Anti Kv4 3 Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "Calcium/calmodulin-dependent protein kinase II associates with the K+ channel isoform Kv4.3 in adult rat optic nerve"
Article Title: Calcium/calmodulin-dependent protein kinase II associates with the K+ channel isoform Kv4.3 in adult rat optic nerve
Journal: Frontiers in Neuroanatomy
Figure Legend Snippet: Kv4.3 immunopositivity in optic nerve axons. Confocally imaged portion of optic nerve that was longitudinally sectioned and immunostained with antibodies directed against Kv4.3 (upper panel) and NF-70 (middle panel). The upper and middle panels are superimposed in the lower panel (“merge”). The tip of each arrowhead points to an axon (based on NF-70 immunopositivity). Arrowheads of a given color point at the same axon. Different colors are used to point at different axons. Otherwise, the color and direction of the arrowheads (upward or downward) are arbitrary. The calibration bar in the lower panel shows 10 μm and applies to all panels.
Figure Legend Snippet: Control for non-specific binding by rabbit anti-Kv4.3 antibody. (A) Fields of optic nerve that were longitudinally sectioned and processed as in Figure 3 , except that the anti-Kv4.3 antibody in the upper panel was preincubated with immunogen, and the anti-Kv4.3 antibody in the lower panel was not (as in Figure 3 ). These fields were imaged at identical confocal microscope settings (laser intensity, photomultiplier gain, and pinhole diameter). The calibration bar in the lower panel shows 10 μm and applies to both panels. (B) Western-blotted lanes of optic nerve lysate. The lanes were processed identically except that the anti-Kv4.3 antibody was either preincubated with immunogen (in the lane marked “+immunogen”) or it was used without this preincubation (in the lane marked “–immunogen”). To gauge protein load, both lanes were probed with rat anti-myelin basic protein antibody. The fluorescence of the anti-rabbit secondary antibody is pseudo-colored red. The fluorescence of the anti-rat secondary antibody is pseudo-colored blue. The molecular weight and migration distance of protein standards that were run in an adjacent lane are shown along the right edge.
Techniques Used: Binding Assay, Microscopy, Western Blot, Fluorescence, Molecular Weight, Migration
Figure Legend Snippet: Reciprocal co-immunoprecipitation of Kv4.3 and CaMKII. Western-blotted lanes (1-7), with one lane cut vertically into two parts (lanes 3a, 3b). The material that was loaded into the gel lanes for electrophoresis, and the antibody used to probe each Western-blotted lane, are listed above and below the lanes, respectively. The lanes were loaded with either optic nerve lysate (lanes 3a, 3b, 6), elute from the anti-Kv4.3 antibody pull-down (lanes 1, 5), or elute from the anti-CaMKII antibody pull-down (lanes 2, 4, 7). The Western-blotted lanes were probed with rabbit anti-Kv4.3 antibody (“Kv4.3”) and anti-rabbit secondary antibody, mouse anti-CaMKII antibody (“CaMKII”) and anti-mouse secondary antibody, or anti-rabbit secondary antibody without primary antibody (“2° only”). The molecular weight and migration distance of protein standards that were run in an adjacent lane are shown along the left edge. Note that the faint bands around 60 kDa in lane 3a are absent in lane 3b.
Techniques Used: Immunoprecipitation, Western Blot, Electrophoresis, Molecular Weight, Migration