anti asic1  (Alomone Labs)


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    Alomone Labs anti asic1
    <t>ASIC1</t> and ASIC2a protein expression during OGD-Rep in three types of neurons. Cells were treated with OGD-Rep and prepared for immunofluorescence and western blot analysis. A, C. Representative pictures and summary data showing expressions of ASIC1 and ASIC2a using immunofluorescence. B, D. Representative bands and summary data of ASIC1 and ASIC2a expression in western blotting. Data are the mean ± SD in each group. *P
    Anti Asic1, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti asic1/product/Alomone Labs
    Average 94 stars, based on 11 article reviews
    Price from $9.99 to $1999.99
    anti asic1 - by Bioz Stars, 2022-08
    94/100 stars

    Images

    1) Product Images from "Down-regulation of ASICs current and the calcium transients by disrupting PICK1 protects primary cultured mouse cortical neurons from OGD-Rep insults"

    Article Title: Down-regulation of ASICs current and the calcium transients by disrupting PICK1 protects primary cultured mouse cortical neurons from OGD-Rep insults

    Journal: International Journal of Clinical and Experimental Pathology

    doi:

    ASIC1 and ASIC2a protein expression during OGD-Rep in three types of neurons. Cells were treated with OGD-Rep and prepared for immunofluorescence and western blot analysis. A, C. Representative pictures and summary data showing expressions of ASIC1 and ASIC2a using immunofluorescence. B, D. Representative bands and summary data of ASIC1 and ASIC2a expression in western blotting. Data are the mean ± SD in each group. *P
    Figure Legend Snippet: ASIC1 and ASIC2a protein expression during OGD-Rep in three types of neurons. Cells were treated with OGD-Rep and prepared for immunofluorescence and western blot analysis. A, C. Representative pictures and summary data showing expressions of ASIC1 and ASIC2a using immunofluorescence. B, D. Representative bands and summary data of ASIC1 and ASIC2a expression in western blotting. Data are the mean ± SD in each group. *P

    Techniques Used: Expressing, Immunofluorescence, Western Blot

    2) Product Images from "Upregulation of spinal ASIC1 by miR‐485 mediates enterodynia in adult offspring rats with prenatal maternal stress, et al. Upregulation of spinal ASIC1 by miR‐485 mediates enterodynia in adult offspring rats with prenatal maternal stress"

    Article Title: Upregulation of spinal ASIC1 by miR‐485 mediates enterodynia in adult offspring rats with prenatal maternal stress, et al. Upregulation of spinal ASIC1 by miR‐485 mediates enterodynia in adult offspring rats with prenatal maternal stress

    Journal: CNS Neuroscience & Therapeutics

    doi: 10.1111/cns.13542

    ASIC1, involved in the enterodynia of PMS offspring rats, was co‐localization with spinal dorsal horn neurons. (A) PMS significantly reduced the pain threshold of the offspring rats at the age of 6 weeks compared with CON rats ( n = 7 for each group, *** P = 0.0006
    Figure Legend Snippet: ASIC1, involved in the enterodynia of PMS offspring rats, was co‐localization with spinal dorsal horn neurons. (A) PMS significantly reduced the pain threshold of the offspring rats at the age of 6 weeks compared with CON rats ( n = 7 for each group, *** P = 0.0006

    Techniques Used:

    MiR‐485 agomir negatively regulated ASIC1 expression and reversed the synaptic transmission of spinal dorsal horn in PMS offspring rats. (A) The expression of ASIC1 was obviously reduced in spinal dorsal horn after intrathecally injecting miR‐485 agomir for consecutive 7 days ( n = 7 for each group, * P
    Figure Legend Snippet: MiR‐485 agomir negatively regulated ASIC1 expression and reversed the synaptic transmission of spinal dorsal horn in PMS offspring rats. (A) The expression of ASIC1 was obviously reduced in spinal dorsal horn after intrathecally injecting miR‐485 agomir for consecutive 7 days ( n = 7 for each group, * P

