annexin v fitc apoptosis detection kit  (Dojindo Labs)


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    Dojindo Labs annexin v fitc apoptosis detection kit
    Annexin V Fitc Apoptosis Detection Kit, supplied by Dojindo Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    annexin v fitc apoptosis detection kit  (Dojindo Labs)


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    Dojindo Labs annexin v fitc apoptosis detection kit
    <t>Apoptosis</t> analysis of RAW 264.7 cells cultured for 72 hours. (A, B) Flow cytometry showed cell apoptosis in the blank-control group and neutrophil peptide 1 group (20 µg/mL). (C, D) Statistical graphs of live cells and apoptotic cells. There was no significant difference in the number of live cells and apoptotic cells between the two groups ( P > 0.05). Data are expressed as the mean ± SD ( n = 3; one way analysis of variance followed by the least significant difference post hoc test).
    Annexin V Fitc Apoptosis Detection Kit, supplied by Dojindo Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 86 stars, based on 1 article reviews
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    1) Product Images from "Neutrophil peptide 1 accelerates the clearance of degenerative axons during Wallerian degeneration by activating macrophages after peripheral nerve crush injury"

    Article Title: Neutrophil peptide 1 accelerates the clearance of degenerative axons during Wallerian degeneration by activating macrophages after peripheral nerve crush injury

    Journal: Neural Regeneration Research

    doi: 10.4103/1673-5374.387978

    Apoptosis analysis of RAW 264.7 cells cultured for 72 hours. (A, B) Flow cytometry showed cell apoptosis in the blank-control group and neutrophil peptide 1 group (20 µg/mL). (C, D) Statistical graphs of live cells and apoptotic cells. There was no significant difference in the number of live cells and apoptotic cells between the two groups ( P > 0.05). Data are expressed as the mean ± SD ( n = 3; one way analysis of variance followed by the least significant difference post hoc test).
    Figure Legend Snippet: Apoptosis analysis of RAW 264.7 cells cultured for 72 hours. (A, B) Flow cytometry showed cell apoptosis in the blank-control group and neutrophil peptide 1 group (20 µg/mL). (C, D) Statistical graphs of live cells and apoptotic cells. There was no significant difference in the number of live cells and apoptotic cells between the two groups ( P > 0.05). Data are expressed as the mean ± SD ( n = 3; one way analysis of variance followed by the least significant difference post hoc test).

    Techniques Used: Cell Culture, Flow Cytometry

    annexin v fitc apoptosis detection kit  (Dojindo Labs)


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    Dojindo Labs annexin v fitc apoptosis detection kit
    Annexin V Fitc Apoptosis Detection Kit, supplied by Dojindo Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    annexin v fitc pi apoptosis detection kit  (Dojindo Labs)


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    Dojindo Labs annexin v fitc pi apoptosis detection kit
    Annexin V Fitc Pi Apoptosis Detection Kit, supplied by Dojindo Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/annexin v fitc pi apoptosis detection kit/product/Dojindo Labs
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    annexin v fitc apoptosis detection kit  (Dojindo Labs)


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    Dojindo Labs annexin v fitc apoptosis detection kit
    Annexin V Fitc Apoptosis Detection Kit, supplied by Dojindo Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    annexin v fitc apoptosis detection kit  (Dojindo Labs)


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    Dojindo Labs annexin v fitc apoptosis detection kit
    In vitro anticancer effects. (A) TEM was used to detect the treatment of A549 cells: (A-1) Blank control; (A-2) MgFe 2 O 4 NPs; (A-3) MgFe 2 O 4 @ZOL NPs; (A-4) MW control; (A-5) MW + MgFe 2 O 4 , and (A-6) MW + MgFe 2 O 4 @ZOL. (Red arrow indicates the cell nucleus, blue arrow indicates the endoplasmic reticulum, black arrow indicates the mitochondria, green arrow indicates the nanoparticles, and purple arrow indicates the lipid droplets.) (B) The diagram of cancer cell <t>apoptosis</t> process. (C) Fow cytometry was conducted to show the apoptosis of A549 cells in different treatment groups. (D) The apoptosis rate in different groups.
    Annexin V Fitc Apoptosis Detection Kit, supplied by Dojindo Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    annexin v fitc apoptosis detection kit - by Bioz Stars, 2024-07
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    1) Product Images from "Targeting nanoplatform synergistic glutathione depletion-enhanced chemodynamic, microwave dynamic, and selective-microwave thermal to treat lung cancer bone metastasis"

