rabbit anti a 2b ar  (Alomone Labs)


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    Alomone Labs rabbit anti a 2b ar
    Rabbit Anti A 2b Ar, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94/100 stars

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    rabbit anti a 2b ar  (Alomone Labs)


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    Alomone Labs rabbit anti a 2b ar
    Rabbit Anti A 2b Ar, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    anti α2b adrenoreceptor  (Alomone Labs)


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    Alomone Labs anti α2b adrenoreceptor
    Anti α2b Adrenoreceptor, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    α2b  (Alomone Labs)


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    Alomone Labs α2b
    The effect of ARC 239, a specific antagonist of the <t>α2B</t> isoform, on the guanabenz-induced suppression of I CaL . ( a , b ) Representative time-courses of peak I CaL density values of guanabenz (guan)-induced suppression of I CaL in the absence and presence of the antagonist ARC 239. Horizontal bars denote the protocol of the drug applications. ( c ) ARC 239 induced a minor rightward shift of dose-dependent inhibition of I CaL at the lower concentrations of guanabenz.
    α2b, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 94 stars, based on 1 article reviews
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    1) Product Images from "Role of α2-Adrenoceptor Subtypes in Suppression of L-Type Ca 2+ Current in Mouse Cardiac Myocytes"

    Article Title: Role of α2-Adrenoceptor Subtypes in Suppression of L-Type Ca 2+ Current in Mouse Cardiac Myocytes

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms22084135

    The effect of ARC 239, a specific antagonist of the α2B isoform, on the guanabenz-induced suppression of I CaL . ( a , b ) Representative time-courses of peak I CaL density values of guanabenz (guan)-induced suppression of I CaL in the absence and presence of the antagonist ARC 239. Horizontal bars denote the protocol of the drug applications. ( c ) ARC 239 induced a minor rightward shift of dose-dependent inhibition of I CaL at the lower concentrations of guanabenz.
    Figure Legend Snippet: The effect of ARC 239, a specific antagonist of the α2B isoform, on the guanabenz-induced suppression of I CaL . ( a , b ) Representative time-courses of peak I CaL density values of guanabenz (guan)-induced suppression of I CaL in the absence and presence of the antagonist ARC 239. Horizontal bars denote the protocol of the drug applications. ( c ) ARC 239 induced a minor rightward shift of dose-dependent inhibition of I CaL at the lower concentrations of guanabenz.

    Techniques Used: Inhibition

    The mRNA and protein expression levels of the α2-AR isoforms in the mouse left ventricle. ( a ) mRNA expression of the α2 adrenoceptor genes obtained using the RT-qPCR assay. In contrast to adra2B and adrs2C genes the expression of adra2A was not detected (ND). ( b ) Western blots confirmed the expression of α2B and α2C but not α2A receptor proteins in the mouse left ventricular tissue.
    Figure Legend Snippet: The mRNA and protein expression levels of the α2-AR isoforms in the mouse left ventricle. ( a ) mRNA expression of the α2 adrenoceptor genes obtained using the RT-qPCR assay. In contrast to adra2B and adrs2C genes the expression of adra2A was not detected (ND). ( b ) Western blots confirmed the expression of α2B and α2C but not α2A receptor proteins in the mouse left ventricular tissue.

    Techniques Used: Expressing, Quantitative RT-PCR, Western Blot

    anti adra2b  (Alomone Labs)


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    Alomone Labs anti adra2b
    Violin plots showing distribution (left) and feature plots (right) showing individual cellular mRNA expression of ( A ) Klf1, ( B ) Epor, and ( C ) <t>Adra2b</t> superimposed on the clusters.
    Anti Adra2b, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 94 stars, based on 1 article reviews
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    94/100 stars

    Images

    1) Product Images from "EKLF/KLF1 expression defines a unique macrophage subset during mouse erythropoiesis"

    Article Title: EKLF/KLF1 expression defines a unique macrophage subset during mouse erythropoiesis

    Journal: eLife

    doi: 10.7554/eLife.61070

    Violin plots showing distribution (left) and feature plots (right) showing individual cellular mRNA expression of ( A ) Klf1, ( B ) Epor, and ( C ) Adra2b superimposed on the clusters.
    Figure Legend Snippet: Violin plots showing distribution (left) and feature plots (right) showing individual cellular mRNA expression of ( A ) Klf1, ( B ) Epor, and ( C ) Adra2b superimposed on the clusters.

