anti kv4 3 antibody  (Alomone Labs)


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    Structured Review

    Alomone Labs anti kv4 3 antibody
    <t>Kv4.3</t> immunopositivity in optic nerve axons. Confocally imaged portion of optic nerve that was longitudinally sectioned and immunostained with <t>antibodies</t> directed against Kv4.3 (upper panel) and NF-70 (middle panel). The upper and middle panels are superimposed in the lower panel (“merge”). The tip of each arrowhead points to an axon (based on NF-70 immunopositivity). Arrowheads of a given color point at the same axon. Different colors are used to point at different axons. Otherwise, the color and direction of the arrowheads (upward or downward) are arbitrary. The calibration bar in the lower panel shows 10 μm and applies to all panels.
    Anti Kv4 3 Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti kv4 3 antibody/product/Alomone Labs
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti kv4 3 antibody - by Bioz Stars, 2022-11
    95/100 stars

    Images

    1) Product Images from "Calcium/calmodulin-dependent protein kinase II associates with the K+ channel isoform Kv4.3 in adult rat optic nerve"

    Article Title: Calcium/calmodulin-dependent protein kinase II associates with the K+ channel isoform Kv4.3 in adult rat optic nerve

    Journal: Frontiers in Neuroanatomy

    doi: 10.3389/fnana.2022.958986

    Kv4.3 immunopositivity in optic nerve axons. Confocally imaged portion of optic nerve that was longitudinally sectioned and immunostained with antibodies directed against Kv4.3 (upper panel) and NF-70 (middle panel). The upper and middle panels are superimposed in the lower panel (“merge”). The tip of each arrowhead points to an axon (based on NF-70 immunopositivity). Arrowheads of a given color point at the same axon. Different colors are used to point at different axons. Otherwise, the color and direction of the arrowheads (upward or downward) are arbitrary. The calibration bar in the lower panel shows 10 μm and applies to all panels.
    Figure Legend Snippet: Kv4.3 immunopositivity in optic nerve axons. Confocally imaged portion of optic nerve that was longitudinally sectioned and immunostained with antibodies directed against Kv4.3 (upper panel) and NF-70 (middle panel). The upper and middle panels are superimposed in the lower panel (“merge”). The tip of each arrowhead points to an axon (based on NF-70 immunopositivity). Arrowheads of a given color point at the same axon. Different colors are used to point at different axons. Otherwise, the color and direction of the arrowheads (upward or downward) are arbitrary. The calibration bar in the lower panel shows 10 μm and applies to all panels.

    Techniques Used:

    Control for non-specific binding by rabbit anti-Kv4.3 antibody. (A) Fields of optic nerve that were longitudinally sectioned and processed as in Figure 3 , except that the anti-Kv4.3 antibody in the upper panel was preincubated with immunogen, and the anti-Kv4.3 antibody in the lower panel was not (as in Figure 3 ). These fields were imaged at identical confocal microscope settings (laser intensity, photomultiplier gain, and pinhole diameter). The calibration bar in the lower panel shows 10 μm and applies to both panels. (B) Western-blotted lanes of optic nerve lysate. The lanes were processed identically except that the anti-Kv4.3 antibody was either preincubated with immunogen (in the lane marked “+immunogen”) or it was used without this preincubation (in the lane marked “–immunogen”). To gauge protein load, both lanes were probed with rat anti-myelin basic protein antibody. The fluorescence of the anti-rabbit secondary antibody is pseudo-colored red. The fluorescence of the anti-rat secondary antibody is pseudo-colored blue. The molecular weight and migration distance of protein standards that were run in an adjacent lane are shown along the right edge.
    Figure Legend Snippet: Control for non-specific binding by rabbit anti-Kv4.3 antibody. (A) Fields of optic nerve that were longitudinally sectioned and processed as in Figure 3 , except that the anti-Kv4.3 antibody in the upper panel was preincubated with immunogen, and the anti-Kv4.3 antibody in the lower panel was not (as in Figure 3 ). These fields were imaged at identical confocal microscope settings (laser intensity, photomultiplier gain, and pinhole diameter). The calibration bar in the lower panel shows 10 μm and applies to both panels. (B) Western-blotted lanes of optic nerve lysate. The lanes were processed identically except that the anti-Kv4.3 antibody was either preincubated with immunogen (in the lane marked “+immunogen”) or it was used without this preincubation (in the lane marked “–immunogen”). To gauge protein load, both lanes were probed with rat anti-myelin basic protein antibody. The fluorescence of the anti-rabbit secondary antibody is pseudo-colored red. The fluorescence of the anti-rat secondary antibody is pseudo-colored blue. The molecular weight and migration distance of protein standards that were run in an adjacent lane are shown along the right edge.

