amplitaq gold dna polymerase  (Thermo Fisher)


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    Name:
    AmpliTaq Gold DNA Polymerase
    Description:
    AmpliTaq Gold DNA Polymerase is a chemically modified form of AmpliTaq DNA Polymerase requiring thermal activation Features of this enzyme • Automated chemical hot start enzyme for increased specificity sensitivity and yield• Time released thermal activation improves sensitivity in low copy number amplifications• Successful multiplex PCR saves time and reagents• Includes GeneAmp 10X PCR Gold Buffer with MgCl2Hot start activationThe modified enzyme is provided in an inactive state Upon thermal activation the modifier is permanently released regenerating active enzyme The resulting hot start PCR amplification provides increased sensitivity specificity and yield over conventional PCR techniques Higher specificity higher yieldAmpliTaq Gold DNA Polymerase can be activated partially or completely in a pre PCR heat step or can be allowed to activate slowly in a time released manner during the denaturation steps of thermal cycling With or without a limited up front heat activation step active enzyme is released slowly during thermal cycling to match template concentration and increase specificity The yield of specific product increases because reactants are not wasted in the formation of unintended products Because AmpliTaq Gold DNA Polymerase is a chemical hot start enzyme there is no worry of biological contamination GeneAmp 10X PCR Gold Buffer is formulated to provide flexible efficient activation of AmpliTaq Gold DNA Polymerase resulting in a highly specific robust PCR amplification The ionic strength and the pH of GeneAmp 10X PCR Gold Buffer have been optimized to provide a wider activation temperature and time range when used in conjunction with AmpliTaq Gold DNA Polymerase AmpliTaq Gold 360 DNA Polymerase for superior performanceAlso available is AmpliTaq Gold 360 DNA Polymerase for even better PCR performance Compared to AmpliTaq Gold DNA Polymerase AmpliTaq Gold 360 DNA Polymerase is purified by an additional proprietary separation process to eliminate contaminating bacterial DNA sequences from the enzyme preparation When used with the enhanced 360 Gold Buffer this ultra pure enzyme in addition to its hot start capabilities reduces false positive results amplifies low level target sequences and promotes the amplification of a variety of templates Also included with this product is a 360 GC Enhancer to work through challenging GC rich templates
    Catalog Number:
    4311806
    Price:
    None
    Category:
    Proteins Enzymes Peptides
    Applications:
    Hot Start PCR|PCR|PCR & Real-Time PCR|PCR Enzymes & Master Mixes
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    Structured Review

    Thermo Fisher amplitaq gold dna polymerase
    AmpliTaq Gold DNA Polymerase is a chemically modified form of AmpliTaq DNA Polymerase requiring thermal activation Features of this enzyme • Automated chemical hot start enzyme for increased specificity sensitivity and yield• Time released thermal activation improves sensitivity in low copy number amplifications• Successful multiplex PCR saves time and reagents• Includes GeneAmp 10X PCR Gold Buffer with MgCl2Hot start activationThe modified enzyme is provided in an inactive state Upon thermal activation the modifier is permanently released regenerating active enzyme The resulting hot start PCR amplification provides increased sensitivity specificity and yield over conventional PCR techniques Higher specificity higher yieldAmpliTaq Gold DNA Polymerase can be activated partially or completely in a pre PCR heat step or can be allowed to activate slowly in a time released manner during the denaturation steps of thermal cycling With or without a limited up front heat activation step active enzyme is released slowly during thermal cycling to match template concentration and increase specificity The yield of specific product increases because reactants are not wasted in the formation of unintended products Because AmpliTaq Gold DNA Polymerase is a chemical hot start enzyme there is no worry of biological contamination GeneAmp 10X PCR Gold Buffer is formulated to provide flexible efficient activation of AmpliTaq Gold DNA Polymerase resulting in a highly specific robust PCR amplification The ionic strength and the pH of GeneAmp 10X PCR Gold Buffer have been optimized to provide a wider activation temperature and time range when used in conjunction with AmpliTaq Gold DNA Polymerase AmpliTaq Gold 360 DNA Polymerase for superior performanceAlso available is AmpliTaq Gold 360 DNA Polymerase for even better PCR performance Compared to AmpliTaq Gold DNA Polymerase AmpliTaq Gold 360 DNA Polymerase is purified by an additional proprietary separation process to eliminate contaminating bacterial DNA sequences from the enzyme preparation When used with the enhanced 360 Gold Buffer this ultra pure enzyme in addition to its hot start capabilities reduces false positive results amplifies low level target sequences and promotes the amplification of a variety of templates Also included with this product is a 360 GC Enhancer to work through challenging GC rich templates
    https://www.bioz.com/result/amplitaq gold dna polymerase/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    amplitaq gold dna polymerase - by Bioz Stars, 2021-03
    99/100 stars

