Structured Review

Yeasen Biotechnology annexin v fitc pi apoptosis detection kit
( A ) Immunoblotting analyses of FADD protein levels in MDA-MB-231 and SUM159PT cells stably expressing shNC or shSF3A2 (#1 and #2). ( B ) Flow cytometry analysis of cell <t>apoptosis</t> was conducted using MDA-MB-231 and SUM159PT cells stably expressing shNC or shSF3A2 (#1 and #2). ( C ) In vivo ubiquitination assay demonstrating the ubiquitinated FADD proteins. HEK293T cells transfected with the indicated plasmid were treated with 10 μM MG132 for 6 hours, followed by IP and subsequent immunoblotting analyses. ( D ) Immunoblotting analyses of γH2AX and cleaved caspase-3 protein levels in MDA-MB-231 and SUM159PT cells stably expressing shNC or shSF3A2 (#1 and #2). ( E and F ) MDA-MB-231 and SUM159PT cells stably expressing shNC or shSF3A2 (#1 and #2) were subjected to immunofluorescence staining for γH2AX or 4′,6-diamidino-2-phenylindole (DAPI). Representative images are shown in [(E), scale bars, 10 μm] and corresponding calculations of clustered foci are displayed in (F). ( G ) SF3A2 knockdown endows sensitivity to cisplatin in TNBC cells as detected by CCK8 assay. ( H ) SF3A2 ablation confers sensitivity to cisplatin in TNBC cells as confirmed by colony formation assay. Quantitative results of survival colonies are shown in (H) and corresponding representative images are provided in fig. S9C. ( I to K ) A total of 5 × 10 6 MDA-MB-231 cells stably expressing shNC or shSF3A2 #2 were transplanted into the mammary fat pads of mice ( n = 10). Cisplatin treatment (3 mg/kg in 1× PBS) was initiated when tumors reached 100 mm 3 and administered twice a week via intraperitoneal injection. Tumor growth curve (I), tumor photograph (J), and weight (K) are shown. ( L ) The proposed working model.
Annexin V Fitc Pi Apoptosis Detection Kit, supplied by Yeasen Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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annexin v fitc pi apoptosis detection kit - by Bioz Stars, 2024-04
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1) Product Images from "SF3A2 promotes progression and cisplatin resistance in triple-negative breast cancer via alternative splicing of MKRN1"

Article Title: SF3A2 promotes progression and cisplatin resistance in triple-negative breast cancer via alternative splicing of MKRN1

Journal: Science Advances

doi: 10.1126/sciadv.adj4009

( A ) Immunoblotting analyses of FADD protein levels in MDA-MB-231 and SUM159PT cells stably expressing shNC or shSF3A2 (#1 and #2). ( B ) Flow cytometry analysis of cell apoptosis was conducted using MDA-MB-231 and SUM159PT cells stably expressing shNC or shSF3A2 (#1 and #2). ( C ) In vivo ubiquitination assay demonstrating the ubiquitinated FADD proteins. HEK293T cells transfected with the indicated plasmid were treated with 10 μM MG132 for 6 hours, followed by IP and subsequent immunoblotting analyses. ( D ) Immunoblotting analyses of γH2AX and cleaved caspase-3 protein levels in MDA-MB-231 and SUM159PT cells stably expressing shNC or shSF3A2 (#1 and #2). ( E and F ) MDA-MB-231 and SUM159PT cells stably expressing shNC or shSF3A2 (#1 and #2) were subjected to immunofluorescence staining for γH2AX or 4′,6-diamidino-2-phenylindole (DAPI). Representative images are shown in [(E), scale bars, 10 μm] and corresponding calculations of clustered foci are displayed in (F). ( G ) SF3A2 knockdown endows sensitivity to cisplatin in TNBC cells as detected by CCK8 assay. ( H ) SF3A2 ablation confers sensitivity to cisplatin in TNBC cells as confirmed by colony formation assay. Quantitative results of survival colonies are shown in (H) and corresponding representative images are provided in fig. S9C. ( I to K ) A total of 5 × 10 6 MDA-MB-231 cells stably expressing shNC or shSF3A2 #2 were transplanted into the mammary fat pads of mice ( n = 10). Cisplatin treatment (3 mg/kg in 1× PBS) was initiated when tumors reached 100 mm 3 and administered twice a week via intraperitoneal injection. Tumor growth curve (I), tumor photograph (J), and weight (K) are shown. ( L ) The proposed working model.
Figure Legend Snippet: ( A ) Immunoblotting analyses of FADD protein levels in MDA-MB-231 and SUM159PT cells stably expressing shNC or shSF3A2 (#1 and #2). ( B ) Flow cytometry analysis of cell apoptosis was conducted using MDA-MB-231 and SUM159PT cells stably expressing shNC or shSF3A2 (#1 and #2). ( C ) In vivo ubiquitination assay demonstrating the ubiquitinated FADD proteins. HEK293T cells transfected with the indicated plasmid were treated with 10 μM MG132 for 6 hours, followed by IP and subsequent immunoblotting analyses. ( D ) Immunoblotting analyses of γH2AX and cleaved caspase-3 protein levels in MDA-MB-231 and SUM159PT cells stably expressing shNC or shSF3A2 (#1 and #2). ( E and F ) MDA-MB-231 and SUM159PT cells stably expressing shNC or shSF3A2 (#1 and #2) were subjected to immunofluorescence staining for γH2AX or 4′,6-diamidino-2-phenylindole (DAPI). Representative images are shown in [(E), scale bars, 10 μm] and corresponding calculations of clustered foci are displayed in (F). ( G ) SF3A2 knockdown endows sensitivity to cisplatin in TNBC cells as detected by CCK8 assay. ( H ) SF3A2 ablation confers sensitivity to cisplatin in TNBC cells as confirmed by colony formation assay. Quantitative results of survival colonies are shown in (H) and corresponding representative images are provided in fig. S9C. ( I to K ) A total of 5 × 10 6 MDA-MB-231 cells stably expressing shNC or shSF3A2 #2 were transplanted into the mammary fat pads of mice ( n = 10). Cisplatin treatment (3 mg/kg in 1× PBS) was initiated when tumors reached 100 mm 3 and administered twice a week via intraperitoneal injection. Tumor growth curve (I), tumor photograph (J), and weight (K) are shown. ( L ) The proposed working model.

