annexin v fitc pi apoptosis detection kit  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc annexin v fitc pi apoptosis detection kit
    Annexin V Fitc Pi Apoptosis Detection Kit, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    annexin v fitc pi apoptosis detection kit  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc annexin v fitc pi apoptosis detection kit
    Induction of <t>apoptosis</t> in HepG2 cells by <t>annexin-V/propidium</t> iodide stain. Cytograms showing HepG2 cells treated with Pitavastatin (1.84 µM, 48 h) ( A ), HepG2 cells as an untreated control ( B ).
    Annexin V Fitc Pi Apoptosis Detection Kit, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Exploring a repurposed candidate with dual hIDO1/hTDO2 inhibitory potential for anticancer efficacy identified through pharmacophore-based virtual screening and in vitro evaluation"

    Article Title: Exploring a repurposed candidate with dual hIDO1/hTDO2 inhibitory potential for anticancer efficacy identified through pharmacophore-based virtual screening and in vitro evaluation

    Journal: Scientific Reports

    doi: 10.1038/s41598-024-59353-4

    Induction of apoptosis in HepG2 cells by annexin-V/propidium iodide stain. Cytograms showing HepG2 cells treated with Pitavastatin (1.84 µM, 48 h) ( A ), HepG2 cells as an untreated control ( B ).
    Figure Legend Snippet: Induction of apoptosis in HepG2 cells by annexin-V/propidium iodide stain. Cytograms showing HepG2 cells treated with Pitavastatin (1.84 µM, 48 h) ( A ), HepG2 cells as an untreated control ( B ).

    Techniques Used: Staining

    biomedicine pharmacotherapy 168 2023 115757 annexin v fitc pi apoptosis detection kit  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc biomedicine pharmacotherapy 168 2023 115757 annexin v fitc pi apoptosis detection kit
    Biomedicine Pharmacotherapy 168 2023 115757 Annexin V Fitc Pi Apoptosis Detection Kit, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biomedicine pharmacotherapy 168 2023 115757 annexin v fitc pi apoptosis detection kit/product/Cell Signaling Technology Inc
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    annexin v fitc pi apoptosis detection kit  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc annexin v fitc pi apoptosis detection kit
    Annexin V Fitc Pi Apoptosis Detection Kit, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/annexin v fitc pi apoptosis detection kit/product/Cell Signaling Technology Inc
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    annexin v fitc propidium iodide pi apoptosis detection kit  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc annexin v fitc propidium iodide pi apoptosis detection kit
    Annexin V Fitc Propidium Iodide Pi Apoptosis Detection Kit, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/annexin v fitc propidium iodide pi apoptosis detection kit/product/Cell Signaling Technology Inc
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    annexin v fitc pi apoptosis detection kit  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc annexin v fitc pi apoptosis detection kit
    (A) In vitro cytotoxicity assay showing MTT activity across 9 days of induction in different culture conditions. Error bars represents mean ± S.D. The differences in cell proliferation between the groups were considered statistically significant at p <0.05 and p -values are indicated on the graph. (*) indicates significant increase in cell proliferation at day 9 compared to day 3 and day 6 in 5% patient sera group. (#) indicates no significant difference in hMSC proliferation at different time points across 9 days of culture. (B) Cell proliferation assay by direct cell counting reveals higher proliferation rate in 10% FBS, which was comparable to that of 10% normal sera (NS) induction group. 10% patient sera (PS) induction group did not show any increase in cell proliferation. However, 5% PS group showed increase in proliferation, which was significantly more compared to 10% PS group (* p <0.05). (C) Cell death assay by <t>annexinV-FITC/PI</t> flow cytometric quantification revealed that 10% patient sera caused mostly necrotic cell death with increase in cell death across 9 days, whereas in 5% PS group cell death was comparably minimal. However, there was negligible cell death in control groups.
    Annexin V Fitc Pi Apoptosis Detection Kit, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "In Vitro Hepatic Trans-Differentiation of Human Mesenchymal Stem Cells Using Sera from Congestive/Ischemic Liver during Cardiac Failure"

    Article Title: In Vitro Hepatic Trans-Differentiation of Human Mesenchymal Stem Cells Using Sera from Congestive/Ischemic Liver during Cardiac Failure