    Techniques Used: Binding Assay, Microscopy, Western Blot, Fluorescence, Molecular Weight, Migration

    Reciprocal co-immunoprecipitation of Kv4.3 and CaMKII. Western-blotted lanes (1-7), with one lane cut vertically into two parts (lanes 3a, 3b). The material that was loaded into the gel lanes for electrophoresis, and the antibody used to probe each Western-blotted lane, are listed above and below the lanes, respectively. The lanes were loaded with either optic nerve lysate (lanes 3a, 3b, 6), elute from the anti-Kv4.3 antibody pull-down (lanes 1, 5), or elute from the anti-CaMKII antibody pull-down (lanes 2, 4, 7). The Western-blotted lanes were probed with rabbit anti-Kv4.3 antibody (“Kv4.3”) and anti-rabbit secondary antibody, mouse anti-CaMKII antibody (“CaMKII”) and anti-mouse secondary antibody, or anti-rabbit secondary antibody without primary antibody (“2° only”). The molecular weight and migration distance of protein standards that were run in an adjacent lane are shown along the left edge. Note that the faint bands around 60 kDa in lane 3a are absent in lane 3b.
    Figure Legend Snippet: Reciprocal co-immunoprecipitation of Kv4.3 and CaMKII. Western-blotted lanes (1-7), with one lane cut vertically into two parts (lanes 3a, 3b). The material that was loaded into the gel lanes for electrophoresis, and the antibody used to probe each Western-blotted lane, are listed above and below the lanes, respectively. The lanes were loaded with either optic nerve lysate (lanes 3a, 3b, 6), elute from the anti-Kv4.3 antibody pull-down (lanes 1, 5), or elute from the anti-CaMKII antibody pull-down (lanes 2, 4, 7). The Western-blotted lanes were probed with rabbit anti-Kv4.3 antibody (“Kv4.3”) and anti-rabbit secondary antibody, mouse anti-CaMKII antibody (“CaMKII”) and anti-mouse secondary antibody, or anti-rabbit secondary antibody without primary antibody (“2° only”). The molecular weight and migration distance of protein standards that were run in an adjacent lane are shown along the left edge. Note that the faint bands around 60 kDa in lane 3a are absent in lane 3b.

    Techniques Used: Immunoprecipitation, Western Blot, Electrophoresis, Molecular Weight, Migration

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    Alomone Labs anti kv4 3 antibody
    <t>Kv4.3</t> immunopositivity in optic nerve axons. Confocally imaged portion of optic nerve that was longitudinally sectioned and immunostained with <t>antibodies</t> directed against Kv4.3 (upper panel) and NF-70 (middle panel). The upper and middle panels are superimposed in the lower panel (“merge”). The tip of each arrowhead points to an axon (based on NF-70 immunopositivity). Arrowheads of a given color point at the same axon. Different colors are used to point at different axons. Otherwise, the color and direction of the arrowheads (upward or downward) are arbitrary. The calibration bar in the lower panel shows 10 μm and applies to all panels.
    Anti Kv4 3 Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti kv4 3 antibody/product/Alomone Labs
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti kv4 3 antibody - by Bioz Stars, 2022-11
    95/100 stars
      Buy from Supplier