Techniques Used: Western Blot, Stable Transfection, Expressing, Flow Cytometry, In Vivo, Ubiquitin Assay, Transfection, Plasmid Preparation, Immunofluorescence, Staining, CCK-8 Assay, Colony Assay, Injection


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Yeasen Biotechnology annexin v fitc pi apoptosis detection kit
Annexin V Fitc Pi Apoptosis Detection Kit, supplied by Yeasen Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/annexin v fitc pi apoptosis detection kit/product/Yeasen Biotechnology
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
annexin v fitc pi apoptosis detection kit - by Bioz Stars, 2024-04
86/100 stars

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Structured Review

Yeasen Biotechnology annexin v fitc pi apoptosis detection kit
Annexin V Fitc Pi Apoptosis Detection Kit, supplied by Yeasen Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/annexin v fitc pi apoptosis detection kit/product/Yeasen Biotechnology
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
annexin v fitc pi apoptosis detection kit - by Bioz Stars, 2024-04
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Yeasen Biotechnology annexin v fitc pi apoptosis detection kit
SLC2A1 and ACSL4 were overexpressed in AKI and promote HK-2 cell <t>apoptosis.</t> ( A ) HE and immunohistochemistry of SLC2A1, GPX4 and TEM in renal ischemia-reperfusion injury model; ( B ) The protein level of SLC2A1 and ACSL4 in renal ischemia-reperfusion injury model and normal control model was verified by Western blot; ( C ) Relative quantification of SLC2A1 and ASCL4 in B; ( D ) The protein level of ACSL4 and SLC2A1 in HR induced AKI model and was verified by Western blot; ( E ) Relative quantification of these proteins in D; ( F ) The lipid ROS level was verified in HR induced AKI model and normal control model; ( G ) The MDA level was verified in HR induced AKI model and normal control model; ( H , I ) The apoptosis rate was verified in HR induced AKI model and normal control model. * p < 0.05, ** p < 0.01, *** p < 0.001. Abbreviations: MDA: Malondialdehyde; ROS: Reactive Oxygen Species.
Annexin V Fitc Pi Apoptosis Detection Kit, supplied by Yeasen Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/annexin v fitc pi apoptosis detection kit/product/Yeasen Biotechnology
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
annexin v fitc pi apoptosis detection kit - by Bioz Stars, 2024-04
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1) Product Images from "Identification and validation of ferroptosis-related gene SLC2A1 as a novel prognostic biomarker in AKI"

Article Title: Identification and validation of ferroptosis-related gene SLC2A1 as a novel prognostic biomarker in AKI