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0092397

    (A) In vitro cytotoxicity assay showing MTT activity across 9 days of induction in different culture conditions. Error bars represents mean ± S.D. The differences in cell proliferation between the groups were considered statistically significant at p <0.05 and p -values are indicated on the graph. (*) indicates significant increase in cell proliferation at day 9 compared to day 3 and day 6 in 5% patient sera group. (#) indicates no significant difference in hMSC proliferation at different time points across 9 days of culture. (B) Cell proliferation assay by direct cell counting reveals higher proliferation rate in 10% FBS, which was comparable to that of 10% normal sera (NS) induction group. 10% patient sera (PS) induction group did not show any increase in cell proliferation. However, 5% PS group showed increase in proliferation, which was significantly more compared to 10% PS group (* p <0.05). (C) Cell death assay by annexinV-FITC/PI flow cytometric quantification revealed that 10% patient sera caused mostly necrotic cell death with increase in cell death across 9 days, whereas in 5% PS group cell death was comparably minimal. However, there was negligible cell death in control groups.
    Figure Legend Snippet: (A) In vitro cytotoxicity assay showing MTT activity across 9 days of induction in different culture conditions. Error bars represents mean ± S.D. The differences in cell proliferation between the groups were considered statistically significant at p <0.05 and p -values are indicated on the graph. (*) indicates significant increase in cell proliferation at day 9 compared to day 3 and day 6 in 5% patient sera group. (#) indicates no significant difference in hMSC proliferation at different time points across 9 days of culture. (B) Cell proliferation assay by direct cell counting reveals higher proliferation rate in 10% FBS, which was comparable to that of 10% normal sera (NS) induction group. 10% patient sera (PS) induction group did not show any increase in cell proliferation. However, 5% PS group showed increase in proliferation, which was significantly more compared to 10% PS group (* p <0.05). (C) Cell death assay by annexinV-FITC/PI flow cytometric quantification revealed that 10% patient sera caused mostly necrotic cell death with increase in cell death across 9 days, whereas in 5% PS group cell death was comparably minimal. However, there was negligible cell death in control groups.

    Techniques Used: In Vitro, Cytotoxicity Assay, Activity Assay, Proliferation Assay, Cell Counting

    annexin v fitc pi apoptosis detection kit  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc annexin v fitc pi apoptosis detection kit
    Annexin V Fitc Pi Apoptosis Detection Kit, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/annexin v fitc pi apoptosis detection kit/product/Cell Signaling Technology Inc
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    pi kit  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc pi kit
    Pi Kit, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pi kit/product/Cell Signaling Technology Inc
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    annexin v fitc pi apoptosis kit  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc annexin v fitc pi apoptosis kit
    (a) Dual-fluorescence flow cytometry cellometer image cytometer (K2) was used to detect the change of apoptotic rate of CNE2 cells after isoimperatorin intervention. The apoptotic rate in the 10 μ M group was 23.06 ± 2.00%, the 20 μ M group was 22.90 ± 3.47%, and the 40 μ M group was 29.06 ± 1.25% vs control group: ∗∗ P < 0.01. Cytation™ 5 cell imaging multifunctional detection system detects changes in the nucleus (b) and cell membrane potentials (c) of the cells after the intervention of isoimperatorin. (A) Control; (B)ISOIMP 10 μ M; (C) ISOIMP20 μ M; (D)ISOIMP 40 μ M; (E) CIS 4.0 μ g·mL –1 (bar=100 μ m, 200x). The nucleus appears bright blue with nuclear condensation. The increase in green fluorescence indicates a decrease in CNE2 cell membrane potentials and cell <t>apoptosis.</t> Isoimperatorin inhibits the expression of proliferation-related and apoptosis-related proteins in CNE2 cells. Western blot analysis of the effects of isoimperatorin at different concentrations on the relative expression of PCNA, XIAP, and survivin (d) and Bax and Bcl-2 (e) in CNE2 cells vs control group: ∗ P < 0.05; ∗∗ P < 0.01.
    Annexin V Fitc Pi Apoptosis Kit, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/annexin v fitc pi apoptosis kit/product/Cell Signaling Technology Inc
    Average 96 stars, based on 1 article reviews
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    annexin v fitc pi apoptosis kit - by Bioz Stars, 2024-06
    96/100 stars

    Images

    1) Product Images from "Isoimperatorin Induces Apoptosis of Nasopharyngeal Carcinoma Cells via the MAPK/ERK1/2 Signaling Pathway"

    Article Title: Isoimperatorin Induces Apoptosis of Nasopharyngeal Carcinoma Cells via the MAPK/ERK1/2 Signaling Pathway