    Techniques Used: Expressing, Transmission Assay

    MiR‐485 was reduced in the spinal dorsal horn of PMS offspring rats and co‐expressed with ASIC1 in spinal dorsal horn. (A) PMS significantly reduced miR‐485 expression in T13‐L2 spinal dorsal horn at the age of 6 weeks compared with CON ( n = 4 for each group, ** P
    Figure Legend Snippet: MiR‐485 was reduced in the spinal dorsal horn of PMS offspring rats and co‐expressed with ASIC1 in spinal dorsal horn. (A) PMS significantly reduced miR‐485 expression in T13‐L2 spinal dorsal horn at the age of 6 weeks compared with CON ( n = 4 for each group, ** P

    Techniques Used: Expressing

    3) Product Images from "Deletion of Annexin 2 Light Chain p11 in Nociceptors Causes Deficits in Somatosensory Coding and Pain Behavior"

    Article Title: Deletion of Annexin 2 Light Chain p11 in Nociceptors Causes Deficits in Somatosensory Coding and Pain Behavior

    Journal: The Journal of Neuroscience

    doi: 10.1523/JNEUROSCI.1997-06.2006

    Effects of p11 deletion on cell survival and membrane trafficking of p11-associated proteins. a , Acutely cultured DRG neurons from p11 conditional-null (cKO) and littermate control animals (Wild-type) stained with antibody to p11. Locations of unstained cells in the conditional-null are shown by white arrows, replicated on the adjacent bright-field image. b , Acutely cultured DRG neurons (as in a ) stained with antibodies to p11 (green) and Na V 1.8 (red). Overlap of p11 and Na V 1.8 staining can be seen in cells from littermate control; p11 and Na V 1.8 expression appears mutually exclusive in cells from the cKO animals. c , DRG sections from floxed p11 littermate control (Wild-type) and p11 conditional-null mice (cKO) stained with anti-peripherin (green) and N200 (red). No difference in ratio was observed between littermate control ( n = 11) and p11 conditional-null ( n = 24) sections. Peripherin control (Peri), 67 ± 3%; p11 conditional-null, 64 ± 4%; N-200 control, 37 ± 3%; p11 conditional-null, 38 ± 4%. Error bars represent mean ± SEM. d , Acutely cultured DRG neurons (Wild-type, littermate control; cKO, p11 conditional-null) stained with anti-annexin 2. Membrane localization is evident but appears to be unaffected by p11 deletion. e , Western blots performed on crude DRG plasma membrane preparations from global p11-null (KO) and control (WT) animals, using anti-annexin 2 ( e1 ). No differences were evident (WT 20, 40 μg; KO 20, 40 μg). e2 , Anti-Na V 1.8, using crude plasma membrane preparations. A reduction in membrane levels was observed (WT 20, 40 μg; KO 20, 40 μg). e3 , Anti-Na V 1.8, using total cell lysate. No differences were observed (WT and KO, 400 μg). e4 , Anti-5-HT 1B . No differences in membrane levels were observed (WT and KO, 40 μg). e5 , Anti-ASIC1. No differences in membrane levels were observed (WT and KO, 40 μg).
    Figure Legend Snippet: Effects of p11 deletion on cell survival and membrane trafficking of p11-associated proteins. a , Acutely cultured DRG neurons from p11 conditional-null (cKO) and littermate control animals (Wild-type) stained with antibody to p11. Locations of unstained cells in the conditional-null are shown by white arrows, replicated on the adjacent bright-field image. b , Acutely cultured DRG neurons (as in a ) stained with antibodies to p11 (green) and Na V 1.8 (red). Overlap of p11 and Na V 1.8 staining can be seen in cells from littermate control; p11 and Na V 1.8 expression appears mutually exclusive in cells from the cKO animals. c , DRG sections from floxed p11 littermate control (Wild-type) and p11 conditional-null mice (cKO) stained with anti-peripherin (green) and N200 (red). No difference in ratio was observed between littermate control ( n = 11) and p11 conditional-null ( n = 24) sections. Peripherin control (Peri), 67 ± 3%; p11 conditional-null, 64 ± 4%; N-200 control, 37 ± 3%; p11 conditional-null, 38 ± 4%. Error bars represent mean ± SEM. d , Acutely cultured DRG neurons (Wild-type, littermate control; cKO, p11 conditional-null) stained with anti-annexin 2. Membrane localization is evident but appears to be unaffected by p11 deletion. e , Western blots performed on crude DRG plasma membrane preparations from global p11-null (KO) and control (WT) animals, using anti-annexin 2 ( e1 ). No differences were evident (WT 20, 40 μg; KO 20, 40 μg). e2 , Anti-Na V 1.8, using crude plasma membrane preparations. A reduction in membrane levels was observed (WT 20, 40 μg; KO 20, 40 μg). e3 , Anti-Na V 1.8, using total cell lysate. No differences were observed (WT and KO, 400 μg). e4 , Anti-5-HT 1B . No differences in membrane levels were observed (WT and KO, 40 μg). e5 , Anti-ASIC1. No differences in membrane levels were observed (WT and KO, 40 μg).

