Structured Review

Abiocode Inc anti bri1
Anti Bri1, supplied by Abiocode Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti bri1/product/Abiocode Inc
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
anti bri1 - by Bioz Stars, 2024-04
86/100 stars

Images


Structured Review

Abiocode Inc anti bri1
Glucose influences the interactions between <t>BRI1</t> and BAK1. a Glucose regulates the physical interactions between BRI1 and BAK1. pBRI1:BRI1-GFP ; 35S:BAK1 - HA or 35S:BAK1-HA seedlings were grown in the light for 5 days, then incubated in darkness for 4 days, and treated with different concentrations of glucose (G) for 4 or 24 h. IN input; IP immunoprecipitation; IB immunoblot. b Quantification of BRI1-GFP and BAK1-HA associations in Arabidopsis seedlings treated with different concentrations of glucose (G) for 4 h from a . The ratio value of immunoprecipitated BAK1-HA to input BAK1-HA in response to 0% glucose was set at 1. Values for glucose treatments are given as mean ± SD ( n = 3). ** P < 0.01 compared with the value for 0% glucose treatment by Student’s t -test. c Glucose influences the phosphorylation levels of BRI1 in Arabidopsis. pBRI1:BRI1-GFP seedlings were grown in the light for 5 days, then incubated in darkness for 4 days, and treated with different concentrations of glucose (G) for 4 or 24 h. IN input; IP immunoprecipitation; IB immunoblot. d Quantification of BRI1-GFP phosphorylation in Arabidopsis seedlings treated with different concentrations of glucose for 4 h from c . The ratio value of phosphorylated BRI1-GFP to immunoprecipitated BRI1-GFP in response to 0% glucose was set at 1. Values for glucose treatments are given as mean ± SD ( n = 3). ** P < 0.01 compared with the value for 0% glucose treatment by Student’s t -test. e Glucose influences the phosphorylation levels of BAK1 in Arabidopsis. pBAK1:BAK1-GFP seedlings were grown in the light for 5 days, then incubated in darkness for 4 days, and treated with different concentrations of glucose (G) for 4 or 24 h. IN, input; IP immunoprecipitation; IB immunoblot. f Quantification of BAK1-GFP phosphorylation in Arabidopsis seedlings treated with different concentrations of glucose for 4 h from e . The ratio value of phosphorylated BAK1-GFP to immunoprecipitated BAK1-GFP was set at 1. Values for glucose treatments are given as mean ± SD ( n = 3). ** P < 0.01 compared with the value for 0% glucose treatment by Student’s t -test
Anti Bri1, supplied by Abiocode Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti bri1/product/Abiocode Inc
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
anti bri1 - by Bioz Stars, 2024-04
86/100 stars

Images

1) Product Images from "BRI1 and BAK1 interact with G proteins and regulate sugar-responsive growth and development in Arabidopsis"

Article Title: BRI1 and BAK1 interact with G proteins and regulate sugar-responsive growth and development in Arabidopsis

Journal: Nature Communications

doi: 10.1038/s41467-018-03884-8

Glucose influences the interactions between BRI1 and BAK1. a Glucose regulates the physical interactions between BRI1 and BAK1. pBRI1:BRI1-GFP ; 35S:BAK1 - HA or 35S:BAK1-HA seedlings were grown in the light for 5 days, then incubated in darkness for 4 days, and treated with different concentrations of glucose (G) for 4 or 24 h. IN input; IP immunoprecipitation; IB immunoblot. b Quantification of BRI1-GFP and BAK1-HA associations in Arabidopsis seedlings treated with different concentrations of glucose (G) for 4 h from a . The ratio value of immunoprecipitated BAK1-HA to input BAK1-HA in response to 0% glucose was set at 1. Values for glucose treatments are given as mean ± SD ( n = 3). ** P < 0.01 compared with the value for 0% glucose treatment by Student’s t -test. c Glucose influences the phosphorylation levels of BRI1 in Arabidopsis. pBRI1:BRI1-GFP seedlings were grown in the light for 5 days, then incubated in darkness for 4 days, and treated with different concentrations of glucose (G) for 4 or 24 h. IN input; IP immunoprecipitation; IB immunoblot. d Quantification of BRI1-GFP phosphorylation in Arabidopsis seedlings treated with different concentrations of glucose for 4 h from c . The ratio value of phosphorylated BRI1-GFP to immunoprecipitated BRI1-GFP in response to 0% glucose was set at 1. Values for glucose treatments are given as mean ± SD ( n = 3). ** P < 0.01 compared with the value for 0% glucose treatment by Student’s t -test. e Glucose influences the phosphorylation levels of BAK1 in Arabidopsis. pBAK1:BAK1-GFP seedlings were grown in the light for 5 days, then incubated in darkness for 4 days, and treated with different concentrations of glucose (G) for 4 or 24 h. IN, input; IP immunoprecipitation; IB immunoblot. f Quantification of BAK1-GFP phosphorylation in Arabidopsis seedlings treated with different concentrations of glucose for 4 h from e . The ratio value of phosphorylated BAK1-GFP to immunoprecipitated BAK1-GFP was set at 1. Values for glucose treatments are given as mean ± SD ( n = 3). ** P < 0.01 compared with the value for 0% glucose treatment by Student’s t -test
Figure Legend Snippet: Glucose influences the interactions between BRI1 and BAK1. a Glucose regulates the physical interactions between BRI1 and BAK1. pBRI1:BRI1-GFP ; 35S:BAK1 - HA or 35S:BAK1-HA seedlings were grown in the light for 5 days, then incubated in darkness for 4 days, and treated with different concentrations of glucose (G) for 4 or 24 h. IN input; IP immunoprecipitation; IB immunoblot. b Quantification of BRI1-GFP and BAK1-HA associations in Arabidopsis seedlings treated with different concentrations of glucose (G) for 4 h from a . The ratio value of immunoprecipitated BAK1-HA to input BAK1-HA in response to 0% glucose was set at 1. Values for glucose treatments are given as mean ± SD ( n = 3). ** P < 0.01 compared with the value for 0% glucose treatment by Student’s t -test. c Glucose influences the phosphorylation levels of BRI1 in Arabidopsis. pBRI1:BRI1-GFP seedlings were grown in the light for 5 days, then incubated in darkness for 4 days, and treated with different concentrations of glucose (G) for 4 or 24 h. IN input; IP immunoprecipitation; IB immunoblot. d Quantification of BRI1-GFP phosphorylation in Arabidopsis seedlings treated with different concentrations of glucose for 4 h from c . The ratio value of phosphorylated BRI1-GFP to immunoprecipitated BRI1-GFP in response to 0% glucose was set at 1. Values for glucose treatments are given as mean ± SD ( n = 3). ** P < 0.01 compared with the value for 0% glucose treatment by Student’s t -test. e Glucose influences the phosphorylation levels of BAK1 in Arabidopsis. pBAK1:BAK1-GFP seedlings were grown in the light for 5 days, then incubated in darkness for 4 days, and treated with different concentrations of glucose (G) for 4 or 24 h. IN, input; IP immunoprecipitation; IB immunoblot. f Quantification of BAK1-GFP phosphorylation in Arabidopsis seedlings treated with different concentrations of glucose for 4 h from e . The ratio value of phosphorylated BAK1-GFP to immunoprecipitated BAK1-GFP was set at 1. Values for glucose treatments are given as mean ± SD ( n = 3). ** P < 0.01 compared with the value for 0% glucose treatment by Student’s t -test

Techniques Used: Incubation, Immunoprecipitation, Western Blot

G-proteins act genetically with BRI1 and BAK1. a Comparison of development stages of Col-0 (1), bri1-301 (2), bak1-4 (3), agb1-2 (4), agb1-2 bri1-301 (5), agb1-2 bak1-4 (6), gpa1-101 (7), gpa1-101 bri1-301 (8), gpa1-101 bak1-4 (9), agg3-3 (10), agg3-3 bri1-301 , (11), and agg3-3 bak1-4 (12). Seedlings were grown vertically on medium supplemented with 1% glucose in the dark for 19 days ( n ≥ 85). b The percentage of the indicated seedlings with green cotyledons. Seedlings were grown on medium supplemented with 6% glucose under constant light condition for 16 days ( n ≥ 72). Values in a.b are given as mean ± SD
Figure Legend Snippet: G-proteins act genetically with BRI1 and BAK1. a Comparison of development stages of Col-0 (1), bri1-301 (2), bak1-4 (3), agb1-2 (4), agb1-2 bri1-301 (5), agb1-2 bak1-4 (6), gpa1-101 (7), gpa1-101 bri1-301 (8), gpa1-101 bak1-4 (9), agg3-3 (10), agg3-3 bri1-301 , (11), and agg3-3 bak1-4 (12). Seedlings were grown vertically on medium supplemented with 1% glucose in the dark for 19 days ( n ≥ 85). b The percentage of the indicated seedlings with green cotyledons. Seedlings were grown on medium supplemented with 6% glucose under constant light condition for 16 days ( n ≥ 72). Values in a.b are given as mean ± SD

Techniques Used:

