angiogenic inducer 61 cyr61  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc angiogenic inducer 61 cyr61
    The cell function analysis are all chemically synthesized interference lncRNA and eukaryotic plasmid-mediated lncRNA transient transfection. These have limited gene silencing and overexpression efficiency and show that the correlation between LNC01508 and OC cisplatin resistance is not highly significant. Furthermore, the effect of eukaryotic plasmid-mediated lncRNA overexpression is better than the chemical synthesis of lncRNA. This suggests a stable transfection in vitro to provide knockout and overexpression effects. (A) qPCR detects a lower LINC01508 expression, and the CCK-8 assay measures the cell viability. IC 50 values in LINC01508 inhibition OV2008 cells. (B) qPCR inspects a higher LINC01508 expression, and the CCK-8 assay measures the cell viability. IC 50 values in LINC01508 overexpression C13K cells. (C and D) Colony formation assays were conducted to determine the cell proliferation ability for transfected OC cells. (E and F) GAPDH, <t>Cyr61,</t> ERCC1, and p-AKT protein levels were determined by Western blot. All experiments are repeated in triplicated, and data are shown as mean±SE. lncRNA, long non-coding RNAs; OC, ovarian cancer; qPCR, quantitative real-time polymerase chain reaction; CCK-8, cell counting kit-8; IC 50 , half maximal inhibitory concentration; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; Cyr61, cysteine-rich angiogenic inducer 61; ERCC1, excision repair cross complementing‐group 1; p-AKT, phospho-AKT; SE, standard error. * p<0.05; † p<0.01.
    Angiogenic Inducer 61 Cyr61, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/angiogenic inducer 61 cyr61/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    angiogenic inducer 61 cyr61 - by Bioz Stars, 2024-06
    86/100 stars

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    1) Product Images from "Downregulation of LINC01508 contributes to cisplatin resistance in ovarian cancer via the regulation of the Hippo-YAP pathway"

    Article Title: Downregulation of LINC01508 contributes to cisplatin resistance in ovarian cancer via the regulation of the Hippo-YAP pathway

    Journal: Journal of Gynecologic Oncology

    doi: 10.3802/jgo.2021.32.e77

    The cell function analysis are all chemically synthesized interference lncRNA and eukaryotic plasmid-mediated lncRNA transient transfection. These have limited gene silencing and overexpression efficiency and show that the correlation between LNC01508 and OC cisplatin resistance is not highly significant. Furthermore, the effect of eukaryotic plasmid-mediated lncRNA overexpression is better than the chemical synthesis of lncRNA. This suggests a stable transfection in vitro to provide knockout and overexpression effects. (A) qPCR detects a lower LINC01508 expression, and the CCK-8 assay measures the cell viability. IC 50 values in LINC01508 inhibition OV2008 cells. (B) qPCR inspects a higher LINC01508 expression, and the CCK-8 assay measures the cell viability. IC 50 values in LINC01508 overexpression C13K cells. (C and D) Colony formation assays were conducted to determine the cell proliferation ability for transfected OC cells. (E and F) GAPDH, Cyr61, ERCC1, and p-AKT protein levels were determined by Western blot. All experiments are repeated in triplicated, and data are shown as mean±SE. lncRNA, long non-coding RNAs; OC, ovarian cancer; qPCR, quantitative real-time polymerase chain reaction; CCK-8, cell counting kit-8; IC 50 , half maximal inhibitory concentration; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; Cyr61, cysteine-rich angiogenic inducer 61; ERCC1, excision repair cross complementing‐group 1; p-AKT, phospho-AKT; SE, standard error. * p<0.05; † p<0.01.
    Figure Legend Snippet: The cell function analysis are all chemically synthesized interference lncRNA and eukaryotic plasmid-mediated lncRNA transient transfection. These have limited gene silencing and overexpression efficiency and show that the correlation between LNC01508 and OC cisplatin resistance is not highly significant. Furthermore, the effect of eukaryotic plasmid-mediated lncRNA overexpression is better than the chemical synthesis of lncRNA. This suggests a stable transfection in vitro to provide knockout and overexpression effects. (A) qPCR detects a lower LINC01508 expression, and the CCK-8 assay measures the cell viability. IC 50 values in LINC01508 inhibition OV2008 cells. (B) qPCR inspects a higher LINC01508 expression, and the CCK-8 assay measures the cell viability. IC 50 values in LINC01508 overexpression C13K cells. (C and D) Colony formation assays were conducted to determine the cell proliferation ability for transfected OC cells. (E and F) GAPDH, Cyr61, ERCC1, and p-AKT protein levels were determined by Western blot. All experiments are repeated in triplicated, and data are shown as mean±SE. lncRNA, long non-coding RNAs; OC, ovarian cancer; qPCR, quantitative real-time polymerase chain reaction; CCK-8, cell counting kit-8; IC 50 , half maximal inhibitory concentration; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; Cyr61, cysteine-rich angiogenic inducer 61; ERCC1, excision repair cross complementing‐group 1; p-AKT, phospho-AKT; SE, standard error. * p<0.05; † p<0.01.

