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( A ) The detection of BCL2, BAX, Cleaved Caspase-3 expression in different groups using western blot. ( B ) The quantification of western blot gel bands in different groups (n=3 independently repeated experiments). ( C ) The gene levels of Bcl2 , Bax , and Bcl2/Bax in different groups (n=5 independently repeated experiments). ( D ) The detection of MIN6 cells <t>apoptosis</t> in each group using TUNEL staining. The cells with red fluorescence represent apoptosis. Scale bar 100 μm. ( E ) The quantitative analysis of TUNEL positive cells in different groups (n=5 independently repeated experiments). ( F ) The apoptosis in each group was analyzed using flow cytometry after Annexin V FITC and PI co-staining. ( G ) The quantitative analysis of flow cytometry after Annexin V FITC and PI co-staining in different groups (n=5 independently repeated experiments). Mean ± SEM. *p<0.05, **p<0.01, ***p<0.001 indicate significant differences, and ns>0.05 means no significance difference. One-way ANOVA. Figure 9—source data 1. Raw unedited gels for . Figure 9—source data 2. Uncropped and labeled gels for .
Annexin V Fitc Pi Apoptosis Detection Kit, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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( A ) The detection of BCL2, BAX, Cleaved Caspase-3 expression in different groups using western blot. ( B ) The quantification of western blot gel bands in different groups (n=3 independently repeated experiments). ( C ) The gene levels of Bcl2 , Bax , and Bcl2/Bax in different groups (n=5 independently repeated experiments). ( D ) The detection of MIN6 cells <t>apoptosis</t> in each group using TUNEL staining. The cells with red fluorescence represent apoptosis. Scale bar 100 μm. ( E ) The quantitative analysis of TUNEL positive cells in different groups (n=5 independently repeated experiments). ( F ) The apoptosis in each group was analyzed using flow cytometry after Annexin V FITC and PI co-staining. ( G ) The quantitative analysis of flow cytometry after Annexin V FITC and PI co-staining in different groups (n=5 independently repeated experiments). Mean ± SEM. *p<0.05, **p<0.01, ***p<0.001 indicate significant differences, and ns>0.05 means no significance difference. One-way ANOVA. Figure 9—source data 1. Raw unedited gels for . Figure 9—source data 2. Uncropped and labeled gels for .
Annexin V‑Fitc Pi Apoptosis Detection Kit, supplied by Vazyme Biotech Co, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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( A ) The detection of BCL2, BAX, Cleaved Caspase-3 expression in different groups using western blot. ( B ) The quantification of western blot gel bands in different groups (n=3 independently repeated experiments). ( C ) The gene levels of Bcl2 , Bax , and Bcl2/Bax in different groups (n=5 independently repeated experiments). ( D ) The detection of MIN6 cells <t>apoptosis</t> in each group using TUNEL staining. The cells with red fluorescence represent apoptosis. Scale bar 100 μm. ( E ) The quantitative analysis of TUNEL positive cells in different groups (n=5 independently repeated experiments). ( F ) The apoptosis in each group was analyzed using flow cytometry after Annexin V FITC and PI co-staining. ( G ) The quantitative analysis of flow cytometry after Annexin V FITC and PI co-staining in different groups (n=5 independently repeated experiments). Mean ± SEM. *p<0.05, **p<0.01, ***p<0.001 indicate significant differences, and ns>0.05 means no significance difference. One-way ANOVA. Figure 9—source data 1. Raw unedited gels for . Figure 9—source data 2. Uncropped and labeled gels for .
Annexin V Fitc Pi Double Staining Apoptosis Detection Kit, supplied by Nanjing KeyGen Biotech Co Ltd, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Vazyme Biotech Co annexin v fitc pi apoptosis detection kit
( A ) The detection of BCL2, BAX, Cleaved Caspase-3 expression in different groups using western blot. ( B ) The quantification of western blot gel bands in different groups (n=3 independently repeated experiments). ( C ) The gene levels of Bcl2 , Bax , and Bcl2/Bax in different groups (n=5 independently repeated experiments). ( D ) The detection of MIN6 cells <t>apoptosis</t> in each group using TUNEL staining. The cells with red fluorescence represent apoptosis. Scale bar 100 μm. ( E ) The quantitative analysis of TUNEL positive cells in different groups (n=5 independently repeated experiments). ( F ) The apoptosis in each group was analyzed using flow cytometry after Annexin V FITC and PI co-staining. ( G ) The quantitative analysis of flow cytometry after Annexin V FITC and PI co-staining in different groups (n=5 independently repeated experiments). Mean ± SEM. *p<0.05, **p<0.01, ***p<0.001 indicate significant differences, and ns>0.05 means no significance difference. One-way ANOVA. Figure 9—source data 1. Raw unedited gels for . Figure 9—source data 2. Uncropped and labeled gels for .
Annexin V Fitc Pi Apoptosis Detection Kit, supplied by Vazyme Biotech Co, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/annexin v fitc pi apoptosis detection kit/product/Vazyme Biotech Co
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annexin v fitc pi apoptosis detection kit - by Bioz Stars, 2025-01
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( A ) The detection of BCL2, BAX, Cleaved Caspase-3 expression in different groups using western blot. ( B ) The quantification of western blot gel bands in different groups (n=3 independently repeated experiments). ( C ) The gene levels of Bcl2 , Bax , and Bcl2/Bax in different groups (n=5 independently repeated experiments). ( D ) The detection of MIN6 cells apoptosis in each group using TUNEL staining. The cells with red fluorescence represent apoptosis. Scale bar 100 μm. ( E ) The quantitative analysis of TUNEL positive cells in different groups (n=5 independently repeated experiments). ( F ) The apoptosis in each group was analyzed using flow cytometry after Annexin V FITC and PI co-staining. ( G ) The quantitative analysis of flow cytometry after Annexin V FITC and PI co-staining in different groups (n=5 independently repeated experiments). Mean ± SEM. *p<0.05, **p<0.01, ***p<0.001 indicate significant differences, and ns>0.05 means no significance difference. One-way ANOVA. Figure 9—source data 1. Raw unedited gels for . Figure 9—source data 2. Uncropped and labeled gels for .