BRI1 and BAK1 physically interact with G proteins. a BRI1 kinase domain (BRI1-KD) interacts with AGB1 and AGG3 in vitro. MBP-GPA1, MBP-AGB1, or MBP-AGG3 was pulled down by GST-BRI1-KD and detected by immunoblot with anti-MBP antibody. Arrow indicates the band of MBP-GPA1.IN, input; IB, immunoblot. b BAK1 kinase domain (BAK1-KD) interacts with AGG3 in vitro. MBP-GPA1, MBP-AGB1, or MBP-AGG3 was pulled down by GST-BAK1-KD and detected by immunoblot with anti-MBP antibody. Arrow indicates the band of MBP-GPA1. IN, input; IB immunoblot. c Bimolecular fluorescence complementation (BiFC) assays show that BRI1 interacts with AGB1 and AGG3 in N . benthamiana leaves. BRI1-nYFP was coexpressed with GPA1-cYFP, AGB1-cYFP, or AGG3-cYFP in leaves of N . benthamiana . d BiFC assays show that BAK1 interacts with AGG3 in N . benthamiana leaves. BAK1-nYFP was coexpressed with GPA1-cYFP, AGB1-cYFP, or AGG3-cYFP in leaves of N . benthamiana . e BRI1 interacts with AGB1 in Arabidopsis. The extracted total proteins from 35S:GFP ; 35S:Myc-AGB1 , or pBRI1:BRI1-GFP ; 35S:Myc-AGB1 seedlings were incubated with GFP-Trap-A, respectively. Immunoprecipitates were examined using anti-Myc and anti-GFP antibodies, respectively. IP immunoprecipitation; IN input; IB immunoblot. f BRI1 interacts with AGG3 in Arabidopsis. The extracted total proteins from pBRI1:BRI1-GFP ; 35S:GFP or pBRI1:BRI1-GFP ; 35S:Myc-AGG3 seedlings were incubated with GFP-Trap-A, and examined using anti-Myc and anti-GFP antibodies, respectively. IP immunoprecipitation; IN input; IB immunoblot. g BAK1 interacts with AGG3 in Arabidopsis. The extracted total proteins from 35S:GFP ; 35S:BAK1-HA and 35S:GFP-AGG3 ; 35S:BAK1-HA seedlings were incubated with GFP-Trap-A, and examined using anti-HA and anti-GFP antibodies, respectively. IP immunoprecipitation; IN input; IB immunoblot. Scale bars, 50 µm in c , d
Figure Legend Snippet: BRI1 and BAK1 physically interact with G proteins. a BRI1 kinase domain (BRI1-KD) interacts with AGB1 and AGG3 in vitro. MBP-GPA1, MBP-AGB1, or MBP-AGG3 was pulled down by GST-BRI1-KD and detected by immunoblot with anti-MBP antibody. Arrow indicates the band of MBP-GPA1.IN, input; IB, immunoblot. b BAK1 kinase domain (BAK1-KD) interacts with AGG3 in vitro. MBP-GPA1, MBP-AGB1, or MBP-AGG3 was pulled down by GST-BAK1-KD and detected by immunoblot with anti-MBP antibody. Arrow indicates the band of MBP-GPA1. IN, input; IB immunoblot. c Bimolecular fluorescence complementation (BiFC) assays show that BRI1 interacts with AGB1 and AGG3 in N . benthamiana leaves. BRI1-nYFP was coexpressed with GPA1-cYFP, AGB1-cYFP, or AGG3-cYFP in leaves of N . benthamiana . d BiFC assays show that BAK1 interacts with AGG3 in N . benthamiana leaves. BAK1-nYFP was coexpressed with GPA1-cYFP, AGB1-cYFP, or AGG3-cYFP in leaves of N . benthamiana . e BRI1 interacts with AGB1 in Arabidopsis. The extracted total proteins from 35S:GFP ; 35S:Myc-AGB1 , or pBRI1:BRI1-GFP ; 35S:Myc-AGB1 seedlings were incubated with GFP-Trap-A, respectively. Immunoprecipitates were examined using anti-Myc and anti-GFP antibodies, respectively. IP immunoprecipitation; IN input; IB immunoblot. f BRI1 interacts with AGG3 in Arabidopsis. The extracted total proteins from pBRI1:BRI1-GFP ; 35S:GFP or pBRI1:BRI1-GFP ; 35S:Myc-AGG3 seedlings were incubated with GFP-Trap-A, and examined using anti-Myc and anti-GFP antibodies, respectively. IP immunoprecipitation; IN input; IB immunoblot. g BAK1 interacts with AGG3 in Arabidopsis. The extracted total proteins from 35S:GFP ; 35S:BAK1-HA and 35S:GFP-AGG3 ; 35S:BAK1-HA seedlings were incubated with GFP-Trap-A, and examined using anti-HA and anti-GFP antibodies, respectively. IP immunoprecipitation; IN input; IB immunoblot. Scale bars, 50 µm in c , d

Techniques Used: In Vitro, Western Blot, Fluorescence, Incubation, Immunoprecipitation

BRI1 is required for the phosphorylation of AGB1 and AGG3. a BRI1 kinase domain (BRI1-KD) phosphorylates AGB1 in vitro, but bri1-301 kinase domain (bri1-301-KD) does not. The phosphorylated MBP-AGB1 (pMBP-AGB1), unphosphorylated (MBP-AGB1) and autophosphorylated GST-BRI1-KD (pGST-BRI1-KD) were separated by phos-tag SDS-PAGE. IN, input. b BRI1-KD phosphorylates AGG3 in vitro, but bri1-301-KD does not. The phosphorylated MBP-AGG3 (pMBP-AGG3), unphosphorylated (MBP-AGG3) and autophosphorylated GST-BRI1-KD (pGST-BRI1-KD) were separated by phos-tag SDS-PAGE. IN, input. c , d BRI1 is required for the phosphorylation of AGB1 in Arabidopsis . Total proteins from pBRI1:BRI1-GFP ; 35S:Myc-AGB1 (L1) and 35S:GFP ; 35S:Myc-AGB1 (L2) seedlings were mixed with anti-Myc-Tag mouse mAb conjugated agarose beads, and detected using anti-Myc and anti-phosphothreonine (pThr) antibodies, respectively c . Quantification of the phosphorylated Myc-AGB1 was relative to the immunoprecipitated Myc-AGB1 d . Values are given as mean ± SD ( n = 3). ** P < 0.01 compared with the value for L2 seedlings by Student’s t -test. e , f BRI1 is required for the phosphorylation of AGG3 in Arabidopsis . Total proteins from pBRI1:BRI1-GFP ; 35S:Myc-AGG3 (L3) and 35S:GFP ; 35S:Myc-AGG3 (L4) seedlings were mixed with anti-Myc-Tag mouse mAb conjugated agarose beads and detected using anti-Myc and anti-phosphothreonine (pThr) antibodies, respectively ( e ). Quantification of the phosphorylated Myc-AGG3 was relative to the immunoprecipitated Myc-AGG3 f . Values are given as mean ± SD ( n = 3).** P < 0.01 compared with the value for L4 seedlings by Student’s t -test. g , h BRI1 is required for the phosphorylation of AGB1 in Arabidopsis . Total proteins from 35S:GFP-AGB1 (L5) and 35S:GFP-AGB1 ; bri1-301 (L6) seedlings were incubated with GFP-Trap-A, and detected using anti-GFP and anti-phosphothreonine (pThr) antibodies, respectively g . Quantification of the phosphorylated GFP-AGB1 was relative to the immunoprecipitated GFP-AGB1 h . Values are given as mean ± SD, ( n = 3).** P < 0.01 compared with the value for L5 seedlings by Student’s t -test. i , j BRI1 is required for the phosphorylation of AGG3 in Arabidopsis . Total proteins from 35S:GFP-AGG3 (L7) and 35S:GFP-AGG3 ; bri1-301 (L8) seedlings were incubated with GFP beads, and detected by immunoblot with anti-GFP and anti-phosphothreonine (pThr) antibodies, respectively i . Quantification of the phosphorylated GFP-AGG3 was relative to the immunoprecipitated GFP-AGG3 j . Values are given as mean ± SD ( n = 3).** P < 0.01 compared with the value for L7 seedlings by Student’s t -test
Figure Legend Snippet: BRI1 is required for the phosphorylation of AGB1 and AGG3. a BRI1 kinase domain (BRI1-KD) phosphorylates AGB1 in vitro, but bri1-301 kinase domain (bri1-301-KD) does not. The phosphorylated MBP-AGB1 (pMBP-AGB1), unphosphorylated (MBP-AGB1) and autophosphorylated GST-BRI1-KD (pGST-BRI1-KD) were separated by phos-tag SDS-PAGE. IN, input. b BRI1-KD phosphorylates AGG3 in vitro, but bri1-301-KD does not. The phosphorylated MBP-AGG3 (pMBP-AGG3), unphosphorylated (MBP-AGG3) and autophosphorylated GST-BRI1-KD (pGST-BRI1-KD) were separated by phos-tag SDS-PAGE. IN, input. c , d BRI1 is required for the phosphorylation of AGB1 in Arabidopsis . Total proteins from pBRI1:BRI1-GFP ; 35S:Myc-AGB1 (L1) and 35S:GFP ; 35S:Myc-AGB1 (L2) seedlings were mixed with anti-Myc-Tag mouse mAb conjugated agarose beads, and detected using anti-Myc and anti-phosphothreonine (pThr) antibodies, respectively c . Quantification of the phosphorylated Myc-AGB1 was relative to the immunoprecipitated Myc-AGB1 d . Values are given as mean ± SD ( n = 3). ** P < 0.01 compared with the value for L2 seedlings by Student’s t -test. e , f BRI1 is required for the phosphorylation of AGG3 in Arabidopsis . Total proteins from pBRI1:BRI1-GFP ; 35S:Myc-AGG3 (L3) and 35S:GFP ; 35S:Myc-AGG3 (L4) seedlings were mixed with anti-Myc-Tag mouse mAb conjugated agarose beads and detected using anti-Myc and anti-phosphothreonine (pThr) antibodies, respectively ( e ). Quantification of the phosphorylated Myc-AGG3 was relative to the immunoprecipitated Myc-AGG3 f . Values are given as mean ± SD ( n = 3).** P < 0.01 compared with the value for L4 seedlings by Student’s t -test. g , h BRI1 is required for the phosphorylation of AGB1 in Arabidopsis . Total proteins from 35S:GFP-AGB1 (L5) and 35S:GFP-AGB1 ; bri1-301 (L6) seedlings were incubated with GFP-Trap-A, and detected using anti-GFP and anti-phosphothreonine (pThr) antibodies, respectively g . Quantification of the phosphorylated GFP-AGB1 was relative to the immunoprecipitated GFP-AGB1 h . Values are given as mean ± SD, ( n = 3).** P < 0.01 compared with the value for L5 seedlings by Student’s t -test. i , j BRI1 is required for the phosphorylation of AGG3 in Arabidopsis . Total proteins from 35S:GFP-AGG3 (L7) and 35S:GFP-AGG3 ; bri1-301 (L8) seedlings were incubated with GFP beads, and detected by immunoblot with anti-GFP and anti-phosphothreonine (pThr) antibodies, respectively i . Quantification of the phosphorylated GFP-AGG3 was relative to the immunoprecipitated GFP-AGG3 j . Values are given as mean ± SD ( n = 3).** P < 0.01 compared with the value for L7 seedlings by Student’s t -test