Journal: Aging (Albany NY)

doi: 10.18632/aging.205669

SLC2A1 and ACSL4 were overexpressed in AKI and promote HK-2 cell apoptosis. ( A ) HE and immunohistochemistry of SLC2A1, GPX4 and TEM in renal ischemia-reperfusion injury model; ( B ) The protein level of SLC2A1 and ACSL4 in renal ischemia-reperfusion injury model and normal control model was verified by Western blot; ( C ) Relative quantification of SLC2A1 and ASCL4 in B; ( D ) The protein level of ACSL4 and SLC2A1 in HR induced AKI model and was verified by Western blot; ( E ) Relative quantification of these proteins in D; ( F ) The lipid ROS level was verified in HR induced AKI model and normal control model; ( G ) The MDA level was verified in HR induced AKI model and normal control model; ( H , I ) The apoptosis rate was verified in HR induced AKI model and normal control model. * p < 0.05, ** p < 0.01, *** p < 0.001. Abbreviations: MDA: Malondialdehyde; ROS: Reactive Oxygen Species.
Figure Legend Snippet: SLC2A1 and ACSL4 were overexpressed in AKI and promote HK-2 cell apoptosis. ( A ) HE and immunohistochemistry of SLC2A1, GPX4 and TEM in renal ischemia-reperfusion injury model; ( B ) The protein level of SLC2A1 and ACSL4 in renal ischemia-reperfusion injury model and normal control model was verified by Western blot; ( C ) Relative quantification of SLC2A1 and ASCL4 in B; ( D ) The protein level of ACSL4 and SLC2A1 in HR induced AKI model and was verified by Western blot; ( E ) Relative quantification of these proteins in D; ( F ) The lipid ROS level was verified in HR induced AKI model and normal control model; ( G ) The MDA level was verified in HR induced AKI model and normal control model; ( H , I ) The apoptosis rate was verified in HR induced AKI model and normal control model. * p < 0.05, ** p < 0.01, *** p < 0.001. Abbreviations: MDA: Malondialdehyde; ROS: Reactive Oxygen Species.

Techniques Used: Immunohistochemistry, Western Blot

SLC2A1 promotes HK-2 cell apoptosis and ferroptosis via ACSL4. ( A , B ) The protein level of ACSL4 and SLC2A1 in HR induced AKI mode when overexpressed SLC2A1, overexpressed SLC2A1+T, or T was verified by Western blot and relative quantification of these proteins; ( C ) The lipid ROS level was verified in HR induced AKI mode when overexpressed SLC2A1, overexpressed SLC2A1+T, or T; ( D ) The MDA level was verified in HR induced AKI model when overexpressed SLC2A1, overexpressed SLC2A1+T, or T; ( E – G ) The apoptosis rate was verified in HR induced AKI model when overexpressed SLC2A1, overexpressed SLC2A1+T, or T. * p < 0.05, ** p < 0.01, *** p < 0.001. Abbreviations: T: Troglitazone; MDA: Malondialdehyde; ROS: Reactive Oxygen Species.
Figure Legend Snippet: SLC2A1 promotes HK-2 cell apoptosis and ferroptosis via ACSL4. ( A , B ) The protein level of ACSL4 and SLC2A1 in HR induced AKI mode when overexpressed SLC2A1, overexpressed SLC2A1+T, or T was verified by Western blot and relative quantification of these proteins; ( C ) The lipid ROS level was verified in HR induced AKI mode when overexpressed SLC2A1, overexpressed SLC2A1+T, or T; ( D ) The MDA level was verified in HR induced AKI model when overexpressed SLC2A1, overexpressed SLC2A1+T, or T; ( E – G ) The apoptosis rate was verified in HR induced AKI model when overexpressed SLC2A1, overexpressed SLC2A1+T, or T. * p < 0.05, ** p < 0.01, *** p < 0.001. Abbreviations: T: Troglitazone; MDA: Malondialdehyde; ROS: Reactive Oxygen Species.

Techniques Used: Western Blot


Structured Review

Yeasen Biotechnology annexin v fitc pi apoptosis detection kit
a Evaluation of photosensitizer (PS) binding in 4T1 cells after 1 h treatment with P-PS or P-BS-PS, or sequential treatment with P-BS-CM1 for 1 h followed by P-PS-CM2 for 1 h by flow cytometry analysis. b Representative confocal microscopy images of 4T1 cells treated with P-PS or P-BS-PS for 1 h, or consecutively treated with P-BS-CM1 for 1 h followed by P-PS-CM2 for 1 h. Blue indicates cell nuclei, red indicates PS fluorophores. Scale bars, 20 μm. c – f Evaluations in 4T1 cells after various treatments with laser irradiation (+), including: ( c ) Cell viability; ( d ), <t>Apoptosis</t> induction; ( e ), Intracellular expression of phosphatidylinositol 3-kinase (PI3K); ( f ), ICD hallmarks of calreticulin (CRT) exposure and extracellular adenosine triphosphate (ATP) release. For P-PS and P-BS-PS groups, treatments were administered for 1 h followed by 5 min of laser irradiation and 24 h of further culture prior to analysis. For the P-BS-CM1 → P-PS-CM2 group, consecutive 1 h treatments with P-BS-CM1 and P-PS-CM2 were administered, followed by 5 min of laser irradiation and 24 h of culture prior to analysis. n = 3 biologically independent samples per group ( c , f ). All the experiments were repeated twice independently with similar results. Data are presented as mean ± SD. Statistics are calculated by one-way ANOVA with Tukey’s multiple comparisons without adjustments. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001. Source data are provided as a Source Data file.
Annexin V Fitc Pi Apoptosis Detection Kit, supplied by Yeasen Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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annexin v fitc pi apoptosis detection kit - by Bioz Stars, 2024-04
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1) Product Images from "Cell surface patching via CXCR4-targeted nanothreads for cancer metastasis inhibition"

