ampure xp beads system  (Beckman Coulter)


Bioz Verified Symbol Beckman Coulter is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 97

    Structured Review

    Beckman Coulter ampure xp beads system
    Ampure Xp Beads System, supplied by Beckman Coulter, used in various techniques. Bioz Stars score: 97/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ampure xp beads system/product/Beckman Coulter
    Average 97 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    ampure xp beads system - by Bioz Stars, 2020-01
    97/100 stars

    Images

    Related Articles

    Amplification:

    Article Title: Clock-dependent chromatin topology modulates circadian transcription and behavior
    Article Snippet: .. PCR products were purified with the AMPure XP beads system (Beckman Coulter), and amplification profiles were analyzed by fragment analyzer and then sequenced on Illumina HiSeq 2000 machines using single-end 100-bp read length. .. Six-hundred nanograms of 4C template was used for PCR amplification using Sigma-Aldrich long-template PCR system with two-step PCR system from Illumina.

    Article Title: Clock-dependent chromatin topology modulates circadian transcription and behavior
    Article Snippet: Six-hundred nanograms of 4C template was used for PCR amplification using Sigma-Aldrich long-template PCR system with two-step PCR system from Illumina. .. PCR products were purified with the AMPure XP beads system (Beckman Coulter) and then sequenced on NextSeq 500 machines using single-end 75-bp read length.

    RNA Sequencing Assay:

    Article Title: Sublethal effects of imidacloprid on targeting muscle and ribosomal protein related genes in the honey bee Apis mellifera L.
    Article Snippet: Only RNA samples which passed the quality tests were used for RNA-Seq analyses. mRNA was enriched using magnetic beads with Oligo (dT), and then fragmented in 1× NEB Next Magnesium RNA Fragmentation Buffer. .. The AMPure XP beads system (Beckman Coulter, Beverly, USA) was used to select cDNA fragments.

    Article Title: Transcriptomic analysis reveals the roles of microtubule-related genes and transcription factors in fruit length regulation in cucumber (Cucumis sativus L.)
    Article Snippet: Paragraph title: RNA-Seq library construction and sequencing ... Double-stranded cDNAs were synthesized using random hexamers and M-MuLV Reverse Transcriptase (RNase H-), followed by DNA Polymerase I and RNase H. After adenylation of the 3′ ends of cDNA fragments, NEBNext adapter oligonucleotides were ligated to cDNA fragments, and the AMPure XP beads system (Beckman Coulter, Beverly, USA) was used to select cDNA fragments of approximately 200 bp in length. cDNA fragments with ligated adapter molecules on both ends were selectively enriched using the NEB Universal PCR Primer and Index primer in a 10-cycle PCR reaction.

    Magnetic Beads:

    Article Title: Sublethal effects of imidacloprid on targeting muscle and ribosomal protein related genes in the honey bee Apis mellifera L.
    Article Snippet: Only RNA samples which passed the quality tests were used for RNA-Seq analyses. mRNA was enriched using magnetic beads with Oligo (dT), and then fragmented in 1× NEB Next Magnesium RNA Fragmentation Buffer. .. The AMPure XP beads system (Beckman Coulter, Beverly, USA) was used to select cDNA fragments.

    Article Title: Transcriptomic analysis reveals the roles of microtubule-related genes and transcription factors in fruit length regulation in cucumber (Cucumis sativus L.)
    Article Snippet: Briefly, mRNAs were enriched from 3 ug total RNA using magnetic beads with Oligo (dT) (Life technologies, CA, USA), and then fragmented using divalent cations under elevated temperature in the NEB proprietary fragmentation buffer. .. Double-stranded cDNAs were synthesized using random hexamers and M-MuLV Reverse Transcriptase (RNase H-), followed by DNA Polymerase I and RNase H. After adenylation of the 3′ ends of cDNA fragments, NEBNext adapter oligonucleotides were ligated to cDNA fragments, and the AMPure XP beads system (Beckman Coulter, Beverly, USA) was used to select cDNA fragments of approximately 200 bp in length. cDNA fragments with ligated adapter molecules on both ends were selectively enriched using the NEB Universal PCR Primer and Index primer in a 10-cycle PCR reaction.