    Techniques Used: Cell Function Assay, Synthesized, Plasmid Preparation, Transfection, Over Expression, Stable Transfection, In Vitro, Knock-Out, Expressing, CCK-8 Assay, Inhibition, Western Blot, Real-time Polymerase Chain Reaction, Cell Counting, Concentration Assay

    (A) Western blot analyses of YAP and p-YAP protein expression in OC cells. (B and C) Cell survival rate and colony formation pictures of C13K cells after treatment of verteporfin and different doses of cisplatin. Data were shown as mean±SE of 3 experiments. (D) Cyr61 protein is marked by green fluorescence. Cyr61 protein was mainly localized in the cytoplasm. Nuclear staining with DAPI is shown in blue (magnification ×400). Representative images from 3 independent experiments are shown. OC, ovarian cancer; YAP, yes-associated protein 1; p-YAP, phospho-YAP; SE, standard error; Cyr61, cysteine-rich angiogenic inducer 61; DAPI, diamidino-2-phenylindole; DMSO, dimethyl sulfoxide. * p<0.05; † p<0.01, compared with DMSO group.
    Figure Legend Snippet: (A) Western blot analyses of YAP and p-YAP protein expression in OC cells. (B and C) Cell survival rate and colony formation pictures of C13K cells after treatment of verteporfin and different doses of cisplatin. Data were shown as mean±SE of 3 experiments. (D) Cyr61 protein is marked by green fluorescence. Cyr61 protein was mainly localized in the cytoplasm. Nuclear staining with DAPI is shown in blue (magnification ×400). Representative images from 3 independent experiments are shown. OC, ovarian cancer; YAP, yes-associated protein 1; p-YAP, phospho-YAP; SE, standard error; Cyr61, cysteine-rich angiogenic inducer 61; DAPI, diamidino-2-phenylindole; DMSO, dimethyl sulfoxide. * p<0.05; † p<0.01, compared with DMSO group.

    Techniques Used: Western Blot, Expressing, Fluorescence, Staining

    angiogenic inducer 61 cyr61  (Cell Signaling Technology Inc)


    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
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    Cell Signaling Technology Inc angiogenic inducer 61 cyr61
    Catechol inhibits YAP expression through AMPK activation in Panc-1 cells. Results of western blot analysis of AMPK and YAP signaling pathway proteins after exposure to catechol for 48 h. GAPDH was used as the loading control. Band intensities were measured using ImageJ software (version 1.48). Values represent the means ± SD (n=3). *P<0.05 vs. the control. YAP, yes-associated protein; AMPK, AMP-activated protein kinase; p-, phosphorylated; <t>CYR61,</t> cysteine-rich angiogenic inducer 61; CTGF, connective tissue growth factor; SD, standard deviation.
    Angiogenic Inducer 61 Cyr61, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/angiogenic inducer 61 cyr61/product/Cell Signaling Technology Inc
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    angiogenic inducer 61 cyr61 - by Bioz Stars, 2024-06
    94/100 stars

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    1) Product Images from "Catechol enhances chemo- and radio-sensitivity by targeting AMPK/Hippo signaling in pancreatic cancer cells"

    Article Title: Catechol enhances chemo- and radio-sensitivity by targeting AMPK/Hippo signaling in pancreatic cancer cells

    Journal: Oncology Reports

    doi: 10.3892/or.2021.7924

    Catechol inhibits YAP expression through AMPK activation in Panc-1 cells. Results of western blot analysis of AMPK and YAP signaling pathway proteins after exposure to catechol for 48 h. GAPDH was used as the loading control. Band intensities were measured using ImageJ software (version 1.48). Values represent the means ± SD (n=3). *P<0.05 vs. the control. YAP, yes-associated protein; AMPK, AMP-activated protein kinase; p-, phosphorylated; CYR61, cysteine-rich angiogenic inducer 61; CTGF, connective tissue growth factor; SD, standard deviation.
    Figure Legend Snippet: Catechol inhibits YAP expression through AMPK activation in Panc-1 cells. Results of western blot analysis of AMPK and YAP signaling pathway proteins after exposure to catechol for 48 h. GAPDH was used as the loading control. Band intensities were measured using ImageJ software (version 1.48). Values represent the means ± SD (n=3). *P<0.05 vs. the control. YAP, yes-associated protein; AMPK, AMP-activated protein kinase; p-, phosphorylated; CYR61, cysteine-rich angiogenic inducer 61; CTGF, connective tissue growth factor; SD, standard deviation.

