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Santa Cruz Biotechnology angiogenic inducer 61 protein
Significantly upregulated proteins in human fetal retinal pigment epithelial cells (RPE) cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) with 1% sericin compared to cells cultured in DMEM without sericin, sorted by decreasing fold change (FC). FC was calculated as follows: FC = ((mean of protein expressions in RPE cultured in DMEM with 1% sericin)/(mean of protein expressions in RPE cultured in DMEM without sericin)). N = 3. FC: Fold change.
Angiogenic Inducer 61 Protein, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Article Title: Sericin-Induced Melanogenesis in Cultured Retinal Pigment Epithelial Cells Is Associated with Elevated Levels of Hydrogen Peroxide and Inflammatory Proteins

Journal: Molecules

doi: 10.3390/molecules25194395

Significantly upregulated proteins in human fetal retinal pigment epithelial cells (RPE) cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) with 1% sericin compared to cells cultured in DMEM without sericin, sorted by decreasing fold change (FC). FC was calculated as follows: FC = ((mean of protein expressions in RPE cultured in DMEM with 1% sericin)/(mean of protein expressions in RPE cultured in DMEM without sericin)). N = 3. FC: Fold change.
Figure Legend Snippet: Significantly upregulated proteins in human fetal retinal pigment epithelial cells (RPE) cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) with 1% sericin compared to cells cultured in DMEM without sericin, sorted by decreasing fold change (FC). FC was calculated as follows: FC = ((mean of protein expressions in RPE cultured in DMEM with 1% sericin)/(mean of protein expressions in RPE cultured in DMEM without sericin)). N = 3. FC: Fold change.

Techniques Used: Cell Culture, Modification

The table shows average fold change (FC) in protein levels upon incubation of primary human fetal retinal pigment epithelial cells (RPE) in Dulbecco’s Modified Eagle’s Medium (DMEM) with 1% sericin for seven days in comparison to control (cultured in DMEM alone). FC was calculated as follows: FC = ((mean of protein expressions in RPE cultured in DMEM with 1% sericin)/(mean of protein expressions in RPE cultured in DMEM without sericin)). Data are averages from triplicates.  CYR61  = Cysteine-rich  angiogenic inducer 61 protein.
Figure Legend Snippet: The table shows average fold change (FC) in protein levels upon incubation of primary human fetal retinal pigment epithelial cells (RPE) in Dulbecco’s Modified Eagle’s Medium (DMEM) with 1% sericin for seven days in comparison to control (cultured in DMEM alone). FC was calculated as follows: FC = ((mean of protein expressions in RPE cultured in DMEM with 1% sericin)/(mean of protein expressions in RPE cultured in DMEM without sericin)). Data are averages from triplicates. CYR61 = Cysteine-rich angiogenic inducer 61 protein.

Techniques Used: Incubation, Modification, Cell Culture, Immunofluorescence, Western Blot

Whole-cell lysates of human retinal pigment epithelial cells (RPE) cultured for seven days in Dulbecco’s Modified Eagle’s Medium (DMEM) with or without 1% sericin were subjected to Western blot experiments. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was used as loading control. Fold change (FC) was calculated as follows: FC = ((mean of protein expressions in RPE cultured in DMEM with 1% sericin)/(mean of protein expressions in RPE cultured in DMEM without sericin)). ( A ) Cysteine-rich angiogenic inducer 61 (CYR61); FC: 1.05; p -value: 0.90. ( B ); Laminin subunit β-2; FC: 1.11; p -value: 0.43. ( C ) Superoxide dismutase; FC: 1.36; p -value: 0.23. ( D ) Western blot. Error bars represent standard deviation of the mean. N = 3.
Figure Legend Snippet: Whole-cell lysates of human retinal pigment epithelial cells (RPE) cultured for seven days in Dulbecco’s Modified Eagle’s Medium (DMEM) with or without 1% sericin were subjected to Western blot experiments. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was used as loading control. Fold change (FC) was calculated as follows: FC = ((mean of protein expressions in RPE cultured in DMEM with 1% sericin)/(mean of protein expressions in RPE cultured in DMEM without sericin)). ( A ) Cysteine-rich angiogenic inducer 61 (CYR61); FC: 1.05; p -value: 0.90. ( B ); Laminin subunit β-2; FC: 1.11; p -value: 0.43. ( C ) Superoxide dismutase; FC: 1.36; p -value: 0.23. ( D ) Western blot. Error bars represent standard deviation of the mean. N = 3.

