annexin v fitc pi cell apoptosis detection kit  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc annexin v fitc pi cell apoptosis detection kit
    Annexin V Fitc Pi Cell Apoptosis Detection Kit, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/annexin v fitc pi cell apoptosis detection kit/product/Cell Signaling Technology Inc
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    annexin v fitc pi cell apoptosis detection kit - by Bioz Stars, 2023-06
    96/100 stars

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    annexin v fitc pi cell apoptosis detection kit  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc annexin v fitc pi cell apoptosis detection kit
    Autophagy flux changes mediated neurons degenerated and <t>apoptosis</t> after ICH-IVH and MCC950 treatment in SVZ. (a) Representative immunofluorescence staining images of Atg5 and p62 positive neurons in SVZ. Bar = 50 μ m. (b) Western blots images and analysis results showed the expression of autophagy proteins, Atg5, LC3B, and p62, in the SVZ of ICH-IVH rats receiving MCC950 or saline. (c, d) Images of FJC-staining and TUNEL-staining of neurons in SVZ after ICH-IVH and MCC950 treatment (c), analysis results of FJC(+) and TUNEL(+) neurons (d). Bar = 50 μ m. (e) TEM images of neurons located in SVZ showed microstructure and edema. Bar = 2 μ m. Results are presented as mean ± SD, ∗∗ P < 0.01 versus sham group, ## P < 0.01 and # P < 0.05 ICH-IVH group versus MCC950 group.
    Annexin V Fitc Pi Cell Apoptosis Detection Kit, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/annexin v fitc pi cell apoptosis detection kit/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    annexin v fitc pi cell apoptosis detection kit - by Bioz Stars, 2023-06
    86/100 stars

    Images

    1) Product Images from "Inhibiting Microglia-Derived NLRP3 Alleviates Subependymal Edema and Cognitive Dysfunction in Posthemorrhagic Hydrocephalus after Intracerebral Hemorrhage via AMPK/Beclin-1 Pathway"

    Article Title: Inhibiting Microglia-Derived NLRP3 Alleviates Subependymal Edema and Cognitive Dysfunction in Posthemorrhagic Hydrocephalus after Intracerebral Hemorrhage via AMPK/Beclin-1 Pathway

    Journal: Oxidative Medicine and Cellular Longevity

    doi: 10.1155/2022/4177317

    Autophagy flux changes mediated neurons degenerated and apoptosis after ICH-IVH and MCC950 treatment in SVZ. (a) Representative immunofluorescence staining images of Atg5 and p62 positive neurons in SVZ. Bar = 50 μ m. (b) Western blots images and analysis results showed the expression of autophagy proteins, Atg5, LC3B, and p62, in the SVZ of ICH-IVH rats receiving MCC950 or saline. (c, d) Images of FJC-staining and TUNEL-staining of neurons in SVZ after ICH-IVH and MCC950 treatment (c), analysis results of FJC(+) and TUNEL(+) neurons (d). Bar = 50 μ m. (e) TEM images of neurons located in SVZ showed microstructure and edema. Bar = 2 μ m. Results are presented as mean ± SD, ∗∗ P < 0.01 versus sham group, ## P < 0.01 and # P < 0.05 ICH-IVH group versus MCC950 group.
    Figure Legend Snippet: Autophagy flux changes mediated neurons degenerated and apoptosis after ICH-IVH and MCC950 treatment in SVZ. (a) Representative immunofluorescence staining images of Atg5 and p62 positive neurons in SVZ. Bar = 50 μ m. (b) Western blots images and analysis results showed the expression of autophagy proteins, Atg5, LC3B, and p62, in the SVZ of ICH-IVH rats receiving MCC950 or saline. (c, d) Images of FJC-staining and TUNEL-staining of neurons in SVZ after ICH-IVH and MCC950 treatment (c), analysis results of FJC(+) and TUNEL(+) neurons (d). Bar = 50 μ m. (e) TEM images of neurons located in SVZ showed microstructure and edema. Bar = 2 μ m. Results are presented as mean ± SD, ∗∗ P < 0.01 versus sham group, ## P < 0.01 and # P < 0.05 ICH-IVH group versus MCC950 group.