    Article Title: Transcriptome Profile of the Green Odorous Frog (Odorrana margaretae)
    Article Snippet: The mRNA was purified from total RNA using poly-T oligo-attached magnetic beads (Life Technologies). .. The second cDNA strand synthesis was subsequently performed using DNA Polymerase I and RNase H. The cDNA with an insert size of 200 bp were preferentially purified with AMPure XP beads system (Beckman Coulter) and sequenced on an Illumina HiSeq 2000 platform.

    Article Title: Transcriptomic Profiles Reveal the Interactions of Cd/Zn in Dwarf Polish Wheat (Triticum polonicum L.) Roots
    Article Snippet: Library construction and sequencing mRNA was purified from total RNA using poly-T oligo-attached magnetic beads (Life Technologies, USA) and transcribed to cDNA using random oligonucleotides and M-MuLV Reverse Transcriptase (RNase H− ) (TaKaRa, Dalian, China). .. Then, 200-bp cDNA fragments were purified using the AMPure XP beads system (Beckman Coulter, USA).

    Isolation:

    Article Title: Sublethal effects of imidacloprid on targeting muscle and ribosomal protein related genes in the honey bee Apis mellifera L.
    Article Snippet: Paragraph title: RNA isolation and sequencing ... The AMPure XP beads system (Beckman Coulter, Beverly, USA) was used to select cDNA fragments.

    Synthesized:

    Article Title: Sublethal effects of imidacloprid on targeting muscle and ribosomal protein related genes in the honey bee Apis mellifera L.
    Article Snippet: Next the second-strand cDNA was synthesized using dNTPs, DNA polymerase I and RNAaseH. .. The AMPure XP beads system (Beckman Coulter, Beverly, USA) was used to select cDNA fragments.

    Article Title: Transcriptomic analysis reveals the roles of microtubule-related genes and transcription factors in fruit length regulation in cucumber (Cucumis sativus L.)
    Article Snippet: .. Double-stranded cDNAs were synthesized using random hexamers and M-MuLV Reverse Transcriptase (RNase H-), followed by DNA Polymerase I and RNase H. After adenylation of the 3′ ends of cDNA fragments, NEBNext adapter oligonucleotides were ligated to cDNA fragments, and the AMPure XP beads system (Beckman Coulter, Beverly, USA) was used to select cDNA fragments of approximately 200 bp in length. cDNA fragments with ligated adapter molecules on both ends were selectively enriched using the NEB Universal PCR Primer and Index primer in a 10-cycle PCR reaction. .. Products were purified with the AMPure XP beads system and quantified using the Agilent Bioanalyzer 2100 system.

    Article Title: Transcriptome Profile of the Green Odorous Frog (Odorrana margaretae)
    Article Snippet: The first cDNA strand was synthesized using random oligonucleotides and M-MuLV Reverse Transcriptase (RNase H-). .. The second cDNA strand synthesis was subsequently performed using DNA Polymerase I and RNase H. The cDNA with an insert size of 200 bp were preferentially purified with AMPure XP beads system (Beckman Coulter) and sequenced on an Illumina HiSeq 2000 platform.

    Size-exclusion Chromatography:

    Article Title: Clock-dependent chromatin topology modulates circadian transcription and behavior
    Article Snippet: Six-hundred nanograms of 4C template was used for PCR amplification using Sigma-Aldrich long-template PCR system with bait-specific inverse primers conjugated to Illumina sequencing adaptors (primer sequences are in ) in a final volume of 50 µL in the following PCR program: 2 min at 94°C followed by 30 cycles of 15 sec at 94°C, 1 min at 55°C, and 3 min at 68°C and a final extension of 7 min at 68°C. .. PCR products were purified with the AMPure XP beads system (Beckman Coulter), and amplification profiles were analyzed by fragment analyzer and then sequenced on Illumina HiSeq 2000 machines using single-end 100-bp read length.