    94
    Alomone Labs primary antibodies against kca 3 1
    Effects of a 24 h treatment with various signaling pathway inhibitors on IL-10 and IL-8 expression and effects of the 24 h treatment with the <t>ERK1/2</t> inhibitor, SCH772984 and JNK inhibitor, SP600125 on IL-10 and IL-8 secretion in THP-1-derived M 2 macrophages. ( A , D ): Real-time PCR examination of IL-10 ( A ) and IL-8 ( D ) expression in SCH772964 (1 μM)-, SP600125 (10 μM)-, PD169316 (10 μM)-, LY294002 (10 μM)-, AZD5363 (2 μM)-, everolimus (10 nM)-, LY364947 (10 μM)-, ciclosporin A (CsA, 1 μM)-, T-5224 (10 μM)-, and Bay11-7082 (10 μM)-treated THP-1-derived M 2 macrophages for 24 h. Relative mRNA expression in the vehicle control is expressed as 1.0 (n = 4 for each). ( B , C , E , F ): Quantitative detection of IL-10 ( B , C ) and/or IL-8 ( E , F ) secretion by an ELISA assay in SCH772964 ( B , E )- and SP600125 ( C , F )-treated THP-1-derived M 2 macrophages. Relative cytokine secretion in the vehicle control is expressed as 1.0 (n = 4 for each). **: p
    Primary Antibodies Against Kca 3 1, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/primary antibodies against kca 3 1/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
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    primary antibodies against kca 3 1 - by Bioz Stars, 2022-11
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    Alomone Labs anti kv1 3 kcna3 extracellular antibody
    Expression of <t>Kv1.3</t> in human pancreatic cancer tissue. Expression of Kv1.3 in human pancreatic tissue. Panel ( a ), high expression level of Kv1.3 in human pancreatic cancer tissue. ( n = 33 out of 55, scale bar = 50 µm). Panel ( b ), magnification of panel ( a ), scale bar = 20 µm. Panel ( c ), low expression level of Kv1.3 in human pancreatic cancer tissue. ( n = 22 out of 55, scale bar = 50 µm). Panel ( d ), magnification of panel c, scale bar = 20 µm. Panel ( e ), Immunoglobulin G (IgG) control with magnification (Panel ( f )). Panel ( g ) shows a pancreas adenocarcinoma ductule with high expression of Kv1.3 (brown color) and adjacent normal pancreas parenchyma without Kv1.3 expression (black arrow), scale bar = 50 µm. Panel ( h ), magnification of panel ( g ), scale bar = 20 µm.
    Anti Kv1 3 Kcna3 Extracellular Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti kv1 3 kcna3 extracellular antibody/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti kv1 3 kcna3 extracellular antibody - by Bioz Stars, 2022-11
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    Alomone Labs anti cav1 2 cacna1c antibody
    Analysis of mRNA levels and the expression of different VDCC subtypes in adrenal medullae of WT and 3xTg mice over 12 m age. ( A ) Normalized mRNA levels of different VDCCs in adrenal medullae from WT and 3xTg mice > 12 m. Both medullas of the same mouse were homogenized, so each dot represents an individual mouse. ( B ) Representative image of WT and 3xTg adrenal medulla stained with <t>anti-Cav1.3</t> (top) and <t>anti</t> Cav2.1 (bottom) + DAPI (nuclei). ( C ) IntDen quantification of calcium channel expression. Each dot represents an image analyzed, and the bars represent mean ± SEM. A Student’s t -test was used to statistically compare the WT and 3xTg groups. ** p
    Anti Cav1 2 Cacna1c Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti cav1 2 cacna1c antibody/product/Alomone Labs
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti cav1 2 cacna1c antibody - by Bioz Stars, 2022-11
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    Image Search Results


    Kv4.3 immunopositivity in optic nerve axons. Confocally imaged portion of optic nerve that was longitudinally sectioned and immunostained with antibodies directed against Kv4.3 (upper panel) and NF-70 (middle panel). The upper and middle panels are superimposed in the lower panel (“merge”). The tip of each arrowhead points to an axon (based on NF-70 immunopositivity). Arrowheads of a given color point at the same axon. Different colors are used to point at different axons. Otherwise, the color and direction of the arrowheads (upward or downward) are arbitrary. The calibration bar in the lower panel shows 10 μm and applies to all panels.

    Journal: Frontiers in Neuroanatomy

    Article Title: Calcium/calmodulin-dependent protein kinase II associates with the K+ channel isoform Kv4.3 in adult rat optic nerve

    doi: 10.3389/fnana.2022.958986

    Figure Lengend Snippet: Kv4.3 immunopositivity in optic nerve axons. Confocally imaged portion of optic nerve that was longitudinally sectioned and immunostained with antibodies directed against Kv4.3 (upper panel) and NF-70 (middle panel). The upper and middle panels are superimposed in the lower panel (“merge”). The tip of each arrowhead points to an axon (based on NF-70 immunopositivity). Arrowheads of a given color point at the same axon. Different colors are used to point at different axons. Otherwise, the color and direction of the arrowheads (upward or downward) are arbitrary. The calibration bar in the lower panel shows 10 μm and applies to all panels.

