anti brutp  (Millipore)

 
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    Name:
    Monoclonal Anti BrdU antibody
    Description:
    5 Bromo 2 deoxyuridine BrdU is a carcinogen that may be absorbed by skin or inhalation BrdU solutions are sensitive to light Monoclonal anti BrdU immunohistology grade mouse IgG1 isotype is derived from the BU 33 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB c mice immunized with bromodeoxyuridine conjugated to KLH
    Catalog Number:
    b2531
    Price:
    None
    Applications:
    Monoclonal Anti-BrdU antibody has been used in immunostaining, BrdU incorporation into DNA, immunohistochemistry and uterine labeling index. It has also been used to investigate cell proliferation.
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    Structured Review

    Millipore anti brutp
    Monoclonal Anti BrdU antibody
    5 Bromo 2 deoxyuridine BrdU is a carcinogen that may be absorbed by skin or inhalation BrdU solutions are sensitive to light Monoclonal anti BrdU immunohistology grade mouse IgG1 isotype is derived from the BU 33 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB c mice immunized with bromodeoxyuridine conjugated to KLH
    https://www.bioz.com/result/anti brutp/product/Millipore
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti brutp - by Bioz Stars, 2021-03
    94/100 stars

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    Related Articles

    other:

    Article Title: Signaling Cross Talk between Growth Hormone (GH) and Insulin-Like Growth Factor-I (IGF-I) in Pancreatic Islet ?-Cells
    Article Snippet: Anti-IGF-IRβ (Santa Cruz Biotechnology, Santa Cruz, CA), monoclonal anti-pTyr clone 4G10 (Millipore/Upstate Biotechnology, Lake Placid, NY), anti-pSTAT5 (Invitrogen, Carlsbad, CA), anti-pAkt (Cell Signaling, Beverly, MA), anti-Akt (Cell Signaling), anti-β-actin (Sigma), and the monoclonal anti-BrdU clone BU-33 (Sigma) antibodies were commercially available.

    Incubation:

    Article Title: Targeting Wnt/β-Catenin Activated Cells with Dominant-Negative N-cadherin to Reduce Neointima Formation
    Article Snippet: The cells were fixed after 24 hr with ice-cold methanol for 5 min. Hydrochloric (HCl) acid (4N) was added to each well and incubated for 30 min at 37°C. .. VSMCs were then incubated with 1 μM mouse anti-BrdU, (Sigma, B2531) in 1% (v/v) goat serum and 1% (w/v) BSA/PBS at 4°C overnight. .. Cells were then incubated with biotinylated goat anti-mouse antibody diluted 1:200 in 1% BSA/PBS for 30 min at room temperature and then Extravidin-HRP diluted 1:200 in 1% BSA/PBS for 30 min at room temperature.

    Article Title: Properties of astrocytes cultured from GFAP over-expressing and GFAP mutant mice
    Article Snippet: The cells were fixed with ice cold methanol for 10 min., rinsed three times in PBS, rehydrated in PBS overnight, incubated in 2N HCl /PBS for 20 min at RT, and then rinsed three times in PBS again. .. After treatment with blocking buffer (5% donkey serum, 0.2% Triton X-100 in PBS) for 1 hour at RT, the cells were then incubated with polyclonal anti-GFAP (1:1000, DAKO) and monoclonal anti-BrdU (1:1000, Sigma) antibodies in 1% donkey serum / PBS, overnight at 4°C. .. After rinses in PBS, Alexa 488 and 594 secondary antibodies (all at 1:500 dilutions, Molecular Probes) were applied for 1 hour at RT.

