anti br utp mab  (Millipore)


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    Name:
    Monoclonal Antibodies
    Description:
    Considers the preparation testing derivation and applications of monoclonal antibodies New immunological techniques incorporating tried and tested methodologies are described Both the standard somatic hybridization technique and recombinant techniques are described along with both small and large scale production purification and labeling Protocols are given for the use of monoclonal antibodies in rheumatoid arthritis tissue typing detecting DNA modified during chemotherapy and in the clinical analysis of transplantation samples for malignancy
    Catalog Number:
    m4559
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    None
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    Structured Review

    Millipore anti br utp mab
    Monoclonal Antibodies
    Considers the preparation testing derivation and applications of monoclonal antibodies New immunological techniques incorporating tried and tested methodologies are described Both the standard somatic hybridization technique and recombinant techniques are described along with both small and large scale production purification and labeling Protocols are given for the use of monoclonal antibodies in rheumatoid arthritis tissue typing detecting DNA modified during chemotherapy and in the clinical analysis of transplantation samples for malignancy
    https://www.bioz.com/result/anti br utp mab/product/Millipore
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti br utp mab - by Bioz Stars, 2020-09
    99/100 stars

    Images

    1) Product Images from "Initiation of Nucleolar Assembly Is Independent of RNA Polymerase I Transcription"

    Article Title: Initiation of Nucleolar Assembly Is Independent of RNA Polymerase I Transcription

    Journal: Molecular Biology of the Cell

    doi:

    ActD at a concentration of 0.04 μg/ml selectively inhibits Pol I transcription without significantly affecting other polymerases and general nuclear organization. A and A′ show the in situ incorporation of Br-UTP, and B and B′ show the same cells corresponding to those in A and A′ immunolabeled with anti-fibrillarin antibody. Daughter cells grown in the presence of ActD form mininucleoli that are immunolabeled with anti-fibrillarin antibody (B, arrows). They do not incorporate Br-UTP (A, arrows), whereas untreated nucleoli (B′, arrows) actively incorporate Br-UTP after pulse labeling (A′). Although Br-UTP incorporation in nucleoli declines in treated cells, the nucleoplasmic Br-UTP incorporation from Pol II and Pol III transcription is not altered (A). The speckled distribution of SC35 (C) and the predominantly nuclear localization of hnRNP A1 (D) are also not changed significantly in treated cells. Bar, 10 μm.
    Figure Legend Snippet: ActD at a concentration of 0.04 μg/ml selectively inhibits Pol I transcription without significantly affecting other polymerases and general nuclear organization. A and A′ show the in situ incorporation of Br-UTP, and B and B′ show the same cells corresponding to those in A and A′ immunolabeled with anti-fibrillarin antibody. Daughter cells grown in the presence of ActD form mininucleoli that are immunolabeled with anti-fibrillarin antibody (B, arrows). They do not incorporate Br-UTP (A, arrows), whereas untreated nucleoli (B′, arrows) actively incorporate Br-UTP after pulse labeling (A′). Although Br-UTP incorporation in nucleoli declines in treated cells, the nucleoplasmic Br-UTP incorporation from Pol II and Pol III transcription is not altered (A). The speckled distribution of SC35 (C) and the predominantly nuclear localization of hnRNP A1 (D) are also not changed significantly in treated cells. Bar, 10 μm.

    Techniques Used: Concentration Assay, In Situ, Immunolabeling, Labeling

    Related Articles

    Western Blot:

    Article Title: Human CLEC18 Gene Cluster Contains C-type Lectins with Differential Glycan-binding Specificity *
    Article Snippet: .. The immunoprecipitates were fractionated on a 12% SDS-PAGE, then probed with anti-CLEC18 mAb (clone 3A9E6), and further incubated with peroxidase-conjugated goat anti-mouse polyclonal antibody (Millpore AP181P) and ImmobilonTM Western Chemiluminescent HRP Substrate (MilliporeTM ) subsequently for detection. .. Alternatively, the immunoprecipitates were fractionated on 12% SDS-PAGE, then eluted from gel for mass spectrometry analysis.

    Incubation:

    Article Title: Steady-state dynamics of Cajal body components in the Xenopus germinal vesicle
    Article Snippet: .. GV spreads were incubated with 10 μl of 1 μg/ml mAb K121 (Oncogene Research Products) for 1 h at RT. .. Slides were washed 3–5 min with PBS and incubated for 1 h with 10 μl of 1 μg/ml Alexa 488–labeled goat anti–mouse IgG (Molecular Probes, Inc.).

    Article Title: Human CLEC18 Gene Cluster Contains C-type Lectins with Differential Glycan-binding Specificity *
    Article Snippet: .. The immunoprecipitates were fractionated on a 12% SDS-PAGE, then probed with anti-CLEC18 mAb (clone 3A9E6), and further incubated with peroxidase-conjugated goat anti-mouse polyclonal antibody (Millpore AP181P) and ImmobilonTM Western Chemiluminescent HRP Substrate (MilliporeTM ) subsequently for detection. .. Alternatively, the immunoprecipitates were fractionated on 12% SDS-PAGE, then eluted from gel for mass spectrometry analysis.

    Article Title: Combined Immune Therapy for the Treatment of Visceral Leishmaniasis
    Article Snippet: .. The remaining sample was plated in duplicate (300μl) in 96 well tissue culture plates (Thermo Fisher, Waltham, MA) and incubated for 3 days at 37°C and 5% CO2 in presence of either anti-GITR mAb (clone TRX518; 20μg/ml, Tolerx, Cambridge, MA) or isotype control (human IgG1;Sigma, St Louis, MO). .. TRX518 is a non-depleting, humanized IgG1 anti-human GITR mAb with a heavy chain asparagine 297 substitution to alanine to eliminate N-linked glycolsylation and abrogate Fc region functionality[ ], produced under good manufacturing process (GMP) conditions.