    Article Snippet: The anti-CaMKII antibody immunoprecipitated protein that was detectable by anti-CaMKII antibody ( , lane 4), co-immunoprecipitated protein that was detectable by anti-Kv4.3 antibody ( , lane 2), and bound to protein in the elute from the anti-Kv4.3 antibody pull-down ( , lane 5).

    Techniques:

    Control for non-specific binding by rabbit anti-Kv4.3 antibody. (A) Fields of optic nerve that were longitudinally sectioned and processed as in Figure 3 , except that the anti-Kv4.3 antibody in the upper panel was preincubated with immunogen, and the anti-Kv4.3 antibody in the lower panel was not (as in Figure 3 ). These fields were imaged at identical confocal microscope settings (laser intensity, photomultiplier gain, and pinhole diameter). The calibration bar in the lower panel shows 10 μm and applies to both panels. (B) Western-blotted lanes of optic nerve lysate. The lanes were processed identically except that the anti-Kv4.3 antibody was either preincubated with immunogen (in the lane marked “+immunogen”) or it was used without this preincubation (in the lane marked “–immunogen”). To gauge protein load, both lanes were probed with rat anti-myelin basic protein antibody. The fluorescence of the anti-rabbit secondary antibody is pseudo-colored red. The fluorescence of the anti-rat secondary antibody is pseudo-colored blue. The molecular weight and migration distance of protein standards that were run in an adjacent lane are shown along the right edge.

    Journal: Frontiers in Neuroanatomy

    Article Title: Calcium/calmodulin-dependent protein kinase II associates with the K+ channel isoform Kv4.3 in adult rat optic nerve

    doi: 10.3389/fnana.2022.958986

    Figure Lengend Snippet: Control for non-specific binding by rabbit anti-Kv4.3 antibody. (A) Fields of optic nerve that were longitudinally sectioned and processed as in Figure 3 , except that the anti-Kv4.3 antibody in the upper panel was preincubated with immunogen, and the anti-Kv4.3 antibody in the lower panel was not (as in Figure 3 ). These fields were imaged at identical confocal microscope settings (laser intensity, photomultiplier gain, and pinhole diameter). The calibration bar in the lower panel shows 10 μm and applies to both panels. (B) Western-blotted lanes of optic nerve lysate. The lanes were processed identically except that the anti-Kv4.3 antibody was either preincubated with immunogen (in the lane marked “+immunogen”) or it was used without this preincubation (in the lane marked “–immunogen”). To gauge protein load, both lanes were probed with rat anti-myelin basic protein antibody. The fluorescence of the anti-rabbit secondary antibody is pseudo-colored red. The fluorescence of the anti-rat secondary antibody is pseudo-colored blue. The molecular weight and migration distance of protein standards that were run in an adjacent lane are shown along the right edge.

    Article Snippet: The anti-CaMKII antibody immunoprecipitated protein that was detectable by anti-CaMKII antibody ( , lane 4), co-immunoprecipitated protein that was detectable by anti-Kv4.3 antibody ( , lane 2), and bound to protein in the elute from the anti-Kv4.3 antibody pull-down ( , lane 5).

    Techniques: Binding Assay, Microscopy, Western Blot, Fluorescence, Molecular Weight, Migration

    Reciprocal co-immunoprecipitation of Kv4.3 and CaMKII. Western-blotted lanes (1-7), with one lane cut vertically into two parts (lanes 3a, 3b). The material that was loaded into the gel lanes for electrophoresis, and the antibody used to probe each Western-blotted lane, are listed above and below the lanes, respectively. The lanes were loaded with either optic nerve lysate (lanes 3a, 3b, 6), elute from the anti-Kv4.3 antibody pull-down (lanes 1, 5), or elute from the anti-CaMKII antibody pull-down (lanes 2, 4, 7). The Western-blotted lanes were probed with rabbit anti-Kv4.3 antibody (“Kv4.3”) and anti-rabbit secondary antibody, mouse anti-CaMKII antibody (“CaMKII”) and anti-mouse secondary antibody, or anti-rabbit secondary antibody without primary antibody (“2° only”). The molecular weight and migration distance of protein standards that were run in an adjacent lane are shown along the left edge. Note that the faint bands around 60 kDa in lane 3a are absent in lane 3b.