Techniques Used: In Vitro, SDS Page, Immunoprecipitation, Incubation, Western Blot

BRI1 and BAK1 affect the interactions between GPA1 and AGB1. a BRI1 and BAK1 influences the interactions between GPA1 and AGB1 in Arabidopsis , respectively. The wild type, bri1-301 , and bak1-4 leaf protoplasts co-transformed with 35S:AGB1 - Myc and 35S : GPA1 - HA plasmids were grown in darkness for 14 h, and then treated without or with 2% glucose (G) for 5 h. Total proteins from these leaf protoplasts were incubated with anti-Myc-Tag mouse mAb conjugated agarose beads, and detected using anti-Myc and anti-HA antibodies, respectively. IN input; IP immunoprecipitation. b – d Quantification of relative interactions between GPA1 and AGB1 in wild type ( b ), bri1-301 ( c ) and bak1-4 ( d ) leaf protoplasts treated without or with 2% glucose as shown in a . The ratio was shown as immunoprecipitated GPA1-HA to input GPA1-HA. Values are given as mean ± SD ( n = 3) relative to the value for 0% glucose, set at 1. Values in b – d are given as mean ± SD, ** P < 0.01 compared with the value for 0% glucose treatment by Student’s t -test
Figure Legend Snippet: BRI1 and BAK1 affect the interactions between GPA1 and AGB1. a BRI1 and BAK1 influences the interactions between GPA1 and AGB1 in Arabidopsis , respectively. The wild type, bri1-301 , and bak1-4 leaf protoplasts co-transformed with 35S:AGB1 - Myc and 35S : GPA1 - HA plasmids were grown in darkness for 14 h, and then treated without or with 2% glucose (G) for 5 h. Total proteins from these leaf protoplasts were incubated with anti-Myc-Tag mouse mAb conjugated agarose beads, and detected using anti-Myc and anti-HA antibodies, respectively. IN input; IP immunoprecipitation. b – d Quantification of relative interactions between GPA1 and AGB1 in wild type ( b ), bri1-301 ( c ) and bak1-4 ( d ) leaf protoplasts treated without or with 2% glucose as shown in a . The ratio was shown as immunoprecipitated GPA1-HA to input GPA1-HA. Values are given as mean ± SD ( n = 3) relative to the value for 0% glucose, set at 1. Values in b – d are given as mean ± SD, ** P < 0.01 compared with the value for 0% glucose treatment by Student’s t -test

Techniques Used: Transformation Assay, Incubation, Immunoprecipitation

BRI1 and BAK1 play key roles in sugar responses. a Different stages of dark-grown wild-type seedlings used for scoring sugar responses. b Comparison of developmental stages between Col-0, bri1-301 , and bak1-4 . Seedlings were grown vertically on medium supplemented with 1% glucose (G) or 1% mannitol (M) in the dark for 19 days ( n ≥ 83). c The percentage of Col-0, bri1-301 , and bak1-4 seedlings with green cotyledons. Seedlings were grown on medium supplemented with 6% glucose (G) or 6% mannitol (M) under constant light condition for 10 days ( n ≥ 89). Values in b , c are given as mean ± SD. ** P < 0.01 compared with the wild type by Student’s t -test. Scale bars, 2 mm ( a )
Figure Legend Snippet: BRI1 and BAK1 play key roles in sugar responses. a Different stages of dark-grown wild-type seedlings used for scoring sugar responses. b Comparison of developmental stages between Col-0, bri1-301 , and bak1-4 . Seedlings were grown vertically on medium supplemented with 1% glucose (G) or 1% mannitol (M) in the dark for 19 days ( n ≥ 83). c The percentage of Col-0, bri1-301 , and bak1-4 seedlings with green cotyledons. Seedlings were grown on medium supplemented with 6% glucose (G) or 6% mannitol (M) under constant light condition for 10 days ( n ≥ 89). Values in b , c are given as mean ± SD. ** P < 0.01 compared with the wild type by Student’s t -test. Scale bars, 2 mm ( a )

Techniques Used:


Structured Review

Abiocode Inc anti bri1
Glucose influences the interactions between <t>BRI1</t> and BAK1. a Glucose regulates the physical interactions between BRI1 and BAK1. pBRI1:BRI1-GFP ; 35S:BAK1 - HA or 35S:BAK1-HA seedlings were grown in the light for 5 days, then incubated in darkness for 4 days, and treated with different concentrations of glucose (G) for 4 or 24 h. IN input; IP immunoprecipitation; IB immunoblot. b Quantification of BRI1-GFP and BAK1-HA associations in Arabidopsis seedlings treated with different concentrations of glucose (G) for 4 h from a . The ratio value of immunoprecipitated BAK1-HA to input BAK1-HA in response to 0% glucose was set at 1. Values for glucose treatments are given as mean ± SD ( n = 3). ** P < 0.01 compared with the value for 0% glucose treatment by Student’s t -test. c Glucose influences the phosphorylation levels of BRI1 in Arabidopsis. pBRI1:BRI1-GFP seedlings were grown in the light for 5 days, then incubated in darkness for 4 days, and treated with different concentrations of glucose (G) for 4 or 24 h. IN input; IP immunoprecipitation; IB immunoblot. d Quantification of BRI1-GFP phosphorylation in Arabidopsis seedlings treated with different concentrations of glucose for 4 h from c . The ratio value of phosphorylated BRI1-GFP to immunoprecipitated BRI1-GFP in response to 0% glucose was set at 1. Values for glucose treatments are given as mean ± SD ( n = 3). ** P < 0.01 compared with the value for 0% glucose treatment by Student’s t -test. e Glucose influences the phosphorylation levels of BAK1 in Arabidopsis. pBAK1:BAK1-GFP seedlings were grown in the light for 5 days, then incubated in darkness for 4 days, and treated with different concentrations of glucose (G) for 4 or 24 h. IN, input; IP immunoprecipitation; IB immunoblot. f Quantification of BAK1-GFP phosphorylation in Arabidopsis seedlings treated with different concentrations of glucose for 4 h from e . The ratio value of phosphorylated BAK1-GFP to immunoprecipitated BAK1-GFP was set at 1. Values for glucose treatments are given as mean ± SD ( n = 3). ** P < 0.01 compared with the value for 0% glucose treatment by Student’s t -test
Anti Bri1, supplied by Abiocode Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti bri1/product/Abiocode Inc
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
anti bri1 - by Bioz Stars, 2024-04
86/100 stars

Images

1) Product Images from "BRI1 and BAK1 interact with G proteins and regulate sugar-responsive growth and development in Arabidopsis"

Article Title: BRI1 and BAK1 interact with G proteins and regulate sugar-responsive growth and development in Arabidopsis

