amplitaq gold dna polymerase  (Thermo Fisher)


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    Name:
    AmpliTaq Gold DNA Polymerase
    Description:
    AmpliTaq Gold DNA Polymerase is a chemically modified form of AmpliTaq DNA Polymerase requiring thermal activation Features of this enzyme • Automated chemical hot start enzyme for increased specificity sensitivity and yield• Time released thermal activation improves sensitivity in low copy number amplifications• Successful multiplex PCR saves time and reagents• Includes GeneAmp 10X PCR Gold Buffer with MgCl2Hot start activationThe modified enzyme is provided in an inactive state Upon thermal activation the modifier is permanently released regenerating active enzyme The resulting hot start PCR amplification provides increased sensitivity specificity and yield over conventional PCR techniques Higher specificity higher yieldAmpliTaq Gold DNA Polymerase can be activated partially or completely in a pre PCR heat step or can be allowed to activate slowly in a time released manner during the denaturation steps of thermal cycling With or without a limited up front heat activation step active enzyme is released slowly during thermal cycling to match template concentration and increase specificity The yield of specific product increases because reactants are not wasted in the formation of unintended products Because AmpliTaq Gold DNA Polymerase is a chemical hot start enzyme there is no worry of biological contamination GeneAmp 10X PCR Gold Buffer is formulated to provide flexible efficient activation of AmpliTaq Gold DNA Polymerase resulting in a highly specific robust PCR amplification The ionic strength and the pH of GeneAmp 10X PCR Gold Buffer have been optimized to provide a wider activation temperature and time range when used in conjunction with AmpliTaq Gold DNA Polymerase AmpliTaq Gold 360 DNA Polymerase for superior performanceAlso available is AmpliTaq Gold 360 DNA Polymerase for even better PCR performance Compared to AmpliTaq Gold DNA Polymerase AmpliTaq Gold 360 DNA Polymerase is purified by an additional proprietary separation process to eliminate contaminating bacterial DNA sequences from the enzyme preparation When used with the enhanced 360 Gold Buffer this ultra pure enzyme in addition to its hot start capabilities reduces false positive results amplifies low level target sequences and promotes the amplification of a variety of templates Also included with this product is a 360 GC Enhancer to work through challenging GC rich templates
    Catalog Number:
    4311806
    Price:
    None
    Category:
    Proteins Enzymes Peptides
    Applications:
    Hot Start PCR|PCR|PCR & Real-Time PCR|PCR Enzymes & Master Mixes
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    Structured Review

    Thermo Fisher amplitaq gold dna polymerase
    AmpliTaq Gold DNA Polymerase is a chemically modified form of AmpliTaq DNA Polymerase requiring thermal activation Features of this enzyme • Automated chemical hot start enzyme for increased specificity sensitivity and yield• Time released thermal activation improves sensitivity in low copy number amplifications• Successful multiplex PCR saves time and reagents• Includes GeneAmp 10X PCR Gold Buffer with MgCl2Hot start activationThe modified enzyme is provided in an inactive state Upon thermal activation the modifier is permanently released regenerating active enzyme The resulting hot start PCR amplification provides increased sensitivity specificity and yield over conventional PCR techniques Higher specificity higher yieldAmpliTaq Gold DNA Polymerase can be activated partially or completely in a pre PCR heat step or can be allowed to activate slowly in a time released manner during the denaturation steps of thermal cycling With or without a limited up front heat activation step active enzyme is released slowly during thermal cycling to match template concentration and increase specificity The yield of specific product increases because reactants are not wasted in the formation of unintended products Because AmpliTaq Gold DNA Polymerase is a chemical hot start enzyme there is no worry of biological contamination GeneAmp 10X PCR Gold Buffer is formulated to provide flexible efficient activation of AmpliTaq Gold DNA Polymerase resulting in a highly specific robust PCR amplification The ionic strength and the pH of GeneAmp 10X PCR Gold Buffer have been optimized to provide a wider activation temperature and time range when used in conjunction with AmpliTaq Gold DNA Polymerase AmpliTaq Gold 360 DNA Polymerase for superior performanceAlso available is AmpliTaq Gold 360 DNA Polymerase for even better PCR performance Compared to AmpliTaq Gold DNA Polymerase AmpliTaq Gold 360 DNA Polymerase is purified by an additional proprietary separation process to eliminate contaminating bacterial DNA sequences from the enzyme preparation When used with the enhanced 360 Gold Buffer this ultra pure enzyme in addition to its hot start capabilities reduces false positive results amplifies low level target sequences and promotes the amplification of a variety of templates Also included with this product is a 360 GC Enhancer to work through challenging GC rich templates
    https://www.bioz.com/result/amplitaq gold dna polymerase/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    amplitaq gold dna polymerase - by Bioz Stars, 2021-05
    99/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: Tagsteady: a metabarcoding library preparation protocol to avoid false assignment of sequences to samples
    Article Snippet: Prior to metabarcoding PCRs, for all three primer sets quantitative PCR was carried out to optimise metabarcoding PCR conditions and assess negative extraction controls as described in . .. The 16sIns PCRs were carried out in 25 μl reactions containing 1 μl template DNA, 1 U AmpliTaq Gold, 1x Gold PCR Buffer and 2.5 mM MgCl2 (all from Applied Biosystems), 0.2 mM dNTP mix (Invitrogen), 0.5 mg/ml BSA, 0.6 μM of each 5’ nucleotide-tagged forward and reverse primer. .. PCR conditions for 16sIns were as follows: 95°C for 10 minutes, 35 cycles of 95°C for 15 seconds, 54°C for 30 seconds and 72°C for 30 seconds and a seven minutes final extension at 72°C.