    Journal: Frontiers in Neuroanatomy

    Article Title: Calcium/calmodulin-dependent protein kinase II associates with the K+ channel isoform Kv4.3 in adult rat optic nerve

    doi: 10.3389/fnana.2022.958986

    Figure Lengend Snippet: Reciprocal co-immunoprecipitation of Kv4.3 and CaMKII. Western-blotted lanes (1-7), with one lane cut vertically into two parts (lanes 3a, 3b). The material that was loaded into the gel lanes for electrophoresis, and the antibody used to probe each Western-blotted lane, are listed above and below the lanes, respectively. The lanes were loaded with either optic nerve lysate (lanes 3a, 3b, 6), elute from the anti-Kv4.3 antibody pull-down (lanes 1, 5), or elute from the anti-CaMKII antibody pull-down (lanes 2, 4, 7). The Western-blotted lanes were probed with rabbit anti-Kv4.3 antibody (“Kv4.3”) and anti-rabbit secondary antibody, mouse anti-CaMKII antibody (“CaMKII”) and anti-mouse secondary antibody, or anti-rabbit secondary antibody without primary antibody (“2° only”). The molecular weight and migration distance of protein standards that were run in an adjacent lane are shown along the left edge. Note that the faint bands around 60 kDa in lane 3a are absent in lane 3b.

    Article Snippet: The anti-CaMKII antibody immunoprecipitated protein that was detectable by anti-CaMKII antibody ( , lane 4), co-immunoprecipitated protein that was detectable by anti-Kv4.3 antibody ( , lane 2), and bound to protein in the elute from the anti-Kv4.3 antibody pull-down ( , lane 5).

    Techniques: Immunoprecipitation, Western Blot, Electrophoresis, Molecular Weight, Migration

    Effects of a 24 h treatment with various signaling pathway inhibitors on IL-10 and IL-8 expression and effects of the 24 h treatment with the ERK1/2 inhibitor, SCH772984 and JNK inhibitor, SP600125 on IL-10 and IL-8 secretion in THP-1-derived M 2 macrophages. ( A , D ): Real-time PCR examination of IL-10 ( A ) and IL-8 ( D ) expression in SCH772964 (1 μM)-, SP600125 (10 μM)-, PD169316 (10 μM)-, LY294002 (10 μM)-, AZD5363 (2 μM)-, everolimus (10 nM)-, LY364947 (10 μM)-, ciclosporin A (CsA, 1 μM)-, T-5224 (10 μM)-, and Bay11-7082 (10 μM)-treated THP-1-derived M 2 macrophages for 24 h. Relative mRNA expression in the vehicle control is expressed as 1.0 (n = 4 for each). ( B , C , E , F ): Quantitative detection of IL-10 ( B , C ) and/or IL-8 ( E , F ) secretion by an ELISA assay in SCH772964 ( B , E )- and SP600125 ( C , F )-treated THP-1-derived M 2 macrophages. Relative cytokine secretion in the vehicle control is expressed as 1.0 (n = 4 for each). **: p

    Journal: International Journal of Molecular Sciences

    Article Title: Downregulation of IL-8 and IL-10 by the Activation of Ca2+-Activated K+ Channel KCa3.1 in THP-1-Derived M2 Macrophages

    doi: 10.3390/ijms23158603

    Figure Lengend Snippet: Effects of a 24 h treatment with various signaling pathway inhibitors on IL-10 and IL-8 expression and effects of the 24 h treatment with the ERK1/2 inhibitor, SCH772984 and JNK inhibitor, SP600125 on IL-10 and IL-8 secretion in THP-1-derived M 2 macrophages. ( A , D ): Real-time PCR examination of IL-10 ( A ) and IL-8 ( D ) expression in SCH772964 (1 μM)-, SP600125 (10 μM)-, PD169316 (10 μM)-, LY294002 (10 μM)-, AZD5363 (2 μM)-, everolimus (10 nM)-, LY364947 (10 μM)-, ciclosporin A (CsA, 1 μM)-, T-5224 (10 μM)-, and Bay11-7082 (10 μM)-treated THP-1-derived M 2 macrophages for 24 h. Relative mRNA expression in the vehicle control is expressed as 1.0 (n = 4 for each). ( B , C , E , F ): Quantitative detection of IL-10 ( B , C ) and/or IL-8 ( E , F ) secretion by an ELISA assay in SCH772964 ( B , E )- and SP600125 ( C , F )-treated THP-1-derived M 2 macrophages. Relative cytokine secretion in the vehicle control is expressed as 1.0 (n = 4 for each). **: p