    Article Title: Targeting nanoplatform synergistic glutathione depletion-enhanced chemodynamic, microwave dynamic, and selective-microwave thermal to treat lung cancer bone metastasis

    Journal: Bioactive Materials

    doi: 10.1016/j.bioactmat.2024.04.016

    In vitro anticancer effects. (A) TEM was used to detect the treatment of A549 cells: (A-1) Blank control; (A-2) MgFe 2 O 4 NPs; (A-3) MgFe 2 O 4 @ZOL NPs; (A-4) MW control; (A-5) MW + MgFe 2 O 4 , and (A-6) MW + MgFe 2 O 4 @ZOL. (Red arrow indicates the cell nucleus, blue arrow indicates the endoplasmic reticulum, black arrow indicates the mitochondria, green arrow indicates the nanoparticles, and purple arrow indicates the lipid droplets.) (B) The diagram of cancer cell apoptosis process. (C) Fow cytometry was conducted to show the apoptosis of A549 cells in different treatment groups. (D) The apoptosis rate in different groups.
    Figure Legend Snippet: In vitro anticancer effects. (A) TEM was used to detect the treatment of A549 cells: (A-1) Blank control; (A-2) MgFe 2 O 4 NPs; (A-3) MgFe 2 O 4 @ZOL NPs; (A-4) MW control; (A-5) MW + MgFe 2 O 4 , and (A-6) MW + MgFe 2 O 4 @ZOL. (Red arrow indicates the cell nucleus, blue arrow indicates the endoplasmic reticulum, black arrow indicates the mitochondria, green arrow indicates the nanoparticles, and purple arrow indicates the lipid droplets.) (B) The diagram of cancer cell apoptosis process. (C) Fow cytometry was conducted to show the apoptosis of A549 cells in different treatment groups. (D) The apoptosis rate in different groups.

    Techniques Used: In Vitro, Cytometry

    annexin v fitc pi apoptosis detection kit  (Dojindo Labs)


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    Dojindo Labs annexin v fitc pi apoptosis detection kit
    Cellular uptake and in vitro tumor therapy. ( a ) Uptake of 4T1 cells incubated with 50 µg/mL Fe ss <t>MOF-PEG@FITC</t> at different time points. ( b ) Cell viability of 4T1 cells after different treatments. ( c ) Cell viability of 4T1 cells cocultured with Fe ss MOF-PEG and 10 µM Ferrostatin-1 (ferroptosis inhibitor), 10 µM 3-Methyladenine (autophagy inhibitor), 20 µM Z-VAD-FMK <t>(apoptosis</t> inhibitor), and 20 µM vitamin E (antioxidant) treatments for 24 h. ( d ) Colony formation ability of 4T1 cells under different inhibitors. ( e ) The levels of GSH in the cells upon different treatments. All data were presented as mean ± standard deviation. Statistical differences were calculated using two-tailed Student’s t test. Differences were considered significant when the p-value was less than or equal to 0.05. * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001
    Annexin V Fitc Pi Apoptosis Detection Kit, supplied by Dojindo Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/annexin v fitc pi apoptosis detection kit/product/Dojindo Labs
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    annexin v fitc pi apoptosis detection kit - by Bioz Stars, 2024-07
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    1) Product Images from "Iron (II)-based metal-organic framework nanozyme for boosting tumor ferroptosis through inhibiting DNA damage repair and system Xc -"

    Article Title: Iron (II)-based metal-organic framework nanozyme for boosting tumor ferroptosis through inhibiting DNA damage repair and system Xc -