Techniques Used: Cell Culture, Modification, Western Blot, Standard Deviation

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    Santa Cruz Biotechnology angiogenic inducer 61 protein
    Significantly upregulated proteins in human fetal retinal pigment epithelial cells (RPE) cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) with 1% sericin compared to cells cultured in DMEM without sericin, sorted by decreasing fold change (FC). FC was calculated as follows: FC = ((mean of protein expressions in RPE cultured in DMEM with 1% sericin)/(mean of protein expressions in RPE cultured in DMEM without sericin)). N = 3. FC: Fold change.
    Angiogenic Inducer 61 Protein, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/angiogenic inducer 61 protein/product/Santa Cruz Biotechnology
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    angiogenic inducer 61 protein - by Bioz Stars, 2023-12
    94/100 stars
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    Significantly upregulated proteins in human fetal retinal pigment epithelial cells (RPE) cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) with 1% sericin compared to cells cultured in DMEM without sericin, sorted by decreasing fold change (FC). FC was calculated as follows: FC = ((mean of protein expressions in RPE cultured in DMEM with 1% sericin)/(mean of protein expressions in RPE cultured in DMEM without sericin)). N = 3. FC: Fold change.

    Journal: Molecules

    Article Title: Sericin-Induced Melanogenesis in Cultured Retinal Pigment Epithelial Cells Is Associated with Elevated Levels of Hydrogen Peroxide and Inflammatory Proteins

    doi: 10.3390/molecules25194395

    Figure Lengend Snippet: Significantly upregulated proteins in human fetal retinal pigment epithelial cells (RPE) cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) with 1% sericin compared to cells cultured in DMEM without sericin, sorted by decreasing fold change (FC). FC was calculated as follows: FC = ((mean of protein expressions in RPE cultured in DMEM with 1% sericin)/(mean of protein expressions in RPE cultured in DMEM without sericin)). N = 3. FC: Fold change.

    Article Snippet: The blots were probed with antibodies for cysteine-rich angiogenic inducer 61 protein (CYR61; 1:100; Santa Cruz sc-374129, Santa Cruz Biotechnology, Dallas, TX, USA), laminin subunit β2 (1:500; Santa Cruz sc-133241), superoxide dismutase 1 (SOD1; 1:200; Santa Cruz sc-17767), cellular retinaldehyde-binding protein (CRALBP; 1:1000; Abcam ab-154898, Abcam, Cambridge, UK), premelanosome protein (PMEL; 1:250; Santa Cruz sc-33590), and glyceraldehyde 3-phosphate dehydrogenase (GAPDH; 1:1000; Santa Cruz sc-47724).

    Techniques: Cell Culture, Modification

    The table shows average fold change (FC) in protein levels upon incubation of primary human fetal retinal pigment epithelial cells (RPE) in Dulbecco’s Modified Eagle’s Medium (DMEM) with 1% sericin for seven days in comparison to control (cultured in DMEM alone). FC was calculated as follows: FC = ((mean of protein expressions in RPE cultured in DMEM with 1% sericin)/(mean of protein expressions in RPE cultured in DMEM without sericin)). Data are averages from triplicates.  CYR61  = Cysteine-rich  angiogenic inducer 61 protein.