    Techniques Used: Expressing, Activation Assay, Western Blot, Software, Standard Deviation

    Catechol enhances the chemosensitivity of Panc-1 cells to gemcitabine. (A) Synergistic anti-proliferative effects of catechol and gemcitabine in Panc-1 cells as measured by the MTT assay. (B) CI values calculated for treatments exposing Panc-1 cells to combinations of various concentrations of catechol and gemcitabine. (C and D) Following exposure to 50 µM of catechol, 0.25 µM of gemcitabine, 0.5 µM of gemcitabine, or catechol plus gemcitabine for 48 h, Hoechst 33342 staining and cell cycle analysis were carried out. (E) Protein levels were examined using western blot analysis after exposing Panc-1 cells to catechol. GAPDH was used as the loading control. Band intensities were measured using ImageJ software (version 1.48). Values represent the means ± SDs (n=3). *P<0.05 vs. the control. CT, catechol; GEM, gemcitabine; CI, combination index; SDs, standard deviations; AMPK, AMP-activated protein kinase; p-, phosphorylated; YAP, yes-associated protein; CYR61, cysteine-rich angiogenic inducer 61; CTGF, connective tissue growth factor; PARP, poly(ADP-ribose) polymerase.
    Figure Legend Snippet: Catechol enhances the chemosensitivity of Panc-1 cells to gemcitabine. (A) Synergistic anti-proliferative effects of catechol and gemcitabine in Panc-1 cells as measured by the MTT assay. (B) CI values calculated for treatments exposing Panc-1 cells to combinations of various concentrations of catechol and gemcitabine. (C and D) Following exposure to 50 µM of catechol, 0.25 µM of gemcitabine, 0.5 µM of gemcitabine, or catechol plus gemcitabine for 48 h, Hoechst 33342 staining and cell cycle analysis were carried out. (E) Protein levels were examined using western blot analysis after exposing Panc-1 cells to catechol. GAPDH was used as the loading control. Band intensities were measured using ImageJ software (version 1.48). Values represent the means ± SDs (n=3). *P<0.05 vs. the control. CT, catechol; GEM, gemcitabine; CI, combination index; SDs, standard deviations; AMPK, AMP-activated protein kinase; p-, phosphorylated; YAP, yes-associated protein; CYR61, cysteine-rich angiogenic inducer 61; CTGF, connective tissue growth factor; PARP, poly(ADP-ribose) polymerase.

    Techniques Used: MTT Assay, Staining, Cell Cycle Assay, Western Blot, Software

    Catechol enhances the radiosensitivity of Panc-1 cells. (A) Clonogenic assay after treatment with catechol (CT; 50 µM) and radiation (IR; 2 or 4 Gy). (B) The results obtained from the clonogenic assay. (C) Cell cycle analysis of Panc-1 cells following treatment with (50 µM) or without CT and IR (4 Gy). (D) Protein levels in Panc-1 cells were examined using western blot analysis with GAPDH as the loading control. Band intensities were measured using ImageJ software (version 1.48). Values represent the means ± SDs (n=3). *P<0.05 vs. the control. CT, catechol; IR, radiation; SDs, standard deviations; AMPK, AMP-activated protein kinase; p-, phosphorylated; YAP, yes-associated protein; CYR61, cysteine-rich angiogenic inducer 61; CTGF, connective tissue growth factor; PARP, poly(ADP-ribose) polymerase.
    Figure Legend Snippet: Catechol enhances the radiosensitivity of Panc-1 cells. (A) Clonogenic assay after treatment with catechol (CT; 50 µM) and radiation (IR; 2 or 4 Gy). (B) The results obtained from the clonogenic assay. (C) Cell cycle analysis of Panc-1 cells following treatment with (50 µM) or without CT and IR (4 Gy). (D) Protein levels in Panc-1 cells were examined using western blot analysis with GAPDH as the loading control. Band intensities were measured using ImageJ software (version 1.48). Values represent the means ± SDs (n=3). *P<0.05 vs. the control. CT, catechol; IR, radiation; SDs, standard deviations; AMPK, AMP-activated protein kinase; p-, phosphorylated; YAP, yes-associated protein; CYR61, cysteine-rich angiogenic inducer 61; CTGF, connective tissue growth factor; PARP, poly(ADP-ribose) polymerase.

