Techniques Used: Expressing, Cell Culture, Negative Control

Figure Legend Snippet: ( A ) Immunocytochemical analysis of primary cultures of the dorsal thalamus using Ca V 1.2- (red), AKAP150- (green) and PKARIIβ- (blue) specific antibodies. Merged picture shows the close connection of the components of the proposed ternary complex, especially in somatic regions and proximal dendrites. Enlarged inlay represents a magnification of the area indicated by the rectangle. Data shown are representative pictures from several independent immunostainings and preparations of neurons. In all cases, omission of primary antibodies resulted without signal (negative control). Western blot analysis and pull down assays were done as described in " ". ( B ) Interaction of AKAP7-MBP and PKARIIβ-c-myc was detected using antibodies against c-myc. ( C ) IP of PKARIIβ-c-myc and AKAP5-GFP detected after incubation with GFP-coupled magnetic beads using antibodies derived against PKARIIβ. ( D ) Existence of PKA holoenzyme consisting of PKARIIβ-GST and PKAcsβ-GFP was detected with antibodies against GFP protein.
Techniques Used: Negative Control, Western Blot, Incubation, Magnetic Beads, Derivative Assay

Figure Legend Snippet: ( A ) Representative current traces recorded under control conditions (upper left panel) and in the presence of okadaic acid (OA) (10 µM; left down panel). The bar graph (right panel) represents D inact under different recording conditions (as indicated). The mean value of three cells recorded under control conditions was taken for comparison with three cells recorded under 10 µM OA. ( B ) Representative current traces recorded under control conditions (upper left panel) and in the presence of the isoproterenol alone (10 µM; left middle panel) or in combination with OA (10 µM; lower left panel). The bar graph (right panel) represents D inact under different recording conditions (as indicated). The mean value of five cells recorded under control conditions was taken for comparison with five cells recorded under 10 µM isoproterenol and five cells recorded under isoproterenol plus OA. Data are presented as means ± SEM of several independent experiments. *** P <0.001. Significance of isoproterenol plus OA (n = 5) versus controls (n = 5) was calculated by Student's t test. ** P <0.01. Significance of isoproterenol (n = 5) versus isoproterenol plus OA (n = 5) was calculated by Student's t test. The degree of inactivation is given by the normalized current amplitude of the mean postpulse I/V at +10 mV. ( C ) Immunocytochemical analysis of primary cultures of the dorsal thalamus using Ca V 1.2- (left panel, green) and PP2A-specific antibodies (right panel, red). Merge picture showed close association of the two proteins, especially in somatic regions and proximal dendrites. Data shown are representative pictures from several independent immunostainings and thalamic neurons preparations. In all cases, omission of primary antibodies resulted in no fluorescence signal above background (negative control).
Techniques Used: Fluorescence, Negative Control
ca v 1 2 (Alomone Labs)


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Ca V 1 2, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ca v 1 2/product/Alomone Labs
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
1) Product Images from "Tumor Necrosis Factor Alpha Inhibits L-Type Ca 2+ Channels in Sensitized Guinea Pig Airway Smooth Muscle through ERK 1/2 Pathway"
Article Title: Tumor Necrosis Factor Alpha Inhibits L-Type Ca 2+ Channels in Sensitized Guinea Pig Airway Smooth Muscle through ERK 1/2 Pathway
Journal: Mediators of Inflammation
doi: 10.1155/2016/5972302
Figure Legend Snippet: Sequence of primers and PCR conditions for the different subunits of L-VDCC.
Techniques Used: Sequencing, Amplification

Figure Legend Snippet: Detection of mRNA for L-VDCC subunits in guinea pig tracheal smooth muscle, as revealed by RT-PCR. (a) In airway smooth muscle, the PCR products at 470 and 459 bp length correspond to Ca V 1.2 and Ca V 1.3 cDNA, respectively. In this tissue, Ca V 1.1 and Ca V 1.4 were not found. Positive controls for these subunits were skeletal muscle (SKM, ~500 bp) and retina (~200 bp). Lane at the left corresponds to 1 Kb Plus DNA Ladder. (b) Representative PCR blots for Ca V 1.2 and Ca V 1.3 from nonsensitized (NS, n = 3) and sensitized (S, n = 4) smooth muscles. The first column in each blot corresponds to a negative control without template. The lower panel displays constitutive cDNA of GAPDH. (c) Densitometry data analysis for mRNA from Ca V 1.2 and Ca V 1.3 subunits showing no statistical significance between NS and S. Bars correspond to mean ± SEM.
Techniques Used: Reverse Transcription Polymerase Chain Reaction, Negative Control

Figure Legend Snippet: Immunofluorescence for Ca V 1.2 in nonsensitized and sensitized guinea pig tracheal smooth muscle. The first column shows immunoreactivity for Ca V 1.2 (stained green) in nonsensitized (a) and sensitized tissues (e); notice that Ca V 1.2 is located in the airway smooth muscle (SM) and epithelium (EPI, pointed by arrow); blocking peptide completely eliminated the fluorescence (i). The second and the third columns illustrate smooth muscle α -actin (stained red; (b), (f), (j)) and cell nuclei (DAPI, stained blue; (c), (g), (k)). The last column depicts merged images of the former three columns ((d), (h), (l)). In these merged images, Ca V 1.2 is seen to be colocalized with α -actin (stained yellow) on the smooth muscle.
Techniques Used: Immunofluorescence, Staining, Blocking Assay, Fluorescence
ca v 1 3 (Alomone Labs)