    Article Title: Thermal ablation of tumor cells with antibody-functionalized single-walled carbon nanotubes
    Article Snippet: .. For functional stability, mAb-CNTs, prepared as described above, were suspended in 0.1 ml of mouse serum (Sigma) and incubated at 37°C for 0–72 h. At each time point, the suspension was washed with ice-cold PBS, and the pellet was resuspended in 40 μl of PBS. ..

    Recombinant:

    Article Title: A Membrane-bound eIF2 Alpha Kinase Located in Endosomes Is Regulated by Heme and Controls Differentiation and ROS Levels in Trypanosoma cruzi
    Article Snippet: .. A monoclonal antibody (mAb) 5D10, obtained as described in [ ] by immunization with a recombinant protein corresponding to the residue 346 to 511 of T. cruzi K2, and expressed from pET28a (Novagen) in E. coli BL21. ..

    SDS Page:

    Article Title: Human CLEC18 Gene Cluster Contains C-type Lectins with Differential Glycan-binding Specificity *
    Article Snippet: .. The immunoprecipitates were fractionated on a 12% SDS-PAGE, then probed with anti-CLEC18 mAb (clone 3A9E6), and further incubated with peroxidase-conjugated goat anti-mouse polyclonal antibody (Millpore AP181P) and ImmobilonTM Western Chemiluminescent HRP Substrate (MilliporeTM ) subsequently for detection. .. Alternatively, the immunoprecipitates were fractionated on 12% SDS-PAGE, then eluted from gel for mass spectrometry analysis.

    Functional Assay:

    Article Title: Thermal ablation of tumor cells with antibody-functionalized single-walled carbon nanotubes
    Article Snippet: .. For functional stability, mAb-CNTs, prepared as described above, were suspended in 0.1 ml of mouse serum (Sigma) and incubated at 37°C for 0–72 h. At each time point, the suspension was washed with ice-cold PBS, and the pellet was resuspended in 40 μl of PBS. ..

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    Millipore apoptosis detection
    PDCD4 induces turnover (proliferation and <t>apoptosis)</t> of HUVECs. HUVECs were transfected with pEGFP-C1-Mock (Mock), pEGFP-C1-PDCD4 (PDCD4), negative control (NC) or PDCD4 siRNA (si-PDCD4). (A, B) Western blot analysis of protein levels as indicated. Results are representative of 3 independent experiments. (C, D) Confocal laser-scanning microscopy of BrdU by immunofluorescence (magenta). BrdU was added into the medium 6 hr before harvesting. DAPI was used to stain nuclei (blue). (E, F) The percentage of BrdU positive cells was calculated. (G, H) HUVECs with stained nuclei (brown) were considered TUNEL positive (red arrows). (I, J) The percentage of TUNEL positive cells was calculated. Quantitative data in (E, F, I, J) are mean±SEM from 3 independent experiments. * P
    Apoptosis Detection, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 61 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/apoptosis detection/product/Millipore
    Average 99 stars, based on 61 article reviews
    Price from $9.99 to $1999.99
    apoptosis detection - by Bioz Stars, 2020-09
    99/100 stars
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    Image Search Results


    PDCD4 induces turnover (proliferation and apoptosis) of HUVECs. HUVECs were transfected with pEGFP-C1-Mock (Mock), pEGFP-C1-PDCD4 (PDCD4), negative control (NC) or PDCD4 siRNA (si-PDCD4). (A, B) Western blot analysis of protein levels as indicated. Results are representative of 3 independent experiments. (C, D) Confocal laser-scanning microscopy of BrdU by immunofluorescence (magenta). BrdU was added into the medium 6 hr before harvesting. DAPI was used to stain nuclei (blue). (E, F) The percentage of BrdU positive cells was calculated. (G, H) HUVECs with stained nuclei (brown) were considered TUNEL positive (red arrows). (I, J) The percentage of TUNEL positive cells was calculated. Quantitative data in (E, F, I, J) are mean±SEM from 3 independent experiments. * P

    Journal: PLoS ONE

    Article Title: Atheroprotective Pulsatile Flow Induces Ubiquitin-Proteasome-Mediated Degradation of Programmed Cell Death 4 in Endothelial Cells

    doi: 10.1371/journal.pone.0091564

    Figure Lengend Snippet: PDCD4 induces turnover (proliferation and apoptosis) of HUVECs. HUVECs were transfected with pEGFP-C1-Mock (Mock), pEGFP-C1-PDCD4 (PDCD4), negative control (NC) or PDCD4 siRNA (si-PDCD4). (A, B) Western blot analysis of protein levels as indicated. Results are representative of 3 independent experiments. (C, D) Confocal laser-scanning microscopy of BrdU by immunofluorescence (magenta). BrdU was added into the medium 6 hr before harvesting. DAPI was used to stain nuclei (blue). (E, F) The percentage of BrdU positive cells was calculated. (G, H) HUVECs with stained nuclei (brown) were considered TUNEL positive (red arrows). (I, J) The percentage of TUNEL positive cells was calculated. Quantitative data in (E, F, I, J) are mean±SEM from 3 independent experiments. * P

    Article Snippet: Apoptosis Detection (Terminal Deoxynucleotidyl Transferase-mediated UTP Nick End Labeling, TUNEL) Apoptosis was detected using the ApopTag Plus Peroxidase In Situ Apoptosis Detection Kit (Millipore, Billerica, MA, USA).

    Techniques: Transfection, Negative Control, Western Blot, Confocal Laser Scanning Microscopy, Immunofluorescence, Staining, TUNEL Assay