anti brutp  (Roche)


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    Structured Review

    Roche anti brutp
    (A) NF-Y is associated with active transcription sites in living cells. After in vivo incorporation of <t>BrUTP</t> (run-on), cells were fixed and endogenous NF-YA (ii) and nascent RNA transcripts (i) were detected by indirect immunofluorescence combined with Confocal Scanning Laser Microscopy by using anti-NF-YA and anti-BrU antibodies. In the overlay (iii), yellow indicates colocalizations between NF-YA (green) and transcription sites (red). In panels vi and x cells were immunostained with anti-NF-YA, in panels v and ix with <t>anti-RPII</t> CTD repeat YSPTSPS (phospho S2) and anti-total RPII respectively. The majority of NF-YA (red) colocalizes with the activated form of RPII (green)(vii). (B) Cells were immunostained with anti-NF-YA (vii-xii), -acetylated H3K9 (i), -acetylated H4 (ii), -tri-methylated H3K9 (iii), -di-methylated H3K27 (iv), -tri-methylated H4K20 (v) and -NF-YB (vi) antibodies. The majority of NF-YA colocalizes with acetylated (xiii, xiv), but poor colocalization occurs with methylated histones (xv, xvi,xvii). Panel xviii shows the overlay of two subunits of NF-Y, NF-YA (xii) and NF-YB (vi). Panels from xix to xxiv represent a typical optical field of the merge. In figure 1A and 1B confocal analysis of single optical section is shown. The images have been collected with a 60x objective.
    Anti Brutp, supplied by Roche, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "NF-Y Dependent Epigenetic Modifications Discriminate between Proliferating and Postmitotic Tissue"

    Article Title: NF-Y Dependent Epigenetic Modifications Discriminate between Proliferating and Postmitotic Tissue

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0002047

    (A) NF-Y is associated with active transcription sites in living cells. After in vivo incorporation of BrUTP (run-on), cells were fixed and endogenous NF-YA (ii) and nascent RNA transcripts (i) were detected by indirect immunofluorescence combined with Confocal Scanning Laser Microscopy by using anti-NF-YA and anti-BrU antibodies. In the overlay (iii), yellow indicates colocalizations between NF-YA (green) and transcription sites (red). In panels vi and x cells were immunostained with anti-NF-YA, in panels v and ix with anti-RPII CTD repeat YSPTSPS (phospho S2) and anti-total RPII respectively. The majority of NF-YA (red) colocalizes with the activated form of RPII (green)(vii). (B) Cells were immunostained with anti-NF-YA (vii-xii), -acetylated H3K9 (i), -acetylated H4 (ii), -tri-methylated H3K9 (iii), -di-methylated H3K27 (iv), -tri-methylated H4K20 (v) and -NF-YB (vi) antibodies. The majority of NF-YA colocalizes with acetylated (xiii, xiv), but poor colocalization occurs with methylated histones (xv, xvi,xvii). Panel xviii shows the overlay of two subunits of NF-Y, NF-YA (xii) and NF-YB (vi). Panels from xix to xxiv represent a typical optical field of the merge. In figure 1A and 1B confocal analysis of single optical section is shown. The images have been collected with a 60x objective.
    Figure Legend Snippet: (A) NF-Y is associated with active transcription sites in living cells. After in vivo incorporation of BrUTP (run-on), cells were fixed and endogenous NF-YA (ii) and nascent RNA transcripts (i) were detected by indirect immunofluorescence combined with Confocal Scanning Laser Microscopy by using anti-NF-YA and anti-BrU antibodies. In the overlay (iii), yellow indicates colocalizations between NF-YA (green) and transcription sites (red). In panels vi and x cells were immunostained with anti-NF-YA, in panels v and ix with anti-RPII CTD repeat YSPTSPS (phospho S2) and anti-total RPII respectively. The majority of NF-YA (red) colocalizes with the activated form of RPII (green)(vii). (B) Cells were immunostained with anti-NF-YA (vii-xii), -acetylated H3K9 (i), -acetylated H4 (ii), -tri-methylated H3K9 (iii), -di-methylated H3K27 (iv), -tri-methylated H4K20 (v) and -NF-YB (vi) antibodies. The majority of NF-YA colocalizes with acetylated (xiii, xiv), but poor colocalization occurs with methylated histones (xv, xvi,xvii). Panel xviii shows the overlay of two subunits of NF-Y, NF-YA (xii) and NF-YB (vi). Panels from xix to xxiv represent a typical optical field of the merge. In figure 1A and 1B confocal analysis of single optical section is shown. The images have been collected with a 60x objective.

