anti cleaved caspase 3  (Abcam)

 
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    Name:
    Anti active Caspase 3 antibody
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    AB2302
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    Structured Review

    Abcam anti cleaved caspase 3
    Effect of miR-1 silencing on glucose-induced apoptosis. (A) Effect of miR-1 silencing on glucose-induced mitochondrial dysfunction in H9C2 cells. (B) Quantification of fluorescence intensity. (C) The expression of apoptosis-associated proteins, including Bcl-2, Bax, total/cleaved <t>caspase-3,</t> total/cleaved caspase-9 and total/cleaved PARP in H9C2 cells. (D) Quantitative analysis of western blot analysis results. **P

    https://www.bioz.com/result/anti cleaved caspase 3/product/Abcam
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti cleaved caspase 3 - by Bioz Stars, 2021-06
    99/100 stars

    Images

    1) Product Images from "Downregulation of microRNA-1 attenuates glucose-induced apoptosis by regulating the liver X receptor α in cardiomyocytes"

    Article Title: Downregulation of microRNA-1 attenuates glucose-induced apoptosis by regulating the liver X receptor α in cardiomyocytes

    Journal: Experimental and Therapeutic Medicine

    doi: 10.3892/etm.2018.6388

    Effect of miR-1 silencing on glucose-induced apoptosis. (A) Effect of miR-1 silencing on glucose-induced mitochondrial dysfunction in H9C2 cells. (B) Quantification of fluorescence intensity. (C) The expression of apoptosis-associated proteins, including Bcl-2, Bax, total/cleaved caspase-3, total/cleaved caspase-9 and total/cleaved PARP in H9C2 cells. (D) Quantitative analysis of western blot analysis results. **P
    Figure Legend Snippet: Effect of miR-1 silencing on glucose-induced apoptosis. (A) Effect of miR-1 silencing on glucose-induced mitochondrial dysfunction in H9C2 cells. (B) Quantification of fluorescence intensity. (C) The expression of apoptosis-associated proteins, including Bcl-2, Bax, total/cleaved caspase-3, total/cleaved caspase-9 and total/cleaved PARP in H9C2 cells. (D) Quantitative analysis of western blot analysis results. **P

    Techniques Used: Fluorescence, Expressing, Western Blot

    2) Product Images from "Nanoparticle-mediated Gene Silencing Confers Radioprotection to Salivary Glands In Vivo"

    Article Title: Nanoparticle-mediated Gene Silencing Confers Radioprotection to Salivary Glands In Vivo

    Journal: Molecular Therapy

    doi: 10.1038/mt.2013.42

    Analysis of mouse SMG for off-target effects following siRNA–nanoparticle retroductal injections . ( a – c ) Apoptosis was measured by staining for caspase-3–positive cells at 1 week post-injection. Sections were prepared from ( a ) SMG
    Figure Legend Snippet: Analysis of mouse SMG for off-target effects following siRNA–nanoparticle retroductal injections . ( a – c ) Apoptosis was measured by staining for caspase-3–positive cells at 1 week post-injection. Sections were prepared from ( a ) SMG

    Techniques Used: Staining, Injection

    3) Product Images from "Cardiac spheroids as promising in vitro models to study the human heart microenvironment"

    Article Title: Cardiac spheroids as promising in vitro models to study the human heart microenvironment