Article Title: Cell surface patching via CXCR4-targeted nanothreads for cancer metastasis inhibition

Journal: Nature Communications

doi: 10.1038/s41467-024-47111-z

a Evaluation of photosensitizer (PS) binding in 4T1 cells after 1 h treatment with P-PS or P-BS-PS, or sequential treatment with P-BS-CM1 for 1 h followed by P-PS-CM2 for 1 h by flow cytometry analysis. b Representative confocal microscopy images of 4T1 cells treated with P-PS or P-BS-PS for 1 h, or consecutively treated with P-BS-CM1 for 1 h followed by P-PS-CM2 for 1 h. Blue indicates cell nuclei, red indicates PS fluorophores. Scale bars, 20 μm. c – f Evaluations in 4T1 cells after various treatments with laser irradiation (+), including: ( c ) Cell viability; ( d ), Apoptosis induction; ( e ), Intracellular expression of phosphatidylinositol 3-kinase (PI3K); ( f ), ICD hallmarks of calreticulin (CRT) exposure and extracellular adenosine triphosphate (ATP) release. For P-PS and P-BS-PS groups, treatments were administered for 1 h followed by 5 min of laser irradiation and 24 h of further culture prior to analysis. For the P-BS-CM1 → P-PS-CM2 group, consecutive 1 h treatments with P-BS-CM1 and P-PS-CM2 were administered, followed by 5 min of laser irradiation and 24 h of culture prior to analysis. n = 3 biologically independent samples per group ( c , f ). All the experiments were repeated twice independently with similar results. Data are presented as mean ± SD. Statistics are calculated by one-way ANOVA with Tukey’s multiple comparisons without adjustments. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001. Source data are provided as a Source Data file.
Figure Legend Snippet: a Evaluation of photosensitizer (PS) binding in 4T1 cells after 1 h treatment with P-PS or P-BS-PS, or sequential treatment with P-BS-CM1 for 1 h followed by P-PS-CM2 for 1 h by flow cytometry analysis. b Representative confocal microscopy images of 4T1 cells treated with P-PS or P-BS-PS for 1 h, or consecutively treated with P-BS-CM1 for 1 h followed by P-PS-CM2 for 1 h. Blue indicates cell nuclei, red indicates PS fluorophores. Scale bars, 20 μm. c – f Evaluations in 4T1 cells after various treatments with laser irradiation (+), including: ( c ) Cell viability; ( d ), Apoptosis induction; ( e ), Intracellular expression of phosphatidylinositol 3-kinase (PI3K); ( f ), ICD hallmarks of calreticulin (CRT) exposure and extracellular adenosine triphosphate (ATP) release. For P-PS and P-BS-PS groups, treatments were administered for 1 h followed by 5 min of laser irradiation and 24 h of further culture prior to analysis. For the P-BS-CM1 → P-PS-CM2 group, consecutive 1 h treatments with P-BS-CM1 and P-PS-CM2 were administered, followed by 5 min of laser irradiation and 24 h of culture prior to analysis. n = 3 biologically independent samples per group ( c , f ). All the experiments were repeated twice independently with similar results. Data are presented as mean ± SD. Statistics are calculated by one-way ANOVA with Tukey’s multiple comparisons without adjustments. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001. Source data are provided as a Source Data file.

