amplitaq gold dna polymerase (Thermo Fisher)


Name:
AmpliTaq Gold DNA Polymerase
Description:
AmpliTaq Gold DNA Polymerase is a chemically modified form of AmpliTaq DNA Polymerase requiring thermal activation Features of this enzyme • Automated chemical hot start enzyme for increased specificity sensitivity and yield• Time released thermal activation improves sensitivity in low copy number amplifications• Successful multiplex PCR saves time and reagents• Includes GeneAmp 10X PCR Gold Buffer with MgCl2Hot start activationThe modified enzyme is provided in an inactive state Upon thermal activation the modifier is permanently released regenerating active enzyme The resulting hot start PCR amplification provides increased sensitivity specificity and yield over conventional PCR techniques Higher specificity higher yieldAmpliTaq Gold DNA Polymerase can be activated partially or completely in a pre PCR heat step or can be allowed to activate slowly in a time released manner during the denaturation steps of thermal cycling With or without a limited up front heat activation step active enzyme is released slowly during thermal cycling to match template concentration and increase specificity The yield of specific product increases because reactants are not wasted in the formation of unintended products Because AmpliTaq Gold DNA Polymerase is a chemical hot start enzyme there is no worry of biological contamination GeneAmp 10X PCR Gold Buffer is formulated to provide flexible efficient activation of AmpliTaq Gold DNA Polymerase resulting in a highly specific robust PCR amplification The ionic strength and the pH of GeneAmp 10X PCR Gold Buffer have been optimized to provide a wider activation temperature and time range when used in conjunction with AmpliTaq Gold DNA Polymerase AmpliTaq Gold 360 DNA Polymerase for superior performanceAlso available is AmpliTaq Gold 360 DNA Polymerase for even better PCR performance Compared to AmpliTaq Gold DNA Polymerase AmpliTaq Gold 360 DNA Polymerase is purified by an additional proprietary separation process to eliminate contaminating bacterial DNA sequences from the enzyme preparation When used with the enhanced 360 Gold Buffer this ultra pure enzyme in addition to its hot start capabilities reduces false positive results amplifies low level target sequences and promotes the amplification of a variety of templates Also included with this product is a 360 GC Enhancer to work through challenging GC rich templates
Catalog Number:
4311806
Price:
None
Category:
Proteins Enzymes Peptides
Applications:
Hot Start PCR|PCR|PCR & Real-Time PCR|PCR Enzymes & Master Mixes
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Structured Review
AmpliTaq Gold DNA Polymerase is a chemically modified form of AmpliTaq DNA Polymerase requiring thermal activation Features of this enzyme • Automated chemical hot start enzyme for increased specificity sensitivity and yield• Time released thermal activation improves sensitivity in low copy number amplifications• Successful multiplex PCR saves time and reagents• Includes GeneAmp 10X PCR Gold Buffer with MgCl2Hot start activationThe modified enzyme is provided in an inactive state Upon thermal activation the modifier is permanently released regenerating active enzyme The resulting hot start PCR amplification provides increased sensitivity specificity and yield over conventional PCR techniques Higher specificity higher yieldAmpliTaq Gold DNA Polymerase can be activated partially or completely in a pre PCR heat step or can be allowed to activate slowly in a time released manner during the denaturation steps of thermal cycling With or without a limited up front heat activation step active enzyme is released slowly during thermal cycling to match template concentration and increase specificity The yield of specific product increases because reactants are not wasted in the formation of unintended products Because AmpliTaq Gold DNA Polymerase is a chemical hot start enzyme there is no worry of biological contamination GeneAmp 10X PCR Gold Buffer is formulated to provide flexible efficient activation of AmpliTaq Gold DNA Polymerase resulting in a highly specific robust PCR amplification The ionic strength and the pH of GeneAmp 10X PCR Gold Buffer have been optimized to provide a wider activation temperature and time range when used in conjunction with AmpliTaq Gold DNA Polymerase AmpliTaq Gold 360 DNA Polymerase for superior performanceAlso available is AmpliTaq Gold 360 DNA Polymerase for even better PCR performance Compared to AmpliTaq Gold DNA Polymerase AmpliTaq Gold 360 DNA Polymerase is purified by an additional proprietary separation process to eliminate contaminating bacterial DNA sequences from the enzyme preparation When used with the enhanced 360 Gold Buffer this ultra pure enzyme in addition to its hot start capabilities reduces false positive results amplifies low level target sequences and promotes the amplification of a variety of templates Also included with this product is a 360 GC Enhancer to work through challenging GC rich templates
