Structured Review

Thermo Fisher analyte
Recovery of fatty acyl-CoAs at each step of the extraction of MCF7 cells (A) and comparison of peak areas for analytes in solvent versus cell extracts (B). A: Approximately 10 6 MCF7 cells were spiked with odd-chain-length fatty acyl-CoAs and extracted as described in Experimental Procedures, with the exception that each of the reextractions was collected and individually analyzed by LC-ESI MS/MS as described (white, dark gray, and light gray bars). The lower layer of the extraction was reextracted a fourth time (black bars). After integrating peak areas for each <t>analyte,</t> the areas in all three extractions were summed, and the percentage of the total recovered in each extraction is shown. Results are means ± SD of analyses of three replicate Petri dishes. B: Two internal standards (C15:0- and C25:0-CoAs, in the amounts shown) were analyzed alone versus as spiked into a RAW264.7 extract then analyzed by LC-ESI MS/MS. The circles are the C15:0-CoA and the squares are the C25:0-CoA; closed symbols are the analytes in solvent alone, the open symbols are the analytes spiked into the cell extract.
Analyte, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 38 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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analyte - by Bioz Stars, 2020-07
92/100 stars

Images

1) Product Images from "Quantitation of fatty acyl-coenzyme As in mammalian cells by liquid chromatography-electrospray ionization tandem mass spectrometry *"

Article Title: Quantitation of fatty acyl-coenzyme As in mammalian cells by liquid chromatography-electrospray ionization tandem mass spectrometry *

Journal: Journal of Lipid Research

doi: 10.1194/jlr.D800001-JLR200

Recovery of fatty acyl-CoAs at each step of the extraction of MCF7 cells (A) and comparison of peak areas for analytes in solvent versus cell extracts (B). A: Approximately 10 6 MCF7 cells were spiked with odd-chain-length fatty acyl-CoAs and extracted as described in Experimental Procedures, with the exception that each of the reextractions was collected and individually analyzed by LC-ESI MS/MS as described (white, dark gray, and light gray bars). The lower layer of the extraction was reextracted a fourth time (black bars). After integrating peak areas for each analyte, the areas in all three extractions were summed, and the percentage of the total recovered in each extraction is shown. Results are means ± SD of analyses of three replicate Petri dishes. B: Two internal standards (C15:0- and C25:0-CoAs, in the amounts shown) were analyzed alone versus as spiked into a RAW264.7 extract then analyzed by LC-ESI MS/MS. The circles are the C15:0-CoA and the squares are the C25:0-CoA; closed symbols are the analytes in solvent alone, the open symbols are the analytes spiked into the cell extract.
Figure Legend Snippet: Recovery of fatty acyl-CoAs at each step of the extraction of MCF7 cells (A) and comparison of peak areas for analytes in solvent versus cell extracts (B). A: Approximately 10 6 MCF7 cells were spiked with odd-chain-length fatty acyl-CoAs and extracted as described in Experimental Procedures, with the exception that each of the reextractions was collected and individually analyzed by LC-ESI MS/MS as described (white, dark gray, and light gray bars). The lower layer of the extraction was reextracted a fourth time (black bars). After integrating peak areas for each analyte, the areas in all three extractions were summed, and the percentage of the total recovered in each extraction is shown. Results are means ± SD of analyses of three replicate Petri dishes. B: Two internal standards (C15:0- and C25:0-CoAs, in the amounts shown) were analyzed alone versus as spiked into a RAW264.7 extract then analyzed by LC-ESI MS/MS. The circles are the C15:0-CoA and the squares are the C25:0-CoA; closed symbols are the analytes in solvent alone, the open symbols are the analytes spiked into the cell extract.

Techniques Used: Mass Spectrometry

LC-ESI MS/MS with neutral loss scanning (507.0 Da) of MCF7 cell extract. An extract of 2 × 10 7 MCF7 cells was analyzed by LC-ESI MS/MS as described in Experimental Procedures. A shows the total ion chromatogram for a neutral loss scan (507.0 Da); the chromatographic time is divided into three periods with distinct m/z scan, DP, CE, and CXP ranges. Dashed boxes on the total ion chromatogram labeled B–D indicate the time window from which the neutral loss scans in panels B–D are derived. The abcissae of B–D indicate the m/z range of the cognate neutral loss scan, and settings for DP, CE, and CXP ranged from those appropriate for the analyte of the lowest m/z value to those appropriate for the analyte of the highest m/z value within that scan range. The ordinates of B–D are normalized to their respective base peaks, not to the total ion chromatogram intensity of A.
Figure Legend Snippet: LC-ESI MS/MS with neutral loss scanning (507.0 Da) of MCF7 cell extract. An extract of 2 × 10 7 MCF7 cells was analyzed by LC-ESI MS/MS as described in Experimental Procedures. A shows the total ion chromatogram for a neutral loss scan (507.0 Da); the chromatographic time is divided into three periods with distinct m/z scan, DP, CE, and CXP ranges. Dashed boxes on the total ion chromatogram labeled B–D indicate the time window from which the neutral loss scans in panels B–D are derived. The abcissae of B–D indicate the m/z range of the cognate neutral loss scan, and settings for DP, CE, and CXP ranged from those appropriate for the analyte of the lowest m/z value to those appropriate for the analyte of the highest m/z value within that scan range. The ordinates of B–D are normalized to their respective base peaks, not to the total ion chromatogram intensity of A.

