anti br utp mab (Millipore)

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Name:

Monoclonal Anti hnRNP K J antibody

Description:

Catalog Number:

r8903

Price:

None

Applications:

The antibody may be used in various immunochemical techniques including enzyme linked immunosorbent assay (ELISA), immunoblotting, chromatin immunoprecipitation (ChIP), immunocytochemistry, antibody-based supershift assay and electrophoretic mobility shift assay (EMSA).

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Structured Review

Millipore anti br utp mab
Monoclonal Anti hnRNP K J antibody

Monoclonal Anti hnRNP K J mouse IgG2b isotype is derived from the 3C2 hybridoma produced by the fusion of mouse myeloma cells SP2 0 cells and splenocytes from BALB c mice immunized with hnRNP s purified by oligo dC affinity chromatography
https://www.bioz.com/result/anti br utp mab/product/Millipore
Average 85 stars, based on 1 article reviews
Price from $9.99 to $1999.99

anti br utp mab - by Bioz Stars, 2021-03

85/100 stars

Images

1) Product Images from "Initiation of Nucleolar Assembly Is Independent of RNA Polymerase I Transcription"

Article Title: Initiation of Nucleolar Assembly Is Independent of RNA Polymerase I Transcription

Journal: Molecular Biology of the Cell

doi:

ActD at a concentration of 0.04 μg/ml selectively inhibits Pol I transcription without significantly affecting other polymerases and general nuclear organization. A and A′ show the in situ incorporation of Br-UTP, and B and B′ show the same cells corresponding to those in A and A′ immunolabeled with anti-fibrillarin antibody. Daughter cells grown in the presence of ActD form mininucleoli that are immunolabeled with anti-fibrillarin antibody (B, arrows). They do not incorporate Br-UTP (A, arrows), whereas untreated nucleoli (B′, arrows) actively incorporate Br-UTP after pulse labeling (A′). Although Br-UTP incorporation in nucleoli declines in treated cells, the nucleoplasmic Br-UTP incorporation from Pol II and Pol III transcription is not altered (A). The speckled distribution of SC35 (C) and the predominantly nuclear localization of hnRNP A1 (D) are also not changed significantly in treated cells. Bar, 10 μm.

Figure Legend Snippet: ActD at a concentration of 0.04 μg/ml selectively inhibits Pol I transcription without significantly affecting other polymerases and general nuclear organization. A and A′ show the in situ incorporation of Br-UTP, and B and B′ show the same cells corresponding to those in A and A′ immunolabeled with anti-fibrillarin antibody. Daughter cells grown in the presence of ActD form mininucleoli that are immunolabeled with anti-fibrillarin antibody (B, arrows). They do not incorporate Br-UTP (A, arrows), whereas untreated nucleoli (B′, arrows) actively incorporate Br-UTP after pulse labeling (A′). Although Br-UTP incorporation in nucleoli declines in treated cells, the nucleoplasmic Br-UTP incorporation from Pol II and Pol III transcription is not altered (A). The speckled distribution of SC35 (C) and the predominantly nuclear localization of hnRNP A1 (D) are also not changed significantly in treated cells. Bar, 10 μm.

Techniques Used: Concentration Assay, In Situ, Immunolabeling, Labeling

Western Blot:

Article Title: A Proteomic Approach To Identify Candidate Substrates of Human Adenovirus E4orf6-E1B55K and Other Viral Cullin-Based E3 Ubiquitin Ligases ▿
Article Snippet: .. The following antibodies were used: rabbit anti-E4orf6 (1807) , rabbit anti-Mre11 (catalog no. pNB 100-142; Novus Biologicals, Inc.), rabbit anti-Cul5 H-300 (catalog no. sc-13014; Santa Cruz) and rabbit anti-tryptophanyl tRNA synthetase (catalog no. ab31536; AbCam); mouse anti-E1B55K clone 2A6 (Western blots) ( ) or rat monoclonal 7C11 (immunofluorescence) , mouse anti-integrin alpha3A clone 29A3 (catalog no. MAB2290; Millipore), mouse anti-actin clone C4 (catalog no. 691001; MP Biomedicals), mouse anti-cortactin clone 4F11 (a generous gift from J. Lavoie, Université Laval), mouse anti-MEK2 clone 96 (catalog no. 610235; BD Biosciences), mouse anti-dynactin clone 1 (catalog no. 610473; BD Biosciences), mouse anti-lamin B2 clone LN43 (catalog no. MAB3536; Millipore), mouse anti-dynein clone 70.1 (catalog no. D5167; Sigma), mouse anti-hnRNP-K/J clone 3C2 (catalog no. R8903; Sigma), mouse anti-c-Myc clone 9E10 (catalog no. MMS-150R; Covance), and mouse anti-E2A DNA-binding protein (DBP) B6-8 ( ). .. Secondary antibodies conjugated to horseradish peroxidase for detection in Western blotting analyses were goat anti-mouse and goat anti-rabbit immunoglobulin G (Jackson Immunoresearch Laboratories).