    Journal: Evidence-based Complementary and Alternative Medicine : eCAM

    doi: 10.1155/2020/2138186

    (a) Dual-fluorescence flow cytometry cellometer image cytometer (K2) was used to detect the change of apoptotic rate of CNE2 cells after isoimperatorin intervention. The apoptotic rate in the 10 μ M group was 23.06 ± 2.00%, the 20 μ M group was 22.90 ± 3.47%, and the 40 μ M group was 29.06 ± 1.25% vs control group: ∗∗ P < 0.01. Cytation™ 5 cell imaging multifunctional detection system detects changes in the nucleus (b) and cell membrane potentials (c) of the cells after the intervention of isoimperatorin. (A) Control; (B)ISOIMP 10 μ M; (C) ISOIMP20 μ M; (D)ISOIMP 40 μ M; (E) CIS 4.0 μ g·mL –1 (bar=100 μ m, 200x). The nucleus appears bright blue with nuclear condensation. The increase in green fluorescence indicates a decrease in CNE2 cell membrane potentials and cell apoptosis. Isoimperatorin inhibits the expression of proliferation-related and apoptosis-related proteins in CNE2 cells. Western blot analysis of the effects of isoimperatorin at different concentrations on the relative expression of PCNA, XIAP, and survivin (d) and Bax and Bcl-2 (e) in CNE2 cells vs control group: ∗ P < 0.05; ∗∗ P < 0.01.
    Figure Legend Snippet: (a) Dual-fluorescence flow cytometry cellometer image cytometer (K2) was used to detect the change of apoptotic rate of CNE2 cells after isoimperatorin intervention. The apoptotic rate in the 10 μ M group was 23.06 ± 2.00%, the 20 μ M group was 22.90 ± 3.47%, and the 40 μ M group was 29.06 ± 1.25% vs control group: ∗∗ P < 0.01. Cytation™ 5 cell imaging multifunctional detection system detects changes in the nucleus (b) and cell membrane potentials (c) of the cells after the intervention of isoimperatorin. (A) Control; (B)ISOIMP 10 μ M; (C) ISOIMP20 μ M; (D)ISOIMP 40 μ M; (E) CIS 4.0 μ g·mL –1 (bar=100 μ m, 200x). The nucleus appears bright blue with nuclear condensation. The increase in green fluorescence indicates a decrease in CNE2 cell membrane potentials and cell apoptosis. Isoimperatorin inhibits the expression of proliferation-related and apoptosis-related proteins in CNE2 cells. Western blot analysis of the effects of isoimperatorin at different concentrations on the relative expression of PCNA, XIAP, and survivin (d) and Bax and Bcl-2 (e) in CNE2 cells vs control group: ∗ P < 0.05; ∗∗ P < 0.01.

    Techniques Used: Fluorescence, Flow Cytometry, Cytometry, Imaging, Expressing, Western Blot

    Effect of isoimperatorin on CNE2 cell apoptosis is attenuated by ISO. Western blot analysis shows the expression of p-c-RAF, p-MEK, and p-ERK1/2 (a), PCNA, XIAP, and survivin (b), and Bax and Bcl-2 (c) in CNE2 cells. vs control group: ∗∗ P < 0.01; vs ISOIMP group, # P < 0.05; ## P < 0.01; dual-fluorescence flow cytometry cellometer image cytometer (K2) was used to detect the change of apoptotic rate of CNE2 cells after ISO and isoimperatorin intervention (d) vs control group: ∗∗ P < 0.01; vs ISOIMP group, # P < 0.05; ## P < 0.01.
    Figure Legend Snippet: Effect of isoimperatorin on CNE2 cell apoptosis is attenuated by ISO. Western blot analysis shows the expression of p-c-RAF, p-MEK, and p-ERK1/2 (a), PCNA, XIAP, and survivin (b), and Bax and Bcl-2 (c) in CNE2 cells. vs control group: ∗∗ P < 0.01; vs ISOIMP group, # P < 0.05; ## P < 0.01; dual-fluorescence flow cytometry cellometer image cytometer (K2) was used to detect the change of apoptotic rate of CNE2 cells after ISO and isoimperatorin intervention (d) vs control group: ∗∗ P < 0.01; vs ISOIMP group, # P < 0.05; ## P < 0.01.

    Techniques Used: Western Blot, Expressing, Fluorescence, Flow Cytometry, Cytometry

    annexin v fitc pi cell apoptosis detection kit  (Cell Signaling Technology Inc)


    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
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    Cell Signaling Technology Inc annexin v fitc pi cell apoptosis detection kit
    Autophagy flux changes mediated neurons degenerated and <t>apoptosis</t> after ICH-IVH and MCC950 treatment in SVZ. (a) Representative immunofluorescence staining images of Atg5 and p62 positive neurons in SVZ. Bar = 50 μ m. (b) Western blots images and analysis results showed the expression of autophagy proteins, Atg5, LC3B, and p62, in the SVZ of ICH-IVH rats receiving MCC950 or saline. (c, d) Images of FJC-staining and TUNEL-staining of neurons in SVZ after ICH-IVH and MCC950 treatment (c), analysis results of FJC(+) and TUNEL(+) neurons (d). Bar = 50 μ m. (e) TEM images of neurons located in SVZ showed microstructure and edema. Bar = 2 μ m. Results are presented as mean ± SD, ∗∗ P < 0.01 versus sham group, ## P < 0.01 and # P < 0.05 ICH-IVH group versus MCC950 group.
    Annexin V Fitc Pi Cell Apoptosis Detection Kit, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "Inhibiting Microglia-Derived NLRP3 Alleviates Subependymal Edema and Cognitive Dysfunction in Posthemorrhagic Hydrocephalus after Intracerebral Hemorrhage via AMPK/Beclin-1 Pathway"

    Article Title: Inhibiting Microglia-Derived NLRP3 Alleviates Subependymal Edema and Cognitive Dysfunction in Posthemorrhagic Hydrocephalus after Intracerebral Hemorrhage via AMPK/Beclin-1 Pathway