https://www.bioz.com/result/amplitaq gold dna polymerase/product/Thermo Fisher
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Price from $9.99 to $1999.99
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Gel Extraction:Article Title: Variants of a Thermus aquaticus DNA Polymerase with Increased Selectivity for Applications in Allele- and Methylation-Specific Amplification Article Snippet: .. Reagents and Instruments Oligonucleotides were purchased from Biomers or Metabion, HeLa genomic DNA and Taq 2x master mix was bought from New England Biolabs, dNTPs were either from Roche or Fermentas, Phusion DNA polymerase was purchased from Thermo Scientific, Platinum Taq and Polymerase Chain Reaction:Article Title: 7-Deaza-2`-deoxyguanosine allows PCR and sequencing reactions from CpG islands Article Snippet: We amplified the human p16INK4A promoter (accession number ) and HUMARA exon 1 (accession number ) according to previously published methods. .. The PCR mixtures contained 1× PCR buffer (Taq polymerase: 50mM KCl, 100mM Tris/HCl, pH 9.0, 0.1% (vol/vol) Triton-X 100, or Article Title: Selective control of primer usage in multiplex one-step reverse transcription PCR Article Snippet: One-step RT-PCR (Endpoint) A one-step RT-PCR protocol was used in which the components were combined in a single tube. .. Reaction conditions included 1× PCR buffer (20 mM Tris (pH 8.4), 50 mM KCl) (Invitrogen), 1.5 mM MgCl2 (Invitrogen), gene-specific PCR primers (0.5 μM) (TriLink), oligo(dT)18 primer (1 μM) (TriLink), 0.16 mM dNTPs (New England Biolabs), 0.5 μL of human trachea total RNA or other human total RNA (Stratagene or Ambion, each at ~1.6 μg/μL), 5 U RNase Inhibitor (New England Biolabs), 50 U Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) (Invitrogen) or SuperScript® III Reverse Transcriptase (SSIII RT) (Invitrogen), and 0.6 U of Taq DNA Polymerase, recombinant (Invitrogen) or Platinum® Taq DNA Polymerase (Invitrogen) or Article Title: Tagsteady: a metabarcoding library preparation protocol to avoid false assignment of sequences to samples Article Snippet: Prior to metabarcoding PCRs, for all three primer sets quantitative PCR was carried out to optimise metabarcoding PCR conditions and assess negative extraction controls as described in . .. The 16sIns PCRs were carried out in 25 μl reactions containing 1 μl Hot Start PCR:Article Title: 7-Deaza-2`-deoxyguanosine allows PCR and sequencing reactions from CpG islands Article Snippet: We amplified the human p16INK4A promoter (accession number ) and HUMARA exon 1 (accession number ) according to previously published methods. .. The PCR mixtures contained 1× PCR buffer (Taq polymerase: 50mM KCl, 100mM Tris/HCl, pH 9.0, 0.1% (vol/vol) Triton-X 100, or other:Article Title: A novel MALDI-TOF based methodology for genotyping single nucleotide polymorphisms Article Snippet: Recombinant:Article Title: Selective control of primer usage in multiplex one-step reverse transcription PCR Article Snippet: One-step RT-PCR (Endpoint) A one-step RT-PCR protocol was used in which the components were combined in a single tube. .. Reaction conditions included 1× PCR buffer (20 mM Tris (pH 8.4), 50 mM KCl) (Invitrogen), 1.5 mM MgCl2 (Invitrogen), gene-specific PCR primers (0.5 μM) (TriLink), oligo(dT)18 primer (1 μM) (TriLink), 0.16 mM dNTPs (New England Biolabs), 0.5 μL of human trachea total RNA or other human total RNA (Stratagene or Ambion, each at ~1.6 μg/μL), 5 U RNase Inhibitor (New England Biolabs), 50 U Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) (Invitrogen) or SuperScript® III Reverse Transcriptase (SSIII RT) (Invitrogen), and 0.6 U of Taq DNA Polymerase, recombinant (Invitrogen) or Platinum® Taq DNA Polymerase (Invitrogen) or Real-time Polymerase Chain Reaction:Article Title: Absence of XMRV Retrovirus and Other Murine Leukemia Virus-Related Viruses in Patients with Chronic Fatigue Syndrome ▿Absence of XMRV Retrovirus and Other Murine Leukemia Virus-Related Viruses in Patients with Chronic Fatigue Syndrome ▿ ¶ Article Snippet: .. Applied Biosystems' Ancient DNA Assay:Article Title: Patterns of nucleotide misincorporations during enzymatic amplification and direct large-scale sequencing of ancient DNA Article Snippet: .. These oligonucleotides were used as templates in PCRs with two DNA polymerase reagents, |