    Article Title: Confronting two biomolecular techniques to detect NRF2 gene polymorphism biomarkers
    Article Snippet: Since F2 (rs6721961) and R1 (rs35652124) have melting temperature significantly different from the others, CTPP–PCR reactions were performed using the gradient option of the thermocycler (Multigene optimax thermal cycler, Aurogene SRL, Italy) that enables contemporary polymerization reaction at two different annealing temperatures (Ta). .. All PCRs were performed with 1 unit/sample of Kapa Hot Start Taq polymerase, Biosystems Cat. KK1508 (Sigma-Aldrich, MO, USA) except for the PCR shown in where 2.5 units/sample of AmpliTaq Gold polymerase, Applied Biosystems Cat. N8080161 (ThermoFisher Scientific, MA, USA) were used. .. PCR products were separated on 1.5% agarose gel Cat. BMR 918100 (Euroclone, MI, Italy) with TBE (Tris, Boric acid, EDTA) buffer and stained with gel red staining solution (Biotium, CA, USA).

    Article Title: Selective control of primer usage in multiplex one-step reverse transcription PCR
    Article Snippet: One-step RT-PCR (Endpoint) A one-step RT-PCR protocol was used in which the components were combined in a single tube. .. Reaction conditions included 1× PCR buffer (20 mM Tris (pH 8.4), 50 mM KCl) (Invitrogen), 1.5 mM MgCl2 (Invitrogen), gene-specific PCR primers (0.5 μM) (TriLink), oligo(dT)18 primer (1 μM) (TriLink), 0.16 mM dNTPs (New England Biolabs), 0.5 μL of human trachea total RNA or other human total RNA (Stratagene or Ambion, each at ~1.6 μg/μL), 5 U RNase Inhibitor (New England Biolabs), 50 U Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) (Invitrogen) or SuperScript® III Reverse Transcriptase (SSIII RT) (Invitrogen), and 0.6 U of Taq DNA Polymerase, recombinant (Invitrogen) or Platinum® Taq DNA Polymerase (Invitrogen) or AmpliTaq Gold® DNA Polymerase (Applied Biosystems), in a 50 μL reaction volume. .. Thermal cycling conditions utilized a reverse transcription step at 42°C for 30 min when M-MLV RT was used and 55°C for 30 min when SSIII RT was employed; 95°C for 10 min (RT inactivation and initial denaturation step), followed by 45 PCR cycles at 95°C for 30 sec, 60°C for 1 min and final extension at 72°C for 5 min.

    Article Title: 7-Deaza-2`-deoxyguanosine allows PCR and sequencing reactions from CpG islands
    Article Snippet: We amplified the human p16INK4A promoter (accession number ) and HUMARA exon 1 (accession number ) according to previously published methods. .. The PCR mixtures contained 1× PCR buffer (Taq polymerase: 50mM KCl, 100mM Tris/HCl, pH 9.0, 0.1% (vol/vol) Triton-X 100, or AmpliTaq Gold polymerase: PCR Gold buffer (Applied Biosystems, Weiterstadt, Germany)), 1.5mM (p16INK4A ) or 2.0mM (HUMARA) magnesium chloride, 200μM dNTP mix (both) or deaza-mix (deaza-dGTP instead of dGTP), 10 pmol of each primer (p16INK4A WT , HUMARA ), 1 U Taq polymerase (Promega, Heidelberg, Germany) or for hot start PCR AmpliTaq Gold (Applied Biosystems) and 50 ng template (p16INK4A , SW1116; HUMARA, ARH77) in a final volume of 25 μl. .. The following PCR cycle profiles were used. p16INK4A : five minutes (Taq polymerase) or 12 minutes (AmpliTaq Gold) at 94°C; 35 cycles of 20 seconds at 94°C, 20 seconds at 65°C, and 20 seconds at 72°C; followed by two minutes at 72°C.

