amplitaq gold dna polymerase  (PerkinElmer)

 
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    SMARTintro Sample Introduction Module Gold with One Piece Quartz Torch Injector
    Description:
    This module incorporated in the NexION 1000 2000 ICP MS Geological Sample Introduction Kit N8150040 includes
    Catalog Number:
    N8152535
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    1638.0
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    PerkinElmer amplitaq gold dna polymerase
    SMARTintro Sample Introduction Module Gold with One Piece Quartz Torch Injector
    This module incorporated in the NexION 1000 2000 ICP MS Geological Sample Introduction Kit N8150040 includes
    https://www.bioz.com/result/amplitaq gold dna polymerase/product/PerkinElmer
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    amplitaq gold dna polymerase - by Bioz Stars, 2021-05
    93/100 stars

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    1) Product Images from "Colorectal papillomavirus infection in colorectal cancer patients"

    Article Title: Colorectal papillomavirus infection in colorectal cancer patients

    Journal: Clinical cancer research : an official journal of the American Association for Cancer Research

    doi: 10.1158/1078-0432.CCR-04-1680

    Detection of HPV16 DNA in colorectal tissues by in situ PCR. A, dark-blue nuclear staining indicates the many HPV16 DNA + tumor epithelial cells in a tumor lesion (x200). B, enlargement (x400) from (A) showing HPV16 DNA + tumor epithelial cells (arrows) and cells negative for nuclear staining (arrowheads) in tumor tissue. C, tumor tissue section with in situ PCR amplification in the absence of AmpliTaq Gold DNA Polymerase (x200). D, negative in situ PCR amplification of a tumor-adjacent, HPV - , normal colorectal tissue section (x200). No HPV16 DNA + cells are seen. The pattern seen in the normal tissue is missing from the tumor sections because of dysplasia and the angle at which the tissue was cut.
    Figure Legend Snippet: Detection of HPV16 DNA in colorectal tissues by in situ PCR. A, dark-blue nuclear staining indicates the many HPV16 DNA + tumor epithelial cells in a tumor lesion (x200). B, enlargement (x400) from (A) showing HPV16 DNA + tumor epithelial cells (arrows) and cells negative for nuclear staining (arrowheads) in tumor tissue. C, tumor tissue section with in situ PCR amplification in the absence of AmpliTaq Gold DNA Polymerase (x200). D, negative in situ PCR amplification of a tumor-adjacent, HPV - , normal colorectal tissue section (x200). No HPV16 DNA + cells are seen. The pattern seen in the normal tissue is missing from the tumor sections because of dysplasia and the angle at which the tissue was cut.

    Techniques Used: In Situ, Polymerase Chain Reaction, Staining, Amplification

    Related Articles

    Polymerase Chain Reaction:

    Article Title: Intragraft Selection of the T Cell Receptor Repertoire by Class I MHC Sequences in Tolerant Recipients
    Article Snippet: Spectrotyping of TCR Vβ CDR3 A 6-FAM-labeled internal primer within the Vβ PCR products (Cβ2 derived from β chain constant region) was synthesized by Applied Biosystems (Foster, CA). .. 7.5 µl of each Vβ-specific PCR product was subjected to 15 cycles of run-off reactions in a final volume of 10 µl, consisting of 0.25 rM of fluorochrome (FAM)-labeled internal primer Cβ 2, 0.2 mM of each dNTP, 2 mM MgCl2 , and 0.2 U AmpliTaq Gold (Perkin Elmer, Foster City, CA) in 1× PCR Gold buffer. .. Amplification was conducted in a GeneAmp PCR System 9700 (Perkin Elmer), with cycle conditions as follows: denaturation at 95°C for 10 min, 15 cycles of 30 sec at 95°C, 30 sec at 58°C, and 30 sec at 72°C, and elongation at 72°C for 5 min. At the end of the reaction, 5 µl of each spectrotyping reaction was mixed with 10 µl of GeneScan LIZ 500 Size Standard (Applied Biosystems , Foster, CA) in 96-well plate.

    Article Title: Colorectal papillomavirus infection in colorectal cancer patients
    Article Snippet: Each PCR reaction was carried out in a total volume of 50 μl containing 5 μl purified DNA, 1X AmpliTaq Gold PCR buffer (10 mM Tris-HCl [pH 8.3], 50 mM KCl), 2.5 mM MgCl2 , 200 μM of each dNTP, 100 nM of pooled PGMY09/11 primers, and 1.25 U of AmpliTaq Gold DNA polymerase (Perkin Elmer, Foster City, CA). .. PCR was carried out by activation of AmpliTaq Gold DNA polymerase for 9 min at 94°C and followed by 40 cycles of 30 sec each at 94°C, 55°C, and 72°C, with a final extension of 7 min at 72°C. .. One microliter of the first-run PCR reaction was used as a template for the nested PCR.

    Article Title: ?1B adrenergic receptors in gonadotrophin-releasing hormone neurones: relation to Transport-P
    Article Snippet: The reaction products were analysed in an ethidium bromide-stained agarose gel. .. In the PCRs which used the cDNA library as template, the reactions which used the A1BF4/A1BB4 and A1BF5/A1BB5 primer pairs consisted of PCR buffer (Perkin Elmer; 1×; MgCl2 1.5 m M ), dNTPs (200 μ M each), primers (1 μ M each), AmpliTaq Gold DNA polymerse (Perkin Elmer; 2.5 units) and template (0.5 μg) to a final volume of 75 μl. ..