Techniques Used: Binding Assay, Flow Cytometry, Confocal Microscopy, Irradiation, Expressing


Structured Review

Yeasen Biotechnology annexin v fitc pi apoptosis detection kit
a Cytotoxicity and IC 50 of various formulations as determined by the MTT assay in H1975, A549, and A549/DDP cells after 24 h incubation ( n = 3 independent samples; one-way ANOVA followed by Tukey’s HSD post hoc test). FI is defined as IC 50 (Cis)/IC 50 (FP NPs) b Inverted microscopy images of H1975, A549, and A549/DDP cells calcein AM/PI double staining after 6 h treatment with 4 μM different formulations (dosage based on Fluplatin; n = 3 independent samples). Scale bars, 100 μm. c The <t>Apoptosis</t> rate of H1975 cells after incubation with 4 μM different formulations (dosage based on Fluplatin) for 6 h was determined by flow cytometry (FACS) analysis. d The fold rate of caspas3/9 enzymatic activities was detected after 6 h treatment with 4 μM different formulations (dosage based on Fluplatin; n = 3 independent samples; one-way ANOVA followed by Tukey’s HSD post hoc test). After Pifithrin-α and STI571 treatment for 24 h, the cytotoxicity and IC 50 of cisplatin treatment in H1299 cells transfected with expression constructs containing wtp53 ( e ), with vector ( f ), and expression constructs containing the R273H variant ( g ) were measured by the MTT method, n = 3 independent samples; one-way ANOVA followed by Tukey’s HSD post hoc test for ( e , f , g) . STI571 (STI), pifithrin-α (pif). Data are shown as the mean ± SD; n.s. no significance. Source data are provided as a file.
Annexin V Fitc Pi Apoptosis Detection Kit, supplied by Yeasen Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/annexin v fitc pi apoptosis detection kit/product/Yeasen Biotechnology
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
annexin v fitc pi apoptosis detection kit - by Bioz Stars, 2024-04
86/100 stars

Images

1) Product Images from "Nanoparticles targeting mutant p53 overcome chemoresistance and tumor recurrence in non-small cell lung cancer"

Article Title: Nanoparticles targeting mutant p53 overcome chemoresistance and tumor recurrence in non-small cell lung cancer

Journal: Nature Communications

doi: 10.1038/s41467-024-47080-3

a Cytotoxicity and IC 50 of various formulations as determined by the MTT assay in H1975, A549, and A549/DDP cells after 24 h incubation ( n = 3 independent samples; one-way ANOVA followed by Tukey’s HSD post hoc test). FI is defined as IC 50 (Cis)/IC 50 (FP NPs) b Inverted microscopy images of H1975, A549, and A549/DDP cells calcein AM/PI double staining after 6 h treatment with 4 μM different formulations (dosage based on Fluplatin; n = 3 independent samples). Scale bars, 100 μm. c The Apoptosis rate of H1975 cells after incubation with 4 μM different formulations (dosage based on Fluplatin) for 6 h was determined by flow cytometry (FACS) analysis. d The fold rate of caspas3/9 enzymatic activities was detected after 6 h treatment with 4 μM different formulations (dosage based on Fluplatin; n = 3 independent samples; one-way ANOVA followed by Tukey’s HSD post hoc test). After Pifithrin-α and STI571 treatment for 24 h, the cytotoxicity and IC 50 of cisplatin treatment in H1299 cells transfected with expression constructs containing wtp53 ( e ), with vector ( f ), and expression constructs containing the R273H variant ( g ) were measured by the MTT method, n = 3 independent samples; one-way ANOVA followed by Tukey’s HSD post hoc test for ( e , f , g) . STI571 (STI), pifithrin-α (pif). Data are shown as the mean ± SD; n.s. no significance. Source data are provided as a file.
Figure Legend Snippet: a Cytotoxicity and IC 50 of various formulations as determined by the MTT assay in H1975, A549, and A549/DDP cells after 24 h incubation ( n = 3 independent samples; one-way ANOVA followed by Tukey’s HSD post hoc test). FI is defined as IC 50 (Cis)/IC 50 (FP NPs) b Inverted microscopy images of H1975, A549, and A549/DDP cells calcein AM/PI double staining after 6 h treatment with 4 μM different formulations (dosage based on Fluplatin; n = 3 independent samples). Scale bars, 100 μm. c The Apoptosis rate of H1975 cells after incubation with 4 μM different formulations (dosage based on Fluplatin) for 6 h was determined by flow cytometry (FACS) analysis. d The fold rate of caspas3/9 enzymatic activities was detected after 6 h treatment with 4 μM different formulations (dosage based on Fluplatin; n = 3 independent samples; one-way ANOVA followed by Tukey’s HSD post hoc test). After Pifithrin-α and STI571 treatment for 24 h, the cytotoxicity and IC 50 of cisplatin treatment in H1299 cells transfected with expression constructs containing wtp53 ( e ), with vector ( f ), and expression constructs containing the R273H variant ( g ) were measured by the MTT method, n = 3 independent samples; one-way ANOVA followed by Tukey’s HSD post hoc test for ( e , f , g) . STI571 (STI), pifithrin-α (pif). Data are shown as the mean ± SD; n.s. no significance. Source data are provided as a file.