    Article Title: Clock-dependent chromatin topology modulates circadian transcription and behavior
    Article Snippet: Purified products were pooled and used as the template of a second PCR reaction with Nextera XT index kit version2 primers (FC-131-2004) in a final volume of 50 µL with the following program: 2 min at 94°C followed by 10 cycles of 15 sec at 94°C, 1 min at 55°C, and 3 min at 68°C and a final extension of 7 min at 68°C. .. PCR products were purified with the AMPure XP beads system (Beckman Coulter) and then sequenced on NextSeq 500 machines using single-end 75-bp read length.

    Article Title: Clock-dependent chromatin topology modulates circadian transcription and behavior
    Article Snippet: Bait-specific inverse primers conjugated to Illumina sequencing adaptors (primer sequences are in the ) were used in a first PCR reaction in a final volume of 50 µL with the following program: 2 min at 94°C followed by 20 cycles of 15 sec at 94°C, 1 min at 55°C, and 3 min at 68°C and a final extension of 7 min at 68°C. .. PCR products were purified with the AMPure XP beads system (Beckman Coulter).

    Construct:

    Article Title: Transcriptome Profile of the Green Odorous Frog (Odorrana margaretae)
    Article Snippet: A single cDNA library was constructed. .. The second cDNA strand synthesis was subsequently performed using DNA Polymerase I and RNase H. The cDNA with an insert size of 200 bp were preferentially purified with AMPure XP beads system (Beckman Coulter) and sequenced on an Illumina HiSeq 2000 platform.

    Purification:

    Article Title: Sublethal effects of imidacloprid on targeting muscle and ribosomal protein related genes in the honey bee Apis mellifera L.
    Article Snippet: The double-strand cDNA was purified by QIAQuick PCR kit. cDNA was then used for end reparation, “A” base addition and ligated with sequencing adapters. .. The AMPure XP beads system (Beckman Coulter, Beverly, USA) was used to select cDNA fragments.

    Article Title: Transcriptomic analysis reveals the roles of microtubule-related genes and transcription factors in fruit length regulation in cucumber (Cucumis sativus L.)
    Article Snippet: Double-stranded cDNAs were synthesized using random hexamers and M-MuLV Reverse Transcriptase (RNase H-), followed by DNA Polymerase I and RNase H. After adenylation of the 3′ ends of cDNA fragments, NEBNext adapter oligonucleotides were ligated to cDNA fragments, and the AMPure XP beads system (Beckman Coulter, Beverly, USA) was used to select cDNA fragments of approximately 200 bp in length. cDNA fragments with ligated adapter molecules on both ends were selectively enriched using the NEB Universal PCR Primer and Index primer in a 10-cycle PCR reaction. .. Products were purified with the AMPure XP beads system and quantified using the Agilent Bioanalyzer 2100 system.

    Article Title: Transcriptome Profile of the Green Odorous Frog (Odorrana margaretae)
    Article Snippet: .. The second cDNA strand synthesis was subsequently performed using DNA Polymerase I and RNase H. The cDNA with an insert size of 200 bp were preferentially purified with AMPure XP beads system (Beckman Coulter) and sequenced on an Illumina HiSeq 2000 platform. .. 101 bp paired-end reads were generated and all raw sequence read data were stored in FastQ format.

    Article Title: Clock-dependent chromatin topology modulates circadian transcription and behavior
    Article Snippet: .. PCR products were purified with the AMPure XP beads system (Beckman Coulter), and amplification profiles were analyzed by fragment analyzer and then sequenced on Illumina HiSeq 2000 machines using single-end 100-bp read length. .. Six-hundred nanograms of 4C template was used for PCR amplification using Sigma-Aldrich long-template PCR system with two-step PCR system from Illumina.

