annexin v fitc pi apoptosis detection kit  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc annexin v fitc pi apoptosis detection kit
    Annexin V Fitc Pi Apoptosis Detection Kit, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/annexin v fitc pi apoptosis detection kit/product/Cell Signaling Technology Inc
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    annexin v fitc pi apoptosis detection kit  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc annexin v fitc pi apoptosis detection kit
    Induction of <t>apoptosis</t> in HepG2 cells by <t>annexin-V/propidium</t> iodide stain. Cytograms showing HepG2 cells treated with Pitavastatin (1.84 µM, 48 h) ( A ), HepG2 cells as an untreated control ( B ).
    Annexin V Fitc Pi Apoptosis Detection Kit, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Exploring a repurposed candidate with dual hIDO1/hTDO2 inhibitory potential for anticancer efficacy identified through pharmacophore-based virtual screening and in vitro evaluation"

    Article Title: Exploring a repurposed candidate with dual hIDO1/hTDO2 inhibitory potential for anticancer efficacy identified through pharmacophore-based virtual screening and in vitro evaluation

    Journal: Scientific Reports

    doi: 10.1038/s41598-024-59353-4

    Induction of apoptosis in HepG2 cells by annexin-V/propidium iodide stain. Cytograms showing HepG2 cells treated with Pitavastatin (1.84 µM, 48 h) ( A ), HepG2 cells as an untreated control ( B ).
    Figure Legend Snippet: Induction of apoptosis in HepG2 cells by annexin-V/propidium iodide stain. Cytograms showing HepG2 cells treated with Pitavastatin (1.84 µM, 48 h) ( A ), HepG2 cells as an untreated control ( B ).

    Techniques Used: Staining

    biomedicine pharmacotherapy 168 2023 115757 annexin v fitc pi apoptosis detection kit  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc biomedicine pharmacotherapy 168 2023 115757 annexin v fitc pi apoptosis detection kit
    Biomedicine Pharmacotherapy 168 2023 115757 Annexin V Fitc Pi Apoptosis Detection Kit, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    annexin v fitc pi apoptosis detection kit  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc annexin v fitc pi apoptosis detection kit
    Annexin V Fitc Pi Apoptosis Detection Kit, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/annexin v fitc pi apoptosis detection kit/product/Cell Signaling Technology Inc
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    pi annexin v fitc early apoptosis detection kit 6592 cell signaling  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc pi annexin v fitc early apoptosis detection kit 6592 cell signaling
    Pi Annexin V Fitc Early Apoptosis Detection Kit 6592 Cell Signaling, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    annexin v fitc propidium iodide pi apoptosis detection kit  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc annexin v fitc propidium iodide pi apoptosis detection kit
    Annexin V Fitc Propidium Iodide Pi Apoptosis Detection Kit, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/annexin v fitc propidium iodide pi apoptosis detection kit/product/Cell Signaling Technology Inc
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    annexin v fitc pi apoptosis detection kit  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc annexin v fitc pi apoptosis detection kit
    (A) In vitro cytotoxicity assay showing MTT activity across 9 days of induction in different culture conditions. Error bars represents mean ± S.D. The differences in cell proliferation between the groups were considered statistically significant at p <0.05 and p -values are indicated on the graph. (*) indicates significant increase in cell proliferation at day 9 compared to day 3 and day 6 in 5% patient sera group. (#) indicates no significant difference in hMSC proliferation at different time points across 9 days of culture. (B) Cell proliferation assay by direct cell counting reveals higher proliferation rate in 10% FBS, which was comparable to that of 10% normal sera (NS) induction group. 10% patient sera (PS) induction group did not show any increase in cell proliferation. However, 5% PS group showed increase in proliferation, which was significantly more compared to 10% PS group (* p <0.05). (C) Cell death assay by <t>annexinV-FITC/PI</t> flow cytometric quantification revealed that 10% patient sera caused mostly necrotic cell death with increase in cell death across 9 days, whereas in 5% PS group cell death was comparably minimal. However, there was negligible cell death in control groups.
    Annexin V Fitc Pi Apoptosis Detection Kit, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/annexin v fitc pi apoptosis detection kit/product/Cell Signaling Technology Inc
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    1) Product Images from "In Vitro Hepatic Trans-Differentiation of Human Mesenchymal Stem Cells Using Sera from Congestive/Ischemic Liver during Cardiac Failure"