    Techniques Used: Immunofluorescence, Staining, Western Blot, Expressing, TUNEL Assay

    Transcriptional analysis of SVZ tissues identified AMPK/ULK1/Beclin-1 as a potential pathway of the microglia/macrophage-derived NLRP3 inflammasome and neuron excessive autophagy-mediated apoptosis after ICH-IVH. (a) Volcano plot showed differentially expressed genes in SVZ on day 3 after ICH-IVH and MCC950 treatment. (b) Heatmap of the significantly different expression genes identified by PCA for each sample between the sham group and the ICH-IVH group. (c) Heatmap showed obviously different expression genes with MCC950 treatment or not after ICH-IVH. Data were clustered hierarchically in GENE-E and colored according to row minimum and maximum. (d) Representative Western blots images of AMPK/ULK1/Beclin-1 pathway and quantitative analyses results. Data were represented as mean ± SD, ∗∗ P < 0.01 versus sham group, ## P < 0.01 ICH-IVH group versus MCC950 group.
    Figure Legend Snippet: Transcriptional analysis of SVZ tissues identified AMPK/ULK1/Beclin-1 as a potential pathway of the microglia/macrophage-derived NLRP3 inflammasome and neuron excessive autophagy-mediated apoptosis after ICH-IVH. (a) Volcano plot showed differentially expressed genes in SVZ on day 3 after ICH-IVH and MCC950 treatment. (b) Heatmap of the significantly different expression genes identified by PCA for each sample between the sham group and the ICH-IVH group. (c) Heatmap showed obviously different expression genes with MCC950 treatment or not after ICH-IVH. Data were clustered hierarchically in GENE-E and colored according to row minimum and maximum. (d) Representative Western blots images of AMPK/ULK1/Beclin-1 pathway and quantitative analyses results. Data were represented as mean ± SD, ∗∗ P < 0.01 versus sham group, ## P < 0.01 ICH-IVH group versus MCC950 group.

    Techniques Used: Derivative Assay, Expressing, Western Blot

    IL-1beta upregulated neurons autophagy and apoptosis, and inhibiting autophagy could reduce neurons apoptosis mediated by IL-beta which was released after NLRP3 activated. (a) Representative immunofluorescence staining images of Atg5- and p62-positive PC12 cells. Bar = 50 μ m. (b) Immunofluorescence TUNEL-staining images of PC12 cells, and statistic result of TUNEL(+) cells after different treatments. Bar = 50 μ m. (c) Representative Western blot images of LC3B and p62 expressions in PC12 cells, and statistic results of LC3B-II : LC3B-I ratio and p62 after IL-1beta and compound C treated. (d) Representative images and statistic results of flow cytometry showed early/late apoptotic cells after IL-1beta and compound C treated. ∗∗ P < 0.01 versus sham group, ## P < 0.01 IL-1beta group versus IL-1beta + compound C group.
    Figure Legend Snippet: IL-1beta upregulated neurons autophagy and apoptosis, and inhibiting autophagy could reduce neurons apoptosis mediated by IL-beta which was released after NLRP3 activated. (a) Representative immunofluorescence staining images of Atg5- and p62-positive PC12 cells. Bar = 50 μ m. (b) Immunofluorescence TUNEL-staining images of PC12 cells, and statistic result of TUNEL(+) cells after different treatments. Bar = 50 μ m. (c) Representative Western blot images of LC3B and p62 expressions in PC12 cells, and statistic results of LC3B-II : LC3B-I ratio and p62 after IL-1beta and compound C treated. (d) Representative images and statistic results of flow cytometry showed early/late apoptotic cells after IL-1beta and compound C treated. ∗∗ P < 0.01 versus sham group, ## P < 0.01 IL-1beta group versus IL-1beta + compound C group.

