annexin v fitc early apoptosis detection kit  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc annexin v fitc early apoptosis detection kit
    Annexin V Fitc Early Apoptosis Detection Kit, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    annexin v fitc early apoptosis detection kit  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc annexin v fitc early apoptosis detection kit
    Annexin V Fitc Early Apoptosis Detection Kit, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/annexin v fitc early apoptosis detection kit/product/Cell Signaling Technology Inc
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    annexin v fitc early apoptosis detection kit  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc annexin v fitc early apoptosis detection kit
    Annexin V Fitc Early Apoptosis Detection Kit, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/annexin v fitc early apoptosis detection kit/product/Cell Signaling Technology Inc
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    annexin v fluorescein isothiocyanate fitc early apoptosis detection kit  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc annexin v fluorescein isothiocyanate fitc early apoptosis detection kit
    Annexin V Fluorescein Isothiocyanate Fitc Early Apoptosis Detection Kit, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/annexin v fluorescein isothiocyanate fitc early apoptosis detection kit/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
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    annexin v fitc early apoptosis detection kit  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc annexin v fitc early apoptosis detection kit
    Annexin V Fitc Early Apoptosis Detection Kit, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/annexin v fitc early apoptosis detection kit/product/Cell Signaling Technology Inc
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    annexin v fitc early apoptosis detection kit  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc annexin v fitc early apoptosis detection kit
    Annexin V Fitc Early Apoptosis Detection Kit, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/annexin v fitc early apoptosis detection kit/product/Cell Signaling Technology Inc
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    annexin v fitc early apoptosis detection kit  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc annexin v fitc early apoptosis detection kit
    The silencing of FNBP1 in HeLa cells. ( A ) The expression of FNBP1 was screened at both mRNA and protein levels following silencing. Quantification of the blots were in . ( B ) The analyses of cell morphology. The yellow scale bar in the figure represented 37.5 μm (400×), and the red arrows indicated the affected cells. ( C ) The apoptotic rates were determined with PI-Annexin <t>V-FITC</t> staining by the flow cytometry (FCM) 3d after silencing. ( D ) The cell proliferation was measured using the XTT assay. ( E ) Cell cycle was analyzed with FCM 3d after silencing. The number of cells enrolled in the analyses was at least 0.5 million. ( F ) Cell adhesion activities were quantitatively evaluated by cell viability. The silencing of FNBP1 was abbreviated as si/siFNBP1. NC represented the negative control. * p < 0.05; ** p < 0.01; ns, not significant.
    Annexin V Fitc Early Apoptosis Detection Kit, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/annexin v fitc early apoptosis detection kit/product/Cell Signaling Technology Inc
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    1) Product Images from "FNBP1 Facilitates Cervical Cancer Cell Survival by the Constitutive Activation of FAK/PI3K/AKT/mTOR Signaling"

    Article Title: FNBP1 Facilitates Cervical Cancer Cell Survival by the Constitutive Activation of FAK/PI3K/AKT/mTOR Signaling

    Journal: Cells

    doi: 10.3390/cells12151964

    The silencing of FNBP1 in HeLa cells. ( A ) The expression of FNBP1 was screened at both mRNA and protein levels following silencing. Quantification of the blots were in . ( B ) The analyses of cell morphology. The yellow scale bar in the figure represented 37.5 μm (400×), and the red arrows indicated the affected cells. ( C ) The apoptotic rates were determined with PI-Annexin V-FITC staining by the flow cytometry (FCM) 3d after silencing. ( D ) The cell proliferation was measured using the XTT assay. ( E ) Cell cycle was analyzed with FCM 3d after silencing. The number of cells enrolled in the analyses was at least 0.5 million. ( F ) Cell adhesion activities were quantitatively evaluated by cell viability. The silencing of FNBP1 was abbreviated as si/siFNBP1. NC represented the negative control. * p < 0.05; ** p < 0.01; ns, not significant.
    Figure Legend Snippet: The silencing of FNBP1 in HeLa cells. ( A ) The expression of FNBP1 was screened at both mRNA and protein levels following silencing. Quantification of the blots were in . ( B ) The analyses of cell morphology. The yellow scale bar in the figure represented 37.5 μm (400×), and the red arrows indicated the affected cells. ( C ) The apoptotic rates were determined with PI-Annexin V-FITC staining by the flow cytometry (FCM) 3d after silencing. ( D ) The cell proliferation was measured using the XTT assay. ( E ) Cell cycle was analyzed with FCM 3d after silencing. The number of cells enrolled in the analyses was at least 0.5 million. ( F ) Cell adhesion activities were quantitatively evaluated by cell viability. The silencing of FNBP1 was abbreviated as si/siFNBP1. NC represented the negative control. * p < 0.05; ** p < 0.01; ns, not significant.