Journal: Nature Communications

doi: 10.1038/s41467-018-03884-8

Glucose influences the interactions between BRI1 and BAK1. a Glucose regulates the physical interactions between BRI1 and BAK1. pBRI1:BRI1-GFP ; 35S:BAK1 - HA or 35S:BAK1-HA seedlings were grown in the light for 5 days, then incubated in darkness for 4 days, and treated with different concentrations of glucose (G) for 4 or 24 h. IN input; IP immunoprecipitation; IB immunoblot. b Quantification of BRI1-GFP and BAK1-HA associations in Arabidopsis seedlings treated with different concentrations of glucose (G) for 4 h from a . The ratio value of immunoprecipitated BAK1-HA to input BAK1-HA in response to 0% glucose was set at 1. Values for glucose treatments are given as mean ± SD ( n = 3). ** P < 0.01 compared with the value for 0% glucose treatment by Student’s t -test. c Glucose influences the phosphorylation levels of BRI1 in Arabidopsis. pBRI1:BRI1-GFP seedlings were grown in the light for 5 days, then incubated in darkness for 4 days, and treated with different concentrations of glucose (G) for 4 or 24 h. IN input; IP immunoprecipitation; IB immunoblot. d Quantification of BRI1-GFP phosphorylation in Arabidopsis seedlings treated with different concentrations of glucose for 4 h from c . The ratio value of phosphorylated BRI1-GFP to immunoprecipitated BRI1-GFP in response to 0% glucose was set at 1. Values for glucose treatments are given as mean ± SD ( n = 3). ** P < 0.01 compared with the value for 0% glucose treatment by Student’s t -test. e Glucose influences the phosphorylation levels of BAK1 in Arabidopsis. pBAK1:BAK1-GFP seedlings were grown in the light for 5 days, then incubated in darkness for 4 days, and treated with different concentrations of glucose (G) for 4 or 24 h. IN, input; IP immunoprecipitation; IB immunoblot. f Quantification of BAK1-GFP phosphorylation in Arabidopsis seedlings treated with different concentrations of glucose for 4 h from e . The ratio value of phosphorylated BAK1-GFP to immunoprecipitated BAK1-GFP was set at 1. Values for glucose treatments are given as mean ± SD ( n = 3). ** P < 0.01 compared with the value for 0% glucose treatment by Student’s t -test
Figure Legend Snippet: Glucose influences the interactions between BRI1 and BAK1. a Glucose regulates the physical interactions between BRI1 and BAK1. pBRI1:BRI1-GFP ; 35S:BAK1 - HA or 35S:BAK1-HA seedlings were grown in the light for 5 days, then incubated in darkness for 4 days, and treated with different concentrations of glucose (G) for 4 or 24 h. IN input; IP immunoprecipitation; IB immunoblot. b Quantification of BRI1-GFP and BAK1-HA associations in Arabidopsis seedlings treated with different concentrations of glucose (G) for 4 h from a . The ratio value of immunoprecipitated BAK1-HA to input BAK1-HA in response to 0% glucose was set at 1. Values for glucose treatments are given as mean ± SD ( n = 3). ** P < 0.01 compared with the value for 0% glucose treatment by Student’s t -test. c Glucose influences the phosphorylation levels of BRI1 in Arabidopsis. pBRI1:BRI1-GFP seedlings were grown in the light for 5 days, then incubated in darkness for 4 days, and treated with different concentrations of glucose (G) for 4 or 24 h. IN input; IP immunoprecipitation; IB immunoblot. d Quantification of BRI1-GFP phosphorylation in Arabidopsis seedlings treated with different concentrations of glucose for 4 h from c . The ratio value of phosphorylated BRI1-GFP to immunoprecipitated BRI1-GFP in response to 0% glucose was set at 1. Values for glucose treatments are given as mean ± SD ( n = 3). ** P < 0.01 compared with the value for 0% glucose treatment by Student’s t -test. e Glucose influences the phosphorylation levels of BAK1 in Arabidopsis. pBAK1:BAK1-GFP seedlings were grown in the light for 5 days, then incubated in darkness for 4 days, and treated with different concentrations of glucose (G) for 4 or 24 h. IN, input; IP immunoprecipitation; IB immunoblot. f Quantification of BAK1-GFP phosphorylation in Arabidopsis seedlings treated with different concentrations of glucose for 4 h from e . The ratio value of phosphorylated BAK1-GFP to immunoprecipitated BAK1-GFP was set at 1. Values for glucose treatments are given as mean ± SD ( n = 3). ** P < 0.01 compared with the value for 0% glucose treatment by Student’s t -test

Techniques Used: Incubation, Immunoprecipitation, Western Blot

G-proteins act genetically with BRI1 and BAK1. a Comparison of development stages of Col-0 (1), bri1-301 (2), bak1-4 (3), agb1-2 (4), agb1-2 bri1-301 (5), agb1-2 bak1-4 (6), gpa1-101 (7), gpa1-101 bri1-301 (8), gpa1-101 bak1-4 (9), agg3-3 (10), agg3-3 bri1-301 , (11), and agg3-3 bak1-4 (12). Seedlings were grown vertically on medium supplemented with 1% glucose in the dark for 19 days ( n ≥ 85). b The percentage of the indicated seedlings with green cotyledons. Seedlings were grown on medium supplemented with 6% glucose under constant light condition for 16 days ( n ≥ 72). Values in a.b are given as mean ± SD
Figure Legend Snippet: G-proteins act genetically with BRI1 and BAK1. a Comparison of development stages of Col-0 (1), bri1-301 (2), bak1-4 (3), agb1-2 (4), agb1-2 bri1-301 (5), agb1-2 bak1-4 (6), gpa1-101 (7), gpa1-101 bri1-301 (8), gpa1-101 bak1-4 (9), agg3-3 (10), agg3-3 bri1-301 , (11), and agg3-3 bak1-4 (12). Seedlings were grown vertically on medium supplemented with 1% glucose in the dark for 19 days ( n ≥ 85). b The percentage of the indicated seedlings with green cotyledons. Seedlings were grown on medium supplemented with 6% glucose under constant light condition for 16 days ( n ≥ 72). Values in a.b are given as mean ± SD

Techniques Used:

BRI1 and BAK1 physically interact with G proteins. a BRI1 kinase domain (BRI1-KD) interacts with AGB1 and AGG3 in vitro. MBP-GPA1, MBP-AGB1, or MBP-AGG3 was pulled down by GST-BRI1-KD and detected by immunoblot with anti-MBP antibody. Arrow indicates the band of MBP-GPA1.IN, input; IB, immunoblot. b BAK1 kinase domain (BAK1-KD) interacts with AGG3 in vitro. MBP-GPA1, MBP-AGB1, or MBP-AGG3 was pulled down by GST-BAK1-KD and detected by immunoblot with anti-MBP antibody. Arrow indicates the band of MBP-GPA1. IN, input; IB immunoblot. c Bimolecular fluorescence complementation (BiFC) assays show that BRI1 interacts with AGB1 and AGG3 in N . benthamiana leaves. BRI1-nYFP was coexpressed with GPA1-cYFP, AGB1-cYFP, or AGG3-cYFP in leaves of N . benthamiana . d BiFC assays show that BAK1 interacts with AGG3 in N . benthamiana leaves. BAK1-nYFP was coexpressed with GPA1-cYFP, AGB1-cYFP, or AGG3-cYFP in leaves of N . benthamiana . e BRI1 interacts with AGB1 in Arabidopsis. The extracted total proteins from 35S:GFP ; 35S:Myc-AGB1 , or pBRI1:BRI1-GFP ; 35S:Myc-AGB1 seedlings were incubated with GFP-Trap-A, respectively. Immunoprecipitates were examined using anti-Myc and anti-GFP antibodies, respectively. IP immunoprecipitation; IN input; IB immunoblot. f BRI1 interacts with AGG3 in Arabidopsis. The extracted total proteins from pBRI1:BRI1-GFP ; 35S:GFP or pBRI1:BRI1-GFP ; 35S:Myc-AGG3 seedlings were incubated with GFP-Trap-A, and examined using anti-Myc and anti-GFP antibodies, respectively. IP immunoprecipitation; IN input; IB immunoblot. g BAK1 interacts with AGG3 in Arabidopsis. The extracted total proteins from 35S:GFP ; 35S:BAK1-HA and 35S:GFP-AGG3 ; 35S:BAK1-HA seedlings were incubated with GFP-Trap-A, and examined using anti-HA and anti-GFP antibodies, respectively. IP immunoprecipitation; IN input; IB immunoblot. Scale bars, 50 µm in c , d
Figure Legend Snippet: BRI1 and BAK1 physically interact with G proteins. a BRI1 kinase domain (BRI1-KD) interacts with AGB1 and AGG3 in vitro. MBP-GPA1, MBP-AGB1, or MBP-AGG3 was pulled down by GST-BRI1-KD and detected by immunoblot with anti-MBP antibody. Arrow indicates the band of MBP-GPA1.IN, input; IB, immunoblot. b BAK1 kinase domain (BAK1-KD) interacts with AGG3 in vitro. MBP-GPA1, MBP-AGB1, or MBP-AGG3 was pulled down by GST-BAK1-KD and detected by immunoblot with anti-MBP antibody. Arrow indicates the band of MBP-GPA1. IN, input; IB immunoblot. c Bimolecular fluorescence complementation (BiFC) assays show that BRI1 interacts with AGB1 and AGG3 in N . benthamiana leaves. BRI1-nYFP was coexpressed with GPA1-cYFP, AGB1-cYFP, or AGG3-cYFP in leaves of N . benthamiana . d BiFC assays show that BAK1 interacts with AGG3 in N . benthamiana leaves. BAK1-nYFP was coexpressed with GPA1-cYFP, AGB1-cYFP, or AGG3-cYFP in leaves of N . benthamiana . e BRI1 interacts with AGB1 in Arabidopsis. The extracted total proteins from 35S:GFP ; 35S:Myc-AGB1 , or pBRI1:BRI1-GFP ; 35S:Myc-AGB1 seedlings were incubated with GFP-Trap-A, respectively. Immunoprecipitates were examined using anti-Myc and anti-GFP antibodies, respectively. IP immunoprecipitation; IN input; IB immunoblot. f BRI1 interacts with AGG3 in Arabidopsis. The extracted total proteins from pBRI1:BRI1-GFP ; 35S:GFP or pBRI1:BRI1-GFP ; 35S:Myc-AGG3 seedlings were incubated with GFP-Trap-A, and examined using anti-Myc and anti-GFP antibodies, respectively. IP immunoprecipitation; IN input; IB immunoblot. g BAK1 interacts with AGG3 in Arabidopsis. The extracted total proteins from 35S:GFP ; 35S:BAK1-HA and 35S:GFP-AGG3 ; 35S:BAK1-HA seedlings were incubated with GFP-Trap-A, and examined using anti-HA and anti-GFP antibodies, respectively. IP immunoprecipitation; IN input; IB immunoblot. Scale bars, 50 µm in c , d