    Journal: Oxidative Medicine and Cellular Longevity

    doi: 10.1155/2022/4177317

    Autophagy flux changes mediated neurons degenerated and apoptosis after ICH-IVH and MCC950 treatment in SVZ. (a) Representative immunofluorescence staining images of Atg5 and p62 positive neurons in SVZ. Bar = 50 μ m. (b) Western blots images and analysis results showed the expression of autophagy proteins, Atg5, LC3B, and p62, in the SVZ of ICH-IVH rats receiving MCC950 or saline. (c, d) Images of FJC-staining and TUNEL-staining of neurons in SVZ after ICH-IVH and MCC950 treatment (c), analysis results of FJC(+) and TUNEL(+) neurons (d). Bar = 50 μ m. (e) TEM images of neurons located in SVZ showed microstructure and edema. Bar = 2 μ m. Results are presented as mean ± SD, ∗∗ P < 0.01 versus sham group, ## P < 0.01 and # P < 0.05 ICH-IVH group versus MCC950 group.
    Figure Legend Snippet: Autophagy flux changes mediated neurons degenerated and apoptosis after ICH-IVH and MCC950 treatment in SVZ. (a) Representative immunofluorescence staining images of Atg5 and p62 positive neurons in SVZ. Bar = 50 μ m. (b) Western blots images and analysis results showed the expression of autophagy proteins, Atg5, LC3B, and p62, in the SVZ of ICH-IVH rats receiving MCC950 or saline. (c, d) Images of FJC-staining and TUNEL-staining of neurons in SVZ after ICH-IVH and MCC950 treatment (c), analysis results of FJC(+) and TUNEL(+) neurons (d). Bar = 50 μ m. (e) TEM images of neurons located in SVZ showed microstructure and edema. Bar = 2 μ m. Results are presented as mean ± SD, ∗∗ P < 0.01 versus sham group, ## P < 0.01 and # P < 0.05 ICH-IVH group versus MCC950 group.

    Techniques Used: Immunofluorescence, Staining, Western Blot, Expressing, TUNEL Assay

    Transcriptional analysis of SVZ tissues identified AMPK/ULK1/Beclin-1 as a potential pathway of the microglia/macrophage-derived NLRP3 inflammasome and neuron excessive autophagy-mediated apoptosis after ICH-IVH. (a) Volcano plot showed differentially expressed genes in SVZ on day 3 after ICH-IVH and MCC950 treatment. (b) Heatmap of the significantly different expression genes identified by PCA for each sample between the sham group and the ICH-IVH group. (c) Heatmap showed obviously different expression genes with MCC950 treatment or not after ICH-IVH. Data were clustered hierarchically in GENE-E and colored according to row minimum and maximum. (d) Representative Western blots images of AMPK/ULK1/Beclin-1 pathway and quantitative analyses results. Data were represented as mean ± SD, ∗∗ P < 0.01 versus sham group, ## P < 0.01 ICH-IVH group versus MCC950 group.
    Figure Legend Snippet: Transcriptional analysis of SVZ tissues identified AMPK/ULK1/Beclin-1 as a potential pathway of the microglia/macrophage-derived NLRP3 inflammasome and neuron excessive autophagy-mediated apoptosis after ICH-IVH. (a) Volcano plot showed differentially expressed genes in SVZ on day 3 after ICH-IVH and MCC950 treatment. (b) Heatmap of the significantly different expression genes identified by PCA for each sample between the sham group and the ICH-IVH group. (c) Heatmap showed obviously different expression genes with MCC950 treatment or not after ICH-IVH. Data were clustered hierarchically in GENE-E and colored according to row minimum and maximum. (d) Representative Western blots images of AMPK/ULK1/Beclin-1 pathway and quantitative analyses results. Data were represented as mean ± SD, ∗∗ P < 0.01 versus sham group, ## P < 0.01 ICH-IVH group versus MCC950 group.

    Techniques Used: Derivative Assay, Expressing, Western Blot

    IL-1beta upregulated neurons autophagy and apoptosis, and inhibiting autophagy could reduce neurons apoptosis mediated by IL-beta which was released after NLRP3 activated. (a) Representative immunofluorescence staining images of Atg5- and p62-positive PC12 cells. Bar = 50 μ m. (b) Immunofluorescence TUNEL-staining images of PC12 cells, and statistic result of TUNEL(+) cells after different treatments. Bar = 50 μ m. (c) Representative Western blot images of LC3B and p62 expressions in PC12 cells, and statistic results of LC3B-II : LC3B-I ratio and p62 after IL-1beta and compound C treated. (d) Representative images and statistic results of flow cytometry showed early/late apoptotic cells after IL-1beta and compound C treated. ∗∗ P < 0.01 versus sham group, ## P < 0.01 IL-1beta group versus IL-1beta + compound C group.
    Figure Legend Snippet: IL-1beta upregulated neurons autophagy and apoptosis, and inhibiting autophagy could reduce neurons apoptosis mediated by IL-beta which was released after NLRP3 activated. (a) Representative immunofluorescence staining images of Atg5- and p62-positive PC12 cells. Bar = 50 μ m. (b) Immunofluorescence TUNEL-staining images of PC12 cells, and statistic result of TUNEL(+) cells after different treatments. Bar = 50 μ m. (c) Representative Western blot images of LC3B and p62 expressions in PC12 cells, and statistic results of LC3B-II : LC3B-I ratio and p62 after IL-1beta and compound C treated. (d) Representative images and statistic results of flow cytometry showed early/late apoptotic cells after IL-1beta and compound C treated. ∗∗ P < 0.01 versus sham group, ## P < 0.01 IL-1beta group versus IL-1beta + compound C group.