    Article Title: Clock-dependent chromatin topology modulates circadian transcription and behavior
    Article Snippet: .. PCR products were purified with the AMPure XP beads system (Beckman Coulter) and then sequenced on NextSeq 500 machines using single-end 75-bp read length. ..

    Article Title: Clock-dependent chromatin topology modulates circadian transcription and behavior
    Article Snippet: .. PCR products were purified with the AMPure XP beads system (Beckman Coulter). .. Purified products were pooled and used as the template of a second PCR reaction with Nextera XT index kit version2 primers (FC-131-2004) in a final volume of 50 µL with the following program: 2 min at 94°C followed by 10 cycles of 15 sec at 94°C, 1 min at 55°C, and 3 min at 68°C and a final extension of 7 min at 68°C.

    Article Title: Transcriptomic Profiles Reveal the Interactions of Cd/Zn in Dwarf Polish Wheat (Triticum polonicum L.) Roots
    Article Snippet: .. Then, 200-bp cDNA fragments were purified using the AMPure XP beads system (Beckman Coulter, USA). .. Ten cycles of PCR amplifications were performed to enrich cDNA fragments using the NEB Universal PCR primer and Index primer (Illumia, USA).

    Sequencing:

    Article Title: Sublethal effects of imidacloprid on targeting muscle and ribosomal protein related genes in the honey bee Apis mellifera L.
    Article Snippet: Paragraph title: RNA isolation and sequencing ... The AMPure XP beads system (Beckman Coulter, Beverly, USA) was used to select cDNA fragments.

    Article Title: Transcriptomic analysis reveals the roles of microtubule-related genes and transcription factors in fruit length regulation in cucumber (Cucumis sativus L.)
    Article Snippet: Paragraph title: RNA-Seq library construction and sequencing ... Double-stranded cDNAs were synthesized using random hexamers and M-MuLV Reverse Transcriptase (RNase H-), followed by DNA Polymerase I and RNase H. After adenylation of the 3′ ends of cDNA fragments, NEBNext adapter oligonucleotides were ligated to cDNA fragments, and the AMPure XP beads system (Beckman Coulter, Beverly, USA) was used to select cDNA fragments of approximately 200 bp in length. cDNA fragments with ligated adapter molecules on both ends were selectively enriched using the NEB Universal PCR Primer and Index primer in a 10-cycle PCR reaction.

    Article Title: Transcriptome Profile of the Green Odorous Frog (Odorrana margaretae)
    Article Snippet: Paragraph title: cDNA library construction and Illumina sequencing ... The second cDNA strand synthesis was subsequently performed using DNA Polymerase I and RNase H. The cDNA with an insert size of 200 bp were preferentially purified with AMPure XP beads system (Beckman Coulter) and sequenced on an Illumina HiSeq 2000 platform.

    Article Title: Clock-dependent chromatin topology modulates circadian transcription and behavior
    Article Snippet: Paragraph title: Inverse PCR and sequencing in wild-type and Bmal1 knockout mouse livers and kidneys ... PCR products were purified with the AMPure XP beads system (Beckman Coulter), and amplification profiles were analyzed by fragment analyzer and then sequenced on Illumina HiSeq 2000 machines using single-end 100-bp read length.

    Article Title: Transcriptomic Profiles Reveal the Interactions of Cd/Zn in Dwarf Polish Wheat (Triticum polonicum L.) Roots
    Article Snippet: Paragraph title: Library construction and sequencing ... Then, 200-bp cDNA fragments were purified using the AMPure XP beads system (Beckman Coulter, USA).

    cDNA Library Assay:

    Article Title: Transcriptome Profile of the Green Odorous Frog (Odorrana margaretae)
    Article Snippet: Paragraph title: cDNA library construction and Illumina sequencing ... The second cDNA strand synthesis was subsequently performed using DNA Polymerase I and RNase H. The cDNA with an insert size of 200 bp were preferentially purified with AMPure XP beads system (Beckman Coulter) and sequenced on an Illumina HiSeq 2000 platform.