    Techniques Used: Clonogenic Assay, Cell Cycle Assay, Western Blot, Software

    angiogenic inducer 61 cyr61  (Cell Signaling Technology Inc)


    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
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    Cell Signaling Technology Inc angiogenic inducer 61 cyr61
    Angiogenic Inducer 61 Cyr61, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/angiogenic inducer 61 cyr61/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    angiogenic inducer 61 cyr61 - by Bioz Stars, 2024-06
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    angiogenic inducer 61 cyr61  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc angiogenic inducer 61 cyr61
    Angiogenic Inducer 61 Cyr61, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/angiogenic inducer 61 cyr61/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
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    cysteine rich angiogenic inducer 61  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc cysteine rich angiogenic inducer 61
    Sequences of primers for reverse transcription-quantitative polymerase chain reaction.
    Cysteine Rich Angiogenic Inducer 61, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cysteine rich angiogenic inducer 61/product/Cell Signaling Technology Inc
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    1) Product Images from "Loss of miR-873 contributes to gemcitabine resistance in triple-negative breast cancer via targeting ZEB1"

    Article Title: Loss of miR-873 contributes to gemcitabine resistance in triple-negative breast cancer via targeting ZEB1

    Journal: Oncology Letters

    doi: 10.3892/ol.2019.10697

    Sequences of primers for reverse transcription-quantitative polymerase chain reaction.
    Figure Legend Snippet: Sequences of primers for reverse transcription-quantitative polymerase chain reaction.

    Techniques Used: Sequencing

    miR-873 represses ZEB1 expression in triple-negative breast cancer cells. (A) Overexpression of miR-873 decreased ZEB1 mRNA level in MDA-MB-231 and BT549 cells. Overexpression of miR-873 decreased ZEB1 protein level and elevated E-cadherin protein level in (B) MDA-MB-231 and (C) BT549 cells. Overexpression of miR-873 decreased AXL, CTGF and CYR61 mRNA levels in (D) MDA-MB-231 and (E) BT549 cells. Overexpression of miR-873 decreased AXL, CTGF and CYR61 protein levels in (F) MDA-MB-231 and (G) BT549 cells. *P<0.05, **P<0.01 and ***P<0.0001 vs. miR-NC mimics. ZEB1, zinc finger E-box binding homeobox 1; NC, negative control; AXL, AXL receptor tyrosine kinase; CTGF, connective tissue growth factor; CYR61, cysteine rich angiogenic inducer 61; miR, microRNA.
    Figure Legend Snippet: miR-873 represses ZEB1 expression in triple-negative breast cancer cells. (A) Overexpression of miR-873 decreased ZEB1 mRNA level in MDA-MB-231 and BT549 cells. Overexpression of miR-873 decreased ZEB1 protein level and elevated E-cadherin protein level in (B) MDA-MB-231 and (C) BT549 cells. Overexpression of miR-873 decreased AXL, CTGF and CYR61 mRNA levels in (D) MDA-MB-231 and (E) BT549 cells. Overexpression of miR-873 decreased AXL, CTGF and CYR61 protein levels in (F) MDA-MB-231 and (G) BT549 cells. *P<0.05, **P<0.01 and ***P<0.0001 vs. miR-NC mimics. ZEB1, zinc finger E-box binding homeobox 1; NC, negative control; AXL, AXL receptor tyrosine kinase; CTGF, connective tissue growth factor; CYR61, cysteine rich angiogenic inducer 61; miR, microRNA.

    Techniques Used: Expressing, Over Expression, Binding Assay, Negative Control

    cysteine rich angiogenic inducer 61  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc cysteine rich angiogenic inducer 61
    Sequences of primers for reverse transcription-quantitative polymerase chain reaction.
    Cysteine Rich Angiogenic Inducer 61, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cysteine rich angiogenic inducer 61/product/Cell Signaling Technology Inc
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cysteine rich angiogenic inducer 61 - by Bioz Stars, 2024-06
    94/100 stars

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    1) Product Images from "Loss of miR-873 contributes to gemcitabine resistance in triple-negative breast cancer via targeting ZEB1"

    Article Title: Loss of miR-873 contributes to gemcitabine resistance in triple-negative breast cancer via targeting ZEB1

    Journal: Oncology Letters

    doi: 10.3892/ol.2019.10697

    Sequences of primers for reverse transcription-quantitative polymerase chain reaction.
    Figure Legend Snippet: Sequences of primers for reverse transcription-quantitative polymerase chain reaction.