    Techniques Used: In Vivo, Immunofluorescence, Microscopy, Methylation

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    Incubation:

    Article Title: The Extrachromosomal EAST Protein of Drosophila Can Associate with Polytene Chromosomes and Regulate Gene Expression
    Article Snippet: The labeling of nascent RNA was adapted from a published protocol . .. In brief, larval salivary glands were dissected in Ringer's solution, incubated for 15 minutes in detergent buffer supplemented with 0.2 U/µl RNase inhibitor (RNasin, Promega), 2 mM ATP, 1 mM CTP, 1 mM GTP (Roche) and 1 mM BrUTP (Sigma) and fixed in DB-fixative. .. BrUTP incorporated into nascent RNA was detected by indirect immunofluorescence using a monoclonal anti-BrUTP antibody (MAB3424, Chemicon).

    Article Title: Histone Acetylase Inhibitor Curcumin Impairs Mouse Spermiogenesis-An In Vitro Study
    Article Snippet: The Curcumin treated cells were washed in physiological buffer (PB) [100 mM potassium acetate, 30 mM KCl, 1 mM MgCl2 , 10 mM Na2 HPO4 , 1 mM ATP supplemented with 1 mM dithiothreitol (DTT), 0.2 mM phenylmethylsulfonyl fluoride (PMSF), and 50 units/ml of RNase inhibitor (Promega)], then transferred to transport buffer (TB) [100 mM potassium acetate, 1 mM MnCl2 , 50 mM (NH4 )2 SO4 , 30 mM KCl, 10 mM Na2 HPO4 containing 2 mM ATP, 0.4 mM each of guanosine triphosphate (GTP), cytidine triphosphate (CTP), bromouridine triphosphate (BrUTP), and 1 mM MgCl2 ], incubated at 34°C for 30 min. After washed 3 times with PBS, the cells were fixed with 4% PFA for 1 h, smeared on the slides and air-dried. .. To detect BrUTP incorporation, the slides were incubated with 2 µg/ml anti-BrUTP antibody (Roche) overnight, followed with 0.5 mg/ml CY3-conjugated anti-mouse IgG antibody plus 10 µg/ml Alexa Fluor® 488 conjugated-peanut agglutinin (PNA)(Molecular Probe) , for 1 h. After counterstained with Hoechst 33342, the results were examined under a fluorescence microscope (Nikon E600). ..

    Article Title: Poly(A) RNAs Including Coding Proteins RNAs Occur in Plant Cajal Bodies
    Article Snippet: Immunodetection of 5-bromouracil incorporated and poly(A) RNA After 3–4 days of culture, lupine seedlings were transferred to Petri dishes with 5-bromouarcil in tap water (20 mM, Sigma-Aldrich) soaked tissue paper for 2, 4, 5 or 7 h. The roots were fixed and sectioned, and the sections were treated according to the above protocol. .. Then sections were incubated with anti-BrUTP antibodies (F. Hoffmann-LaRoche Ltd., Rotkreuz, Switzerland) (diluted 1∶1000) overnight at 4°C, then washed in PBS and incubated with goat anti-mouse antibodies labelled with Alexa 488 (Molecular Probes, NY, USA). .. Next, in situ hybridisation of poly(A) RNA was performed according to the protocol described for double localisation with poly(A) RNA.

    Fluorescence:

    Article Title: Histone Acetylase Inhibitor Curcumin Impairs Mouse Spermiogenesis-An In Vitro Study
    Article Snippet: The Curcumin treated cells were washed in physiological buffer (PB) [100 mM potassium acetate, 30 mM KCl, 1 mM MgCl2 , 10 mM Na2 HPO4 , 1 mM ATP supplemented with 1 mM dithiothreitol (DTT), 0.2 mM phenylmethylsulfonyl fluoride (PMSF), and 50 units/ml of RNase inhibitor (Promega)], then transferred to transport buffer (TB) [100 mM potassium acetate, 1 mM MnCl2 , 50 mM (NH4 )2 SO4 , 30 mM KCl, 10 mM Na2 HPO4 containing 2 mM ATP, 0.4 mM each of guanosine triphosphate (GTP), cytidine triphosphate (CTP), bromouridine triphosphate (BrUTP), and 1 mM MgCl2 ], incubated at 34°C for 30 min. After washed 3 times with PBS, the cells were fixed with 4% PFA for 1 h, smeared on the slides and air-dried. .. To detect BrUTP incorporation, the slides were incubated with 2 µg/ml anti-BrUTP antibody (Roche) overnight, followed with 0.5 mg/ml CY3-conjugated anti-mouse IgG antibody plus 10 µg/ml Alexa Fluor® 488 conjugated-peanut agglutinin (PNA)(Molecular Probe) , for 1 h. After counterstained with Hoechst 33342, the results were examined under a fluorescence microscope (Nikon E600). ..

