anti aqp5 rabbit polyclonal antibody  (Alomone Labs)


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    Alomone Labs anti aqp5 rabbit polyclonal antibody
    Impact of KvLQT1 activation on alveolar type I (AT1) and II (ATII) epithelial cell markers in thiourea-challenged WT mice. Representative immunofluorescence images (left panels) of lung sections (5 μm, Scale: 100 μm) from WT control mice (PBS/PBS), and WT mice challenged with thiourea (TU, 5 mg/kg i.p., PBS/TU) and treated or not (PBS) with the KvLQT1 activator R-L3 (R-L3/TU) stained with the ATI markers podoplanin (A) , n = 50 images, <t>AQP5</t> (B) , n = 59–60 or ATII marker pro-SPC (C) , n = 48–51). Nuclei were stained by DAPI. Quantification (right panels) of podoplanin, AQP5, and pro-SPC marker intensities was made with a protocol exploited by ICY software. Values are presented as means ± SEM. Non-parametric Mann-Whitney t-test (Agostino/Pearson normality test: negative) for panel (A) . One-way ANOVA non-parametric comparison test (normality Agostino/Pearson test: negative) for panel (B , C) . * p < .05, ** p < .01 or **** p < .0001 vs . PBS/PBS, ## p < .01 vs . PBS/TU.
    Anti Aqp5 Rabbit Polyclonal Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti aqp5 rabbit polyclonal antibody/product/Alomone Labs
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti aqp5 rabbit polyclonal antibody - by Bioz Stars, 2024-06
    95/100 stars

    Images

    1) Product Images from "Impact of KvLQT1 potassium channel modulation on alveolar fluid homeostasis in an animal model of thiourea-induced lung edema"

    Article Title: Impact of KvLQT1 potassium channel modulation on alveolar fluid homeostasis in an animal model of thiourea-induced lung edema

    Journal: Frontiers in Physiology

    doi: 10.3389/fphys.2022.1069466

    Impact of KvLQT1 activation on alveolar type I (AT1) and II (ATII) epithelial cell markers in thiourea-challenged WT mice. Representative immunofluorescence images (left panels) of lung sections (5 μm, Scale: 100 μm) from WT control mice (PBS/PBS), and WT mice challenged with thiourea (TU, 5 mg/kg i.p., PBS/TU) and treated or not (PBS) with the KvLQT1 activator R-L3 (R-L3/TU) stained with the ATI markers podoplanin (A) , n = 50 images, AQP5 (B) , n = 59–60 or ATII marker pro-SPC (C) , n = 48–51). Nuclei were stained by DAPI. Quantification (right panels) of podoplanin, AQP5, and pro-SPC marker intensities was made with a protocol exploited by ICY software. Values are presented as means ± SEM. Non-parametric Mann-Whitney t-test (Agostino/Pearson normality test: negative) for panel (A) . One-way ANOVA non-parametric comparison test (normality Agostino/Pearson test: negative) for panel (B , C) . * p < .05, ** p < .01 or **** p < .0001 vs . PBS/PBS, ## p < .01 vs . PBS/TU.
    Figure Legend Snippet: Impact of KvLQT1 activation on alveolar type I (AT1) and II (ATII) epithelial cell markers in thiourea-challenged WT mice. Representative immunofluorescence images (left panels) of lung sections (5 μm, Scale: 100 μm) from WT control mice (PBS/PBS), and WT mice challenged with thiourea (TU, 5 mg/kg i.p., PBS/TU) and treated or not (PBS) with the KvLQT1 activator R-L3 (R-L3/TU) stained with the ATI markers podoplanin (A) , n = 50 images, AQP5 (B) , n = 59–60 or ATII marker pro-SPC (C) , n = 48–51). Nuclei were stained by DAPI. Quantification (right panels) of podoplanin, AQP5, and pro-SPC marker intensities was made with a protocol exploited by ICY software. Values are presented as means ± SEM. Non-parametric Mann-Whitney t-test (Agostino/Pearson normality test: negative) for panel (A) . One-way ANOVA non-parametric comparison test (normality Agostino/Pearson test: negative) for panel (B , C) . * p < .05, ** p < .01 or **** p < .0001 vs . PBS/PBS, ## p < .01 vs . PBS/TU.

    Techniques Used: Activation Assay, Immunofluorescence, Staining, Marker, Software, MANN-WHITNEY

    rabbit anti aqp5 polyclonal antibody  (Alomone Labs)


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    Alomone Labs rabbit anti aqp5 polyclonal antibody
    Rabbit Anti Aqp5 Polyclonal Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti aqp5 polyclonal antibody/product/Alomone Labs
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti aqp5 polyclonal antibody - by Bioz Stars, 2024-06
    86/100 stars

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    anti aqp5 rabbit polyclonal antibody  (Alomone Labs)


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    Alomone Labs anti aqp5 rabbit polyclonal antibody
    Impact of KvLQT1 activation on alveolar type I (AT1) and II (ATII) epithelial cell markers in thiourea-challenged WT mice. Representative immunofluorescence images (left panels) of lung sections (5 μm, Scale: 100 μm) from WT control mice (PBS/PBS), and WT mice challenged with thiourea (TU, 5 mg/kg i.p., PBS/TU) and treated or not (PBS) with the KvLQT1 activator R-L3 (R-L3/TU) stained with the ATI markers podoplanin (A) , n = 50 images, <t>AQP5</t> (B) , n = 59–60 or ATII marker pro-SPC (C) , n = 48–51). Nuclei were stained by DAPI. Quantification (right panels) of podoplanin, AQP5, and pro-SPC marker intensities was made with a protocol exploited by ICY software. Values are presented as means ± SEM. Non-parametric Mann-Whitney t-test (Agostino/Pearson normality test: negative) for panel (A) . One-way ANOVA non-parametric comparison test (normality Agostino/Pearson test: negative) for panel (B , C) . * p < .05, ** p < .01 or **** p < .0001 vs . PBS/PBS, ## p < .01 vs . PBS/TU.
    Anti Aqp5 Rabbit Polyclonal Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti aqp5 rabbit polyclonal antibody/product/Alomone Labs
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti aqp5 rabbit polyclonal antibody - by Bioz Stars, 2024-06
    95/100 stars

    Images

    1) Product Images from "Impact of KvLQT1 potassium channel modulation on alveolar fluid homeostasis in an animal model of thiourea-induced lung edema"

    Article Title: Impact of KvLQT1 potassium channel modulation on alveolar fluid homeostasis in an animal model of thiourea-induced lung edema