Techniques Used: Mass Spectrometry, Labeling, Derivative Assay

2) Product Images from "Automated Detection of Inaccurate and Imprecise Transitions in Peptide Quantification by Multiple Reaction Monitoring Mass Spectrometry"

Article Title: Automated Detection of Inaccurate and Imprecise Transitions in Peptide Quantification by Multiple Reaction Monitoring Mass Spectrometry

Journal: Clinical chemistry

doi: 10.1373/clinchem.2009.138420

Examples of MRM-MS spectra of analyte and SIS peptides in the absence (A) and presence (B) of a coeluting interference (A), MRM mass spectra of analyte peptide (LFTGHPETLEK, blue spectra) and the corresponding heavy SIS peptide (red spectra) in digested plasma matrix. Three separate LC-MRM-MS runs are shown with a fixed amount of SIS peptide (50 fmol) and varying analyte peptide (1, 46, and 500 fmol) spiked into 1 μ g digested plasma. Although the absolute intensities of the fragments ( y axes) vary with concentration and potentially as a function of sample introduction into the mass spectrometer, the relative intensities of the product ions at m / z 506.3, 579.8, and 716.4 maintain a constant relationship with one another. Furthermore, the relative intensities of the analyte fragment ions agree precisely with the intensities of the fragment ions from the heavy peptide. (B), Example of an interference in peptide ESDTSYVSLK (transition y 5 , m / z 564.8/609.4) in digested plasma, which gives rise to an altered MRM-MS profile. The XICs are shown for the 3 transitions monitored for analyte peptide (top) and SIS peptide (bottom).
Figure Legend Snippet: Examples of MRM-MS spectra of analyte and SIS peptides in the absence (A) and presence (B) of a coeluting interference (A), MRM mass spectra of analyte peptide (LFTGHPETLEK, blue spectra) and the corresponding heavy SIS peptide (red spectra) in digested plasma matrix. Three separate LC-MRM-MS runs are shown with a fixed amount of SIS peptide (50 fmol) and varying analyte peptide (1, 46, and 500 fmol) spiked into 1 μ g digested plasma. Although the absolute intensities of the fragments ( y axes) vary with concentration and potentially as a function of sample introduction into the mass spectrometer, the relative intensities of the product ions at m / z 506.3, 579.8, and 716.4 maintain a constant relationship with one another. Furthermore, the relative intensities of the analyte fragment ions agree precisely with the intensities of the fragment ions from the heavy peptide. (B), Example of an interference in peptide ESDTSYVSLK (transition y 5 , m / z 564.8/609.4) in digested plasma, which gives rise to an altered MRM-MS profile. The XICs are shown for the 3 transitions monitored for analyte peptide (top) and SIS peptide (bottom).

Techniques Used: Mass Spectrometry, Concentration Assay

Examples of sources of error in quantitative measurements by MRM-MS (A–E), XICs demonstrating common causes for incorrect quantification in SID-MRM-MS. (A), Example of poor chromatographic peak shape causing inconsistent automatic peak integration. (B), Presence of a peak with a closely eluting interference causing inconsistent integration. (C), Detector saturation at high analyte concentration. (D), Peak with
Figure Legend Snippet: Examples of sources of error in quantitative measurements by MRM-MS (A–E), XICs demonstrating common causes for incorrect quantification in SID-MRM-MS. (A), Example of poor chromatographic peak shape causing inconsistent automatic peak integration. (B), Presence of a peak with a closely eluting interference causing inconsistent integration. (C), Detector saturation at high analyte concentration. (D), Peak with

Techniques Used: Mass Spectrometry, Concentration Assay

Analysis work flow for isotope dilution MRM-MS data with and without the use of AuDIT After LC-MRM-MS analysis of samples, transition peaks are identified and integrated with software from either the mass spectrometer vendor or another supplier. (A), Flow of data with use of the automated algorithm. The statistical test identifies problem transitions from the variation in the relative ratios for the analyte and the SIS. The CV of the PARs is used as a filter to flag transitions with unacceptably large variation. (B), The current standard practice of careful manual inspection of all transitions by an expert.
Figure Legend Snippet: Analysis work flow for isotope dilution MRM-MS data with and without the use of AuDIT After LC-MRM-MS analysis of samples, transition peaks are identified and integrated with software from either the mass spectrometer vendor or another supplier. (A), Flow of data with use of the automated algorithm. The statistical test identifies problem transitions from the variation in the relative ratios for the analyte and the SIS. The CV of the PARs is used as a filter to flag transitions with unacceptably large variation. (B), The current standard practice of careful manual inspection of all transitions by an expert.