    Techniques Used: Expressing, Staining, Flow Cytometry, XTT Assay, Negative Control

    The inhibition of AKT activity was relieved by the specific activator. ( A ) The phosphorylation of AKT Tyr 308. Quantification of the blots were in . Analyses of cell apoptosis, proliferation, cycle and adhesion followed by the reactivation of AKT with sc79 were displayed in ( B – F ), respectively. The abbreviation of ns referred to no significant difference among the groups. The counted apoptotic cells included both the early [PI(−) Annexin V(+)] and late ones [PI(+) Annexin V(+)]. The compound of sc79 is a potent specific AKT activator. * p < 0.05; ns, not significant.
    Figure Legend Snippet: The inhibition of AKT activity was relieved by the specific activator. ( A ) The phosphorylation of AKT Tyr 308. Quantification of the blots were in . Analyses of cell apoptosis, proliferation, cycle and adhesion followed by the reactivation of AKT with sc79 were displayed in ( B – F ), respectively. The abbreviation of ns referred to no significant difference among the groups. The counted apoptotic cells included both the early [PI(−) Annexin V(+)] and late ones [PI(+) Annexin V(+)]. The compound of sc79 is a potent specific AKT activator. * p < 0.05; ns, not significant.

    Techniques Used: Inhibition, Activity Assay

    The suppression of FAK/PI3K/AKT signaling was abrogated by EGF. ( A ) The phosphorylation was rescued by the addition of EGF, which could be concomitantly negated by the selective inhibitors. Quantification of the blots are displayed in . ( B ) The cell contact area could not be rescued by the addition of EGF. The cell apoptosis, proliferation, S-phase arrest and cell adhesion restored by EGF addition has been presented in ( C – F ) individually. Before the addition of EGF, the cells were moderately harvested. The specific inhibitor of FAK (FAKI) is Y15 for blocking FAK autophosphorylation at Y397. The inhibitor of PI3K/p110 (PI3KI) is pictilisib, a potent selective inhibitor targeted to the PI3K catalytic subunit. * p < 0.05; ** p < 0.01; ns, not significant.
    Figure Legend Snippet: The suppression of FAK/PI3K/AKT signaling was abrogated by EGF. ( A ) The phosphorylation was rescued by the addition of EGF, which could be concomitantly negated by the selective inhibitors. Quantification of the blots are displayed in . ( B ) The cell contact area could not be rescued by the addition of EGF. The cell apoptosis, proliferation, S-phase arrest and cell adhesion restored by EGF addition has been presented in ( C – F ) individually. Before the addition of EGF, the cells were moderately harvested. The specific inhibitor of FAK (FAKI) is Y15 for blocking FAK autophosphorylation at Y397. The inhibitor of PI3K/p110 (PI3KI) is pictilisib, a potent selective inhibitor targeted to the PI3K catalytic subunit. * p < 0.05; ** p < 0.01; ns, not significant.