    Journal: Frontiers in Physiology

    doi: 10.3389/fphys.2022.1069466

    Impact of KvLQT1 activation on alveolar type I (AT1) and II (ATII) epithelial cell markers in thiourea-challenged WT mice. Representative immunofluorescence images (left panels) of lung sections (5 μm, Scale: 100 μm) from WT control mice (PBS/PBS), and WT mice challenged with thiourea (TU, 5 mg/kg i.p., PBS/TU) and treated or not (PBS) with the KvLQT1 activator R-L3 (R-L3/TU) stained with the ATI markers podoplanin (A) , n = 50 images, AQP5 (B) , n = 59–60 or ATII marker pro-SPC (C) , n = 48–51). Nuclei were stained by DAPI. Quantification (right panels) of podoplanin, AQP5, and pro-SPC marker intensities was made with a protocol exploited by ICY software. Values are presented as means ± SEM. Non-parametric Mann-Whitney t-test (Agostino/Pearson normality test: negative) for panel (A) . One-way ANOVA non-parametric comparison test (normality Agostino/Pearson test: negative) for panel (B , C) . * p < .05, ** p < .01 or **** p < .0001 vs . PBS/PBS, ## p < .01 vs . PBS/TU.
    Figure Legend Snippet: Impact of KvLQT1 activation on alveolar type I (AT1) and II (ATII) epithelial cell markers in thiourea-challenged WT mice. Representative immunofluorescence images (left panels) of lung sections (5 μm, Scale: 100 μm) from WT control mice (PBS/PBS), and WT mice challenged with thiourea (TU, 5 mg/kg i.p., PBS/TU) and treated or not (PBS) with the KvLQT1 activator R-L3 (R-L3/TU) stained with the ATI markers podoplanin (A) , n = 50 images, AQP5 (B) , n = 59–60 or ATII marker pro-SPC (C) , n = 48–51). Nuclei were stained by DAPI. Quantification (right panels) of podoplanin, AQP5, and pro-SPC marker intensities was made with a protocol exploited by ICY software. Values are presented as means ± SEM. Non-parametric Mann-Whitney t-test (Agostino/Pearson normality test: negative) for panel (A) . One-way ANOVA non-parametric comparison test (normality Agostino/Pearson test: negative) for panel (B , C) . * p < .05, ** p < .01 or **** p < .0001 vs . PBS/PBS, ## p < .01 vs . PBS/TU.

    Techniques Used: Activation Assay, Immunofluorescence, Staining, Marker, Software, MANN-WHITNEY

    anti aqp5 rabbit polyclonal antibody  (Alomone Labs)


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    Alomone Labs anti aqp5 rabbit polyclonal antibody
    Anti Aqp5 Rabbit Polyclonal Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti aqp5 rabbit polyclonal antibody/product/Alomone Labs
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti aqp5 rabbit polyclonal antibody - by Bioz Stars, 2024-06
    86/100 stars

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    rabbit polyclonal nti aquaporin 5 antibody  (Alomone Labs)


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    Alomone Labs rabbit polyclonal nti aquaporin 5 antibody
    Rabbit Polyclonal Nti Aquaporin 5 Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit polyclonal nti aquaporin 5 antibody/product/Alomone Labs
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit polyclonal nti aquaporin 5 antibody - by Bioz Stars, 2024-06
    95/100 stars

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    rabbit polyclonal anti aqp5 antibody  (Alomone Labs)


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    Alomone Labs rabbit polyclonal anti aqp5 antibody
    Spatiotemporal expression of CXC-chemokine receptor 4 (CXCR4) and its ligand, CXCL12, and developmental genes in embryonic organs. ( A ) Schematic diagram of embryonic submandibular gland (eSMG) isolation and ex vivo culture. ( B ) Ex vivo branching morphogenesis of eSMGs from embryonic day (E) 13 to 17, showing epithelial growth and retraction of mesenchyme. Scale bars: 500 µm. ( C ) Temporal mRNA expression patterns of keratin 7 ( Krt7 ), <t>aquaporin</t> <t>5</t> ( <t>Aqp5</t> ), e-cadherin ( Cdh1 ), Krt15 , Cxcr4 , and Cxcl12 were measured from E13 to E17 by qPCR ( n = 3). ( D ) Epithelial (Epi) and mesenchymal (Mes) expression of Cxcr4 , Cxcl12 , odd-skipped related transcription factor 1 ( Osr1 ), and Cdh1 were quantified by qPCR at E13. The comparative C t values are expressed as fold increase relative to the epithelium ( n = 3). ( E ) Representative images showing expression of CXCR4 and CXCL12 in eSMG (upper) and their colocalization (lower) ( n = 3, scale bar: 500 µm). ( F ) Representative immunofluorescence images of CXCR4 and CXCL12 expression in E12 embryonic lung and pancreas ( n = 4); whole view (left two panels; scale bar: 500 µm) and magnified lumen structures (right two panels; scale bar: 50 µm). Data are presented as the mean ± SEM; * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, NS: not significant; two-way ANOVA ( C ); t -test ( D ).
    Rabbit Polyclonal Anti Aqp5 Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit polyclonal anti aqp5 antibody/product/Alomone Labs
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit polyclonal anti aqp5 antibody - by Bioz Stars, 2024-06
    95/100 stars

    Images

    1) Product Images from "CXCR4 Regulates Temporal Differentiation via PRC1 Complex in Organogenesis of Epithelial Glands"

    Article Title: CXCR4 Regulates Temporal Differentiation via PRC1 Complex in Organogenesis of Epithelial Glands

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms22020619

    Spatiotemporal expression of CXC-chemokine receptor 4 (CXCR4) and its ligand, CXCL12, and developmental genes in embryonic organs. ( A ) Schematic diagram of embryonic submandibular gland (eSMG) isolation and ex vivo culture. ( B ) Ex vivo branching morphogenesis of eSMGs from embryonic day (E) 13 to 17, showing epithelial growth and retraction of mesenchyme. Scale bars: 500 µm. ( C ) Temporal mRNA expression patterns of keratin 7 ( Krt7 ), aquaporin 5 ( Aqp5 ), e-cadherin ( Cdh1 ), Krt15 , Cxcr4 , and Cxcl12 were measured from E13 to E17 by qPCR ( n = 3). ( D ) Epithelial (Epi) and mesenchymal (Mes) expression of Cxcr4 , Cxcl12 , odd-skipped related transcription factor 1 ( Osr1 ), and Cdh1 were quantified by qPCR at E13. The comparative C t values are expressed as fold increase relative to the epithelium ( n = 3). ( E ) Representative images showing expression of CXCR4 and CXCL12 in eSMG (upper) and their colocalization (lower) ( n = 3, scale bar: 500 µm). ( F ) Representative immunofluorescence images of CXCR4 and CXCL12 expression in E12 embryonic lung and pancreas ( n = 4); whole view (left two panels; scale bar: 500 µm) and magnified lumen structures (right two panels; scale bar: 50 µm). Data are presented as the mean ± SEM; * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, NS: not significant; two-way ANOVA ( C ); t -test ( D ).
    Figure Legend Snippet: Spatiotemporal expression of CXC-chemokine receptor 4 (CXCR4) and its ligand, CXCL12, and developmental genes in embryonic organs. ( A ) Schematic diagram of embryonic submandibular gland (eSMG) isolation and ex vivo culture. ( B ) Ex vivo branching morphogenesis of eSMGs from embryonic day (E) 13 to 17, showing epithelial growth and retraction of mesenchyme. Scale bars: 500 µm. ( C ) Temporal mRNA expression patterns of keratin 7 ( Krt7 ), aquaporin 5 ( Aqp5 ), e-cadherin ( Cdh1 ), Krt15 , Cxcr4 , and Cxcl12 were measured from E13 to E17 by qPCR ( n = 3). ( D ) Epithelial (Epi) and mesenchymal (Mes) expression of Cxcr4 , Cxcl12 , odd-skipped related transcription factor 1 ( Osr1 ), and Cdh1 were quantified by qPCR at E13. The comparative C t values are expressed as fold increase relative to the epithelium ( n = 3). ( E ) Representative images showing expression of CXCR4 and CXCL12 in eSMG (upper) and their colocalization (lower) ( n = 3, scale bar: 500 µm). ( F ) Representative immunofluorescence images of CXCR4 and CXCL12 expression in E12 embryonic lung and pancreas ( n = 4); whole view (left two panels; scale bar: 500 µm) and magnified lumen structures (right two panels; scale bar: 50 µm). Data are presented as the mean ± SEM; * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, NS: not significant; two-way ANOVA ( C ); t -test ( D ).

