amplitaq gold dna polymerase (Thermo Fisher)
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amplitaq gold dna polymerase
Amplitaq Gold Dna Polymerase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1609 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/amplitaq gold dna polymerase/product/Thermo Fisher
Average 99 stars, based on 1609 article reviews
Price from $9.99 to $1999.99
Amplitaq Gold Dna Polymerase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1609 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/amplitaq gold dna polymerase/product/Thermo Fisher
Average 99 stars, based on 1609 article reviews
Price from $9.99 to $1999.99
amplitaq gold dna polymerase - by Bioz Stars,
2020-02
99/100 stars
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Clone Assay:Article Title: Priority Report: Rearrangements and Amplification of IER3 (IEX-1) Represent A Novel And Recurrent Molecular Abnormality In Myelodysplastic Syndromes (MDS) Article Snippet: To assess for point mutations in the propositus and other patients with MDS with and without 6p rearrangements, genomic DNA purified with QIAamp DNA Blood Mini Kit (QIAgen, Venlo, The Netherlands) was amplified by PCR on a PTC-200 Peltier thermocycler (MJ Research, Waltham, MA) and sequenced as for breakpoint cloning. .. Reagents included GeneAmp® PCR Buffer II (Applied Biosystems), 1.5 mM MgCl2 (Applied Biosystems), 200 μM dNTPs (Roche, Mannheim, Germany), forward and reverse oligonucleotide primers (see ), 100 ng of template DNA, and Centrifugation:Article Title: Multilocus Variable-Number Tandem-Repeat Analysis for Investigation of Clostridium difficile Transmission in Hospitals Article Snippet: PCRs were performed in 50-μl volumes containing 1× AmpliTaq Gold PCR buffer (15 mM Tris-HCl, pH 8.0, 50 mM KCl), 2.5 mM MgCl2 , 0.2 mM each deoxynucleoside triphosphate, 0.2 μM of each primer, and 1.5 units Amplification:Article Title: DNA Repair Gene Polymorphisms May Be Associated with Prognosis of Upper Urinary Tract Transitional Cell Carcinoma 1 Article Snippet: .. Briefly, the DNA fragments were amplified from 10 ng of DNA in 10 µl of PCR reactions containing 1.5 mM MgCl2 , 0.2 mM each deoxyribonucleotide triphosphate, 0.3 U of Article Title: Parathyroid tumor development involves deregulation of homeobox genes Article Snippet: .. Genomic DNA was amplified by PCR using the following conditions: 10 ng genomic DNA, 0.2 mM each primer, 200 mM each dNTP, 2 mM MgCl2 , 0.5 units Article Title: Priority Report: Rearrangements and Amplification of IER3 (IEX-1) Represent A Novel And Recurrent Molecular Abnormality In Myelodysplastic Syndromes (MDS) Article Snippet: To assess for point mutations in the propositus and other patients with MDS with and without 6p rearrangements, genomic DNA purified with QIAamp DNA Blood Mini Kit (QIAgen, Venlo, The Netherlands) was amplified by PCR on a PTC-200 Peltier thermocycler (MJ Research, Waltham, MA) and sequenced as for breakpoint cloning. .. Reagents included GeneAmp® PCR Buffer II (Applied Biosystems), 1.5 mM MgCl2 (Applied Biosystems), 200 μM dNTPs (Roche, Mannheim, Germany), forward and reverse oligonucleotide primers (see ), 100 ng of template DNA, and Article Title: The influence of FKBP5 genotype on expression of FKBP5 and other glucocorticoid-regulated genes, dependent on trauma exposure Article Snippet: .. PCR amplification with primers ( ) for FKBP5 was performed using Article Title: Influence of Antibiotic Selection on Genetic Composition of Escherichia coli Populations from Conventional and Organic Dairy Farms ▿ Populations from Conventional and Organic Dairy Farms ▿ † Article Snippet: Genomic DNA preparations were tested using primers targeting a 650-bp region of the conserved housekeeping gene mdh (see for primer sequences and reaction conditions), and Article Title: Oral microbiome diversity in chimpanzees from Gombe National Park Article Snippet: .. Following shotgun preparation, samples were amplified using Article Title: Hyperglycosylation and Reduced GABA Currents of Mutated GABRB3 Polypeptide in Remitting Childhood Absence Epilepsy Article Snippet: We also amplified all coding regions from exon 4 to exon 9, including splice sites, and the selected regions from intron 3 and 3′ UTR. .. For PCR reactions, the Article Title: Mutations of RAS genes in endometrial polyps Article Snippet: .. The NRAS exon 2 was amplified from the extracted DNA by Applied Biosystems Article Title: Establishment of Murine Cytomegalovirus Latency In Vivo Is Associated with Changes in Histone Modifications and Recruitment of Transcriptional Repressors to the Major Immediate-Early Promoter ▿ Article Snippet: .. Semiquantitative PCR amplification of the MCMV MIEP segment was performed in the presence of 400 nM of each primer (Table ), 0.2 mM each of dATP, dCTP, dGTP, and dTTP, 1× PCR buffer, 1.5 mM MgCl2 , 