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Ca V 1 3, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ca v 1 3/product/Alomone Labs
Average 96 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
1) Product Images from "Tumor Necrosis Factor Alpha Inhibits L-Type Ca 2+ Channels in Sensitized Guinea Pig Airway Smooth Muscle through ERK 1/2 Pathway"
Article Title: Tumor Necrosis Factor Alpha Inhibits L-Type Ca 2+ Channels in Sensitized Guinea Pig Airway Smooth Muscle through ERK 1/2 Pathway
Journal: Mediators of Inflammation
doi: 10.1155/2016/5972302
Figure Legend Snippet: Sequence of primers and PCR conditions for the different subunits of L-VDCC.
Techniques Used: Sequencing, Amplification

Figure Legend Snippet: Detection of mRNA for L-VDCC subunits in guinea pig tracheal smooth muscle, as revealed by RT-PCR. (a) In airway smooth muscle, the PCR products at 470 and 459 bp length correspond to Ca V 1.2 and Ca V 1.3 cDNA, respectively. In this tissue, Ca V 1.1 and Ca V 1.4 were not found. Positive controls for these subunits were skeletal muscle (SKM, ~500 bp) and retina (~200 bp). Lane at the left corresponds to 1 Kb Plus DNA Ladder. (b) Representative PCR blots for Ca V 1.2 and Ca V 1.3 from nonsensitized (NS, n = 3) and sensitized (S, n = 4) smooth muscles. The first column in each blot corresponds to a negative control without template. The lower panel displays constitutive cDNA of GAPDH. (c) Densitometry data analysis for mRNA from Ca V 1.2 and Ca V 1.3 subunits showing no statistical significance between NS and S. Bars correspond to mean ± SEM.
Techniques Used: Reverse Transcription Polymerase Chain Reaction, Negative Control

Figure Legend Snippet: Immunofluorescence for Ca V 1.3 in nonsensitized and sensitized guinea pig tracheal smooth muscle. The first column shows immunoreactivity for Ca V 1.3 (stained green) in nonsensitized (a) and sensitized tissues (e); notice that Ca V 1.3 is located in the airway smooth muscle (SM) and epithelium (EPI, pointed by arrows); blocking peptide completely eliminated the fluorescence (i). The second and the third columns illustrate smooth muscle α -actin (stained red; (b), (f), (j)) and cell nuclei (DAPI, stained blue; (c), (g), (k)). The last column depicts merged images of the former three columns ((d), (h), (l)). In these merged images, Ca V 1.3 is seen to be colocalized with α -actin (stained yellow) on the smooth muscle.
Techniques Used: Immunofluorescence, Staining, Blocking Assay, Fluorescence
ca v 1 2 antibody (Alomone Labs)


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Ca V 1 2 Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ca v 1 2 antibody/product/Alomone Labs
Average 85 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
1) Product Images from "PI3Ks Maintain the Structural Integrity of T-Tubules in Cardiac Myocytes"
Article Title: PI3Ks Maintain the Structural Integrity of T-Tubules in Cardiac Myocytes
Journal: PLoS ONE
doi: 10.1371/journal.pone.0024404

Figure Legend Snippet: ( a ) Confocal microscopy images of myocytes from control and dKO mice colabeled with antibodies against Ca V 1.2 (green) and RyR (red). ( b ) Pearson's coefficient for colocalization of Ca V 1.2 and RyR ( n = 19 cells from ≥3 hearts per group). Data shown are mean ± SEM (* P = 8.1×10 −9 , t -test). 1D Fourier analysis of spatial frequency for Ca V 1.2 ( c ) and RyR ( d ) along the longitudinal axis of representative myocytes (averaged value from >10 lines). ( e ) The normalized output of 1D Fourier transform analysis from 19–20 cells for each group. Data shown are mean ± SEM (* P = 0.0001 (Ca V 1.2) and 0.015 (RyR), t -test). ( f ) Representative ind-dKO and ind-control myocytes colabeled with antibodies against Ca V 1.2 (green) and RyR (red). ( g ) Pearson's coefficient for colocalization of Ca V 1.2 and RyR ( n = 13–16 cells from ≥3 hearts per group). Data shown are mean ± SEM (* P = 0.016, t -test). 1D Fourier analysis of spatial frequency for Ca V 1.2 ( h ) and RyR ( i ) along the longitudinal axis of representative myocytes (averaged value from >10 lines). ( j ) The normalized output of 1D Fourier transform analysis from 19 cells for each group. Data shown are mean ± SEM (* P = 9.8×10 −6 (Ca V 1.2) and 4.3×10 −7 (RyR), t -test).
Techniques Used: Confocal Microscopy