    Techniques Used: Expressing, Isolation, Ex Vivo, Immunofluorescence

    AMD3100-induced precocious differentiation of epithelial cells. ( A , B ) Representative contour tracing ( A ) and bud number changes ( B ) of control and AMD3100-treated eSMGs during 48 h at 6-h intervals ( n = 3). ( C ) EdU staining results at 6 and 24 h after AMD3100 treatment. EdU in green and PNA in gray ( n = 4, scale bar: 500 µm). ( D ) Immunostaining results of Ki67 (red) and F-actin (green) in acini and duct of eSMGs 24 h after AMD3100 treatment. Morphologies of acinar buds and duct cells are outlined with white dotted lines. Scale bar: 50 µm. ( E ) Duct widths of control and AMD3100-treated eSMGs were visualized via F-actin-based intensity profiles of horizontal sectioning of ducts 24 h after the treatment. ( F ) Duct widths of control and AMD3100-treated eSMGs were quantified 24 h after the treatment ( n = 9). ( G ) Immunostaining results of AQP5 (green) and KRT7 (red). Magnified regions of acinar buds are marked with white dotted squares. The white arrows (middle panels) indicate areas with the highest AQP5 expression ( n = 4, scale bar: left, 100 µm; middle and right, 50 µm). Data are presented as the mean ± SEM; * p < 0.05, **** p < 0.0001; t -test.
    Figure Legend Snippet: AMD3100-induced precocious differentiation of epithelial cells. ( A , B ) Representative contour tracing ( A ) and bud number changes ( B ) of control and AMD3100-treated eSMGs during 48 h at 6-h intervals ( n = 3). ( C ) EdU staining results at 6 and 24 h after AMD3100 treatment. EdU in green and PNA in gray ( n = 4, scale bar: 500 µm). ( D ) Immunostaining results of Ki67 (red) and F-actin (green) in acini and duct of eSMGs 24 h after AMD3100 treatment. Morphologies of acinar buds and duct cells are outlined with white dotted lines. Scale bar: 50 µm. ( E ) Duct widths of control and AMD3100-treated eSMGs were visualized via F-actin-based intensity profiles of horizontal sectioning of ducts 24 h after the treatment. ( F ) Duct widths of control and AMD3100-treated eSMGs were quantified 24 h after the treatment ( n = 9). ( G ) Immunostaining results of AQP5 (green) and KRT7 (red). Magnified regions of acinar buds are marked with white dotted squares. The white arrows (middle panels) indicate areas with the highest AQP5 expression ( n = 4, scale bar: left, 100 µm; middle and right, 50 µm). Data are presented as the mean ± SEM; * p < 0.05, **** p < 0.0001; t -test.

    Techniques Used: Staining, Immunostaining, Expressing

    eSMGs lacking PRC1 develop precocious differentiation in acini and ducts. ( A ) Schematic diagram of AMD3100-induced effect on organogenesis. Down-regulation of PRC1 genes leads to the inability of methylation site recognition by chromobox 8 (CBX8), a polycomb group protein ( 1 ), resulting in failure of the ubiquitin-ligating activity of ring finger protein 1 (RING1) ( 2 ) and thus, disabled DNA condensation ( 3 ). Hence, the developmental genes are readily transcribed by RNA polymerase II. ( B ) Representative images showing ubiquitin expression at Lys119 of histone 2A (H2AK119ub) in control and AMD3100-treated eSMGs 24 h after the treatment ( n = 4, scale bar: 50 µm). ( C ) Mean fluorescence intensities of H2AK119ub in epithelial buds per z -stack of 0.91 µm (25 stacks per end bud). False discovery rate (FDR) q -values of the differences in H2AK119ub expression at each of the 25 stacks are shown in the heat map, in which 23 out of 25 stacks marked a significant difference between control and AMD3100-treated eSMGs. -log( q -value) > 1.3 is considered significant ( q < 0.05; t -test with 5% FDR correction). The heat map (right) shows the color profile ( n = 4). ( D ) Methyl-seq results from control and AMD3100-treated eSMGs. Ten eSMGs per group were homogenized and analyzed. ( E ) Relative DAPI intensities of end buds in control, AMD3100-, and UNC3866-treated groups are expressed as a colored heat map (0–65,535 gray-value). Magnified regions are marked with white dotted squares ( n = 4, scale bar: 20 µm). ( F ) Quantification of DAPI intensities in nuclei of control (CON), AMD3100 (AMD)-, and UNC3866 (UNC)-treated eSMGs ( n = 18). ( G ) Immunostaining results of AQP5 (yellow) and KRT7 (gray) in eSMGs treated with AMD3100 or UNC3866. eSMGs were fixed and immunostained 24 h after the treatment ( n = 4, scale bar: upper panel, 50 µm; lower panel, 500 µm). ( H ) Quantification of AQP5 intensities in acinar buds of eSMGs treated with AMD3100 (AMD) or UNC3866 (UNC) ( n = 30). ( I ) Quantification of KRT7 intensities in ducts of eSMGs treated with AMD3100 (AMD) or UNC3866 (UNC) ( n = 4). Data are presented as the mean ± SEM; ** p < 0.01, *** p < 0.001, **** p < 0.0001; one-way ANOVA.
    Figure Legend Snippet: eSMGs lacking PRC1 develop precocious differentiation in acini and ducts. ( A ) Schematic diagram of AMD3100-induced effect on organogenesis. Down-regulation of PRC1 genes leads to the inability of methylation site recognition by chromobox 8 (CBX8), a polycomb group protein ( 1 ), resulting in failure of the ubiquitin-ligating activity of ring finger protein 1 (RING1) ( 2 ) and thus, disabled DNA condensation ( 3 ). Hence, the developmental genes are readily transcribed by RNA polymerase II. ( B ) Representative images showing ubiquitin expression at Lys119 of histone 2A (H2AK119ub) in control and AMD3100-treated eSMGs 24 h after the treatment ( n = 4, scale bar: 50 µm). ( C ) Mean fluorescence intensities of H2AK119ub in epithelial buds per z -stack of 0.91 µm (25 stacks per end bud). False discovery rate (FDR) q -values of the differences in H2AK119ub expression at each of the 25 stacks are shown in the heat map, in which 23 out of 25 stacks marked a significant difference between control and AMD3100-treated eSMGs. -log( q -value) > 1.3 is considered significant ( q < 0.05; t -test with 5% FDR correction). The heat map (right) shows the color profile ( n = 4). ( D ) Methyl-seq results from control and AMD3100-treated eSMGs. Ten eSMGs per group were homogenized and analyzed. ( E ) Relative DAPI intensities of end buds in control, AMD3100-, and UNC3866-treated groups are expressed as a colored heat map (0–65,535 gray-value). Magnified regions are marked with white dotted squares ( n = 4, scale bar: 20 µm). ( F ) Quantification of DAPI intensities in nuclei of control (CON), AMD3100 (AMD)-, and UNC3866 (UNC)-treated eSMGs ( n = 18). ( G ) Immunostaining results of AQP5 (yellow) and KRT7 (gray) in eSMGs treated with AMD3100 or UNC3866. eSMGs were fixed and immunostained 24 h after the treatment ( n = 4, scale bar: upper panel, 50 µm; lower panel, 500 µm). ( H ) Quantification of AQP5 intensities in acinar buds of eSMGs treated with AMD3100 (AMD) or UNC3866 (UNC) ( n = 30). ( I ) Quantification of KRT7 intensities in ducts of eSMGs treated with AMD3100 (AMD) or UNC3866 (UNC) ( n = 4). Data are presented as the mean ± SEM; ** p < 0.01, *** p < 0.001, **** p < 0.0001; one-way ANOVA.