    Journal: Journal of Nanobiotechnology

    doi: 10.1186/s12951-024-02508-2

    Cellular uptake and in vitro tumor therapy. ( a ) Uptake of 4T1 cells incubated with 50 µg/mL Fe ss MOF-PEG@FITC at different time points. ( b ) Cell viability of 4T1 cells after different treatments. ( c ) Cell viability of 4T1 cells cocultured with Fe ss MOF-PEG and 10 µM Ferrostatin-1 (ferroptosis inhibitor), 10 µM 3-Methyladenine (autophagy inhibitor), 20 µM Z-VAD-FMK (apoptosis inhibitor), and 20 µM vitamin E (antioxidant) treatments for 24 h. ( d ) Colony formation ability of 4T1 cells under different inhibitors. ( e ) The levels of GSH in the cells upon different treatments. All data were presented as mean ± standard deviation. Statistical differences were calculated using two-tailed Student’s t test. Differences were considered significant when the p-value was less than or equal to 0.05. * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001
    Figure Legend Snippet: Cellular uptake and in vitro tumor therapy. ( a ) Uptake of 4T1 cells incubated with 50 µg/mL Fe ss MOF-PEG@FITC at different time points. ( b ) Cell viability of 4T1 cells after different treatments. ( c ) Cell viability of 4T1 cells cocultured with Fe ss MOF-PEG and 10 µM Ferrostatin-1 (ferroptosis inhibitor), 10 µM 3-Methyladenine (autophagy inhibitor), 20 µM Z-VAD-FMK (apoptosis inhibitor), and 20 µM vitamin E (antioxidant) treatments for 24 h. ( d ) Colony formation ability of 4T1 cells under different inhibitors. ( e ) The levels of GSH in the cells upon different treatments. All data were presented as mean ± standard deviation. Statistical differences were calculated using two-tailed Student’s t test. Differences were considered significant when the p-value was less than or equal to 0.05. * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001

    Techniques Used: In Vitro, Incubation, Standard Deviation, Two Tailed Test

    Synergistic ferrotherapy. ( a ) • OH staining by a BBoxiProbe®O26 fluorescent probe in 4T1 cells after different treatments. ( b ) Lipid peroxides imaging by a Liperfluo fluorescent probe. ( c ) Flow cytometry analysis of the LPO fluorescence intensity of 4T1 cells subjected to different treatments. ( d ) Measurement of intracellular MDA levels. ( e , f ) Images of cell comet electrophoresis assay ( e ) and percentage of fluorescence intensity (Tail DNA%) in the comet tail ( f ) in 4T1 cells after different treatments. ( g ) Western-blot analysis of the expression of γ-H2AX. ( h ) Flow cytometry analysis of the apoptosis of 4T1 cells after different treatments. ( i ) Quantitative analysis of cell cycle distribution by flow cytometer. ( j , k ) Western-blot analysis of the expression of proteins that related to ferroptosis ( j ) and ferritinophagy ( k ). (1), (2), (3), and (4) indicate the groups of control, ActD, Fe ss MOF-PEG, and Fe ss MOF/ActD-PEG, respectively. All data were presented as mean ± standard deviation. Statistical differences were calculated using two-tailed Student’s t test. Differences were considered significant when the p-value was less than or equal to 0.05. * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001
    Figure Legend Snippet: Synergistic ferrotherapy. ( a ) • OH staining by a BBoxiProbe®O26 fluorescent probe in 4T1 cells after different treatments. ( b ) Lipid peroxides imaging by a Liperfluo fluorescent probe. ( c ) Flow cytometry analysis of the LPO fluorescence intensity of 4T1 cells subjected to different treatments. ( d ) Measurement of intracellular MDA levels. ( e , f ) Images of cell comet electrophoresis assay ( e ) and percentage of fluorescence intensity (Tail DNA%) in the comet tail ( f ) in 4T1 cells after different treatments. ( g ) Western-blot analysis of the expression of γ-H2AX. ( h ) Flow cytometry analysis of the apoptosis of 4T1 cells after different treatments. ( i ) Quantitative analysis of cell cycle distribution by flow cytometer. ( j , k ) Western-blot analysis of the expression of proteins that related to ferroptosis ( j ) and ferritinophagy ( k ). (1), (2), (3), and (4) indicate the groups of control, ActD, Fe ss MOF-PEG, and Fe ss MOF/ActD-PEG, respectively. All data were presented as mean ± standard deviation. Statistical differences were calculated using two-tailed Student’s t test. Differences were considered significant when the p-value was less than or equal to 0.05. * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001

    Techniques Used: Staining, Imaging, Flow Cytometry, Fluorescence, Electrophoresis, Western Blot, Expressing, Standard Deviation, Two Tailed Test

    annexin v fitc pi apoptosis detection kit  (Dojindo Labs)