Techniques Used: Flow Cytometry, Isotope Dilution, Mass Spectrometry, Software

ROC curve and sensitivity–specificity plots summarizing performance of AuDIT in identifying inaccurate and imprecise transitions, as evaluated by an expert AuDIT uses the t -test P value and the CV of the PAR (ratio of analyte peak area to SIS peak area) to detect problem transitions. (A), Both the P value and the CV are required to achieve acceptable performance (i.e., as indicated by AUC values in parentheses). (B), Specificity and sensitivity values achieved as the P value threshold is varied from 0 to 1 (with a fixed CV threshold of 20%). The chosen P value threshold of 10 −5 used for all of the analyzed data is indicated by the red circle (sensitivity, 98%; specificity, 97%). The rainbow color bar (right y axis) keys the location of the P value threshold on the sensitivity–specificity curve.
Figure Legend Snippet: ROC curve and sensitivity–specificity plots summarizing performance of AuDIT in identifying inaccurate and imprecise transitions, as evaluated by an expert AuDIT uses the t -test P value and the CV of the PAR (ratio of analyte peak area to SIS peak area) to detect problem transitions. (A), Both the P value and the CV are required to achieve acceptable performance (i.e., as indicated by AUC values in parentheses). (B), Specificity and sensitivity values achieved as the P value threshold is varied from 0 to 1 (with a fixed CV threshold of 20%). The chosen P value threshold of 10 −5 used for all of the analyzed data is indicated by the red circle (sensitivity, 98%; specificity, 97%). The rainbow color bar (right y axis) keys the location of the P value threshold on the sensitivity–specificity curve.

Techniques Used:

3) Product Images from "Deficits in behavioral sensitization and dopaminergic responses to methamphetamine in adenylyl cyclase 1/8‐deficient mice"

Article Title: Deficits in behavioral sensitization and dopaminergic responses to methamphetamine in adenylyl cyclase 1/8‐deficient mice

Journal: Journal of Neurochemistry

doi: 10.1111/jnc.13235

Sensitized tissue content levels of dopamine and its metabolites measured in the (a) anterior dorsal striatum and (b) anterior ventral striatum ( NA c) of wild‐type mice ( WT ) and DKO mice ( n = 4–8/group). Tissues were collected 24 h following a challenge injection of saline or methamphetamine ( METH , 5 mg/kg) on day 13 of the locomotor sensitization procedure. Data reported as average percentage change over WT saline‐treated controls and independent two‐way anova analysis was conducted for each analyte. $ p > 0.05, main effect of METH treatment; * p
Figure Legend Snippet: Sensitized tissue content levels of dopamine and its metabolites measured in the (a) anterior dorsal striatum and (b) anterior ventral striatum ( NA c) of wild‐type mice ( WT ) and DKO mice ( n = 4–8/group). Tissues were collected 24 h following a challenge injection of saline or methamphetamine ( METH , 5 mg/kg) on day 13 of the locomotor sensitization procedure. Data reported as average percentage change over WT saline‐treated controls and independent two‐way anova analysis was conducted for each analyte. $ p > 0.05, main effect of METH treatment; * p

Techniques Used: Mouse Assay, Injection

Acute tissue content levels of dopamine and its metabolites measured in the (a) anterior dorsal striatum and (b) anterior ventral striatum ( NA c) of wild‐type mice ( WT ) and DKO mice ( n = 6–9/group). Tissues were collected 30 min following an acute saline or methamphetamine ( METH , 5 mg/kg) injection. Data reported as average percentage change over WT saline‐treated controls and independent two‐way anova analysis was conducted for each analyte. $ p > 0.05, $$ p
Figure Legend Snippet: Acute tissue content levels of dopamine and its metabolites measured in the (a) anterior dorsal striatum and (b) anterior ventral striatum ( NA c) of wild‐type mice ( WT ) and DKO mice ( n = 6–9/group). Tissues were collected 30 min following an acute saline or methamphetamine ( METH , 5 mg/kg) injection. Data reported as average percentage change over WT saline‐treated controls and independent two‐way anova analysis was conducted for each analyte. $ p > 0.05, $$ p

Techniques Used: Mouse Assay, Injection

4) Product Images from "Quantitation of fatty acyl-coenzyme As in mammalian cells by liquid chromatography-electrospray ionization tandem mass spectrometry *"