    Journal: Scientific Reports

    doi: 10.1038/s41598-017-06385-8

    Cross-talk between different cell types in DOX-induced cardiotoxicity. ( a ) DOX-induced effects on cardiomyocytes occurs via ROS/caspase 3-mediated apoptosis. ( b ) EC-released NO protects cardiomyocytes against DOX-induced toxicity. ( c ) DOX-induced eNOS up-regulation in endothelial cells produces toxic NO species. ( d ) DOX-induced eNOS up-regulation in cardiac fibroblasts produces the majority of the toxic NO species. ( e ) DOX-induced cardiotoxic effects via ROS production in cardiac fibroblasts. At the molecular level, DOX/superoxide-induced apoptosis in cardiomyocytes can be either rescued by endothelial cell-released NO, via inhibition of a ROS-mediated toxic effect, or can be exacerbated by increased eNOS expression and peroxynitrous acid formation. Cardiac fibroblasts can trigger DOX/NO-toxic effects by overexpressing eNOS and increasing peroxynitrous acid generation, or could increase superoxide levels and therefore feedback to endothelial cell-mediated peroxynitrous acid and cardiomyocyte apoptosis.
    Figure Legend Snippet: Cross-talk between different cell types in DOX-induced cardiotoxicity. ( a ) DOX-induced effects on cardiomyocytes occurs via ROS/caspase 3-mediated apoptosis. ( b ) EC-released NO protects cardiomyocytes against DOX-induced toxicity. ( c ) DOX-induced eNOS up-regulation in endothelial cells produces toxic NO species. ( d ) DOX-induced eNOS up-regulation in cardiac fibroblasts produces the majority of the toxic NO species. ( e ) DOX-induced cardiotoxic effects via ROS production in cardiac fibroblasts. At the molecular level, DOX/superoxide-induced apoptosis in cardiomyocytes can be either rescued by endothelial cell-released NO, via inhibition of a ROS-mediated toxic effect, or can be exacerbated by increased eNOS expression and peroxynitrous acid formation. Cardiac fibroblasts can trigger DOX/NO-toxic effects by overexpressing eNOS and increasing peroxynitrous acid generation, or could increase superoxide levels and therefore feedback to endothelial cell-mediated peroxynitrous acid and cardiomyocyte apoptosis.

    Techniques Used: Inhibition, Expressing

    Doxorubicin-mediated apoptosis is inhibited by L-NIO. ( a ) Confocal analysis of hCSs and iCSs cultured with and without 20 μM DOX and 100 μM L-NIO and stained with antibodies against active caspase 3 (shown in red). ( b ) Statistical analysis ( n  = 3) of black-versus-white ratios of confocal images of hCSs and iCSs cultured and stained as described in panel (a). Data are presented as mean ± SEM. Scale bars: 200 μm. One-way ANOVA followed by Bonferroni’s multiple comparisons test. F (df 5, 12) = 1.153.
    Figure Legend Snippet: Doxorubicin-mediated apoptosis is inhibited by L-NIO. ( a ) Confocal analysis of hCSs and iCSs cultured with and without 20 μM DOX and 100 μM L-NIO and stained with antibodies against active caspase 3 (shown in red). ( b ) Statistical analysis ( n  = 3) of black-versus-white ratios of confocal images of hCSs and iCSs cultured and stained as described in panel (a). Data are presented as mean ± SEM. Scale bars: 200 μm. One-way ANOVA followed by Bonferroni’s multiple comparisons test. F (df 5, 12) = 1.153.

    Techniques Used: Cell Culture, Staining

    4) Product Images from "Electro-acupuncture promotes the proliferation of neural stem cells and the survival of neurons by downregulating miR-449a in rat with spinal cord injury"

    Article Title: Electro-acupuncture promotes the proliferation of neural stem cells and the survival of neurons by downregulating miR-449a in rat with spinal cord injury

    Journal: EXCLI Journal

    doi: 10.17179/excli2017-123

    Electro-acupuncture inhibited cell apoptosis and inflammation in ventral horn of injured spinal cord. A. Protein level of cleaved caspase 3, Bax and Bcl2 measured with Western blotting; B, C. Relative level of cleaved caspase 3 and ratio of Bax to Bcl2 quantified by densitometric analysis; D. Relative level of inflammatory factors TNF-α and IL-1β measured with Western blotting; E. Densitometric analysis results of the protein levels of TNF-α and IL-1β. *P
    Figure Legend Snippet: Electro-acupuncture inhibited cell apoptosis and inflammation in ventral horn of injured spinal cord. A. Protein level of cleaved caspase 3, Bax and Bcl2 measured with Western blotting; B, C. Relative level of cleaved caspase 3 and ratio of Bax to Bcl2 quantified by densitometric analysis; D. Relative level of inflammatory factors TNF-α and IL-1β measured with Western blotting; E. Densitometric analysis results of the protein levels of TNF-α and IL-1β. *P