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    Dojindo Labs annexin v fitc pi apoptosis detection kit
    Hhatl deficiency exacerbates ER stress–induced <t>apoptosis.</t> A and B , HeLa cells were transfected with Flag or Flag-Hhatl and then treated with DMSO or TM ( A ) or TG ( B ) for 24 h. Cell lysates were subjected to immunoblotting, and the protein levels of cleaved-caspase 3 (C-CASP3), Bcl2, and Hhatl were examined. C – E , cells were transfected with control or Hhatl siRNAs and then treated with DMSO, TM, or TG. Cell apoptosis and viability was measured separately by Western blot ( C and D ) and cell counting kit-8 assay ( E ). F and G , cells were transfected with control or Hhatl siRNAs and then treated with DMSO, TM, or TG. Cells were fixed and successively stained with TUNEL BrightGreen apoptosis detection kit ( green ), anti-Hhatl antibody ( red ), and DNAI ( blue ) ( F ). Scale bar represents 10 μm. The TUNEL-positive cells were calculated ( G ). H and I , cells were transfected with siRNAs and treated with DMSO, TM, or TG. Cells were then stained with Annexin <t>V-FITC/PI</t> Apoptosis Detection Kit, and the proportion of apoptosis was detected by flow cytometry analysis ( H ). The apoptosis rate of cells was measured ( I ). Data are presented as means ± SD ( n = 3). One-way ANOVA, ∗ p < 0.05, ∗∗ p < 0.01. ER, endoplasmic reticulum; TG, thapsigargin; TM, tunicamycin.
    Annexin V Fitc Pi Apoptosis Detection Kit, supplied by Dojindo Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    annexin v fitc pi apoptosis detection kit - by Bioz Stars, 2024-07
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    1) Product Images from "Hhatl ameliorates endoplasmic reticulum stress through autophagy by associating with LC3"

    Article Title: Hhatl ameliorates endoplasmic reticulum stress through autophagy by associating with LC3

    Journal: The Journal of Biological Chemistry

    doi: 10.1016/j.jbc.2024.107335

    Hhatl deficiency exacerbates ER stress–induced apoptosis. A and B , HeLa cells were transfected with Flag or Flag-Hhatl and then treated with DMSO or TM ( A ) or TG ( B ) for 24 h. Cell lysates were subjected to immunoblotting, and the protein levels of cleaved-caspase 3 (C-CASP3), Bcl2, and Hhatl were examined. C – E , cells were transfected with control or Hhatl siRNAs and then treated with DMSO, TM, or TG. Cell apoptosis and viability was measured separately by Western blot ( C and D ) and cell counting kit-8 assay ( E ). F and G , cells were transfected with control or Hhatl siRNAs and then treated with DMSO, TM, or TG. Cells were fixed and successively stained with TUNEL BrightGreen apoptosis detection kit ( green ), anti-Hhatl antibody ( red ), and DNAI ( blue ) ( F ). Scale bar represents 10 μm. The TUNEL-positive cells were calculated ( G ). H and I , cells were transfected with siRNAs and treated with DMSO, TM, or TG. Cells were then stained with Annexin V-FITC/PI Apoptosis Detection Kit, and the proportion of apoptosis was detected by flow cytometry analysis ( H ). The apoptosis rate of cells was measured ( I ). Data are presented as means ± SD ( n = 3). One-way ANOVA, ∗ p < 0.05, ∗∗ p < 0.01. ER, endoplasmic reticulum; TG, thapsigargin; TM, tunicamycin.
    Figure Legend Snippet: Hhatl deficiency exacerbates ER stress–induced apoptosis. A and B , HeLa cells were transfected with Flag or Flag-Hhatl and then treated with DMSO or TM ( A ) or TG ( B ) for 24 h. Cell lysates were subjected to immunoblotting, and the protein levels of cleaved-caspase 3 (C-CASP3), Bcl2, and Hhatl were examined. C – E , cells were transfected with control or Hhatl siRNAs and then treated with DMSO, TM, or TG. Cell apoptosis and viability was measured separately by Western blot ( C and D ) and cell counting kit-8 assay ( E ). F and G , cells were transfected with control or Hhatl siRNAs and then treated with DMSO, TM, or TG. Cells were fixed and successively stained with TUNEL BrightGreen apoptosis detection kit ( green ), anti-Hhatl antibody ( red ), and DNAI ( blue ) ( F ). Scale bar represents 10 μm. The TUNEL-positive cells were calculated ( G ). H and I , cells were transfected with siRNAs and treated with DMSO, TM, or TG. Cells were then stained with Annexin V-FITC/PI Apoptosis Detection Kit, and the proportion of apoptosis was detected by flow cytometry analysis ( H ). The apoptosis rate of cells was measured ( I ). Data are presented as means ± SD ( n = 3). One-way ANOVA, ∗ p < 0.05, ∗∗ p < 0.01. ER, endoplasmic reticulum; TG, thapsigargin; TM, tunicamycin.