    Techniques Used: Cell Culture, Staining, Expressing, Mouse Assay, Western Blot

    4) Product Images from "CO2 chemosensing in rat oesophagus"

    Article Title: CO2 chemosensing in rat oesophagus

    Journal: Gut

    doi: 10.1136/gut.2007.144378

    Expression of acid-sensing ion channel (ASIC) isoforms and transient receptor potential vanilloid 1 (TRPV1) in rat oesophageal mucosa. ASIC1 (A), ASIC2 (D) and ASIC3 (G) were expressed in the prickle cells. ASIC3 was also expressed in the muscularis mucosa
    Figure Legend Snippet: Expression of acid-sensing ion channel (ASIC) isoforms and transient receptor potential vanilloid 1 (TRPV1) in rat oesophageal mucosa. ASIC1 (A), ASIC2 (D) and ASIC3 (G) were expressed in the prickle cells. ASIC3 was also expressed in the muscularis mucosa

    Techniques Used: Expressing

    5) Product Images from "Down-regulation of ASICs current and the calcium transients by disrupting PICK1 protects primary cultured mouse cortical neurons from OGD-Rep insults"

    Article Title: Down-regulation of ASICs current and the calcium transients by disrupting PICK1 protects primary cultured mouse cortical neurons from OGD-Rep insults

    Journal: International Journal of Clinical and Experimental Pathology

    doi:

    ASIC1 and ASIC2a protein expression during OGD-Rep in three types of neurons. Cells were treated with OGD-Rep and prepared for immunofluorescence and western blot analysis. A, C. Representative pictures and summary data showing expressions of ASIC1 and ASIC2a using immunofluorescence. B, D. Representative bands and summary data of ASIC1 and ASIC2a expression in western blotting. Data are the mean ± SD in each group. *P
    Figure Legend Snippet: ASIC1 and ASIC2a protein expression during OGD-Rep in three types of neurons. Cells were treated with OGD-Rep and prepared for immunofluorescence and western blot analysis. A, C. Representative pictures and summary data showing expressions of ASIC1 and ASIC2a using immunofluorescence. B, D. Representative bands and summary data of ASIC1 and ASIC2a expression in western blotting. Data are the mean ± SD in each group. *P

    Techniques Used: Expressing, Immunofluorescence, Western Blot

    6) Product Images from "Upregulation of Spinal ASIC1 and NKCC1 Expression Contributes to Chronic Visceral Pain in Rats"

    Article Title: Upregulation of Spinal ASIC1 and NKCC1 Expression Contributes to Chronic Visceral Pain in Rats

    Journal: Frontiers in Molecular Neuroscience

    doi: 10.3389/fnmol.2020.611179

    Interaction of ASIC1 and NKCC1. (A) ASIC1 and NKCC1 were co-located in the spinal dorsal horn neurons. (B) Expression of NKCC1 in the spinal dorsal horn after intrathecal injection of Amiloride in NMD rats (* P
    Figure Legend Snippet: Interaction of ASIC1 and NKCC1. (A) ASIC1 and NKCC1 were co-located in the spinal dorsal horn neurons. (B) Expression of NKCC1 in the spinal dorsal horn after intrathecal injection of Amiloride in NMD rats (* P