    Article Title: In Vitro Hepatic Trans-Differentiation of Human Mesenchymal Stem Cells Using Sera from Congestive/Ischemic Liver during Cardiac Failure

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0092397

    (A) In vitro cytotoxicity assay showing MTT activity across 9 days of induction in different culture conditions. Error bars represents mean ± S.D. The differences in cell proliferation between the groups were considered statistically significant at p <0.05 and p -values are indicated on the graph. (*) indicates significant increase in cell proliferation at day 9 compared to day 3 and day 6 in 5% patient sera group. (#) indicates no significant difference in hMSC proliferation at different time points across 9 days of culture. (B) Cell proliferation assay by direct cell counting reveals higher proliferation rate in 10% FBS, which was comparable to that of 10% normal sera (NS) induction group. 10% patient sera (PS) induction group did not show any increase in cell proliferation. However, 5% PS group showed increase in proliferation, which was significantly more compared to 10% PS group (* p <0.05). (C) Cell death assay by annexinV-FITC/PI flow cytometric quantification revealed that 10% patient sera caused mostly necrotic cell death with increase in cell death across 9 days, whereas in 5% PS group cell death was comparably minimal. However, there was negligible cell death in control groups.
    Figure Legend Snippet: (A) In vitro cytotoxicity assay showing MTT activity across 9 days of induction in different culture conditions. Error bars represents mean ± S.D. The differences in cell proliferation between the groups were considered statistically significant at p <0.05 and p -values are indicated on the graph. (*) indicates significant increase in cell proliferation at day 9 compared to day 3 and day 6 in 5% patient sera group. (#) indicates no significant difference in hMSC proliferation at different time points across 9 days of culture. (B) Cell proliferation assay by direct cell counting reveals higher proliferation rate in 10% FBS, which was comparable to that of 10% normal sera (NS) induction group. 10% patient sera (PS) induction group did not show any increase in cell proliferation. However, 5% PS group showed increase in proliferation, which was significantly more compared to 10% PS group (* p <0.05). (C) Cell death assay by annexinV-FITC/PI flow cytometric quantification revealed that 10% patient sera caused mostly necrotic cell death with increase in cell death across 9 days, whereas in 5% PS group cell death was comparably minimal. However, there was negligible cell death in control groups.

    Techniques Used: In Vitro, Cytotoxicity Assay, Activity Assay, Proliferation Assay, Cell Counting

    annexin v fitc pi apoptosis detection kit  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc annexin v fitc pi apoptosis detection kit
    Annexin V Fitc Pi Apoptosis Detection Kit, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/annexin v fitc pi apoptosis detection kit/product/Cell Signaling Technology Inc
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    pi kit  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc pi kit
    Pi Kit, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pi kit/product/Cell Signaling Technology Inc
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    annexin v fitc pi apoptosis kit  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc annexin v fitc pi apoptosis kit
    (a) Dual-fluorescence flow cytometry cellometer image cytometer (K2) was used to detect the change of apoptotic rate of CNE2 cells after isoimperatorin intervention. The apoptotic rate in the 10 μ M group was 23.06 ± 2.00%, the 20 μ M group was 22.90 ± 3.47%, and the 40 μ M group was 29.06 ± 1.25% vs control group: ∗∗ P < 0.01. Cytation™ 5 cell imaging multifunctional detection system detects changes in the nucleus (b) and cell membrane potentials (c) of the cells after the intervention of isoimperatorin. (A) Control; (B)ISOIMP 10 μ M; (C) ISOIMP20 μ M; (D)ISOIMP 40 μ M; (E) CIS 4.0 μ g·mL –1 (bar=100 μ m, 200x). The nucleus appears bright blue with nuclear condensation. The increase in green fluorescence indicates a decrease in CNE2 cell membrane potentials and cell <t>apoptosis.</t> Isoimperatorin inhibits the expression of proliferation-related and apoptosis-related proteins in CNE2 cells. Western blot analysis of the effects of isoimperatorin at different concentrations on the relative expression of PCNA, XIAP, and survivin (d) and Bax and Bcl-2 (e) in CNE2 cells vs control group: ∗ P < 0.05; ∗∗ P < 0.01.
    Annexin V Fitc Pi Apoptosis Kit, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/annexin v fitc pi apoptosis kit/product/Cell Signaling Technology Inc
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    1) Product Images from "Isoimperatorin Induces Apoptosis of Nasopharyngeal Carcinoma Cells via the MAPK/ERK1/2 Signaling Pathway"

