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Promega amplitaq gold dna polymerase
Amplitaq Gold Dna Polymerase, supplied by Promega, used in various techniques. Bioz Stars score: 92/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/amplitaq gold dna polymerase/product/Promega
Average 92 stars, based on 4 article reviews
Price from $9.99 to $1999.99
amplitaq gold dna polymerase - by Bioz Stars, 2020-09
92/100 stars

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Polymerase Chain Reaction:

Article Title: Proteolytic cleavage and truncation of NDRG1 in human prostate cancer cells, but not normal prostate epithelial cells
Article Snippet: .. Briefly, PCR was carried out using four sets of NDRG1 primers ( ) designed using the Primer3 website ( http://frodo.wi.mit.edu/primer3/ ) to amplify four distinct regions of NDRG1 by RT–PCR with the following cycle profile: one cycle of heat activation of the AmpliTaq Gold DNA polymerase (Promega) at 95°C for 1 min, then 39 cycles of 95°C for 30 s, 60°C for 30 s and 72°C for 30 s. The PCR was completed by one cycle at 72°C for 5 min. .. The PCR products were visualized on a 1% agarose gel by ethidium bromide.

Clone Assay:

Article Title: Novel animal models for studying complex brain disorders: BAC-driven miRNA-mediated in vivo silencing of gene expression
Article Snippet: .. These oligos were annealed and filled in using Amplitaq Gold DNA polymerase, digested with Not I and cloned into the pGEM 11ZF (Promega Corporation, Madison, WI, USA). .. After sequence verification, a minigene consisting of the 3′ portion of exon 1, the entire intron 1 and the 5′ portion of exon 2 of the human β-globin gene was amplified and cloned into the Spe I site of the Lox 2272 construct described above using Xba I sites engineered into the 5′ and 3′ ends of the β-globin cassette.

Amplification:

Article Title: Regulation of pregnane-X-receptor, CYP3A and P-glycoprotein genes in the PCB-resistant killifish (Fundulus heteroclitus) population from New Bedford Harbor 1
Article Snippet: .. Gut cDNA was amplified with the degenerate primers PX-F4 (5'-GGITACCACTTYAAYGC-3') and PX-R5 (5'-ACICCIGGICGRTCIGG-3') using AmpliTaq Gold DNA polymerase (Promega, Madison, WI, USA). ..

Activation Assay:

Article Title: Proteolytic cleavage and truncation of NDRG1 in human prostate cancer cells, but not normal prostate epithelial cells
Article Snippet: .. Briefly, PCR was carried out using four sets of NDRG1 primers ( ) designed using the Primer3 website ( http://frodo.wi.mit.edu/primer3/ ) to amplify four distinct regions of NDRG1 by RT–PCR with the following cycle profile: one cycle of heat activation of the AmpliTaq Gold DNA polymerase (Promega) at 95°C for 1 min, then 39 cycles of 95°C for 30 s, 60°C for 30 s and 72°C for 30 s. The PCR was completed by one cycle at 72°C for 5 min. .. The PCR products were visualized on a 1% agarose gel by ethidium bromide.

Reverse Transcription Polymerase Chain Reaction:

Article Title: Proteolytic cleavage and truncation of NDRG1 in human prostate cancer cells, but not normal prostate epithelial cells
Article Snippet: .. Briefly, PCR was carried out using four sets of NDRG1 primers ( ) designed using the Primer3 website ( http://frodo.wi.mit.edu/primer3/ ) to amplify four distinct regions of NDRG1 by RT–PCR with the following cycle profile: one cycle of heat activation of the AmpliTaq Gold DNA polymerase (Promega) at 95°C for 1 min, then 39 cycles of 95°C for 30 s, 60°C for 30 s and 72°C for 30 s. The PCR was completed by one cycle at 72°C for 5 min. .. The PCR products were visualized on a 1% agarose gel by ethidium bromide.