    Techniques Used: Sequencing

    miR-873 represses ZEB1 expression in triple-negative breast cancer cells. (A) Overexpression of miR-873 decreased ZEB1 mRNA level in MDA-MB-231 and BT549 cells. Overexpression of miR-873 decreased ZEB1 protein level and elevated E-cadherin protein level in (B) MDA-MB-231 and (C) BT549 cells. Overexpression of miR-873 decreased AXL, CTGF and CYR61 mRNA levels in (D) MDA-MB-231 and (E) BT549 cells. Overexpression of miR-873 decreased AXL, CTGF and CYR61 protein levels in (F) MDA-MB-231 and (G) BT549 cells. *P<0.05, **P<0.01 and ***P<0.0001 vs. miR-NC mimics. ZEB1, zinc finger E-box binding homeobox 1; NC, negative control; AXL, AXL receptor tyrosine kinase; CTGF, connective tissue growth factor; CYR61, cysteine rich angiogenic inducer 61; miR, microRNA.
    Figure Legend Snippet: miR-873 represses ZEB1 expression in triple-negative breast cancer cells. (A) Overexpression of miR-873 decreased ZEB1 mRNA level in MDA-MB-231 and BT549 cells. Overexpression of miR-873 decreased ZEB1 protein level and elevated E-cadherin protein level in (B) MDA-MB-231 and (C) BT549 cells. Overexpression of miR-873 decreased AXL, CTGF and CYR61 mRNA levels in (D) MDA-MB-231 and (E) BT549 cells. Overexpression of miR-873 decreased AXL, CTGF and CYR61 protein levels in (F) MDA-MB-231 and (G) BT549 cells. *P<0.05, **P<0.01 and ***P<0.0001 vs. miR-NC mimics. ZEB1, zinc finger E-box binding homeobox 1; NC, negative control; AXL, AXL receptor tyrosine kinase; CTGF, connective tissue growth factor; CYR61, cysteine rich angiogenic inducer 61; miR, microRNA.

    Techniques Used: Expressing, Over Expression, Binding Assay, Negative Control

    cysteine rich angiogenic inducer 61  (Cell Signaling Technology Inc)


    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
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    Cell Signaling Technology Inc cysteine rich angiogenic inducer 61
    Sequences of primers for reverse transcription-quantitative polymerase chain reaction.
    Cysteine Rich Angiogenic Inducer 61, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cysteine rich angiogenic inducer 61/product/Cell Signaling Technology Inc
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cysteine rich angiogenic inducer 61 - by Bioz Stars, 2024-06
    94/100 stars

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    1) Product Images from "Loss of miR-873 contributes to gemcitabine resistance in triple-negative breast cancer via targeting ZEB1"

    Article Title: Loss of miR-873 contributes to gemcitabine resistance in triple-negative breast cancer via targeting ZEB1

    Journal: Oncology Letters

    doi: 10.3892/ol.2019.10697

    Sequences of primers for reverse transcription-quantitative polymerase chain reaction.
    Figure Legend Snippet: Sequences of primers for reverse transcription-quantitative polymerase chain reaction.