    Article Title: Isoimperatorin Induces Apoptosis of Nasopharyngeal Carcinoma Cells via the MAPK/ERK1/2 Signaling Pathway

    Journal: Evidence-based Complementary and Alternative Medicine : eCAM

    doi: 10.1155/2020/2138186

    (a) Dual-fluorescence flow cytometry cellometer image cytometer (K2) was used to detect the change of apoptotic rate of CNE2 cells after isoimperatorin intervention. The apoptotic rate in the 10 μ M group was 23.06 ± 2.00%, the 20 μ M group was 22.90 ± 3.47%, and the 40 μ M group was 29.06 ± 1.25% vs control group: ∗∗ P < 0.01. Cytation™ 5 cell imaging multifunctional detection system detects changes in the nucleus (b) and cell membrane potentials (c) of the cells after the intervention of isoimperatorin. (A) Control; (B)ISOIMP 10 μ M; (C) ISOIMP20 μ M; (D)ISOIMP 40 μ M; (E) CIS 4.0 μ g·mL –1 (bar=100 μ m, 200x). The nucleus appears bright blue with nuclear condensation. The increase in green fluorescence indicates a decrease in CNE2 cell membrane potentials and cell apoptosis. Isoimperatorin inhibits the expression of proliferation-related and apoptosis-related proteins in CNE2 cells. Western blot analysis of the effects of isoimperatorin at different concentrations on the relative expression of PCNA, XIAP, and survivin (d) and Bax and Bcl-2 (e) in CNE2 cells vs control group: ∗ P < 0.05; ∗∗ P < 0.01.
    Figure Legend Snippet: (a) Dual-fluorescence flow cytometry cellometer image cytometer (K2) was used to detect the change of apoptotic rate of CNE2 cells after isoimperatorin intervention. The apoptotic rate in the 10 μ M group was 23.06 ± 2.00%, the 20 μ M group was 22.90 ± 3.47%, and the 40 μ M group was 29.06 ± 1.25% vs control group: ∗∗ P < 0.01. Cytation™ 5 cell imaging multifunctional detection system detects changes in the nucleus (b) and cell membrane potentials (c) of the cells after the intervention of isoimperatorin. (A) Control; (B)ISOIMP 10 μ M; (C) ISOIMP20 μ M; (D)ISOIMP 40 μ M; (E) CIS 4.0 μ g·mL –1 (bar=100 μ m, 200x). The nucleus appears bright blue with nuclear condensation. The increase in green fluorescence indicates a decrease in CNE2 cell membrane potentials and cell apoptosis. Isoimperatorin inhibits the expression of proliferation-related and apoptosis-related proteins in CNE2 cells. Western blot analysis of the effects of isoimperatorin at different concentrations on the relative expression of PCNA, XIAP, and survivin (d) and Bax and Bcl-2 (e) in CNE2 cells vs control group: ∗ P < 0.05; ∗∗ P < 0.01.

    Techniques Used: Fluorescence, Flow Cytometry, Cytometry, Imaging, Expressing, Western Blot

    Effect of isoimperatorin on CNE2 cell apoptosis is attenuated by ISO. Western blot analysis shows the expression of p-c-RAF, p-MEK, and p-ERK1/2 (a), PCNA, XIAP, and survivin (b), and Bax and Bcl-2 (c) in CNE2 cells. vs control group: ∗∗ P < 0.01; vs ISOIMP group, # P < 0.05; ## P < 0.01; dual-fluorescence flow cytometry cellometer image cytometer (K2) was used to detect the change of apoptotic rate of CNE2 cells after ISO and isoimperatorin intervention (d) vs control group: ∗∗ P < 0.01; vs ISOIMP group, # P < 0.05; ## P < 0.01.
    Figure Legend Snippet: Effect of isoimperatorin on CNE2 cell apoptosis is attenuated by ISO. Western blot analysis shows the expression of p-c-RAF, p-MEK, and p-ERK1/2 (a), PCNA, XIAP, and survivin (b), and Bax and Bcl-2 (c) in CNE2 cells. vs control group: ∗∗ P < 0.01; vs ISOIMP group, # P < 0.05; ## P < 0.01; dual-fluorescence flow cytometry cellometer image cytometer (K2) was used to detect the change of apoptotic rate of CNE2 cells after ISO and isoimperatorin intervention (d) vs control group: ∗∗ P < 0.01; vs ISOIMP group, # P < 0.05; ## P < 0.01.