    Immunohistochemistry:

    Article Title: MARVELD1 depletion leads to dysfunction of motor and cognition via regulating glia-dependent neuronal migration during brain development
    Article Snippet: Brains were either embedded in paraffin and sectioned to 4–5- μm sections or incubated with a high concentration of sucrose (30%) and rapidly frozen and sectioned at 8 μm. .. For immunohistochemistry and immunofluorescence, the following primary antibodies were used: anti-Calb (1:200, Sigma, no.sab4200543), anti-Blbp (1:100, Abcam, no.ab32423), anti-GFAP (1:200, Abcam, no.ab7260 or ab10062), anti-ITGB1 (1:100, Abcam, no.ab183666 or ab95623), anti-FAK (1:100, Abcam, no.ab40794), anti-p397-FAK (1:100, Abcam, no.ab81298), anti-BrdU (1:100, Sigma, no.b2531), anti-MARVELD1 (1:100, Abcam, no.ab91640 or no.ab169184) and anti-NeuN (1:200, Abcam, no.ab104224). .. In immunohistochemistry study, paraffin-embedded sections were incubated in 3% H2 O2 after microwave antigen retrieval.

    Immunofluorescence:

    Article Title: MARVELD1 depletion leads to dysfunction of motor and cognition via regulating glia-dependent neuronal migration during brain development
    Article Snippet: Brains were either embedded in paraffin and sectioned to 4–5- μm sections or incubated with a high concentration of sucrose (30%) and rapidly frozen and sectioned at 8 μm. .. For immunohistochemistry and immunofluorescence, the following primary antibodies were used: anti-Calb (1:200, Sigma, no.sab4200543), anti-Blbp (1:100, Abcam, no.ab32423), anti-GFAP (1:200, Abcam, no.ab7260 or ab10062), anti-ITGB1 (1:100, Abcam, no.ab183666 or ab95623), anti-FAK (1:100, Abcam, no.ab40794), anti-p397-FAK (1:100, Abcam, no.ab81298), anti-BrdU (1:100, Sigma, no.b2531), anti-MARVELD1 (1:100, Abcam, no.ab91640 or no.ab169184) and anti-NeuN (1:200, Abcam, no.ab104224). .. In immunohistochemistry study, paraffin-embedded sections were incubated in 3% H2 O2 after microwave antigen retrieval.

    In Vivo:

    Article Title: BMP/SMAD Pathway Promotes Neurogenesis of Midbrain Dopaminergic Neurons In Vivo and in Human Induced Pluripotent and Neural Stem Cells
    Article Snippet: For DAT and GIRK2 staining, no permeabilization was needed and Triton X-100 was omitted in all solutions. .. Primary antibodies used for the in vivo study were as follows: mouse anti-SHH (1:10), mouse anti-NKX6.1 (1:10), mouse anti-ISLET1/2 (1:10), mouse anti-MSX1/2 (1:10), mouse anti-FOXA2 (1:10), mouse anti-NESTIN (1:10), mouse anti-EN1 (1:50) - all from DSHB, rabbit anti-LMX1A (1:400, catalog #AB10533; Millipore), rabbit anti-LMX1B (1:1000, gift from Dr. C. Birchmeier, MDC, Berlin), rabbit anti-NURR1 (1:100, catalog #SC991; Santa Cruz Biotechnology), mouse anti-NURR1 (1:100, catalog #376984; Santa Cruz Biotechnology) mouse anti-neurogenin-2 (1:800, catalog #MAB3314; R & D Systems), rabbit anti-TH (1:200, catalog #AB152; Millipore), mouse anti-TH (1:200, catalog #MAB318; Millipore), rabbit anti-phospho-SMAD1/5/8 (1:200, #9511S Cell Signaling), sheep anti-BMP5 (1:10, catalog #AF6176; R & D Systems), rabbit anti-phospho-histone-H3 (1:1000, catalog #06-570; Millipore), rabbit anti-phospho-P38 (1:1000, catalog #4511 Cell Signaling), rabbit anti-GIRK2 (1:200, catalog #APC-006; Alomone Laboratories), rabbit anti-SOX6 (1:500, catalog #ab30455; Abcam), mouse anti-POU4F1 (1:300, catalog #sc8429; Santa Cruz Biotechnology), mouse anti-N-cadherin (1:200, catalog #610920; BD Biosciences), rabbit anti-ZO1 (1:100, catalog #40-2200; Invitrogen), rabbit anti-KI67 (1:100, catalog # ab16667; Abcam), rabbit anti-cleaved-caspase 3 (1:100, catalog #3661; Cell Signaling Technology), rabbit anti-MAP-2 (1:200, catalog #sc20172; Santa Cruz Biotechnology), rabbit anti-β-catenin (1:400, catalog #9587s; Cell Signaling Technology), rabbit anti-SHH (1:50, catalog #sc9024; Santa Cruz Biotechnology), rabbit anti-BMPR1B (1:100, catalog #10537; Orbigen), rabbit anti-PITX3 (catalog #38-2850; Thermo Fisher Scientific), mouse anti-BrdU (catalog #B2531; Sigma-Aldrich), rabbit anti-CCND1(1:150, catalog #sc-450; Santa Cruz Biotechnology), rabbit anti-phospho-β-catenin (1:200, catalog #9561s; Cell Signaling Technology), rabbit anti-calbindin (1:500, catalog # d -28k; Swant), and rabbit anti-β III tubulin/TUJ1 (1:500, catalog #302 302; Synaptic Systems). .. For the in vitro study, the following primary antibodies were used: mouse anti-TH (1:1000, catalog #MAB318; Millipore), rabbit anti-TH (1:1000, catalog #ab112; Abcam), rat anti-DAT (1:50, catalog #sc-32259; SCBT), rabbit anti-LMX1A (1:1000, catalog # ab10533; Millipore), goat anti-GIRK2 (1:200, catalog #ab65096; Abcam), and rabbit anti-calbindin (1:1000, catalog #CB38; Swant).