    Techniques Used: Immunofluorescence, Staining, TUNEL Assay, Western Blot, Flow Cytometry

    Schematic mechanism of NLRP3 activation in microglia/macrophages contributes to subependymal edema and neurons apoptosis by upregulating autophagy through AMPK/ULK1/Beclin-1 pathway. After ICH-IVH, rats occurred subependymal edema which contributes to cognitive dysfunction. Next, we found NLRP3 inflammasome activation in microglia/macrophage-mediated neurons excessive autophagy, and excessive autophagy caused neuron damage through the AMPK/Beclin-1 pathway. Administration NLRP3 specific inhibitor MCC950 could reduce edema in SVZ and improve neurofunction after hydrocephalus.
    Figure Legend Snippet: Schematic mechanism of NLRP3 activation in microglia/macrophages contributes to subependymal edema and neurons apoptosis by upregulating autophagy through AMPK/ULK1/Beclin-1 pathway. After ICH-IVH, rats occurred subependymal edema which contributes to cognitive dysfunction. Next, we found NLRP3 inflammasome activation in microglia/macrophage-mediated neurons excessive autophagy, and excessive autophagy caused neuron damage through the AMPK/Beclin-1 pathway. Administration NLRP3 specific inhibitor MCC950 could reduce edema in SVZ and improve neurofunction after hydrocephalus.

    Techniques Used: Activation Assay

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    Cell Signaling Technology Inc annexin v fitc pi apoptosis detection kit
    Annexin V Fitc Pi Apoptosis Detection Kit, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    (a) Dual-fluorescence flow cytometry cellometer image cytometer (K2) was used to detect the change of apoptotic rate of CNE2 cells after isoimperatorin intervention. The apoptotic rate in the 10 μ M group was 23.06 ± 2.00%, the 20 μ M group was 22.90 ± 3.47%, and the 40 μ M group was 29.06 ± 1.25% vs control group: ∗∗ P < 0.01. Cytation™ 5 cell imaging multifunctional detection system detects changes in the nucleus (b) and cell membrane potentials (c) of the cells after the intervention of isoimperatorin. (A) Control; (B)ISOIMP 10 μ M; (C) ISOIMP20 μ M; (D)ISOIMP 40 μ M; (E) CIS 4.0 μ g·mL –1 (bar=100 μ m, 200x). The nucleus appears bright blue with nuclear condensation. The increase in green fluorescence indicates a decrease in CNE2 cell membrane potentials and cell <t>apoptosis.</t> Isoimperatorin inhibits the expression of proliferation-related and apoptosis-related proteins in CNE2 cells. Western blot analysis of the effects of isoimperatorin at different concentrations on the relative expression of PCNA, XIAP, and survivin (d) and Bax and Bcl-2 (e) in CNE2 cells vs control group: ∗ P < 0.05; ∗∗ P < 0.01.
    Annexin V Fitc Pi Apoptosis Kit, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Cell Signaling Technology Inc annexin v fitc pi cell apoptosis detection kit
    Autophagy flux changes mediated neurons degenerated and <t>apoptosis</t> after ICH-IVH and MCC950 treatment in SVZ. (a) Representative immunofluorescence staining images of Atg5 and p62 positive neurons in SVZ. Bar = 50 μ m. (b) Western blots images and analysis results showed the expression of autophagy proteins, Atg5, LC3B, and p62, in the SVZ of ICH-IVH rats receiving MCC950 or saline. (c, d) Images of FJC-staining and TUNEL-staining of neurons in SVZ after ICH-IVH and MCC950 treatment (c), analysis results of FJC(+) and TUNEL(+) neurons (d). Bar = 50 μ m. (e) TEM images of neurons located in SVZ showed microstructure and edema. Bar = 2 μ m. Results are presented as mean ± SD, ∗∗ P < 0.01 versus sham group, ## P < 0.01 and # P < 0.05 ICH-IVH group versus MCC950 group.
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    Image Search Results