    Techniques Used: Sequencing

    miR-873 represses ZEB1 expression in triple-negative breast cancer cells. (A) Overexpression of miR-873 decreased ZEB1 mRNA level in MDA-MB-231 and BT549 cells. Overexpression of miR-873 decreased ZEB1 protein level and elevated E-cadherin protein level in (B) MDA-MB-231 and (C) BT549 cells. Overexpression of miR-873 decreased AXL, CTGF and CYR61 mRNA levels in (D) MDA-MB-231 and (E) BT549 cells. Overexpression of miR-873 decreased AXL, CTGF and CYR61 protein levels in (F) MDA-MB-231 and (G) BT549 cells. *P<0.05, **P<0.01 and ***P<0.0001 vs. miR-NC mimics. ZEB1, zinc finger E-box binding homeobox 1; NC, negative control; AXL, AXL receptor tyrosine kinase; CTGF, connective tissue growth factor; CYR61, cysteine rich angiogenic inducer 61; miR, microRNA.
    Figure Legend Snippet: miR-873 represses ZEB1 expression in triple-negative breast cancer cells. (A) Overexpression of miR-873 decreased ZEB1 mRNA level in MDA-MB-231 and BT549 cells. Overexpression of miR-873 decreased ZEB1 protein level and elevated E-cadherin protein level in (B) MDA-MB-231 and (C) BT549 cells. Overexpression of miR-873 decreased AXL, CTGF and CYR61 mRNA levels in (D) MDA-MB-231 and (E) BT549 cells. Overexpression of miR-873 decreased AXL, CTGF and CYR61 protein levels in (F) MDA-MB-231 and (G) BT549 cells. *P<0.05, **P<0.01 and ***P<0.0001 vs. miR-NC mimics. ZEB1, zinc finger E-box binding homeobox 1; NC, negative control; AXL, AXL receptor tyrosine kinase; CTGF, connective tissue growth factor; CYR61, cysteine rich angiogenic inducer 61; miR, microRNA.

    Techniques Used: Expressing, Over Expression, Binding Assay, Negative Control

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    Cell Signaling Technology Inc angiogenic inducer 61 cyr61
    The cell function analysis are all chemically synthesized interference lncRNA and eukaryotic plasmid-mediated lncRNA transient transfection. These have limited gene silencing and overexpression efficiency and show that the correlation between LNC01508 and OC cisplatin resistance is not highly significant. Furthermore, the effect of eukaryotic plasmid-mediated lncRNA overexpression is better than the chemical synthesis of lncRNA. This suggests a stable transfection in vitro to provide knockout and overexpression effects. (A) qPCR detects a lower LINC01508 expression, and the CCK-8 assay measures the cell viability. IC 50 values in LINC01508 inhibition OV2008 cells. (B) qPCR inspects a higher LINC01508 expression, and the CCK-8 assay measures the cell viability. IC 50 values in LINC01508 overexpression C13K cells. (C and D) Colony formation assays were conducted to determine the cell proliferation ability for transfected OC cells. (E and F) GAPDH, <t>Cyr61,</t> ERCC1, and p-AKT protein levels were determined by Western blot. All experiments are repeated in triplicated, and data are shown as mean±SE. lncRNA, long non-coding RNAs; OC, ovarian cancer; qPCR, quantitative real-time polymerase chain reaction; CCK-8, cell counting kit-8; IC 50 , half maximal inhibitory concentration; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; Cyr61, cysteine-rich angiogenic inducer 61; ERCC1, excision repair cross complementing‐group 1; p-AKT, phospho-AKT; SE, standard error. * p<0.05; † p<0.01.
    Angiogenic Inducer 61 Cyr61, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/angiogenic inducer 61 cyr61/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    angiogenic inducer 61 cyr61 - by Bioz Stars, 2024-06
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    Cell Signaling Technology Inc cysteine rich angiogenic inducer 61
    Sequences of primers for reverse transcription-quantitative polymerase chain reaction.
    Cysteine Rich Angiogenic Inducer 61, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cysteine rich angiogenic inducer 61/product/Cell Signaling Technology Inc
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cysteine rich angiogenic inducer 61 - by Bioz Stars, 2024-06
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    The cell function analysis are all chemically synthesized interference lncRNA and eukaryotic plasmid-mediated lncRNA transient transfection. These have limited gene silencing and overexpression efficiency and show that the correlation between LNC01508 and OC cisplatin resistance is not highly significant. Furthermore, the effect of eukaryotic plasmid-mediated lncRNA overexpression is better than the chemical synthesis of lncRNA. This suggests a stable transfection in vitro to provide knockout and overexpression effects. (A) qPCR detects a lower LINC01508 expression, and the CCK-8 assay measures the cell viability. IC 50 values in LINC01508 inhibition OV2008 cells. (B) qPCR inspects a higher LINC01508 expression, and the CCK-8 assay measures the cell viability. IC 50 values in LINC01508 overexpression C13K cells. (C and D) Colony formation assays were conducted to determine the cell proliferation ability for transfected OC cells. (E and F) GAPDH, Cyr61, ERCC1, and p-AKT protein levels were determined by Western blot. All experiments are repeated in triplicated, and data are shown as mean±SE. lncRNA, long non-coding RNAs; OC, ovarian cancer; qPCR, quantitative real-time polymerase chain reaction; CCK-8, cell counting kit-8; IC 50 , half maximal inhibitory concentration; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; Cyr61, cysteine-rich angiogenic inducer 61; ERCC1, excision repair cross complementing‐group 1; p-AKT, phospho-AKT; SE, standard error. * p<0.05; † p<0.01.

