Journal: International Journal of Molecular Sciences

Article Title: New 4,5-Diarylimidazol-2-ylidene–iodidogold(I) Complexes with High Activity against Esophageal Adenocarcinoma Cells

doi: 10.3390/ijms24065738

Figure Lengend Snippet: Gold complexes induced apoptosis in EAC cells. ( A – D ) Gold complexes 6b , 6d , and 6i (IC 50 concentrations) induced late-phase apoptosis in SK-GT-4 and FLO-1 cells, as assessed by an Annexin-PI assay using flow cytometry. ( E ) Cell lysates from EAC cells, when treated with complexes 6b , 6d , and 6i (IC 50 concentrations), showed significant cleavage of PARP compared to untreated controls. The treatment also reduced anti-apoptotic markers Bcl-XL, Mcl-1, and Bcl-2. * p < 0.05, ** p < 0.01.

Article Snippet: After 72 h, cells were trypsinized, washed and stained using the Annexin V-FITC Early Apoptosis Detection Kit (Cell Signaling Technology#6592) following the manufacturer’s instructions, and studied by flow cytometry.

Techniques: Flow Cytometry

Journal: Journal of Oncology

Article Title: Plumbagin Enhances the Anticancer Effects of PF Chemotherapy via Downregulation of the PI3K/AKT/mTOR/p70S6K Pathway in Human Tongue Squamous Cell Carcinoma

doi: 10.1155/2023/8306514

Figure Lengend Snippet: PB enhanced the proapoptotic effects of PF in TSCC cells. (a) Results of the flow cytometry assay demonstrated apoptosis in Cal27 and Cal27/CDDP cells following the treatment with PB and/or PF. (b-c) A histogram of the levels of apoptosis in Cal27 and Cal27/CDDP cells. (d) Representative blots of the proapoptotic protein Bax and Bad and antiapoptotic protein Bcl-2 and Bcl-xL in Cal27 and Cal27/CDDP cells after the treatment of PB and/or PF. (e) Ratio of Bax/Bcl-2 in Cal27 and Cal27/CDDP cells. (f-g) Histogram of the expression levels of Bcl-xL and Bad. Independent biological experiments were repeated three times, and statistical significance is denoted by ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001 and ∗∗∗∗ P < 0.0001. PB, plumbagin; PF, cisplatin plus 5-fluorouracil; and TSCC, tongue squamous cell carcinoma.

Article Snippet: Cell cycle kits were bought from Lianke Biological Technology Co. AnnexinV-FITC/propidium iodide apoptosis detection kits were purchased from Beibo Co. Primary antibodies against Bcl-2, Bcl-xL, Bax, Bad, PI3K, AKT, phosphorylated (p)-AKT, mTOR, p-mTOR, p70S6K, and p-p70S6K were purchased from Cell Signaling Technology (Danvers, MA, USA).

Techniques: Flow Cytometry, Expressing

Journal: Life Science Alliance

Article Title: Trans-differentiation of trophoblast stem cells: implications in placental biology

doi: 10.26508/lsa.202201583

Figure Lengend Snippet: (A, B, C) Quantitative real-time PCR (qRT) analysis of the differentially expressed endothelial cell–specific genes in trophoblast stem cells (TS) and differentiated trophoblast cells (TC). qRT–PCR results are grouped based on functional annotations. (A) Angiogenesis-related genes: Cx3cl1,c-kit , Kdr , Plau , Mmp . (B) Cell adhesion molecules: Cdh5 , Pecam1 , Itg β3, Col18a1 . (C) Apoptosis-related genes: Tnfsf10 , Bcl2 , Cradd , Casp3 . Data are representative of three biological replicates, and error bars represent SEM. * P < 0.5, ** P < 0.01. Source data are available for this figure.

Article Snippet: After 48 and 72 h, co-cultured cells were processed either for both annexin V–PI staining using early apoptosis detection kit (cat no. 6592; Cell Signaling Technology) by flow cytometry or for protein isolation and Western blotting for detection of apoptosis markers.

Techniques: Real-time Polymerase Chain Reaction, Quantitative RT-PCR, Functional Assay

Journal: PLoS ONE

Article Title: In Vitro Hepatic Trans-Differentiation of Human Mesenchymal Stem Cells Using Sera from Congestive/Ischemic Liver during Cardiac Failure

doi: 10.1371/journal.pone.0092397

Figure Lengend Snippet: (A) In vitro cytotoxicity assay showing MTT activity across 9 days of induction in different culture conditions. Error bars represents mean ± S.D. The differences in cell proliferation between the groups were considered statistically significant at p <0.05 and p -values are indicated on the graph. (*) indicates significant increase in cell proliferation at day 9 compared to day 3 and day 6 in 5% patient sera group. (#) indicates no significant difference in hMSC proliferation at different time points across 9 days of culture. (B) Cell proliferation assay by direct cell counting reveals higher proliferation rate in 10% FBS, which was comparable to that of 10% normal sera (NS) induction group. 10% patient sera (PS) induction group did not show any increase in cell proliferation. However, 5% PS group showed increase in proliferation, which was significantly more compared to 10% PS group (* p <0.05). (C) Cell death assay by annexinV-FITC/PI flow cytometric quantification revealed that 10% patient sera caused mostly necrotic cell death with increase in cell death across 9 days, whereas in 5% PS group cell death was comparably minimal. However, there was negligible cell death in control groups.

Article Snippet: Cell death assay was performed by annexin V-FITC/PI apoptosis detection kit (Cell Signaling Technology) as per manufacturer’s protocol.

Techniques: In Vitro, Cytotoxicity Assay, Activity Assay, Proliferation Assay, Cell Counting