    Techniques Used: Expressing, Injection

    NMD enhanced acid-sensing ion channel 1 (ASIC1) expression. (A) Expression of ASIC1 in the spinal dorsal horn of T13-L2 in NMD rats compared with CON rats (* P
    Figure Legend Snippet: NMD enhanced acid-sensing ion channel 1 (ASIC1) expression. (A) Expression of ASIC1 in the spinal dorsal horn of T13-L2 in NMD rats compared with CON rats (* P

    Techniques Used: Expressing

    7) Product Images from "Optogenetic approaches addressing extracellular modulation of neural excitability"

    Article Title: Optogenetic approaches addressing extracellular modulation of neural excitability

    Journal: Scientific Reports

    doi: 10.1038/srep23947

    The contribution of acid-sensing ion channels to the bystander effect. ( a , b ) ASIC1 ( a ) and ASIC2a ( b ) expression in CA1. ( c ) Change in membrane resistance during amiloride (ASIC antagonist) application (lilac-shaded region) (n = 6–18 cells, 9 mice, two-way ANOVA for interaction between time and drug treatment (F (12,314) = 2.617, p = 0.0018, asterisks indicate significant time points after Dunnet’s multiple comparison test). Grey data points: control experiment in which no amiloride was applied (n = 4–16, 6 mice) (no significant change from baseline). ( d ) Change in bystander current during amiloride application (F (12,314) = 2.473, p = 0.0032, asterisks indicate significant time points). No significant change from baseline for untreated control cells. ( e ) Examples bystander current at baseline baseline (dark red), after 20 mins amiloride (pink), and after 30 mins of washout (lilac). ( f ) Example ChR2 and eArch3.0 bystander currents in 500 μM acetazolamide (pale traces indicate baseline recordings, dark traces indicate 15 mins acetazolamide exposure). ( g ) Change in bystander current magnitude after acetazolamide application (thick line represents group mean) (ChR2, n = 7 cells, 3 mice, paired t-test: t = 1.313, df = 6, p = 0.2372; eArch3.0, n = 7 cells, 3 mice, paired t-test: t = 6.177, df = 6, p = 0.0008). ( h ) Occasionally, acetazolamide caused eArch3.0 bystander neurons to exhibit inward ASIC-like currents during green light. ( i ) Extracellular pH measurements in CA1 (contralateral to site of opsin injection) in acute slices using a solid state metal wire oxide pH sensor (100 μm diameter, 5X magnification). ( j ) pH change in response to three minutes of light stimulation (470 nm for ChR2 and YFP-controls, 560 nm for eArch3.0). For ChR2, n = 24 recording sites, 13 slices, 3 animals. For eArch3.0, n = 21 recording sites, 11 slices, 3 animals. For YFP controls, n = 22 recording sites, 12 slices, 2 animals. ( k ) Zoom-in of last minute of baseline pH recording and first two minutes of light stimulation.
    Figure Legend Snippet: The contribution of acid-sensing ion channels to the bystander effect. ( a , b ) ASIC1 ( a ) and ASIC2a ( b ) expression in CA1. ( c ) Change in membrane resistance during amiloride (ASIC antagonist) application (lilac-shaded region) (n = 6–18 cells, 9 mice, two-way ANOVA for interaction between time and drug treatment (F (12,314) = 2.617, p = 0.0018, asterisks indicate significant time points after Dunnet’s multiple comparison test). Grey data points: control experiment in which no amiloride was applied (n = 4–16, 6 mice) (no significant change from baseline). ( d ) Change in bystander current during amiloride application (F (12,314) = 2.473, p = 0.0032, asterisks indicate significant time points). No significant change from baseline for untreated control cells. ( e ) Examples bystander current at baseline baseline (dark red), after 20 mins amiloride (pink), and after 30 mins of washout (lilac). ( f ) Example ChR2 and eArch3.0 bystander currents in 500 μM acetazolamide (pale traces indicate baseline recordings, dark traces indicate 15 mins acetazolamide exposure). ( g ) Change in bystander current magnitude after acetazolamide application (thick line represents group mean) (ChR2, n = 7 cells, 3 mice, paired t-test: t = 1.313, df = 6, p = 0.2372; eArch3.0, n = 7 cells, 3 mice, paired t-test: t = 6.177, df = 6, p = 0.0008). ( h ) Occasionally, acetazolamide caused eArch3.0 bystander neurons to exhibit inward ASIC-like currents during green light. ( i ) Extracellular pH measurements in CA1 (contralateral to site of opsin injection) in acute slices using a solid state metal wire oxide pH sensor (100 μm diameter, 5X magnification). ( j ) pH change in response to three minutes of light stimulation (470 nm for ChR2 and YFP-controls, 560 nm for eArch3.0). For ChR2, n = 24 recording sites, 13 slices, 3 animals. For eArch3.0, n = 21 recording sites, 11 slices, 3 animals. For YFP controls, n = 22 recording sites, 12 slices, 2 animals. ( k ) Zoom-in of last minute of baseline pH recording and first two minutes of light stimulation.

