Journal: International Journal of Molecular Sciences

Article Title: Role of α2-Adrenoceptor Subtypes in Suppression of L-Type Ca 2+ Current in Mouse Cardiac Myocytes

doi: 10.3390/ijms22084135

Figure Lengend Snippet: The effect of ARC 239, a specific antagonist of the α2B isoform, on the guanabenz-induced suppression of I CaL . ( a , b ) Representative time-courses of peak I CaL density values of guanabenz (guan)-induced suppression of I CaL in the absence and presence of the antagonist ARC 239. Horizontal bars denote the protocol of the drug applications. ( c ) ARC 239 induced a minor rightward shift of dose-dependent inhibition of I CaL at the lower concentrations of guanabenz.

Article Snippet: Proteins were separated in 10% SDS-PAGE, transferred to nitrocellulose membranes (sc-3724, 0.45 µm, Santa Cruz Biotechnology) and probed with antibodies against α2A (AAR-020, Alomone labs, lot AAR020AN0202), α2B (AAR-021, Alomone labs, lot AAR021AN0202) and α2C (ab46536, Abcam) diluted to 1:100.

Techniques: Inhibition

Journal: International Journal of Molecular Sciences

Article Title: Role of α2-Adrenoceptor Subtypes in Suppression of L-Type Ca 2+ Current in Mouse Cardiac Myocytes

doi: 10.3390/ijms22084135

Figure Lengend Snippet: The mRNA and protein expression levels of the α2-AR isoforms in the mouse left ventricle. ( a ) mRNA expression of the α2 adrenoceptor genes obtained using the RT-qPCR assay. In contrast to adra2B and adrs2C genes the expression of adra2A was not detected (ND). ( b ) Western blots confirmed the expression of α2B and α2C but not α2A receptor proteins in the mouse left ventricular tissue.

Article Snippet: Proteins were separated in 10% SDS-PAGE, transferred to nitrocellulose membranes (sc-3724, 0.45 µm, Santa Cruz Biotechnology) and probed with antibodies against α2A (AAR-020, Alomone labs, lot AAR020AN0202), α2B (AAR-021, Alomone labs, lot AAR021AN0202) and α2C (ab46536, Abcam) diluted to 1:100.

Techniques: Expressing, Quantitative RT-PCR, Western Blot

Journal: eLife

Article Title: EKLF/KLF1 expression defines a unique macrophage subset during mouse erythropoiesis

doi: 10.7554/eLife.61070

Figure Lengend Snippet: Violin plots showing distribution (left) and feature plots (right) showing individual cellular mRNA expression of ( A ) Klf1, ( B ) Epor, and ( C ) Adra2b superimposed on the clusters.

Article Snippet: Suspended cells from FLs were stained for FACS with the following antibodies: anti-mouse F4/80-PE (eBiosciences #12-4801-80), anti-Adra2b (Alomone Labs #AAR-021), anti-adducinβ (Santa Cruz # sc-376063), and anti-spectrinβ1 (Santa Cruz # sc-374309).

Techniques: Expressing

Journal: eLife

Article Title: EKLF/KLF1 expression defines a unique macrophage subset during mouse erythropoiesis

doi: 10.7554/eLife.61070

Figure Lengend Snippet:

Article Snippet: Suspended cells from FLs were stained for FACS with the following antibodies: anti-mouse F4/80-PE (eBiosciences #12-4801-80), anti-Adra2b (Alomone Labs #AAR-021), anti-adducinβ (Santa Cruz # sc-376063), and anti-spectrinβ1 (Santa Cruz # sc-374309).

Techniques: Recombinant, Selection, Antibody Labeling, Conjugation Assay, Software

Journal: Neurobiology of disease

Article Title: Inhibitory designer receptors aggravate memory loss in a mouse model of down syndrome

doi: 10.1016/j.nbd.2019.104616

Figure Lengend Snippet: α-ARs immunostaining in response to DREADD hM4Di inhibition of the LC. For all α-ARs, the main effects were attributable to treatment since compensatory receptor expressions were seen in (A) Regions examined by α-AR IF. Representative immunofluorescence for α1a-AR comparing all cohorts (parietal region) (B). Normalized fluorescent intensities for (C) α1a-AR (F1,56 = 9.45, p = .003), (D) α2a-AR (F1,56 = 17.2, p = .0001), (E) α2b-AR (F1,44 = 43.91, p < .0001) and (F) α2c-AR (F1,44 = 26.40, p < .0001). A significant effect could also be attributed to karyotype in α1a-AR (F1,56 = 8.40, p = .005), and α2c-AR (F1,44 = 17.73, p = .0001). Scale bar = 100 μm. (G) Expression levels of α2a-, α2b- and α2c-ARs negatively correlated with 24-h NORT DI performance. Error bars represent the mean ± SEM. Dotted lines represented the 95% confidence interval.

Article Snippet: For ARs, sections were incubated overnight at room temperature with anti-β1 (#AAR-023, dil. 1:400), anti-β2 (#AAR-016, dil. 1:200), anti-β3 (#AAR-017, dil. 1:100), anti-α1a (#AAR-015, dil. 1:100), anti-α2a (#AAR-020, dil. 1:200), anti-α2b (#AAR-021, dil. 1:100), or anti-α2c (#AAR-021, dil. 1:100) AR antibodies with or without blocking peptides at the recommended concentrations (Alomone Labs, Jerusalem, Israel).

Techniques: Immunostaining, Inhibition, Immunofluorescence, Expressing

Journal: Experimental lung research

Article Title: Lung Injury Pathways: Adenosine Receptor 2B Signaling Limits Development of Ischemic Bronchiolitis Obliterans Organizing Pneumonia

doi: 10.1080/01902148.2017.1286697

Figure Lengend Snippet: Adenosine concentrations were measured in BAL from four SD control rats or from isolated left and right lungs from four SD rats collected 24 hrs after left pulmonary artery ligation. Fig 2a shows representative chromatograms from an adenosine standard (3µM) and from BAL collected from the right and left lungs of ischemic/reperfused rats. The graph in 2b shows individual values for samples. The concentration of adenosine was increased more than 4-fold in BAL from ischemic lungs versus contralateral non-ischemic lungs (*p<0.008). Horizontal lines represent median values for each of the three groups.

Article Snippet: Antibodies for A 2A AR and A 2B AR were purchased from Alomone labs (A 2B AR Cat# AAR-003, A 2A AR Cat# AAR-002).

Techniques: Isolation, Ligation, Concentration Assay