Journal: American Journal of Physiology - Renal Physiology
Article Title: Shear stress-induced volume decrease in C11-MDCK cells by BK-?/?4
Figure Lengend Snippet: Effects of BK-β4 small interfering (si) RNA on shear-stress (10 dynes/cm 2 )-induced K efflux from C11. A : C11 were untreated (normal), transfected with green fluorescent protein (control), 4 nontarget siRNA oligos (siRNA nontarget), or 6 siRNA
Article Snippet: Primary antibodies included anti-BK-α (rabbit polyclonal, diluted 1:1,000, Alomone Labs), anti-BK-β4 (rabbit polyclonal, diluted 1:1,000, Alomone Labs), and anti-actin (rabbit polyclonal, 1:1,000, Santa Cruz Biotechnology, Santa Cruz, CA) with either donkey (Santa Cruz Biotechnology) or goat (Pierce Biotechnology) anti-rabbit IgG-conjugated horseradish peroxidase secondary antibody diluted 1:20,000–1:40,000.