    Recombinant:

    Article Title: Selective control of primer usage in multiplex one-step reverse transcription PCR
    Article Snippet: One-step RT-PCR (Endpoint) A one-step RT-PCR protocol was used in which the components were combined in a single tube. .. Reaction conditions included 1× PCR buffer (20 mM Tris (pH 8.4), 50 mM KCl) (Invitrogen), 1.5 mM MgCl2 (Invitrogen), gene-specific PCR primers (0.5 μM) (TriLink), oligo(dT)18 primer (1 μM) (TriLink), 0.16 mM dNTPs (New England Biolabs), 0.5 μL of human trachea total RNA or other human total RNA (Stratagene or Ambion, each at ~1.6 μg/μL), 5 U RNase Inhibitor (New England Biolabs), 50 U Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) (Invitrogen) or SuperScript® III Reverse Transcriptase (SSIII RT) (Invitrogen), and 0.6 U of Taq DNA Polymerase, recombinant (Invitrogen) or Platinum® Taq DNA Polymerase (Invitrogen) or AmpliTaq Gold® DNA Polymerase (Applied Biosystems), in a 50 μL reaction volume. .. Thermal cycling conditions utilized a reverse transcription step at 42°C for 30 min when M-MLV RT was used and 55°C for 30 min when SSIII RT was employed; 95°C for 10 min (RT inactivation and initial denaturation step), followed by 45 PCR cycles at 95°C for 30 sec, 60°C for 1 min and final extension at 72°C for 5 min.

    other:

    Article Title: A novel MALDI-TOF based methodology for genotyping single nucleotide polymorphisms
    Article Snippet: AmpliTaq Gold® DNA polymerase was purchased from Applied Biosystems (Foster City, CA).

    Ancient DNA Assay:

    Article Title: Patterns of nucleotide misincorporations during enzymatic amplification and direct large-scale sequencing of ancient DNA
    Article Snippet: .. These oligonucleotides were used as templates in PCRs with two DNA polymerase reagents, AmpliTaq Gold (Applied Biosystems, Foster City, CA) and Platinum Taq High Fidelity (Invitrogen, Carlsbad, CA), which are both commonly used in ancient DNA research. .. In contrast, when the oligonucleotide carrying the U was used, both polymerase reagents inserted A residues opposite the template sites carrying a U residue in all clones sequenced (data not shown).

    Real-time Polymerase Chain Reaction:

    Article Title: Absence of XMRV Retrovirus and Other Murine Leukemia Virus-Related Viruses in Patients with Chronic Fatigue Syndrome ▿Absence of XMRV Retrovirus and Other Murine Leukemia Virus-Related Viruses in Patients with Chronic Fatigue Syndrome ▿ ¶
    Article Snippet: .. Applied Biosystems' AmpliTaq Gold Taq polymerase contained in the master mix of all of our qPCR TaqMan assays did not contain any IAP sequences ( D), indicating that it was free of mouse DNA. .. When additional AmpliTaq Gold Taq polymerase was added as a template for the IAP qPCR assay, as was done with the other polymerase preparations, all reactions remained negative.

    Hot Start PCR:

    Article Title: 7-Deaza-2`-deoxyguanosine allows PCR and sequencing reactions from CpG islands
    Article Snippet: We amplified the human p16INK4A promoter (accession number ) and HUMARA exon 1 (accession number ) according to previously published methods. .. The PCR mixtures contained 1× PCR buffer (Taq polymerase: 50mM KCl, 100mM Tris/HCl, pH 9.0, 0.1% (vol/vol) Triton-X 100, or AmpliTaq Gold polymerase: PCR Gold buffer (Applied Biosystems, Weiterstadt, Germany)), 1.5mM (p16INK4A ) or 2.0mM (HUMARA) magnesium chloride, 200μM dNTP mix (both) or deaza-mix (deaza-dGTP instead of dGTP), 10 pmol of each primer (p16INK4A WT , HUMARA ), 1 U Taq polymerase (Promega, Heidelberg, Germany) or for hot start PCR AmpliTaq Gold (Applied Biosystems) and 50 ng template (p16INK4A , SW1116; HUMARA, ARH77) in a final volume of 25 μl. .. The following PCR cycle profiles were used. p16INK4A : five minutes (Taq polymerase) or 12 minutes (AmpliTaq Gold) at 94°C; 35 cycles of 20 seconds at 94°C, 20 seconds at 65°C, and 20 seconds at 72°C; followed by two minutes at 72°C.