    Generated:

    Article Title: Transcriptome Profile of the Green Odorous Frog (Odorrana margaretae)
    Article Snippet: The second cDNA strand synthesis was subsequently performed using DNA Polymerase I and RNase H. The cDNA with an insert size of 200 bp were preferentially purified with AMPure XP beads system (Beckman Coulter) and sequenced on an Illumina HiSeq 2000 platform. .. 101 bp paired-end reads were generated and all raw sequence read data were stored in FastQ format.

    Knock-Out:

    Article Title: Clock-dependent chromatin topology modulates circadian transcription and behavior
    Article Snippet: Paragraph title: Inverse PCR and sequencing in wild-type and Bmal1 knockout mouse livers and kidneys ... PCR products were purified with the AMPure XP beads system (Beckman Coulter), and amplification profiles were analyzed by fragment analyzer and then sequenced on Illumina HiSeq 2000 machines using single-end 100-bp read length.

    Polymerase Chain Reaction:

    Article Title: Sublethal effects of imidacloprid on targeting muscle and ribosomal protein related genes in the honey bee Apis mellifera L.
    Article Snippet: The double-strand cDNA was purified by QIAQuick PCR kit. cDNA was then used for end reparation, “A” base addition and ligated with sequencing adapters. .. The AMPure XP beads system (Beckman Coulter, Beverly, USA) was used to select cDNA fragments.

    Article Title: Transcriptomic analysis reveals the roles of microtubule-related genes and transcription factors in fruit length regulation in cucumber (Cucumis sativus L.)
    Article Snippet: .. Double-stranded cDNAs were synthesized using random hexamers and M-MuLV Reverse Transcriptase (RNase H-), followed by DNA Polymerase I and RNase H. After adenylation of the 3′ ends of cDNA fragments, NEBNext adapter oligonucleotides were ligated to cDNA fragments, and the AMPure XP beads system (Beckman Coulter, Beverly, USA) was used to select cDNA fragments of approximately 200 bp in length. cDNA fragments with ligated adapter molecules on both ends were selectively enriched using the NEB Universal PCR Primer and Index primer in a 10-cycle PCR reaction. .. Products were purified with the AMPure XP beads system and quantified using the Agilent Bioanalyzer 2100 system.

    Article Title: Clock-dependent chromatin topology modulates circadian transcription and behavior
    Article Snippet: .. PCR products were purified with the AMPure XP beads system (Beckman Coulter), and amplification profiles were analyzed by fragment analyzer and then sequenced on Illumina HiSeq 2000 machines using single-end 100-bp read length. .. Six-hundred nanograms of 4C template was used for PCR amplification using Sigma-Aldrich long-template PCR system with two-step PCR system from Illumina.

    Article Title: Clock-dependent chromatin topology modulates circadian transcription and behavior
    Article Snippet: .. PCR products were purified with the AMPure XP beads system (Beckman Coulter) and then sequenced on NextSeq 500 machines using single-end 75-bp read length. ..

    Article Title: Clock-dependent chromatin topology modulates circadian transcription and behavior
    Article Snippet: .. PCR products were purified with the AMPure XP beads system (Beckman Coulter). .. Purified products were pooled and used as the template of a second PCR reaction with Nextera XT index kit version2 primers (FC-131-2004) in a final volume of 50 µL with the following program: 2 min at 94°C followed by 10 cycles of 15 sec at 94°C, 1 min at 55°C, and 3 min at 68°C and a final extension of 7 min at 68°C.