    Techniques Used: Methylation, Activity Assay, Expressing, Fluorescence, Immunostaining

    rabbit polyclonal 8 anti aqp5  (Alomone Labs)


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    Alomone Labs rabbit polyclonal 8 anti aqp5
    Rabbit Polyclonal 8 Anti Aqp5, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit polyclonal 8 anti aqp5/product/Alomone Labs
    Average 95 stars, based on 1 article reviews
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    95/100 stars

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    rabbit polyclonal antibodies against aquaporin 5  (Alomone Labs)


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    Alomone Labs rabbit polyclonal antibodies against aquaporin 5
    Histological analysis of salivary glands. ( A ) Representative histological images of PAS and MT staining. More mucin-containing acini and less periductal fibrosis were observed in the RI+KGF-1 group than in the RI group. ( B ) Representative immunohistochemical images showing HIF1a, salivary epithelial cells <t>(AQP5</t> stained) and endothelial cells (CD31 stained). The expression of HIF1a was higher and the expressions of AQP5 and CD31 were lower in the RI group than in treatment naïve controls. HIF1a staining intensities was lesser, and AQP5 and CD31 staining intensities were greater in the RI+KGF-1 group than in the RI group. (N; treatment naïve control group, RI; RI group, KGF; RI+KGF-1 group). Results are presented as means ± SDs; *, vs. treatment naïve controls; # , vs. the RI group. * p < 0.05, # p < 0.05 ( n = 6 mice per group) (N; treatment naïve controls, RI; RI group, KGF; RI+KGF-1 group), Scale bar: 50 μm. ( C ) More mucin-containing acini and less periductal fibrosis were observed in the RI+KGF-1 group than in the RI group. The expression of HIF1a was higher and the expressions of AQP5 and CD31 were lower in the RI group than in treatment naïve controls. HIF1a staining intensities was lesser, and AQP5 and CD31 staining intensities were greater in the RI+KGF-1 group than in the RI group. (N; treatment naïve control group, RI; RI group, KGF; RI+KGF-1 group). Results are presented as means ± SDs; *, vs. treatment naïve controls; # , vs. the RI group. * p < 0.05, # p < 0.05 ( n = 6 mice per group) (N; treatment naïve controls, RI; RI group, KGF; RI+KGF-1 group), Scale bar: 50 μm.
    Rabbit Polyclonal Antibodies Against Aquaporin 5, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit polyclonal antibodies against aquaporin 5/product/Alomone Labs
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit polyclonal antibodies against aquaporin 5 - by Bioz Stars, 2024-06
    95/100 stars

    Images

    1) Product Images from "Keratinocyte Growth Factor-1 Protects Radioiodine-Induced Salivary Gland Dysfunction in Mice"

    Article Title: Keratinocyte Growth Factor-1 Protects Radioiodine-Induced Salivary Gland Dysfunction in Mice

    Journal: International Journal of Environmental Research and Public Health

    doi: 10.3390/ijerph17176322

    Histological analysis of salivary glands. ( A ) Representative histological images of PAS and MT staining. More mucin-containing acini and less periductal fibrosis were observed in the RI+KGF-1 group than in the RI group. ( B ) Representative immunohistochemical images showing HIF1a, salivary epithelial cells (AQP5 stained) and endothelial cells (CD31 stained). The expression of HIF1a was higher and the expressions of AQP5 and CD31 were lower in the RI group than in treatment naïve controls. HIF1a staining intensities was lesser, and AQP5 and CD31 staining intensities were greater in the RI+KGF-1 group than in the RI group. (N; treatment naïve control group, RI; RI group, KGF; RI+KGF-1 group). Results are presented as means ± SDs; *, vs. treatment naïve controls; # , vs. the RI group. * p < 0.05, # p < 0.05 ( n = 6 mice per group) (N; treatment naïve controls, RI; RI group, KGF; RI+KGF-1 group), Scale bar: 50 μm. ( C ) More mucin-containing acini and less periductal fibrosis were observed in the RI+KGF-1 group than in the RI group. The expression of HIF1a was higher and the expressions of AQP5 and CD31 were lower in the RI group than in treatment naïve controls. HIF1a staining intensities was lesser, and AQP5 and CD31 staining intensities were greater in the RI+KGF-1 group than in the RI group. (N; treatment naïve control group, RI; RI group, KGF; RI+KGF-1 group). Results are presented as means ± SDs; *, vs. treatment naïve controls; # , vs. the RI group. * p < 0.05, # p < 0.05 ( n = 6 mice per group) (N; treatment naïve controls, RI; RI group, KGF; RI+KGF-1 group), Scale bar: 50 μm.
    Figure Legend Snippet: Histological analysis of salivary glands. ( A ) Representative histological images of PAS and MT staining. More mucin-containing acini and less periductal fibrosis were observed in the RI+KGF-1 group than in the RI group. ( B ) Representative immunohistochemical images showing HIF1a, salivary epithelial cells (AQP5 stained) and endothelial cells (CD31 stained). The expression of HIF1a was higher and the expressions of AQP5 and CD31 were lower in the RI group than in treatment naïve controls. HIF1a staining intensities was lesser, and AQP5 and CD31 staining intensities were greater in the RI+KGF-1 group than in the RI group. (N; treatment naïve control group, RI; RI group, KGF; RI+KGF-1 group). Results are presented as means ± SDs; *, vs. treatment naïve controls; # , vs. the RI group. * p < 0.05, # p < 0.05 ( n = 6 mice per group) (N; treatment naïve controls, RI; RI group, KGF; RI+KGF-1 group), Scale bar: 50 μm. ( C ) More mucin-containing acini and less periductal fibrosis were observed in the RI+KGF-1 group than in the RI group. The expression of HIF1a was higher and the expressions of AQP5 and CD31 were lower in the RI group than in treatment naïve controls. HIF1a staining intensities was lesser, and AQP5 and CD31 staining intensities were greater in the RI+KGF-1 group than in the RI group. (N; treatment naïve control group, RI; RI group, KGF; RI+KGF-1 group). Results are presented as means ± SDs; *, vs. treatment naïve controls; # , vs. the RI group. * p < 0.05, # p < 0.05 ( n = 6 mice per group) (N; treatment naïve controls, RI; RI group, KGF; RI+KGF-1 group), Scale bar: 50 μm.

    Techniques Used: Staining, Immunohistochemical staining, Expressing

    rabbit polyclonal antibody anti aqp5  (Alomone Labs)


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    Alomone Labs rabbit polyclonal antibody anti aqp5
    Pig mandibular gland. <t>AQP5</t> binding sites at duct (*) and demilune (↑) level in CM, FP and CP groups.
    Rabbit Polyclonal Antibody Anti Aqp5, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "Influence of Different Feed Physical Forms on Mandibular Gland in Growing Pigs"

    Article Title: Influence of Different Feed Physical Forms on Mandibular Gland in Growing Pigs

    Journal: Animals : an Open Access Journal from MDPI

    doi: 10.3390/ani10050910

    Pig mandibular gland. AQP5 binding sites at duct (*) and demilune (↑) level in CM, FP and CP groups.
    Figure Legend Snippet: Pig mandibular gland. AQP5 binding sites at duct (*) and demilune (↑) level in CM, FP and CP groups.