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  • 99
    Thermo Fisher amplitaq gold dna polymerase
    Marker TSC0033440 (G/C polymorphism). Exo I- and SAP-treated PCR products, genotyped without ddNTPs, re-using <t>AmpliTaq</t> Gold, from the PCR step on three pre-genotyped <t>DNA</t> samples. Reverse informative primer (5622 Da) was used for the primer extension with 25 µM dNTP depleted of dGTP. The expected extension products were 5936 Da (pA extension) and 6538 Da (pApCpA extension) for the C and G alleles, respectively. ( A ) Homozygous C/C sample for TSC0033440. ( B ) Heterozygous G/C sample for TSC0033440. ( C ) Homozygous G/G sample for TSC0033440.
    Amplitaq Gold Dna Polymerase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/amplitaq gold dna polymerase/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    amplitaq gold dna polymerase - by Bioz Stars, 2021-05
    99/100 stars
      Buy from Supplier

    94
    Thermo Fisher amplitaq gold 360 dna polymerase
    Inhibition and the effect of hot-start. ( a ) Inhibition profiles of 2D9 versus <t>AmpliTaq</t> <t>Gold</t> 360 (ATaqG) in PCR. The effect of various inhibitors (SP61: bone dust, Tar, Soil, 1736; cave sediment) on polymerase activity of 2D9 and ATaqG as determined by a PCR assay in the presence of decreasing amounts of inhibitor and plotted as the average relative resistance ( n = 3, standard error) (as in Figure 4 ) of 2D9 and ATaqG with respect to ATaqG as a reference (resistance = 1). ( b ) The effect of manual hot-start on resistance of 2D9 polymerase to inhibitors tar and soil at minimal limiting amounts of inhibitor. Hot-start increases inhibitor resistance by at least a factor of two.
    Amplitaq Gold 360 Dna Polymerase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/amplitaq gold 360 dna polymerase/product/Thermo Fisher
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    amplitaq gold 360 dna polymerase - by Bioz Stars, 2021-05
    94/100 stars
      Buy from Supplier

    Image Search Results


    Marker TSC0033440 (G/C polymorphism). Exo I- and SAP-treated PCR products, genotyped without ddNTPs, re-using AmpliTaq Gold, from the PCR step on three pre-genotyped DNA samples. Reverse informative primer (5622 Da) was used for the primer extension with 25 µM dNTP depleted of dGTP. The expected extension products were 5936 Da (pA extension) and 6538 Da (pApCpA extension) for the C and G alleles, respectively. ( A ) Homozygous C/C sample for TSC0033440. ( B ) Heterozygous G/C sample for TSC0033440. ( C ) Homozygous G/G sample for TSC0033440.

    Journal: Nucleic Acids Research

    Article Title: A novel MALDI-TOF based methodology for genotyping single nucleotide polymorphisms

    doi: 10.1093/nar/gng156

    Figure Lengend Snippet: Marker TSC0033440 (G/C polymorphism). Exo I- and SAP-treated PCR products, genotyped without ddNTPs, re-using AmpliTaq Gold, from the PCR step on three pre-genotyped DNA samples. Reverse informative primer (5622 Da) was used for the primer extension with 25 µM dNTP depleted of dGTP. The expected extension products were 5936 Da (pA extension) and 6538 Da (pApCpA extension) for the C and G alleles, respectively. ( A ) Homozygous C/C sample for TSC0033440. ( B ) Heterozygous G/C sample for TSC0033440. ( C ) Homozygous G/G sample for TSC0033440.

    Article Snippet: AmpliTaq Gold® DNA polymerase was purchased from Applied Biosystems (Foster City, CA).

    Techniques: Marker, Polymerase Chain Reaction

    Confronting two-pair primers–polymerase chain reaction for NRF2 -617C/A and -653A/G was carried out at the indicated T a using 2.5U/sample of AmpliTaq Gold polymerase. N: Negative control indicates DNA template omission. MW: Molecular weights (100 bp up to 1000 bp).

