Journal: PLoS ONE

Article Title: Systemic Transplantation of Human Adipose Tissue-Derived Mesenchymal Stem Cells for the Regeneration of Irradiation-Induced Salivary Gland Damage

doi: 10.1371/journal.pone.0071167

Figure Lengend Snippet: FISH revealed cells reactive to human specific Alu DNA (green, arrow) in irradiated SGs. A few Alu-positive cells were also positive for the acinar cell marker, α-amylase (red). At least three random tissue sections per gland from ten mice were chosen for evaluation. DAPI (blue), Bar = 20 µm.

Article Snippet: Primary polyclonal anti-α-amylase (1∶100, Cell Signaling) antibodies were used to test the functionality of acinar cells and the differentiation of hAdMSCs into SGCs.

Techniques: Irradiation, Marker

Journal: PLoS ONE

Article Title: Systemic Transplantation of Human Adipose Tissue-Derived Mesenchymal Stem Cells for the Regeneration of Irradiation-Induced Salivary Gland Damage

doi: 10.1371/journal.pone.0071167

Figure Lengend Snippet: (A) Approximately 13%–18% of hAdMSCs co-cultured in the upper chamber of a Transwell co-culture system for 5 days stained positively for α-amylase (red). AdMSCs cultured alone only showed the nuclear expression of hAdMSCs (blue). Bar = 50 µm (B) Reverse transcription-polymerase chain reaction analysis confirmed α-AMY gene expression from the cocultured hAdMSCs, but no band was seen in the hAdMSC control. Human SGCs were used as a positive control.

Article Snippet: Primary polyclonal anti-α-amylase (1∶100, Cell Signaling) antibodies were used to test the functionality of acinar cells and the differentiation of hAdMSCs into SGCs.

Techniques: Cell Culture, Co-Culture Assay, Staining, Expressing, Reverse Transcription Polymerase Chain Reaction, Positive Control

Journal: Computational and Structural Biotechnology Journal

Article Title: Bulk RNA-seq and scRNA-seq analysis reveal an activation of immune response and compromise of secretory function in major salivary glands of obese mice

doi: 10.1016/j.csbj.2022.11.054

Figure Lengend Snippet: The levels of immune response related proteins were elevated in the SGs of DIO mice. (A-C) Immunohistochemical analysis of CD4 (A), CD8 (B) and CD79A (C) in the SMG, SLG and PG from the NC and DIO mice (week-35), respectively. Arrow points to the representative region with elevated protein positive signals in the DIO mice. (D) Western blot analysis of CD4, CD79A, CD8A, MUC20, MUC13 and α-Amylase in the SGs of three NC mice and three DIO mice (week-35), respectively. β-Actin: loading control.

Article Snippet: Antibodies against CD4 (ER1706-80, HuaBio, Hangzhou), CD8A (0108–7, HuaBio, Hangzhou), CD79A (EM1902-29, HuaBio, Hangzhou), MUC13 (ER1913-37, HuaBio, Hangzhou), MUC20 (A15968, ABclonal,Wuhan), α-Amylase (3796,Cell Signaling Technology, America) andβ-Actin (AC026, ABclonal, Wuhan) were purchased.

Techniques: Immunohistochemical staining, Western Blot

Journal: Computational and Structural Biotechnology Journal

Article Title: Bulk RNA-seq and scRNA-seq analysis reveal an activation of immune response and compromise of secretory function in major salivary glands of obese mice

doi: 10.1016/j.csbj.2022.11.054

Figure Lengend Snippet: SGs of the DIO mice produced less mucin. (A-C) Images showing the alcian blue staining of sections of the SMG (A), SLG (B) and PG (C) dissected from NC-2, NC-3, NC-7, NC-8, DIO-2 and DIO-3, DIO-7 or DIO-8 as indicated. Statistic comparison of the relative staining intensity (ratio of the blue color pixels against total color pixels) between NC and DIO mice is shown on the right. Blue staining signals of 2 sections from each NC (NC-2, NC-3, NC-7, and NC-8) and DIO (DIO-2, DIO-3, DIO-7 and DIO-8) mice were acquired for statistic analysis. (D-F) Comparing the pilocarpine hydrochloride stimulated saliva flow rate (n = 3) (D), peroxidase (POD) activity (n = 3) (E) and proteins levels of MUC13 and α-Amylase in the stimulated saliva between three NC and three DIO mice (week-35). For (F), equal amount of the loaded protein samples was determined based on the protein concentration measured using the Bradford method. ***, p < 0.001; ****, p < 0.0001.

Article Snippet: Antibodies against CD4 (ER1706-80, HuaBio, Hangzhou), CD8A (0108–7, HuaBio, Hangzhou), CD79A (EM1902-29, HuaBio, Hangzhou), MUC13 (ER1913-37, HuaBio, Hangzhou), MUC20 (A15968, ABclonal,Wuhan), α-Amylase (3796,Cell Signaling Technology, America) andβ-Actin (AC026, ABclonal, Wuhan) were purchased.

Techniques: Produced, Staining, Activity Assay, Protein Concentration

Journal: PLoS ONE

Article Title: The Zinc Transporter Zip5 ( Slc39a5 ) Regulates Intestinal Zinc Excretion and Protects the Pancreas against Zinc Toxicity

doi: 10.1371/journal.pone.0082149

Figure Lengend Snippet: Two weeks after the last tamoxifen injection pancreas-specific Zip5 -knockout ( Zip5 Panc KO ) mice were given an I.P. injection of zinc sulfate (12.5 mg zinc/kg body weight) and 24 hr later pancreata were harvested and paraffin sections were prepared and stained for α-amylase using immunohistochemistry. Sections from two different mice are shown. Dark brown deposits in acinar cells indicate positive staining. Black arrowheads demarcate large cytoplasmic vacuoles containing α-amylase. Sections were photographed at 630× magnification. I ; indicates an Islet of Langerhans.

Article Snippet: Sections were incubated with antisera against ZIP5 (1∶200), as described previously , after antigen retrieval in citrate or sections were incubated with antisera against α-amylase (1∶300; Cell Signaling).

Techniques: Injection, Knock-Out, Staining, Immunohistochemistry