    (a) Dual-fluorescence flow cytometry cellometer image cytometer (K2) was used to detect the change of apoptotic rate of CNE2 cells after isoimperatorin intervention. The apoptotic rate in the 10 μ M group was 23.06 ± 2.00%, the 20 μ M group was 22.90 ± 3.47%, and the 40 μ M group was 29.06 ± 1.25% vs control group: ∗∗ P < 0.01. Cytation™ 5 cell imaging multifunctional detection system detects changes in the nucleus (b) and cell membrane potentials (c) of the cells after the intervention of isoimperatorin. (A) Control; (B)ISOIMP 10 μ M; (C) ISOIMP20 μ M; (D)ISOIMP 40 μ M; (E) CIS 4.0 μ g·mL –1 (bar=100 μ m, 200x). The nucleus appears bright blue with nuclear condensation. The increase in green fluorescence indicates a decrease in CNE2 cell membrane potentials and cell apoptosis. Isoimperatorin inhibits the expression of proliferation-related and apoptosis-related proteins in CNE2 cells. Western blot analysis of the effects of isoimperatorin at different concentrations on the relative expression of PCNA, XIAP, and survivin (d) and Bax and Bcl-2 (e) in CNE2 cells vs control group: ∗ P < 0.05; ∗∗ P < 0.01.

    Journal: Evidence-based Complementary and Alternative Medicine : eCAM

    Article Title: Isoimperatorin Induces Apoptosis of Nasopharyngeal Carcinoma Cells via the MAPK/ERK1/2 Signaling Pathway

    doi: 10.1155/2020/2138186

    Figure Lengend Snippet: (a) Dual-fluorescence flow cytometry cellometer image cytometer (K2) was used to detect the change of apoptotic rate of CNE2 cells after isoimperatorin intervention. The apoptotic rate in the 10 μ M group was 23.06 ± 2.00%, the 20 μ M group was 22.90 ± 3.47%, and the 40 μ M group was 29.06 ± 1.25% vs control group: ∗∗ P < 0.01. Cytation™ 5 cell imaging multifunctional detection system detects changes in the nucleus (b) and cell membrane potentials (c) of the cells after the intervention of isoimperatorin. (A) Control; (B)ISOIMP 10 μ M; (C) ISOIMP20 μ M; (D)ISOIMP 40 μ M; (E) CIS 4.0 μ g·mL –1 (bar=100 μ m, 200x). The nucleus appears bright blue with nuclear condensation. The increase in green fluorescence indicates a decrease in CNE2 cell membrane potentials and cell apoptosis. Isoimperatorin inhibits the expression of proliferation-related and apoptosis-related proteins in CNE2 cells. Western blot analysis of the effects of isoimperatorin at different concentrations on the relative expression of PCNA, XIAP, and survivin (d) and Bax and Bcl-2 (e) in CNE2 cells vs control group: ∗ P < 0.05; ∗∗ P < 0.01.

    Article Snippet: RPMI-1640 medium (Hyclone), fetal bovine serum (Gibco), DMSO (Amresco), MTT (Biosharp), Annexin V-FITC/PI apoptosis kit (MULTI SCIENCES), β -actin Mouse mAb, Survivin Rabbit mAb, XIAP Rabbit mAb, Phospho-c-Raf Rabbit mAb, Phospho-MEK Rabbit mAb, Phospho-p44/42 MAPK (ERK1/2) Rabbit mAb (Cell Signaling TECHNOLOGY), Rabbit Anti-PCNA Polyclonal Antibody, Rabbit Anti-Bax polyclonal antibody, and Rabbit Anti-Bcl-2 Polyclonal Antibody (Bioss) were used.

    Techniques: Fluorescence, Flow Cytometry, Cytometry, Imaging, Expressing, Western Blot

    Effect of isoimperatorin on CNE2 cell apoptosis is attenuated by ISO. Western blot analysis shows the expression of p-c-RAF, p-MEK, and p-ERK1/2 (a), PCNA, XIAP, and survivin (b), and Bax and Bcl-2 (c) in CNE2 cells. vs control group: ∗∗ P < 0.01; vs ISOIMP group, # P < 0.05; ## P < 0.01; dual-fluorescence flow cytometry cellometer image cytometer (K2) was used to detect the change of apoptotic rate of CNE2 cells after ISO and isoimperatorin intervention (d) vs control group: ∗∗ P < 0.01; vs ISOIMP group, # P < 0.05; ## P < 0.01.

    Journal: Evidence-based Complementary and Alternative Medicine : eCAM

    Article Title: Isoimperatorin Induces Apoptosis of Nasopharyngeal Carcinoma Cells via the MAPK/ERK1/2 Signaling Pathway

    doi: 10.1155/2020/2138186

    Figure Lengend Snippet: Effect of isoimperatorin on CNE2 cell apoptosis is attenuated by ISO. Western blot analysis shows the expression of p-c-RAF, p-MEK, and p-ERK1/2 (a), PCNA, XIAP, and survivin (b), and Bax and Bcl-2 (c) in CNE2 cells. vs control group: ∗∗ P < 0.01; vs ISOIMP group, # P < 0.05; ## P < 0.01; dual-fluorescence flow cytometry cellometer image cytometer (K2) was used to detect the change of apoptotic rate of CNE2 cells after ISO and isoimperatorin intervention (d) vs control group: ∗∗ P < 0.01; vs ISOIMP group, # P < 0.05; ## P < 0.01.