Article Title: Quantitation of fatty acyl-coenzyme As in mammalian cells by liquid chromatography-electrospray ionization tandem mass spectrometry *

Journal: Journal of Lipid Research

doi: 10.1194/jlr.D800001-JLR200

Recovery of fatty acyl-CoAs at each step of the extraction of MCF7 cells (A) and comparison of peak areas for analytes in solvent versus cell extracts (B). A: Approximately 10 6 MCF7 cells were spiked with odd-chain-length fatty acyl-CoAs and extracted as described in Experimental Procedures, with the exception that each of the reextractions was collected and individually analyzed by LC-ESI MS/MS as described (white, dark gray, and light gray bars). The lower layer of the extraction was reextracted a fourth time (black bars). After integrating peak areas for each analyte, the areas in all three extractions were summed, and the percentage of the total recovered in each extraction is shown. Results are means ± SD of analyses of three replicate Petri dishes. B: Two internal standards (C15:0- and C25:0-CoAs, in the amounts shown) were analyzed alone versus as spiked into a RAW264.7 extract then analyzed by LC-ESI MS/MS. The circles are the C15:0-CoA and the squares are the C25:0-CoA; closed symbols are the analytes in solvent alone, the open symbols are the analytes spiked into the cell extract.
Figure Legend Snippet: Recovery of fatty acyl-CoAs at each step of the extraction of MCF7 cells (A) and comparison of peak areas for analytes in solvent versus cell extracts (B). A: Approximately 10 6 MCF7 cells were spiked with odd-chain-length fatty acyl-CoAs and extracted as described in Experimental Procedures, with the exception that each of the reextractions was collected and individually analyzed by LC-ESI MS/MS as described (white, dark gray, and light gray bars). The lower layer of the extraction was reextracted a fourth time (black bars). After integrating peak areas for each analyte, the areas in all three extractions were summed, and the percentage of the total recovered in each extraction is shown. Results are means ± SD of analyses of three replicate Petri dishes. B: Two internal standards (C15:0- and C25:0-CoAs, in the amounts shown) were analyzed alone versus as spiked into a RAW264.7 extract then analyzed by LC-ESI MS/MS. The circles are the C15:0-CoA and the squares are the C25:0-CoA; closed symbols are the analytes in solvent alone, the open symbols are the analytes spiked into the cell extract.

Techniques Used: Mass Spectrometry

LC-ESI MS/MS with neutral loss scanning (507.0 Da) of MCF7 cell extract. An extract of 2 × 10 7 MCF7 cells was analyzed by LC-ESI MS/MS as described in Experimental Procedures. A shows the total ion chromatogram for a neutral loss scan (507.0 Da); the chromatographic time is divided into three periods with distinct m/z scan, DP, CE, and CXP ranges. Dashed boxes on the total ion chromatogram labeled B–D indicate the time window from which the neutral loss scans in panels B–D are derived. The abcissae of B–D indicate the m/z range of the cognate neutral loss scan, and settings for DP, CE, and CXP ranged from those appropriate for the analyte of the lowest m/z value to those appropriate for the analyte of the highest m/z value within that scan range. The ordinates of B–D are normalized to their respective base peaks, not to the total ion chromatogram intensity of A.
Figure Legend Snippet: LC-ESI MS/MS with neutral loss scanning (507.0 Da) of MCF7 cell extract. An extract of 2 × 10 7 MCF7 cells was analyzed by LC-ESI MS/MS as described in Experimental Procedures. A shows the total ion chromatogram for a neutral loss scan (507.0 Da); the chromatographic time is divided into three periods with distinct m/z scan, DP, CE, and CXP ranges. Dashed boxes on the total ion chromatogram labeled B–D indicate the time window from which the neutral loss scans in panels B–D are derived. The abcissae of B–D indicate the m/z range of the cognate neutral loss scan, and settings for DP, CE, and CXP ranged from those appropriate for the analyte of the lowest m/z value to those appropriate for the analyte of the highest m/z value within that scan range. The ordinates of B–D are normalized to their respective base peaks, not to the total ion chromatogram intensity of A.

Techniques Used: Mass Spectrometry, Labeling, Derivative Assay

5) Product Images from "Cationic Xylene Tag for Increasing Sensitivity in Mass Spectrometry"

Article Title: Cationic Xylene Tag for Increasing Sensitivity in Mass Spectrometry

Journal: Journal of the American Society for Mass Spectrometry

doi: 10.1007/s13361-015-1200-4

Reaction scheme for tagging an analyte having an active hydrogen with CAX-B.
Figure Legend Snippet: Reaction scheme for tagging an analyte having an active hydrogen with CAX-B.