    Techniques Used: Transfection, Western Blot, Cell Counting, Staining, TUNEL Assay, Flow Cytometry

    The interaction between Hhatl and LC3 is required for Hhatl-mediated reduction of ER stress and apoptosis. A and B , cells were first transfected with control or Hhatl siRNAs for 48 h and then transfected with Flag-Hhatl ( A ) or Flag-ΔLIR mutant ( B ) and incubated with DMSO or TM. Cell lysates were subjected to immunoblot analysis and probed with the indicated antibodies for ER stress and apoptosis. Densitometric quantification of expression of C-CASP3 and CHOP were analyzed. C and D , cells were first transfected with control or Hhatl siRNAs for 48 h, and then transfected with Flag-Hhatl ( C ) or Flag-ΔLIR mutant ( D ) and incubated with DMSO or TG. Cell lysates were subjected to immunoblot analysis, and the protein expression of C-CASP3 and CHOP were analyzed. Data are presented as means ± SD ( n = 3). One-way ANOVA, ∗ p < 0.05. C-CASP3, cleaved-caspase 3; ER, endoplasmic reticulum; LIR, LC3-interacting region; TG, thapsigargin; TM, tunicamycin.
    Figure Legend Snippet: The interaction between Hhatl and LC3 is required for Hhatl-mediated reduction of ER stress and apoptosis. A and B , cells were first transfected with control or Hhatl siRNAs for 48 h and then transfected with Flag-Hhatl ( A ) or Flag-ΔLIR mutant ( B ) and incubated with DMSO or TM. Cell lysates were subjected to immunoblot analysis and probed with the indicated antibodies for ER stress and apoptosis. Densitometric quantification of expression of C-CASP3 and CHOP were analyzed. C and D , cells were first transfected with control or Hhatl siRNAs for 48 h, and then transfected with Flag-Hhatl ( C ) or Flag-ΔLIR mutant ( D ) and incubated with DMSO or TG. Cell lysates were subjected to immunoblot analysis, and the protein expression of C-CASP3 and CHOP were analyzed. Data are presented as means ± SD ( n = 3). One-way ANOVA, ∗ p < 0.05. C-CASP3, cleaved-caspase 3; ER, endoplasmic reticulum; LIR, LC3-interacting region; TG, thapsigargin; TM, tunicamycin.

    Techniques Used: Transfection, Mutagenesis, Incubation, Western Blot, Expressing

    Model of the role of Hhatl in ER stress. Hhatl, an endoplasmic reticulum-resident protein, is significantly downregulated in response to ER stress. Hhatl protects cells from ER stress and ER stress-induced apoptosis by promoting autophagy. Treatment with autophagy inhibitor Bafilomycin A1 abolishes the protective role of Hhatl in ER stress. Conversely, treatment with autophagy activator rapamycin eliminates the pro-apoptotic effect of Hhatl depletion upon ER stress. Moreover, Hhatl physically interacts with the autophagic protein LC3 through the LIR motif. Mutation of the LIR motif eliminates Hhatl-mediated promotion of autophagy and reduction of ER stress. ER, endoplasmic reticulum; LIR, LC3-interacting region.
    Figure Legend Snippet: Model of the role of Hhatl in ER stress. Hhatl, an endoplasmic reticulum-resident protein, is significantly downregulated in response to ER stress. Hhatl protects cells from ER stress and ER stress-induced apoptosis by promoting autophagy. Treatment with autophagy inhibitor Bafilomycin A1 abolishes the protective role of Hhatl in ER stress. Conversely, treatment with autophagy activator rapamycin eliminates the pro-apoptotic effect of Hhatl depletion upon ER stress. Moreover, Hhatl physically interacts with the autophagic protein LC3 through the LIR motif. Mutation of the LIR motif eliminates Hhatl-mediated promotion of autophagy and reduction of ER stress. ER, endoplasmic reticulum; LIR, LC3-interacting region.

    Techniques Used: Mutagenesis

    fitc annexin v apoptosis detection kit  (Dojindo Labs)


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    Dojindo Labs fitc annexin v apoptosis detection kit
    Fitc Annexin V Apoptosis Detection Kit, supplied by Dojindo Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fitc annexin v apoptosis detection kit/product/Dojindo Labs
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    annexin v fitc pi apoptosis detection kit  (Dojindo Labs)