    Article Title: Transcriptomic Profiles Reveal the Interactions of Cd/Zn in Dwarf Polish Wheat (Triticum polonicum L.) Roots
    Article Snippet: Then, 200-bp cDNA fragments were purified using the AMPure XP beads system (Beckman Coulter, USA). .. Ten cycles of PCR amplifications were performed to enrich cDNA fragments using the NEB Universal PCR primer and Index primer (Illumia, USA).

    Inverse PCR:

    Article Title: Clock-dependent chromatin topology modulates circadian transcription and behavior
    Article Snippet: Paragraph title: Inverse PCR and sequencing in wild-type and Bmal1 knockout mouse livers and kidneys ... PCR products were purified with the AMPure XP beads system (Beckman Coulter), and amplification profiles were analyzed by fragment analyzer and then sequenced on Illumina HiSeq 2000 machines using single-end 100-bp read length.

    Concentration Assay:

    Article Title: Sublethal effects of imidacloprid on targeting muscle and ribosomal protein related genes in the honey bee Apis mellifera L.
    Article Snippet: RNA purity was determined using the NanoDrop 2000 and the RNA integrity and concentration were evaluated by Agilent 2100 RNA Nano 6000 Assay Kit (Agilent Technologies, Inc., Santa Clara, CA, USA). .. The AMPure XP beads system (Beckman Coulter, Beverly, USA) was used to select cDNA fragments.

    Article Title: Transcriptome Profile of the Green Odorous Frog (Odorrana margaretae)
    Article Snippet: RNA integrity was assessed using the RNA 6000 Nano Assay Kit with a Bioanalyzer 2100 (Agilent Technologies) after checking the RNA purity and concentration. .. The second cDNA strand synthesis was subsequently performed using DNA Polymerase I and RNase H. The cDNA with an insert size of 200 bp were preferentially purified with AMPure XP beads system (Beckman Coulter) and sequenced on an Illumina HiSeq 2000 platform.

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 78
    Beckman Coulter magnetic beads nucleic acid extraction agencout ampure xp pcr purification kit
    Cellulose dipsticks outperform a commercially available nucleic acid purification system. (A) The time required, number of pipetting steps involved, and the costs of all consumables—including tubes and pipette tips—were calculated for purification of nucleic acids from Arabidopsis leaf tissue using either the cellulose dipstick or Agencourt <t>AMPure</t> paramagnetic beads. All solutions that could be prepared in advance, including lysis and wash buffers, were made and prealiquoted. The time and pipetting involved in the preparation of these solutions was not added to the tallies in the table. (B) Purified Arabidopsis DNA at different concentrations was a captured, washed, and eluted using either the cellulose dipsticks or AMPure paramagnetic beads (Beckman Coulter). The eluted DNA was used in a <t>PCR</t> reaction with using primers designed for the G-protein gamma subunit 1 gene. The band intensities relative to the 1 ng/μl paramagnetic bead sample appear below each band. (C) Different volumes of an Arabidopsis leaf extract were captured, washed, and eluted using either the cellulose dipsticks or AMPure paramagnetic beads and subsequently amplified in a PCR reaction as described above. The band intensities relative to the 50 μl paramagnetic bead sample appear below each band. n.a., no amplification; USD, United States Dollar.
    Magnetic Beads Nucleic Acid Extraction Agencout Ampure Xp Pcr Purification Kit, supplied by Beckman Coulter, used in various techniques. Bioz Stars score: 78/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/magnetic beads nucleic acid extraction agencout ampure xp pcr purification kit/product/Beckman Coulter
    Average 78 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    magnetic beads nucleic acid extraction agencout ampure xp pcr purification kit - by Bioz Stars, 2020-01
    78/100 stars
      Buy from Supplier