    Techniques Used: Western Blot

    MiR-449 mediated the promotive effect of EA on the expression of NSC biomarker Nestin and neuron biomarker NeuN. A. miR-449a agomir treatment (20 μl 500 pmol, intraperitoneal injection, for 3 days after SCI) promoted miR-449a level; B-D. miR-449a agomir treatment significantly restored the elevated mRNA (B) and protein (C, D) levels of Nestin, NeuN and CGRP by EA; E. Representative immunohistochemical stain of Nestin, NeuN and CGRP in ventral horn of spinal cord; F-H. Quantification of immunohistochemical stain of Nestin (F), NeuN (G) and CGRP (H); I-L. Protein level of TNF-α, IL-1β, cleaved caspase 3, Bax and Bcl2 measured with Western blotting, and quantified based on densitometry analysis. *P
    Figure Legend Snippet: MiR-449 mediated the promotive effect of EA on the expression of NSC biomarker Nestin and neuron biomarker NeuN. A. miR-449a agomir treatment (20 μl 500 pmol, intraperitoneal injection, for 3 days after SCI) promoted miR-449a level; B-D. miR-449a agomir treatment significantly restored the elevated mRNA (B) and protein (C, D) levels of Nestin, NeuN and CGRP by EA; E. Representative immunohistochemical stain of Nestin, NeuN and CGRP in ventral horn of spinal cord; F-H. Quantification of immunohistochemical stain of Nestin (F), NeuN (G) and CGRP (H); I-L. Protein level of TNF-α, IL-1β, cleaved caspase 3, Bax and Bcl2 measured with Western blotting, and quantified based on densitometry analysis. *P

    Techniques Used: Expressing, Biomarker Assay, Injection, Immunohistochemistry, Staining, Western Blot

    5) Product Images from "Berberine Prolongs Mouse Heart Allograft Survival by Activating T Cell Apoptosis via the Mitochondrial Pathway"

    Article Title: Berberine Prolongs Mouse Heart Allograft Survival by Activating T Cell Apoptosis via the Mitochondrial Pathway

    Journal: Frontiers in Immunology

    doi: 10.3389/fimmu.2021.616074

    Berberine induces T cell apoptosis via the mitochondrial apoptosis pathway. (A) (i) KEGG functional categories of differentially expressed genes following berberine and saline treatment. The Y-axis represents the KEGG functional categories. (ii) KEGG analysis of the significantly altered signaling pathways in cell growth- and death-associated genes. The X-axis represents the rich ratio of the number of differentially expressed genes and the Y-axis represents the KEGG pathways. (iii) qPCR analysis of Bcl-2 and TNF-α mRNA expression in SPCs collected from heart transplant recipients treated with berberine or not. (B) T cell apoptosis assays in vivo . (i) SPCs and (ii) LNCs were collected at POD 7. The percentages of apoptotic CD4 + and CD8 + T cells were determined by flow cytometry (n = 3 mice/group). (C) T cell apoptosis assay in vitro . T cells from naïve C57BL/6 mice were co-stimulated with anti-CD3/CD28 Abs in the absence or presence of berberine. The percentages of apoptotic (i) CD4 + and (ii) CD8 + T cells were determined by flow cytometry. (D) Berberine activates the mitochondrial apoptosis pathway in vivo . (i) Relative protein expression of Bcl-2, Bax, cytochrome c, cleaved-caspase-3, and cleaved-PARP in SPC. (ii) β-actin was used as a loading control (n = 3 mice/group), and OD values (relative to β-actin) are presented as means ± SEMs. * p
    Figure Legend Snippet: Berberine induces T cell apoptosis via the mitochondrial apoptosis pathway. (A) (i) KEGG functional categories of differentially expressed genes following berberine and saline treatment. The Y-axis represents the KEGG functional categories. (ii) KEGG analysis of the significantly altered signaling pathways in cell growth- and death-associated genes. The X-axis represents the rich ratio of the number of differentially expressed genes and the Y-axis represents the KEGG pathways. (iii) qPCR analysis of Bcl-2 and TNF-α mRNA expression in SPCs collected from heart transplant recipients treated with berberine or not. (B) T cell apoptosis assays in vivo . (i) SPCs and (ii) LNCs were collected at POD 7. The percentages of apoptotic CD4 + and CD8 + T cells were determined by flow cytometry (n = 3 mice/group). (C) T cell apoptosis assay in vitro . T cells from naïve C57BL/6 mice were co-stimulated with anti-CD3/CD28 Abs in the absence or presence of berberine. The percentages of apoptotic (i) CD4 + and (ii) CD8 + T cells were determined by flow cytometry. (D) Berberine activates the mitochondrial apoptosis pathway in vivo . (i) Relative protein expression of Bcl-2, Bax, cytochrome c, cleaved-caspase-3, and cleaved-PARP in SPC. (ii) β-actin was used as a loading control (n = 3 mice/group), and OD values (relative to β-actin) are presented as means ± SEMs. * p