    Techniques Used: Western Blot, Expressing, Fluorescence, Flow Cytometry, Cytometry

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    Cell Signaling Technology Inc annexin v fitc pi apoptosis detection kit
    Annexin V Fitc Pi Apoptosis Detection Kit, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Cell Signaling Technology Inc annexin v fitc pi apoptosis kit
    (a) Dual-fluorescence flow cytometry cellometer image cytometer (K2) was used to detect the change of apoptotic rate of CNE2 cells after isoimperatorin intervention. The apoptotic rate in the 10 μ M group was 23.06 ± 2.00%, the 20 μ M group was 22.90 ± 3.47%, and the 40 μ M group was 29.06 ± 1.25% vs control group: ∗∗ P < 0.01. Cytation™ 5 cell imaging multifunctional detection system detects changes in the nucleus (b) and cell membrane potentials (c) of the cells after the intervention of isoimperatorin. (A) Control; (B)ISOIMP 10 μ M; (C) ISOIMP20 μ M; (D)ISOIMP 40 μ M; (E) CIS 4.0 μ g·mL –1 (bar=100 μ m, 200x). The nucleus appears bright blue with nuclear condensation. The increase in green fluorescence indicates a decrease in CNE2 cell membrane potentials and cell <t>apoptosis.</t> Isoimperatorin inhibits the expression of proliferation-related and apoptosis-related proteins in CNE2 cells. Western blot analysis of the effects of isoimperatorin at different concentrations on the relative expression of PCNA, XIAP, and survivin (d) and Bax and Bcl-2 (e) in CNE2 cells vs control group: ∗ P < 0.05; ∗∗ P < 0.01.
    Annexin V Fitc Pi Apoptosis Kit, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    (a) Dual-fluorescence flow cytometry cellometer image cytometer (K2) was used to detect the change of apoptotic rate of CNE2 cells after isoimperatorin intervention. The apoptotic rate in the 10 μ M group was 23.06 ± 2.00%, the 20 μ M group was 22.90 ± 3.47%, and the 40 μ M group was 29.06 ± 1.25% vs control group: ∗∗ P < 0.01. Cytation™ 5 cell imaging multifunctional detection system detects changes in the nucleus (b) and cell membrane potentials (c) of the cells after the intervention of isoimperatorin. (A) Control; (B)ISOIMP 10 μ M; (C) ISOIMP20 μ M; (D)ISOIMP 40 μ M; (E) CIS 4.0 μ g·mL –1 (bar=100 μ m, 200x). The nucleus appears bright blue with nuclear condensation. The increase in green fluorescence indicates a decrease in CNE2 cell membrane potentials and cell apoptosis. Isoimperatorin inhibits the expression of proliferation-related and apoptosis-related proteins in CNE2 cells. Western blot analysis of the effects of isoimperatorin at different concentrations on the relative expression of PCNA, XIAP, and survivin (d) and Bax and Bcl-2 (e) in CNE2 cells vs control group: ∗ P < 0.05; ∗∗ P < 0.01.

    Journal: Evidence-based Complementary and Alternative Medicine : eCAM

    Article Title: Isoimperatorin Induces Apoptosis of Nasopharyngeal Carcinoma Cells via the MAPK/ERK1/2 Signaling Pathway

