anti bk α subunit antibody  (Alomone Labs)


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    Structured Review

    Alomone Labs anti bk α subunit antibody
    Expression of BK α and β2 in WT SCNs. ( a ) BK channel complexes were immunoprecipitated with an anti-BK <t>α</t> subunit antibody from WT SCNs at the indicated time points. Western blot analysis was performed for BK α (top panel), β2 (middle) or α-tubulin (bottom). α-Tubulin westerns (loading control) were obtained by running an equivalent volume of supernatant as was used for the immunoprecipitation. Protein was also harvested from α+β2 subunits co-expressed in HEK293 cells (positive control) or from β2 KO SCNs (negative control). ZT, zeitgeber time. Images have been cropped for presentation. Full size images are presented in Supplementary Fig. 3 . ( b ) BK α and β2 band intensities normalized to α-tubulin. α expression increases at night, while β2 expression does not change. Data are the average from four independent timed SCN collections (four SCNs at each timepoint). ( c ) Ratio of β2:α expression in WT SCNs across the circadian cycle. All values are mean±s.e.m. * P
    Anti Bk α Subunit Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti bk α subunit antibody/product/Alomone Labs
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti bk α subunit antibody - by Bioz Stars, 2022-12
    92/100 stars

    Images

    1) Product Images from "BK channel inactivation gates daytime excitability in the circadian clock"

    Article Title: BK channel inactivation gates daytime excitability in the circadian clock

    Journal: Nature Communications

    doi: 10.1038/ncomms10837

    Expression of BK α and β2 in WT SCNs. ( a ) BK channel complexes were immunoprecipitated with an anti-BK α subunit antibody from WT SCNs at the indicated time points. Western blot analysis was performed for BK α (top panel), β2 (middle) or α-tubulin (bottom). α-Tubulin westerns (loading control) were obtained by running an equivalent volume of supernatant as was used for the immunoprecipitation. Protein was also harvested from α+β2 subunits co-expressed in HEK293 cells (positive control) or from β2 KO SCNs (negative control). ZT, zeitgeber time. Images have been cropped for presentation. Full size images are presented in Supplementary Fig. 3 . ( b ) BK α and β2 band intensities normalized to α-tubulin. α expression increases at night, while β2 expression does not change. Data are the average from four independent timed SCN collections (four SCNs at each timepoint). ( c ) Ratio of β2:α expression in WT SCNs across the circadian cycle. All values are mean±s.e.m. * P
    Figure Legend Snippet: Expression of BK α and β2 in WT SCNs. ( a ) BK channel complexes were immunoprecipitated with an anti-BK α subunit antibody from WT SCNs at the indicated time points. Western blot analysis was performed for BK α (top panel), β2 (middle) or α-tubulin (bottom). α-Tubulin westerns (loading control) were obtained by running an equivalent volume of supernatant as was used for the immunoprecipitation. Protein was also harvested from α+β2 subunits co-expressed in HEK293 cells (positive control) or from β2 KO SCNs (negative control). ZT, zeitgeber time. Images have been cropped for presentation. Full size images are presented in Supplementary Fig. 3 . ( b ) BK α and β2 band intensities normalized to α-tubulin. α expression increases at night, while β2 expression does not change. Data are the average from four independent timed SCN collections (four SCNs at each timepoint). ( c ) Ratio of β2:α expression in WT SCNs across the circadian cycle. All values are mean±s.e.m. * P

    Techniques Used: Expressing, Immunoprecipitation, Western Blot, Positive Control, Negative Control

