anti β actin  (Cell Signaling Technology Inc)

 
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    Name:
    β Actin 13E5 Rabbit mAb Alexa Fluor 555 Conjugate
    Description:
    This Cell Signaling Technology antibody is conjugated to Alexa Fluor 555 fluorescent dye and tested in house for immunofluorescent analysis in monkey cells The antibody is expected to exhibit the same species cross reactivity as the unconjugated β Actin 13E5 Rabbit mAb 4970
    Catalog Number:
    8046
    Price:
    None
    Category:
    Antibody Conjugates
    Source:
    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino-terminus of human β-actin protein.
    Reactivity:
    Human Mouse Rat Monkey Bovine Pig
    Applications:
    Immunofluorescence
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    Structured Review

    Cell Signaling Technology Inc anti β actin
    This Cell Signaling Technology antibody is conjugated to Alexa Fluor 555 fluorescent dye and tested in house for immunofluorescent analysis in monkey cells The antibody is expected to exhibit the same species cross reactivity as the unconjugated β Actin 13E5 Rabbit mAb 4970
    https://www.bioz.com/result/anti β actin/product/Cell Signaling Technology Inc
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti β actin - by Bioz Stars, 2021-03
    93/100 stars

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    Related Articles

    Labeling:

    Article Title: The Antitumor Effect of Xihuang Pill on Treg Cells Decreased in Tumor Microenvironment of 4T1 Breast Tumor-Bearing Mice by PI3K/AKT~AP-1 Signaling Pathway
    Article Snippet: .. The above antibodies were as follows: primary antibodies: Rabbit anti-mouse β -actin, Rabbit anti-mouse PI3K P110α (1 : 1000, CST, USA), Rabbit anti-mouse C-JUN antibody, Rabbit anti-mouse AKT, and Rabbit anti-mouse PI3K P85α (1 : 1000, Abcam, USA); secondary fluorescently labeled antibodies: Donkey anti-rabbit antibody (Alexa Fluor 790) and Donkey anti-rabbit (Alexa Fluor 680) (both 1 : 10000, Abcam, USA). .. The results were tested by Odyssey CLX S/N CLX-0926 (LICOR, USA).

    Article Title: The Antitumor Effect of Xihuang Pill on Treg Cells Decreased in Tumor Microenvironment of 4T1 Breast Tumor-Bearing Mice by PI3K/AKT~AP-1 Signaling Pathway
    Article Snippet: .. The above antibodies were as follows: primary antibodies: Rabbit anti-mouse β -actin, Rabbit anti-mouse PI3K P110α (all 1 : 100, CST, USA), Rabbit anti-mouse C-JUN antibody, Rabbit anti-mouse AKT, and Rabbit anti-mouse PI3K P85α (all 1 : 100, Abcam, USA); secondary fluorescently labeled antibodies: Donkey anti-rabbit antibody (Alexa Fluor 488) and Donkey anti-rabbit antibody (Cy3) (all 1 : 200, Jackson, USA). .. Testing equipment was Olympus IX73 microscope (Tokyo, Japan).

    Article Title: Magnesium ion influx reduces neuroinflammation in Aβ precursor protein/Presenilin 1 transgenic mice by suppressing the expression of interleukin-1β
    Article Snippet: .. Antibodies against β-actin, ERK1/2, p-ERK1/2 (Thr 202/Tyr 204), PPARγ, Aβ, IL-1β, NeuN, glial fibrillary acidic protein (GFAP), Alexa Fluor 488-labeled, Fluor 555-labeled, and horseradish peroxidase -labeled secondary antibody were purchased from Cell Signaling Technology, Inc. (Danvers, MA, USA). .. Iba-1, p-PPARγ (Ser 82) antibody was from Merck Millipore (Bedford, MA, USA).