    Techniques Used: Immunofluorescence, Staining, TUNEL Assay, Western Blot, Flow Cytometry

    Schematic mechanism of NLRP3 activation in microglia/macrophages contributes to subependymal edema and neurons apoptosis by upregulating autophagy through AMPK/ULK1/Beclin-1 pathway. After ICH-IVH, rats occurred subependymal edema which contributes to cognitive dysfunction. Next, we found NLRP3 inflammasome activation in microglia/macrophage-mediated neurons excessive autophagy, and excessive autophagy caused neuron damage through the AMPK/Beclin-1 pathway. Administration NLRP3 specific inhibitor MCC950 could reduce edema in SVZ and improve neurofunction after hydrocephalus.
    Figure Legend Snippet: Schematic mechanism of NLRP3 activation in microglia/macrophages contributes to subependymal edema and neurons apoptosis by upregulating autophagy through AMPK/ULK1/Beclin-1 pathway. After ICH-IVH, rats occurred subependymal edema which contributes to cognitive dysfunction. Next, we found NLRP3 inflammasome activation in microglia/macrophage-mediated neurons excessive autophagy, and excessive autophagy caused neuron damage through the AMPK/Beclin-1 pathway. Administration NLRP3 specific inhibitor MCC950 could reduce edema in SVZ and improve neurofunction after hydrocephalus.

    Techniques Used: Activation Assay

    annexin v fitc pi cell apoptosis detection kit  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc annexin v fitc pi cell apoptosis detection kit
    Annexin V Fitc Pi Cell Apoptosis Detection Kit, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/annexin v fitc pi cell apoptosis detection kit/product/Cell Signaling Technology Inc
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    annexin v fitc pi cell apoptosis detection kit - by Bioz Stars, 2023-06
    96/100 stars

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    annexin v fitc pi cell apoptosis detection kit  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc annexin v fitc pi cell apoptosis detection kit
    Effect of GB on RA-FLSs proliferation and <t>apoptosis.</t> (A) RA-FLSs were treated with pectolinarin (0, 5, 10, 20, or 30 µM) for 24 or 48 h, then cell viability was evaluated by MTT; (B,C) the apoptosis ratio of RA-FLSs was tested by flow cytometry; (D) the protein levels of cleaved-Caspase-3, Bax and Bcl-2 in RA-FLSs were detected by Western blot. Data were presented as mean ± standard deviation (SD). *, P<0.05, vs. control group. GB, Ginkgolide B; RA-FLS, rheumatoid arthritis fibroblast-like synoviocytes; CIA, collagen II-induced arthritis.
    Annexin V Fitc Pi Cell Apoptosis Detection Kit, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/annexin v fitc pi cell apoptosis detection kit/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    annexin v fitc pi cell apoptosis detection kit - by Bioz Stars, 2023-06
    86/100 stars

    Images

    1) Product Images from "Ginkgolide B attenuates collagen-induced rheumatoid arthritis and regulates fibroblast-like synoviocytes-mediated apoptosis and inflammation"

    Article Title: Ginkgolide B attenuates collagen-induced rheumatoid arthritis and regulates fibroblast-like synoviocytes-mediated apoptosis and inflammation

    Journal: Annals of Translational Medicine

    doi: 10.21037/atm-20-6420

    Effect of GB on RA-FLSs proliferation and apoptosis. (A) RA-FLSs were treated with pectolinarin (0, 5, 10, 20, or 30 µM) for 24 or 48 h, then cell viability was evaluated by MTT; (B,C) the apoptosis ratio of RA-FLSs was tested by flow cytometry; (D) the protein levels of cleaved-Caspase-3, Bax and Bcl-2 in RA-FLSs were detected by Western blot. Data were presented as mean ± standard deviation (SD). *, P<0.05, vs. control group. GB, Ginkgolide B; RA-FLS, rheumatoid arthritis fibroblast-like synoviocytes; CIA, collagen II-induced arthritis.
    Figure Legend Snippet: Effect of GB on RA-FLSs proliferation and apoptosis. (A) RA-FLSs were treated with pectolinarin (0, 5, 10, 20, or 30 µM) for 24 or 48 h, then cell viability was evaluated by MTT; (B,C) the apoptosis ratio of RA-FLSs was tested by flow cytometry; (D) the protein levels of cleaved-Caspase-3, Bax and Bcl-2 in RA-FLSs were detected by Western blot. Data were presented as mean ± standard deviation (SD). *, P<0.05, vs. control group. GB, Ginkgolide B; RA-FLS, rheumatoid arthritis fibroblast-like synoviocytes; CIA, collagen II-induced arthritis.