    Techniques Used: Functional Assay, Real-time Polymerase Chain Reaction, Expressing, In Vivo, Flow Cytometry, Mouse Assay, Apoptosis Assay, In Vitro

    Phenotypic and functional characteristics of allograft-infiltrating CD4 + or CD8 + T cells. Allografts were recovered at POD 7, and POD 100 syngeneic grafts are shown for comparison. (A) (i) Immunofluorescent staining of CD4 (red), KI67 (green), and 4′,6-diamidino-2-phenylindole (DAPI, blue) in grafts. (ii) Immunofluorescent staining of CD8 (red), KI67 (green), and DAPI in grafts (Scale bar = 200 μm; original magnification: ×200). (B) Proportion and absolute number of graft-infiltrating (i) CD4 + T cells and their expression of (ii) KI67, and proportion and absolute number of graft-infiltrating (iii) CD8 + T cells and their expression of (iv) KI67 (n = 3 mice/group). (C) Proportion of (i) IFN-γ and (ii) cleaved-caspase-3 in graft-infiltrating CD3 + T cells (n = 3 mice/group). (D) (i) Cleaved-caspase-3 and cleaved-PARP protein expression in grafts. Myocardial cell apoptosis co-immunofluorescence staining and expression of (ii) cleaved-caspase-3 and (iii) cleaved-PARP (n = 3 mice/group). (E) Relative mRNA expression of IFN-γ , IL-6, IL-10 , Foxp3 , and FasL in grafts measured by qPCR (n = 3 mice/group). SPCs, spleen cells; LNCs, lymph node cells; POD, post-operative day. * p
    Figure Legend Snippet: Phenotypic and functional characteristics of allograft-infiltrating CD4 + or CD8 + T cells. Allografts were recovered at POD 7, and POD 100 syngeneic grafts are shown for comparison. (A) (i) Immunofluorescent staining of CD4 (red), KI67 (green), and 4′,6-diamidino-2-phenylindole (DAPI, blue) in grafts. (ii) Immunofluorescent staining of CD8 (red), KI67 (green), and DAPI in grafts (Scale bar = 200 μm; original magnification: ×200). (B) Proportion and absolute number of graft-infiltrating (i) CD4 + T cells and their expression of (ii) KI67, and proportion and absolute number of graft-infiltrating (iii) CD8 + T cells and their expression of (iv) KI67 (n = 3 mice/group). (C) Proportion of (i) IFN-γ and (ii) cleaved-caspase-3 in graft-infiltrating CD3 + T cells (n = 3 mice/group). (D) (i) Cleaved-caspase-3 and cleaved-PARP protein expression in grafts. Myocardial cell apoptosis co-immunofluorescence staining and expression of (ii) cleaved-caspase-3 and (iii) cleaved-PARP (n = 3 mice/group). (E) Relative mRNA expression of IFN-γ , IL-6, IL-10 , Foxp3 , and FasL in grafts measured by qPCR (n = 3 mice/group). SPCs, spleen cells; LNCs, lymph node cells; POD, post-operative day. * p

    Techniques Used: Functional Assay, Staining, Expressing, Mouse Assay, Immunofluorescence, Real-time Polymerase Chain Reaction

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    Article Snippet: Using the method of sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), the protein samples (30 μg) were separated and subsequent transfected to polyvinylidene fluoride (PVDF) membranes. .. The membranes were blocked with 5% BSA, and incubated with the diluted primary antibodies of CyclinD1 (ab137875), p53 (ab131442), p21 (ab109199), pro-Caspase-3 (ab32150), cleaved-Caspase-3 (ab2302), pro-Caspase-9 (ab138412), cleaved-Caspase-9 (ab2324), matrix metalloproteinase-2 (MMP-2, ab37150), MMP-9 (ab38898), Vimentin (ab16700), phosphorylated (p)-p56 (ab28856), t-p56 (ab32536), p-IκBα (ab133462), t-IκBα (ab32518), β-catenin (ab32572) and β-actin (ab8227, Abcam) overnight at 4 °C. .. After incubation with primary antibodies, the membranes were washed several times with TBST, and the diluted second antibody of horseradish peroxidase (HRP)-conjugated goat anti-rabbit IgG (ab205718, 1:2000, Abcam) was added to incubate for another 1 h at room temperature.