Techniques Used: In Vitro, Western Blot, Fluorescence, Incubation, Immunoprecipitation

BRI1 is required for the phosphorylation of AGB1 and AGG3. a BRI1 kinase domain (BRI1-KD) phosphorylates AGB1 in vitro, but bri1-301 kinase domain (bri1-301-KD) does not. The phosphorylated MBP-AGB1 (pMBP-AGB1), unphosphorylated (MBP-AGB1) and autophosphorylated GST-BRI1-KD (pGST-BRI1-KD) were separated by phos-tag SDS-PAGE. IN, input. b BRI1-KD phosphorylates AGG3 in vitro, but bri1-301-KD does not. The phosphorylated MBP-AGG3 (pMBP-AGG3), unphosphorylated (MBP-AGG3) and autophosphorylated GST-BRI1-KD (pGST-BRI1-KD) were separated by phos-tag SDS-PAGE. IN, input. c , d BRI1 is required for the phosphorylation of AGB1 in Arabidopsis . Total proteins from pBRI1:BRI1-GFP ; 35S:Myc-AGB1 (L1) and 35S:GFP ; 35S:Myc-AGB1 (L2) seedlings were mixed with anti-Myc-Tag mouse mAb conjugated agarose beads, and detected using anti-Myc and anti-phosphothreonine (pThr) antibodies, respectively c . Quantification of the phosphorylated Myc-AGB1 was relative to the immunoprecipitated Myc-AGB1 d . Values are given as mean ± SD ( n = 3). ** P < 0.01 compared with the value for L2 seedlings by Student’s t -test. e , f BRI1 is required for the phosphorylation of AGG3 in Arabidopsis . Total proteins from pBRI1:BRI1-GFP ; 35S:Myc-AGG3 (L3) and 35S:GFP ; 35S:Myc-AGG3 (L4) seedlings were mixed with anti-Myc-Tag mouse mAb conjugated agarose beads and detected using anti-Myc and anti-phosphothreonine (pThr) antibodies, respectively ( e ). Quantification of the phosphorylated Myc-AGG3 was relative to the immunoprecipitated Myc-AGG3 f . Values are given as mean ± SD ( n = 3).** P < 0.01 compared with the value for L4 seedlings by Student’s t -test. g , h BRI1 is required for the phosphorylation of AGB1 in Arabidopsis . Total proteins from 35S:GFP-AGB1 (L5) and 35S:GFP-AGB1 ; bri1-301 (L6) seedlings were incubated with GFP-Trap-A, and detected using anti-GFP and anti-phosphothreonine (pThr) antibodies, respectively g . Quantification of the phosphorylated GFP-AGB1 was relative to the immunoprecipitated GFP-AGB1 h . Values are given as mean ± SD, ( n = 3).** P < 0.01 compared with the value for L5 seedlings by Student’s t -test. i , j BRI1 is required for the phosphorylation of AGG3 in Arabidopsis . Total proteins from 35S:GFP-AGG3 (L7) and 35S:GFP-AGG3 ; bri1-301 (L8) seedlings were incubated with GFP beads, and detected by immunoblot with anti-GFP and anti-phosphothreonine (pThr) antibodies, respectively i . Quantification of the phosphorylated GFP-AGG3 was relative to the immunoprecipitated GFP-AGG3 j . Values are given as mean ± SD ( n = 3).** P < 0.01 compared with the value for L7 seedlings by Student’s t -test
Figure Legend Snippet: BRI1 is required for the phosphorylation of AGB1 and AGG3. a BRI1 kinase domain (BRI1-KD) phosphorylates AGB1 in vitro, but bri1-301 kinase domain (bri1-301-KD) does not. The phosphorylated MBP-AGB1 (pMBP-AGB1), unphosphorylated (MBP-AGB1) and autophosphorylated GST-BRI1-KD (pGST-BRI1-KD) were separated by phos-tag SDS-PAGE. IN, input. b BRI1-KD phosphorylates AGG3 in vitro, but bri1-301-KD does not. The phosphorylated MBP-AGG3 (pMBP-AGG3), unphosphorylated (MBP-AGG3) and autophosphorylated GST-BRI1-KD (pGST-BRI1-KD) were separated by phos-tag SDS-PAGE. IN, input. c , d BRI1 is required for the phosphorylation of AGB1 in Arabidopsis . Total proteins from pBRI1:BRI1-GFP ; 35S:Myc-AGB1 (L1) and 35S:GFP ; 35S:Myc-AGB1 (L2) seedlings were mixed with anti-Myc-Tag mouse mAb conjugated agarose beads, and detected using anti-Myc and anti-phosphothreonine (pThr) antibodies, respectively c . Quantification of the phosphorylated Myc-AGB1 was relative to the immunoprecipitated Myc-AGB1 d . Values are given as mean ± SD ( n = 3). ** P < 0.01 compared with the value for L2 seedlings by Student’s t -test. e , f BRI1 is required for the phosphorylation of AGG3 in Arabidopsis . Total proteins from pBRI1:BRI1-GFP ; 35S:Myc-AGG3 (L3) and 35S:GFP ; 35S:Myc-AGG3 (L4) seedlings were mixed with anti-Myc-Tag mouse mAb conjugated agarose beads and detected using anti-Myc and anti-phosphothreonine (pThr) antibodies, respectively ( e ). Quantification of the phosphorylated Myc-AGG3 was relative to the immunoprecipitated Myc-AGG3 f . Values are given as mean ± SD ( n = 3).** P < 0.01 compared with the value for L4 seedlings by Student’s t -test. g , h BRI1 is required for the phosphorylation of AGB1 in Arabidopsis . Total proteins from 35S:GFP-AGB1 (L5) and 35S:GFP-AGB1 ; bri1-301 (L6) seedlings were incubated with GFP-Trap-A, and detected using anti-GFP and anti-phosphothreonine (pThr) antibodies, respectively g . Quantification of the phosphorylated GFP-AGB1 was relative to the immunoprecipitated GFP-AGB1 h . Values are given as mean ± SD, ( n = 3).** P < 0.01 compared with the value for L5 seedlings by Student’s t -test. i , j BRI1 is required for the phosphorylation of AGG3 in Arabidopsis . Total proteins from 35S:GFP-AGG3 (L7) and 35S:GFP-AGG3 ; bri1-301 (L8) seedlings were incubated with GFP beads, and detected by immunoblot with anti-GFP and anti-phosphothreonine (pThr) antibodies, respectively i . Quantification of the phosphorylated GFP-AGG3 was relative to the immunoprecipitated GFP-AGG3 j . Values are given as mean ± SD ( n = 3).** P < 0.01 compared with the value for L7 seedlings by Student’s t -test

Techniques Used: In Vitro, SDS Page, Immunoprecipitation, Incubation, Western Blot

