6986s  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc 6986s
    6986s, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    β site app cleavage enzyme  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc β site app cleavage enzyme
    β Site App Cleavage Enzyme, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    app e4h1u rabbit mab against total app protein  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc app e4h1u rabbit mab against total app protein
    App E4h1u Rabbit Mab Against Total App Protein, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    6986s  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc 6986s
    6986s, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    p app thr688  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc p app thr688
    P App Thr688, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    rabbit anti human carboxy terminus of app  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc rabbit anti human carboxy terminus of app
    Primary and secondary antibodies used for Immunoblotting
    Rabbit Anti Human Carboxy Terminus Of App, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Anti-malaria drug artesunate prevents development of amyloid-β pathology in mice by upregulating PICALM at the blood-brain barrier"

    Article Title: Anti-malaria drug artesunate prevents development of amyloid-β pathology in mice by upregulating PICALM at the blood-brain barrier

    Journal: Molecular Neurodegeneration

    doi: 10.1186/s13024-023-00597-5

    Primary and secondary antibodies used for Immunoblotting
    Figure Legend Snippet: Primary and secondary antibodies used for Immunoblotting

    Techniques Used: Sequencing

    app  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc app
    Primary and secondary antibodies used for Immunoblotting
    App, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Anti-malaria drug artesunate prevents development of amyloid-β pathology in mice by upregulating PICALM at the blood-brain barrier"

    Article Title: Anti-malaria drug artesunate prevents development of amyloid-β pathology in mice by upregulating PICALM at the blood-brain barrier

    Journal: Molecular Neurodegeneration

    doi: 10.1186/s13024-023-00597-5

    Primary and secondary antibodies used for Immunoblotting
    Figure Legend Snippet: Primary and secondary antibodies used for Immunoblotting

    Techniques Used: Sequencing

    mouse app  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc mouse app
    Primary and secondary antibodies used for Immunoblotting
    Mouse App, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Anti-malaria drug artesunate prevents development of amyloid-β pathology in mice by upregulating PICALM at the blood-brain barrier"

    Article Title: Anti-malaria drug artesunate prevents development of amyloid-β pathology in mice by upregulating PICALM at the blood-brain barrier

    Journal: Molecular Neurodegeneration

    doi: 10.1186/s13024-023-00597-5

    Primary and secondary antibodies used for Immunoblotting
    Figure Legend Snippet: Primary and secondary antibodies used for Immunoblotting

    Techniques Used: Sequencing

    app  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc app
    Primers used for qRT-PCR
    App, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Studies on the mechanism of Toxoplasma gondii Chinese 1 genotype Wh6 strain causing mice abnormal cognitive behavior"

    Article Title: Studies on the mechanism of Toxoplasma gondii Chinese 1 genotype Wh6 strain causing mice abnormal cognitive behavior

    Journal: Parasites & Vectors

    doi: 10.1186/s13071-022-05618-8

    Primers used for qRT-PCR
    Figure Legend Snippet: Primers used for qRT-PCR

    Techniques Used:

    TgCtwh6 infection increases APP, BACE1 and Aβ production in mouse hippocampus tissue. First, the hippocampus tissue section was analyzed by immunhistochemistry method. The stained yellow positive areas were observed under a light microscope at a magnification of 20 × 100 and 40 × 100 (scale bars, 50 μm and 20 μm, respectively) ( A ). The Aβ protein expression in the mouse hippocampal zone was evaluated with semiquantitative method ( B ). Then, the hippocampus tissue section was stained with thioflavin S solution followed by counterstaining nuclei with DAPI. The stained bright green plaques were observed under a fluorescence microscope at a magnification of 20 × 100 and 40 × 100 (scale bars, 50 μm and 20 μm, respectively) ( C ). The Aβ protein expression in the mouse hippocampal zone was estimated using semiquantitative method ( D ). Third, the mouse right brain hippocampus tissues were lysed and proteins were extracted; APP and BACE1 protein were detected by western blotting and then analyzed semiquantitatively ( E – G ). Finally, the RNA of the mouse right brain hippocampus tissue was extracted and then reversely transcribed to cDNA. APP and BACE1 gene expressions were assessed by qRT-PCR and then analyzed semiquantitatively (H, I). Data were represented as mean ± SEM and were analyzed by two-tailed Student’s t -test for comparing the difference between the two groups ( n = 3 each group). * P < 0. 05, ** P < 0.01, *** P < 0.001
    Figure Legend Snippet: TgCtwh6 infection increases APP, BACE1 and Aβ production in mouse hippocampus tissue. First, the hippocampus tissue section was analyzed by immunhistochemistry method. The stained yellow positive areas were observed under a light microscope at a magnification of 20 × 100 and 40 × 100 (scale bars, 50 μm and 20 μm, respectively) ( A ). The Aβ protein expression in the mouse hippocampal zone was evaluated with semiquantitative method ( B ). Then, the hippocampus tissue section was stained with thioflavin S solution followed by counterstaining nuclei with DAPI. The stained bright green plaques were observed under a fluorescence microscope at a magnification of 20 × 100 and 40 × 100 (scale bars, 50 μm and 20 μm, respectively) ( C ). The Aβ protein expression in the mouse hippocampal zone was estimated using semiquantitative method ( D ). Third, the mouse right brain hippocampus tissues were lysed and proteins were extracted; APP and BACE1 protein were detected by western blotting and then analyzed semiquantitatively ( E – G ). Finally, the RNA of the mouse right brain hippocampus tissue was extracted and then reversely transcribed to cDNA. APP and BACE1 gene expressions were assessed by qRT-PCR and then analyzed semiquantitatively (H, I). Data were represented as mean ± SEM and were analyzed by two-tailed Student’s t -test for comparing the difference between the two groups ( n = 3 each group). * P < 0. 05, ** P < 0.01, *** P < 0.001