Techniques Used:

Related Articles

Software:

Article Title: Quantitation of fatty acyl-coenzyme As in mammalian cells by liquid chromatography-electrospray ionization tandem mass spectrometry *
Article Snippet: .. The amount of each analyte of interest was calculated as follows: 1 ) LC chromatogram peaks for internal standards and endogenous fatty acyl-CoAs were integrated using Analyst 1.4.2 software (Applied Biosystems) and peak areas were copied to spreadsheets; 2 ) for each endogenous analyte, a cognate internal standard was selected based upon either similarity of acyl chain length or similarity of LC elution time (whichever is most appropriate); and 3 ) the following formula was used to calculate pmol of analyte (pmola ): where Aa = analyte peak area, Ais = internal standard peak area, pmolis = spiked pmol of internal standard, Mis = slope of the linear regression of the internal standard's calibration curve, and Ma = slope of the linear regression of the analyte's calibration curve. ..

Article Title: Protective Vaccine Efficacy of the Complete Form of PPE39 Protein from Mycobacterium tuberculosis Beijing/K Strain in Mice
Article Snippet: .. Standard curves for each analyte were obtained by the best fit of the data points using FlowCytomix Pro software (eBioscience), and values outside the standard curve were adjusted by setting the minimum and maximum values. .. A total of 21 peptides which overlap by six amino acids were synthesized for determination of potential epitope sites (GenScript, Piscataway, NJ, USA) (Fig. S3B).

other:

Article Title: Cationic Xylene Tag for Increasing Sensitivity in Mass Spectrometry
Article Snippet: Six μL of CAX-B solution (CAX-B at 1 mg/mL, and Et3 N at 10 μL/mL in 50% ACN) was added to a vial containing evaporated analyte, and after 16 h at 38°C CapLC/MALDI-TOF/TOF-MS was done using the following LC conditions: Dionex Ultimate, with 0.3 × 150 mm, 2 μm Acclaim PepMed C18 column, mobile phase: A, H2 O with 0.1% TFA, 2% ACN; B, ACN with 0.1% TFA; gradient up to 12% B over 4 min, then up to 90% B in 40 min with collection of 3 droplets/min onto a MALDI plate, followed by manual addition of CCA Matrix Solution and MALDI-TOF/TOF-MS.

Mass Spectrometry:

Article Title: Burkholderia bacteria use chemotaxis to find social amoeba Dictyostelium discoideum hosts
Article Snippet: .. The analyte was ionised and introduced into the mass spectrometer by the Thermo Nanospray Flex Ion Source (Waltham, MA) in positive mode. ..

Article Title: Automated Detection of Inaccurate and Imprecise Transitions in Peptide Quantification by Multiple Reaction Monitoring Mass Spectrometry
Article Snippet: .. We monitored 5 MRM transitions for each analyte and SIS peptide on a 5500 Q TRAP mass spectrometer (Applied Biosystems) by scheduled MRM with a target cycle time of 0.5 s, a retention-time window of 90 s, and an interscan delay of 3 ms. ..

BIA-KA:

Article Title: Deficits in behavioral sensitization and dopaminergic responses to methamphetamine in adenylyl cyclase 1/8‐deficient mice
Article Snippet: .. Absolute concentrations for each analyte were corrected for total soluable protein (BCA™ protein kit, Thermo Scientific) and normalized to percent change from the WT saline‐treated control. .. Immunoblot analysis Equal amounts of whole cell protein lysates (10–20 μg) were separated using sodium dodecyl sulfate–polyacrylamide gel electrophoresis (4–12% Bis‐Tris NuPAGE gel) and transferred to a nitrocellulose membrane.

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  • 93
    Thermo Fisher c18 analytical column
    HPLC profiles of (A) a standard sample of 14 C-1,25(OH) 2 D 3 -3-BE, and (B) organic extract of a sample of human serum, spiked with 14 C-1,25(OH) 2 D 3 -3-BE. Conditions: <t>C18</t> column, 5% H 2 O-MeOH: mobile phase, on-line radioactivity-detection. The experiment was
    C18 Analytical Column, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/c18 analytical column/product/Thermo Fisher
    Average 93 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    c18 analytical column - by Bioz Stars, 2020-07
    93/100 stars
      Buy from Supplier