    Article Snippet: RPMI-1640 medium (Hyclone), fetal bovine serum (Gibco), DMSO (Amresco), MTT (Biosharp), Annexin V-FITC/PI apoptosis kit (MULTI SCIENCES), β -actin Mouse mAb, Survivin Rabbit mAb, XIAP Rabbit mAb, Phospho-c-Raf Rabbit mAb, Phospho-MEK Rabbit mAb, Phospho-p44/42 MAPK (ERK1/2) Rabbit mAb (Cell Signaling TECHNOLOGY), Rabbit Anti-PCNA Polyclonal Antibody, Rabbit Anti-Bax polyclonal antibody, and Rabbit Anti-Bcl-2 Polyclonal Antibody (Bioss) were used.

    Techniques: Western Blot, Expressing, Fluorescence, Flow Cytometry, Cytometry

    Autophagy flux changes mediated neurons degenerated and apoptosis after ICH-IVH and MCC950 treatment in SVZ. (a) Representative immunofluorescence staining images of Atg5 and p62 positive neurons in SVZ. Bar = 50 μ m. (b) Western blots images and analysis results showed the expression of autophagy proteins, Atg5, LC3B, and p62, in the SVZ of ICH-IVH rats receiving MCC950 or saline. (c, d) Images of FJC-staining and TUNEL-staining of neurons in SVZ after ICH-IVH and MCC950 treatment (c), analysis results of FJC(+) and TUNEL(+) neurons (d). Bar = 50 μ m. (e) TEM images of neurons located in SVZ showed microstructure and edema. Bar = 2 μ m. Results are presented as mean ± SD, ∗∗ P < 0.01 versus sham group, ## P < 0.01 and # P < 0.05 ICH-IVH group versus MCC950 group.

    Journal: Oxidative Medicine and Cellular Longevity

    Article Title: Inhibiting Microglia-Derived NLRP3 Alleviates Subependymal Edema and Cognitive Dysfunction in Posthemorrhagic Hydrocephalus after Intracerebral Hemorrhage via AMPK/Beclin-1 Pathway

    doi: 10.1155/2022/4177317

    Figure Lengend Snippet: Autophagy flux changes mediated neurons degenerated and apoptosis after ICH-IVH and MCC950 treatment in SVZ. (a) Representative immunofluorescence staining images of Atg5 and p62 positive neurons in SVZ. Bar = 50 μ m. (b) Western blots images and analysis results showed the expression of autophagy proteins, Atg5, LC3B, and p62, in the SVZ of ICH-IVH rats receiving MCC950 or saline. (c, d) Images of FJC-staining and TUNEL-staining of neurons in SVZ after ICH-IVH and MCC950 treatment (c), analysis results of FJC(+) and TUNEL(+) neurons (d). Bar = 50 μ m. (e) TEM images of neurons located in SVZ showed microstructure and edema. Bar = 2 μ m. Results are presented as mean ± SD, ∗∗ P < 0.01 versus sham group, ## P < 0.01 and # P < 0.05 ICH-IVH group versus MCC950 group.

    Article Snippet: Apoptosis was detected using ANNEXIN V-FITC/PI cell apoptosis detection kit (CST, USA).

    Techniques: Immunofluorescence, Staining, Western Blot, Expressing, TUNEL Assay

    Transcriptional analysis of SVZ tissues identified AMPK/ULK1/Beclin-1 as a potential pathway of the microglia/macrophage-derived NLRP3 inflammasome and neuron excessive autophagy-mediated apoptosis after ICH-IVH. (a) Volcano plot showed differentially expressed genes in SVZ on day 3 after ICH-IVH and MCC950 treatment. (b) Heatmap of the significantly different expression genes identified by PCA for each sample between the sham group and the ICH-IVH group. (c) Heatmap showed obviously different expression genes with MCC950 treatment or not after ICH-IVH. Data were clustered hierarchically in GENE-E and colored according to row minimum and maximum. (d) Representative Western blots images of AMPK/ULK1/Beclin-1 pathway and quantitative analyses results. Data were represented as mean ± SD, ∗∗ P < 0.01 versus sham group, ## P < 0.01 ICH-IVH group versus MCC950 group.

