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Sensitized tissue content levels of dopamine and its metabolites measured in the (a) anterior dorsal striatum and (b) anterior ventral striatum ( NA c) of wild‐type mice ( WT ) and DKO mice ( n = 4–8/group). Tissues were collected 24 h following a challenge injection of saline or methamphetamine ( METH , 5 mg/kg) on day 13 of the locomotor sensitization procedure. Data reported as average percentage change over WT saline‐treated controls and independent two‐way anova analysis was conducted for each <t>analyte.</t> $ p > 0.05, main effect of METH treatment; * p
Analyte, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "Deficits in behavioral sensitization and dopaminergic responses to methamphetamine in adenylyl cyclase 1/8‐deficient mice"

Article Title: Deficits in behavioral sensitization and dopaminergic responses to methamphetamine in adenylyl cyclase 1/8‐deficient mice

Journal: Journal of Neurochemistry

doi: 10.1111/jnc.13235

Sensitized tissue content levels of dopamine and its metabolites measured in the (a) anterior dorsal striatum and (b) anterior ventral striatum ( NA c) of wild‐type mice ( WT ) and DKO mice ( n = 4–8/group). Tissues were collected 24 h following a challenge injection of saline or methamphetamine ( METH , 5 mg/kg) on day 13 of the locomotor sensitization procedure. Data reported as average percentage change over WT saline‐treated controls and independent two‐way anova analysis was conducted for each analyte. $ p > 0.05, main effect of METH treatment; * p
Figure Legend Snippet: Sensitized tissue content levels of dopamine and its metabolites measured in the (a) anterior dorsal striatum and (b) anterior ventral striatum ( NA c) of wild‐type mice ( WT ) and DKO mice ( n = 4–8/group). Tissues were collected 24 h following a challenge injection of saline or methamphetamine ( METH , 5 mg/kg) on day 13 of the locomotor sensitization procedure. Data reported as average percentage change over WT saline‐treated controls and independent two‐way anova analysis was conducted for each analyte. $ p > 0.05, main effect of METH treatment; * p

Techniques Used: Mouse Assay, Injection

Acute tissue content levels of dopamine and its metabolites measured in the (a) anterior dorsal striatum and (b) anterior ventral striatum ( NA c) of wild‐type mice ( WT ) and DKO mice ( n = 6–9/group). Tissues were collected 30 min following an acute saline or methamphetamine ( METH , 5 mg/kg) injection. Data reported as average percentage change over WT saline‐treated controls and independent two‐way anova analysis was conducted for each analyte. $ p > 0.05, $$ p
Figure Legend Snippet: Acute tissue content levels of dopamine and its metabolites measured in the (a) anterior dorsal striatum and (b) anterior ventral striatum ( NA c) of wild‐type mice ( WT ) and DKO mice ( n = 6–9/group). Tissues were collected 30 min following an acute saline or methamphetamine ( METH , 5 mg/kg) injection. Data reported as average percentage change over WT saline‐treated controls and independent two‐way anova analysis was conducted for each analyte. $ p > 0.05, $$ p

Techniques Used: Mouse Assay, Injection

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Centrifugation:

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Mass Spectrometry:

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Incubation:

Article Title: Processing of Neutrophil α-Defensins Does Not Rely on Serine Proteases In Vivo
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Cell Culture:

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Expressing:

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BIA-KA:

Article Title: Exosome purification based on PEG-coated Fe3O4 nanoparticles
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High Performance Liquid Chromatography:

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Flow Cytometry:

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Article Title: Deficits in behavioral sensitization and dopaminergic responses to methamphetamine in adenylyl cyclase 1/8‐deficient mice
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Chromatography:

Article Title: Psidium guajava Linn. leaf extract affects hepatic glucose transporter-2 to attenuate early onset of insulin resistance consequent to high fructose intake: An experimental study
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Gas Chromatography-Mass Spectrometry:

Article Title: Altered gut metabolome contributes to depression-like behaviors in rats exposed to chronic unpredictable mild stress
Article Snippet: Paragraph title: GC–MS ... The solution was subsequently incubated at 70 °C for 1 h, and 50 μl of N,O-bis (trimethylsilyl) tri-fluoroacetamide (including 1% trimethylchlorosilane) was added before another incubation at 40 °C for 1.5 h. One microliter of each analyte was injected in a (10:1) split mode into a trace gas chromatograph coupled with a Polyris Q Ion Trap mass spectrometer (Thermo Fisher Scientific, MA, USA).