    Techniques Used: Expressing


    Figure Legend Snippet:

    Techniques Used: Recombinant, Selection, Antibody Labeling, Conjugation Assay, Software

    anti adra2b  (Alomone Labs)


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    Alomone Labs anti adra2b
    Violin plots showing distribution (left) and feature plots (right) showing individual cellular mRNA expression of ( A ) Klf1, ( B ) Epor, and ( C ) <t>Adra2b</t> superimposed on the clusters.
    Anti Adra2b, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti adra2b/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
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    anti adra2b - by Bioz Stars, 2023-02
    94/100 stars

    Images

    1) Product Images from "EKLF/KLF1 expression defines a unique macrophage subset during mouse erythropoiesis"

    Article Title: EKLF/KLF1 expression defines a unique macrophage subset during mouse erythropoiesis

    Journal: eLife

    doi: 10.7554/eLife.61070

    Violin plots showing distribution (left) and feature plots (right) showing individual cellular mRNA expression of ( A ) Klf1, ( B ) Epor, and ( C ) Adra2b superimposed on the clusters.
    Figure Legend Snippet: Violin plots showing distribution (left) and feature plots (right) showing individual cellular mRNA expression of ( A ) Klf1, ( B ) Epor, and ( C ) Adra2b superimposed on the clusters.

    Techniques Used: Expressing


    Figure Legend Snippet:

    Techniques Used: Recombinant, Selection, Antibody Labeling, Conjugation Assay, Software

    α2b adrenergic receptor  (Alomone Labs)


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    Alomone Labs α2b adrenergic receptor
    α2b Adrenergic Receptor, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    α2b adrenergic receptor - by Bioz Stars, 2023-02
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    α2b adrenergic receptor  (Alomone Labs)


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    Alomone Labs α2b adrenergic receptor
    α2b Adrenergic Receptor, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/α2b adrenergic receptor/product/Alomone Labs
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    anti adra2b  (Alomone Labs)


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    Alomone Labs anti adra2b
    (A) Heatmap of log2 FPKM values of F4/80+ FL signature genes that are also enriched in F4/80+ EKLF/GFP+. (B) Heatmap of log2 FPKM and fold changes of FL signature genes that are significantly downregulated in EKLF-/-. Red boxes depict the EKLF dependent signature genes that are common to both (A) and (B). (C) Density plot of flow cytometry analysis of E13.5 fetal livers stained with F4/80 and <t>Adra2b</t> antibodies. Gating scheme for F4/80-hi and Adra2b+ cells is shown in blue. The percentage of double positive Adra2b and F4/80hi cells, compared to total Adra2b cells, is indicated. (D) Representative pictures of erythroblastic islands from E13.5 fetal livers are shown after immunostaining with anti-F4/80 (red) or anti-Adra2b (green) antibodies. DNA stain was with DAPI (blue).
    Anti Adra2b, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti adra2b/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
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    anti adra2b - by Bioz Stars, 2023-02
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    Images

    1) Product Images from "EKLF/KLF1 expression defines a unique macrophage subset during mouse erythropoiesis"

    Article Title: EKLF/KLF1 expression defines a unique macrophage subset during mouse erythropoiesis