BRI1 and BAK1 affect the interactions between GPA1 and AGB1. a BRI1 and BAK1 influences the interactions between GPA1 and AGB1 in Arabidopsis , respectively. The wild type, bri1-301 , and bak1-4 leaf protoplasts co-transformed with 35S:AGB1 - Myc and 35S : GPA1 - HA plasmids were grown in darkness for 14 h, and then treated without or with 2% glucose (G) for 5 h. Total proteins from these leaf protoplasts were incubated with anti-Myc-Tag mouse mAb conjugated agarose beads, and detected using anti-Myc and anti-HA antibodies, respectively. IN input; IP immunoprecipitation. b – d Quantification of relative interactions between GPA1 and AGB1 in wild type ( b ), bri1-301 ( c ) and bak1-4 ( d ) leaf protoplasts treated without or with 2% glucose as shown in a . The ratio was shown as immunoprecipitated GPA1-HA to input GPA1-HA. Values are given as mean ± SD ( n = 3) relative to the value for 0% glucose, set at 1. Values in b – d are given as mean ± SD, ** P < 0.01 compared with the value for 0% glucose treatment by Student’s t -test
Figure Legend Snippet: BRI1 and BAK1 affect the interactions between GPA1 and AGB1. a BRI1 and BAK1 influences the interactions between GPA1 and AGB1 in Arabidopsis , respectively. The wild type, bri1-301 , and bak1-4 leaf protoplasts co-transformed with 35S:AGB1 - Myc and 35S : GPA1 - HA plasmids were grown in darkness for 14 h, and then treated without or with 2% glucose (G) for 5 h. Total proteins from these leaf protoplasts were incubated with anti-Myc-Tag mouse mAb conjugated agarose beads, and detected using anti-Myc and anti-HA antibodies, respectively. IN input; IP immunoprecipitation. b – d Quantification of relative interactions between GPA1 and AGB1 in wild type ( b ), bri1-301 ( c ) and bak1-4 ( d ) leaf protoplasts treated without or with 2% glucose as shown in a . The ratio was shown as immunoprecipitated GPA1-HA to input GPA1-HA. Values are given as mean ± SD ( n = 3) relative to the value for 0% glucose, set at 1. Values in b – d are given as mean ± SD, ** P < 0.01 compared with the value for 0% glucose treatment by Student’s t -test

Techniques Used: Transformation Assay, Incubation, Immunoprecipitation

BRI1 and BAK1 play key roles in sugar responses. a Different stages of dark-grown wild-type seedlings used for scoring sugar responses. b Comparison of developmental stages between Col-0, bri1-301 , and bak1-4 . Seedlings were grown vertically on medium supplemented with 1% glucose (G) or 1% mannitol (M) in the dark for 19 days ( n ≥ 83). c The percentage of Col-0, bri1-301 , and bak1-4 seedlings with green cotyledons. Seedlings were grown on medium supplemented with 6% glucose (G) or 6% mannitol (M) under constant light condition for 10 days ( n ≥ 89). Values in b , c are given as mean ± SD. ** P < 0.01 compared with the wild type by Student’s t -test. Scale bars, 2 mm ( a )
Figure Legend Snippet: BRI1 and BAK1 play key roles in sugar responses. a Different stages of dark-grown wild-type seedlings used for scoring sugar responses. b Comparison of developmental stages between Col-0, bri1-301 , and bak1-4 . Seedlings were grown vertically on medium supplemented with 1% glucose (G) or 1% mannitol (M) in the dark for 19 days ( n ≥ 83). c The percentage of Col-0, bri1-301 , and bak1-4 seedlings with green cotyledons. Seedlings were grown on medium supplemented with 6% glucose (G) or 6% mannitol (M) under constant light condition for 10 days ( n ≥ 89). Values in b , c are given as mean ± SD. ** P < 0.01 compared with the wild type by Student’s t -test. Scale bars, 2 mm ( a )

Techniques Used:


Structured Review

Abiocode Inc anti bri1
Glucose influences the interactions between <t>BRI1</t> and BAK1. a Glucose regulates the physical interactions between BRI1 and BAK1. pBRI1:BRI1-GFP ; 35S:BAK1 - HA or 35S:BAK1-HA seedlings were grown in the light for 5 days, then incubated in darkness for 4 days, and treated with different concentrations of glucose (G) for 4 or 24 h. IN input; IP immunoprecipitation; IB immunoblot. b Quantification of BRI1-GFP and BAK1-HA associations in Arabidopsis seedlings treated with different concentrations of glucose (G) for 4 h from a . The ratio value of immunoprecipitated BAK1-HA to input BAK1-HA in response to 0% glucose was set at 1. Values for glucose treatments are given as mean ± SD ( n = 3). ** P < 0.01 compared with the value for 0% glucose treatment by Student’s t -test. c Glucose influences the phosphorylation levels of BRI1 in Arabidopsis. pBRI1:BRI1-GFP seedlings were grown in the light for 5 days, then incubated in darkness for 4 days, and treated with different concentrations of glucose (G) for 4 or 24 h. IN input; IP immunoprecipitation; IB immunoblot. d Quantification of BRI1-GFP phosphorylation in Arabidopsis seedlings treated with different concentrations of glucose for 4 h from c . The ratio value of phosphorylated BRI1-GFP to immunoprecipitated BRI1-GFP in response to 0% glucose was set at 1. Values for glucose treatments are given as mean ± SD ( n = 3). ** P < 0.01 compared with the value for 0% glucose treatment by Student’s t -test. e Glucose influences the phosphorylation levels of BAK1 in Arabidopsis. pBAK1:BAK1-GFP seedlings were grown in the light for 5 days, then incubated in darkness for 4 days, and treated with different concentrations of glucose (G) for 4 or 24 h. IN, input; IP immunoprecipitation; IB immunoblot. f Quantification of BAK1-GFP phosphorylation in Arabidopsis seedlings treated with different concentrations of glucose for 4 h from e . The ratio value of phosphorylated BAK1-GFP to immunoprecipitated BAK1-GFP was set at 1. Values for glucose treatments are given as mean ± SD ( n = 3). ** P < 0.01 compared with the value for 0% glucose treatment by Student’s t -test
Anti Bri1, supplied by Abiocode Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti bri1/product/Abiocode Inc
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
anti bri1 - by Bioz Stars, 2024-04
86/100 stars

Images

1) Product Images from "BRI1 and BAK1 interact with G proteins and regulate sugar-responsive growth and development in Arabidopsis"

Article Title: BRI1 and BAK1 interact with G proteins and regulate sugar-responsive growth and development in Arabidopsis

Journal: Nature Communications

doi: 10.1038/s41467-018-03884-8

Glucose influences the interactions between BRI1 and BAK1. a Glucose regulates the physical interactions between BRI1 and BAK1. pBRI1:BRI1-GFP ; 35S:BAK1 - HA or 35S:BAK1-HA seedlings were grown in the light for 5 days, then incubated in darkness for 4 days, and treated with different concentrations of glucose (G) for 4 or 24 h. IN input; IP immunoprecipitation; IB immunoblot. b Quantification of BRI1-GFP and BAK1-HA associations in Arabidopsis seedlings treated with different concentrations of glucose (G) for 4 h from a . The ratio value of immunoprecipitated BAK1-HA to input BAK1-HA in response to 0% glucose was set at 1. Values for glucose treatments are given as mean ± SD ( n = 3). ** P < 0.01 compared with the value for 0% glucose treatment by Student’s t -test. c Glucose influences the phosphorylation levels of BRI1 in Arabidopsis. pBRI1:BRI1-GFP seedlings were grown in the light for 5 days, then incubated in darkness for 4 days, and treated with different concentrations of glucose (G) for 4 or 24 h. IN input; IP immunoprecipitation; IB immunoblot. d Quantification of BRI1-GFP phosphorylation in Arabidopsis seedlings treated with different concentrations of glucose for 4 h from c . The ratio value of phosphorylated BRI1-GFP to immunoprecipitated BRI1-GFP in response to 0% glucose was set at 1. Values for glucose treatments are given as mean ± SD ( n = 3). ** P < 0.01 compared with the value for 0% glucose treatment by Student’s t -test. e Glucose influences the phosphorylation levels of BAK1 in Arabidopsis. pBAK1:BAK1-GFP seedlings were grown in the light for 5 days, then incubated in darkness for 4 days, and treated with different concentrations of glucose (G) for 4 or 24 h. IN, input; IP immunoprecipitation; IB immunoblot. f Quantification of BAK1-GFP phosphorylation in Arabidopsis seedlings treated with different concentrations of glucose for 4 h from e . The ratio value of phosphorylated BAK1-GFP to immunoprecipitated BAK1-GFP was set at 1. Values for glucose treatments are given as mean ± SD ( n = 3). ** P < 0.01 compared with the value for 0% glucose treatment by Student’s t -test
Figure Legend Snippet: Glucose influences the interactions between BRI1 and BAK1. a Glucose regulates the physical interactions between BRI1 and BAK1. pBRI1:BRI1-GFP ; 35S:BAK1 - HA or 35S:BAK1-HA seedlings were grown in the light for 5 days, then incubated in darkness for 4 days, and treated with different concentrations of glucose (G) for 4 or 24 h. IN input; IP immunoprecipitation; IB immunoblot. b Quantification of BRI1-GFP and BAK1-HA associations in Arabidopsis seedlings treated with different concentrations of glucose (G) for 4 h from a . The ratio value of immunoprecipitated BAK1-HA to input BAK1-HA in response to 0% glucose was set at 1. Values for glucose treatments are given as mean ± SD ( n = 3). ** P < 0.01 compared with the value for 0% glucose treatment by Student’s t -test. c Glucose influences the phosphorylation levels of BRI1 in Arabidopsis. pBRI1:BRI1-GFP seedlings were grown in the light for 5 days, then incubated in darkness for 4 days, and treated with different concentrations of glucose (G) for 4 or 24 h. IN input; IP immunoprecipitation; IB immunoblot. d Quantification of BRI1-GFP phosphorylation in Arabidopsis seedlings treated with different concentrations of glucose for 4 h from c . The ratio value of phosphorylated BRI1-GFP to immunoprecipitated BRI1-GFP in response to 0% glucose was set at 1. Values for glucose treatments are given as mean ± SD ( n = 3). ** P < 0.01 compared with the value for 0% glucose treatment by Student’s t -test. e Glucose influences the phosphorylation levels of BAK1 in Arabidopsis. pBAK1:BAK1-GFP seedlings were grown in the light for 5 days, then incubated in darkness for 4 days, and treated with different concentrations of glucose (G) for 4 or 24 h. IN, input; IP immunoprecipitation; IB immunoblot. f Quantification of BAK1-GFP phosphorylation in Arabidopsis seedlings treated with different concentrations of glucose for 4 h from e . The ratio value of phosphorylated BAK1-GFP to immunoprecipitated BAK1-GFP was set at 1. Values for glucose treatments are given as mean ± SD ( n = 3). ** P < 0.01 compared with the value for 0% glucose treatment by Student’s t -test