Infection:

Article Title: HIV infection-induced transcriptional program in renal tubular epithelial cells activates a CXCR2 driven CD4+ T cell chemotactic response
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Generated:

Article Title: Performance of Multiplex Commercial Kits to Quantify Cytokine and Chemokine Responses in Culture Supernatants from Plasmodium falciparum Stimulations
Article Snippet: As an example of the Bland-Altman plots generated for each cytokine in each tested kit, the graphs for IL-10 and IL-2 are represented in , whereas the rest are shown in Supporting Information ( , FS2, FS3, FS4, FS5, FS6, FS7, FS8, FS9, FS10, FS11, FS12, FS13, FS14, FS15, FS16, FS17, FS18, FS19, FS20, FS21, FS22, FS23, FS24, FS25, FS26, FS27, FS28, FS29, S30). .. This was mainly due to low cytokine and chemokine concentrations in supernatant samples that were below the limits of detection (e.g. IL-2), although in some cases the analyte concentration was over the accurate range (e.g. IL-8 and IL-6 for Invitrogen™).

Tandem Mass Spectroscopy:

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Article Snippet: .. The specific MS/MS transitions for each analyte were optimized using the Analyst software (Applied Biosystems, Foster City, CA) using direct infusion with and without the addition of the LC solvents. .. Initially, MS/MS transitions were identified for each analyte using the automatic optimization features of the Analyst software.

Article Title: Old age-associated phenotypic screening for Alzheimer's disease drug candidates identifies sterubin as a potent neuroprotective compound from Yerba santa
Article Snippet: The analyte was directly infused into a ThermoFisher Q Exactive mass spectrometer (Waltham, MA, USA) via a micro-flow source. .. MS/MS spectra were recorded and averaged for 2 min. After the analysis of the natural compound, spectra were also recorded for the synthetic compounds (homoeriodictyol, hesperitin, and sterubin), all of which possess a predicted precursor mass of MH+ = 303.09.

Injection:

Article Title: Application of a UPLC–MS/MS method to the protein binding study of TM-2 in rat, human and beagle dog plasma
Article Snippet: The analyte was separated on Thermo Syncronis UPLC® C18 (2.1 mm×50 mm, 1.7 µm) with a thermostated column oven setting at 35 ° C. The mobile phase was composed of solvent A (acetonitrile) and solvent B (2 mM ammonium acetate) at the flow rate of 0.2 mL/min according to the following linear gradient: 0–1.5 min, 60%–90% A; 1.5–2.7 min, 90% A; 2.7–2.8 min, 90%–60% A; 2.8–3.5 min, 60% A. .. The autosampler was conditioned at 8 °C, and the injection volume was 5 μL.

Article Title: Altered gut metabolome contributes to depression-like behaviors in rats exposed to chronic unpredictable mild stress
Article Snippet: .. The solution was subsequently incubated at 70 °C for 1 h, and 50 μl of N,O-bis (trimethylsilyl) tri-fluoroacetamide (including 1% trimethylchlorosilane) was added before another incubation at 40 °C for 1.5 h. One microliter of each analyte was injected in a (10:1) split mode into a trace gas chromatograph coupled with a Polyris Q Ion Trap mass spectrometer (Thermo Fisher Scientific, MA, USA). .. Ethyl Chloroformate (ECF) derivatives were separated with a DB-5MS capillary column (30 m × 250 μm i.d., 0.25 μm film thickness; Agilent J & W Scientific, CA, USA).

Article Title: Deficits in behavioral sensitization and dopaminergic responses to methamphetamine in adenylyl cyclase 1/8‐deficient mice
Article Snippet: A guard cell (ESA 5020) was used in‐line before the injection loop and was set at a potential of +350 mV. .. Absolute concentrations for each analyte were corrected for total soluable protein (BCA™ protein kit, Thermo Scientific) and normalized to percent change from the WT saline‐treated control.

Multiplexing:

Article Title: HIV infection-induced transcriptional program in renal tubular epithelial cells activates a CXCR2 driven CD4+ T cell chemotactic response
Article Snippet: .. The protein level of each analyte was determined with multiplexing analysis software provided by eBioscience. .. CD4+ T cell chemotaxis was performed in transwell membrane inserts with 5um pore size (Corning Inc.).

Article Title: HIV infection-induced transcriptional program in renal tubular epithelial cells activates a CXCR2 driven CD4+ T cell chemotactic response
Article Snippet: 25ul of culture supernatant were incubated with a mixture of coated beads for each analyte to be measured according to manufacturer’s instructions (eBioscience, Cat#BMS8420FF). .. The protein level of each analyte was determined with multiplexing analysis software provided by eBioscience.