Techniques Used: MTT Assay, Incubation, Inverted Microscopy, Double Staining, Flow Cytometry, Transfection, Expressing, Construct, Plasmid Preparation, Variant Assay


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Yeasen Biotechnology annexin v fitc pi cell apoptosis kit
Annexin V Fitc Pi Cell Apoptosis Kit, supplied by Yeasen Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/annexin v fitc pi cell apoptosis kit/product/Yeasen Biotechnology
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annexin v fitc pi cell apoptosis kit - by Bioz Stars, 2024-04
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Yeasen Biotechnology annexin v fitc pi apoptosis detection kit
PDZK1-mediated modulation of cell <t>apoptosis</t> and cell cycle arrest in HCC cells. Cell apoptosis assay results for different treatments in MHCC-97H cells ( A ) and Huh7 cells ( B ). Cell cycle assay results for different treatments in MHCC-97H cells ( C ) and Huh7 cells ( D ). Data are expressed as the mean ± SD values (n = 3). * P < 0.05, ** P < 0.01, *** P < 0.001
Annexin V Fitc Pi Apoptosis Detection Kit, supplied by Yeasen Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/annexin v fitc pi apoptosis detection kit/product/Yeasen Biotechnology
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
annexin v fitc pi apoptosis detection kit - by Bioz Stars, 2024-04
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1) Product Images from "miR-101-3p-mediated role of PDZK1 in hepatocellular carcinoma progression and the underlying PI3K/Akt signaling mechanism"

Article Title: miR-101-3p-mediated role of PDZK1 in hepatocellular carcinoma progression and the underlying PI3K/Akt signaling mechanism

Journal: Cell Division

doi: 10.1186/s13008-023-00106-6

PDZK1-mediated modulation of cell apoptosis and cell cycle arrest in HCC cells. Cell apoptosis assay results for different treatments in MHCC-97H cells ( A ) and Huh7 cells ( B ). Cell cycle assay results for different treatments in MHCC-97H cells ( C ) and Huh7 cells ( D ). Data are expressed as the mean ± SD values (n = 3). * P < 0.05, ** P < 0.01, *** P < 0.001
Figure Legend Snippet: PDZK1-mediated modulation of cell apoptosis and cell cycle arrest in HCC cells. Cell apoptosis assay results for different treatments in MHCC-97H cells ( A ) and Huh7 cells ( B ). Cell cycle assay results for different treatments in MHCC-97H cells ( C ) and Huh7 cells ( D ). Data are expressed as the mean ± SD values (n = 3). * P < 0.05, ** P < 0.01, *** P < 0.001

Techniques Used: Apoptosis Assay, Cell Cycle Assay


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Yeasen Biotechnology annexin v fitc pi apoptosis detection kit
Annexin V Fitc Pi Apoptosis Detection Kit, supplied by Yeasen Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/annexin v fitc pi apoptosis detection kit/product/Yeasen Biotechnology
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annexin v fitc propyl iodide pi apoptosis detection kit  (Yeasen Biotechnology)