    99
    Beckman Coulter agencourt ampure xp beads
    Drosophila sequencing library. a and b Agarose gel (2%) demonstrating the PCR products from surface tagmentation, various amount of acrydite oligonucleotides were used when casting the poly-acrylamide gel. c Agarose gel (2%) demonstrating the sequencing library after surface tagmentation and 16 cycles of PCR. The sequencing library was size selected with <t>Agencourt</t> <t>Ampure</t> XP beads
    Agencourt Ampure Xp Beads, supplied by Beckman Coulter, used in various techniques. Bioz Stars score: 99/100, based on 3026 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/agencourt ampure xp beads/product/Beckman Coulter
    Average 99 stars, based on 3026 article reviews
    Price from $9.99 to $1999.99
    agencourt ampure xp beads - by Bioz Stars, 2020-01
    99/100 stars
      Buy from Supplier

    Image Search Results


    Cellulose dipsticks outperform a commercially available nucleic acid purification system. (A) The time required, number of pipetting steps involved, and the costs of all consumables—including tubes and pipette tips—were calculated for purification of nucleic acids from Arabidopsis leaf tissue using either the cellulose dipstick or Agencourt AMPure paramagnetic beads. All solutions that could be prepared in advance, including lysis and wash buffers, were made and prealiquoted. The time and pipetting involved in the preparation of these solutions was not added to the tallies in the table. (B) Purified Arabidopsis DNA at different concentrations was a captured, washed, and eluted using either the cellulose dipsticks or AMPure paramagnetic beads (Beckman Coulter). The eluted DNA was used in a PCR reaction with using primers designed for the G-protein gamma subunit 1 gene. The band intensities relative to the 1 ng/μl paramagnetic bead sample appear below each band. (C) Different volumes of an Arabidopsis leaf extract were captured, washed, and eluted using either the cellulose dipsticks or AMPure paramagnetic beads and subsequently amplified in a PCR reaction as described above. The band intensities relative to the 50 μl paramagnetic bead sample appear below each band. n.a., no amplification; USD, United States Dollar.

    Journal: PLoS Biology

    Article Title: Nucleic acid purification from plants, animals and microbes in under 30 seconds

    doi: 10.1371/journal.pbio.2003916

    Figure Lengend Snippet: Cellulose dipsticks outperform a commercially available nucleic acid purification system. (A) The time required, number of pipetting steps involved, and the costs of all consumables—including tubes and pipette tips—were calculated for purification of nucleic acids from Arabidopsis leaf tissue using either the cellulose dipstick or Agencourt AMPure paramagnetic beads. All solutions that could be prepared in advance, including lysis and wash buffers, were made and prealiquoted. The time and pipetting involved in the preparation of these solutions was not added to the tallies in the table. (B) Purified Arabidopsis DNA at different concentrations was a captured, washed, and eluted using either the cellulose dipsticks or AMPure paramagnetic beads (Beckman Coulter). The eluted DNA was used in a PCR reaction with using primers designed for the G-protein gamma subunit 1 gene. The band intensities relative to the 1 ng/μl paramagnetic bead sample appear below each band. (C) Different volumes of an Arabidopsis leaf extract were captured, washed, and eluted using either the cellulose dipsticks or AMPure paramagnetic beads and subsequently amplified in a PCR reaction as described above. The band intensities relative to the 50 μl paramagnetic bead sample appear below each band. n.a., no amplification; USD, United States Dollar.

    Article Snippet: Magnetic beads nucleic acid extraction Agencout AMPure XP PCR Purification kit (Beckman Coulter) was used to purify DNA following the manufacturer’s recommendations.

    Techniques: Nucleic Acid Purification, Transferring, Purification, Lysis, Polymerase Chain Reaction, Amplification

    Drosophila sequencing library. a and b Agarose gel (2%) demonstrating the PCR products from surface tagmentation, various amount of acrydite oligonucleotides were used when casting the poly-acrylamide gel. c Agarose gel (2%) demonstrating the sequencing library after surface tagmentation and 16 cycles of PCR. The sequencing library was size selected with Agencourt Ampure XP beads

    Journal: BMC Genomics

    Article Title: Next-generation sequencing library construction on a surface

    doi: 10.1186/s12864-018-4797-4

    Figure Lengend Snippet: Drosophila sequencing library. a and b Agarose gel (2%) demonstrating the PCR products from surface tagmentation, various amount of acrydite oligonucleotides were used when casting the poly-acrylamide gel. c Agarose gel (2%) demonstrating the sequencing library after surface tagmentation and 16 cycles of PCR. The sequencing library was size selected with Agencourt Ampure XP beads

    Article Snippet: The PCR products were purified and size selected by Agencourt AMPure XP beads (Beckman Coulter).