    Article Title: Downregulation of microRNA-1 attenuates glucose-induced apoptosis by regulating the liver X receptor α in cardiomyocytes
    Article Snippet: Proteins (40 µg/lane) were separated by 10–15% SDS-PAGE and transferred to polyvinylidene difluoride membranes (EMD Millipore, Billerica, MA, USA). .. Membranes were incubated with primary antibodies overnight at 4°C and subsequently incubated with horseradish peroxidase-conjugated goat anti-rabbit/mouse immunoglobulin G secondary antibodies (1:5,000; Beyotime Institute of Biotechnology) at room temperature for 45 min. Anti-cleaved-poly (adenosine diphosphate-ribose) polymerase (PARP; cat. no. ab32561; 1:1,000), anti-cleaved-caspase-3 (cat. no. ab2302; 1:1,000), anti-cleaved-caspase-9 (cat. no. ab25758; 1:1,000) and anti-LXRα (cat. no. ab3585; 1:300) primary antibodies were obtained from Abcam (Cambridge, MA, USA). .. Anti-PARP (cat. no. 9532; 1:5,000), anti-caspase-3 (cat. no. 9662; 1:5,000) and anti-caspase-9 (cat. no. 9508; 1:5,000) primary antibodies were obtained from Cell Signaling Technology, Inc. (Danvers, MA, USA).

    Article Title: Mustard Leaf Extract Suppresses Psychological Stress in Chronic Restraint Stress-Subjected Mice by Regulation of Stress Hormone, Neurotransmitters, and Apoptosis
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    Article Title: Novel ASK1 inhibitor AGI‐1067 improves AGE‐induced cardiac dysfunction by inhibiting MKKs/p38 MAPK and NF‐κB apoptotic signaling
    Article Snippet: .. The membranes were incubated with primary antibodies against MKK3 (Sigma‐Aldrich; 1 : 500), phospho‐MKK3 (Sigma‐Aldrich; 1 : 500), MKK6 (Sigma‐Aldrich; 1 : 500), phospho‐MKK6 (Sigma‐Aldrich; 1 : 500), p38 (Cell Signaling Technology; 1 : 250), phospho‐p38 (Cell Signaling Technology, Danvers, MA, USA; 1 : 250), active caspase‐3 (Abcam; 1 : 1000), p65 (Abcam; 1 : 1000), histone H3 (Abcam; 1 : 1000) and glyceraldehyde 3‐phosphate dehydrogenase (GAPDH; Abcam; 1 : 500) at 4 °C for 10 h. Then the membranes were washed by Tris‐buffered saline–Tween‐20 (0.02%, TBST). .. Horseradish peroxidase‐conjugated secondary antibodies were used to incubate the membranes at room temperature for 2 h. SuperSignal West Pico chemiluminescence reagent (Thermo Fisher Scientific, Carlsbad, CA, USA) was used to develop the membranes, and the immunoblots were visualized on X‐ray films.

    SDS Page:

    Article Title: Mustard Leaf Extract Suppresses Psychological Stress in Chronic Restraint Stress-Subjected Mice by Regulation of Stress Hormone, Neurotransmitters, and Apoptosis
    Article Snippet: Protein concentration was determined using a bicinchoninic acid assay (BCA) (GenDEPOT, Barker, TX, USA). .. A quantity of 5–20 μg total protein was loaded onto a 4–20% gel for SDS-PAGE, transferred to a PVDF membrane, blocked with skimmed milk for 1 h, and then incubated overnight with primary antibodies against the following proteins at 4 °C: BDNF, Bcl-2, Bax, Caspase-3, PARP, and β-actin (Abcam, Cambridge, MA, USA). .. The membranes were then incubated with secondary antibodies for 1 h and developed using an enhanced chemiluminescence kit (Pierce, Rockford, IL, USA) and a ChemiDoc image detector (Bio-Rad, Hercules, CA, USA).