    Journal: Journal of Gynecologic Oncology

    Article Title: Downregulation of LINC01508 contributes to cisplatin resistance in ovarian cancer via the regulation of the Hippo-YAP pathway

    doi: 10.3802/jgo.2021.32.e77

    Figure Lengend Snippet: The cell function analysis are all chemically synthesized interference lncRNA and eukaryotic plasmid-mediated lncRNA transient transfection. These have limited gene silencing and overexpression efficiency and show that the correlation between LNC01508 and OC cisplatin resistance is not highly significant. Furthermore, the effect of eukaryotic plasmid-mediated lncRNA overexpression is better than the chemical synthesis of lncRNA. This suggests a stable transfection in vitro to provide knockout and overexpression effects. (A) qPCR detects a lower LINC01508 expression, and the CCK-8 assay measures the cell viability. IC 50 values in LINC01508 inhibition OV2008 cells. (B) qPCR inspects a higher LINC01508 expression, and the CCK-8 assay measures the cell viability. IC 50 values in LINC01508 overexpression C13K cells. (C and D) Colony formation assays were conducted to determine the cell proliferation ability for transfected OC cells. (E and F) GAPDH, Cyr61, ERCC1, and p-AKT protein levels were determined by Western blot. All experiments are repeated in triplicated, and data are shown as mean±SE. lncRNA, long non-coding RNAs; OC, ovarian cancer; qPCR, quantitative real-time polymerase chain reaction; CCK-8, cell counting kit-8; IC 50 , half maximal inhibitory concentration; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; Cyr61, cysteine-rich angiogenic inducer 61; ERCC1, excision repair cross complementing‐group 1; p-AKT, phospho-AKT; SE, standard error. * p<0.05; † p<0.01.

    Article Snippet: The primary antibodies used were as follows: yes-associated protein (YAP) and cysteine-rich angiogenic inducer 61 (Cyr61) (sc-15407 and sc-13100, respectively; Santa Cruz Biotechnology, Dallas, TX, USA), GAPDH and phospho-AKT (p-AKT) (sc-166574 and sc-135650, respectively; Santa Cruz Biotechnology), phospho-YAP (p-YAP) and excision repair cross complementing‐group 1 (ERCC1) (4911 and 3885s, respectively; Cell Signaling Technology).

    Techniques: Cell Function Assay, Synthesized, Plasmid Preparation, Transfection, Over Expression, Stable Transfection, In Vitro, Knock-Out, Expressing, CCK-8 Assay, Inhibition, Western Blot, Real-time Polymerase Chain Reaction, Cell Counting, Concentration Assay

    (A) Western blot analyses of YAP and p-YAP protein expression in OC cells. (B and C) Cell survival rate and colony formation pictures of C13K cells after treatment of verteporfin and different doses of cisplatin. Data were shown as mean±SE of 3 experiments. (D) Cyr61 protein is marked by green fluorescence. Cyr61 protein was mainly localized in the cytoplasm. Nuclear staining with DAPI is shown in blue (magnification ×400). Representative images from 3 independent experiments are shown. OC, ovarian cancer; YAP, yes-associated protein 1; p-YAP, phospho-YAP; SE, standard error; Cyr61, cysteine-rich angiogenic inducer 61; DAPI, diamidino-2-phenylindole; DMSO, dimethyl sulfoxide. * p<0.05; † p<0.01, compared with DMSO group.

    Journal: Journal of Gynecologic Oncology

    Article Title: Downregulation of LINC01508 contributes to cisplatin resistance in ovarian cancer via the regulation of the Hippo-YAP pathway

    doi: 10.3802/jgo.2021.32.e77

    Figure Lengend Snippet: (A) Western blot analyses of YAP and p-YAP protein expression in OC cells. (B and C) Cell survival rate and colony formation pictures of C13K cells after treatment of verteporfin and different doses of cisplatin. Data were shown as mean±SE of 3 experiments. (D) Cyr61 protein is marked by green fluorescence. Cyr61 protein was mainly localized in the cytoplasm. Nuclear staining with DAPI is shown in blue (magnification ×400). Representative images from 3 independent experiments are shown. OC, ovarian cancer; YAP, yes-associated protein 1; p-YAP, phospho-YAP; SE, standard error; Cyr61, cysteine-rich angiogenic inducer 61; DAPI, diamidino-2-phenylindole; DMSO, dimethyl sulfoxide. * p<0.05; † p<0.01, compared with DMSO group.