    Article Snippet: Primary antibodies against KCa 3.1 (rabbit polyclonal), ERK1/2 (rabbit polyclonal), phospho-ERK1(T202/Y204)/ERK2(T185/Y187) (rabbit monoclonal), JNK (rabbit polyclonal), phospho-JNK(Y185) (rabbit polyclonal), c-Jun (rabbit polyclonal), CREB (rabbit polyclonal), phospho-CREB(S133) (rabbit polyclonal), and β-actin (ACTB) (mouse monoclonal) were from Alomone Labs (Jerusalem, Israel), BioLegend (San Diego, CA, USA), R & D Systems (Minneapolis, MN, USA), GeneTex (Alton Pkwy Irvine, CA, USA), ProteinTech (Rosemont, IL, USA), Medical & Biological Laboratories (Nagoya, Japan), and ABclonal (Tokyo, Japan).

    Techniques: Expressing, Derivative Assay, Real-time Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay

    Protein expression levels of phosphorylated ERK1/2 (P-ERK1/2) and P-JNK in THP-1-derived M 2 macrophages following the SKA-121 treatment and high [K + ] e exposure. A – D : Western blot showing P-ERK1/2, total ERK1/2 (ERK1/2) ( A,B ), P-JNK, and total JNK (JNK) ( C , D ) in 1 μM SKA-121-treated ( A , C ) and high [K + ] e -exposed ( B , D ) THP-1-derived M 2 macrophages. Specific band signals were observed at 42 (P-ERK2), 42 (ERK2), 43/50 (P-JNK), and 43/50 (JNK) kDa. E – H : Summarized results of the relative protein expression of P-ERK2/ERK2 ( E , F ) and P-JNK/JNK ( G , H ) in 1 μM SKA-121-treated ( E , G ) and high [K + ] e -exposed ( F , H ) THP-1-derived M 2 macrophages. After compensation with the optical density of the ACTB signal (43 kDa), the expression level in the vehicle control or 5 mM K + is expressed as 1.0 (n = 4 for each). **: p

    Journal: International Journal of Molecular Sciences

    Article Title: Downregulation of IL-8 and IL-10 by the Activation of Ca2+-Activated K+ Channel KCa3.1 in THP-1-Derived M2 Macrophages

    doi: 10.3390/ijms23158603

    Figure Lengend Snippet: Protein expression levels of phosphorylated ERK1/2 (P-ERK1/2) and P-JNK in THP-1-derived M 2 macrophages following the SKA-121 treatment and high [K + ] e exposure. A – D : Western blot showing P-ERK1/2, total ERK1/2 (ERK1/2) ( A,B ), P-JNK, and total JNK (JNK) ( C , D ) in 1 μM SKA-121-treated ( A , C ) and high [K + ] e -exposed ( B , D ) THP-1-derived M 2 macrophages. Specific band signals were observed at 42 (P-ERK2), 42 (ERK2), 43/50 (P-JNK), and 43/50 (JNK) kDa. E – H : Summarized results of the relative protein expression of P-ERK2/ERK2 ( E , F ) and P-JNK/JNK ( G , H ) in 1 μM SKA-121-treated ( E , G ) and high [K + ] e -exposed ( F , H ) THP-1-derived M 2 macrophages. After compensation with the optical density of the ACTB signal (43 kDa), the expression level in the vehicle control or 5 mM K + is expressed as 1.0 (n = 4 for each). **: p

    Article Snippet: Primary antibodies against KCa 3.1 (rabbit polyclonal), ERK1/2 (rabbit polyclonal), phospho-ERK1(T202/Y204)/ERK2(T185/Y187) (rabbit monoclonal), JNK (rabbit polyclonal), phospho-JNK(Y185) (rabbit polyclonal), c-Jun (rabbit polyclonal), CREB (rabbit polyclonal), phospho-CREB(S133) (rabbit polyclonal), and β-actin (ACTB) (mouse monoclonal) were from Alomone Labs (Jerusalem, Israel), BioLegend (San Diego, CA, USA), R & D Systems (Minneapolis, MN, USA), GeneTex (Alton Pkwy Irvine, CA, USA), ProteinTech (Rosemont, IL, USA), Medical & Biological Laboratories (Nagoya, Japan), and ABclonal (Tokyo, Japan).