    Article Title: The Antitumor Effect of Xihuang Pill on Treg Cells Decreased in Tumor Microenvironment of 4T1 Breast Tumor-Bearing Mice by PI3K/AKT~AP-1 Signaling Pathway
    Article Snippet: .. The above antibodies were as follows: primary antibodies: Rabbit anti-mouse β -actin, Rabbit anti-mouse PI3K P110 α (1 : 1000, CST, USA), Rabbit anti-mouse C-JUN antibody, Rabbit anti-mouse AKT, and Rabbit anti-mouse PI3K P85 α (1 : 1000, Abcam, USA); secondary fluorescently labeled antibodies: Donkey anti-rabbit antibody (Alexa Fluor 790) and Donkey anti-rabbit (Alexa Fluor 680) (both 1 : 10000, Abcam, USA). .. The results were tested by Odyssey CLX S/N CLX-0926 (LICOR, USA).

    other:

    Article Title: Pharmacological Ascorbate Suppresses Growth of Gastric Cancer Cells with GLUT1 Overexpression and Enhances the Efficacy of Oxaliplatin Through Redox Modulation
    Article Snippet: Antibodies used for immunoblotting include anti-γ-H2 AX, anti-β-Actin, anti-cleaved caspase 3 (Cell Signaling Technology, Beverly, MA, USA), anti-GLUT1, Alexa Fluor® 647-conjugated anti-GLUT1, anti-GLUT3, anti-GLUT4, anti-SVCT1, anti-SVCT2, anti-Na+ /K+ ATPase, anti-Vinculin and anti-Ki67 (Abcam, Cambridge, Massachusetts, USA).

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    Cell Signaling Technology Inc rabbit anti β actin
    Genetic ablation of Nrf2 reduces angiogenic sprouting and vascular density. ( A ) Blood vessels were visualized by PECAM-1 staining of retinas at P5. Decreased vascular density ( B ), branch points ( C ), sprouts ( D ), and filopodia ( E ) were observed in Nrf2 − / − retina ( n = 6). ( F and G ) Isolectin B4 (green) and BrdU labeling (red) of retinas at P5 demonstrate reduced EC proliferation in Nrf2 − / − ( n = 4). (Scale bar, 50 µm.) ( H ) NG2 + pericytes in capillaries or SMA + vascular smooth muscle cells in arterioles were indistinguishable between WT ( Nrf2 +/+ ) and Nrf2 − / − retinas at P5. (Scale bar, 100 µm.) ( I ) Nrf2 is expressed in developing blood vessels, and strong expression was observed in the sprouting vascular front. Arrowheads indicate Nrf2 expression in tip cells and stalk cells (A, artery; V, vein). ( J ) Nrf2 expression was increased in the ganglion cell layer (GCL) of retina at P5 compared with P0. Nrf2 was highly expressed in the vessels and surrounding area (arrowheads). Nrf2 localization in the nucleus was apparent (arrows). HV, hyaloid vessels; INL, inner nuclear layer. ( K ) Immunoblot analysis of Nrf2 in the retinas of WT mice at P0 and P5. <t>β-Actin</t> was used as a loading control ( n = 3). ( L ) Quantitative RT-PCR analysis of Nrf2 and Nrf2 target genes in the retinas of WT mice at P0 and P5 ( n = 5). Data are presented as mean ± SEM (** P
    Rabbit Anti β Actin, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti β actin/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti β actin - by Bioz Stars, 2021-03
    86/100 stars
      Buy from Supplier

    97
    Cell Signaling Technology Inc mouse anti β actin
    Downregulation of BKCa inhibits prostate cancer cell proliferation, migration and invasion A. Prostate cancer PC3 and C4-2 cells were stably infected with BKCa-shRNA (BK-shRNA) or Scrambled-shRNA (SC-shRNA). The expression of BKCa was analyzed by western blot. <t>β-actin</t> was used as loading control. B. Cell growth curves as determined by MTT assay. C. Percentage of EdU positive cells determined by EdU incorporation assay (lower panel) and representative images of EdU staining (upper panel) in PC3 and C4-2 cells. D. Effects of downregulation of BKCa on the colony-genic ability of PC3 and C4-2 cells. Representative images were shown in the upper panel and the number of colonies was illustrated in the lower panel. E. Reduced expression of BKCa inhibits prostate cancer cell migration as determined by scratch wound healing assay. The representative images of wound healing were shown in left panel. The relative wound closure was illustrated in right panel. F-H. Effects of downregulated BKCa on the migration and invasion of PC3 and C4-2 cells as determined by transwell assays. Representative images and mean numbers of migrated (G) and invaded (H) cells were shown in the upper and lower panel respectively. All the experiments were performed in triplicate. The data are shown as the means ± se. *P
    Mouse Anti β Actin, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse anti β actin/product/Cell Signaling Technology Inc
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse anti β actin - by Bioz Stars, 2021-03
    97/100 stars
      Buy from Supplier