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    Related Articles

    Gel Extraction:

    Article Title: Variants of a Thermus aquaticus DNA Polymerase with Increased Selectivity for Applications in Allele- and Methylation-Specific Amplification
    Article Snippet: .. Reagents and Instruments Oligonucleotides were purchased from Biomers or Metabion, HeLa genomic DNA and Taq 2x master mix was bought from New England Biolabs, dNTPs were either from Roche or Fermentas, Phusion DNA polymerase was purchased from Thermo Scientific, Platinum Taq and AmpliTaq Gold DNA polymerases from Life Technologies, DNase I, SphI, and HindIII from Fermentas, the Gel Extraction and EpiTect MSP kit from Qiagen and used according to their manuals. .. KlenTaq and its respective mutants were recombinantly expressed in E. coli BL21 (DE3) and purified with Ni-IDA as previously described .

    Polymerase Chain Reaction:

    Article Title: 7-Deaza-2`-deoxyguanosine allows PCR and sequencing reactions from CpG islands
    Article Snippet: We amplified the human p16INK4A promoter (accession number ) and HUMARA exon 1 (accession number ) according to previously published methods. .. The PCR mixtures contained 1× PCR buffer (Taq polymerase: 50mM KCl, 100mM Tris/HCl, pH 9.0, 0.1% (vol/vol) Triton-X 100, or AmpliTaq Gold polymerase: PCR Gold buffer (Applied Biosystems, Weiterstadt, Germany)), 1.5mM (p16INK4A ) or 2.0mM (HUMARA) magnesium chloride, 200μM dNTP mix (both) or deaza-mix (deaza-dGTP instead of dGTP), 10 pmol of each primer (p16INK4A WT , HUMARA ), 1 U Taq polymerase (Promega, Heidelberg, Germany) or for hot start PCR AmpliTaq Gold (Applied Biosystems) and 50 ng template (p16INK4A , SW1116; HUMARA, ARH77) in a final volume of 25 μl. .. The following PCR cycle profiles were used. p16INK4A : five minutes (Taq polymerase) or 12 minutes (AmpliTaq Gold) at 94°C; 35 cycles of 20 seconds at 94°C, 20 seconds at 65°C, and 20 seconds at 72°C; followed by two minutes at 72°C.

    Article Title: Selective control of primer usage in multiplex one-step reverse transcription PCR
    Article Snippet: One-step RT-PCR (Endpoint) A one-step RT-PCR protocol was used in which the components were combined in a single tube. .. Reaction conditions included 1× PCR buffer (20 mM Tris (pH 8.4), 50 mM KCl) (Invitrogen), 1.5 mM MgCl2 (Invitrogen), gene-specific PCR primers (0.5 μM) (TriLink), oligo(dT)18 primer (1 μM) (TriLink), 0.16 mM dNTPs (New England Biolabs), 0.5 μL of human trachea total RNA or other human total RNA (Stratagene or Ambion, each at ~1.6 μg/μL), 5 U RNase Inhibitor (New England Biolabs), 50 U Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) (Invitrogen) or SuperScript® III Reverse Transcriptase (SSIII RT) (Invitrogen), and 0.6 U of Taq DNA Polymerase, recombinant (Invitrogen) or Platinum® Taq DNA Polymerase (Invitrogen) or AmpliTaq Gold® DNA Polymerase (Applied Biosystems), in a 50 μL reaction volume. .. Thermal cycling conditions utilized a reverse transcription step at 42°C for 30 min when M-MLV RT was used and 55°C for 30 min when SSIII RT was employed; 95°C for 10 min (RT inactivation and initial denaturation step), followed by 45 PCR cycles at 95°C for 30 sec, 60°C for 1 min and final extension at 72°C for 5 min.