    doi: 10.1155/2020/2138186

    Figure Lengend Snippet: (a) Dual-fluorescence flow cytometry cellometer image cytometer (K2) was used to detect the change of apoptotic rate of CNE2 cells after isoimperatorin intervention. The apoptotic rate in the 10 μ M group was 23.06 ± 2.00%, the 20 μ M group was 22.90 ± 3.47%, and the 40 μ M group was 29.06 ± 1.25% vs control group: ∗∗ P < 0.01. Cytation™ 5 cell imaging multifunctional detection system detects changes in the nucleus (b) and cell membrane potentials (c) of the cells after the intervention of isoimperatorin. (A) Control; (B)ISOIMP 10 μ M; (C) ISOIMP20 μ M; (D)ISOIMP 40 μ M; (E) CIS 4.0 μ g·mL –1 (bar=100 μ m, 200x). The nucleus appears bright blue with nuclear condensation. The increase in green fluorescence indicates a decrease in CNE2 cell membrane potentials and cell apoptosis. Isoimperatorin inhibits the expression of proliferation-related and apoptosis-related proteins in CNE2 cells. Western blot analysis of the effects of isoimperatorin at different concentrations on the relative expression of PCNA, XIAP, and survivin (d) and Bax and Bcl-2 (e) in CNE2 cells vs control group: ∗ P < 0.05; ∗∗ P < 0.01.

    Article Snippet: RPMI-1640 medium (Hyclone), fetal bovine serum (Gibco), DMSO (Amresco), MTT (Biosharp), Annexin V-FITC/PI apoptosis kit (MULTI SCIENCES), β -actin Mouse mAb, Survivin Rabbit mAb, XIAP Rabbit mAb, Phospho-c-Raf Rabbit mAb, Phospho-MEK Rabbit mAb, Phospho-p44/42 MAPK (ERK1/2) Rabbit mAb (Cell Signaling TECHNOLOGY), Rabbit Anti-PCNA Polyclonal Antibody, Rabbit Anti-Bax polyclonal antibody, and Rabbit Anti-Bcl-2 Polyclonal Antibody (Bioss) were used.

    Techniques: Fluorescence, Flow Cytometry, Cytometry, Imaging, Expressing, Western Blot

    Effect of isoimperatorin on CNE2 cell apoptosis is attenuated by ISO. Western blot analysis shows the expression of p-c-RAF, p-MEK, and p-ERK1/2 (a), PCNA, XIAP, and survivin (b), and Bax and Bcl-2 (c) in CNE2 cells. vs control group: ∗∗ P < 0.01; vs ISOIMP group, # P < 0.05; ## P < 0.01; dual-fluorescence flow cytometry cellometer image cytometer (K2) was used to detect the change of apoptotic rate of CNE2 cells after ISO and isoimperatorin intervention (d) vs control group: ∗∗ P < 0.01; vs ISOIMP group, # P < 0.05; ## P < 0.01.

    Journal: Evidence-based Complementary and Alternative Medicine : eCAM

    Article Title: Isoimperatorin Induces Apoptosis of Nasopharyngeal Carcinoma Cells via the MAPK/ERK1/2 Signaling Pathway

    doi: 10.1155/2020/2138186

    Figure Lengend Snippet: Effect of isoimperatorin on CNE2 cell apoptosis is attenuated by ISO. Western blot analysis shows the expression of p-c-RAF, p-MEK, and p-ERK1/2 (a), PCNA, XIAP, and survivin (b), and Bax and Bcl-2 (c) in CNE2 cells. vs control group: ∗∗ P < 0.01; vs ISOIMP group, # P < 0.05; ## P < 0.01; dual-fluorescence flow cytometry cellometer image cytometer (K2) was used to detect the change of apoptotic rate of CNE2 cells after ISO and isoimperatorin intervention (d) vs control group: ∗∗ P < 0.01; vs ISOIMP group, # P < 0.05; ## P < 0.01.

    Article Snippet: RPMI-1640 medium (Hyclone), fetal bovine serum (Gibco), DMSO (Amresco), MTT (Biosharp), Annexin V-FITC/PI apoptosis kit (MULTI SCIENCES), β -actin Mouse mAb, Survivin Rabbit mAb, XIAP Rabbit mAb, Phospho-c-Raf Rabbit mAb, Phospho-MEK Rabbit mAb, Phospho-p44/42 MAPK (ERK1/2) Rabbit mAb (Cell Signaling TECHNOLOGY), Rabbit Anti-PCNA Polyclonal Antibody, Rabbit Anti-Bax polyclonal antibody, and Rabbit Anti-Bcl-2 Polyclonal Antibody (Bioss) were used.

    Techniques: Western Blot, Expressing, Fluorescence, Flow Cytometry, Cytometry