    Journal: Future Science OA

    Article Title: Confronting two biomolecular techniques to detect NRF2 gene polymorphism biomarkers

    doi: 10.4155/fsoa-2018-0075

    Figure Lengend Snippet: Confronting two-pair primers–polymerase chain reaction for NRF2 -617C/A and -653A/G was carried out at the indicated T a using 2.5U/sample of AmpliTaq Gold polymerase. N: Negative control indicates DNA template omission. MW: Molecular weights (100 bp up to 1000 bp).

    Article Snippet: All PCRs were performed with 1 unit/sample of Kapa Hot Start Taq polymerase, Biosystems Cat. KK1508 (Sigma-Aldrich, MO, USA) except for the PCR shown in where 2.5 units/sample of AmpliTaq Gold polymerase, Applied Biosystems Cat. N8080161 (ThermoFisher Scientific, MA, USA) were used.

    Techniques: Polymerase Chain Reaction, Negative Control

    Summary of the oligonucleotide competition amplifications. Each bar represents the ratio of nucleotides found among clones from amplification products generated from a mixture of two oligonucleotides, given by the abbreviations below the bars. Misincorporated nucleotides are indicated by the black upper part of each bar, and numbers give the numbers of clones sequenced. The polymerase reagents are indicated as “Gold” for AmpliTaq Gold and “Platin” for Platinum Taq High Fidelity.

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Patterns of nucleotide misincorporations during enzymatic amplification and direct large-scale sequencing of ancient DNA

    doi: 10.1073/pnas.0605327103

    Figure Lengend Snippet: Summary of the oligonucleotide competition amplifications. Each bar represents the ratio of nucleotides found among clones from amplification products generated from a mixture of two oligonucleotides, given by the abbreviations below the bars. Misincorporated nucleotides are indicated by the black upper part of each bar, and numbers give the numbers of clones sequenced. The polymerase reagents are indicated as “Gold” for AmpliTaq Gold and “Platin” for Platinum Taq High Fidelity.

    Article Snippet: These oligonucleotides were used as templates in PCRs with two DNA polymerase reagents, AmpliTaq Gold (Applied Biosystems, Foster City, CA) and Platinum Taq High Fidelity (Invitrogen, Carlsbad, CA), which are both commonly used in ancient DNA research.

    Techniques: Clone Assay, Amplification, Generated

    Inhibition and the effect of hot-start. ( a ) Inhibition profiles of 2D9 versus AmpliTaq Gold 360 (ATaqG) in PCR. The effect of various inhibitors (SP61: bone dust, Tar, Soil, 1736; cave sediment) on polymerase activity of 2D9 and ATaqG as determined by a PCR assay in the presence of decreasing amounts of inhibitor and plotted as the average relative resistance ( n = 3, standard error) (as in Figure 4 ) of 2D9 and ATaqG with respect to ATaqG as a reference (resistance = 1). ( b ) The effect of manual hot-start on resistance of 2D9 polymerase to inhibitors tar and soil at minimal limiting amounts of inhibitor. Hot-start increases inhibitor resistance by at least a factor of two.

    Journal: Nucleic Acids Research

    Article Title: Molecular breeding of polymerases for resistance to environmental inhibitors

    doi: 10.1093/nar/gkq1360

    Figure Lengend Snippet: Inhibition and the effect of hot-start. ( a ) Inhibition profiles of 2D9 versus AmpliTaq Gold 360 (ATaqG) in PCR. The effect of various inhibitors (SP61: bone dust, Tar, Soil, 1736; cave sediment) on polymerase activity of 2D9 and ATaqG as determined by a PCR assay in the presence of decreasing amounts of inhibitor and plotted as the average relative resistance ( n = 3, standard error) (as in Figure 4 ) of 2D9 and ATaqG with respect to ATaqG as a reference (resistance = 1). ( b ) The effect of manual hot-start on resistance of 2D9 polymerase to inhibitors tar and soil at minimal limiting amounts of inhibitor. Hot-start increases inhibitor resistance by at least a factor of two.

    Article Snippet: Inhibition profiles for AmpliTaq Gold 360 DNA polymerase (Applied Biosystems) were determined using an identical PCR set-up but using AmpliTaq Gold buffer and thermocycling conditions according to manufacturer’s recommendations.

    Techniques: Inhibition, Polymerase Chain Reaction, Activity Assay