1 unit of Article Title: Tumor suppressive BTB/POZ zinc-finger protein ZBTB28 inhibits oncogenic BCL6/ZBTB27 signaling to maintain p53 transcription in multiple carcinogenesis Article Snippet: MSP analysis revealed no amplified products in any non-bisulfited DNA by MSP primers, thus it can be considered specific. .. Article Title: TZAP Mutation Leads to Poor Prognosis of Patients with Breast Cancer † Article Snippet: Telomeric Zinc Finger-Associated Protein (TZAP) Mutation Analysis The TZAP mutation was amplified from isolated DNA using the polymerase chain reaction (PCR). .. PCR was performed using Article Title: Novel TNXB Variants in Two Italian Patients with Classical-Like Ehlers-Danlos Syndrome Article Snippet: Analysis of the TNXA/TNXB Homology Region For the analysis of the TNXA/TNXB homology region, the whole genomic sequence of TNXB, encompassing exons 32–44 was amplified by employing a Long-PCR reaction using the primers reported in and the protocol previously reported [ ]. .. Reactions were performed in a 25 uL volume reaction containing 2.5 uL Buffer (10 ×), dNTP (0,25 mM final concentration), primers (10 pmol each), 1.25 U Quantitative RT-PCR:Article Title: The influence of FKBP5 genotype on expression of FKBP5 and other glucocorticoid-regulated genes, dependent on trauma exposure Article Snippet: Methods for extraction of RNA and cDNA synthesis are the same as described above in the ‘Quantitative RT-PCR’ section. .. PCR amplification with primers ( ) for FKBP5 was performed using Concentration Assay:Article Title: Influence of Antibiotic Selection on Genetic Composition of Escherichia coli Populations from Conventional and Organic Dairy Farms ▿ Populations from Conventional and Organic Dairy Farms ▿ † Article Snippet: DNA preparations were quantified with a NanoDrop ND-1000 UV-visible spectrophotometer (NanoDrop Technologies, Wilmington, DE), diluted to a final concentration of 100 ng/μl, and stored at 4°C. .. Genomic DNA preparations were tested using primers targeting a 650-bp region of the conserved housekeeping gene mdh (see for primer sequences and reaction conditions), and Article Title: Novel TNXB Variants in Two Italian Patients with Classical-Like Ehlers-Danlos Syndrome Article Snippet: .. Reactions were performed in a 25 uL volume reaction containing 2.5 uL Buffer (10 ×), dNTP (0,25 mM final concentration), primers (10 pmol each), 1.25 U Real-time Polymerase Chain Reaction:Article Title: Establishment of Murine Cytomegalovirus Latency In Vivo Is Associated with Changes in Histone Modifications and Recruitment of Transcriptional Repressors to the Major Immediate-Early Promoter ▿ Article Snippet: Generally, one-third of the immunoprecipitated DNA was used for semiquantitative and/or real-time PCR amplification. .. Semiquantitative PCR amplification of the MCMV MIEP segment was performed in the presence of 400 nM of each primer (Table ), 0.2 mM each of dATP, dCTP, dGTP, and dTTP, 1× PCR buffer, 1.5 mM MgCl2 , 1 unit of Incubation:Article Title: The influence of FKBP5 genotype on expression of FKBP5 and other glucocorticoid-regulated genes, dependent on trauma exposure Article Snippet: To extract genomic DNA, briefly, cells were lysed in 1 % SDS, 0.05 M EDTA, 50 mM Tris–Cl, pH 8.0, and 100 ng/ml proteinase K, and incubated at 55 °C for 16 h. Genomic DNA was purified by phenol/chloroform extraction. .. PCR amplification with primers ( ) for FKBP5 was performed using Article Title: Oral microbiome diversity in chimpanzees from Gombe National Park Article Snippet: For the final fill in portion of the shotgun build, 20 µL of template DNA was added to 4.0 µL Thermo pol buffer, 0.50 µL dNTP mix (2.5 mM), 2.0 µL Bst polymerase, and 13.50 µL ddH2 O was incubated at 37 °C for 20 minutes followed by 80 °C for 20 minutes. .. Following shotgun preparation, samples were amplified using Article Title: Establishment of Murine Cytomegalovirus Latency In Vivo Is Associated with Changes in Histone Modifications and Recruitment of Transcriptional Repressors to the Major Immediate-Early Promoter ▿ Article Snippet: The cross-links were reversed by incubation overnight at 65°C in the presence of 200 mM NaCl followed by incubation with 0.2 μg/μl protease K at 45°C for 1.5 h. The DNA was extracted with phenol-chloroform and precipitated with a 1/10 volume of 3 M sodium acetate, pH 5.2, and 2.5 volumes of ethanol. .. Semiquantitative PCR amplification of the MCMV MIEP segment was performed in the presence of 400 nM of each primer (Table ), 0.2 mM each of dATP, dCTP, dGTP, and dTTP, 1× PCR buffer, 1.5 mM MgCl2 , 1 unit of Formalin-fixed Paraffin-Embedded:Article Title: Mutations of RAS genes in endometrial polyps Article Snippet: Each sample was placed in a 200-µl microtube and DNA was extracted using a NucleoSpin DNA FFPE XS kit (Takara Bio, Inc.). .. The NRAS exon 2 was amplified from the extracted