    Microscopy:

    Article Title: Histone Acetylase Inhibitor Curcumin Impairs Mouse Spermiogenesis-An In Vitro Study
    Article Snippet: The Curcumin treated cells were washed in physiological buffer (PB) [100 mM potassium acetate, 30 mM KCl, 1 mM MgCl2 , 10 mM Na2 HPO4 , 1 mM ATP supplemented with 1 mM dithiothreitol (DTT), 0.2 mM phenylmethylsulfonyl fluoride (PMSF), and 50 units/ml of RNase inhibitor (Promega)], then transferred to transport buffer (TB) [100 mM potassium acetate, 1 mM MnCl2 , 50 mM (NH4 )2 SO4 , 30 mM KCl, 10 mM Na2 HPO4 containing 2 mM ATP, 0.4 mM each of guanosine triphosphate (GTP), cytidine triphosphate (CTP), bromouridine triphosphate (BrUTP), and 1 mM MgCl2 ], incubated at 34°C for 30 min. After washed 3 times with PBS, the cells were fixed with 4% PFA for 1 h, smeared on the slides and air-dried. .. To detect BrUTP incorporation, the slides were incubated with 2 µg/ml anti-BrUTP antibody (Roche) overnight, followed with 0.5 mg/ml CY3-conjugated anti-mouse IgG antibody plus 10 µg/ml Alexa Fluor® 488 conjugated-peanut agglutinin (PNA)(Molecular Probe) , for 1 h. After counterstained with Hoechst 33342, the results were examined under a fluorescence microscope (Nikon E600). ..

    other:

    Article Title: UBF-binding site arrays form pseudo-NORs and sequester the RNA polymerase I transcription machinery
    Article Snippet: BrUTP was visualized with anti-BrdUTP antibodies (Roche) combined with FITC-conjugated secondary antibodies.

    Article Title: UBF-binding site arrays form pseudo-NORs and sequester the RNA polymerase I transcription machinery
    Article Snippet: Incorporated BrUTP was visualized with anti-BrdUTP antibodies (Roche) combined with FITC-conjugated secondary antibodies.

    Labeling:

    Article Title: Blocking Variant Surface Glycoprotein Synthesis in Trypanosoma brucei Triggers a General Arrest in Translation Initiation
    Article Snippet: Microscopy Labeling of nascent RNA using BrUTP was performed essentially according to , only the Saponin (Sigma) incubation was performed at 4°C. .. After the transcription reaction, cells were washed and fixed in 2% paraformaldehyde and BrUTP labeled transcripts were detected with a monoclonal anti-BrdUTP antibody (Roche). .. For transmission electron microscopy analysis of membrane bound ribosomes, cells were fixed by addition of 2.5% glutaraldehyde directly to the culture medium followed by fixation in 2.5% glutaraldehyde in 0.1 M phosphate buffer pH 7.0 for 2 hours at room temperature without osmium tetroxide post-fixation.

    Article Title: Nucleolar Assembly of the Rrna Processing Machinery in Living Cells
    Article Snippet: Assay of RNA Pol I Activity In Situ (Run On) The run on assay was performed on HeLa cells grown as monolayers, essentially as described ( ) in conditions set up to reveal RNA pol I transcription ( ). .. BrUTP incorporation was detected by immunofluorescence labeling using a mouse anti-bromodeoxyuridine monoclonal antibody (Roche) revealed by FITC-conjugated goat anti–mouse antibodies (Jackson ImmunoResearch Laboratories). .. Immunoblotting For protein analysis, control HeLa cells, fibrillarin-GFP, and Nop52-GFP transfected HeLa cell lines were solubilized in SDS sample buffer and analyzed by 10% SDS-PAGE.

    Immunofluorescence:

    Article Title: Nucleolar Assembly of the Rrna Processing Machinery in Living Cells
    Article Snippet: Assay of RNA Pol I Activity In Situ (Run On) The run on assay was performed on HeLa cells grown as monolayers, essentially as described ( ) in conditions set up to reveal RNA pol I transcription ( ). .. BrUTP incorporation was detected by immunofluorescence labeling using a mouse anti-bromodeoxyuridine monoclonal antibody (Roche) revealed by FITC-conjugated goat anti–mouse antibodies (Jackson ImmunoResearch Laboratories). .. Immunoblotting For protein analysis, control HeLa cells, fibrillarin-GFP, and Nop52-GFP transfected HeLa cell lines were solubilized in SDS sample buffer and analyzed by 10% SDS-PAGE.