    Techniques: Sequencing, Agarose Gel Electrophoresis, Polymerase Chain Reaction, Acrylamide Gel Assay

    Drosophila sequencing library. a and b Agarose gel (2%) demonstrating the PCR products from surface tagmentation, various amount of acrydite oligonucleotides were used when casting the poly-acrylamide gel. c Agarose gel (2%) demonstrating the sequencing library after surface tagmentation and 16 cycles of PCR. The sequencing library was size selected with Agencourt Ampure XP beads

    Journal: BMC Genomics

    Article Title: Next-generation sequencing library construction on a surface

    doi: 10.1186/s12864-018-4797-4

    Figure Lengend Snippet: Drosophila sequencing library. a and b Agarose gel (2%) demonstrating the PCR products from surface tagmentation, various amount of acrydite oligonucleotides were used when casting the poly-acrylamide gel. c Agarose gel (2%) demonstrating the sequencing library after surface tagmentation and 16 cycles of PCR. The sequencing library was size selected with Agencourt Ampure XP beads

    Article Snippet: The PCR products were purified and size selected by Agencourt AMPure XP beads (Beckman Coulter).

    Techniques: Sequencing, Agarose Gel Electrophoresis, Polymerase Chain Reaction, Acrylamide Gel Assay

    cIAP1 is required for the recruitment of E2F1 onto chromatin. ChIP-seq analysis of the recruitment of E2F1 onto chromatin in HeLa cells transfected with scrambled (Sc) or cIAP1-siRNA. Enriched DNA from ChIP and Input DNA fragments were end-repaired, extended with an ‘A’ base on the 3′end, ligated with indexed paired-end adaptors (NEXTflex, Bio Scientific) using the Bravo Platform (Agilent), size-selected after 4 cycles of PCR with AMPure XP beads (Beckman Coulter) and amplified by PCR for 10 more cycles (A). The efficiency of siRNA was checked by Western Blot analysis (B).

    Journal: PLoS ONE

    Article Title: E2F1 binds to the peptide-binding groove within the BIR3 domain of cIAP1 and requires cIAP1 for chromatin binding

    doi: 10.1371/journal.pone.0206253

    Figure Lengend Snippet: cIAP1 is required for the recruitment of E2F1 onto chromatin. ChIP-seq analysis of the recruitment of E2F1 onto chromatin in HeLa cells transfected with scrambled (Sc) or cIAP1-siRNA. Enriched DNA from ChIP and Input DNA fragments were end-repaired, extended with an ‘A’ base on the 3′end, ligated with indexed paired-end adaptors (NEXTflex, Bio Scientific) using the Bravo Platform (Agilent), size-selected after 4 cycles of PCR with AMPure XP beads (Beckman Coulter) and amplified by PCR for 10 more cycles (A). The efficiency of siRNA was checked by Western Blot analysis (B).

    Article Snippet: For the ChIP-seq analysis, enriched DNA from ChIP and input DNA fragments were end-repaired, extended with an ‘A’ base on the 3′-end, ligated with indexed paired-end adaptors (NEXTflex, Bio Scientific, Saint-Marcel, France) using the Bravo Platform (Agilent), size-selected after 4 cycles of PCR with AMPure XP beads (Beckman Coulter, Villepinte, France) and amplified by PCR for 10 more cycles.

    Techniques: Chromatin Immunoprecipitation, Transfection, Polymerase Chain Reaction, Amplification, Western Blot