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    Alomone Labs anti alpha 2 na k atpase antibody
    Expression of BK α and β2 in WT SCNs. ( a ) BK channel complexes were immunoprecipitated with an anti-BK <t>α</t> subunit antibody from WT SCNs at the indicated time points. Western blot analysis was performed for BK α (top panel), β2 (middle) or α-tubulin (bottom). α-Tubulin westerns (loading control) were obtained by running an equivalent volume of supernatant as was used for the immunoprecipitation. Protein was also harvested from α+β2 subunits co-expressed in HEK293 cells (positive control) or from β2 KO SCNs (negative control). ZT, zeitgeber time. Images have been cropped for presentation. Full size images are presented in Supplementary Fig. 3 . ( b ) BK α and β2 band intensities normalized to α-tubulin. α expression increases at night, while β2 expression does not change. Data are the average from four independent timed SCN collections (four SCNs at each timepoint). ( c ) Ratio of β2:α expression in WT SCNs across the circadian cycle. All values are mean±s.e.m. * P
    Anti Alpha 2 Na K Atpase Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 92/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti alpha 2 na k atpase antibody/product/Alomone Labs
    Average 92 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    anti alpha 2 na k atpase antibody - by Bioz Stars, 2022-12
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    94
    Alomone Labs rabbit anti α1 na k atpase
    Elevated pressure decreases expression of total and <t>α1</t> <t>Na/K-ATPase</t> in RGCs in vitro . (A) Representative fluorescent micrographs of total and α1 Na/K-ATPase (green) and β-Tubulin (red) immunolabeling in primary cultures of purified RGCs exposed to ambient or elevated pressure for 48 h. Scale bar = 40 μm. (B) Representative fluorescent micrographs of total and α1 Na/K-ATPase (green) and β-Tubulin (red) immunolabeling in primary cultures of purified RGCs exposed to ambient or elevated pressure for 4 h. Scale bar = 40 μm. (C) Mean intensity (arbitrary units; AU) of total (left) and α1 (right) Na/K-ATPase staining in RGC cultures following a 48-h exposure to ambient or elevated hydrostatic pressure. ∗ p
    Rabbit Anti α1 Na K Atpase, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti α1 na k atpase/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti α1 na k atpase - by Bioz Stars, 2022-12
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    Image Search Results


    Expression of BK α and β2 in WT SCNs. ( a ) BK channel complexes were immunoprecipitated with an anti-BK α subunit antibody from WT SCNs at the indicated time points. Western blot analysis was performed for BK α (top panel), β2 (middle) or α-tubulin (bottom). α-Tubulin westerns (loading control) were obtained by running an equivalent volume of supernatant as was used for the immunoprecipitation. Protein was also harvested from α+β2 subunits co-expressed in HEK293 cells (positive control) or from β2 KO SCNs (negative control). ZT, zeitgeber time. Images have been cropped for presentation. Full size images are presented in Supplementary Fig. 3 . ( b ) BK α and β2 band intensities normalized to α-tubulin. α expression increases at night, while β2 expression does not change. Data are the average from four independent timed SCN collections (four SCNs at each timepoint). ( c ) Ratio of β2:α expression in WT SCNs across the circadian cycle. All values are mean±s.e.m. * P

    Journal: Nature Communications

    Article Title: BK channel inactivation gates daytime excitability in the circadian clock

    doi: 10.1038/ncomms10837

    Figure Lengend Snippet: Expression of BK α and β2 in WT SCNs. ( a ) BK channel complexes were immunoprecipitated with an anti-BK α subunit antibody from WT SCNs at the indicated time points. Western blot analysis was performed for BK α (top panel), β2 (middle) or α-tubulin (bottom). α-Tubulin westerns (loading control) were obtained by running an equivalent volume of supernatant as was used for the immunoprecipitation. Protein was also harvested from α+β2 subunits co-expressed in HEK293 cells (positive control) or from β2 KO SCNs (negative control). ZT, zeitgeber time. Images have been cropped for presentation. Full size images are presented in Supplementary Fig. 3 . ( b ) BK α and β2 band intensities normalized to α-tubulin. α expression increases at night, while β2 expression does not change. Data are the average from four independent timed SCN collections (four SCNs at each timepoint). ( c ) Ratio of β2:α expression in WT SCNs across the circadian cycle. All values are mean±s.e.m. * P

    Article Snippet: Supernatant was collected and mixed with 1 μg of anti-BK α subunit antibody (anti-KCa1.1, #1184–1200, Alamone Labs; Jerusalem, Israel) and rotated for 2 h at 4 °C.

    Techniques: Expressing, Immunoprecipitation, Western Blot, Positive Control, Negative Control

    Elevated pressure decreases expression of total and α1 Na/K-ATPase in RGCs in vitro . (A) Representative fluorescent micrographs of total and α1 Na/K-ATPase (green) and β-Tubulin (red) immunolabeling in primary cultures of purified RGCs exposed to ambient or elevated pressure for 48 h. Scale bar = 40 μm. (B) Representative fluorescent micrographs of total and α1 Na/K-ATPase (green) and β-Tubulin (red) immunolabeling in primary cultures of purified RGCs exposed to ambient or elevated pressure for 4 h. Scale bar = 40 μm. (C) Mean intensity (arbitrary units; AU) of total (left) and α1 (right) Na/K-ATPase staining in RGC cultures following a 48-h exposure to ambient or elevated hydrostatic pressure. ∗ p