Techniques Used: Incubation, Immunoprecipitation, Western Blot

G-proteins act genetically with BRI1 and BAK1. a Comparison of development stages of Col-0 (1), bri1-301 (2), bak1-4 (3), agb1-2 (4), agb1-2 bri1-301 (5), agb1-2 bak1-4 (6), gpa1-101 (7), gpa1-101 bri1-301 (8), gpa1-101 bak1-4 (9), agg3-3 (10), agg3-3 bri1-301 , (11), and agg3-3 bak1-4 (12). Seedlings were grown vertically on medium supplemented with 1% glucose in the dark for 19 days ( n ≥ 85). b The percentage of the indicated seedlings with green cotyledons. Seedlings were grown on medium supplemented with 6% glucose under constant light condition for 16 days ( n ≥ 72). Values in a.b are given as mean ± SD
Figure Legend Snippet: G-proteins act genetically with BRI1 and BAK1. a Comparison of development stages of Col-0 (1), bri1-301 (2), bak1-4 (3), agb1-2 (4), agb1-2 bri1-301 (5), agb1-2 bak1-4 (6), gpa1-101 (7), gpa1-101 bri1-301 (8), gpa1-101 bak1-4 (9), agg3-3 (10), agg3-3 bri1-301 , (11), and agg3-3 bak1-4 (12). Seedlings were grown vertically on medium supplemented with 1% glucose in the dark for 19 days ( n ≥ 85). b The percentage of the indicated seedlings with green cotyledons. Seedlings were grown on medium supplemented with 6% glucose under constant light condition for 16 days ( n ≥ 72). Values in a.b are given as mean ± SD

Techniques Used:

BRI1 and BAK1 physically interact with G proteins. a BRI1 kinase domain (BRI1-KD) interacts with AGB1 and AGG3 in vitro. MBP-GPA1, MBP-AGB1, or MBP-AGG3 was pulled down by GST-BRI1-KD and detected by immunoblot with anti-MBP antibody. Arrow indicates the band of MBP-GPA1.IN, input; IB, immunoblot. b BAK1 kinase domain (BAK1-KD) interacts with AGG3 in vitro. MBP-GPA1, MBP-AGB1, or MBP-AGG3 was pulled down by GST-BAK1-KD and detected by immunoblot with anti-MBP antibody. Arrow indicates the band of MBP-GPA1. IN, input; IB immunoblot. c Bimolecular fluorescence complementation (BiFC) assays show that BRI1 interacts with AGB1 and AGG3 in N . benthamiana leaves. BRI1-nYFP was coexpressed with GPA1-cYFP, AGB1-cYFP, or AGG3-cYFP in leaves of N . benthamiana . d BiFC assays show that BAK1 interacts with AGG3 in N . benthamiana leaves. BAK1-nYFP was coexpressed with GPA1-cYFP, AGB1-cYFP, or AGG3-cYFP in leaves of N . benthamiana . e BRI1 interacts with AGB1 in Arabidopsis. The extracted total proteins from 35S:GFP ; 35S:Myc-AGB1 , or pBRI1:BRI1-GFP ; 35S:Myc-AGB1 seedlings were incubated with GFP-Trap-A, respectively. Immunoprecipitates were examined using anti-Myc and anti-GFP antibodies, respectively. IP immunoprecipitation; IN input; IB immunoblot. f BRI1 interacts with AGG3 in Arabidopsis. The extracted total proteins from pBRI1:BRI1-GFP ; 35S:GFP or pBRI1:BRI1-GFP ; 35S:Myc-AGG3 seedlings were incubated with GFP-Trap-A, and examined using anti-Myc and anti-GFP antibodies, respectively. IP immunoprecipitation; IN input; IB immunoblot. g BAK1 interacts with AGG3 in Arabidopsis. The extracted total proteins from 35S:GFP ; 35S:BAK1-HA and 35S:GFP-AGG3 ; 35S:BAK1-HA seedlings were incubated with GFP-Trap-A, and examined using anti-HA and anti-GFP antibodies, respectively. IP immunoprecipitation; IN input; IB immunoblot. Scale bars, 50 µm in c , d
Figure Legend Snippet: BRI1 and BAK1 physically interact with G proteins. a BRI1 kinase domain (BRI1-KD) interacts with AGB1 and AGG3 in vitro. MBP-GPA1, MBP-AGB1, or MBP-AGG3 was pulled down by GST-BRI1-KD and detected by immunoblot with anti-MBP antibody. Arrow indicates the band of MBP-GPA1.IN, input; IB, immunoblot. b BAK1 kinase domain (BAK1-KD) interacts with AGG3 in vitro. MBP-GPA1, MBP-AGB1, or MBP-AGG3 was pulled down by GST-BAK1-KD and detected by immunoblot with anti-MBP antibody. Arrow indicates the band of MBP-GPA1. IN, input; IB immunoblot. c Bimolecular fluorescence complementation (BiFC) assays show that BRI1 interacts with AGB1 and AGG3 in N . benthamiana leaves. BRI1-nYFP was coexpressed with GPA1-cYFP, AGB1-cYFP, or AGG3-cYFP in leaves of N . benthamiana . d BiFC assays show that BAK1 interacts with AGG3 in N . benthamiana leaves. BAK1-nYFP was coexpressed with GPA1-cYFP, AGB1-cYFP, or AGG3-cYFP in leaves of N . benthamiana . e BRI1 interacts with AGB1 in Arabidopsis. The extracted total proteins from 35S:GFP ; 35S:Myc-AGB1 , or pBRI1:BRI1-GFP ; 35S:Myc-AGB1 seedlings were incubated with GFP-Trap-A, respectively. Immunoprecipitates were examined using anti-Myc and anti-GFP antibodies, respectively. IP immunoprecipitation; IN input; IB immunoblot. f BRI1 interacts with AGG3 in Arabidopsis. The extracted total proteins from pBRI1:BRI1-GFP ; 35S:GFP or pBRI1:BRI1-GFP ; 35S:Myc-AGG3 seedlings were incubated with GFP-Trap-A, and examined using anti-Myc and anti-GFP antibodies, respectively. IP immunoprecipitation; IN input; IB immunoblot. g BAK1 interacts with AGG3 in Arabidopsis. The extracted total proteins from 35S:GFP ; 35S:BAK1-HA and 35S:GFP-AGG3 ; 35S:BAK1-HA seedlings were incubated with GFP-Trap-A, and examined using anti-HA and anti-GFP antibodies, respectively. IP immunoprecipitation; IN input; IB immunoblot. Scale bars, 50 µm in c , d

Techniques Used: In Vitro, Western Blot, Fluorescence, Incubation, Immunoprecipitation