 
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    Yeasen Biotechnology annexin v fitc propyl iodide pi apoptosis detection kit
    Annexin V Fitc Propyl Iodide Pi Apoptosis Detection Kit, supplied by Yeasen Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Yeasen Biotechnology annexin v fitc pi apoptosis detection kit
    ( A ) Immunoblotting analyses of FADD protein levels in MDA-MB-231 and SUM159PT cells stably expressing shNC or shSF3A2 (#1 and #2). ( B ) Flow cytometry analysis of cell <t>apoptosis</t> was conducted using MDA-MB-231 and SUM159PT cells stably expressing shNC or shSF3A2 (#1 and #2). ( C ) In vivo ubiquitination assay demonstrating the ubiquitinated FADD proteins. HEK293T cells transfected with the indicated plasmid were treated with 10 μM MG132 for 6 hours, followed by IP and subsequent immunoblotting analyses. ( D ) Immunoblotting analyses of γH2AX and cleaved caspase-3 protein levels in MDA-MB-231 and SUM159PT cells stably expressing shNC or shSF3A2 (#1 and #2). ( E and F ) MDA-MB-231 and SUM159PT cells stably expressing shNC or shSF3A2 (#1 and #2) were subjected to immunofluorescence staining for γH2AX or 4′,6-diamidino-2-phenylindole (DAPI). Representative images are shown in [(E), scale bars, 10 μm] and corresponding calculations of clustered foci are displayed in (F). ( G ) SF3A2 knockdown endows sensitivity to cisplatin in TNBC cells as detected by CCK8 assay. ( H ) SF3A2 ablation confers sensitivity to cisplatin in TNBC cells as confirmed by colony formation assay. Quantitative results of survival colonies are shown in (H) and corresponding representative images are provided in fig. S9C. ( I to K ) A total of 5 × 10 6 MDA-MB-231 cells stably expressing shNC or shSF3A2 #2 were transplanted into the mammary fat pads of mice ( n = 10). Cisplatin treatment (3 mg/kg in 1× PBS) was initiated when tumors reached 100 mm 3 and administered twice a week via intraperitoneal injection. Tumor growth curve (I), tumor photograph (J), and weight (K) are shown. ( L ) The proposed working model.
    Annexin V Fitc Pi Apoptosis Detection Kit, supplied by Yeasen Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/annexin v fitc pi apoptosis detection kit/product/Yeasen Biotechnology
    Average 86 stars, based on 1 article reviews
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    annexin v fitc pi apoptosis detection kit - by Bioz Stars, 2024-04
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    86
    Yeasen Biotechnology annexin v fitc pi cell apoptosis kit
    ( A ) Immunoblotting analyses of FADD protein levels in MDA-MB-231 and SUM159PT cells stably expressing shNC or shSF3A2 (#1 and #2). ( B ) Flow cytometry analysis of cell <t>apoptosis</t> was conducted using MDA-MB-231 and SUM159PT cells stably expressing shNC or shSF3A2 (#1 and #2). ( C ) In vivo ubiquitination assay demonstrating the ubiquitinated FADD proteins. HEK293T cells transfected with the indicated plasmid were treated with 10 μM MG132 for 6 hours, followed by IP and subsequent immunoblotting analyses. ( D ) Immunoblotting analyses of γH2AX and cleaved caspase-3 protein levels in MDA-MB-231 and SUM159PT cells stably expressing shNC or shSF3A2 (#1 and #2). ( E and F ) MDA-MB-231 and SUM159PT cells stably expressing shNC or shSF3A2 (#1 and #2) were subjected to immunofluorescence staining for γH2AX or 4′,6-diamidino-2-phenylindole (DAPI). Representative images are shown in [(E), scale bars, 10 μm] and corresponding calculations of clustered foci are displayed in (F). ( G ) SF3A2 knockdown endows sensitivity to cisplatin in TNBC cells as detected by CCK8 assay. ( H ) SF3A2 ablation confers sensitivity to cisplatin in TNBC cells as confirmed by colony formation assay. Quantitative results of survival colonies are shown in (H) and corresponding representative images are provided in fig. S9C. ( I to K ) A total of 5 × 10 6 MDA-MB-231 cells stably expressing shNC or shSF3A2 #2 were transplanted into the mammary fat pads of mice ( n = 10). Cisplatin treatment (3 mg/kg in 1× PBS) was initiated when tumors reached 100 mm 3 and administered twice a week via intraperitoneal injection. Tumor growth curve (I), tumor photograph (J), and weight (K) are shown. ( L ) The proposed working model.
    Annexin V Fitc Pi Cell Apoptosis Kit, supplied by Yeasen Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Yeasen Biotechnology annexin v fitc propyl iodide pi apoptosis detection kit
    ( A ) Immunoblotting analyses of FADD protein levels in MDA-MB-231 and SUM159PT cells stably expressing shNC or shSF3A2 (#1 and #2). ( B ) Flow cytometry analysis of cell <t>apoptosis</t> was conducted using MDA-MB-231 and SUM159PT cells stably expressing shNC or shSF3A2 (#1 and #2). ( C ) In vivo ubiquitination assay demonstrating the ubiquitinated FADD proteins. HEK293T cells transfected with the indicated plasmid were treated with 10 μM MG132 for 6 hours, followed by IP and subsequent immunoblotting analyses. ( D ) Immunoblotting analyses of γH2AX and cleaved caspase-3 protein levels in MDA-MB-231 and SUM159PT cells stably expressing shNC or shSF3A2 (#1 and #2). ( E and F ) MDA-MB-231 and SUM159PT cells stably expressing shNC or shSF3A2 (#1 and #2) were subjected to immunofluorescence staining for γH2AX or 4′,6-diamidino-2-phenylindole (DAPI). Representative images are shown in [(E), scale bars, 10 μm] and corresponding calculations of clustered foci are displayed in (F). ( G ) SF3A2 knockdown endows sensitivity to cisplatin in TNBC cells as detected by CCK8 assay. ( H ) SF3A2 ablation confers sensitivity to cisplatin in TNBC cells as confirmed by colony formation assay. Quantitative results of survival colonies are shown in (H) and corresponding representative images are provided in fig. S9C. ( I to K ) A total of 5 × 10 6 MDA-MB-231 cells stably expressing shNC or shSF3A2 #2 were transplanted into the mammary fat pads of mice ( n = 10). Cisplatin treatment (3 mg/kg in 1× PBS) was initiated when tumors reached 100 mm 3 and administered twice a week via intraperitoneal injection. Tumor growth curve (I), tumor photograph (J), and weight (K) are shown. ( L ) The proposed working model.
    Annexin V Fitc Propyl Iodide Pi Apoptosis Detection Kit, supplied by Yeasen Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    ( A ) Immunoblotting analyses of FADD protein levels in MDA-MB-231 and SUM159PT cells stably expressing shNC or shSF3A2 (#1 and #2). ( B ) Flow cytometry analysis of cell apoptosis was conducted using MDA-MB-231 and SUM159PT cells stably expressing shNC or shSF3A2 (#1 and #2). ( C ) In vivo ubiquitination assay demonstrating the ubiquitinated FADD proteins. HEK293T cells transfected with the indicated plasmid were treated with 10 μM MG132 for 6 hours, followed by IP and subsequent immunoblotting analyses. ( D ) Immunoblotting analyses of γH2AX and cleaved caspase-3 protein levels in MDA-MB-231 and SUM159PT cells stably expressing shNC or shSF3A2 (#1 and #2). ( E and F ) MDA-MB-231 and SUM159PT cells stably expressing shNC or shSF3A2 (#1 and #2) were subjected to immunofluorescence staining for γH2AX or 4′,6-diamidino-2-phenylindole (DAPI). Representative images are shown in [(E), scale bars, 10 μm] and corresponding calculations of clustered foci are displayed in (F). ( G ) SF3A2 knockdown endows sensitivity to cisplatin in TNBC cells as detected by CCK8 assay. ( H ) SF3A2 ablation confers sensitivity to cisplatin in TNBC cells as confirmed by colony formation assay. Quantitative results of survival colonies are shown in (H) and corresponding representative images are provided in fig. S9C. ( I to K ) A total of 5 × 10 6 MDA-MB-231 cells stably expressing shNC or shSF3A2 #2 were transplanted into the mammary fat pads of mice ( n = 10). Cisplatin treatment (3 mg/kg in 1× PBS) was initiated when tumors reached 100 mm 3 and administered twice a week via intraperitoneal injection. Tumor growth curve (I), tumor photograph (J), and weight (K) are shown. ( L ) The proposed working model.