    Image Search Results


    Genetic ablation of Nrf2 reduces angiogenic sprouting and vascular density. ( A ) Blood vessels were visualized by PECAM-1 staining of retinas at P5. Decreased vascular density ( B ), branch points ( C ), sprouts ( D ), and filopodia ( E ) were observed in Nrf2 − / − retina ( n = 6). ( F and G ) Isolectin B4 (green) and BrdU labeling (red) of retinas at P5 demonstrate reduced EC proliferation in Nrf2 − / − ( n = 4). (Scale bar, 50 µm.) ( H ) NG2 + pericytes in capillaries or SMA + vascular smooth muscle cells in arterioles were indistinguishable between WT ( Nrf2 +/+ ) and Nrf2 − / − retinas at P5. (Scale bar, 100 µm.) ( I ) Nrf2 is expressed in developing blood vessels, and strong expression was observed in the sprouting vascular front. Arrowheads indicate Nrf2 expression in tip cells and stalk cells (A, artery; V, vein). ( J ) Nrf2 expression was increased in the ganglion cell layer (GCL) of retina at P5 compared with P0. Nrf2 was highly expressed in the vessels and surrounding area (arrowheads). Nrf2 localization in the nucleus was apparent (arrows). HV, hyaloid vessels; INL, inner nuclear layer. ( K ) Immunoblot analysis of Nrf2 in the retinas of WT mice at P0 and P5. β-Actin was used as a loading control ( n = 3). ( L ) Quantitative RT-PCR analysis of Nrf2 and Nrf2 target genes in the retinas of WT mice at P0 and P5 ( n = 5). Data are presented as mean ± SEM (** P

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Nrf2 acts cell-autonomously in endothelium to regulate tip cell formation and vascular branching

    doi: 10.1073/pnas.1309276110

    Figure Lengend Snippet: Genetic ablation of Nrf2 reduces angiogenic sprouting and vascular density. ( A ) Blood vessels were visualized by PECAM-1 staining of retinas at P5. Decreased vascular density ( B ), branch points ( C ), sprouts ( D ), and filopodia ( E ) were observed in Nrf2 − / − retina ( n = 6). ( F and G ) Isolectin B4 (green) and BrdU labeling (red) of retinas at P5 demonstrate reduced EC proliferation in Nrf2 − / − ( n = 4). (Scale bar, 50 µm.) ( H ) NG2 + pericytes in capillaries or SMA + vascular smooth muscle cells in arterioles were indistinguishable between WT ( Nrf2 +/+ ) and Nrf2 − / − retinas at P5. (Scale bar, 100 µm.) ( I ) Nrf2 is expressed in developing blood vessels, and strong expression was observed in the sprouting vascular front. Arrowheads indicate Nrf2 expression in tip cells and stalk cells (A, artery; V, vein). ( J ) Nrf2 expression was increased in the ganglion cell layer (GCL) of retina at P5 compared with P0. Nrf2 was highly expressed in the vessels and surrounding area (arrowheads). Nrf2 localization in the nucleus was apparent (arrows). HV, hyaloid vessels; INL, inner nuclear layer. ( K ) Immunoblot analysis of Nrf2 in the retinas of WT mice at P0 and P5. β-Actin was used as a loading control ( n = 3). ( L ) Quantitative RT-PCR analysis of Nrf2 and Nrf2 target genes in the retinas of WT mice at P0 and P5 ( n = 5). Data are presented as mean ± SEM (** P

    Article Snippet: The following antibodies were used: rabbit anti-Nrf2, mouse anti-GAPDH (Abcam), rabbit anti–β-actin, rabbit anti-NICD, rabbit anti–phospho-Akt, mouse anti-Akt1, mouse anti–phospho-p44/42 MAPK (Erk1/2), rabbit anti-p44/42 MAPK (Erk1/2), and rabbit anti-Dll4 (Cell Signaling Technology).