    Article Title: Tagsteady: a metabarcoding library preparation protocol to avoid false assignment of sequences to samples
    Article Snippet: Prior to metabarcoding PCRs, for all three primer sets quantitative PCR was carried out to optimise metabarcoding PCR conditions and assess negative extraction controls as described in . .. The 16sIns PCRs were carried out in 25 μl reactions containing 1 μl template DNA, 1 U AmpliTaq Gold, 1x Gold PCR Buffer and 2.5 mM MgCl2 (all from Applied Biosystems), 0.2 mM dNTP mix (Invitrogen), 0.5 mg/ml BSA, 0.6 μM of each 5’ nucleotide-tagged forward and reverse primer. .. PCR conditions for 16sIns were as follows: 95°C for 10 minutes, 35 cycles of 95°C for 15 seconds, 54°C for 30 seconds and 72°C for 30 seconds and a seven minutes final extension at 72°C.

    Hot Start PCR:

    Article Title: 7-Deaza-2`-deoxyguanosine allows PCR and sequencing reactions from CpG islands
    Article Snippet: We amplified the human p16INK4A promoter (accession number ) and HUMARA exon 1 (accession number ) according to previously published methods. .. The PCR mixtures contained 1× PCR buffer (Taq polymerase: 50mM KCl, 100mM Tris/HCl, pH 9.0, 0.1% (vol/vol) Triton-X 100, or AmpliTaq Gold polymerase: PCR Gold buffer (Applied Biosystems, Weiterstadt, Germany)), 1.5mM (p16INK4A ) or 2.0mM (HUMARA) magnesium chloride, 200μM dNTP mix (both) or deaza-mix (deaza-dGTP instead of dGTP), 10 pmol of each primer (p16INK4A WT , HUMARA ), 1 U Taq polymerase (Promega, Heidelberg, Germany) or for hot start PCR AmpliTaq Gold (Applied Biosystems) and 50 ng template (p16INK4A , SW1116; HUMARA, ARH77) in a final volume of 25 μl. .. The following PCR cycle profiles were used. p16INK4A : five minutes (Taq polymerase) or 12 minutes (AmpliTaq Gold) at 94°C; 35 cycles of 20 seconds at 94°C, 20 seconds at 65°C, and 20 seconds at 72°C; followed by two minutes at 72°C.

    other:

    Article Title: A novel MALDI-TOF based methodology for genotyping single nucleotide polymorphisms
    Article Snippet: AmpliTaq Gold® DNA polymerase was purchased from Applied Biosystems (Foster City, CA).

    Recombinant:

    Article Title: Selective control of primer usage in multiplex one-step reverse transcription PCR
    Article Snippet: One-step RT-PCR (Endpoint) A one-step RT-PCR protocol was used in which the components were combined in a single tube. .. Reaction conditions included 1× PCR buffer (20 mM Tris (pH 8.4), 50 mM KCl) (Invitrogen), 1.5 mM MgCl2 (Invitrogen), gene-specific PCR primers (0.5 μM) (TriLink), oligo(dT)18 primer (1 μM) (TriLink), 0.16 mM dNTPs (New England Biolabs), 0.5 μL of human trachea total RNA or other human total RNA (Stratagene or Ambion, each at ~1.6 μg/μL), 5 U RNase Inhibitor (New England Biolabs), 50 U Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) (Invitrogen) or SuperScript® III Reverse Transcriptase (SSIII RT) (Invitrogen), and 0.6 U of Taq DNA Polymerase, recombinant (Invitrogen) or Platinum® Taq DNA Polymerase (Invitrogen) or AmpliTaq Gold® DNA Polymerase (Applied Biosystems), in a 50 μL reaction volume. .. Thermal cycling conditions utilized a reverse transcription step at 42°C for 30 min when M-MLV RT was used and 55°C for 30 min when SSIII RT was employed; 95°C for 10 min (RT inactivation and initial denaturation step), followed by 45 PCR cycles at 95°C for 30 sec, 60°C for 1 min and final extension at 72°C for 5 min.