    Blocking Assay:

    Article Title: Properties of astrocytes cultured from GFAP over-expressing and GFAP mutant mice
    Article Snippet: The cells were fixed with ice cold methanol for 10 min., rinsed three times in PBS, rehydrated in PBS overnight, incubated in 2N HCl /PBS for 20 min at RT, and then rinsed three times in PBS again. .. After treatment with blocking buffer (5% donkey serum, 0.2% Triton X-100 in PBS) for 1 hour at RT, the cells were then incubated with polyclonal anti-GFAP (1:1000, DAKO) and monoclonal anti-BrdU (1:1000, Sigma) antibodies in 1% donkey serum / PBS, overnight at 4°C. .. After rinses in PBS, Alexa 488 and 594 secondary antibodies (all at 1:500 dilutions, Molecular Probes) were applied for 1 hour at RT.

    Staining:

    Article Title: WW Domain Containing Transcription Regulator regulates human conjunctiva epithelial cell proliferation via inhibiting TGF? signaling pathway
    Article Snippet: .. After treatment with 2 N hydrochloric acid (HCL) for 15 min, cells were stained with monoclonal anti-BrdU (Sigma, St. Louis, MO) and polyclonal anti-TAZ (Santa Cruz Biotechnology, Santa Cruz, CA). .. 4',6-diamidino-2-phenylindole (DAPI) was applied in the mounting medium as a nucleus counter stain.

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  • 93
    Millipore mouse anti brutp primary antibody
    (Top). Localization of Br-RNA and dsRNA in typical PCV-infected BY-2 tobacco protoplasts. PCV-infected (A, B, and C) or mock-inoculated (D and E) protoplasts were treated with actinomycin D added at 17 hpi and then incubated for 1 h prior to addition of <t>BrUTP</t> and further incubated for 6 h. The protoplasts were then immunolabeled with mouse BrUTP-specific antibodies and dsRNA guinea pig antibodies, which were then detected with, respectively, <t>Alexa</t> 488 (A and D)- and Alexa 568 (B and E)-labeled secondary antibodies. BrUTP labeling is in green, and dsRNA labeling is in red. (C) Superimposition of the images to the left. The confocal images were collected with a focal depth of 0.45 μm. Bar, 10 μm.
    Mouse Anti Brutp Primary Antibody, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse anti brutp primary antibody/product/Millipore
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse anti brutp primary antibody - by Bioz Stars, 2021-03
    93/100 stars
      Buy from Supplier