    Techniques Used: Blocking Assay

    annexin v fitc early apoptosis detection kit  (Cell Signaling Technology Inc)


    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
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    Cell Signaling Technology Inc annexin v fitc early apoptosis detection kit
    The silencing of FNBP1 in HeLa cells. ( A ) The expression of FNBP1 was screened at both mRNA and protein levels following silencing. Quantification of the blots were in . ( B ) The analyses of cell morphology. The yellow scale bar in the figure represented 37.5 μm (400×), and the red arrows indicated the affected cells. ( C ) The apoptotic rates were determined with PI-Annexin <t>V-FITC</t> staining by the flow cytometry (FCM) 3d after silencing. ( D ) The cell proliferation was measured using the XTT assay. ( E ) Cell cycle was analyzed with FCM 3d after silencing. The number of cells enrolled in the analyses was at least 0.5 million. ( F ) Cell adhesion activities were quantitatively evaluated by cell viability. The silencing of FNBP1 was abbreviated as si/siFNBP1. NC represented the negative control. * p < 0.05; ** p < 0.01; ns, not significant.
    Annexin V Fitc Early Apoptosis Detection Kit, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/annexin v fitc early apoptosis detection kit/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    annexin v fitc early apoptosis detection kit - by Bioz Stars, 2024-07
    86/100 stars

    Images

    1) Product Images from "FNBP1 Facilitates Cervical Cancer Cell Survival by the Constitutive Activation of FAK/PI3K/AKT/mTOR Signaling"

    Article Title: FNBP1 Facilitates Cervical Cancer Cell Survival by the Constitutive Activation of FAK/PI3K/AKT/mTOR Signaling

    Journal: Cells

    doi: 10.3390/cells12151964

    The silencing of FNBP1 in HeLa cells. ( A ) The expression of FNBP1 was screened at both mRNA and protein levels following silencing. Quantification of the blots were in . ( B ) The analyses of cell morphology. The yellow scale bar in the figure represented 37.5 μm (400×), and the red arrows indicated the affected cells. ( C ) The apoptotic rates were determined with PI-Annexin V-FITC staining by the flow cytometry (FCM) 3d after silencing. ( D ) The cell proliferation was measured using the XTT assay. ( E ) Cell cycle was analyzed with FCM 3d after silencing. The number of cells enrolled in the analyses was at least 0.5 million. ( F ) Cell adhesion activities were quantitatively evaluated by cell viability. The silencing of FNBP1 was abbreviated as si/siFNBP1. NC represented the negative control. * p < 0.05; ** p < 0.01; ns, not significant.
    Figure Legend Snippet: The silencing of FNBP1 in HeLa cells. ( A ) The expression of FNBP1 was screened at both mRNA and protein levels following silencing. Quantification of the blots were in . ( B ) The analyses of cell morphology. The yellow scale bar in the figure represented 37.5 μm (400×), and the red arrows indicated the affected cells. ( C ) The apoptotic rates were determined with PI-Annexin V-FITC staining by the flow cytometry (FCM) 3d after silencing. ( D ) The cell proliferation was measured using the XTT assay. ( E ) Cell cycle was analyzed with FCM 3d after silencing. The number of cells enrolled in the analyses was at least 0.5 million. ( F ) Cell adhesion activities were quantitatively evaluated by cell viability. The silencing of FNBP1 was abbreviated as si/siFNBP1. NC represented the negative control. * p < 0.05; ** p < 0.01; ns, not significant.

    Techniques Used: Expressing, Staining, Flow Cytometry, XTT Assay, Negative Control

    The inhibition of AKT activity was relieved by the specific activator. ( A ) The phosphorylation of AKT Tyr 308. Quantification of the blots were in . Analyses of cell apoptosis, proliferation, cycle and adhesion followed by the reactivation of AKT with sc79 were displayed in ( B – F ), respectively. The abbreviation of ns referred to no significant difference among the groups. The counted apoptotic cells included both the early [PI(−) Annexin V(+)] and late ones [PI(+) Annexin V(+)]. The compound of sc79 is a potent specific AKT activator. * p < 0.05; ns, not significant.
    Figure Legend Snippet: The inhibition of AKT activity was relieved by the specific activator. ( A ) The phosphorylation of AKT Tyr 308. Quantification of the blots were in . Analyses of cell apoptosis, proliferation, cycle and adhesion followed by the reactivation of AKT with sc79 were displayed in ( B – F ), respectively. The abbreviation of ns referred to no significant difference among the groups. The counted apoptotic cells included both the early [PI(−) Annexin V(+)] and late ones [PI(+) Annexin V(+)]. The compound of sc79 is a potent specific AKT activator. * p < 0.05; ns, not significant.