    Article Snippet: The primary antibodies used were as follows: yes-associated protein (YAP) and cysteine-rich angiogenic inducer 61 (Cyr61) (sc-15407 and sc-13100, respectively; Santa Cruz Biotechnology, Dallas, TX, USA), GAPDH and phospho-AKT (p-AKT) (sc-166574 and sc-135650, respectively; Santa Cruz Biotechnology), phospho-YAP (p-YAP) and excision repair cross complementing‐group 1 (ERCC1) (4911 and 3885s, respectively; Cell Signaling Technology).

    Techniques: Western Blot, Expressing, Fluorescence, Staining

    Sequences of primers for reverse transcription-quantitative polymerase chain reaction.

    Journal: Oncology Letters

    Article Title: Loss of miR-873 contributes to gemcitabine resistance in triple-negative breast cancer via targeting ZEB1

    doi: 10.3892/ol.2019.10697

    Figure Lengend Snippet: Sequences of primers for reverse transcription-quantitative polymerase chain reaction.

    Article Snippet: Western blot analysis Antibodies for E-cadherin (catalog no. 14472; 1:2,000), AXL receptor tyrosine kinase (AXL; catalog no. 8661; 1:2,000), connective tissue growth factor (CTGF; catalog no. 86641; 1:2,000), cysteine rich angiogenic inducer 61 (CYR61; catalog no. 14479; 1:2,000) and ZEB1 (catalog no. 3396; 1:2,000) were purchased from Cell Signaling Technology (Cell Signaling Technology, Inc., Danvers, MA, USA).

    Techniques: Sequencing

    miR-873 represses ZEB1 expression in triple-negative breast cancer cells. (A) Overexpression of miR-873 decreased ZEB1 mRNA level in MDA-MB-231 and BT549 cells. Overexpression of miR-873 decreased ZEB1 protein level and elevated E-cadherin protein level in (B) MDA-MB-231 and (C) BT549 cells. Overexpression of miR-873 decreased AXL, CTGF and CYR61 mRNA levels in (D) MDA-MB-231 and (E) BT549 cells. Overexpression of miR-873 decreased AXL, CTGF and CYR61 protein levels in (F) MDA-MB-231 and (G) BT549 cells. *P<0.05, **P<0.01 and ***P<0.0001 vs. miR-NC mimics. ZEB1, zinc finger E-box binding homeobox 1; NC, negative control; AXL, AXL receptor tyrosine kinase; CTGF, connective tissue growth factor; CYR61, cysteine rich angiogenic inducer 61; miR, microRNA.

    Journal: Oncology Letters

    Article Title: Loss of miR-873 contributes to gemcitabine resistance in triple-negative breast cancer via targeting ZEB1

    doi: 10.3892/ol.2019.10697

    Figure Lengend Snippet: miR-873 represses ZEB1 expression in triple-negative breast cancer cells. (A) Overexpression of miR-873 decreased ZEB1 mRNA level in MDA-MB-231 and BT549 cells. Overexpression of miR-873 decreased ZEB1 protein level and elevated E-cadherin protein level in (B) MDA-MB-231 and (C) BT549 cells. Overexpression of miR-873 decreased AXL, CTGF and CYR61 mRNA levels in (D) MDA-MB-231 and (E) BT549 cells. Overexpression of miR-873 decreased AXL, CTGF and CYR61 protein levels in (F) MDA-MB-231 and (G) BT549 cells. *P<0.05, **P<0.01 and ***P<0.0001 vs. miR-NC mimics. ZEB1, zinc finger E-box binding homeobox 1; NC, negative control; AXL, AXL receptor tyrosine kinase; CTGF, connective tissue growth factor; CYR61, cysteine rich angiogenic inducer 61; miR, microRNA.

    Article Snippet: Western blot analysis Antibodies for E-cadherin (catalog no. 14472; 1:2,000), AXL receptor tyrosine kinase (AXL; catalog no. 8661; 1:2,000), connective tissue growth factor (CTGF; catalog no. 86641; 1:2,000), cysteine rich angiogenic inducer 61 (CYR61; catalog no. 14479; 1:2,000) and ZEB1 (catalog no. 3396; 1:2,000) were purchased from Cell Signaling Technology (Cell Signaling Technology, Inc., Danvers, MA, USA).

    Techniques: Expressing, Over Expression, Binding Assay, Negative Control