    Techniques: Expressing, Derivative Assay, Western Blot

    Expression of Kv1.3 in human pancreatic cancer tissue. Expression of Kv1.3 in human pancreatic tissue. Panel ( a ), high expression level of Kv1.3 in human pancreatic cancer tissue. ( n = 33 out of 55, scale bar = 50 µm). Panel ( b ), magnification of panel ( a ), scale bar = 20 µm. Panel ( c ), low expression level of Kv1.3 in human pancreatic cancer tissue. ( n = 22 out of 55, scale bar = 50 µm). Panel ( d ), magnification of panel c, scale bar = 20 µm. Panel ( e ), Immunoglobulin G (IgG) control with magnification (Panel ( f )). Panel ( g ) shows a pancreas adenocarcinoma ductule with high expression of Kv1.3 (brown color) and adjacent normal pancreas parenchyma without Kv1.3 expression (black arrow), scale bar = 50 µm. Panel ( h ), magnification of panel ( g ), scale bar = 20 µm.

    Journal: Cancers

    Article Title: Inhibition of a Mitochondrial Potassium Channel in Combination with Gemcitabine and Abraxane Drastically Reduces Pancreatic Ductal Adenocarcinoma in an Immunocompetent Orthotopic Murine Model

    doi: 10.3390/cancers14112618

    Figure Lengend Snippet: Expression of Kv1.3 in human pancreatic cancer tissue. Expression of Kv1.3 in human pancreatic tissue. Panel ( a ), high expression level of Kv1.3 in human pancreatic cancer tissue. ( n = 33 out of 55, scale bar = 50 µm). Panel ( b ), magnification of panel ( a ), scale bar = 20 µm. Panel ( c ), low expression level of Kv1.3 in human pancreatic cancer tissue. ( n = 22 out of 55, scale bar = 50 µm). Panel ( d ), magnification of panel c, scale bar = 20 µm. Panel ( e ), Immunoglobulin G (IgG) control with magnification (Panel ( f )). Panel ( g ) shows a pancreas adenocarcinoma ductule with high expression of Kv1.3 (brown color) and adjacent normal pancreas parenchyma without Kv1.3 expression (black arrow), scale bar = 50 µm. Panel ( h ), magnification of panel ( g ), scale bar = 20 µm.

    Article Snippet: Primary antibodies Kv1.3 (1:100, APC-101, Alomone labs, Jerusalem, Israel) and TOM20 (1:100, MABT166, Sigma, Burlington, MA, USA) were incubated overnight at 4 °C.

    Techniques: Expressing

    Analysis of mRNA levels and the expression of different VDCC subtypes in adrenal medullae of WT and 3xTg mice over 12 m age. ( A ) Normalized mRNA levels of different VDCCs in adrenal medullae from WT and 3xTg mice > 12 m. Both medullas of the same mouse were homogenized, so each dot represents an individual mouse. ( B ) Representative image of WT and 3xTg adrenal medulla stained with anti-Cav1.3 (top) and anti Cav2.1 (bottom) + DAPI (nuclei). ( C ) IntDen quantification of calcium channel expression. Each dot represents an image analyzed, and the bars represent mean ± SEM. A Student’s t -test was used to statistically compare the WT and 3xTg groups. ** p

    Journal: Biology

    Article Title: Alterations of the Sympathoadrenal Axis Related to the Development of Alzheimer’s Disease in the 3xTg Mouse Model

    doi: 10.3390/biology11040511

    Figure Lengend Snippet: Analysis of mRNA levels and the expression of different VDCC subtypes in adrenal medullae of WT and 3xTg mice over 12 m age. ( A ) Normalized mRNA levels of different VDCCs in adrenal medullae from WT and 3xTg mice > 12 m. Both medullas of the same mouse were homogenized, so each dot represents an individual mouse. ( B ) Representative image of WT and 3xTg adrenal medulla stained with anti-Cav1.3 (top) and anti Cav2.1 (bottom) + DAPI (nuclei). ( C ) IntDen quantification of calcium channel expression. Each dot represents an image analyzed, and the bars represent mean ± SEM. A Student’s t -test was used to statistically compare the WT and 3xTg groups. ** p

    Article Snippet: After the generous washing of the tissues with PBS and the blocking of the tissues with goat serum, the slices were incubated overnight with primary antibodies—antiCav1.3 (1:200; ACC-003; Alomone Labs; Jerusalem, Israel) and antiCav2.1 (1:200; ACC-001; Alomone labs)—and then with secondary antibodies (1:500).

    Techniques: Expressing, Mouse Assay, Staining