    Techniques: Staining, Labeling, Expressing, Mouse Assay, Quantitative RT-PCR

    Western blot analysis of poly (adenosine diphosphate-ribose) polymerase in A549, PC3 and H1299 cell lines. β-actin was used as loading control. The treatment was conducted for 24 h. Vertical and horizontal lanes indicate separate blots. PQE, Pelargonium quercetorum extract; PARP, poly (adenosine diphosphate-ribose) polymerase.

    Journal: Oncology Letters

    Article Title: Pelargonium quercetorum Agnew induces apoptosis without PARP or cytokeratin 18 cleavage in non-small cell lung cancer cell lines

    doi: 10.3892/ol.2016.4779

    Figure Lengend Snippet: Western blot analysis of poly (adenosine diphosphate-ribose) polymerase in A549, PC3 and H1299 cell lines. β-actin was used as loading control. The treatment was conducted for 24 h. Vertical and horizontal lanes indicate separate blots. PQE, Pelargonium quercetorum extract; PARP, poly (adenosine diphosphate-ribose) polymerase.

    Article Snippet: Western blotting was performed using rabbit anti-β-actin and anti-PARP monoclonal antibodies at 1:1,000 dilution (catalog nos., 4970 and 9532, respectively; Cell Signaling Technology, Inc., Danvers, MA, USA) in 5% (w/v) bovine serum albumin (Amresco, LLC, Solon, OH, USA).

    Techniques: Western Blot

    Downregulation of BKCa inhibits prostate cancer cell proliferation, migration and invasion A. Prostate cancer PC3 and C4-2 cells were stably infected with BKCa-shRNA (BK-shRNA) or Scrambled-shRNA (SC-shRNA). The expression of BKCa was analyzed by western blot. β-actin was used as loading control. B. Cell growth curves as determined by MTT assay. C. Percentage of EdU positive cells determined by EdU incorporation assay (lower panel) and representative images of EdU staining (upper panel) in PC3 and C4-2 cells. D. Effects of downregulation of BKCa on the colony-genic ability of PC3 and C4-2 cells. Representative images were shown in the upper panel and the number of colonies was illustrated in the lower panel. E. Reduced expression of BKCa inhibits prostate cancer cell migration as determined by scratch wound healing assay. The representative images of wound healing were shown in left panel. The relative wound closure was illustrated in right panel. F-H. Effects of downregulated BKCa on the migration and invasion of PC3 and C4-2 cells as determined by transwell assays. Representative images and mean numbers of migrated (G) and invaded (H) cells were shown in the upper and lower panel respectively. All the experiments were performed in triplicate. The data are shown as the means ± se. *P

    Journal: Oncotarget

    Article Title: BKCa promotes growth and metastasis of prostate cancer through facilitating the coupling between αvβ3 integrin and FAK

    doi: 10.18632/oncotarget.9559

    Figure Lengend Snippet: Downregulation of BKCa inhibits prostate cancer cell proliferation, migration and invasion A. Prostate cancer PC3 and C4-2 cells were stably infected with BKCa-shRNA (BK-shRNA) or Scrambled-shRNA (SC-shRNA). The expression of BKCa was analyzed by western blot. β-actin was used as loading control. B. Cell growth curves as determined by MTT assay. C. Percentage of EdU positive cells determined by EdU incorporation assay (lower panel) and representative images of EdU staining (upper panel) in PC3 and C4-2 cells. D. Effects of downregulation of BKCa on the colony-genic ability of PC3 and C4-2 cells. Representative images were shown in the upper panel and the number of colonies was illustrated in the lower panel. E. Reduced expression of BKCa inhibits prostate cancer cell migration as determined by scratch wound healing assay. The representative images of wound healing were shown in left panel. The relative wound closure was illustrated in right panel. F-H. Effects of downregulated BKCa on the migration and invasion of PC3 and C4-2 cells as determined by transwell assays. Representative images and mean numbers of migrated (G) and invaded (H) cells were shown in the upper and lower panel respectively. All the experiments were performed in triplicate. The data are shown as the means ± se. *P

    Article Snippet: The following antibodies were used: rabbit anti-FAK, rabbit anti-integrin αv and β3, mouse anti-β-actin, rabbit anti-pTyr100, peroxidase-conjugated goat anti-rabbit or anti-mouse IgG (all from Cell Signaling Technology, MA, USA) and rabbit anti-BKCa (Alomone Labs, Jerusalem, Israel).