DNA by Applied Biosystems Expressing:Article Title: Tumor suppressive BTB/POZ zinc-finger protein ZBTB28 inhibits oncogenic BCL6/ZBTB27 signaling to maintain p53 transcription in multiple carcinogenesis Article Snippet: Transformation Assay:Article Title: Mutations of RAS genes in endometrial polyps Article Snippet: The NRAS exon 2 was amplified from the extracted DNA by Applied Biosystems High Performance Liquid Chromatography:Article Title: Hyperglycosylation and Reduced GABA Currents of Mutated GABRB3 Polypeptide in Remitting Childhood Absence Epilepsy Article Snippet: For PCR reactions, the Ligation:Article Title: Oral microbiome diversity in chimpanzees from Gombe National Park Article Snippet: For adapter ligation, 18 µL of template DNA was added to 20 µL Quick Ligase Buffer, 1.0 µL Solexa Mix , and 1.0 µL Quick Ligase and incubated at room temperature for 20 minutes. .. Following shotgun preparation, samples were amplified using Cell Culture:Article Title: Mutations of RAS genes in endometrial polyps Article Snippet: The NRAS exon 2 was amplified from the extracted DNA by Applied Biosystems CpG Methylation Assay:Article Title: Tumor suppressive BTB/POZ zinc-finger protein ZBTB28 inhibits oncogenic BCL6/ZBTB27 signaling to maintain p53 transcription in multiple carcinogenesis Article Snippet: Imaging:Article Title: Tumor suppressive BTB/POZ zinc-finger protein ZBTB28 inhibits oncogenic BCL6/ZBTB27 signaling to maintain p53 transcription in multiple carcinogenesis Article Snippet: Sequencing:Article Title: Parathyroid tumor development involves deregulation of homeobox genes Article Snippet: Paragraph title: Sequence analysis of MEN1 ... Genomic DNA was amplified by PCR using the following conditions: 10 ng genomic DNA, 0.2 mM each primer, 200 mM each dNTP, 2 mM MgCl2 , 0.5 units Article Title: Priority Report: Rearrangements and Amplification of IER3 (IEX-1) Represent A Novel And Recurrent Molecular Abnormality In Myelodysplastic Syndromes (MDS) Article Snippet: Amplicons detected by ethidium-bromide-impregnated agarose gel electrophoresis were then sequenced using fluorescent dye chemistry sequencing to explicitly define the rearrangement (ABI Prism 3730 DNA Analyzer; Applied Biosystems, Foster City, CA), with chromatograms compared to , and ( IER3 ) using Sequencher 4.5 software (Gene Codes, Ann Arbor, MI). .. Reagents included GeneAmp® PCR Buffer II (Applied Biosystems), 1.5 mM MgCl2 (Applied Biosystems), 200 μM dNTPs (Roche, Mannheim, Germany), forward and reverse oligonucleotide primers (see ), 100 ng of template DNA, and Article Title: The influence of FKBP5 genotype on expression of FKBP5 and other glucocorticoid-regulated genes, dependent on trauma exposure Article Snippet: Paragraph title: Quantitative Sanger sequencing to detect allelic imbalance in cDNA ... PCR amplification with primers ( ) for FKBP5 was performed using Article Title: The emergence of a new strain of porcine circovirus-2 in Ontario and Quebec swine and its association with severe porcine circovirus associated disease -- 2004-2006 Article Snippet: Primers for initial diagnosis for all Ontario cases were selected using sequence information for both PCV-1 and PCV-2 genomes obtained from GenBank (Accession no. and ). .. The master mix was prepared in 50 μL final volume using 2.5 U Article Title: Oral microbiome diversity in chimpanzees from Gombe National Park Article Snippet: Paragraph title: Shotgun build, amplification, and sequencing ... Following shotgun preparation, samples were amplified using Article Title: Mutations of RAS genes in endometrial polyps Article Snippet: Paragraph title: Laser-capture microdissection and RAS-targeted sequencing ... The NRAS exon 2 was amplified from the extracted DNA by Applied Biosystems Article Title: Multilocus Variable-Number Tandem-Repeat Analysis for Investigation of Clostridium difficile Transmission in Hospitals Article Snippet: PCR amplification and sequence analysis of the seven selected C. difficile repeat (CDR) loci was performed on the 86 C. difficile isolates from UPMC and HVA with the following primer sets: CDR4F (5′-ATTAATCATATCCTACAGAACACGA-3′) and CDR4R (5′-TAAAACAAATGATATAAACTGAAAAG-3′); CDR5F (5′-AATTTTAAGTTAACGTTTTTCTACAT-3′) and CDR5R (5′-AGCCATTTTTATCAATCCTTTCTAT-3′); CDR9F (5′-TCTGGGATGTAACTAGCGACTTGT-3′) and CDR9R (5′-TCTTAGGGAATTTATTGGAGGAA-3′); CDR48F (5′-AGGAGCTTTATATGGACATTCAGGTAG-3′) and CDR48R (5′-AATCTCTTTCAAACTCTTCAATCTCAAT-3′); CDR49F (5′-AACATATTTAGGCATTTTAGTC-3′) and CDR49R (5′-GAGTATTATTTATCATTTGTGGGTATTA-3′); CDR59F (5′-GTAGAAGGGGCAAATAATGAG-3′) and CDR59R (5′-CCTTCTGGCTTCCTTGTA-ATA-3′); and CDR60F (5′-GGTGCACATGCTGGTCCTG-3′) and CDR60R (5′-AACGCATTAAATTTCAC-TCCTCATAC-3′). .. PCRs were performed in 50-μl volumes containing 1× AmpliTaq Gold PCR buffer (15 mM Tris-HCl, pH 8.0, 50 mM KCl), 2.5 mM MgCl2 , 0.2 mM each deoxynucleoside