    Journal: bioRxiv

    doi: 10.1101/2020.12.23.424143

    (A) Heatmap of log2 FPKM values of F4/80+ FL signature genes that are also enriched in F4/80+ EKLF/GFP+. (B) Heatmap of log2 FPKM and fold changes of FL signature genes that are significantly downregulated in EKLF-/-. Red boxes depict the EKLF dependent signature genes that are common to both (A) and (B). (C) Density plot of flow cytometry analysis of E13.5 fetal livers stained with F4/80 and Adra2b antibodies. Gating scheme for F4/80-hi and Adra2b+ cells is shown in blue. The percentage of double positive Adra2b and F4/80hi cells, compared to total Adra2b cells, is indicated. (D) Representative pictures of erythroblastic islands from E13.5 fetal livers are shown after immunostaining with anti-F4/80 (red) or anti-Adra2b (green) antibodies. DNA stain was with DAPI (blue).
    Figure Legend Snippet: (A) Heatmap of log2 FPKM values of F4/80+ FL signature genes that are also enriched in F4/80+ EKLF/GFP+. (B) Heatmap of log2 FPKM and fold changes of FL signature genes that are significantly downregulated in EKLF-/-. Red boxes depict the EKLF dependent signature genes that are common to both (A) and (B). (C) Density plot of flow cytometry analysis of E13.5 fetal livers stained with F4/80 and Adra2b antibodies. Gating scheme for F4/80-hi and Adra2b+ cells is shown in blue. The percentage of double positive Adra2b and F4/80hi cells, compared to total Adra2b cells, is indicated. (D) Representative pictures of erythroblastic islands from E13.5 fetal livers are shown after immunostaining with anti-F4/80 (red) or anti-Adra2b (green) antibodies. DNA stain was with DAPI (blue).

    Techniques Used: Flow Cytometry, Staining, Immunostaining

    Violin plots showing distribution (left) and Feature plots (right) showing individual cellular mRNA expression of (A) Klf1, (B) Epor, and (C) Adra2b superimposed on the clusters.
    Figure Legend Snippet: Violin plots showing distribution (left) and Feature plots (right) showing individual cellular mRNA expression of (A) Klf1, (B) Epor, and (C) Adra2b superimposed on the clusters.

    Techniques Used: Expressing

    anti α2c  (Alomone Labs)


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    Alomone Labs anti α2c
    α-ARs immunostaining in response to DREADD hM4Di inhibition of the LC. For all α-ARs, the main effects were attributable to treatment since compensatory receptor expressions were seen in (A) Regions examined by α-AR IF. Representative immunofluorescence for α1a-AR comparing all cohorts (parietal region) (B). Normalized fluorescent intensities for (C) α1a-AR (F1,56 = 9.45, p = .003), (D) α2a-AR (F1,56 = 17.2, p = .0001), (E) α2b-AR (F1,44 = 43.91, p < .0001) and (F) <t>α2c-AR</t> (F1,44 = 26.40, p < .0001). A significant effect could also be attributed to karyotype in α1a-AR (F1,56 = 8.40, p = .005), and α2c-AR (F1,44 = 17.73, p = .0001). Scale bar = 100 μm. (G) Expression levels of α2a-, α2b- and α2c-ARs negatively correlated with 24-h NORT DI performance. Error bars represent the mean ± SEM. Dotted lines represented the 95% confidence interval.
    Anti α2c, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "Inhibitory designer receptors aggravate memory loss in a mouse model of down syndrome"

    Article Title: Inhibitory designer receptors aggravate memory loss in a mouse model of down syndrome