    Real-time Polymerase Chain Reaction:

    Article Title: Absence of XMRV Retrovirus and Other Murine Leukemia Virus-Related Viruses in Patients with Chronic Fatigue Syndrome ▿Absence of XMRV Retrovirus and Other Murine Leukemia Virus-Related Viruses in Patients with Chronic Fatigue Syndrome ▿ ¶
    Article Snippet: .. Applied Biosystems' AmpliTaq Gold Taq polymerase contained in the master mix of all of our qPCR TaqMan assays did not contain any IAP sequences ( D), indicating that it was free of mouse DNA. .. When additional AmpliTaq Gold Taq polymerase was added as a template for the IAP qPCR assay, as was done with the other polymerase preparations, all reactions remained negative.

    Ancient DNA Assay:

    Article Title: Patterns of nucleotide misincorporations during enzymatic amplification and direct large-scale sequencing of ancient DNA
    Article Snippet: .. These oligonucleotides were used as templates in PCRs with two DNA polymerase reagents, AmpliTaq Gold (Applied Biosystems, Foster City, CA) and Platinum Taq High Fidelity (Invitrogen, Carlsbad, CA), which are both commonly used in ancient DNA research. .. In contrast, when the oligonucleotide carrying the U was used, both polymerase reagents inserted A residues opposite the template sites carrying a U residue in all clones sequenced (data not shown).

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    Thermo Fisher amplitaq gold dna polymerase
    Hot Start <t>DNA</t> polymerase evaluation in triplex one-step RT-PCR amplification of ABCA5, ABCA6 and ABCA7 targets . Reactions contained 0.82 μg of human trachea total RNA and an unmodified oligo(dT) 18 RT primer. The PCR primers were either unmodified, or contained CleanAmp™ Precision modifications. These reactions contained one of the following DNA polymerases: Taq , Platinum ® Taq , or <t>AmpliTaq</t> Gold ® and one of the following reverse transcriptases: M-MLV or SSIII. Reactions with M-MLV were incubated at 42°C, while reactions with SSIII were incubated at 55°C.
    Amplitaq Gold Dna Polymerase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/amplitaq gold dna polymerase/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    amplitaq gold dna polymerase - by Bioz Stars, 2021-03
    99/100 stars
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    Hot Start DNA polymerase evaluation in triplex one-step RT-PCR amplification of ABCA5, ABCA6 and ABCA7 targets . Reactions contained 0.82 μg of human trachea total RNA and an unmodified oligo(dT) 18 RT primer. The PCR primers were either unmodified, or contained CleanAmp™ Precision modifications. These reactions contained one of the following DNA polymerases: Taq , Platinum ® Taq , or AmpliTaq Gold ® and one of the following reverse transcriptases: M-MLV or SSIII. Reactions with M-MLV were incubated at 42°C, while reactions with SSIII were incubated at 55°C.

    Journal: BMC Molecular Biology

    Article Title: Selective control of primer usage in multiplex one-step reverse transcription PCR

    doi: 10.1186/1471-2199-10-113

    Figure Lengend Snippet: Hot Start DNA polymerase evaluation in triplex one-step RT-PCR amplification of ABCA5, ABCA6 and ABCA7 targets . Reactions contained 0.82 μg of human trachea total RNA and an unmodified oligo(dT) 18 RT primer. The PCR primers were either unmodified, or contained CleanAmp™ Precision modifications. These reactions contained one of the following DNA polymerases: Taq , Platinum ® Taq , or AmpliTaq Gold ® and one of the following reverse transcriptases: M-MLV or SSIII. Reactions with M-MLV were incubated at 42°C, while reactions with SSIII were incubated at 55°C.