    Techniques Used: Infection, Staining, Light Microscopy, Expressing, Fluorescence, Microscopy, Western Blot, Quantitative RT-PCR, Two Tailed Test

    TgCtwh6 tachyzoites upregulate APP, BACE1 and Aβ expression in HT22. After TgCtwh6 tachyzoites had been infected HT22 for 24 h, the HT22 were lysed and proteins were extracted. The expression of BACE1 and APP proteins was measured by western blotting and then analyzed with semiquantitative method ( A – C ). Moreover, RNA in the infected HT22 was extracted and reversely transcribed to cDNA. The expressions of BACE1 and APP genes was assessed by qRT-PCR and then semiquantitatively evaluated ( D , E ). Furthermore, after the HT22 cells had been fixed and the cytomembranes penetrated by Triton, the BACE1 and Aβ proteins were visualized in situ by immunofluorescence staining and observed under a fluorescence microscope at a magnification of 40 × 100 (scale bars, 50 μm). The percentage of positively stained cells was calculated using morphometric software ( F – G ). Data were represented as mean ± SEM and were analyzed by two-tailed Student’s t -test for comparing the difference between the two groups ( n = 3 each group). * P < 0. 05, ** P < 0.01, *** P < 0.001
    Figure Legend Snippet: TgCtwh6 tachyzoites upregulate APP, BACE1 and Aβ expression in HT22. After TgCtwh6 tachyzoites had been infected HT22 for 24 h, the HT22 were lysed and proteins were extracted. The expression of BACE1 and APP proteins was measured by western blotting and then analyzed with semiquantitative method ( A – C ). Moreover, RNA in the infected HT22 was extracted and reversely transcribed to cDNA. The expressions of BACE1 and APP genes was assessed by qRT-PCR and then semiquantitatively evaluated ( D , E ). Furthermore, after the HT22 cells had been fixed and the cytomembranes penetrated by Triton, the BACE1 and Aβ proteins were visualized in situ by immunofluorescence staining and observed under a fluorescence microscope at a magnification of 40 × 100 (scale bars, 50 μm). The percentage of positively stained cells was calculated using morphometric software ( F – G ). Data were represented as mean ± SEM and were analyzed by two-tailed Student’s t -test for comparing the difference between the two groups ( n = 3 each group). * P < 0. 05, ** P < 0.01, *** P < 0.001

    Techniques Used: Expressing, Infection, Western Blot, Quantitative RT-PCR, In Situ, Immunofluorescence, Staining, Fluorescence, Microscopy, Software, Two Tailed Test

    TgCtwh6 tachyzoites induce Aβ production by activating NF-κB signaling pathway in HT22. HT22 cells pre-treated with or without PDTC for 12 h were challenged with or without TgCtwh6 tachyzoites for 24 h. Then, after the HT22 cells had been collected and lysed, the proteins in HT22 were extracted. The expressions of NF-κBp65, p -NF-κBp65, APP and BACE1 were detected by western blotting and then semiauantitative analyzed ( A – E ). Additionally, after the HT22 cells had been fixed and the cytomembranes penetrated by Triton, the NF-κBp65, p -NF-κBp65, BACE1 and Aβ proteins were visualized in situ by immunofluorescence staining and were observed under a fluorescence microscope at a magnification of 40 × 100. The percentage of positively stained cells was calculated using morphometric software ( F – M ). Data were represented as mean ± SEM and were analyzed by one-way ANOVA followed by Bonferroni’s post hoc test for multiple comparisons ( n = 3 each group). * P < 0. 05, ** P < 0.01, *** P < 0.001
    Figure Legend Snippet: TgCtwh6 tachyzoites induce Aβ production by activating NF-κB signaling pathway in HT22. HT22 cells pre-treated with or without PDTC for 12 h were challenged with or without TgCtwh6 tachyzoites for 24 h. Then, after the HT22 cells had been collected and lysed, the proteins in HT22 were extracted. The expressions of NF-κBp65, p -NF-κBp65, APP and BACE1 were detected by western blotting and then semiauantitative analyzed ( A – E ). Additionally, after the HT22 cells had been fixed and the cytomembranes penetrated by Triton, the NF-κBp65, p -NF-κBp65, BACE1 and Aβ proteins were visualized in situ by immunofluorescence staining and were observed under a fluorescence microscope at a magnification of 40 × 100. The percentage of positively stained cells was calculated using morphometric software ( F – M ). Data were represented as mean ± SEM and were analyzed by one-way ANOVA followed by Bonferroni’s post hoc test for multiple comparisons ( n = 3 each group). * P < 0. 05, ** P < 0.01, *** P < 0.001