    Journal: Oxidative Medicine and Cellular Longevity

    Article Title: Inhibiting Microglia-Derived NLRP3 Alleviates Subependymal Edema and Cognitive Dysfunction in Posthemorrhagic Hydrocephalus after Intracerebral Hemorrhage via AMPK/Beclin-1 Pathway

    doi: 10.1155/2022/4177317

    Figure Lengend Snippet: Transcriptional analysis of SVZ tissues identified AMPK/ULK1/Beclin-1 as a potential pathway of the microglia/macrophage-derived NLRP3 inflammasome and neuron excessive autophagy-mediated apoptosis after ICH-IVH. (a) Volcano plot showed differentially expressed genes in SVZ on day 3 after ICH-IVH and MCC950 treatment. (b) Heatmap of the significantly different expression genes identified by PCA for each sample between the sham group and the ICH-IVH group. (c) Heatmap showed obviously different expression genes with MCC950 treatment or not after ICH-IVH. Data were clustered hierarchically in GENE-E and colored according to row minimum and maximum. (d) Representative Western blots images of AMPK/ULK1/Beclin-1 pathway and quantitative analyses results. Data were represented as mean ± SD, ∗∗ P < 0.01 versus sham group, ## P < 0.01 ICH-IVH group versus MCC950 group.

    Article Snippet: Apoptosis was detected using ANNEXIN V-FITC/PI cell apoptosis detection kit (CST, USA).

    Techniques: Derivative Assay, Expressing, Western Blot

    IL-1beta upregulated neurons autophagy and apoptosis, and inhibiting autophagy could reduce neurons apoptosis mediated by IL-beta which was released after NLRP3 activated. (a) Representative immunofluorescence staining images of Atg5- and p62-positive PC12 cells. Bar = 50 μ m. (b) Immunofluorescence TUNEL-staining images of PC12 cells, and statistic result of TUNEL(+) cells after different treatments. Bar = 50 μ m. (c) Representative Western blot images of LC3B and p62 expressions in PC12 cells, and statistic results of LC3B-II : LC3B-I ratio and p62 after IL-1beta and compound C treated. (d) Representative images and statistic results of flow cytometry showed early/late apoptotic cells after IL-1beta and compound C treated. ∗∗ P < 0.01 versus sham group, ## P < 0.01 IL-1beta group versus IL-1beta + compound C group.

    Journal: Oxidative Medicine and Cellular Longevity

    Article Title: Inhibiting Microglia-Derived NLRP3 Alleviates Subependymal Edema and Cognitive Dysfunction in Posthemorrhagic Hydrocephalus after Intracerebral Hemorrhage via AMPK/Beclin-1 Pathway

    doi: 10.1155/2022/4177317

    Figure Lengend Snippet: IL-1beta upregulated neurons autophagy and apoptosis, and inhibiting autophagy could reduce neurons apoptosis mediated by IL-beta which was released after NLRP3 activated. (a) Representative immunofluorescence staining images of Atg5- and p62-positive PC12 cells. Bar = 50 μ m. (b) Immunofluorescence TUNEL-staining images of PC12 cells, and statistic result of TUNEL(+) cells after different treatments. Bar = 50 μ m. (c) Representative Western blot images of LC3B and p62 expressions in PC12 cells, and statistic results of LC3B-II : LC3B-I ratio and p62 after IL-1beta and compound C treated. (d) Representative images and statistic results of flow cytometry showed early/late apoptotic cells after IL-1beta and compound C treated. ∗∗ P < 0.01 versus sham group, ## P < 0.01 IL-1beta group versus IL-1beta + compound C group.

    Article Snippet: Apoptosis was detected using ANNEXIN V-FITC/PI cell apoptosis detection kit (CST, USA).

    Techniques: Immunofluorescence, Staining, TUNEL Assay, Western Blot, Flow Cytometry

    Schematic mechanism of NLRP3 activation in microglia/macrophages contributes to subependymal edema and neurons apoptosis by upregulating autophagy through AMPK/ULK1/Beclin-1 pathway. After ICH-IVH, rats occurred subependymal edema which contributes to cognitive dysfunction. Next, we found NLRP3 inflammasome activation in microglia/macrophage-mediated neurons excessive autophagy, and excessive autophagy caused neuron damage through the AMPK/Beclin-1 pathway. Administration NLRP3 specific inhibitor MCC950 could reduce edema in SVZ and improve neurofunction after hydrocephalus.

    Journal: Oxidative Medicine and Cellular Longevity

    Article Title: Inhibiting Microglia-Derived NLRP3 Alleviates Subependymal Edema and Cognitive Dysfunction in Posthemorrhagic Hydrocephalus after Intracerebral Hemorrhage via AMPK/Beclin-1 Pathway

    doi: 10.1155/2022/4177317

    Figure Lengend Snippet: Schematic mechanism of NLRP3 activation in microglia/macrophages contributes to subependymal edema and neurons apoptosis by upregulating autophagy through AMPK/ULK1/Beclin-1 pathway. After ICH-IVH, rats occurred subependymal edema which contributes to cognitive dysfunction. Next, we found NLRP3 inflammasome activation in microglia/macrophage-mediated neurons excessive autophagy, and excessive autophagy caused neuron damage through the AMPK/Beclin-1 pathway. Administration NLRP3 specific inhibitor MCC950 could reduce edema in SVZ and improve neurofunction after hydrocephalus.

    Article Snippet: Apoptosis was detected using ANNEXIN V-FITC/PI cell apoptosis detection kit (CST, USA).

    Techniques: Activation Assay