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  • 92
    Promega amplitaq gold
    T1942C PCR-SSCP. Three DNA samples, 1942T/T (homozygous normal), 1942C/C (homozygous polymorphic), 1942T/C (heterozygous), were amplified with <t>AmpliTaq</t> Gold polymerase and AmpliTaq Gold buffer ( A ), Promega Taq and Promega buffer ( B ), Promega Taq and AmpliTaq Gold buffer ( C ) and with AmpliTaq Gold and Promega buffer (no PCR products obtained). All samples were amplified and SSCP-electrophoresed in the same experiment. Arrows point to the polymorphic SSCP fragment. Note that the polymorphic fragments are clearly visible only after amplification in AmpliTaq Gold buffer ( A , C ).
    Amplitaq Gold, supplied by Promega, used in various techniques. Bioz Stars score: 92/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/amplitaq gold/product/Promega
    Average 92 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    amplitaq gold - by Bioz Stars, 2020-09
    92/100 stars
      Buy from Supplier

    91
    Promega amplitaq gold tm dna polymerase
    T1942C PCR-SSCP. Three DNA samples, 1942T/T (homozygous normal), 1942C/C (homozygous polymorphic), 1942T/C (heterozygous), were amplified with <t>AmpliTaq</t> Gold polymerase and AmpliTaq Gold buffer ( A ), Promega Taq and Promega buffer ( B ), Promega Taq and AmpliTaq Gold buffer ( C ) and with AmpliTaq Gold and Promega buffer (no PCR products obtained). All samples were amplified and SSCP-electrophoresed in the same experiment. Arrows point to the polymorphic SSCP fragment. Note that the polymorphic fragments are clearly visible only after amplification in AmpliTaq Gold buffer ( A , C ).
    Amplitaq Gold Tm Dna Polymerase, supplied by Promega, used in various techniques. Bioz Stars score: 91/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/amplitaq gold tm dna polymerase/product/Promega
    Average 91 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    amplitaq gold tm dna polymerase - by Bioz Stars, 2020-09
    91/100 stars
      Buy from Supplier

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    T1942C PCR-SSCP. Three DNA samples, 1942T/T (homozygous normal), 1942C/C (homozygous polymorphic), 1942T/C (heterozygous), were amplified with AmpliTaq Gold polymerase and AmpliTaq Gold buffer ( A ), Promega Taq and Promega buffer ( B ), Promega Taq and AmpliTaq Gold buffer ( C ) and with AmpliTaq Gold and Promega buffer (no PCR products obtained). All samples were amplified and SSCP-electrophoresed in the same experiment. Arrows point to the polymorphic SSCP fragment. Note that the polymorphic fragments are clearly visible only after amplification in AmpliTaq Gold buffer ( A , C ).

    Journal: British Journal of Cancer

    Article Title: Frequent loss of the AXIN1 locus but absence of AXIN1 gene mutations in adenocarcinomas of the gastro-oesophageal junction with nuclear β-catenin expression

    doi: 10.1038/sj.bjc.6601589

    Figure Lengend Snippet: T1942C PCR-SSCP. Three DNA samples, 1942T/T (homozygous normal), 1942C/C (homozygous polymorphic), 1942T/C (heterozygous), were amplified with AmpliTaq Gold polymerase and AmpliTaq Gold buffer ( A ), Promega Taq and Promega buffer ( B ), Promega Taq and AmpliTaq Gold buffer ( C ) and with AmpliTaq Gold and Promega buffer (no PCR products obtained). All samples were amplified and SSCP-electrophoresed in the same experiment. Arrows point to the polymorphic SSCP fragment. Note that the polymorphic fragments are clearly visible only after amplification in AmpliTaq Gold buffer ( A , C ).

    Article Snippet: To determine whether the SSCP conditions have influence on the detection of the T1942C SNP, we amplified DNA samples with AmpliTaq Gold and with Promega Taq DNA polymerases (Promega, Madison, WI, USA) in both AmpliTaq Gold and Promega Taq buffers.

    Techniques: Polymerase Chain Reaction, Amplification