    Journal: Neurobiology of disease

    doi: 10.1016/j.nbd.2019.104616

    α-ARs immunostaining in response to DREADD hM4Di inhibition of the LC. For all α-ARs, the main effects were attributable to treatment since compensatory receptor expressions were seen in (A) Regions examined by α-AR IF. Representative immunofluorescence for α1a-AR comparing all cohorts (parietal region) (B). Normalized fluorescent intensities for (C) α1a-AR (F1,56 = 9.45, p = .003), (D) α2a-AR (F1,56 = 17.2, p = .0001), (E) α2b-AR (F1,44 = 43.91, p < .0001) and (F) α2c-AR (F1,44 = 26.40, p < .0001). A significant effect could also be attributed to karyotype in α1a-AR (F1,56 = 8.40, p = .005), and α2c-AR (F1,44 = 17.73, p = .0001). Scale bar = 100 μm. (G) Expression levels of α2a-, α2b- and α2c-ARs negatively correlated with 24-h NORT DI performance. Error bars represent the mean ± SEM. Dotted lines represented the 95% confidence interval.
    Figure Legend Snippet: α-ARs immunostaining in response to DREADD hM4Di inhibition of the LC. For all α-ARs, the main effects were attributable to treatment since compensatory receptor expressions were seen in (A) Regions examined by α-AR IF. Representative immunofluorescence for α1a-AR comparing all cohorts (parietal region) (B). Normalized fluorescent intensities for (C) α1a-AR (F1,56 = 9.45, p = .003), (D) α2a-AR (F1,56 = 17.2, p = .0001), (E) α2b-AR (F1,44 = 43.91, p < .0001) and (F) α2c-AR (F1,44 = 26.40, p < .0001). A significant effect could also be attributed to karyotype in α1a-AR (F1,56 = 8.40, p = .005), and α2c-AR (F1,44 = 17.73, p = .0001). Scale bar = 100 μm. (G) Expression levels of α2a-, α2b- and α2c-ARs negatively correlated with 24-h NORT DI performance. Error bars represent the mean ± SEM. Dotted lines represented the 95% confidence interval.

    Techniques Used: Immunostaining, Inhibition, Immunofluorescence, Expressing

    2b ar  (Alomone Labs)


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    Alomone Labs 2b ar
    Adenosine concentrations were measured in BAL from four SD control rats or from isolated left and right lungs from four SD rats collected 24 hrs after left pulmonary artery ligation. <t>Fig</t> <t>2a</t> shows representative chromatograms from an adenosine standard (3µM) and from BAL collected from the right and left lungs of ischemic/reperfused rats. The graph in <t>2b</t> shows individual values for samples. The concentration of adenosine was increased more than 4-fold in BAL from ischemic lungs versus contralateral non-ischemic lungs (*p<0.008). Horizontal lines represent median values for each of the three groups.
    2b Ar, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Lung Injury Pathways: Adenosine Receptor 2B Signaling Limits Development of Ischemic Bronchiolitis Obliterans Organizing Pneumonia"

    Article Title: Lung Injury Pathways: Adenosine Receptor 2B Signaling Limits Development of Ischemic Bronchiolitis Obliterans Organizing Pneumonia

    Journal: Experimental lung research

    doi: 10.1080/01902148.2017.1286697

    Adenosine concentrations were measured in BAL from four SD control rats or from isolated left and right lungs from four SD rats collected 24 hrs after left pulmonary artery ligation. Fig 2a shows representative chromatograms from an adenosine standard (3µM) and from BAL collected from the right and left lungs of ischemic/reperfused rats. The graph in 2b shows individual values for samples. The concentration of adenosine was increased more than 4-fold in BAL from ischemic lungs versus contralateral non-ischemic lungs (*p<0.008). Horizontal lines represent median values for each of the three groups.
    Figure Legend Snippet: Adenosine concentrations were measured in BAL from four SD control rats or from isolated left and right lungs from four SD rats collected 24 hrs after left pulmonary artery ligation. Fig 2a shows representative chromatograms from an adenosine standard (3µM) and from BAL collected from the right and left lungs of ischemic/reperfused rats. The graph in 2b shows individual values for samples. The concentration of adenosine was increased more than 4-fold in BAL from ischemic lungs versus contralateral non-ischemic lungs (*p<0.008). Horizontal lines represent median values for each of the three groups.