    88
    Thermo Fisher 3 hydroxy nonanoic acid analytical standard
    Characterization of <t>3-hydroxy</t> fatty acids in C. neoformans UOFS Y-1378 and C. gattii R265. (A) The EIC (top left graphic) obtained for the analytical standard compound (3-hydroxy nonanoic acid) and its corresponding mass spectrum (top right graphic). (B) The EIC (middle left graphic) obtained for C. neoformans analyte and its corresponding mass spectrum (middle right graphic). C. neoformans had a similar profile (i.r.o. retention time and diagnostic peaks) suggesting this fungus produces 3-hydroxy nonanoic acid. (C) The EIC (bottom left graphic) obtained for C. gattii and its corresponding mass spectrum (bottom right graphic). C. gattii produced an unknown metabolite, which had a similar retention time to the analytical standard compound. However, we could not detect diagnostic peaks ([M-H] - , [M + Na + M-H] - and [M + M-H] - ) that are characteristic of our metabolite of interest (3-hydroxy nonanoic acid).
    3 Hydroxy Nonanoic Acid Analytical Standard, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/3 hydroxy nonanoic acid analytical standard/product/Thermo Fisher
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    3 hydroxy nonanoic acid analytical standard - by Bioz Stars, 2020-07
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    92
    Thermo Fisher igg analytical
    Comparing the quality of a human IgG1 purified by (i) conventional column, (ii) automated tip column and (iii) batch mode. (a) Analytical SEC profiles of purified IgG1. (b) Reduced <t>SDS-PAGE,</t> M: Mark 12 standard. (c) Non-reduced SDS-PAGE. (d) LC-MS intact mass analysis of light chain. (e) LC-MS intact mass analysis of heavy chain. No significant difference in purity or measured masses was observed in purified <t>IgG</t> from automated processes compared to a conventional column process. Glycosylation variants abbreviations: Man5 (Man 5 GlcNAc 2 ), G0F (GlcNAc 2 Man 3 GlcNAc 2 ), G1F (GalGlcNAc 2 Man 3 (Fuc)GlcNAc 2 ), where: Man (mannose), GlcNAc (N-acetylglucosamine), Gal (Galactose), Fuc (Fucose).
    Igg Analytical, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/igg analytical/product/Thermo Fisher
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    igg analytical - by Bioz Stars, 2020-07
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    Image Search Results


    HPLC profiles of (A) a standard sample of 14 C-1,25(OH) 2 D 3 -3-BE, and (B) organic extract of a sample of human serum, spiked with 14 C-1,25(OH) 2 D 3 -3-BE. Conditions: C18 column, 5% H 2 O-MeOH: mobile phase, on-line radioactivity-detection. The experiment was

    Journal: Cancer prevention research (Philadelphia, Pa.)

    Article Title: A VITAMIN D RECEPTOR-ALKYLATING DERIVATIVE OF 1?, 25-DIHYDROXYVITAMIN D3 INHIBITS GROWTH OF HUMAN KIDNEY CANCER CELLS AND SUPPRESSES TUMOR-GROWTH

    doi: 10.1158/1940-6207.CAPR-10-0122

    Figure Lengend Snippet: HPLC profiles of (A) a standard sample of 14 C-1,25(OH) 2 D 3 -3-BE, and (B) organic extract of a sample of human serum, spiked with 14 C-1,25(OH) 2 D 3 -3-BE. Conditions: C18 column, 5% H 2 O-MeOH: mobile phase, on-line radioactivity-detection. The experiment was

    Article Snippet: Combined organic extract was dried under argon and the residue was re-dissolved in a small volume of 5% H2 O-MeOH, and analyzed in an Agilent 1100 Series HPLC system (Thermo-Fisher, Waltham, MA), connected to a Packard Flow Scintillation Analyzer (Model no. 150TR, Meriden, CT), using 5% H2 O-MeOH as mobile phase, flow rate1.5 ml/min, detection 265 nm (for non-radioactive materials), Agilent C18 analytical column (Thermo-Fisher, Waltham, MA).

    Techniques: High Performance Liquid Chromatography, Radioactivity

    Characterization of 3-hydroxy fatty acids in C. neoformans UOFS Y-1378 and C. gattii R265. (A) The EIC (top left graphic) obtained for the analytical standard compound (3-hydroxy nonanoic acid) and its corresponding mass spectrum (top right graphic). (B) The EIC (middle left graphic) obtained for C. neoformans analyte and its corresponding mass spectrum (middle right graphic). C. neoformans had a similar profile (i.r.o. retention time and diagnostic peaks) suggesting this fungus produces 3-hydroxy nonanoic acid. (C) The EIC (bottom left graphic) obtained for C. gattii and its corresponding mass spectrum (bottom right graphic). C. gattii produced an unknown metabolite, which had a similar retention time to the analytical standard compound. However, we could not detect diagnostic peaks ([M-H] - , [M + Na + M-H] - and [M + M-H] - ) that are characteristic of our metabolite of interest (3-hydroxy nonanoic acid).