    Journal: Molecules

    Article Title: Sericin-Induced Melanogenesis in Cultured Retinal Pigment Epithelial Cells Is Associated with Elevated Levels of Hydrogen Peroxide and Inflammatory Proteins

    doi: 10.3390/molecules25194395

    Figure Lengend Snippet: The table shows average fold change (FC) in protein levels upon incubation of primary human fetal retinal pigment epithelial cells (RPE) in Dulbecco’s Modified Eagle’s Medium (DMEM) with 1% sericin for seven days in comparison to control (cultured in DMEM alone). FC was calculated as follows: FC = ((mean of protein expressions in RPE cultured in DMEM with 1% sericin)/(mean of protein expressions in RPE cultured in DMEM without sericin)). Data are averages from triplicates. CYR61 = Cysteine-rich angiogenic inducer 61 protein.

    Article Snippet: The blots were probed with antibodies for cysteine-rich angiogenic inducer 61 protein (CYR61; 1:100; Santa Cruz sc-374129, Santa Cruz Biotechnology, Dallas, TX, USA), laminin subunit β2 (1:500; Santa Cruz sc-133241), superoxide dismutase 1 (SOD1; 1:200; Santa Cruz sc-17767), cellular retinaldehyde-binding protein (CRALBP; 1:1000; Abcam ab-154898, Abcam, Cambridge, UK), premelanosome protein (PMEL; 1:250; Santa Cruz sc-33590), and glyceraldehyde 3-phosphate dehydrogenase (GAPDH; 1:1000; Santa Cruz sc-47724).

    Techniques: Incubation, Modification, Cell Culture, Immunofluorescence, Western Blot

    Whole-cell lysates of human retinal pigment epithelial cells (RPE) cultured for seven days in Dulbecco’s Modified Eagle’s Medium (DMEM) with or without 1% sericin were subjected to Western blot experiments. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was used as loading control. Fold change (FC) was calculated as follows: FC = ((mean of protein expressions in RPE cultured in DMEM with 1% sericin)/(mean of protein expressions in RPE cultured in DMEM without sericin)). ( A ) Cysteine-rich angiogenic inducer 61 (CYR61); FC: 1.05; p -value: 0.90. ( B ); Laminin subunit β-2; FC: 1.11; p -value: 0.43. ( C ) Superoxide dismutase; FC: 1.36; p -value: 0.23. ( D ) Western blot. Error bars represent standard deviation of the mean. N = 3.

    Journal: Molecules

    Article Title: Sericin-Induced Melanogenesis in Cultured Retinal Pigment Epithelial Cells Is Associated with Elevated Levels of Hydrogen Peroxide and Inflammatory Proteins

    doi: 10.3390/molecules25194395

    Figure Lengend Snippet: Whole-cell lysates of human retinal pigment epithelial cells (RPE) cultured for seven days in Dulbecco’s Modified Eagle’s Medium (DMEM) with or without 1% sericin were subjected to Western blot experiments. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was used as loading control. Fold change (FC) was calculated as follows: FC = ((mean of protein expressions in RPE cultured in DMEM with 1% sericin)/(mean of protein expressions in RPE cultured in DMEM without sericin)). ( A ) Cysteine-rich angiogenic inducer 61 (CYR61); FC: 1.05; p -value: 0.90. ( B ); Laminin subunit β-2; FC: 1.11; p -value: 0.43. ( C ) Superoxide dismutase; FC: 1.36; p -value: 0.23. ( D ) Western blot. Error bars represent standard deviation of the mean. N = 3.

    Article Snippet: The blots were probed with antibodies for cysteine-rich angiogenic inducer 61 protein (CYR61; 1:100; Santa Cruz sc-374129, Santa Cruz Biotechnology, Dallas, TX, USA), laminin subunit β2 (1:500; Santa Cruz sc-133241), superoxide dismutase 1 (SOD1; 1:200; Santa Cruz sc-17767), cellular retinaldehyde-binding protein (CRALBP; 1:1000; Abcam ab-154898, Abcam, Cambridge, UK), premelanosome protein (PMEL; 1:250; Santa Cruz sc-33590), and glyceraldehyde 3-phosphate dehydrogenase (GAPDH; 1:1000; Santa Cruz sc-47724).

    Techniques: Cell Culture, Modification, Western Blot, Standard Deviation