    Techniques Used: Expressing, Mouse Assay, Injection

    8) Product Images from "Down-regulation of ASICs current and the calcium transients by disrupting PICK1 protects primary cultured mouse cortical neurons from OGD-Rep insults"

    Article Title: Down-regulation of ASICs current and the calcium transients by disrupting PICK1 protects primary cultured mouse cortical neurons from OGD-Rep insults

    Journal: International Journal of Clinical and Experimental Pathology

    doi:

    ASIC1 and ASIC2a protein expression during OGD-Rep in three types of neurons. Cells were treated with OGD-Rep and prepared for immunofluorescence and western blot analysis. A, C. Representative pictures and summary data showing expressions of ASIC1 and ASIC2a using immunofluorescence. B, D. Representative bands and summary data of ASIC1 and ASIC2a expression in western blotting. Data are the mean ± SD in each group. *P
    Figure Legend Snippet: ASIC1 and ASIC2a protein expression during OGD-Rep in three types of neurons. Cells were treated with OGD-Rep and prepared for immunofluorescence and western blot analysis. A, C. Representative pictures and summary data showing expressions of ASIC1 and ASIC2a using immunofluorescence. B, D. Representative bands and summary data of ASIC1 and ASIC2a expression in western blotting. Data are the mean ± SD in each group. *P

    Techniques Used: Expressing, Immunofluorescence, Western Blot

    9) Product Images from "Upregulation of Spinal ASIC1 and NKCC1 Expression Contributes to Chronic Visceral Pain in Rats"

    Article Title: Upregulation of Spinal ASIC1 and NKCC1 Expression Contributes to Chronic Visceral Pain in Rats

    Journal: Frontiers in Molecular Neuroscience

    doi: 10.3389/fnmol.2020.611179

    Interaction of ASIC1 and NKCC1. (A) ASIC1 and NKCC1 were co-located in the spinal dorsal horn neurons. (B) Expression of NKCC1 in the spinal dorsal horn after intrathecal injection of Amiloride in NMD rats (* P
    Figure Legend Snippet: Interaction of ASIC1 and NKCC1. (A) ASIC1 and NKCC1 were co-located in the spinal dorsal horn neurons. (B) Expression of NKCC1 in the spinal dorsal horn after intrathecal injection of Amiloride in NMD rats (* P

    Techniques Used: Expressing, Injection

    NMD enhanced acid-sensing ion channel 1 (ASIC1) expression. (A) Expression of ASIC1 in the spinal dorsal horn of T13-L2 in NMD rats compared with CON rats (* P
    Figure Legend Snippet: NMD enhanced acid-sensing ion channel 1 (ASIC1) expression. (A) Expression of ASIC1 in the spinal dorsal horn of T13-L2 in NMD rats compared with CON rats (* P

    Techniques Used: Expressing

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    Alomone Labs anti asic1
    <t>ASIC1</t> and ASIC2a protein expression during OGD-Rep in three types of neurons. Cells were treated with OGD-Rep and prepared for immunofluorescence and western blot analysis. A, C. Representative pictures and summary data showing expressions of ASIC1 and ASIC2a using immunofluorescence. B, D. Representative bands and summary data of ASIC1 and ASIC2a expression in western blotting. Data are the mean ± SD in each group. *P
    Anti Asic1, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti asic1/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti asic1 - by Bioz Stars, 2022-08
    94/100 stars
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    ASIC1 and ASIC2a protein expression during OGD-Rep in three types of neurons. Cells were treated with OGD-Rep and prepared for immunofluorescence and western blot analysis. A, C. Representative pictures and summary data showing expressions of ASIC1 and ASIC2a using immunofluorescence. B, D. Representative bands and summary data of ASIC1 and ASIC2a expression in western blotting. Data are the mean ± SD in each group. *P