    Journal: Frontiers in Neuroscience

    Article Title: Impairment of Membrane Repolarization Accompanies Axon Transport Deficits in Glaucoma

    doi: 10.3389/fnins.2019.01139

    Figure Lengend Snippet: Elevated pressure decreases expression of total and α1 Na/K-ATPase in RGCs in vitro . (A) Representative fluorescent micrographs of total and α1 Na/K-ATPase (green) and β-Tubulin (red) immunolabeling in primary cultures of purified RGCs exposed to ambient or elevated pressure for 48 h. Scale bar = 40 μm. (B) Representative fluorescent micrographs of total and α1 Na/K-ATPase (green) and β-Tubulin (red) immunolabeling in primary cultures of purified RGCs exposed to ambient or elevated pressure for 4 h. Scale bar = 40 μm. (C) Mean intensity (arbitrary units; AU) of total (left) and α1 (right) Na/K-ATPase staining in RGC cultures following a 48-h exposure to ambient or elevated hydrostatic pressure. ∗ p

    Article Snippet: Samples were then incubated overnight at 4°C in primary antibody solution (3% NHS and 0.1% Triton X-100 diluted in 1 × PBS) containing rabbit anti-Total Na/K-ATPase (2 μg/ml, Cat# ab58475, Abcam, Cambridge, United Kingdom), rabbit anti-α1 Na/K-ATPase (2.85 μg/ml, Cat# ANP-001, Alomone, Jerusalem, Israel); and mouse anti-β-Tubulin III (2 μg/ml, Cat# 801201, BioLegend, London, United Kingdom).

    Techniques: Expressing, In Vitro, Immunolabeling, Purification, Staining

    Elevated IOP decreases expression of total and α1 Na/K-ATPase in retina. (A) Box plots of fold change (log scale; left) and percent change (right) in expression of the Atp1 gene family between retina from saline- and microbead-injected mice, as determined by RNA sequencing. n (saline) = 5 retinas, n (microbead) = 5; ∗ p

    Journal: Frontiers in Neuroscience

    Article Title: Impairment of Membrane Repolarization Accompanies Axon Transport Deficits in Glaucoma

    doi: 10.3389/fnins.2019.01139

    Figure Lengend Snippet: Elevated IOP decreases expression of total and α1 Na/K-ATPase in retina. (A) Box plots of fold change (log scale; left) and percent change (right) in expression of the Atp1 gene family between retina from saline- and microbead-injected mice, as determined by RNA sequencing. n (saline) = 5 retinas, n (microbead) = 5; ∗ p

    Article Snippet: Samples were then incubated overnight at 4°C in primary antibody solution (3% NHS and 0.1% Triton X-100 diluted in 1 × PBS) containing rabbit anti-Total Na/K-ATPase (2 μg/ml, Cat# ab58475, Abcam, Cambridge, United Kingdom), rabbit anti-α1 Na/K-ATPase (2.85 μg/ml, Cat# ANP-001, Alomone, Jerusalem, Israel); and mouse anti-β-Tubulin III (2 μg/ml, Cat# 801201, BioLegend, London, United Kingdom).

    Techniques: Expressing, Injection, Mouse Assay, RNA Sequencing Assay

    Inhibition of endocytosis and degradation pathways preserves Na/K-ATPase expression in RGCs following short-term pressure exposure. (A) Representative confocal micrographs of total Na/K-ATPase (green) and β-Tubulin III (red) immunolabeling in primary, purified RGCs treated with vehicle, 10 μM bisindolylmaleimide, or 20 μM MG-132 and exposed to ambient or elevated pressure for 4 h. Scale bar = 40 μM. (B) Mean intensity (arbitrary units; AU) of total Na/K-ATPase staining per cell in each drug and pressure condition. ∗ p

    Journal: Frontiers in Neuroscience

    Article Title: Impairment of Membrane Repolarization Accompanies Axon Transport Deficits in Glaucoma

    doi: 10.3389/fnins.2019.01139

    Figure Lengend Snippet: Inhibition of endocytosis and degradation pathways preserves Na/K-ATPase expression in RGCs following short-term pressure exposure. (A) Representative confocal micrographs of total Na/K-ATPase (green) and β-Tubulin III (red) immunolabeling in primary, purified RGCs treated with vehicle, 10 μM bisindolylmaleimide, or 20 μM MG-132 and exposed to ambient or elevated pressure for 4 h. Scale bar = 40 μM. (B) Mean intensity (arbitrary units; AU) of total Na/K-ATPase staining per cell in each drug and pressure condition. ∗ p

    Article Snippet: Samples were then incubated overnight at 4°C in primary antibody solution (3% NHS and 0.1% Triton X-100 diluted in 1 × PBS) containing rabbit anti-Total Na/K-ATPase (2 μg/ml, Cat# ab58475, Abcam, Cambridge, United Kingdom), rabbit anti-α1 Na/K-ATPase (2.85 μg/ml, Cat# ANP-001, Alomone, Jerusalem, Israel); and mouse anti-β-Tubulin III (2 μg/ml, Cat# 801201, BioLegend, London, United Kingdom).