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    Irradiation:

    Article Title: Nanoparticle-mediated Gene Silencing Confers Radioprotection to Salivary Glands In Vivo
    Article Snippet: Endogenous caspase-3 activation was examined following Nkcc1 siRNA–nanoparticle injections using the SignalStain Cleaved Caspase-3 detection kit (Cell Signaling Technology, Danvers, MA) on paraffin-embedded tissue sections. .. For detection of the active form of caspase-3 in cells undergoing apoptosis following irradiation, a rabbit Caspase-3 antibody (ab2302; Abcam) was used. .. After deparaffinization and heat-induced antigen retrieval steps, the slides were immersed in 3% H2 O2 to quench endogenous peroxidase, and treated with the Avidin/Biotin blocking kit (SP-2001; Vector Laboratories).

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  • 93
    Abcam anti active caspase 3 antibody
    Knockdown VEGFA inhibited autophagy and increased apoptosis. ( A ) Western blotting analysis of autophagy biomarkers LC3I-II, Beclin-1 and p62 in SKOV3 and SKOV3/DDP cells. ( B ) Western blotting analysis of Beclin-1 and LC3I-II in SKVOK3 cells treated with DDP (50µM), PTX (40µM), and ADM (20µM) for 48 hours. ( C ) Western blotting analysis of autophagy biomarkers LC3I-II and p62 in SKOV3/DDP cells transfected with shVEGFA and treated with 3-MA. ( D ) Western blotting analysis of apoptotic biomarkers <t>caspase-3</t> and cleaved caspase-3 in SKOV3/DDP cells transfected with shVEGFA and treated with 3-MA. ( E ) Flow cytometry analysis of apoptosis of SKOV3/DDP cells after transfected with shVEGFA or control treated with 3-MA. ( F ) Statistical analysis of apoptosis ratio of SKOV3/DDP cells transfected with shVEGFA and treated with 3-MA. Results are representative of three independent experiments. ** P
    Anti Active Caspase 3 Antibody, supplied by Abcam, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti active caspase 3 antibody/product/Abcam
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti active caspase 3 antibody - by Bioz Stars, 2021-06
    93/100 stars
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    98
    Abcam primary antibodies against active caspase 3
    CBF-induced HT29 apoptosis is neither <t>caspase-3</t> nor AIF dependent. (a) The inhibition of caspase-3/7 activity in CBF-treated HT29 cells. Treated and untreated cells were lysed and the assessment of caspase-3 luciferase intensity showed that caspase-3/7 activity was significantly downregulated after 24-hour treatment ( N = 9, * P
    Primary Antibodies Against Active Caspase 3, supplied by Abcam, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/primary antibodies against active caspase 3/product/Abcam
    Average 98 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    primary antibodies against active caspase 3 - by Bioz Stars, 2021-06
    98/100 stars
      Buy from Supplier

    Image Search Results


    Knockdown VEGFA inhibited autophagy and increased apoptosis. ( A ) Western blotting analysis of autophagy biomarkers LC3I-II, Beclin-1 and p62 in SKOV3 and SKOV3/DDP cells. ( B ) Western blotting analysis of Beclin-1 and LC3I-II in SKVOK3 cells treated with DDP (50µM), PTX (40µM), and ADM (20µM) for 48 hours. ( C ) Western blotting analysis of autophagy biomarkers LC3I-II and p62 in SKOV3/DDP cells transfected with shVEGFA and treated with 3-MA. ( D ) Western blotting analysis of apoptotic biomarkers caspase-3 and cleaved caspase-3 in SKOV3/DDP cells transfected with shVEGFA and treated with 3-MA. ( E ) Flow cytometry analysis of apoptosis of SKOV3/DDP cells after transfected with shVEGFA or control treated with 3-MA. ( F ) Statistical analysis of apoptosis ratio of SKOV3/DDP cells transfected with shVEGFA and treated with 3-MA. Results are representative of three independent experiments. ** P

    Journal: OncoTargets and therapy

    Article Title: Inhibition of VEGFA Increases the Sensitivity of Ovarian Cancer Cells to Chemotherapy by Suppressing VEGFA-Mediated Autophagy

    doi: 10.2147/OTT.S250392

    Figure Lengend Snippet: Knockdown VEGFA inhibited autophagy and increased apoptosis. ( A ) Western blotting analysis of autophagy biomarkers LC3I-II, Beclin-1 and p62 in SKOV3 and SKOV3/DDP cells. ( B ) Western blotting analysis of Beclin-1 and LC3I-II in SKVOK3 cells treated with DDP (50µM), PTX (40µM), and ADM (20µM) for 48 hours. ( C ) Western blotting analysis of autophagy biomarkers LC3I-II and p62 in SKOV3/DDP cells transfected with shVEGFA and treated with 3-MA. ( D ) Western blotting analysis of apoptotic biomarkers caspase-3 and cleaved caspase-3 in SKOV3/DDP cells transfected with shVEGFA and treated with 3-MA. ( E ) Flow cytometry analysis of apoptosis of SKOV3/DDP cells after transfected with shVEGFA or control treated with 3-MA. ( F ) Statistical analysis of apoptosis ratio of SKOV3/DDP cells transfected with shVEGFA and treated with 3-MA. Results are representative of three independent experiments. ** P