    85
    Millipore monoclonal anti brutp antibody
    (Top). Localization of Br-RNA and dsRNA in typical PCV-infected BY-2 tobacco protoplasts. PCV-infected (A, B, and C) or mock-inoculated (D and E) protoplasts were treated with actinomycin D added at 17 hpi and then incubated for 1 h prior to addition of <t>BrUTP</t> and further incubated for 6 h. The protoplasts were then immunolabeled with mouse BrUTP-specific antibodies and dsRNA guinea pig antibodies, which were then detected with, respectively, <t>Alexa</t> 488 (A and D)- and Alexa 568 (B and E)-labeled secondary antibodies. BrUTP labeling is in green, and dsRNA labeling is in red. (C) Superimposition of the images to the left. The confocal images were collected with a focal depth of 0.45 μm. Bar, 10 μm.
    Monoclonal Anti Brutp Antibody, supplied by Millipore, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/monoclonal anti brutp antibody/product/Millipore
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    monoclonal anti brutp antibody - by Bioz Stars, 2021-03
    85/100 stars
      Buy from Supplier

    Image Search Results


    (Top). Localization of Br-RNA and dsRNA in typical PCV-infected BY-2 tobacco protoplasts. PCV-infected (A, B, and C) or mock-inoculated (D and E) protoplasts were treated with actinomycin D added at 17 hpi and then incubated for 1 h prior to addition of BrUTP and further incubated for 6 h. The protoplasts were then immunolabeled with mouse BrUTP-specific antibodies and dsRNA guinea pig antibodies, which were then detected with, respectively, Alexa 488 (A and D)- and Alexa 568 (B and E)-labeled secondary antibodies. BrUTP labeling is in green, and dsRNA labeling is in red. (C) Superimposition of the images to the left. The confocal images were collected with a focal depth of 0.45 μm. Bar, 10 μm.

    Journal: Journal of Virology

    Article Title: Intracellular Localization of the Peanut Clump Virus Replication Complex in Tobacco BY-2 Protoplasts Containing Green Fluorescent Protein-Labeled Endoplasmic Reticulum or Golgi Apparatus

    doi: 10.1128/JVI.76.2.865-874.2002

    Figure Lengend Snippet: (Top). Localization of Br-RNA and dsRNA in typical PCV-infected BY-2 tobacco protoplasts. PCV-infected (A, B, and C) or mock-inoculated (D and E) protoplasts were treated with actinomycin D added at 17 hpi and then incubated for 1 h prior to addition of BrUTP and further incubated for 6 h. The protoplasts were then immunolabeled with mouse BrUTP-specific antibodies and dsRNA guinea pig antibodies, which were then detected with, respectively, Alexa 488 (A and D)- and Alexa 568 (B and E)-labeled secondary antibodies. BrUTP labeling is in green, and dsRNA labeling is in red. (C) Superimposition of the images to the left. The confocal images were collected with a focal depth of 0.45 μm. Bar, 10 μm.

    Article Snippet: The protoplasts were then processed as for immunofluorescent detection of proteins by using mouse anti-BrUTP primary antibody (Sigma) and Alexa 488- or 568-conjugated secondary antibody to detect BrUTP incorporation or by using guinea pig polyclonal antibody to detect double-stranded RNA (dsRNA; gift from J. Y. Lee, Macfarlane Burnet Center for Medical Research, Fairfield, Australia) and Alexa 568 secondary antibody to detect dsRNA.

    Techniques: Infection, Incubation, Immunolabeling, Labeling