triphosphate, 0.2 μM of each primer, and 1.5 units Article Title: History-driven population structure and asymmetric gene flow in a recovering large carnivore at the rear-edge of its European range Article Snippet: A total of 60 samples, which were determined to belong to different individuals as per the microsatellite analysis, were used to sequence a 271 bp stretch of the mitochondrial control region. .. Amplifications were performed in 15-µl reactions containing 1x Gold Buffer (Applied Biosystems, Foster City, CA, USA), 2.5 mM MgCl2 , 0.2 mM dNTPs, 0.4 µM forward primers, 0.4 µM reverse primer and 0.027 U/µl Article Title: Novel TNXB Variants in Two Italian Patients with Classical-Like Ehlers-Danlos Syndrome Article Snippet: Analysis of the TNXA/TNXB Homology Region For the analysis of the TNXA/TNXB homology region, the whole genomic sequence of TNXB, encompassing exons 32–44 was amplified by employing a Long-PCR reaction using the primers reported in and the protocol previously reported [ ]. .. Reactions were performed in a 25 uL volume reaction containing 2.5 uL Buffer (10 ×), dNTP (0,25 mM final concentration), primers (10 pmol each), 1.25 U DNA Sequencing:Article Title: Priority Report: Rearrangements and Amplification of IER3 (IEX-1) Represent A Novel And Recurrent Molecular Abnormality In Myelodysplastic Syndromes (MDS) Article Snippet: Paragraph title: DNA Sequencing ... Reagents included GeneAmp® PCR Buffer II (Applied Biosystems), 1.5 mM MgCl2 (Applied Biosystems), 200 μM dNTPs (Roche, Mannheim, Germany), forward and reverse oligonucleotide primers (see ), 100 ng of template DNA, and Article Title: TZAP Mutation Leads to Poor Prognosis of Patients with Breast Cancer † Article Snippet: PCR was performed using DNA Extraction:Article Title: DNA Repair Gene Polymorphisms May Be Associated with Prognosis of Upper Urinary Tract Transitional Cell Carcinoma 1 Article Snippet: Paragraph title: DNA Extraction and Genotyping ... Briefly, the DNA fragments were amplified from 10 ng of DNA in 10 µl of PCR reactions containing 1.5 mM MgCl2 , 0.2 mM each deoxyribonucleotide triphosphate, 0.3 U of Article Title: Parathyroid tumor development involves deregulation of homeobox genes Article Snippet: Briefly, patient's frozen sectioned parathyroid tissues were processed for genomic DNA isolation. .. Genomic DNA was amplified by PCR using the following conditions: 10 ng genomic DNA, 0.2 mM each primer, 200 mM each dNTP, 2 mM MgCl2 , 0.5 units Article Title: Influence of Antibiotic Selection on Genetic Composition of Escherichia coli Populations from Conventional and Organic Dairy Farms ▿ Populations from Conventional and Organic Dairy Farms ▿ † Article Snippet: Genomic DNA was isolated from 2 ml of overnight culture in LB broth (Lennox; Becton, Dickinson, and Company, Sparks, MD) using the Puregene DNA isolation kit (Gentra Systems Inc., Minneapolis, MN.). .. Genomic DNA preparations were tested using primers targeting a 650-bp region of the conserved housekeeping gene mdh (see for primer sequences and reaction conditions), and Article Title: History-driven population structure and asymmetric gene flow in a recovering large carnivore at the rear-edge of its European range Article Snippet: Paragraph title: DNA extraction, microsatellite and mitochondrial analysis ... Amplifications were performed in 15-µl reactions containing 1x Gold Buffer (Applied Biosystems, Foster City, CA, USA), 2.5 mM MgCl2 , 0.2 mM dNTPs, 0.4 µM forward primers, 0.4 µM reverse primer and 0.027 U/µl Methylation:Article Title: Tumor suppressive BTB/POZ zinc-finger protein ZBTB28 inhibits oncogenic BCL6/ZBTB27 signaling to maintain p53 transcription in multiple carcinogenesis Article Snippet: Paragraph title: Bisulfite conversion and methylation-specific PCR (MSP) ... Mutagenesis:Article Title: Parathyroid tumor development involves deregulation of homeobox genes Article Snippet: Genomic DNA was amplified by PCR using the following conditions: 10 ng genomic DNA, 0.2 mM each primer, 200 mM each dNTP, 2 mM MgCl2 , 0.5 units Article Title: Hyperglycosylation and Reduced GABA Currents of Mutated GABRB3 Polypeptide in Remitting Childhood Absence Epilepsy Article Snippet: Paragraph title: Mutation Analysis ... For PCR reactions, the Article Title: Mutations of RAS genes in endometrial polyps Article Snippet: The RAS mutation was analyzed in separate glandular and stromal components. .. The NRAS exon 2 was amplified from the extracted DNA by Applied Biosystems Article Title: TZAP Mutation Leads to Poor Prognosis of Patients with Breast Cancer † Article Snippet: Paragraph title: 2.2. Telomeric Zinc Finger-Associated Protein (TZAP) Mutation Analysis ... PCR was performed using Isolation:Article Title: Influence of Antibiotic Selection on Genetic Composition of Escherichia coli Populations from Conventional and Organic Dairy Farms ▿ Populations from Conventional and Organic Dairy Farms ▿ † Article Snippet: Genomic DNA was isolated from 2 ml of overnight culture in LB broth (Lennox; Becton, Dickinson, and Company, Sparks, MD) using the Puregene DNA isolation kit (Gentra Systems Inc., Minneapolis, MN.). .. Genomic DNA preparations were tested using primers targeting a 650-bp region of the conserved housekeeping gene mdh (see for primer sequences and reaction conditions), and Article Title: TZAP Mutation Leads to Poor Prognosis of Patients with Breast Cancer † Article Snippet: Telomeric Zinc Finger-Associated Protein (TZAP) Mutation Analysis The TZAP mutation was amplified from isolated DNA using the polymerase chain reaction (PCR). .. PCR was performed using Article Title: Multilocus Variable-Number Tandem-Repeat Analysis for Investigation of Clostridium difficile Transmission in Hospitals Article Snippet: Genomic C. difficile DNA was isolated from 10-ml tryptic soy broth cultures grown for 18 h anaerobically using the QIAGEN DNeasy MiniKit according to the manufacturer's instructions for gram-positive organisms (QIAGEN, Valencia, CA). .. PCRs were performed in 50-μl volumes containing 1× AmpliTaq Gold PCR buffer (15 mM Tris-HCl, pH 8.0, 50 mM KCl), 2.5 mM MgCl2 , 0.2 mM each deoxynucleoside triphosphate, 0.2 μM of each primer, and 1.5 units Multiplex Assay:Article Title: Influence of Antibiotic Selection on Genetic Composition of Escherichia coli Populations from Conventional and Organic Dairy Farms ▿ Populations from Conventional and Organic Dairy Farms ▿ † Article Snippet: Paragraph title: ECOR phylogrouping by multiplex PCR. ... Genomic DNA preparations were tested using primers targeting a 650-bp region of the conserved housekeeping gene mdh (see for primer sequences and reaction conditions), and Purification:Article Title: Priority Report: Rearrangements and Amplification of IER3 (IEX-1) Represent A Novel And Recurrent Molecular Abnormality In Myelodysplastic Syndromes (MDS) Article Snippet: To assess for point mutations in the propositus and other patients with MDS with and without 6p rearrangements, genomic DNA purified with QIAamp DNA Blood Mini Kit (QIAgen, Venlo, The Netherlands) was amplified by PCR on a PTC-200 Peltier thermocycler (MJ Research, Waltham, MA) and sequenced as for breakpoint cloning. .. Reagents included GeneAmp® PCR Buffer II (Applied Biosystems), 1.5 mM MgCl2 (Applied Biosystems), 200 μM dNTPs (Roche, Mannheim, Germany), forward and reverse oligonucleotide primers (see ), 100 ng of template DNA, and Article Title: The influence of FKBP5 genotype on expression of FKBP5 and other glucocorticoid-regulated genes, dependent on trauma exposure Article Snippet: To extract genomic DNA, briefly, cells were lysed in 1 % SDS, 0.05 M EDTA, 50 mM Tris–Cl, pH 8.0, and 100 ng/ml proteinase K, and incubated at 55 °C for 16 h. Genomic DNA was purified by phenol/chloroform extraction. .. PCR amplification with primers ( ) for FKBP5 was performed using Article Title: Oral microbiome diversity in chimpanzees from Gombe National Park Article Snippet: The solution was then purified again using a MinElute according to manufacturer protocol and eluted into 20 µL EB buffer. .. Following shotgun preparation, samples were amplified using Article Title: Mutations of RAS genes in endometrial polyps Article Snippet: The NRAS exon 2 was amplified from the extracted DNA by Applied Biosystems Article Title: Establishment of Murine Cytomegalovirus Latency In Vivo Is Associated with Changes in Histone Modifications and Recruitment of Transcriptional Repressors to the Major Immediate-Early Promoter ▿ Article Snippet: Prior to the addition of antibody to the sheared chromatin, an aliquot (subsequently referred to as the input) was removed and purified in a manner similar to that for the bound ChIP fraction. .. Semiquantitative PCR amplification of the MCMV MIEP segment was performed in the presence of 400 nM of each primer (Table ), 0.2 mM each of dATP, dCTP, dGTP, and dTTP, 1× PCR buffer, 1.5 mM MgCl2 , 1 unit of Article Title: History-driven population structure and asymmetric gene flow in a recovering large carnivore at the rear-edge of its European range Article Snippet: Amplifications were performed in 15-µl reactions containing 1x Gold Buffer (Applied Biosystems, Foster City, CA, USA), 2.5 mM MgCl2 , 0.2 mM dNTPs, 0.4 µM forward primers, 0.4 µM reverse primer and 0.027 U/µl Article Title: Novel TNXB Variants in Two Italian Patients with Classical-Like Ehlers-Danlos Syndrome Article Snippet: Reactions were performed in a 25 uL volume reaction containing 2.5 uL Buffer (10 ×), dNTP (0,25 mM final concentration), primers (10 pmol each), 1.25 U Polymerase Chain Reaction:Article Title: DNA Repair Gene Polymorphisms May Be Associated with Prognosis of Upper Urinary Tract Transitional Cell Carcinoma 1 Article Snippet: .. Briefly, the