    Techniques Used: Isolation, Ligation, Concentration Assay

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    Alomone Labs rabbit anti a 2b ar
    Rabbit Anti A 2b Ar, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Alomone Labs anti α2b adrenoreceptor
    Anti α2b Adrenoreceptor, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Alomone Labs α2b
    The effect of ARC 239, a specific antagonist of the <t>α2B</t> isoform, on the guanabenz-induced suppression of I CaL . ( a , b ) Representative time-courses of peak I CaL density values of guanabenz (guan)-induced suppression of I CaL in the absence and presence of the antagonist ARC 239. Horizontal bars denote the protocol of the drug applications. ( c ) ARC 239 induced a minor rightward shift of dose-dependent inhibition of I CaL at the lower concentrations of guanabenz.
    α2b, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Alomone Labs anti adra2b
    Violin plots showing distribution (left) and feature plots (right) showing individual cellular mRNA expression of ( A ) Klf1, ( B ) Epor, and ( C ) <t>Adra2b</t> superimposed on the clusters.
    Anti Adra2b, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Alomone Labs α2b adrenergic receptor
    Violin plots showing distribution (left) and feature plots (right) showing individual cellular mRNA expression of ( A ) Klf1, ( B ) Epor, and ( C ) <t>Adra2b</t> superimposed on the clusters.
    α2b Adrenergic Receptor, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Alomone Labs anti α2c
    α-ARs immunostaining in response to DREADD hM4Di inhibition of the LC. For all α-ARs, the main effects were attributable to treatment since compensatory receptor expressions were seen in (A) Regions examined by α-AR IF. Representative immunofluorescence for α1a-AR comparing all cohorts (parietal region) (B). Normalized fluorescent intensities for (C) α1a-AR (F1,56 = 9.45, p = .003), (D) α2a-AR (F1,56 = 17.2, p = .0001), (E) α2b-AR (F1,44 = 43.91, p < .0001) and (F) <t>α2c-AR</t> (F1,44 = 26.40, p < .0001). A significant effect could also be attributed to karyotype in α1a-AR (F1,56 = 8.40, p = .005), and α2c-AR (F1,44 = 17.73, p = .0001). Scale bar = 100 μm. (G) Expression levels of α2a-, α2b- and α2c-ARs negatively correlated with 24-h NORT DI performance. Error bars represent the mean ± SEM. Dotted lines represented the 95% confidence interval.
    Anti α2c, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Alomone Labs 2b ar
    Adenosine concentrations were measured in BAL from four SD control rats or from isolated left and right lungs from four SD rats collected 24 hrs after left pulmonary artery ligation. <t>Fig</t> <t>2a</t> shows representative chromatograms from an adenosine standard (3µM) and from BAL collected from the right and left lungs of ischemic/reperfused rats. The graph in <t>2b</t> shows individual values for samples. The concentration of adenosine was increased more than 4-fold in BAL from ischemic lungs versus contralateral non-ischemic lungs (*p<0.008). Horizontal lines represent median values for each of the three groups.
    2b Ar, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    The effect of ARC 239, a specific antagonist of the α2B isoform, on the guanabenz-induced suppression of I CaL . ( a , b ) Representative time-courses of peak I CaL density values of guanabenz (guan)-induced suppression of I CaL in the absence and presence of the antagonist ARC 239. Horizontal bars denote the protocol of the drug applications. ( c ) ARC 239 induced a minor rightward shift of dose-dependent inhibition of I CaL at the lower concentrations of guanabenz.

    Journal: International Journal of Molecular Sciences

    Article Title: Role of α2-Adrenoceptor Subtypes in Suppression of L-Type Ca 2+ Current in Mouse Cardiac Myocytes

    doi: 10.3390/ijms22084135

    Figure Lengend Snippet: The effect of ARC 239, a specific antagonist of the α2B isoform, on the guanabenz-induced suppression of I CaL . ( a , b ) Representative time-courses of peak I CaL density values of guanabenz (guan)-induced suppression of I CaL in the absence and presence of the antagonist ARC 239. Horizontal bars denote the protocol of the drug applications. ( c ) ARC 239 induced a minor rightward shift of dose-dependent inhibition of I CaL at the lower concentrations of guanabenz.