    Journal: Science Advances

    Article Title: SF3A2 promotes progression and cisplatin resistance in triple-negative breast cancer via alternative splicing of MKRN1

    doi: 10.1126/sciadv.adj4009

    Figure Lengend Snippet: ( A ) Immunoblotting analyses of FADD protein levels in MDA-MB-231 and SUM159PT cells stably expressing shNC or shSF3A2 (#1 and #2). ( B ) Flow cytometry analysis of cell apoptosis was conducted using MDA-MB-231 and SUM159PT cells stably expressing shNC or shSF3A2 (#1 and #2). ( C ) In vivo ubiquitination assay demonstrating the ubiquitinated FADD proteins. HEK293T cells transfected with the indicated plasmid were treated with 10 μM MG132 for 6 hours, followed by IP and subsequent immunoblotting analyses. ( D ) Immunoblotting analyses of γH2AX and cleaved caspase-3 protein levels in MDA-MB-231 and SUM159PT cells stably expressing shNC or shSF3A2 (#1 and #2). ( E and F ) MDA-MB-231 and SUM159PT cells stably expressing shNC or shSF3A2 (#1 and #2) were subjected to immunofluorescence staining for γH2AX or 4′,6-diamidino-2-phenylindole (DAPI). Representative images are shown in [(E), scale bars, 10 μm] and corresponding calculations of clustered foci are displayed in (F). ( G ) SF3A2 knockdown endows sensitivity to cisplatin in TNBC cells as detected by CCK8 assay. ( H ) SF3A2 ablation confers sensitivity to cisplatin in TNBC cells as confirmed by colony formation assay. Quantitative results of survival colonies are shown in (H) and corresponding representative images are provided in fig. S9C. ( I to K ) A total of 5 × 10 6 MDA-MB-231 cells stably expressing shNC or shSF3A2 #2 were transplanted into the mammary fat pads of mice ( n = 10). Cisplatin treatment (3 mg/kg in 1× PBS) was initiated when tumors reached 100 mm 3 and administered twice a week via intraperitoneal injection. Tumor growth curve (I), tumor photograph (J), and weight (K) are shown. ( L ) The proposed working model.

    Article Snippet: Apoptosis was evaluated using an annexin V-FITC/PI Apoptosis Detection Kit (#40302ES60, Yeasen Biotechnology, Shanghai, China).

    Techniques: Western Blot, Stable Transfection, Expressing, Flow Cytometry, In Vivo, Ubiquitin Assay, Transfection, Plasmid Preparation, Immunofluorescence, Staining, CCK-8 Assay, Colony Assay, Injection