DNA fragments were amplified from 10 ng of DNA in 10 µl of PCR reactions containing 1.5 mM MgCl2 , 0.2 mM each deoxyribonucleotide triphosphate, 0.3 U of Article Title: Parathyroid tumor development involves deregulation of homeobox genes Article Snippet: .. Genomic DNA was amplified by PCR using the following conditions: 10 ng genomic DNA, 0.2 mM each primer, 200 mM each dNTP, 2 mM MgCl2 , 0.5 units Article Title: Priority Report: Rearrangements and Amplification of IER3 (IEX-1) Represent A Novel And Recurrent Molecular Abnormality In Myelodysplastic Syndromes (MDS) Article Snippet: .. Reagents included GeneAmp® PCR Buffer II (Applied Biosystems), 1.5 mM MgCl2 (Applied Biosystems), 200 μM dNTPs (Roche, Mannheim, Germany), forward and reverse oligonucleotide primers (see ), 100 ng of template DNA, and Article Title: The influence of FKBP5 genotype on expression of FKBP5 and other glucocorticoid-regulated genes, dependent on trauma exposure Article Snippet: .. PCR amplification with primers ( ) for FKBP5 was performed using Article Title: Influence of Antibiotic Selection on Genetic Composition of Escherichia coli Populations from Conventional and Organic Dairy Farms ▿ Populations from Conventional and Organic Dairy Farms ▿ † Article Snippet: Paragraph title: ECOR phylogrouping by multiplex PCR. ... Genomic DNA preparations were tested using primers targeting a 650-bp region of the conserved housekeeping gene mdh (see for primer sequences and reaction conditions), and Article Title: The emergence of a new strain of porcine circovirus-2 in Ontario and Quebec swine and its association with severe porcine circovirus associated disease -- 2004-2006 Article Snippet: .. The master mix was prepared in 50 μL final volume using 2.5 U Article Title: Oral microbiome diversity in chimpanzees from Gombe National Park Article Snippet: Following shotgun preparation, samples were amplified using Article Title: Hyperglycosylation and Reduced GABA Currents of Mutated GABRB3 Polypeptide in Remitting Childhood Absence Epilepsy Article Snippet: .. For PCR reactions, the Article Title: Mutations of RAS genes in endometrial polyps Article Snippet: The NRAS exon 2 was amplified from the extracted DNA by Applied Biosystems Article Title: Establishment of Murine Cytomegalovirus Latency In Vivo Is Associated with Changes in Histone Modifications and Recruitment of Transcriptional Repressors to the Major Immediate-Early Promoter ▿ Article Snippet: .. Semiquantitative PCR amplification of the MCMV MIEP segment was performed in the presence of 400 nM of each primer (Table ), 0.2 mM each of dATP, dCTP, dGTP, and dTTP, 1× PCR buffer, 1.5 mM MgCl2 , 1 unit of Article Title: Tumor suppressive BTB/POZ zinc-finger protein ZBTB28 inhibits oncogenic BCL6/ZBTB27 signaling to maintain p53 transcription in multiple carcinogenesis Article Snippet: Paragraph title: Bisulfite conversion and methylation-specific PCR (MSP) ... Article Title: TZAP Mutation Leads to Poor Prognosis of Patients with Breast Cancer † Article Snippet: .. PCR was performed using Article Title: Multilocus Variable-Number Tandem-Repeat Analysis for Investigation of Clostridium difficile Transmission in Hospitals Article Snippet: .. PCRs were performed in 50-μl volumes containing 1× AmpliTaq Gold PCR buffer (15 mM Tris-HCl, pH 8.0, 50 mM KCl), 2.5 mM MgCl2 , 0.2 mM each deoxynucleoside triphosphate, 0.2 μM of each primer, and 1.5 units Article Title: History-driven population structure and asymmetric gene flow in a recovering large carnivore at the rear-edge of its European range Article Snippet: PCR products were run in an automated sequencer (ABI 310) and genotypes were determined using ABI Genescan and Genotyper version 2.1 software. .. Amplifications were performed in 15-µl reactions containing 1x Gold Buffer (Applied Biosystems, Foster City, CA, USA), 2.5 mM MgCl2 , 0.2 mM dNTPs, 0.4 µM forward primers, 0.4 µM reverse primer and 0.027 U/µl Article Title: Novel TNXB Variants in Two Italian Patients with Classical-Like Ehlers-Danlos Syndrome Article Snippet: The 4987 bp PCR product was checked on ethidium bromide (EtBr) stained 1% agarose gel and then used as template for seven nested PCR amplifications with the primers listed in . .. Reactions were performed in a 25 uL volume reaction containing 2.5 uL Buffer (10 ×), dNTP (0,25 mM final concentration), primers (10 pmol each), 1.25 U Blocking Assay:Article Title: Mutations of RAS genes in endometrial polyps Article Snippet: Serial slices of 7 µm were prepared from a block. .. The NRAS exon 2 was amplified from the extracted DNA by Applied Biosystems T-Test:Article Title: The influence of FKBP5 genotype on expression of FKBP5 and other glucocorticoid-regulated genes, dependent on trauma exposure Article Snippet: PCR amplification with primers ( ) for FKBP5 was performed using Nested PCR:Article Title: Novel TNXB