    Techniques Used: Binding Assay

    rabbit polyclonal antibodies  (Alomone Labs)


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    Alomone Labs rabbit polyclonal antibodies
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    rabbit polyclonal anti aqp5  (Alomone Labs)


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    Alomone Labs rabbit polyclonal anti aqp5
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    Alomone Labs anti aqp5 rabbit polyclonal antibody
    Impact of KvLQT1 activation on alveolar type I (AT1) and II (ATII) epithelial cell markers in thiourea-challenged WT mice. Representative immunofluorescence images (left panels) of lung sections (5 μm, Scale: 100 μm) from WT control mice (PBS/PBS), and WT mice challenged with thiourea (TU, 5 mg/kg i.p., PBS/TU) and treated or not (PBS) with the KvLQT1 activator R-L3 (R-L3/TU) stained with the ATI markers podoplanin (A) , n = 50 images, <t>AQP5</t> (B) , n = 59–60 or ATII marker pro-SPC (C) , n = 48–51). Nuclei were stained by DAPI. Quantification (right panels) of podoplanin, AQP5, and pro-SPC marker intensities was made with a protocol exploited by ICY software. Values are presented as means ± SEM. Non-parametric Mann-Whitney t-test (Agostino/Pearson normality test: negative) for panel (A) . One-way ANOVA non-parametric comparison test (normality Agostino/Pearson test: negative) for panel (B , C) . * p < .05, ** p < .01 or **** p < .0001 vs . PBS/PBS, ## p < .01 vs . PBS/TU.
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    Impact of KvLQT1 activation on alveolar type I (AT1) and II (ATII) epithelial cell markers in thiourea-challenged WT mice. Representative immunofluorescence images (left panels) of lung sections (5 μm, Scale: 100 μm) from WT control mice (PBS/PBS), and WT mice challenged with thiourea (TU, 5 mg/kg i.p., PBS/TU) and treated or not (PBS) with the KvLQT1 activator R-L3 (R-L3/TU) stained with the ATI markers podoplanin (A) , n = 50 images, <t>AQP5</t> (B) , n = 59–60 or ATII marker pro-SPC (C) , n = 48–51). Nuclei were stained by DAPI. Quantification (right panels) of podoplanin, AQP5, and pro-SPC marker intensities was made with a protocol exploited by ICY software. Values are presented as means ± SEM. Non-parametric Mann-Whitney t-test (Agostino/Pearson normality test: negative) for panel (A) . One-way ANOVA non-parametric comparison test (normality Agostino/Pearson test: negative) for panel (B , C) . * p < .05, ** p < .01 or **** p < .0001 vs . PBS/PBS, ## p < .01 vs . PBS/TU.
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    Impact of KvLQT1 activation on alveolar type I (AT1) and II (ATII) epithelial cell markers in thiourea-challenged WT mice. Representative immunofluorescence images (left panels) of lung sections (5 μm, Scale: 100 μm) from WT control mice (PBS/PBS), and WT mice challenged with thiourea (TU, 5 mg/kg i.p., PBS/TU) and treated or not (PBS) with the KvLQT1 activator R-L3 (R-L3/TU) stained with the ATI markers podoplanin (A) , n = 50 images, <t>AQP5</t> (B) , n = 59–60 or ATII marker pro-SPC (C) , n = 48–51). Nuclei were stained by DAPI. Quantification (right panels) of podoplanin, AQP5, and pro-SPC marker intensities was made with a protocol exploited by ICY software. Values are presented as means ± SEM. Non-parametric Mann-Whitney t-test (Agostino/Pearson normality test: negative) for panel (A) . One-way ANOVA non-parametric comparison test (normality Agostino/Pearson test: negative) for panel (B , C) . * p < .05, ** p < .01 or **** p < .0001 vs . PBS/PBS, ## p < .01 vs . PBS/TU.
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    Spatiotemporal expression of CXC-chemokine receptor 4 (CXCR4) and its ligand, CXCL12, and developmental genes in embryonic organs. ( A ) Schematic diagram of embryonic submandibular gland (eSMG) isolation and ex vivo culture. ( B ) Ex vivo branching morphogenesis of eSMGs from embryonic day (E) 13 to 17, showing epithelial growth and retraction of mesenchyme. Scale bars: 500 µm. ( C ) Temporal mRNA expression patterns of keratin 7 ( Krt7 ), <t>aquaporin</t> <t>5</t> ( <t>Aqp5</t> ), e-cadherin ( Cdh1 ), Krt15 , Cxcr4 , and Cxcl12 were measured from E13 to E17 by qPCR ( n = 3). ( D ) Epithelial (Epi) and mesenchymal (Mes) expression of Cxcr4 , Cxcl12 , odd-skipped related transcription factor 1 ( Osr1 ), and Cdh1 were quantified by qPCR at E13. The comparative C t values are expressed as fold increase relative to the epithelium ( n = 3). ( E ) Representative images showing expression of CXCR4 and CXCL12 in eSMG (upper) and their colocalization (lower) ( n = 3, scale bar: 500 µm). ( F ) Representative immunofluorescence images of CXCR4 and CXCL12 expression in E12 embryonic lung and pancreas ( n = 4); whole view (left two panels; scale bar: 500 µm) and magnified lumen structures (right two panels; scale bar: 50 µm). Data are presented as the mean ± SEM; * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, NS: not significant; two-way ANOVA ( C ); t -test ( D ).
    Rabbit Polyclonal Anti Aqp5 Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Spatiotemporal expression of CXC-chemokine receptor 4 (CXCR4) and its ligand, CXCL12, and developmental genes in embryonic organs. ( A ) Schematic diagram of embryonic submandibular gland (eSMG) isolation and ex vivo culture. ( B ) Ex vivo branching morphogenesis of eSMGs from embryonic day (E) 13 to 17, showing epithelial growth and retraction of mesenchyme. Scale bars: 500 µm. ( C ) Temporal mRNA expression patterns of keratin 7 ( Krt7 ), <t>aquaporin</t> <t>5</t> ( <t>Aqp5</t> ), e-cadherin ( Cdh1 ), Krt15 , Cxcr4 , and Cxcl12 were measured from E13 to E17 by qPCR ( n = 3). ( D ) Epithelial (Epi) and mesenchymal (Mes) expression of Cxcr4 , Cxcl12 , odd-skipped related transcription factor 1 ( Osr1 ), and Cdh1 were quantified by qPCR at E13. The comparative C t values are expressed as fold increase relative to the epithelium ( n = 3). ( E ) Representative images showing expression of CXCR4 and CXCL12 in eSMG (upper) and their colocalization (lower) ( n = 3, scale bar: 500 µm). ( F ) Representative immunofluorescence images of CXCR4 and CXCL12 expression in E12 embryonic lung and pancreas ( n = 4); whole view (left two panels; scale bar: 500 µm) and magnified lumen structures (right two panels; scale bar: 50 µm). Data are presented as the mean ± SEM; * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, NS: not significant; two-way ANOVA ( C ); t -test ( D ).
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    Histological analysis of salivary glands. ( A ) Representative histological images of PAS and MT staining. More mucin-containing acini and less periductal fibrosis were observed in the RI+KGF-1 group than in the RI group. ( B ) Representative immunohistochemical images showing HIF1a, salivary epithelial cells <t>(AQP5</t> stained) and endothelial cells (CD31 stained). The expression of HIF1a was higher and the expressions of AQP5 and CD31 were lower in the RI group than in treatment naïve controls. HIF1a staining intensities was lesser, and AQP5 and CD31 staining intensities were greater in the RI+KGF-1 group than in the RI group. (N; treatment naïve control group, RI; RI group, KGF; RI+KGF-1 group). Results are presented as means ± SDs; *, vs. treatment naïve controls; # , vs. the RI group. * p < 0.05, # p < 0.05 ( n = 6 mice per group) (N; treatment naïve controls, RI; RI group, KGF; RI+KGF-1 group), Scale bar: 50 μm. ( C ) More mucin-containing acini and less periductal fibrosis were observed in the RI+KGF-1 group than in the RI group. The expression of HIF1a was higher and the expressions of AQP5 and CD31 were lower in the RI group than in treatment naïve controls. HIF1a staining intensities was lesser, and AQP5 and CD31 staining intensities were greater in the RI+KGF-1 group than in the RI group. (N; treatment naïve control group, RI; RI group, KGF; RI+KGF-1 group). Results are presented as means ± SDs; *, vs. treatment naïve controls; # , vs. the RI group. * p < 0.05, # p < 0.05 ( n = 6 mice per group) (N; treatment naïve controls, RI; RI group, KGF; RI+KGF-1 group), Scale bar: 50 μm.
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    Image Search Results