    Techniques Used: Inhibition, Activity Assay

    The suppression of FAK/PI3K/AKT signaling was abrogated by EGF. ( A ) The phosphorylation was rescued by the addition of EGF, which could be concomitantly negated by the selective inhibitors. Quantification of the blots are displayed in . ( B ) The cell contact area could not be rescued by the addition of EGF. The cell apoptosis, proliferation, S-phase arrest and cell adhesion restored by EGF addition has been presented in ( C – F ) individually. Before the addition of EGF, the cells were moderately harvested. The specific inhibitor of FAK (FAKI) is Y15 for blocking FAK autophosphorylation at Y397. The inhibitor of PI3K/p110 (PI3KI) is pictilisib, a potent selective inhibitor targeted to the PI3K catalytic subunit. * p < 0.05; ** p < 0.01; ns, not significant.
    Figure Legend Snippet: The suppression of FAK/PI3K/AKT signaling was abrogated by EGF. ( A ) The phosphorylation was rescued by the addition of EGF, which could be concomitantly negated by the selective inhibitors. Quantification of the blots are displayed in . ( B ) The cell contact area could not be rescued by the addition of EGF. The cell apoptosis, proliferation, S-phase arrest and cell adhesion restored by EGF addition has been presented in ( C – F ) individually. Before the addition of EGF, the cells were moderately harvested. The specific inhibitor of FAK (FAKI) is Y15 for blocking FAK autophosphorylation at Y397. The inhibitor of PI3K/p110 (PI3KI) is pictilisib, a potent selective inhibitor targeted to the PI3K catalytic subunit. * p < 0.05; ** p < 0.01; ns, not significant.

    Techniques Used: Blocking Assay

    annexin v fluorescein isothiocyanate fitc early apoptosis detection kit  (Cell Signaling Technology Inc)


    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
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    Cell Signaling Technology Inc annexin v fluorescein isothiocyanate fitc early apoptosis detection kit
    EphA4 Overexpression Aggravated Brain Damage and Neuronal <t>Apoptosis</t> after Ischemia. (A1-A5) Representative MRI images showing infarcted areas after MCAO. (A6) Infarct volume evaluated at day 3 after MCAO. (B1–B5) TUNEL (+) staining (red) plus DAPI (blue) to label apoptotic cells in the boundary zone of ischemic area. Scale Bar = 50 μm (B6) Counting of TUNEL-positive cells in peri-ischemic regions of MCAO rats on postoperative day 3. The results were represented as the mean ± SD, and the differences between groups were compared with ANOVA followed by LSD's post hoc test (n = 5, ** P < 0.01, *** P < 0.001, **** P < 0.0001, ns: no significance). (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)
    Annexin V Fluorescein Isothiocyanate Fitc Early Apoptosis Detection Kit, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/annexin v fluorescein isothiocyanate fitc early apoptosis detection kit/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
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    annexin v fluorescein isothiocyanate fitc early apoptosis detection kit - by Bioz Stars, 2024-07
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    Images

    1) Product Images from "Upregulation of EphA4 deteriorate brain damage by shifting microglia M1-polarization via NF-κB signaling after focal cerebral ischemia in rats"

    Article Title: Upregulation of EphA4 deteriorate brain damage by shifting microglia M1-polarization via NF-κB signaling after focal cerebral ischemia in rats