    Article Snippet: Reaction conditions included 1× PCR buffer (20 mM Tris (pH 8.4), 50 mM KCl) (Invitrogen), 1.5 mM MgCl2 (Invitrogen), gene-specific PCR primers (0.5 μM) (TriLink), oligo(dT)18 primer (1 μM) (TriLink), 0.16 mM dNTPs (New England Biolabs), 0.5 μL of human trachea total RNA or other human total RNA (Stratagene or Ambion, each at ~1.6 μg/μL), 5 U RNase Inhibitor (New England Biolabs), 50 U Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) (Invitrogen) or SuperScript® III Reverse Transcriptase (SSIII RT) (Invitrogen), and 0.6 U of Taq DNA Polymerase, recombinant (Invitrogen) or Platinum® Taq DNA Polymerase (Invitrogen) or AmpliTaq Gold® DNA Polymerase (Applied Biosystems), in a 50 μL reaction volume.

    Techniques: Reverse Transcription Polymerase Chain Reaction, Amplification, Polymerase Chain Reaction, Incubation

    Summary of the oligonucleotide competition amplifications. Each bar represents the ratio of nucleotides found among clones from amplification products generated from a mixture of two oligonucleotides, given by the abbreviations below the bars. Misincorporated nucleotides are indicated by the black upper part of each bar, and numbers give the numbers of clones sequenced. The polymerase reagents are indicated as “Gold” for AmpliTaq Gold and “Platin” for Platinum Taq High Fidelity.

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Patterns of nucleotide misincorporations during enzymatic amplification and direct large-scale sequencing of ancient DNA

    doi: 10.1073/pnas.0605327103

    Figure Lengend Snippet: Summary of the oligonucleotide competition amplifications. Each bar represents the ratio of nucleotides found among clones from amplification products generated from a mixture of two oligonucleotides, given by the abbreviations below the bars. Misincorporated nucleotides are indicated by the black upper part of each bar, and numbers give the numbers of clones sequenced. The polymerase reagents are indicated as “Gold” for AmpliTaq Gold and “Platin” for Platinum Taq High Fidelity.

    Article Snippet: These oligonucleotides were used as templates in PCRs with two DNA polymerase reagents, AmpliTaq Gold (Applied Biosystems, Foster City, CA) and Platinum Taq High Fidelity (Invitrogen, Carlsbad, CA), which are both commonly used in ancient DNA research.

    Techniques: Clone Assay, Amplification, Generated

    (A) 7-Deaza-2`-deoxyguanosine (deaza-dGTP) allows PCR of the human p16 INK4A promoter because the specific 140 bp product (black arrow, 140) is clearly visible whether standard Taq polymerase (no hot start) or AmpliTaq Gold (hot start) is used. (B) dGTP gives rise to unspecific byproducts (black arrow, by), which are reduced if hot start PCR is performed. The 600 bp band of the 100 bp ladder standard is indicated (white arrow, 600).

    Journal: Molecular Pathology

    Article Title: 7-Deaza-2`-deoxyguanosine allows PCR and sequencing reactions from CpG islands

    doi:

    Figure Lengend Snippet: (A) 7-Deaza-2`-deoxyguanosine (deaza-dGTP) allows PCR of the human p16 INK4A promoter because the specific 140 bp product (black arrow, 140) is clearly visible whether standard Taq polymerase (no hot start) or AmpliTaq Gold (hot start) is used. (B) dGTP gives rise to unspecific byproducts (black arrow, by), which are reduced if hot start PCR is performed. The 600 bp band of the 100 bp ladder standard is indicated (white arrow, 600).