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    Roche anti brutp
    (A) NF-Y is associated with active transcription sites in living cells. After in vivo incorporation of <t>BrUTP</t> (run-on), cells were fixed and endogenous NF-YA (ii) and nascent RNA transcripts (i) were detected by indirect immunofluorescence combined with Confocal Scanning Laser Microscopy by using anti-NF-YA and anti-BrU antibodies. In the overlay (iii), yellow indicates colocalizations between NF-YA (green) and transcription sites (red). In panels vi and x cells were immunostained with anti-NF-YA, in panels v and ix with <t>anti-RPII</t> CTD repeat YSPTSPS (phospho S2) and anti-total RPII respectively. The majority of NF-YA (red) colocalizes with the activated form of RPII (green)(vii). (B) Cells were immunostained with anti-NF-YA (vii-xii), -acetylated H3K9 (i), -acetylated H4 (ii), -tri-methylated H3K9 (iii), -di-methylated H3K27 (iv), -tri-methylated H4K20 (v) and -NF-YB (vi) antibodies. The majority of NF-YA colocalizes with acetylated (xiii, xiv), but poor colocalization occurs with methylated histones (xv, xvi,xvii). Panel xviii shows the overlay of two subunits of NF-Y, NF-YA (xii) and NF-YB (vi). Panels from xix to xxiv represent a typical optical field of the merge. In figure 1A and 1B confocal analysis of single optical section is shown. The images have been collected with a 60x objective.
    Anti Brutp, supplied by Roche, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti brutp/product/Roche
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti brutp - by Bioz Stars, 2021-03
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    97
    Roche mab against brutp
    (A) NF-Y is associated with active transcription sites in living cells. After in vivo incorporation of <t>BrUTP</t> (run-on), cells were fixed and endogenous NF-YA (ii) and nascent RNA transcripts (i) were detected by indirect immunofluorescence combined with Confocal Scanning Laser Microscopy by using anti-NF-YA and anti-BrU antibodies. In the overlay (iii), yellow indicates colocalizations between NF-YA (green) and transcription sites (red). In panels vi and x cells were immunostained with anti-NF-YA, in panels v and ix with <t>anti-RPII</t> CTD repeat YSPTSPS (phospho S2) and anti-total RPII respectively. The majority of NF-YA (red) colocalizes with the activated form of RPII (green)(vii). (B) Cells were immunostained with anti-NF-YA (vii-xii), -acetylated H3K9 (i), -acetylated H4 (ii), -tri-methylated H3K9 (iii), -di-methylated H3K27 (iv), -tri-methylated H4K20 (v) and -NF-YB (vi) antibodies. The majority of NF-YA colocalizes with acetylated (xiii, xiv), but poor colocalization occurs with methylated histones (xv, xvi,xvii). Panel xviii shows the overlay of two subunits of NF-Y, NF-YA (xii) and NF-YB (vi). Panels from xix to xxiv represent a typical optical field of the merge. In figure 1A and 1B confocal analysis of single optical section is shown. The images have been collected with a 60x objective.
    Mab Against Brutp, supplied by Roche, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mab against brutp/product/Roche
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mab against brutp - by Bioz Stars, 2021-03
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    99
    Roche anti brutp antibody
    (A) NF-Y is associated with active transcription sites in living cells. After in vivo incorporation of <t>BrUTP</t> (run-on), cells were fixed and endogenous NF-YA (ii) and nascent RNA transcripts (i) were detected by indirect immunofluorescence combined with Confocal Scanning Laser Microscopy by using anti-NF-YA and anti-BrU antibodies. In the overlay (iii), yellow indicates colocalizations between NF-YA (green) and transcription sites (red). In panels vi and x cells were immunostained with anti-NF-YA, in panels v and ix with <t>anti-RPII</t> CTD repeat YSPTSPS (phospho S2) and anti-total RPII respectively. The majority of NF-YA (red) colocalizes with the activated form of RPII (green)(vii). (B) Cells were immunostained with anti-NF-YA (vii-xii), -acetylated H3K9 (i), -acetylated H4 (ii), -tri-methylated H3K9 (iii), -di-methylated H3K27 (iv), -tri-methylated H4K20 (v) and -NF-YB (vi) antibodies. The majority of NF-YA colocalizes with acetylated (xiii, xiv), but poor colocalization occurs with methylated histones (xv, xvi,xvii). Panel xviii shows the overlay of two subunits of NF-Y, NF-YA (xii) and NF-YB (vi). Panels from xix to xxiv represent a typical optical field of the merge. In figure 1A and 1B confocal analysis of single optical section is shown. The images have been collected with a 60x objective.
    Anti Brutp Antibody, supplied by Roche, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti brutp antibody/product/Roche
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti brutp antibody - by Bioz Stars, 2021-03
    99/100 stars
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    Image Search Results