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    Dojindo Labs annexin v fitc pi apoptosis detection kit
    Schematic illustration of preparation and mechanism of hNVs@Flu-EGCG. ( A ) EGCG-modified cell membranes-derived nanoparticles are used as vehicles for targeted delivery of Flu. ( B ) The hNVs@Flu-EGCG target tumor sites, reprogramme M2 macrophages to M1 macrophages, act on androgen receptors to inhibit tumor proliferation, promote <t>apoptosis</t> by inhibiting the NF-κB pathway, reduce PSA level in the body, improve tumor drug resistance and enhance combination therapy
    Annexin V Fitc Pi Apoptosis Detection Kit, supplied by Dojindo Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/annexin v fitc pi apoptosis detection kit/product/Dojindo Labs
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    annexin v fitc pi apoptosis detection kit - by Bioz Stars, 2024-07
    86/100 stars

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    1) Product Images from "Tea polyphenol-engineered hybrid cellular nanovesicles for cancer immunotherapy and androgen deprivation therapy"

    Article Title: Tea polyphenol-engineered hybrid cellular nanovesicles for cancer immunotherapy and androgen deprivation therapy

    Journal: Journal of Nanobiotechnology

    doi: 10.1186/s12951-024-02458-9

    Schematic illustration of preparation and mechanism of hNVs@Flu-EGCG. ( A ) EGCG-modified cell membranes-derived nanoparticles are used as vehicles for targeted delivery of Flu. ( B ) The hNVs@Flu-EGCG target tumor sites, reprogramme M2 macrophages to M1 macrophages, act on androgen receptors to inhibit tumor proliferation, promote apoptosis by inhibiting the NF-κB pathway, reduce PSA level in the body, improve tumor drug resistance and enhance combination therapy
    Figure Legend Snippet: Schematic illustration of preparation and mechanism of hNVs@Flu-EGCG. ( A ) EGCG-modified cell membranes-derived nanoparticles are used as vehicles for targeted delivery of Flu. ( B ) The hNVs@Flu-EGCG target tumor sites, reprogramme M2 macrophages to M1 macrophages, act on androgen receptors to inhibit tumor proliferation, promote apoptosis by inhibiting the NF-κB pathway, reduce PSA level in the body, improve tumor drug resistance and enhance combination therapy

    Techniques Used: Modification, Derivative Assay

    The biological effect induced by nanoparticles in vitro. ( A ) The uptake ratio of hNVs@Flu-EGCG (Flu, 20 µg/mL) in RM-1 cells was analyzed using flow cytometry ( n = 3). ( B ) Confocal fluorescence images showing cellular uptake of hNVs@Flu-EGCG in RM-1 cells in at different time points. Scale bar, 10 μm. ( C ) Photographs of RM-1 cell colonies from after various treatments. ( D ) The survival fraction of RM-1 cells in cell colony formation assay ( n = 3). ( E ) Quantification of the ratios of live cells (calcein-AM + PI − ) in live/dead cell staining assay ( n = 3). ( F ) Images of hemolysis and the rate of hemolysis following exposure to varying concentrations of hNVs@Flu-EGCG ( n = 3). ( G ) Apoptosis in cells was assessed by flow cytometry with Annexin-V FITC/PI staining after different types of treatment ( n = 3). ( H ) Western blot assays demonstrated the activation of the apoptosis pathway and suppression of the NF-κB pathway in RM-1 cells after various treatments. All data are expressed as mean ± S.D. NS: no significance, * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001
    Figure Legend Snippet: The biological effect induced by nanoparticles in vitro. ( A ) The uptake ratio of hNVs@Flu-EGCG (Flu, 20 µg/mL) in RM-1 cells was analyzed using flow cytometry ( n = 3). ( B ) Confocal fluorescence images showing cellular uptake of hNVs@Flu-EGCG in RM-1 cells in at different time points. Scale bar, 10 μm. ( C ) Photographs of RM-1 cell colonies from after various treatments. ( D ) The survival fraction of RM-1 cells in cell colony formation assay ( n = 3). ( E ) Quantification of the ratios of live cells (calcein-AM + PI − ) in live/dead cell staining assay ( n = 3). ( F ) Images of hemolysis and the rate of hemolysis following exposure to varying concentrations of hNVs@Flu-EGCG ( n = 3). ( G ) Apoptosis in cells was assessed by flow cytometry with Annexin-V FITC/PI staining after different types of treatment ( n = 3). ( H ) Western blot assays demonstrated the activation of the apoptosis pathway and suppression of the NF-κB pathway in RM-1 cells after various treatments. All data are expressed as mean ± S.D. NS: no significance, * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001

    Techniques Used: In Vitro, Flow Cytometry, Fluorescence, Colony Assay, Staining, Western Blot, Activation Assay

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