Radioactivity:

Article Title: Processing of Neutrophil α-Defensins Does Not Rely on Serine Proteases In Vivo
Article Snippet: Small aliquots of eluate were tested for radioactivity on a Fuji BAS2500 PhosphorImager (Fuji Film). .. Processing assay was performed by incubating 35 S-proHNP with analyte in Dulbecco's Phosphate-Buffered Saline (DPBS; Life Technologies) at 37°C.

Isolation:

Article Title: Processing of Neutrophil α-Defensins Does Not Rely on Serine Proteases In Vivo
Article Snippet: Cells were pelleted and the supernatant used for isolation of 35 S-proHNP by affinity chromatography as previously described[ ]. .. Processing assay was performed by incubating 35 S-proHNP with analyte in Dulbecco's Phosphate-Buffered Saline (DPBS; Life Technologies) at 37°C.

Article Title: Old age-associated phenotypic screening for Alzheimer's disease drug candidates identifies sterubin as a potent neuroprotective compound from Yerba santa
Article Snippet: The analyte was directly infused into a ThermoFisher Q Exactive mass spectrometer (Waltham, MA, USA) via a micro-flow source. .. The signal was isolated and subjected to HCD fragmentation at a normalized collision energy setting of 45.

Purification:

Article Title: Exosome purification based on PEG-coated Fe3O4 nanoparticles
Article Snippet: .. Fetal bovine serum (FBS), which was adopted as the analyte for bioassays of the exosome purification, was purchased from Thermo Fisher Scientific (USA). .. Bicinchoninic acid (BCA) was also purchased from Thermo Fisher Scientific.

Article Title: Roles of oxides of nitrogen on quality enhancement of soybean sprout during hydroponic production using plasma discharged water recycling technology
Article Snippet: An aliquot (20 µl) of the analyte passed through HPLC (Thermo Dionex ultimate 3000) fortified with a UV-Vis sensor (264 nm) and a column (C-18 column, inno C18 column 4.6 × 250, 5 µm, Innopia, Korea). .. The composition of mobile phase was purified water with 0.05 M (NH4 )H2 PO4 (A) and methanol (B).

Chloramphenicol Acetyltransferase Assay:

Article Title: HIV infection-induced transcriptional program in renal tubular epithelial cells activates a CXCR2 driven CD4+ T cell chemotactic response
Article Snippet: 25ul of culture supernatant were incubated with a mixture of coated beads for each analyte to be measured according to manufacturer’s instructions (eBioscience, CatBMS8420FF). .. The protein level of each analyte was determined with multiplexing analysis software provided by eBioscience.

Article Title: HIV infection-induced transcriptional program in renal tubular epithelial cells activates a CXCR2 driven CD4+ T cell chemotactic response
Article Snippet: .. 25ul of culture supernatant were incubated with a mixture of coated beads for each analyte to be measured according to manufacturer’s instructions (eBioscience, CatBMS8420FF). ..

Software:

Article Title: Application of a UPLC–MS/MS method to the protein binding study of TM-2 in rat, human and beagle dog plasma
Article Snippet: All data were acquired in centroid mode by the Masslynx™ NT4.1 software and analyzed by QuanLynx™ program (Waters Corp., Milford, MA, USA). .. The analyte was separated on Thermo Syncronis UPLC® C18 (2.1 mm×50 mm, 1.7 µm) with a thermostated column oven setting at 35 ° C. The mobile phase was composed of solvent A (acetonitrile) and solvent B (2 mM ammonium acetate) at the flow rate of 0.2 mL/min according to the following linear gradient: 0–1.5 min, 60%–90% A; 1.5–2.7 min, 90% A; 2.7–2.8 min, 90%–60% A; 2.8–3.5 min, 60% A.

Article Title: A sensitive and specific method for measurement of multiple retinoids in human serum with UHPLC-MS/MS
Article Snippet: .. The specific MS/MS transitions for each analyte were optimized using the Analyst software (Applied Biosystems, Foster City, CA) using direct infusion with and without the addition of the LC solvents. .. Initially, MS/MS transitions were identified for each analyte using the automatic optimization features of the Analyst software.

Article Title: Psidium guajava Linn. leaf extract affects hepatic glucose transporter-2 to attenuate early onset of insulin resistance consequent to high fructose intake: An experimental study
Article Snippet: Chromquest software version 4.1 (Thermo Electron Corp. Waltham, MA, USA) was used to control all parameters of HPLC. .. Tandem mass spectrometric detection of analyte and internal standard (IS) was carried out using Applied Bio Systems 400 Q TRAP triple quadrupole instrument equipped with a TurbolonSpray (ESI) source (ABS Biosystems, Foster City, CA, USA) that operated in the positive ion mode.