    Article Snippet: AntibodiesAnti-VEGFA (ab1316), anti-caspase-3 (ab179517) antibodies, and anti-cleaved caspase-3 (ab2302) were purchased from Abcam (USA).

    Techniques: Western Blot, Transfection, Flow Cytometry

    CBF-induced HT29 apoptosis is neither caspase-3 nor AIF dependent. (a) The inhibition of caspase-3/7 activity in CBF-treated HT29 cells. Treated and untreated cells were lysed and the assessment of caspase-3 luciferase intensity showed that caspase-3/7 activity was significantly downregulated after 24-hour treatment ( N = 9, * P

    Journal: Evidence-based Complementary and Alternative Medicine : eCAM

    Article Title: The Mechanisms of Chansu in Inducing Efficient Apoptosis in Colon Cancer Cells

    doi: 10.1155/2013/849054

    Figure Lengend Snippet: CBF-induced HT29 apoptosis is neither caspase-3 nor AIF dependent. (a) The inhibition of caspase-3/7 activity in CBF-treated HT29 cells. Treated and untreated cells were lysed and the assessment of caspase-3 luciferase intensity showed that caspase-3/7 activity was significantly downregulated after 24-hour treatment ( N = 9, * P

    Article Snippet: Cells or tissues were sequentially incubated with corresponding primary antibodies against active caspase-3 (1 : 100; Abcam, Sapphire Bioscience, NSW, Australia), AIF (1 : 100; Cell Signalling, MA, USA), or HIF-1α (1 : 100; Novus, CO, USA), followed by incubation with a secondary antibody FITC or Texas Red-conjugated goat anti-rabbit IgG (1 : 2000; Abcam).

    Techniques: Inhibition, Activity Assay, Luciferase

    CBF-induced apoptosis is both caspase-3 dependent and independent in HCT116 cells. (a) Expression of active caspase-3 detected by confocal microscopy. HCT116 cells were treated with 0 or 1 µ M of CBF for 24 hours and analysed by immunofluorescence. The expression of active caspase-3 (green fluorescence) was only observed in treated HCT116. Cell nuclei were stained with DAPI (blue fluorescence). Scale bars equal 10 μ m. (b) Detection of caspase-3/7 activity via a time course. A significant increase of caspase-3/7 activity was found after 24-hour-CBF exposure ( N = 9, * P

    Journal: Evidence-based Complementary and Alternative Medicine : eCAM

    Article Title: The Mechanisms of Chansu in Inducing Efficient Apoptosis in Colon Cancer Cells

    doi: 10.1155/2013/849054

    Figure Lengend Snippet: CBF-induced apoptosis is both caspase-3 dependent and independent in HCT116 cells. (a) Expression of active caspase-3 detected by confocal microscopy. HCT116 cells were treated with 0 or 1 µ M of CBF for 24 hours and analysed by immunofluorescence. The expression of active caspase-3 (green fluorescence) was only observed in treated HCT116. Cell nuclei were stained with DAPI (blue fluorescence). Scale bars equal 10 μ m. (b) Detection of caspase-3/7 activity via a time course. A significant increase of caspase-3/7 activity was found after 24-hour-CBF exposure ( N = 9, * P

    Article Snippet: Cells or tissues were sequentially incubated with corresponding primary antibodies against active caspase-3 (1 : 100; Abcam, Sapphire Bioscience, NSW, Australia), AIF (1 : 100; Cell Signalling, MA, USA), or HIF-1α (1 : 100; Novus, CO, USA), followed by incubation with a secondary antibody FITC or Texas Red-conjugated goat anti-rabbit IgG (1 : 2000; Abcam).

    Techniques: Expressing, Confocal Microscopy, Immunofluorescence, Fluorescence, Staining, Activity Assay