    Journal: Frontiers in Microbiology

    Article Title: Cryptococcal 3-Hydroxy Fatty Acids Protect Cells Against Amoebal Phagocytosis

    doi: 10.3389/fmicb.2015.01351

    Figure Lengend Snippet: Characterization of 3-hydroxy fatty acids in C. neoformans UOFS Y-1378 and C. gattii R265. (A) The EIC (top left graphic) obtained for the analytical standard compound (3-hydroxy nonanoic acid) and its corresponding mass spectrum (top right graphic). (B) The EIC (middle left graphic) obtained for C. neoformans analyte and its corresponding mass spectrum (middle right graphic). C. neoformans had a similar profile (i.r.o. retention time and diagnostic peaks) suggesting this fungus produces 3-hydroxy nonanoic acid. (C) The EIC (bottom left graphic) obtained for C. gattii and its corresponding mass spectrum (bottom right graphic). C. gattii produced an unknown metabolite, which had a similar retention time to the analytical standard compound. However, we could not detect diagnostic peaks ([M-H] - , [M + Na + M-H] - and [M + M-H] - ) that are characteristic of our metabolite of interest (3-hydroxy nonanoic acid).

    Article Snippet: The relative peak areas of 3-hydroxy nonanoic acid (monoisotopic mass: 174.125595 Da, retention time (RT) 3.7 min) were obtained for each sample and the 3-hydroxy nonanoic acid analytical standard in the Qual Browser function of the Xcalibur software package (Thermo-Fisher Ltd.).

    Techniques: Diagnostic Assay, Produced

    The results of the phagocytosis assay of cryptococcal cells co-cultured with amoeba. Through using the phagocytosis stain, pHrodo TM Green Zymosan A BioParticles, the appetite of amoebae to internalize C. neoformans UOFS Y-1378, C. neoformans LMPE 046 and C. gattii R265, in the presence and absence of 3-hydroxy fatty acids, was measured after 2 h (A) and 6 h (B) of co-culturing. At both time intervals, C. neoformans UOFS Y-1378 cells were more resistant ( p

    Journal: Frontiers in Microbiology

    Article Title: Cryptococcal 3-Hydroxy Fatty Acids Protect Cells Against Amoebal Phagocytosis

    doi: 10.3389/fmicb.2015.01351

    Figure Lengend Snippet: The results of the phagocytosis assay of cryptococcal cells co-cultured with amoeba. Through using the phagocytosis stain, pHrodo TM Green Zymosan A BioParticles, the appetite of amoebae to internalize C. neoformans UOFS Y-1378, C. neoformans LMPE 046 and C. gattii R265, in the presence and absence of 3-hydroxy fatty acids, was measured after 2 h (A) and 6 h (B) of co-culturing. At both time intervals, C. neoformans UOFS Y-1378 cells were more resistant ( p

    Article Snippet: The relative peak areas of 3-hydroxy nonanoic acid (monoisotopic mass: 174.125595 Da, retention time (RT) 3.7 min) were obtained for each sample and the 3-hydroxy nonanoic acid analytical standard in the Qual Browser function of the Xcalibur software package (Thermo-Fisher Ltd.).

    Techniques: Phagocytosis Assay, Cell Culture, Staining

    The results of the survival of aspirin-treated C. neoformans UOFS Y-1378 cells and non-treated C. neoformans UOFS Y-1378 cells co-cultured with amoeba. The inhibition of 3-hydroxy fatty acids by aspirin made C. neoformans more susceptible ( p

    Journal: Frontiers in Microbiology

    Article Title: Cryptococcal 3-Hydroxy Fatty Acids Protect Cells Against Amoebal Phagocytosis

    doi: 10.3389/fmicb.2015.01351

    Figure Lengend Snippet: The results of the survival of aspirin-treated C. neoformans UOFS Y-1378 cells and non-treated C. neoformans UOFS Y-1378 cells co-cultured with amoeba. The inhibition of 3-hydroxy fatty acids by aspirin made C. neoformans more susceptible ( p

    Article Snippet: The relative peak areas of 3-hydroxy nonanoic acid (monoisotopic mass: 174.125595 Da, retention time (RT) 3.7 min) were obtained for each sample and the 3-hydroxy nonanoic acid analytical standard in the Qual Browser function of the Xcalibur software package (Thermo-Fisher Ltd.).

    Techniques: Cell Culture, Inhibition

    Estimation of the physiological concentrations of 3-hydroxy nonanoic acid produced by Cryptococcus neoformans UOFS Y-1378. Using a two-point calibration method, we extrapolated the secreted concentration to be in the range: 0.1–0.4 mM. For each sample class, five biological replicates were analyzed and the values are the mean with standard deviation indicated by error bars. C. gattii was spiked with the analytical standard solution at a final concentration of 0.66 mM.

    Journal: Frontiers in Microbiology

    Article Title: Cryptococcal 3-Hydroxy Fatty Acids Protect Cells Against Amoebal Phagocytosis

    doi: 10.3389/fmicb.2015.01351

    Figure Lengend Snippet: Estimation of the physiological concentrations of 3-hydroxy nonanoic acid produced by Cryptococcus neoformans UOFS Y-1378. Using a two-point calibration method, we extrapolated the secreted concentration to be in the range: 0.1–0.4 mM. For each sample class, five biological replicates were analyzed and the values are the mean with standard deviation indicated by error bars. C. gattii was spiked with the analytical standard solution at a final concentration of 0.66 mM.