Article Title: HIV infection-induced transcriptional program in renal tubular epithelial cells activates a CXCR2 driven CD4+ T cell chemotactic response
Article Snippet: .. The protein level of each analyte was determined with multiplexing analysis software provided by eBioscience. .. CD4+ T cell chemotaxis was performed in transwell membrane inserts with 5um pore size (Corning Inc.).

Article Title: Deficits in behavioral sensitization and dopaminergic responses to methamphetamine in adenylyl cyclase 1/8‐deficient mice
Article Snippet: Peak area for each analyte was integrated and quantified against known standards using Shimadzu LC solutions software, Nakagyo‐ku, Kyoto, Japan; (dialysis and sensitized samples) or Dionex Chromeleon 7 software (acute samples). .. Absolute concentrations for each analyte were corrected for total soluable protein (BCA™ protein kit, Thermo Scientific) and normalized to percent change from the WT saline‐treated control.

Article Title: Protective Vaccine Efficacy of the Complete Form of PPE39 Protein from Mycobacterium tuberculosis Beijing/K Strain in Mice
Article Snippet: .. Standard curves for each analyte were obtained by the best fit of the data points using FlowCytomix Pro software (eBioscience), and values outside the standard curve were adjusted by setting the minimum and maximum values. .. A total of 21 peptides which overlap by six amino acids were synthesized for determination of potential epitope sites (GenScript, Piscataway, NJ, USA) (Fig. S3B).

Article Title: HIV infection-induced transcriptional program in renal tubular epithelial cells activates a CXCR2 driven CD4+ T cell chemotactic response
Article Snippet: 25ul of culture supernatant were incubated with a mixture of coated beads for each analyte to be measured according to manufacturer’s instructions (eBioscience, Cat#BMS8420FF). .. The protein level of each analyte was determined with multiplexing analysis software provided by eBioscience.

Multiplex Assay:

Article Title: Protective Vaccine Efficacy of the Complete Form of PPE39 Protein from Mycobacterium tuberculosis Beijing/K Strain in Mice
Article Snippet: Paragraph title: Multiplex bead array. ... Standard curves for each analyte were obtained by the best fit of the data points using FlowCytomix Pro software (eBioscience), and values outside the standard curve were adjusted by setting the minimum and maximum values.

Affinity Chromatography:

Article Title: Processing of Neutrophil α-Defensins Does Not Rely on Serine Proteases In Vivo
Article Snippet: Cells were pelleted and the supernatant used for isolation of 35 S-proHNP by affinity chromatography as previously described[ ]. .. Processing assay was performed by incubating 35 S-proHNP with analyte in Dulbecco's Phosphate-Buffered Saline (DPBS; Life Technologies) at 37°C.

Concentration Assay:

Article Title: Processing of Neutrophil α-Defensins Does Not Rely on Serine Proteases In Vivo
Article Snippet: The cells were pelleted by centrifugation, resuspended at 5x106 cells/mL in DMEM as above to which 35 S-methionine/cysteine (NEG772002MC; Perkin Elmer) had been added to a final concentration of 100 μCi/mL, and pulsed for overnight in a humidified incubator with 5% CO2 at 37°C. .. Processing assay was performed by incubating 35 S-proHNP with analyte in Dulbecco's Phosphate-Buffered Saline (DPBS; Life Technologies) at 37°C.

Article Title: Performance of Multiplex Commercial Kits to Quantify Cytokine and Chemokine Responses in Culture Supernatants from Plasmodium falciparum Stimulations
Article Snippet: .. This was mainly due to low cytokine and chemokine concentrations in supernatant samples that were below the limits of detection (e.g. IL-2), although in some cases the analyte concentration was over the accurate range (e.g. IL-8 and IL-6 for Invitrogen™). ..

Staining:

Article Title: Processing of Neutrophil α-Defensins Does Not Rely on Serine Proteases In Vivo
Article Snippet: Processing assay was performed by incubating 35 S-proHNP with analyte in Dulbecco's Phosphate-Buffered Saline (DPBS; Life Technologies) at 37°C. .. This was subjected 16% SDS-Tricine-PAGE, stained with Coomassie, soaked in Amplify (NAMP100V; GE Healthcare), placed in a Fuji BAS cassette (Fuji Film), and developed by a Fuji BAS2500 PhosphorImager.

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    https://www.bioz.com/result/sorvall discovery m150 se analytical ultracentrifuge/product/Thermo Fisher
    Average 90 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    sorvall discovery m150 se analytical ultracentrifuge - by Bioz Stars, 2020-01
    90/100 stars
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    The Thermo Scientific Dionex IonPac Anion Analyte Trap Column will remove all the analyte anions from a sample in RFIC systems with Eluent Regeneration
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