    (A) NF-Y is associated with active transcription sites in living cells. After in vivo incorporation of BrUTP (run-on), cells were fixed and endogenous NF-YA (ii) and nascent RNA transcripts (i) were detected by indirect immunofluorescence combined with Confocal Scanning Laser Microscopy by using anti-NF-YA and anti-BrU antibodies. In the overlay (iii), yellow indicates colocalizations between NF-YA (green) and transcription sites (red). In panels vi and x cells were immunostained with anti-NF-YA, in panels v and ix with anti-RPII CTD repeat YSPTSPS (phospho S2) and anti-total RPII respectively. The majority of NF-YA (red) colocalizes with the activated form of RPII (green)(vii). (B) Cells were immunostained with anti-NF-YA (vii-xii), -acetylated H3K9 (i), -acetylated H4 (ii), -tri-methylated H3K9 (iii), -di-methylated H3K27 (iv), -tri-methylated H4K20 (v) and -NF-YB (vi) antibodies. The majority of NF-YA colocalizes with acetylated (xiii, xiv), but poor colocalization occurs with methylated histones (xv, xvi,xvii). Panel xviii shows the overlay of two subunits of NF-Y, NF-YA (xii) and NF-YB (vi). Panels from xix to xxiv represent a typical optical field of the merge. In figure 1A and 1B confocal analysis of single optical section is shown. The images have been collected with a 60x objective.

    Journal: PLoS ONE

    Article Title: NF-Y Dependent Epigenetic Modifications Discriminate between Proliferating and Postmitotic Tissue

    doi: 10.1371/journal.pone.0002047

    Figure Lengend Snippet: (A) NF-Y is associated with active transcription sites in living cells. After in vivo incorporation of BrUTP (run-on), cells were fixed and endogenous NF-YA (ii) and nascent RNA transcripts (i) were detected by indirect immunofluorescence combined with Confocal Scanning Laser Microscopy by using anti-NF-YA and anti-BrU antibodies. In the overlay (iii), yellow indicates colocalizations between NF-YA (green) and transcription sites (red). In panels vi and x cells were immunostained with anti-NF-YA, in panels v and ix with anti-RPII CTD repeat YSPTSPS (phospho S2) and anti-total RPII respectively. The majority of NF-YA (red) colocalizes with the activated form of RPII (green)(vii). (B) Cells were immunostained with anti-NF-YA (vii-xii), -acetylated H3K9 (i), -acetylated H4 (ii), -tri-methylated H3K9 (iii), -di-methylated H3K27 (iv), -tri-methylated H4K20 (v) and -NF-YB (vi) antibodies. The majority of NF-YA colocalizes with acetylated (xiii, xiv), but poor colocalization occurs with methylated histones (xv, xvi,xvii). Panel xviii shows the overlay of two subunits of NF-Y, NF-YA (xii) and NF-YB (vi). Panels from xix to xxiv represent a typical optical field of the merge. In figure 1A and 1B confocal analysis of single optical section is shown. The images have been collected with a 60x objective.

    Article Snippet: The following primary antibodies (diluted in 1% BSA) were used: anti-NF-YA rabbit polyclonal (on run on experiments) (Rockland n°200-401-100, Gilbertsville, PA), anti-NF-YA mouse monoclonal from hybridoma cells, anti-NF-YB rabbit polyclonal (gifts from R. Mantovani), anti-BrUTP (Roche Diagnostics n°1170376, clone BMC 9318), anti-RPII (Santa Cruz n° 899), anti- RPIIS2 (Abcam n°5095), anti-PAN-H4ac (Upstate n°06-598), anti-H3ac-k9 (Abcam n°4441), anti-H3tri-m-k9 (Abcam n°8898), anti-H4tri-m-k20 (Abcam n°9053), anti-p300 (Santa Cruz n° 584 and 585), anti-H3di-m-k27 (Abcam n°24684) and anti-HDAC1 (Sigma H3284).

    Techniques: In Vivo, Immunofluorescence, Microscopy, Methylation