    Article Snippet: Proteins were separated in 10% SDS-PAGE, transferred to nitrocellulose membranes (sc-3724, 0.45 µm, Santa Cruz Biotechnology) and probed with antibodies against α2A (AAR-020, Alomone labs, lot AAR020AN0202), α2B (AAR-021, Alomone labs, lot AAR021AN0202) and α2C (ab46536, Abcam) diluted to 1:100.

    Techniques: Inhibition

    The mRNA and protein expression levels of the α2-AR isoforms in the mouse left ventricle. ( a ) mRNA expression of the α2 adrenoceptor genes obtained using the RT-qPCR assay. In contrast to adra2B and adrs2C genes the expression of adra2A was not detected (ND). ( b ) Western blots confirmed the expression of α2B and α2C but not α2A receptor proteins in the mouse left ventricular tissue.

    Journal: International Journal of Molecular Sciences

    Article Title: Role of α2-Adrenoceptor Subtypes in Suppression of L-Type Ca 2+ Current in Mouse Cardiac Myocytes

    doi: 10.3390/ijms22084135

    Figure Lengend Snippet: The mRNA and protein expression levels of the α2-AR isoforms in the mouse left ventricle. ( a ) mRNA expression of the α2 adrenoceptor genes obtained using the RT-qPCR assay. In contrast to adra2B and adrs2C genes the expression of adra2A was not detected (ND). ( b ) Western blots confirmed the expression of α2B and α2C but not α2A receptor proteins in the mouse left ventricular tissue.

    Article Snippet: Proteins were separated in 10% SDS-PAGE, transferred to nitrocellulose membranes (sc-3724, 0.45 µm, Santa Cruz Biotechnology) and probed with antibodies against α2A (AAR-020, Alomone labs, lot AAR020AN0202), α2B (AAR-021, Alomone labs, lot AAR021AN0202) and α2C (ab46536, Abcam) diluted to 1:100.

    Techniques: Expressing, Quantitative RT-PCR, Western Blot

    Violin plots showing distribution (left) and feature plots (right) showing individual cellular mRNA expression of ( A ) Klf1, ( B ) Epor, and ( C ) Adra2b superimposed on the clusters.

    Journal: eLife

    Article Title: EKLF/KLF1 expression defines a unique macrophage subset during mouse erythropoiesis

    doi: 10.7554/eLife.61070

    Figure Lengend Snippet: Violin plots showing distribution (left) and feature plots (right) showing individual cellular mRNA expression of ( A ) Klf1, ( B ) Epor, and ( C ) Adra2b superimposed on the clusters.

    Article Snippet: Suspended cells from FLs were stained for FACS with the following antibodies: anti-mouse F4/80-PE (eBiosciences #12-4801-80), anti-Adra2b (Alomone Labs #AAR-021), anti-adducinβ (Santa Cruz # sc-376063), and anti-spectrinβ1 (Santa Cruz # sc-374309).

    Techniques: Expressing

    Journal: eLife

    Article Title: EKLF/KLF1 expression defines a unique macrophage subset during mouse erythropoiesis

    doi: 10.7554/eLife.61070

    Figure Lengend Snippet:

    Article Snippet: Suspended cells from FLs were stained for FACS with the following antibodies: anti-mouse F4/80-PE (eBiosciences #12-4801-80), anti-Adra2b (Alomone Labs #AAR-021), anti-adducinβ (Santa Cruz # sc-376063), and anti-spectrinβ1 (Santa Cruz # sc-374309).

    Techniques: Recombinant, Selection, Antibody Labeling, Conjugation Assay, Software

    α-ARs immunostaining in response to DREADD hM4Di inhibition of the LC. For all α-ARs, the main effects were attributable to treatment since compensatory receptor expressions were seen in (A) Regions examined by α-AR IF. Representative immunofluorescence for α1a-AR comparing all cohorts (parietal region) (B). Normalized fluorescent intensities for (C) α1a-AR (F1,56 = 9.45, p = .003), (D) α2a-AR (F1,56 = 17.2, p = .0001), (E) α2b-AR (F1,44 = 43.91, p < .0001) and (F) α2c-AR (F1,44 = 26.40, p < .0001). A significant effect could also be attributed to karyotype in α1a-AR (F1,56 = 8.40, p = .005), and α2c-AR (F1,44 = 17.73, p = .0001). Scale bar = 100 μm. (G) Expression levels of α2a-, α2b- and α2c-ARs negatively correlated with 24-h NORT DI performance. Error bars represent the mean ± SEM. Dotted lines represented the 95% confidence interval.