    Techniques: Inhibition, Expressing, Immunolabeling, Purification, Staining

    Inhibition of Na/K-ATPase endocytosis and degradation prevents pressure-induced reduction of inward cation flux. (A) Representative heat maps showing the fluorescent signal of Thallos dye in RGCs exposed to ambient and elevated pressure for 4 h plus treatment with vehicle, 10 μM bisindolylmaleimide, or 20 μM MG-132. Images were taken after the addition of thallium. Scale bar = 100 μM. (B) Line graph displaying the mean fluorescence intensity of Thallos dyes over time for RGCs exposed to ambient or elevated pressure for 4 h plus treatment with vehicle or bisindolylmaleimide. ∗ p

    Journal: Frontiers in Neuroscience

    Article Title: Impairment of Membrane Repolarization Accompanies Axon Transport Deficits in Glaucoma

    doi: 10.3389/fnins.2019.01139

    Figure Lengend Snippet: Inhibition of Na/K-ATPase endocytosis and degradation prevents pressure-induced reduction of inward cation flux. (A) Representative heat maps showing the fluorescent signal of Thallos dye in RGCs exposed to ambient and elevated pressure for 4 h plus treatment with vehicle, 10 μM bisindolylmaleimide, or 20 μM MG-132. Images were taken after the addition of thallium. Scale bar = 100 μM. (B) Line graph displaying the mean fluorescence intensity of Thallos dyes over time for RGCs exposed to ambient or elevated pressure for 4 h plus treatment with vehicle or bisindolylmaleimide. ∗ p

    Article Snippet: Samples were then incubated overnight at 4°C in primary antibody solution (3% NHS and 0.1% Triton X-100 diluted in 1 × PBS) containing rabbit anti-Total Na/K-ATPase (2 μg/ml, Cat# ab58475, Abcam, Cambridge, United Kingdom), rabbit anti-α1 Na/K-ATPase (2.85 μg/ml, Cat# ANP-001, Alomone, Jerusalem, Israel); and mouse anti-β-Tubulin III (2 μg/ml, Cat# 801201, BioLegend, London, United Kingdom).

    Techniques: Inhibition, Fluorescence

    Na/K-ATPase inhibition reproduces cation dyshomeostasis and repolarization deficits. (A) Representative heat maps depicting the fluorescent signal of Thallos dye in RGCs exposed to ambient and elevated pressure for 4 h in the presence of vehicle or 20 μm ouabain or vehicle. Scale bar = 100 μM. (B) Line graph displaying the mean fluorescence intensity of Thallos dyes over time for RGCs exposed to ambient or elevated pressure for 4 h in the presence of vehicle or 20 μm ouabain. ∗ p

    Journal: Frontiers in Neuroscience

    Article Title: Impairment of Membrane Repolarization Accompanies Axon Transport Deficits in Glaucoma

    doi: 10.3389/fnins.2019.01139

    Figure Lengend Snippet: Na/K-ATPase inhibition reproduces cation dyshomeostasis and repolarization deficits. (A) Representative heat maps depicting the fluorescent signal of Thallos dye in RGCs exposed to ambient and elevated pressure for 4 h in the presence of vehicle or 20 μm ouabain or vehicle. Scale bar = 100 μM. (B) Line graph displaying the mean fluorescence intensity of Thallos dyes over time for RGCs exposed to ambient or elevated pressure for 4 h in the presence of vehicle or 20 μm ouabain. ∗ p

    Article Snippet: Samples were then incubated overnight at 4°C in primary antibody solution (3% NHS and 0.1% Triton X-100 diluted in 1 × PBS) containing rabbit anti-Total Na/K-ATPase (2 μg/ml, Cat# ab58475, Abcam, Cambridge, United Kingdom), rabbit anti-α1 Na/K-ATPase (2.85 μg/ml, Cat# ANP-001, Alomone, Jerusalem, Israel); and mouse anti-β-Tubulin III (2 μg/ml, Cat# 801201, BioLegend, London, United Kingdom).

    Techniques: Inhibition, Fluorescence