    Impact of KvLQT1 activation on alveolar type I (AT1) and II (ATII) epithelial cell markers in thiourea-challenged WT mice. Representative immunofluorescence images (left panels) of lung sections (5 μm, Scale: 100 μm) from WT control mice (PBS/PBS), and WT mice challenged with thiourea (TU, 5 mg/kg i.p., PBS/TU) and treated or not (PBS) with the KvLQT1 activator R-L3 (R-L3/TU) stained with the ATI markers podoplanin (A) , n = 50 images, AQP5 (B) , n = 59–60 or ATII marker pro-SPC (C) , n = 48–51). Nuclei were stained by DAPI. Quantification (right panels) of podoplanin, AQP5, and pro-SPC marker intensities was made with a protocol exploited by ICY software. Values are presented as means ± SEM. Non-parametric Mann-Whitney t-test (Agostino/Pearson normality test: negative) for panel (A) . One-way ANOVA non-parametric comparison test (normality Agostino/Pearson test: negative) for panel (B , C) . * p < .05, ** p < .01 or **** p < .0001 vs . PBS/PBS, ## p < .01 vs . PBS/TU.

    Journal: Frontiers in Physiology

    Article Title: Impact of KvLQT1 potassium channel modulation on alveolar fluid homeostasis in an animal model of thiourea-induced lung edema

    doi: 10.3389/fphys.2022.1069466

    Figure Lengend Snippet: Impact of KvLQT1 activation on alveolar type I (AT1) and II (ATII) epithelial cell markers in thiourea-challenged WT mice. Representative immunofluorescence images (left panels) of lung sections (5 μm, Scale: 100 μm) from WT control mice (PBS/PBS), and WT mice challenged with thiourea (TU, 5 mg/kg i.p., PBS/TU) and treated or not (PBS) with the KvLQT1 activator R-L3 (R-L3/TU) stained with the ATI markers podoplanin (A) , n = 50 images, AQP5 (B) , n = 59–60 or ATII marker pro-SPC (C) , n = 48–51). Nuclei were stained by DAPI. Quantification (right panels) of podoplanin, AQP5, and pro-SPC marker intensities was made with a protocol exploited by ICY software. Values are presented as means ± SEM. Non-parametric Mann-Whitney t-test (Agostino/Pearson normality test: negative) for panel (A) . One-way ANOVA non-parametric comparison test (normality Agostino/Pearson test: negative) for panel (B , C) . * p < .05, ** p < .01 or **** p < .0001 vs . PBS/PBS, ## p < .01 vs . PBS/TU.

    Article Snippet: For AQP5 detection by immunostaining, tissue sections were processed for heat-induced antigenic retrieval (with citrate buffer, pH 3.45) and then blocked with a solution of PBS +10% FBS (Saradigm, United States) + 10% BSA (Sigma-Aldrich) + .01% Triton X-100 (Amersham Biosciences, Sweden) for 1 h. Slides were then incubated overnight at 4°C with an anti-AQP5 rabbit polyclonal antibody (1:100, #AQP-005, Alomone Labs, Israël).

    Techniques: Activation Assay, Immunofluorescence, Staining, Marker, Software, MANN-WHITNEY

    Spatiotemporal expression of CXC-chemokine receptor 4 (CXCR4) and its ligand, CXCL12, and developmental genes in embryonic organs. ( A ) Schematic diagram of embryonic submandibular gland (eSMG) isolation and ex vivo culture. ( B ) Ex vivo branching morphogenesis of eSMGs from embryonic day (E) 13 to 17, showing epithelial growth and retraction of mesenchyme. Scale bars: 500 µm. ( C ) Temporal mRNA expression patterns of keratin 7 ( Krt7 ), aquaporin 5 ( Aqp5 ), e-cadherin ( Cdh1 ), Krt15 , Cxcr4 , and Cxcl12 were measured from E13 to E17 by qPCR ( n = 3). ( D ) Epithelial (Epi) and mesenchymal (Mes) expression of Cxcr4 , Cxcl12 , odd-skipped related transcription factor 1 ( Osr1 ), and Cdh1 were quantified by qPCR at E13. The comparative C t values are expressed as fold increase relative to the epithelium ( n = 3). ( E ) Representative images showing expression of CXCR4 and CXCL12 in eSMG (upper) and their colocalization (lower) ( n = 3, scale bar: 500 µm). ( F ) Representative immunofluorescence images of CXCR4 and CXCL12 expression in E12 embryonic lung and pancreas ( n = 4); whole view (left two panels; scale bar: 500 µm) and magnified lumen structures (right two panels; scale bar: 50 µm). Data are presented as the mean ± SEM; * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, NS: not significant; two-way ANOVA ( C ); t -test ( D ).

    Journal: International Journal of Molecular Sciences

    Article Title: CXCR4 Regulates Temporal Differentiation via PRC1 Complex in Organogenesis of Epithelial Glands

    doi: 10.3390/ijms22020619

    Figure Lengend Snippet: Spatiotemporal expression of CXC-chemokine receptor 4 (CXCR4) and its ligand, CXCL12, and developmental genes in embryonic organs. ( A ) Schematic diagram of embryonic submandibular gland (eSMG) isolation and ex vivo culture. ( B ) Ex vivo branching morphogenesis of eSMGs from embryonic day (E) 13 to 17, showing epithelial growth and retraction of mesenchyme. Scale bars: 500 µm. ( C ) Temporal mRNA expression patterns of keratin 7 ( Krt7 ), aquaporin 5 ( Aqp5 ), e-cadherin ( Cdh1 ), Krt15 , Cxcr4 , and Cxcl12 were measured from E13 to E17 by qPCR ( n = 3). ( D ) Epithelial (Epi) and mesenchymal (Mes) expression of Cxcr4 , Cxcl12 , odd-skipped related transcription factor 1 ( Osr1 ), and Cdh1 were quantified by qPCR at E13. The comparative C t values are expressed as fold increase relative to the epithelium ( n = 3). ( E ) Representative images showing expression of CXCR4 and CXCL12 in eSMG (upper) and their colocalization (lower) ( n = 3, scale bar: 500 µm). ( F ) Representative immunofluorescence images of CXCR4 and CXCL12 expression in E12 embryonic lung and pancreas ( n = 4); whole view (left two panels; scale bar: 500 µm) and magnified lumen structures (right two panels; scale bar: 50 µm). Data are presented as the mean ± SEM; * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, NS: not significant; two-way ANOVA ( C ); t -test ( D ).

    Article Snippet: The following primary antibodies were used in the procedures: rat monoclonal anti-CXCR4 antibody (R&D Systems, MAB21651), rabbit monoclonal anti-KRT7 antibody (Abcam, ab181598; Cambridge, UK); rabbit monoclonal anti-CDH1 antibody (CST, 3195; Beverly, MA, USA), rabbit monoclonal anti-H2AK119ub antibody (CST, 8240); mouse monoclonal anti-CXCL12 antibody (Novus Biologicals, MAB350; Littleton, CO, USA), rabbit polyclonal anti-AQP5 antibody (Alomone Labs, AQP-005; Jerusalem, Israel), rabbit monoclonal anti-CASP3 antibody (CST, 9664), and rat monoclonal anti-KI67 antibody (ThermoFisher, 14-5698-82).