Journal: eLife

Article Title: The protective roles of eugenol on type 1 diabetes mellitus through NRF2-mediated oxidative stress pathway

doi: 10.7554/eLife.96600

Figure Lengend Snippet: ( A ) The detection of BCL2, BAX, Cleaved Caspase-3 expression in different groups using western blot. ( B ) The quantification of western blot gel bands in different groups (n=3 independently repeated experiments). ( C ) The gene levels of Bcl2 , Bax , and Bcl2/Bax in different groups (n=5 independently repeated experiments). ( D ) The detection of MIN6 cells apoptosis in each group using TUNEL staining. The cells with red fluorescence represent apoptosis. Scale bar 100 μm. ( E ) The quantitative analysis of TUNEL positive cells in different groups (n=5 independently repeated experiments). ( F ) The apoptosis in each group was analyzed using flow cytometry after Annexin V FITC and PI co-staining. ( G ) The quantitative analysis of flow cytometry after Annexin V FITC and PI co-staining in different groups (n=5 independently repeated experiments). Mean ± SEM. *p<0.05, **p<0.01, ***p<0.001 indicate significant differences, and ns>0.05 means no significance difference. One-way ANOVA. Figure 9—source data 1. Raw unedited gels for . Figure 9—source data 2. Uncropped and labeled gels for .

Article Snippet: The Annexin V FITC/PI apoptosis detection kit (Becton Dickinson, NJ, USA) was utilized for the assessment of apoptosis of MIN6 cells in different groups.

Techniques: Expressing, Western Blot, TUNEL Assay, Staining, Fluorescence, Flow Cytometry, Labeling

Journal: eLife

Article Title: The protective roles of eugenol on type 1 diabetes mellitus through NRF2-mediated oxidative stress pathway

doi: 10.7554/eLife.96600

Figure Lengend Snippet:

Article Snippet: The Annexin V FITC/PI apoptosis detection kit (Becton Dickinson, NJ, USA) was utilized for the assessment of apoptosis of MIN6 cells in different groups.

Techniques: Sequencing, Enzyme-linked Immunosorbent Assay, Glucose Assay, Protein Extraction, In Situ, Software