Variants in Two Italian Patients with Classical-Like Ehlers-Danlos Syndrome Article Snippet: The 4987 bp PCR product was checked on ethidium bromide (EtBr) stained 1% agarose gel and then used as template for seven nested PCR amplifications with the primers listed in . .. Reactions were performed in a 25 uL volume reaction containing 2.5 uL Buffer (10 ×), dNTP (0,25 mM final concentration), primers (10 pmol each), 1.25 U Chromatin Immunoprecipitation:Article Title: Establishment of Murine Cytomegalovirus Latency In Vivo Is Associated with Changes in Histone Modifications and Recruitment of Transcriptional Repressors to the Major Immediate-Early Promoter ▿ Article Snippet: Paragraph title: ChIP. ... Semiquantitative PCR amplification of the MCMV MIEP segment was performed in the presence of 400 nM of each primer (Table ), 0.2 mM each of dATP, dCTP, dGTP, and dTTP, 1× PCR buffer, 1.5 mM MgCl2 , 1 unit of Plasmid Preparation:Article Title: Mutations of RAS genes in endometrial polyps Article Snippet: The NRAS exon 2 was amplified from the extracted DNA by Applied Biosystems Software:Article Title: Parathyroid tumor development involves deregulation of homeobox genes Article Snippet: Genomic DNA was amplified by PCR using the following conditions: 10 ng genomic DNA, 0.2 mM each primer, 200 mM each dNTP, 2 mM MgCl2 , 0.5 units Article Title: Priority Report: Rearrangements and Amplification of IER3 (IEX-1) Represent A Novel And Recurrent Molecular Abnormality In Myelodysplastic Syndromes (MDS) Article Snippet: Amplicons detected by ethidium-bromide-impregnated agarose gel electrophoresis were then sequenced using fluorescent dye chemistry sequencing to explicitly define the rearrangement (ABI Prism 3730 DNA Analyzer; Applied Biosystems, Foster City, CA), with chromatograms compared to , and ( IER3 ) using Sequencher 4.5 software (Gene Codes, Ann Arbor, MI). .. Reagents included GeneAmp® PCR Buffer II (Applied Biosystems), 1.5 mM MgCl2 (Applied Biosystems), 200 μM dNTPs (Roche, Mannheim, Germany), forward and reverse oligonucleotide primers (see ), 100 ng of template DNA, and Article Title: The influence of FKBP5 genotype on expression of FKBP5 and other glucocorticoid-regulated genes, dependent on trauma exposure Article Snippet: PCR amplification with primers ( ) for FKBP5 was performed using Article Title: History-driven population structure and asymmetric gene flow in a recovering large carnivore at the rear-edge of its European range Article Snippet: PCR products were run in an automated sequencer (ABI 310) and genotypes were determined using ABI Genescan and Genotyper version 2.1 software. .. Amplifications were performed in 15-µl reactions containing 1x Gold Buffer (Applied Biosystems, Foster City, CA, USA), 2.5 mM MgCl2 , 0.2 mM dNTPs, 0.4 µM forward primers, 0.4 µM reverse primer and 0.027 U/µl Negative Control:Article Title: Establishment of Murine Cytomegalovirus Latency In Vivo Is Associated with Changes in Histone Modifications and Recruitment of Transcriptional Repressors to the Major Immediate-Early Promoter ▿ Article Snippet: Semiquantitative PCR amplification of the MCMV MIEP segment was performed in the presence of 400 nM of each primer (Table ), 0.2 mM each of dATP, dCTP, dGTP, and dTTP, 1× PCR buffer, 1.5 mM MgCl2 , 1 unit of Selection:Article Title: Mutations of RAS genes in endometrial polyps Article Snippet: The NRAS exon 2 was amplified from the extracted DNA by Applied Biosystems Agarose Gel Electrophoresis:Article Title: Priority Report: Rearrangements and Amplification of IER3 (IEX-1) Represent A Novel And Recurrent Molecular Abnormality In Myelodysplastic Syndromes (MDS) Article Snippet: Amplicons detected by ethidium-bromide-impregnated agarose gel electrophoresis were then sequenced using fluorescent dye chemistry sequencing to explicitly define the rearrangement (ABI Prism 3730 DNA Analyzer; Applied Biosystems, Foster City, CA), with chromatograms compared to , and ( IER3 ) using Sequencher 4.5 software (Gene Codes, Ann Arbor, MI). .. Reagents included GeneAmp® PCR Buffer II (Applied Biosystems), 1.5 mM MgCl2 (Applied Biosystems), 200 μM dNTPs (Roche, Mannheim, Germany), forward and reverse oligonucleotide primers (see ), 100 ng of template DNA, and Article Title: The emergence of a new strain of porcine circovirus-2 in Ontario and Quebec swine and its association with severe porcine circovirus associated disease -- 2004-2006 Article Snippet: The master mix was prepared in 50 μL final volume using 2.5 U Article Title: Establishment of Murine Cytomegalovirus Latency In Vivo Is Associated with Changes in Histone Modifications and Recruitment of Transcriptional Repressors to the Major Immediate-Early Promoter ▿ Article Snippet: Semiquantitative PCR amplification of the MCMV MIEP segment was performed in the presence of 400 nM of each primer (Table ), 0.2 mM each of