BRI1 is required for the phosphorylation of AGB1 and AGG3. a BRI1 kinase domain (BRI1-KD) phosphorylates AGB1 in vitro, but bri1-301 kinase domain (bri1-301-KD) does not. The phosphorylated MBP-AGB1 (pMBP-AGB1), unphosphorylated (MBP-AGB1) and autophosphorylated GST-BRI1-KD (pGST-BRI1-KD) were separated by phos-tag SDS-PAGE. IN, input. b BRI1-KD phosphorylates AGG3 in vitro, but bri1-301-KD does not. The phosphorylated MBP-AGG3 (pMBP-AGG3), unphosphorylated (MBP-AGG3) and autophosphorylated GST-BRI1-KD (pGST-BRI1-KD) were separated by phos-tag SDS-PAGE. IN, input. c , d BRI1 is required for the phosphorylation of AGB1 in Arabidopsis . Total proteins from pBRI1:BRI1-GFP ; 35S:Myc-AGB1 (L1) and 35S:GFP ; 35S:Myc-AGB1 (L2) seedlings were mixed with anti-Myc-Tag mouse mAb conjugated agarose beads, and detected using anti-Myc and anti-phosphothreonine (pThr) antibodies, respectively c . Quantification of the phosphorylated Myc-AGB1 was relative to the immunoprecipitated Myc-AGB1 d . Values are given as mean ± SD ( n = 3). ** P < 0.01 compared with the value for L2 seedlings by Student’s t -test. e , f BRI1 is required for the phosphorylation of AGG3 in Arabidopsis . Total proteins from pBRI1:BRI1-GFP ; 35S:Myc-AGG3 (L3) and 35S:GFP ; 35S:Myc-AGG3 (L4) seedlings were mixed with anti-Myc-Tag mouse mAb conjugated agarose beads and detected using anti-Myc and anti-phosphothreonine (pThr) antibodies, respectively ( e ). Quantification of the phosphorylated Myc-AGG3 was relative to the immunoprecipitated Myc-AGG3 f . Values are given as mean ± SD ( n = 3).** P < 0.01 compared with the value for L4 seedlings by Student’s t -test. g , h BRI1 is required for the phosphorylation of AGB1 in Arabidopsis . Total proteins from 35S:GFP-AGB1 (L5) and 35S:GFP-AGB1 ; bri1-301 (L6) seedlings were incubated with GFP-Trap-A, and detected using anti-GFP and anti-phosphothreonine (pThr) antibodies, respectively g . Quantification of the phosphorylated GFP-AGB1 was relative to the immunoprecipitated GFP-AGB1 h . Values are given as mean ± SD, ( n = 3).** P < 0.01 compared with the value for L5 seedlings by Student’s t -test. i , j BRI1 is required for the phosphorylation of AGG3 in Arabidopsis . Total proteins from 35S:GFP-AGG3 (L7) and 35S:GFP-AGG3 ; bri1-301 (L8) seedlings were incubated with GFP beads, and detected by immunoblot with anti-GFP and anti-phosphothreonine (pThr) antibodies, respectively i . Quantification of the phosphorylated GFP-AGG3 was relative to the immunoprecipitated GFP-AGG3 j . Values are given as mean ± SD ( n = 3).** P < 0.01 compared with the value for L7 seedlings by Student’s t -test
Figure Legend Snippet: BRI1 is required for the phosphorylation of AGB1 and AGG3. a BRI1 kinase domain (BRI1-KD) phosphorylates AGB1 in vitro, but bri1-301 kinase domain (bri1-301-KD) does not. The phosphorylated MBP-AGB1 (pMBP-AGB1), unphosphorylated (MBP-AGB1) and autophosphorylated GST-BRI1-KD (pGST-BRI1-KD) were separated by phos-tag SDS-PAGE. IN, input. b BRI1-KD phosphorylates AGG3 in vitro, but bri1-301-KD does not. The phosphorylated MBP-AGG3 (pMBP-AGG3), unphosphorylated (MBP-AGG3) and autophosphorylated GST-BRI1-KD (pGST-BRI1-KD) were separated by phos-tag SDS-PAGE. IN, input. c , d BRI1 is required for the phosphorylation of AGB1 in Arabidopsis . Total proteins from pBRI1:BRI1-GFP ; 35S:Myc-AGB1 (L1) and 35S:GFP ; 35S:Myc-AGB1 (L2) seedlings were mixed with anti-Myc-Tag mouse mAb conjugated agarose beads, and detected using anti-Myc and anti-phosphothreonine (pThr) antibodies, respectively c . Quantification of the phosphorylated Myc-AGB1 was relative to the immunoprecipitated Myc-AGB1 d . Values are given as mean ± SD ( n = 3). ** P < 0.01 compared with the value for L2 seedlings by Student’s t -test. e , f BRI1 is required for the phosphorylation of AGG3 in Arabidopsis . Total proteins from pBRI1:BRI1-GFP ; 35S:Myc-AGG3 (L3) and 35S:GFP ; 35S:Myc-AGG3 (L4) seedlings were mixed with anti-Myc-Tag mouse mAb conjugated agarose beads and detected using anti-Myc and anti-phosphothreonine (pThr) antibodies, respectively ( e ). Quantification of the phosphorylated Myc-AGG3 was relative to the immunoprecipitated Myc-AGG3 f . Values are given as mean ± SD ( n = 3).** P < 0.01 compared with the value for L4 seedlings by Student’s t -test. g , h BRI1 is required for the phosphorylation of AGB1 in Arabidopsis . Total proteins from 35S:GFP-AGB1 (L5) and 35S:GFP-AGB1 ; bri1-301 (L6) seedlings were incubated with GFP-Trap-A, and detected using anti-GFP and anti-phosphothreonine (pThr) antibodies, respectively g . Quantification of the phosphorylated GFP-AGB1 was relative to the immunoprecipitated GFP-AGB1 h . Values are given as mean ± SD, ( n = 3).** P < 0.01 compared with the value for L5 seedlings by Student’s t -test. i , j BRI1 is required for the phosphorylation of AGG3 in Arabidopsis . Total proteins from 35S:GFP-AGG3 (L7) and 35S:GFP-AGG3 ; bri1-301 (L8) seedlings were incubated with GFP beads, and detected by immunoblot with anti-GFP and anti-phosphothreonine (pThr) antibodies, respectively i . Quantification of the phosphorylated GFP-AGG3 was relative to the immunoprecipitated GFP-AGG3 j . Values are given as mean ± SD ( n = 3).** P < 0.01 compared with the value for L7 seedlings by Student’s t -test

Techniques Used: In Vitro, SDS Page, Immunoprecipitation, Incubation, Western Blot

BRI1 and BAK1 affect the interactions between GPA1 and AGB1. a BRI1 and BAK1 influences the interactions between GPA1 and AGB1 in Arabidopsis , respectively. The wild type, bri1-301 , and bak1-4 leaf protoplasts co-transformed with 35S:AGB1 - Myc and 35S : GPA1 - HA plasmids were grown in darkness for 14 h, and then treated without or with 2% glucose (G) for 5 h. Total proteins from these leaf protoplasts were incubated with anti-Myc-Tag mouse mAb conjugated agarose beads, and detected using anti-Myc and anti-HA antibodies, respectively. IN input; IP immunoprecipitation. b – d Quantification of relative interactions between GPA1 and AGB1 in wild type ( b ), bri1-301 ( c ) and bak1-4 ( d ) leaf protoplasts treated without or with 2% glucose as shown in a . The ratio was shown as immunoprecipitated GPA1-HA to input GPA1-HA. Values are given as mean ± SD ( n = 3) relative to the value for 0% glucose, set at 1. Values in b – d are given as mean ± SD, ** P < 0.01 compared with the value for 0% glucose treatment by Student’s t -test
Figure Legend Snippet: BRI1 and BAK1 affect the interactions between GPA1 and AGB1. a BRI1 and BAK1 influences the interactions between GPA1 and AGB1 in Arabidopsis , respectively. The wild type, bri1-301 , and bak1-4 leaf protoplasts co-transformed with 35S:AGB1 - Myc and 35S : GPA1 - HA plasmids were grown in darkness for 14 h, and then treated without or with 2% glucose (G) for 5 h. Total proteins from these leaf protoplasts were incubated with anti-Myc-Tag mouse mAb conjugated agarose beads, and detected using anti-Myc and anti-HA antibodies, respectively. IN input; IP immunoprecipitation. b – d Quantification of relative interactions between GPA1 and AGB1 in wild type ( b ), bri1-301 ( c ) and bak1-4 ( d ) leaf protoplasts treated without or with 2% glucose as shown in a . The ratio was shown as immunoprecipitated GPA1-HA to input GPA1-HA. Values are given as mean ± SD ( n = 3) relative to the value for 0% glucose, set at 1. Values in b – d are given as mean ± SD, ** P < 0.01 compared with the value for 0% glucose treatment by Student’s t -test

Techniques Used: Transformation Assay, Incubation, Immunoprecipitation

BRI1 and BAK1 play key roles in sugar responses. a Different stages of dark-grown wild-type seedlings used for scoring sugar responses. b Comparison of developmental stages between Col-0, bri1-301 , and bak1-4 . Seedlings were grown vertically on medium supplemented with 1% glucose (G) or 1% mannitol (M) in the dark for 19 days ( n ≥ 83). c The percentage of Col-0, bri1-301 , and bak1-4 seedlings with green cotyledons. Seedlings were grown on medium supplemented with 6% glucose (G) or 6% mannitol (M) under constant light condition for 10 days ( n ≥ 89). Values in b , c are given as mean ± SD. ** P < 0.01 compared with the wild type by Student’s t -test. Scale bars, 2 mm ( a )
Figure Legend Snippet: BRI1 and BAK1 play key roles in sugar responses. a Different stages of dark-grown wild-type seedlings used for scoring sugar responses. b Comparison of developmental stages between Col-0, bri1-301 , and bak1-4 . Seedlings were grown vertically on medium supplemented with 1% glucose (G) or 1% mannitol (M) in the dark for 19 days ( n ≥ 83). c The percentage of Col-0, bri1-301 , and bak1-4 seedlings with green cotyledons. Seedlings were grown on medium supplemented with 6% glucose (G) or 6% mannitol (M) under constant light condition for 10 days ( n ≥ 89). Values in b , c are given as mean ± SD. ** P < 0.01 compared with the wild type by Student’s t -test. Scale bars, 2 mm ( a )

Techniques Used:

Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86
    Abiocode Inc anti bri1
    Anti Bri1, supplied by Abiocode Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti bri1/product/Abiocode Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti bri1 - by Bioz Stars, 2024-04
    86/100 stars
      Buy from Supplier

    Image Search Results