    Techniques: Migration, Stable Transfection, Infection, shRNA, Expressing, Western Blot, MTT Assay, Staining, Wound Healing Assay

    Characterization of the integrin αvβ3/BKCa complex in prostate cancer PC3 cells A. Immunocolocalization of BKCa and αvβ3. Representative confocal images of BKCa (red) and integrin αvβ3 (green) staining conducted in PC3 cells. Staining is more pronounced on the plasma membrane for both proteins and the merge shows that there is a co-localization (yellow to orange areas) on plasma membrane areas. B. Co-immunoprecipitation of integrin αvβ3 and BKCa in PC3 cells. Cells were transfected with BKCa or BK-shRNA and their corresponding negative control vectors. Proteins were extracted and immunoprecipitated using anti-BKCa or anti-αvβ3 antibodies; blots were probed with anti-αvβ3 or anti-BKCa antibodies respectively. Bead lanes contain the protein G conjugated sepharose beads used during the immunoprecipitation without the protein input. Equal amount of protein extract from each group was subjected to Western blot with β-actin as the loading control. All the experiments were performed in triplicate.

    Journal: Oncotarget

    Article Title: BKCa promotes growth and metastasis of prostate cancer through facilitating the coupling between αvβ3 integrin and FAK

    doi: 10.18632/oncotarget.9559

    Figure Lengend Snippet: Characterization of the integrin αvβ3/BKCa complex in prostate cancer PC3 cells A. Immunocolocalization of BKCa and αvβ3. Representative confocal images of BKCa (red) and integrin αvβ3 (green) staining conducted in PC3 cells. Staining is more pronounced on the plasma membrane for both proteins and the merge shows that there is a co-localization (yellow to orange areas) on plasma membrane areas. B. Co-immunoprecipitation of integrin αvβ3 and BKCa in PC3 cells. Cells were transfected with BKCa or BK-shRNA and their corresponding negative control vectors. Proteins were extracted and immunoprecipitated using anti-BKCa or anti-αvβ3 antibodies; blots were probed with anti-αvβ3 or anti-BKCa antibodies respectively. Bead lanes contain the protein G conjugated sepharose beads used during the immunoprecipitation without the protein input. Equal amount of protein extract from each group was subjected to Western blot with β-actin as the loading control. All the experiments were performed in triplicate.

    Article Snippet: The following antibodies were used: rabbit anti-FAK, rabbit anti-integrin αv and β3, mouse anti-β-actin, rabbit anti-pTyr100, peroxidase-conjugated goat anti-rabbit or anti-mouse IgG (all from Cell Signaling Technology, MA, USA) and rabbit anti-BKCa (Alomone Labs, Jerusalem, Israel).

    Techniques: Staining, Immunoprecipitation, Transfection, shRNA, Negative Control, Western Blot

    Modulation of FAK phosphorylation in PC3 cells by BKCa/αvβ3 complex A. Immunocolocalization of BKCa (red) and FAK (green). B. Co-immunoprecipitation of FAK and BKCa in PC3 cells. Cells were transfected with BKCa or BK-shRNA and their corresponding negative control vectors. Proteins were extracted and immunoprecipitated using anti-BKCa or anti-FAK antibodies; blots were probed with anti-FAK, anti-pTyr100 or anti-BKCa antibodies. Bead lanes contained the protein G conjugated sepharose beads used during the immunoprecipitation without the protein input. Equal amount of protein extract from each group was subjected to Western blot with β-actin as the loading control. C-D. Western blot analysis of the expression of BKCa, p-FAK (Y397), FAK and αvβ3 integrin in response to BKCa upregulation or downregulation. All the experiments were performed in triplicate. The data are shown as the means ± se. *P