    Techniques Used: Western Blot, In Situ, Immunofluorescence, Staining, Fluorescence, Microscopy, Software

    Mechanism with which TgCtwh6 infection induces mouse cognitive behavior disorder at the cellular and molecular level in our experiments. First, in vivo, each C57BL/6 mouse was infected orally with TgCtwh6 cysts. On day 90 post-infection, the infected mice manifested cognitive behavioral abnormalities. The infected mouse hippocampal tissue assay showed that neurons were disorganized in the arrangement, decreased in the number and abnormally stained in nuclei. Moreover, apoptotic cells and Aβ protein increased in the infected mouse hippocampus ( A ). TgCtwh6 tachyzoites led to HT22 apoptosis following HT22 infection for 24 h; meanwhile, TgCtwh6 tachyzoites promoted BACE1, APP and Aβ production by activating NF-κB signaling pathway in HT22. Additionally, after BV2 cells had been infected by TgCtwh6 tachyzoites for 24 h, they could drive polarization to M1 through Notch signaling pathway with production of pro-inflammatory factors, which provoked co-cultured HT22 apoptosis and APP expression ( B )
    Figure Legend Snippet: Mechanism with which TgCtwh6 infection induces mouse cognitive behavior disorder at the cellular and molecular level in our experiments. First, in vivo, each C57BL/6 mouse was infected orally with TgCtwh6 cysts. On day 90 post-infection, the infected mice manifested cognitive behavioral abnormalities. The infected mouse hippocampal tissue assay showed that neurons were disorganized in the arrangement, decreased in the number and abnormally stained in nuclei. Moreover, apoptotic cells and Aβ protein increased in the infected mouse hippocampus ( A ). TgCtwh6 tachyzoites led to HT22 apoptosis following HT22 infection for 24 h; meanwhile, TgCtwh6 tachyzoites promoted BACE1, APP and Aβ production by activating NF-κB signaling pathway in HT22. Additionally, after BV2 cells had been infected by TgCtwh6 tachyzoites for 24 h, they could drive polarization to M1 through Notch signaling pathway with production of pro-inflammatory factors, which provoked co-cultured HT22 apoptosis and APP expression ( B )

    Techniques Used: Infection, In Vivo, Staining, Cell Culture, Expressing

    anti phospho app thr668 d90b8 rabbit mab  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc anti phospho app thr668 d90b8 rabbit mab
    Anti Phospho App Thr668 D90b8 Rabbit Mab, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    rabbit anti app polyclonal antibody  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc rabbit anti app polyclonal antibody
    Rabbit Anti App Polyclonal Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    rabbit anti human carboxy terminus of app  (Cell Signaling Technology Inc)
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    Primary and secondary antibodies used for Immunoblotting

    Journal: Molecular Neurodegeneration

    Article Title: Anti-malaria drug artesunate prevents development of amyloid-β pathology in mice by upregulating PICALM at the blood-brain barrier

    doi: 10.1186/s13024-023-00597-5

    Figure Lengend Snippet: Primary and secondary antibodies used for Immunoblotting

    Article Snippet: Rabbit anti-human carboxy terminus of APP, cross-reacts with mouse APP and APP-CTFs (Cell Signaling, 76600S, 1:1000) , HRP-conjugated donkey anti-rabbit (Invitrogen, A16023,1:3000).

    Techniques: Sequencing

    Primary and secondary antibodies used for Immunoblotting

    Journal: Molecular Neurodegeneration

    Article Title: Anti-malaria drug artesunate prevents development of amyloid-β pathology in mice by upregulating PICALM at the blood-brain barrier

    doi: 10.1186/s13024-023-00597-5

    Figure Lengend Snippet: Primary and secondary antibodies used for Immunoblotting

    Article Snippet: Rabbit anti-human carboxy terminus of APP, cross-reacts with mouse APP and APP-CTFs (Cell Signaling, 76600S, 1:1000) , HRP-conjugated donkey anti-rabbit (Invitrogen, A16023,1:3000).

    Techniques: Sequencing

    Primary and secondary antibodies used for Immunoblotting

    Journal: Molecular Neurodegeneration

    Article Title: Anti-malaria drug artesunate prevents development of amyloid-β pathology in mice by upregulating PICALM at the blood-brain barrier

    doi: 10.1186/s13024-023-00597-5

    Figure Lengend Snippet: Primary and secondary antibodies used for Immunoblotting

    Article Snippet: Rabbit anti-human carboxy terminus of APP, cross-reacts with mouse APP and APP-CTFs (Cell Signaling, 76600S, 1:1000) , HRP-conjugated donkey anti-rabbit (Invitrogen, A16023,1:3000).

    Techniques: Sequencing