    Techniques: Expressing, Isolation, Ex Vivo, Immunofluorescence

    AMD3100-induced precocious differentiation of epithelial cells. ( A , B ) Representative contour tracing ( A ) and bud number changes ( B ) of control and AMD3100-treated eSMGs during 48 h at 6-h intervals ( n = 3). ( C ) EdU staining results at 6 and 24 h after AMD3100 treatment. EdU in green and PNA in gray ( n = 4, scale bar: 500 µm). ( D ) Immunostaining results of Ki67 (red) and F-actin (green) in acini and duct of eSMGs 24 h after AMD3100 treatment. Morphologies of acinar buds and duct cells are outlined with white dotted lines. Scale bar: 50 µm. ( E ) Duct widths of control and AMD3100-treated eSMGs were visualized via F-actin-based intensity profiles of horizontal sectioning of ducts 24 h after the treatment. ( F ) Duct widths of control and AMD3100-treated eSMGs were quantified 24 h after the treatment ( n = 9). ( G ) Immunostaining results of AQP5 (green) and KRT7 (red). Magnified regions of acinar buds are marked with white dotted squares. The white arrows (middle panels) indicate areas with the highest AQP5 expression ( n = 4, scale bar: left, 100 µm; middle and right, 50 µm). Data are presented as the mean ± SEM; * p < 0.05, **** p < 0.0001; t -test.

    Journal: International Journal of Molecular Sciences

    Article Title: CXCR4 Regulates Temporal Differentiation via PRC1 Complex in Organogenesis of Epithelial Glands

    doi: 10.3390/ijms22020619

    Figure Lengend Snippet: AMD3100-induced precocious differentiation of epithelial cells. ( A , B ) Representative contour tracing ( A ) and bud number changes ( B ) of control and AMD3100-treated eSMGs during 48 h at 6-h intervals ( n = 3). ( C ) EdU staining results at 6 and 24 h after AMD3100 treatment. EdU in green and PNA in gray ( n = 4, scale bar: 500 µm). ( D ) Immunostaining results of Ki67 (red) and F-actin (green) in acini and duct of eSMGs 24 h after AMD3100 treatment. Morphologies of acinar buds and duct cells are outlined with white dotted lines. Scale bar: 50 µm. ( E ) Duct widths of control and AMD3100-treated eSMGs were visualized via F-actin-based intensity profiles of horizontal sectioning of ducts 24 h after the treatment. ( F ) Duct widths of control and AMD3100-treated eSMGs were quantified 24 h after the treatment ( n = 9). ( G ) Immunostaining results of AQP5 (green) and KRT7 (red). Magnified regions of acinar buds are marked with white dotted squares. The white arrows (middle panels) indicate areas with the highest AQP5 expression ( n = 4, scale bar: left, 100 µm; middle and right, 50 µm). Data are presented as the mean ± SEM; * p < 0.05, **** p < 0.0001; t -test.

    Article Snippet: The following primary antibodies were used in the procedures: rat monoclonal anti-CXCR4 antibody (R&D Systems, MAB21651), rabbit monoclonal anti-KRT7 antibody (Abcam, ab181598; Cambridge, UK); rabbit monoclonal anti-CDH1 antibody (CST, 3195; Beverly, MA, USA), rabbit monoclonal anti-H2AK119ub antibody (CST, 8240); mouse monoclonal anti-CXCL12 antibody (Novus Biologicals, MAB350; Littleton, CO, USA), rabbit polyclonal anti-AQP5 antibody (Alomone Labs, AQP-005; Jerusalem, Israel), rabbit monoclonal anti-CASP3 antibody (CST, 9664), and rat monoclonal anti-KI67 antibody (ThermoFisher, 14-5698-82).

    Techniques: Staining, Immunostaining, Expressing

    eSMGs lacking PRC1 develop precocious differentiation in acini and ducts. ( A ) Schematic diagram of AMD3100-induced effect on organogenesis. Down-regulation of PRC1 genes leads to the inability of methylation site recognition by chromobox 8 (CBX8), a polycomb group protein ( 1 ), resulting in failure of the ubiquitin-ligating activity of ring finger protein 1 (RING1) ( 2 ) and thus, disabled DNA condensation ( 3 ). Hence, the developmental genes are readily transcribed by RNA polymerase II. ( B ) Representative images showing ubiquitin expression at Lys119 of histone 2A (H2AK119ub) in control and AMD3100-treated eSMGs 24 h after the treatment ( n = 4, scale bar: 50 µm). ( C ) Mean fluorescence intensities of H2AK119ub in epithelial buds per z -stack of 0.91 µm (25 stacks per end bud). False discovery rate (FDR) q -values of the differences in H2AK119ub expression at each of the 25 stacks are shown in the heat map, in which 23 out of 25 stacks marked a significant difference between control and AMD3100-treated eSMGs. -log( q -value) > 1.3 is considered significant ( q < 0.05; t -test with 5% FDR correction). The heat map (right) shows the color profile ( n = 4). ( D ) Methyl-seq results from control and AMD3100-treated eSMGs. Ten eSMGs per group were homogenized and analyzed. ( E ) Relative DAPI intensities of end buds in control, AMD3100-, and UNC3866-treated groups are expressed as a colored heat map (0–65,535 gray-value). Magnified regions are marked with white dotted squares ( n = 4, scale bar: 20 µm). ( F ) Quantification of DAPI intensities in nuclei of control (CON), AMD3100 (AMD)-, and UNC3866 (UNC)-treated eSMGs ( n = 18). ( G ) Immunostaining results of AQP5 (yellow) and KRT7 (gray) in eSMGs treated with AMD3100 or UNC3866. eSMGs were fixed and immunostained 24 h after the treatment ( n = 4, scale bar: upper panel, 50 µm; lower panel, 500 µm). ( H ) Quantification of AQP5 intensities in acinar buds of eSMGs treated with AMD3100 (AMD) or UNC3866 (UNC) ( n = 30). ( I ) Quantification of KRT7 intensities in ducts of eSMGs treated with AMD3100 (AMD) or UNC3866 (UNC) ( n = 4). Data are presented as the mean ± SEM; ** p < 0.01, *** p < 0.001, **** p < 0.0001; one-way ANOVA.

    Journal: International Journal of Molecular Sciences

    Article Title: CXCR4 Regulates Temporal Differentiation via PRC1 Complex in Organogenesis of Epithelial Glands