Immunofluorescence:

Article Title: Inhibition of Nuclear Import and Alteration of Nuclear Pore Complex Composition by Rhinovirus
Article Snippet: DNA transfections were performed with the Lipofectin reagent following the manufacturer's recommendations (Gibco/BRL). .. The following antibodies were used for indirect immunofluorescence: MS3 to detect nucleolin , SW5 to detect La , #SC-333 to detect Sam68 (Santa Cruz Biotechnology), 9H10 to detect heterogeneous nuclear ribonucleoprotein (hnRNP) A1 (a gift from G. Dreyfuss), 12G4 to detect hnRNP K/J , 4F4 to detect hnRNP C , #S-4045 to detect SC35 (Sigma), 414 to detect nucleoporins (Convance), and #SC-7292 to detect lamins A and C (Santa Cruz Biotechnology). ..

Article Title: Effects of poliovirus infection on nucleo-cytoplasmic trafficking and nuclear pore complex composition
Article Snippet: The resulting plasmid, pEGFP-NLS, contains four tandem repeats of the TAg NLS (PKKKRKV). pGEX-NLS-GFP encodes a GST–NLS–GFP fusion protein ( ). pXRGG encodes a fusion protein consisting of the full-length HIV-1 Rev protein, linked to the rat glucocorticoid hormone binding domain and GFP ( ). .. Mouse monoclonal antibodies used for indirect immunofluorescence analysis included: 9H10 to detect hnRNP A1, 12G4 to detect hnRNP K/J, 4F4 to detect hnRNP C, #S-4045 (Sigma) to detect SC35, 414 (Convance) to detect nucleoporins and #SC-7292 (Santa Cruz Biotechnology) to detect lamins A and C. Before fixation, cells were washed three times with phosphate-buffered saline (PBS). .. Immunostaining for hnRNP A1, hnRNP K/J, hnRNP C and SC35 was accomplished by fixing cells in 2% formaldehyde for 30 min at 25°C, washing three times with PBS and permeabilizing in acetone at –20°C for 3 min. Immunostaining using antibodies 414 and #SC-7292 was carried out by fixing cells in 3% formaldehyde for 20 min at 25°C, washing three times with PBS and permeabilizing in methanol at –20°C for 5 min.

Binding Assay:

Article Title: Progesterone receptor downregulates breast cancer resistance protein expression via binding to the progesterone response element in breast cancer
Article Snippet: Specific binding was inhibited by the addition of 20‐ and 50‐fold excess of unlabeled probe or 50‐fold excess of unlabeled mutERE probe. .. For antibody supershift assay, 2 μL of anti‐PR antibody was added in the binding reaction. .. Nondenaturing 5% polyacrylamide gels were pre‐electrophoresed for 40 min in 0.5×Tris borate‐EDTA (TBE) buffer before loading the binding reaction samples.

Chloramphenicol Acetyltransferase Assay:

Article Title: TNF-α Suppresses Prolyl-4-Hydroxylase α1 Expression via the ASK1-JNK-NonO Pathway
Article Snippet: The blot was then washed, and chemiluminescent detection was performed with CSPD as the substrate in the ECL kit (Pierce) before being exposed to X-ray film. .. For the antibody-based supershift assay, antibodies against transcription factors, including NonO (0.2 μ g, CatA300-587A, Lot#A300-587A-1, Bethyl, Montgomery, Tex), hnRNP-K (2 μ g, Cat#: R8903, Sigma), AP2 (0.5 μ g, Cat#: KAP-TF100, Nventa, Victoria, BC), and v-Myb/c-Myb (1 μ g, Cat#: GTX10935 GeneTex) were added to a mixture of biotin-labeled oligonucleotide probes and nuclear proteins that had been reacted for 30 minutes. .. After an additional 30 minutes of incubation, the protein-DNA mixture was electrophoresized in 6% polyacrylamide gel for 1 hour.