    Article Snippet: The relative peak areas of 3-hydroxy nonanoic acid (monoisotopic mass: 174.125595 Da, retention time (RT) 3.7 min) were obtained for each sample and the 3-hydroxy nonanoic acid analytical standard in the Qual Browser function of the Xcalibur software package (Thermo-Fisher Ltd.).

    Techniques: Produced, Concentration Assay, Standard Deviation

    The cytotoxicity effect of 3-hydroxy fatty acids on amoebae. 3-Hydroxy nonanoic acid (3-OH C9:0) was shown to be less effective as an anti-amoebae agent (A,B) when compared to nonanoic acid (C9:0) (C) , which yielded a higher percentage reduction in metabolic activity (% RMA) (D) . At the estimated physiological concentration of 0.2 mM, 3-hydroxy nonanoic acid yielded XTT reduction readings that were highly comparable ( p = 0.11) to readings obtained for non-treated amoebae after a 48 h period. The latter suggest that this molecules does not negative affect the growth of amoebae.

    Journal: Frontiers in Microbiology

    Article Title: Cryptococcal 3-Hydroxy Fatty Acids Protect Cells Against Amoebal Phagocytosis

    doi: 10.3389/fmicb.2015.01351

    Figure Lengend Snippet: The cytotoxicity effect of 3-hydroxy fatty acids on amoebae. 3-Hydroxy nonanoic acid (3-OH C9:0) was shown to be less effective as an anti-amoebae agent (A,B) when compared to nonanoic acid (C9:0) (C) , which yielded a higher percentage reduction in metabolic activity (% RMA) (D) . At the estimated physiological concentration of 0.2 mM, 3-hydroxy nonanoic acid yielded XTT reduction readings that were highly comparable ( p = 0.11) to readings obtained for non-treated amoebae after a 48 h period. The latter suggest that this molecules does not negative affect the growth of amoebae.

    Article Snippet: The relative peak areas of 3-hydroxy nonanoic acid (monoisotopic mass: 174.125595 Da, retention time (RT) 3.7 min) were obtained for each sample and the 3-hydroxy nonanoic acid analytical standard in the Qual Browser function of the Xcalibur software package (Thermo-Fisher Ltd.).

    Techniques: Activity Assay, Concentration Assay

    Comparing the quality of a human IgG1 purified by (i) conventional column, (ii) automated tip column and (iii) batch mode. (a) Analytical SEC profiles of purified IgG1. (b) Reduced SDS-PAGE, M: Mark 12 standard. (c) Non-reduced SDS-PAGE. (d) LC-MS intact mass analysis of light chain. (e) LC-MS intact mass analysis of heavy chain. No significant difference in purity or measured masses was observed in purified IgG from automated processes compared to a conventional column process. Glycosylation variants abbreviations: Man5 (Man 5 GlcNAc 2 ), G0F (GlcNAc 2 Man 3 GlcNAc 2 ), G1F (GalGlcNAc 2 Man 3 (Fuc)GlcNAc 2 ), where: Man (mannose), GlcNAc (N-acetylglucosamine), Gal (Galactose), Fuc (Fucose).

    Journal: mAbs

    Article Title: Automated high throughput microscale antibody purification workflows for accelerating antibody discovery

    doi: 10.1080/19420862.2018.1445450

    Figure Lengend Snippet: Comparing the quality of a human IgG1 purified by (i) conventional column, (ii) automated tip column and (iii) batch mode. (a) Analytical SEC profiles of purified IgG1. (b) Reduced SDS-PAGE, M: Mark 12 standard. (c) Non-reduced SDS-PAGE. (d) LC-MS intact mass analysis of light chain. (e) LC-MS intact mass analysis of heavy chain. No significant difference in purity or measured masses was observed in purified IgG from automated processes compared to a conventional column process. Glycosylation variants abbreviations: Man5 (Man 5 GlcNAc 2 ), G0F (GlcNAc 2 Man 3 GlcNAc 2 ), G1F (GalGlcNAc 2 Man 3 (Fuc)GlcNAc 2 ), where: Man (mannose), GlcNAc (N-acetylglucosamine), Gal (Galactose), Fuc (Fucose).

    Article Snippet: Analytical Size Exclusion Chromatography and SDS-PAGE evaluation of purified IgG Analytical SEC was carried out on an HPLC (Ultimate 3000, Thermo Fisher Scientific) with 214 nm UV monitoring.

    Techniques: Purification, Size-exclusion Chromatography, SDS Page, Liquid Chromatography with Mass Spectroscopy