    Article Snippet: The PCR mixtures contained 1× PCR buffer (Taq polymerase: 50mM KCl, 100mM Tris/HCl, pH 9.0, 0.1% (vol/vol) Triton-X 100, or AmpliTaq Gold polymerase: PCR Gold buffer (Applied Biosystems, Weiterstadt, Germany)), 1.5mM (p16INK4A ) or 2.0mM (HUMARA) magnesium chloride, 200μM dNTP mix (both) or deaza-mix (deaza-dGTP instead of dGTP), 10 pmol of each primer (p16INK4A WT , HUMARA ), 1 U Taq polymerase (Promega, Heidelberg, Germany) or for hot start PCR AmpliTaq Gold (Applied Biosystems) and 50 ng template (p16INK4A , SW1116; HUMARA, ARH77) in a final volume of 25 μl.

    Techniques: Polymerase Chain Reaction, Hot Start PCR

    Nested-PCR assay. (A) MluI qPCR assay to detect pXMRV1 contamination. The 5′ end of the probe spans the MluI restriction site that was introduced to create pXMRV1. pAO-H4, which does not have the MluI restriction site, has lower peak fluorescence as well as a delay in C T s for the same copy numbers of plasmid. (B) Sensitivity of the IAP qPCR assay for different amounts of C57BL/6 mouse DNA ranging from 62.5 ng to 625 ag, all in the presence of 400 ng of human placental DNA. (C) Nested-PCR assay of a small set of samples, showing ∼5% of the samples to be positive for MLV gag ). LNCaP genomic DNA is shown in lanes G. The lane labeled “−” represents the negative control. Lane l shows the 100-bp ladder. (D) Detection of mouse DNA in Platinum Taq (IP Taq, Invitrogen) and Recombinant Taq (IR Taq, Invitrogen) but not in AmpliTaq Gold (AAG Taq, Applied Biosystems). For each qPCR assay, the left column shows the number of replicates that are positive, and the right column shows the average C T at which positivity occurred. The more-XMRV-specific pol qPCR assay (in triplicate) was consistently negative, but IAP and gag assays (eight replicates each) were both positive, as more Platinum or Recombinant Invitrogen Taq was used as a template.

    Journal: Journal of Virology

    Article Title: Absence of XMRV Retrovirus and Other Murine Leukemia Virus-Related Viruses in Patients with Chronic Fatigue Syndrome ▿Absence of XMRV Retrovirus and Other Murine Leukemia Virus-Related Viruses in Patients with Chronic Fatigue Syndrome ▿ ¶

    doi: 10.1128/JVI.00693-11

    Figure Lengend Snippet: Nested-PCR assay. (A) MluI qPCR assay to detect pXMRV1 contamination. The 5′ end of the probe spans the MluI restriction site that was introduced to create pXMRV1. pAO-H4, which does not have the MluI restriction site, has lower peak fluorescence as well as a delay in C T s for the same copy numbers of plasmid. (B) Sensitivity of the IAP qPCR assay for different amounts of C57BL/6 mouse DNA ranging from 62.5 ng to 625 ag, all in the presence of 400 ng of human placental DNA. (C) Nested-PCR assay of a small set of samples, showing ∼5% of the samples to be positive for MLV gag ). LNCaP genomic DNA is shown in lanes G. The lane labeled “−” represents the negative control. Lane l shows the 100-bp ladder. (D) Detection of mouse DNA in Platinum Taq (IP Taq, Invitrogen) and Recombinant Taq (IR Taq, Invitrogen) but not in AmpliTaq Gold (AAG Taq, Applied Biosystems). For each qPCR assay, the left column shows the number of replicates that are positive, and the right column shows the average C T at which positivity occurred. The more-XMRV-specific pol qPCR assay (in triplicate) was consistently negative, but IAP and gag assays (eight replicates each) were both positive, as more Platinum or Recombinant Invitrogen Taq was used as a template.

    Article Snippet: Applied Biosystems' AmpliTaq Gold Taq polymerase contained in the master mix of all of our qPCR TaqMan assays did not contain any IAP sequences ( D), indicating that it was free of mouse DNA.

    Techniques: Nested PCR, Real-time Polymerase Chain Reaction, Fluorescence, Plasmid Preparation, Labeling, Negative Control, Recombinant