    Journal: International Journal of Clinical and Experimental Pathology

    Article Title: Down-regulation of ASICs current and the calcium transients by disrupting PICK1 protects primary cultured mouse cortical neurons from OGD-Rep insults

    doi:

    Figure Lengend Snippet: ASIC1 and ASIC2a protein expression during OGD-Rep in three types of neurons. Cells were treated with OGD-Rep and prepared for immunofluorescence and western blot analysis. A, C. Representative pictures and summary data showing expressions of ASIC1 and ASIC2a using immunofluorescence. B, D. Representative bands and summary data of ASIC1 and ASIC2a expression in western blotting. Data are the mean ± SD in each group. *P

    Article Snippet: Cerebral cortical neurons were collected and fixed with 4% paraformaldehyde in PBS for 30 min. Then permeabilized with PBS/0.3% Triton X-100 for 30 min, and all cells were blocked with 2% goat serum and 1% bovine serum albumin (BSA) in PBS for 1 h, and then incubated with anti-ASIC1 (Alomone labs, Israel) or anti-ASIC2a (Alomone labs, Israel) overnight at 4°C.

    Techniques: Expressing, Immunofluorescence, Western Blot

    ASIC1, involved in the enterodynia of PMS offspring rats, was co‐localization with spinal dorsal horn neurons. (A) PMS significantly reduced the pain threshold of the offspring rats at the age of 6 weeks compared with CON rats ( n = 7 for each group, *** P = 0.0006

    Journal: CNS Neuroscience & Therapeutics

    Article Title: Upregulation of spinal ASIC1 by miR‐485 mediates enterodynia in adult offspring rats with prenatal maternal stress, et al. Upregulation of spinal ASIC1 by miR‐485 mediates enterodynia in adult offspring rats with prenatal maternal stress

    doi: 10.1111/cns.13542

    Figure Lengend Snippet: ASIC1, involved in the enterodynia of PMS offspring rats, was co‐localization with spinal dorsal horn neurons. (A) PMS significantly reduced the pain threshold of the offspring rats at the age of 6 weeks compared with CON rats ( n = 7 for each group, *** P = 0.0006

    Article Snippet: The primary antibodies incubated on the spinal cord included anti‐ASIC1 (1:50, Alomone Labs, Jerusalem, Israel), anti‐NeuN (1:50, Merck Millipore, Darmstadt, Germany), anti‐GFAP (1:100, Cell Signaling Technology, Danvers, MA, USA), and anti‐CD11b (1:50, Bio‐Rad, California, USA).

    Techniques:

    MiR‐485 agomir negatively regulated ASIC1 expression and reversed the synaptic transmission of spinal dorsal horn in PMS offspring rats. (A) The expression of ASIC1 was obviously reduced in spinal dorsal horn after intrathecally injecting miR‐485 agomir for consecutive 7 days ( n = 7 for each group, * P

    Journal: CNS Neuroscience & Therapeutics

    Article Title: Upregulation of spinal ASIC1 by miR‐485 mediates enterodynia in adult offspring rats with prenatal maternal stress, et al. Upregulation of spinal ASIC1 by miR‐485 mediates enterodynia in adult offspring rats with prenatal maternal stress

    doi: 10.1111/cns.13542

    Figure Lengend Snippet: MiR‐485 agomir negatively regulated ASIC1 expression and reversed the synaptic transmission of spinal dorsal horn in PMS offspring rats. (A) The expression of ASIC1 was obviously reduced in spinal dorsal horn after intrathecally injecting miR‐485 agomir for consecutive 7 days ( n = 7 for each group, * P

    Article Snippet: The primary antibodies incubated on the spinal cord included anti‐ASIC1 (1:50, Alomone Labs, Jerusalem, Israel), anti‐NeuN (1:50, Merck Millipore, Darmstadt, Germany), anti‐GFAP (1:100, Cell Signaling Technology, Danvers, MA, USA), and anti‐CD11b (1:50, Bio‐Rad, California, USA).