    Journal: Oncotarget

    Article Title: BKCa promotes growth and metastasis of prostate cancer through facilitating the coupling between αvβ3 integrin and FAK

    doi: 10.18632/oncotarget.9559

    Figure Lengend Snippet: Modulation of FAK phosphorylation in PC3 cells by BKCa/αvβ3 complex A. Immunocolocalization of BKCa (red) and FAK (green). B. Co-immunoprecipitation of FAK and BKCa in PC3 cells. Cells were transfected with BKCa or BK-shRNA and their corresponding negative control vectors. Proteins were extracted and immunoprecipitated using anti-BKCa or anti-FAK antibodies; blots were probed with anti-FAK, anti-pTyr100 or anti-BKCa antibodies. Bead lanes contained the protein G conjugated sepharose beads used during the immunoprecipitation without the protein input. Equal amount of protein extract from each group was subjected to Western blot with β-actin as the loading control. C-D. Western blot analysis of the expression of BKCa, p-FAK (Y397), FAK and αvβ3 integrin in response to BKCa upregulation or downregulation. All the experiments were performed in triplicate. The data are shown as the means ± se. *P

    Article Snippet: The following antibodies were used: rabbit anti-FAK, rabbit anti-integrin αv and β3, mouse anti-β-actin, rabbit anti-pTyr100, peroxidase-conjugated goat anti-rabbit or anti-mouse IgG (all from Cell Signaling Technology, MA, USA) and rabbit anti-BKCa (Alomone Labs, Jerusalem, Israel).

    Techniques: Immunoprecipitation, Transfection, shRNA, Negative Control, Western Blot, Expressing

    Overexpression of BKCa stimulates prostate cancer cell proliferation, migration and invasion A. Prostate cancer PC3 and C4-2 cells were transfected with BKCa or Vector plasmids for 48 hr. The expression of BKCa was analyzed by western blot. β-actin was used as loading control. B. Cell growth curves as determined by MTT assay in PC3 and C4-2 cells transfected with BKCa or Vector. C. Percentage of EdU positive cells determined by EdU incorporation assay (lower panel) and representative images of EdU staining (upper panel) in PC3 and C4-2 cells transfected with BKCa or Vector. D. Effects of upregulated BKCa on the colony-genic ability of PC3 and C4-2 cells. Representative images were shown in the upper panel and the number of colonies was illustrated in the lower panel. E. Overexpression of BKCa promoted prostate cancer cell migration as determined by scratch wound healing assay. The representative images of wound healing were shown in left panel. The relative wound closure was illustrated in right panel. F-H. Effects of upregulated BKCa on the migration and invasion of PC3 and C4-2 cells as determined by transwell assays. Representative images and mean numbers of migrated (G) and invaded (H) cells were shown in the upper and lower panel respectively. All the experiments were performed in triplicate. The data are shown as the means ± se. *P

    Journal: Oncotarget

    Article Title: BKCa promotes growth and metastasis of prostate cancer through facilitating the coupling between αvβ3 integrin and FAK

    doi: 10.18632/oncotarget.9559

    Figure Lengend Snippet: Overexpression of BKCa stimulates prostate cancer cell proliferation, migration and invasion A. Prostate cancer PC3 and C4-2 cells were transfected with BKCa or Vector plasmids for 48 hr. The expression of BKCa was analyzed by western blot. β-actin was used as loading control. B. Cell growth curves as determined by MTT assay in PC3 and C4-2 cells transfected with BKCa or Vector. C. Percentage of EdU positive cells determined by EdU incorporation assay (lower panel) and representative images of EdU staining (upper panel) in PC3 and C4-2 cells transfected with BKCa or Vector. D. Effects of upregulated BKCa on the colony-genic ability of PC3 and C4-2 cells. Representative images were shown in the upper panel and the number of colonies was illustrated in the lower panel. E. Overexpression of BKCa promoted prostate cancer cell migration as determined by scratch wound healing assay. The representative images of wound healing were shown in left panel. The relative wound closure was illustrated in right panel. F-H. Effects of upregulated BKCa on the migration and invasion of PC3 and C4-2 cells as determined by transwell assays. Representative images and mean numbers of migrated (G) and invaded (H) cells were shown in the upper and lower panel respectively. All the experiments were performed in triplicate. The data are shown as the means ± se. *P