    Techniques Used: Flow Cytometry, Western Blot, Standard Deviation

    annexin v fitc pi cell apoptosis detection kit  (Cell Signaling Technology Inc)


    Bioz Verified Symbol Cell Signaling Technology Inc is a verified supplier
    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
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    Cell Signaling Technology Inc annexin v fitc pi cell apoptosis detection kit
    Effect of GB on RA-FLSs proliferation and <t>apoptosis.</t> (A) RA-FLSs were treated with pectolinarin (0, 5, 10, 20, or 30 µM) for 24 or 48 h, then cell viability was evaluated by MTT; (B,C) the apoptosis ratio of RA-FLSs was tested by flow cytometry; (D) the protein levels of cleaved-Caspase-3, Bax and Bcl-2 in RA-FLSs were detected by Western blot. Data were presented as mean ± standard deviation (SD). *, P<0.05, vs. control group. GB, Ginkgolide B; RA-FLS, rheumatoid arthritis fibroblast-like synoviocytes; CIA, collagen II-induced arthritis.
    Annexin V Fitc Pi Cell Apoptosis Detection Kit, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/annexin v fitc pi cell apoptosis detection kit/product/Cell Signaling Technology Inc
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    annexin v fitc pi cell apoptosis detection kit - by Bioz Stars, 2023-06
    96/100 stars

    Images

    1) Product Images from "Ginkgolide B attenuates collagen-induced rheumatoid arthritis and regulates fibroblast-like synoviocytes-mediated apoptosis and inflammation"

    Article Title: Ginkgolide B attenuates collagen-induced rheumatoid arthritis and regulates fibroblast-like synoviocytes-mediated apoptosis and inflammation

    Journal: Annals of Translational Medicine

    doi: 10.21037/atm-20-6420

    Effect of GB on RA-FLSs proliferation and apoptosis. (A) RA-FLSs were treated with pectolinarin (0, 5, 10, 20, or 30 µM) for 24 or 48 h, then cell viability was evaluated by MTT; (B,C) the apoptosis ratio of RA-FLSs was tested by flow cytometry; (D) the protein levels of cleaved-Caspase-3, Bax and Bcl-2 in RA-FLSs were detected by Western blot. Data were presented as mean ± standard deviation (SD). *, P<0.05, vs. control group. GB, Ginkgolide B; RA-FLS, rheumatoid arthritis fibroblast-like synoviocytes; CIA, collagen II-induced arthritis.
    Figure Legend Snippet: Effect of GB on RA-FLSs proliferation and apoptosis. (A) RA-FLSs were treated with pectolinarin (0, 5, 10, 20, or 30 µM) for 24 or 48 h, then cell viability was evaluated by MTT; (B,C) the apoptosis ratio of RA-FLSs was tested by flow cytometry; (D) the protein levels of cleaved-Caspase-3, Bax and Bcl-2 in RA-FLSs were detected by Western blot. Data were presented as mean ± standard deviation (SD). *, P<0.05, vs. control group. GB, Ginkgolide B; RA-FLS, rheumatoid arthritis fibroblast-like synoviocytes; CIA, collagen II-induced arthritis.

    Techniques Used: Flow Cytometry, Western Blot, Standard Deviation

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    Cell Signaling Technology Inc annexin v fitc pi cell apoptosis detection kit
    Annexin V Fitc Pi Cell Apoptosis Detection Kit, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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