    Techniques: Expressing, Transmission Assay

    MiR‐485 was reduced in the spinal dorsal horn of PMS offspring rats and co‐expressed with ASIC1 in spinal dorsal horn. (A) PMS significantly reduced miR‐485 expression in T13‐L2 spinal dorsal horn at the age of 6 weeks compared with CON ( n = 4 for each group, ** P

    Journal: CNS Neuroscience & Therapeutics

    Article Title: Upregulation of spinal ASIC1 by miR‐485 mediates enterodynia in adult offspring rats with prenatal maternal stress, et al. Upregulation of spinal ASIC1 by miR‐485 mediates enterodynia in adult offspring rats with prenatal maternal stress

    doi: 10.1111/cns.13542

    Figure Lengend Snippet: MiR‐485 was reduced in the spinal dorsal horn of PMS offspring rats and co‐expressed with ASIC1 in spinal dorsal horn. (A) PMS significantly reduced miR‐485 expression in T13‐L2 spinal dorsal horn at the age of 6 weeks compared with CON ( n = 4 for each group, ** P

    Article Snippet: The primary antibodies incubated on the spinal cord included anti‐ASIC1 (1:50, Alomone Labs, Jerusalem, Israel), anti‐NeuN (1:50, Merck Millipore, Darmstadt, Germany), anti‐GFAP (1:100, Cell Signaling Technology, Danvers, MA, USA), and anti‐CD11b (1:50, Bio‐Rad, California, USA).

    Techniques: Expressing

    Expressions of ASIC subunits in rat cardiomyocytes. ( a ) Expressions of ASIC1, 2a and 3 proteins in rat cultured cardiomyocytes. Individual ASIC subunits peptides were added as negative controls. The blots were cropped from Supplementary Fig. S1a . The representative full-length blot for ASIC2a was shown in Supplementary Fig. S2 . ( b ) Expressions of ASIC1, 2 and 3 transcripts in cultured rat ventricular myocytes. GAPDH transcript was used as control. M: marker; 1, 4: cardiomyocytes; 2: cortex; 3: negative control. ( c ) Double - labeling fluorescence of ASICs ( green ) and nucleus ( blue , marker: Hoechst 33258) in cultured ratcardiomyocytes. NC: pretreatment with immunogenic peptide as negative control. Scale bars: 20 μm. All data were represented from at least three similar independent experiments.

    Journal: Scientific Reports

    Article Title: Multiple H+ sensors mediate the extracellular acidification-induced [Ca2+]i elevation in cultured rat ventricular cardiomyocytes

    doi: 10.1038/srep44951

    Figure Lengend Snippet: Expressions of ASIC subunits in rat cardiomyocytes. ( a ) Expressions of ASIC1, 2a and 3 proteins in rat cultured cardiomyocytes. Individual ASIC subunits peptides were added as negative controls. The blots were cropped from Supplementary Fig. S1a . The representative full-length blot for ASIC2a was shown in Supplementary Fig. S2 . ( b ) Expressions of ASIC1, 2 and 3 transcripts in cultured rat ventricular myocytes. GAPDH transcript was used as control. M: marker; 1, 4: cardiomyocytes; 2: cortex; 3: negative control. ( c ) Double - labeling fluorescence of ASICs ( green ) and nucleus ( blue , marker: Hoechst 33258) in cultured ratcardiomyocytes. NC: pretreatment with immunogenic peptide as negative control. Scale bars: 20 μm. All data were represented from at least three similar independent experiments.

    Article Snippet: After blocking, the proteins were probed with the appropriate primary antibodies against ASIC1, ASIC2a, ASIC3, TRPV1 (Alomone labs, Jerusalem, Israel.ASC-014, ASC-012, ASC-018, ACC-030, all in 1:200 dilution) and OGR1 (Santa Cruz Biotechnology, USA. sc-98437).

    Techniques: Cell Culture, Marker, Negative Control, Labeling, Fluorescence