    Article Snippet: The following antibodies were used: rabbit anti-FAK, rabbit anti-integrin αv and β3, mouse anti-β-actin, rabbit anti-pTyr100, peroxidase-conjugated goat anti-rabbit or anti-mouse IgG (all from Cell Signaling Technology, MA, USA) and rabbit anti-BKCa (Alomone Labs, Jerusalem, Israel).

    Techniques: Over Expression, Migration, Transfection, Plasmid Preparation, Expressing, Western Blot, MTT Assay, Staining, Wound Healing Assay

    Expression analysis of BKCa in prostate cell lines A. Immunofluorescence staining of BKCa in cancerous prostate PC3, LNCaP and DU145 cells and normal prostate RWPE-1 cells. B. Real-time PCR analysis of BKCa mRNA in prostate cell lines. β-actin was used as internal control. The values were normalized to that of RWPE-1. C. Western blotting analysis of BKCa protein in prostate cell lines. β-actin was used as internal control and the values were normalized to that of RWPE-1. *P

    Journal: Oncotarget

    Article Title: BKCa promotes growth and metastasis of prostate cancer through facilitating the coupling between αvβ3 integrin and FAK

    doi: 10.18632/oncotarget.9559

    Figure Lengend Snippet: Expression analysis of BKCa in prostate cell lines A. Immunofluorescence staining of BKCa in cancerous prostate PC3, LNCaP and DU145 cells and normal prostate RWPE-1 cells. B. Real-time PCR analysis of BKCa mRNA in prostate cell lines. β-actin was used as internal control. The values were normalized to that of RWPE-1. C. Western blotting analysis of BKCa protein in prostate cell lines. β-actin was used as internal control and the values were normalized to that of RWPE-1. *P

    Article Snippet: The following antibodies were used: rabbit anti-FAK, rabbit anti-integrin αv and β3, mouse anti-β-actin, rabbit anti-pTyr100, peroxidase-conjugated goat anti-rabbit or anti-mouse IgG (all from Cell Signaling Technology, MA, USA) and rabbit anti-BKCa (Alomone Labs, Jerusalem, Israel).

    Techniques: Expressing, Immunofluorescence, Staining, Real-time Polymerase Chain Reaction, Western Blot

    Effects of berberine on glucose consumption and lactate release upon AMPKα1/α2 silencing in HepG2 hepatocytes. A: HepG2 hepatocytes were seeded in 24-well plates, and transfected with various doses of scrambled siRNA and/or AMPKα1/α2 siRNA for 24 h. The protein level was examined by Western blot. Ratio of AMPKα to β-actin was quantified in 3 independent experiments per condition. It had been found that AMPKα1/α2 siRNA at 30 pmol/well most effectively suppressed the expression of AMPKα. * P

    Journal: PLoS ONE

    Article Title: Berberine Promotes Glucose Consumption Independently of AMP-Activated Protein Kinase Activation

    doi: 10.1371/journal.pone.0103702

    Figure Lengend Snippet: Effects of berberine on glucose consumption and lactate release upon AMPKα1/α2 silencing in HepG2 hepatocytes. A: HepG2 hepatocytes were seeded in 24-well plates, and transfected with various doses of scrambled siRNA and/or AMPKα1/α2 siRNA for 24 h. The protein level was examined by Western blot. Ratio of AMPKα to β-actin was quantified in 3 independent experiments per condition. It had been found that AMPKα1/α2 siRNA at 30 pmol/well most effectively suppressed the expression of AMPKα. * P

    Article Snippet: Antibodies to AMPK, acetyl coenzyme A synthetase (ACC), phospho-AMPK (Thr172), β-actin and phospho-ACC (Ser-79) were purchased from Cell Signaling Technology (Danvers, MA).

    Techniques: Transfection, Western Blot, Expressing