dATP, dCTP, dGTP, and dTTP, 1× PCR buffer, 1.5 mM MgCl2 , 1 unit of Article Title: Tumor suppressive BTB/POZ zinc-finger protein ZBTB28 inhibits oncogenic BCL6/ZBTB27 signaling to maintain p53 transcription in multiple carcinogenesis Article Snippet: Article Title: TZAP Mutation Leads to Poor Prognosis of Patients with Breast Cancer † Article Snippet: PCR was performed using Article Title: Novel TNXB Variants in Two Italian Patients with Classical-Like Ehlers-Danlos Syndrome Article Snippet: The 4987 bp PCR product was checked on ethidium bromide (EtBr) stained 1% agarose gel and then used as template for seven nested PCR amplifications with the primers listed in . .. Reactions were performed in a 25 uL volume reaction containing 2.5 uL Buffer (10 ×), dNTP (0,25 mM final concentration), primers (10 pmol each), 1.25 U Laser Capture Microdissection:Article Title: Mutations of RAS genes in endometrial polyps Article Snippet: Paragraph title: Laser-capture microdissection and RAS-targeted sequencing ... The NRAS exon 2 was amplified from the extracted DNA by Applied Biosystems Spectrophotometry:Article Title: The influence of FKBP5 genotype on expression of FKBP5 and other glucocorticoid-regulated genes, dependent on trauma exposure Article Snippet: DNA quality and quantity was assessed using a NanoDrop 2000 Spectrophotometer (Thermo Scientific, Waltham, MA, USA). .. PCR amplification with primers ( ) for FKBP5 was performed using Article Title: Influence of Antibiotic Selection on Genetic Composition of Escherichia coli Populations from Conventional and Organic Dairy Farms ▿ Populations from Conventional and Organic Dairy Farms ▿ † Article Snippet: DNA preparations were quantified with a NanoDrop ND-1000 UV-visible spectrophotometer (NanoDrop Technologies, Wilmington, DE), diluted to a final concentration of 100 ng/μl, and stored at 4°C. .. Genomic DNA preparations were tested using primers targeting a 650-bp region of the conserved housekeeping gene mdh (see for primer sequences and reaction conditions), and Produced:Article Title: Influence of Antibiotic Selection on Genetic Composition of Escherichia coli Populations from Conventional and Organic Dairy Farms ▿ Populations from Conventional and Organic Dairy Farms ▿ † Article Snippet: Genomic DNA preparations were tested using primers targeting a 650-bp region of the conserved housekeeping gene mdh (see for primer sequences and reaction conditions), and Immunoprecipitation:Article Title: Establishment of Murine Cytomegalovirus Latency In Vivo Is Associated with Changes in Histone Modifications and Recruitment of Transcriptional Repressors to the Major Immediate-Early Promoter ▿ Article Snippet: Generally, one-third of the immunoprecipitated DNA was used for semiquantitative and/or real-time PCR amplification. .. Semiquantitative PCR amplification of the MCMV MIEP segment was performed in the presence of 400 nM of each primer (Table ), 0.2 mM each of dATP, dCTP, dGTP, and dTTP, 1× PCR buffer, 1.5 mM MgCl2 , 1 unit of Staining:Article Title: DNA Repair Gene Polymorphisms May Be Associated with Prognosis of Upper Urinary Tract Transitional Cell Carcinoma 1 Article Snippet: Briefly, the DNA fragments were amplified from 10 ng of DNA in 10 µl of PCR reactions containing 1.5 mM MgCl2 , 0.2 mM each deoxyribonucleotide triphosphate, 0.3 U of Article Title: The emergence of a new strain of porcine circovirus-2 in Ontario and Quebec swine and its association with severe porcine circovirus associated disease -- 2004-2006 Article Snippet: The master mix was prepared in 50 μL final volume using 2.5 U Article Title: Mutations of RAS genes in endometrial polyps Article Snippet: After deparaffinization with xylene, the tissue sections were stained with hematoxylin and eosin, and the glandular and stromal lesions were dissected by a PALM-IV system (Carl Zeiss Microscopy, Oberkochen, Germany). .. The NRAS exon 2 was amplified from the extracted DNA by Applied Biosystems Article Title: TZAP Mutation Leads to Poor Prognosis of Patients with Breast Cancer † Article Snippet: PCR was performed using Article Title: Novel TNXB Variants in Two Italian Patients with Classical-Like Ehlers-Danlos Syndrome Article Snippet: The 4987 bp PCR product was checked on ethidium bromide (EtBr) stained 1% agarose gel and then used as template for seven nested PCR amplifications with the primers listed in . .. Reactions were performed in a 25 uL volume reaction containing 2.5 uL Buffer (10 ×), dNTP (0,25 mM final concentration), primers (10 pmol each), 1.25 U Variant Assay:Article Title: DNA Repair Gene Polymorphisms May Be Associated with Prognosis of Upper Urinary Tract Transitional Cell Carcinoma 1 Article Snippet: Briefly, the DNA fragments were amplified from 10 ng of DNA in 10 µl of PCR reactions containing 1.5 mM MgCl2 , 0.2 mM each deoxyribonucleotide triphosphate, 0.3 U of |
