Journal: Hepatology Communications

Article Title: Overexpression of TNFα in TNF ∆ARE+/− mice increases hepatic periportal inflammation and alters bile acid signaling in mice

doi: 10.1097/HC9.0000000000000589

Figure Lengend Snippet: Chronic production of TNFα dysregulates hepatic FXR and bile acid synthesis. (A) Relative mRNA expression of hepatic FXR and bile acid synthesis pathways. (B) Western analysis and quantification of BSEP, FXR, NTCP, and SHP protein expression with representative GAPDH loading control. All proteins were normalized to GAPDH expression. (C) LC-MS of hepatic bile acid concentrations. Data are presented as mean ± SEM and were statistically analyzed using the Student t test, * p < 0.05, ** p < 0.01, *** p < 0.001, N = 3–9/group. Abbreviations: FXR, farnesoid X receptor; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; LC-MS, liquid chromatography mass spectrometry.

Article Snippet: Western blots were performed using separate gels for each protein analyzed and liver homogenate using 30 μg of protein per lane as described., The antibodies used in this study are glyceraldehyde 3-phosphate dehydrogenase (GAPDH (1:20,000, MAB374, Millipore)), SHP (1:1000, catalog number PA5-76632, Invitrogen/Thermofisher), BSEP (1:1000, catalog number NBP1-89319, Novus Biologicals), FXR (1:1000, catalog number sc-25309, Santa Cruz Biotechnologies), and NTCP (1:1000, catalog number PA5-80001, Invitrogen/Thermofisher).

Techniques: Expressing, Western Blot, Control, Liquid Chromatography with Mass Spectroscopy, Liquid Chromatography, Mass Spectrometry

Journal: Scientific Reports

Article Title: Protective effects of cilostazol via the HNF1α/FXR signalling pathway and anti-apoptotic mechanisms in a rat model of estrogen-induced intrahepatic cholestasis

doi: 10.1038/s41598-024-72729-w

Figure Lengend Snippet: Effect of CTZ on the hepatic protein expression of ( A ) HNF1α, ( B ) FXR, ( C ) BSEP, ( D ) CYP3A1, and ( E ) western blot images. EE ethinyl estradiol, UDCA ursodeoxycholic acid, CTZ cilostazol, FXR farnesoid X receptor, HNF1α hepatocyte nuclear factor 1α, CYP cytochrome P450, BSEP bile salt export pump. a, b, c, d Significantly different from normal, EE-induced EIC, EE + UDCA, and EE + CTZ (5 mg/kg) groups at p < 0.05, respectively.

Article Snippet: The membranes were incubated with primary antibodies against HNF1α (Abcam, USA; CAT no: ab128912), FXR (Santa Cruz, USA; CAT no: sc-25309), CYP3A1 (Santa Cruz, USA; CAT no: sc-53246), BSEP (Proteinintech, USA; CAT no: 67512-1-lg), and β-actin (dilution 1:1000) overnight at 4 °C.

Techniques: Expressing, Western Blot