    Journal: Heliyon

    doi: 10.1016/j.heliyon.2023.e18429

    EphA4 Overexpression Aggravated Brain Damage and Neuronal Apoptosis after Ischemia. (A1-A5) Representative MRI images showing infarcted areas after MCAO. (A6) Infarct volume evaluated at day 3 after MCAO. (B1–B5) TUNEL (+) staining (red) plus DAPI (blue) to label apoptotic cells in the boundary zone of ischemic area. Scale Bar = 50 μm (B6) Counting of TUNEL-positive cells in peri-ischemic regions of MCAO rats on postoperative day 3. The results were represented as the mean ± SD, and the differences between groups were compared with ANOVA followed by LSD's post hoc test (n = 5, ** P < 0.01, *** P < 0.001, **** P < 0.0001, ns: no significance). (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)
    Figure Legend Snippet: EphA4 Overexpression Aggravated Brain Damage and Neuronal Apoptosis after Ischemia. (A1-A5) Representative MRI images showing infarcted areas after MCAO. (A6) Infarct volume evaluated at day 3 after MCAO. (B1–B5) TUNEL (+) staining (red) plus DAPI (blue) to label apoptotic cells in the boundary zone of ischemic area. Scale Bar = 50 μm (B6) Counting of TUNEL-positive cells in peri-ischemic regions of MCAO rats on postoperative day 3. The results were represented as the mean ± SD, and the differences between groups were compared with ANOVA followed by LSD's post hoc test (n = 5, ** P < 0.01, *** P < 0.001, **** P < 0.0001, ns: no significance). (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

    Techniques Used: Over Expression, TUNEL Assay, Staining

    EphA4 Overexpression Increased Apoptosis and Microglia Proliferation Induced by OGD. (A1-A6) Representative data of apoptosis detected by flow cytometry. (B1–B6) Microglia proliferation was tested via EdU (red) staining and DAPI staining (blue). Scale bar = 100 μm. (C–D) Summary of the percentage of apoptotic cells in each group. (E) Counting of EdU-positive microglia cells in co-cultures. The results were represented as the mean ± SD, and the differences between groups were compared with ANOVA followed by LSD's post hoc test (n = 5, * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001, ns: no significance). (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)
    Figure Legend Snippet: EphA4 Overexpression Increased Apoptosis and Microglia Proliferation Induced by OGD. (A1-A6) Representative data of apoptosis detected by flow cytometry. (B1–B6) Microglia proliferation was tested via EdU (red) staining and DAPI staining (blue). Scale bar = 100 μm. (C–D) Summary of the percentage of apoptotic cells in each group. (E) Counting of EdU-positive microglia cells in co-cultures. The results were represented as the mean ± SD, and the differences between groups were compared with ANOVA followed by LSD's post hoc test (n = 5, * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001, ns: no significance). (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

    Techniques Used: Over Expression, Flow Cytometry, Staining

    annexin v fluorescein isothiocyanate fitc early apoptosis detection kit  (Cell Signaling Technology Inc)


    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
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    Cell Signaling Technology Inc annexin v fluorescein isothiocyanate fitc early apoptosis detection kit
    Annexin V Fluorescein Isothiocyanate Fitc Early Apoptosis Detection Kit, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/annexin v fluorescein isothiocyanate fitc early apoptosis detection kit/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    annexin v fluorescein isothiocyanate fitc early apoptosis detection kit - by Bioz Stars, 2024-07
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    Cell Signaling Technology Inc annexin v fitc early apoptosis detection kit
    Annexin V Fitc Early Apoptosis Detection Kit, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/annexin v fitc early apoptosis detection kit/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
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    Cell Signaling Technology Inc annexin v fluorescein isothiocyanate fitc early apoptosis detection kit
    Annexin V Fluorescein Isothiocyanate Fitc Early Apoptosis Detection Kit, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/annexin v fluorescein isothiocyanate fitc early apoptosis detection kit/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
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    annexin v fluorescein isothiocyanate fitc early apoptosis detection kit - by Bioz Stars, 2024-07
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