    Journal: Neurobiology of disease

    Article Title: Inhibitory designer receptors aggravate memory loss in a mouse model of down syndrome

    doi: 10.1016/j.nbd.2019.104616

    Figure Lengend Snippet: α-ARs immunostaining in response to DREADD hM4Di inhibition of the LC. For all α-ARs, the main effects were attributable to treatment since compensatory receptor expressions were seen in (A) Regions examined by α-AR IF. Representative immunofluorescence for α1a-AR comparing all cohorts (parietal region) (B). Normalized fluorescent intensities for (C) α1a-AR (F1,56 = 9.45, p = .003), (D) α2a-AR (F1,56 = 17.2, p = .0001), (E) α2b-AR (F1,44 = 43.91, p < .0001) and (F) α2c-AR (F1,44 = 26.40, p < .0001). A significant effect could also be attributed to karyotype in α1a-AR (F1,56 = 8.40, p = .005), and α2c-AR (F1,44 = 17.73, p = .0001). Scale bar = 100 μm. (G) Expression levels of α2a-, α2b- and α2c-ARs negatively correlated with 24-h NORT DI performance. Error bars represent the mean ± SEM. Dotted lines represented the 95% confidence interval.

    Article Snippet: For ARs, sections were incubated overnight at room temperature with anti-β1 (#AAR-023, dil. 1:400), anti-β2 (#AAR-016, dil. 1:200), anti-β3 (#AAR-017, dil. 1:100), anti-α1a (#AAR-015, dil. 1:100), anti-α2a (#AAR-020, dil. 1:200), anti-α2b (#AAR-021, dil. 1:100), or anti-α2c (#AAR-021, dil. 1:100) AR antibodies with or without blocking peptides at the recommended concentrations (Alomone Labs, Jerusalem, Israel).

    Techniques: Immunostaining, Inhibition, Immunofluorescence, Expressing

    Adenosine concentrations were measured in BAL from four SD control rats or from isolated left and right lungs from four SD rats collected 24 hrs after left pulmonary artery ligation. Fig 2a shows representative chromatograms from an adenosine standard (3µM) and from BAL collected from the right and left lungs of ischemic/reperfused rats. The graph in 2b shows individual values for samples. The concentration of adenosine was increased more than 4-fold in BAL from ischemic lungs versus contralateral non-ischemic lungs (*p<0.008). Horizontal lines represent median values for each of the three groups.

    Journal: Experimental lung research

    Article Title: Lung Injury Pathways: Adenosine Receptor 2B Signaling Limits Development of Ischemic Bronchiolitis Obliterans Organizing Pneumonia

    doi: 10.1080/01902148.2017.1286697

    Figure Lengend Snippet: Adenosine concentrations were measured in BAL from four SD control rats or from isolated left and right lungs from four SD rats collected 24 hrs after left pulmonary artery ligation. Fig 2a shows representative chromatograms from an adenosine standard (3µM) and from BAL collected from the right and left lungs of ischemic/reperfused rats. The graph in 2b shows individual values for samples. The concentration of adenosine was increased more than 4-fold in BAL from ischemic lungs versus contralateral non-ischemic lungs (*p<0.008). Horizontal lines represent median values for each of the three groups.

    Article Snippet: Antibodies for A 2A AR and A 2B AR were purchased from Alomone labs (A 2B AR Cat# AAR-003, A 2A AR Cat# AAR-002).

    Techniques: Isolation, Ligation, Concentration Assay