anti β actin (Millipore)
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Anti β Actin, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti β actin/product/Millipore
Average 99 stars, based on 1 article reviews
Price from $9.99 to $1999.99
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1) Product Images from "Inactivation of PRIM1 Function Sensitizes Cancer Cells to ATR and CHK1 Inhibitors"
Article Title: Inactivation of PRIM1 Function Sensitizes Cancer Cells to ATR and CHK1 Inhibitors
Journal: Neoplasia (New York, N.Y.)
doi: 10.1016/j.neo.2018.08.009

Figure Legend Snippet: siPRIM1-mediated effects on cell cycle progression in ATR s/s cells. (A) Cell cycle profile of DLD-1 ATR +/+ and ATR s/s cells 144 hours after siPRIM1 transfection as assessed by FACS analysis. (B) S-phase fraction of the cell cycle profile in detail. (C and D) Protein quantification in DLD-1 ATR +/+ and ATR s/s cells by immunoblotting 144 hours after siPRIM1 transfection. β-Actin was used as loading control. Percentage protein change of PRIM1 -depleted cells was normalized to NTC of DLD-1 ATR +/+ and ATR s/s cells. Error bars represent ±SEM of three independent experiments. Asterisks mark statistical significance using a two-tailed, unpaired Student's t test (* P
Techniques Used: Transfection, FACS, Two Tailed Test

Figure Legend Snippet: siPRIM1-mediated sensitization of DLD-1 cells to ATR and CHK1 inhibitors. (A) Knockdown efficiency of PRIM1 via siRNA was confirmed by immunoblotting 96 hours after transfection. β-Actin was used as loading control. (B) Effects of ATR inhibitors, (C) CHK1 inhibitors, and (D) common chemotherapeutics on the proliferation of PRIM1 -depleted DLD-1 cells as compared to control and mock-transfected cells measured 120 hours after drug treatment. Error bars represent ±SEM of three independent experiments with each data point reflecting triplicate wells. Asterisks mark statistical significance using a two-tailed, unpaired Student's t test (* P
Techniques Used: Transfection, Two Tailed Test

Figure Legend Snippet: Verification of synthetic lethality between ATR and PRIM1 . (A) Quantification of ATR protein in DLD-1 ATR +/+ , ATR s/s , and ATR resc cells by immunoblotting. β-Actin was used as loading control. (B) MMC sensitivity was assessed 120 hours after treatment by proliferation assay in DLD-1 ATR +/+ , ATR s/s , and ATR resc cells. (C) Quantification of siRNA-mediated PRIM1 depletion (10 nM) 120 hours after transfection by immunoblotting. β-Actin was used as loading control. (D) Proliferation inhibition was assessed 144 hours after transfection by proliferation assay in DLD-1 ATR +/+ , ATR s/s , and ATR resc cells. Error bars represent ±SEM of three independent experiments with each data point reflecting triplicate wells. Asterisks mark statistical significance using a two-tailed, unpaired Student's t test (* P
Techniques Used: Proliferation Assay, Transfection, Inhibition, Two Tailed Test

Figure Legend Snippet: siPRIM1-mediated sensitization to ATR and CHK1 inhibitors in a panel of different cancer cell lines. (A) Knockdown efficiency of PRIM1 via siRNA was confirmed by immunoblotting 96 hours after transfection. β-Actin was used as loading control. (B) Effects of ATR and (C) CHK1 inhibitors on the proliferation of PRIM1 -depleted SW480, RKO, and PaTu 8988t cells as compared to control and mock-transfected cells measured 120 hours after drug treatment. Error bars represent ±SEM of at least three independent experiments with each data point reflecting triplicate wells. Asterisks mark statistical significance using a two-tailed, unpaired Student's t test (* P
Techniques Used: Transfection, Two Tailed Test
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