    doi: 10.3390/ijms22020619

    Figure Lengend Snippet: eSMGs lacking PRC1 develop precocious differentiation in acini and ducts. ( A ) Schematic diagram of AMD3100-induced effect on organogenesis. Down-regulation of PRC1 genes leads to the inability of methylation site recognition by chromobox 8 (CBX8), a polycomb group protein ( 1 ), resulting in failure of the ubiquitin-ligating activity of ring finger protein 1 (RING1) ( 2 ) and thus, disabled DNA condensation ( 3 ). Hence, the developmental genes are readily transcribed by RNA polymerase II. ( B ) Representative images showing ubiquitin expression at Lys119 of histone 2A (H2AK119ub) in control and AMD3100-treated eSMGs 24 h after the treatment ( n = 4, scale bar: 50 µm). ( C ) Mean fluorescence intensities of H2AK119ub in epithelial buds per z -stack of 0.91 µm (25 stacks per end bud). False discovery rate (FDR) q -values of the differences in H2AK119ub expression at each of the 25 stacks are shown in the heat map, in which 23 out of 25 stacks marked a significant difference between control and AMD3100-treated eSMGs. -log( q -value) > 1.3 is considered significant ( q < 0.05; t -test with 5% FDR correction). The heat map (right) shows the color profile ( n = 4). ( D ) Methyl-seq results from control and AMD3100-treated eSMGs. Ten eSMGs per group were homogenized and analyzed. ( E ) Relative DAPI intensities of end buds in control, AMD3100-, and UNC3866-treated groups are expressed as a colored heat map (0–65,535 gray-value). Magnified regions are marked with white dotted squares ( n = 4, scale bar: 20 µm). ( F ) Quantification of DAPI intensities in nuclei of control (CON), AMD3100 (AMD)-, and UNC3866 (UNC)-treated eSMGs ( n = 18). ( G ) Immunostaining results of AQP5 (yellow) and KRT7 (gray) in eSMGs treated with AMD3100 or UNC3866. eSMGs were fixed and immunostained 24 h after the treatment ( n = 4, scale bar: upper panel, 50 µm; lower panel, 500 µm). ( H ) Quantification of AQP5 intensities in acinar buds of eSMGs treated with AMD3100 (AMD) or UNC3866 (UNC) ( n = 30). ( I ) Quantification of KRT7 intensities in ducts of eSMGs treated with AMD3100 (AMD) or UNC3866 (UNC) ( n = 4). Data are presented as the mean ± SEM; ** p < 0.01, *** p < 0.001, **** p < 0.0001; one-way ANOVA.

    Article Snippet: The following primary antibodies were used in the procedures: rat monoclonal anti-CXCR4 antibody (R&D Systems, MAB21651), rabbit monoclonal anti-KRT7 antibody (Abcam, ab181598; Cambridge, UK); rabbit monoclonal anti-CDH1 antibody (CST, 3195; Beverly, MA, USA), rabbit monoclonal anti-H2AK119ub antibody (CST, 8240); mouse monoclonal anti-CXCL12 antibody (Novus Biologicals, MAB350; Littleton, CO, USA), rabbit polyclonal anti-AQP5 antibody (Alomone Labs, AQP-005; Jerusalem, Israel), rabbit monoclonal anti-CASP3 antibody (CST, 9664), and rat monoclonal anti-KI67 antibody (ThermoFisher, 14-5698-82).

    Techniques: Methylation, Activity Assay, Expressing, Fluorescence, Immunostaining

    Histological analysis of salivary glands. ( A ) Representative histological images of PAS and MT staining. More mucin-containing acini and less periductal fibrosis were observed in the RI+KGF-1 group than in the RI group. ( B ) Representative immunohistochemical images showing HIF1a, salivary epithelial cells (AQP5 stained) and endothelial cells (CD31 stained). The expression of HIF1a was higher and the expressions of AQP5 and CD31 were lower in the RI group than in treatment naïve controls. HIF1a staining intensities was lesser, and AQP5 and CD31 staining intensities were greater in the RI+KGF-1 group than in the RI group. (N; treatment naïve control group, RI; RI group, KGF; RI+KGF-1 group). Results are presented as means ± SDs; *, vs. treatment naïve controls; # , vs. the RI group. * p < 0.05, # p < 0.05 ( n = 6 mice per group) (N; treatment naïve controls, RI; RI group, KGF; RI+KGF-1 group), Scale bar: 50 μm. ( C ) More mucin-containing acini and less periductal fibrosis were observed in the RI+KGF-1 group than in the RI group. The expression of HIF1a was higher and the expressions of AQP5 and CD31 were lower in the RI group than in treatment naïve controls. HIF1a staining intensities was lesser, and AQP5 and CD31 staining intensities were greater in the RI+KGF-1 group than in the RI group. (N; treatment naïve control group, RI; RI group, KGF; RI+KGF-1 group). Results are presented as means ± SDs; *, vs. treatment naïve controls; # , vs. the RI group. * p < 0.05, # p < 0.05 ( n = 6 mice per group) (N; treatment naïve controls, RI; RI group, KGF; RI+KGF-1 group), Scale bar: 50 μm.

    Journal: International Journal of Environmental Research and Public Health

    Article Title: Keratinocyte Growth Factor-1 Protects Radioiodine-Induced Salivary Gland Dysfunction in Mice

    doi: 10.3390/ijerph17176322

    Figure Lengend Snippet: Histological analysis of salivary glands. ( A ) Representative histological images of PAS and MT staining. More mucin-containing acini and less periductal fibrosis were observed in the RI+KGF-1 group than in the RI group. ( B ) Representative immunohistochemical images showing HIF1a, salivary epithelial cells (AQP5 stained) and endothelial cells (CD31 stained). The expression of HIF1a was higher and the expressions of AQP5 and CD31 were lower in the RI group than in treatment naïve controls. HIF1a staining intensities was lesser, and AQP5 and CD31 staining intensities were greater in the RI+KGF-1 group than in the RI group. (N; treatment naïve control group, RI; RI group, KGF; RI+KGF-1 group). Results are presented as means ± SDs; *, vs. treatment naïve controls; # , vs. the RI group. * p < 0.05, # p < 0.05 ( n = 6 mice per group) (N; treatment naïve controls, RI; RI group, KGF; RI+KGF-1 group), Scale bar: 50 μm. ( C ) More mucin-containing acini and less periductal fibrosis were observed in the RI+KGF-1 group than in the RI group. The expression of HIF1a was higher and the expressions of AQP5 and CD31 were lower in the RI group than in treatment naïve controls. HIF1a staining intensities was lesser, and AQP5 and CD31 staining intensities were greater in the RI+KGF-1 group than in the RI group. (N; treatment naïve control group, RI; RI group, KGF; RI+KGF-1 group). Results are presented as means ± SDs; *, vs. treatment naïve controls; # , vs. the RI group. * p < 0.05, # p < 0.05 ( n = 6 mice per group) (N; treatment naïve controls, RI; RI group, KGF; RI+KGF-1 group), Scale bar: 50 μm.

    Article Snippet: They were incubated overnight at 4 °C with rabbit polyclonal antibodies against aquaporin 5 (AQP5) (diluted 1:200; Alomone Labs, Jerusalem, Israel), cluster of differentiation 31 (CD31), and hypoxia-inducible factor 1-alpha (HIF1a) (diluted 1:200; Santa Cruz, CA, USA) using an LSAB kit (Dako, Carpinteria, CA, USA) counterstained with Haemotoxylin and Eosin (H&E), dehydrated, and mounted.

    Techniques: Staining, Immunohistochemical staining, Expressing

    Pig mandibular gland. AQP5 binding sites at duct (*) and demilune (↑) level in CM, FP and CP groups.

    Journal: Animals : an Open Access Journal from MDPI

    Article Title: Influence of Different Feed Physical Forms on Mandibular Gland in Growing Pigs

    doi: 10.3390/ani10050910

    Figure Lengend Snippet: Pig mandibular gland. AQP5 binding sites at duct (*) and demilune (↑) level in CM, FP and CP groups.

    Article Snippet: Subsequently, once the excess of reagent has been removed from the sections, they were incubated, overnight at RT, each with one of the primary antibodies: rabbit polyclonal antibody anti-APLN (1:100, NBP2-31176, Novus Biologicals, Littleton, CO, USA), mouse monoclonal antibody anti-APLNR (1:100, sc-517300, Santa Cruz Biotechnology, Santa Cruz, CA, USA), and rabbit polyclonal antibody anti-AQP5 (1:100, AQP-005, Alomone Labs, Jerusalem 9104201, Israel).

    Techniques: Binding Assay