    Article Title: Colorectal papillomavirus infection in colorectal cancer patients
    Article Snippet: This was followed by nested PCR using an internal primer set, GP5/6 ( , ). .. Each PCR reaction was carried out in a total volume of 50 μl containing 5 μl purified DNA, 1X AmpliTaq Gold PCR buffer (10 mM Tris-HCl [pH 8.3], 50 mM KCl), 2.5 mM MgCl2 , 200 μM of each dNTP, 100 nM of pooled PGMY09/11 primers, and 1.25 U of AmpliTaq Gold DNA polymerase (Perkin Elmer, Foster City, CA). .. PCR was carried out by activation of AmpliTaq Gold DNA polymerase for 9 min at 94°C and followed by 40 cycles of 30 sec each at 94°C, 55°C, and 72°C, with a final extension of 7 min at 72°C.

    Article Title: Effect of Nonionic Detergents on Amplification of Human Papillomavirus DNA with Consensus Primers MY09 and MY11
    Article Snippet: .. Ten-microliter volumes of the detergent preparations described below were added to 90-μl volumes of a PCR master mix containing the usual components ( , ) plus eight copies of HPV-16 (kindly provided by H. zur Hausen), 5 U of AmpliTaq Gold DNA polymerase (Perkin-Elmer, Montréal, Canada), and the L1 consensus HPV primers MY09 and MY11. .. Detergent solutions included (i) 10 μl of Tris-HCl (pH 7.4) with 0.01 mM EDTA (TE) (no-detergent control solution), (ii) 10 μl of a solution of 0.4% Tween 20 and 0.4% NP-40, (iii) 10 μl of a solution of 0.8% Tween 20, (iv) 10 μl of a solution of 0.8% NP-40, and (v) 10 μl of a solution of 0.8% Laureth 12 (kindly provided by P. Gravitt, Roche Molecular Systems).

    Article Title: Frequent loss of the AXIN1 locus but absence of AXIN1 gene mutations in adenocarcinomas of the gastro-oesophageal junction with nuclear β-catenin expression
    Article Snippet: The entire coding sequence, including the exon–intron boundaries, was investigated by PCR-SSCP using the previously described 23 sets of primers with slight modifications ( ). .. All amplifications were performed in 15 μ l PCR containing 50–100 ng DNA, 1.5 m MgCl2 , 0.02 m dATP, 0.2 m dGTP, dCTP and dTTP each, 0.8 μ Ci of [32 P]dATP (Amersham Biosciences, Buckinghamshire, UK), 20 pmol of each primer and 0.3 U AmpliTaq Gold polymerase (Perkin-Elmer Applied Biosystems, Foster City, CA, USA). .. AmpliTaq Gold polymerase was used because this enzyme is superior to other DNA polymerases with regard to amplification of DNA retrieved from routine formalin-fixed and paraffin-embedded tissues.

    Activation Assay:

    Article Title: Colorectal papillomavirus infection in colorectal cancer patients
    Article Snippet: Each PCR reaction was carried out in a total volume of 50 μl containing 5 μl purified DNA, 1X AmpliTaq Gold PCR buffer (10 mM Tris-HCl [pH 8.3], 50 mM KCl), 2.5 mM MgCl2 , 200 μM of each dNTP, 100 nM of pooled PGMY09/11 primers, and 1.25 U of AmpliTaq Gold DNA polymerase (Perkin Elmer, Foster City, CA). .. PCR was carried out by activation of AmpliTaq Gold DNA polymerase for 9 min at 94°C and followed by 40 cycles of 30 sec each at 94°C, 55°C, and 72°C, with a final extension of 7 min at 72°C. .. One microliter of the first-run PCR reaction was used as a template for the nested PCR.

    Size-exclusion Chromatography:

    Article Title: Colorectal papillomavirus infection in colorectal cancer patients
    Article Snippet: Each PCR reaction was carried out in a total volume of 50 μl containing 5 μl purified DNA, 1X AmpliTaq Gold PCR buffer (10 mM Tris-HCl [pH 8.3], 50 mM KCl), 2.5 mM MgCl2 , 200 μM of each dNTP, 100 nM of pooled PGMY09/11 primers, and 1.25 U of AmpliTaq Gold DNA polymerase (Perkin Elmer, Foster City, CA). .. PCR was carried out by activation of AmpliTaq Gold DNA polymerase for 9 min at 94°C and followed by 40 cycles of 30 sec each at 94°C, 55°C, and 72°C, with a final extension of 7 min at 72°C. .. One microliter of the first-run PCR reaction was used as a template for the nested PCR.

    cDNA Library Assay:

    Article Title: ?1B adrenergic receptors in gonadotrophin-releasing hormone neurones: relation to Transport-P
    Article Snippet: The reaction products were analysed in an ethidium bromide-stained agarose gel. .. In the PCRs which used the cDNA library as template, the reactions which used the A1BF4/A1BB4 and A1BF5/A1BB5 primer pairs consisted of PCR buffer (Perkin Elmer; 1×; MgCl2 1.5 m M ), dNTPs (200 μ M each), primers (1 μ M each), AmpliTaq Gold DNA polymerse (Perkin Elmer; 2.5 units) and template (0.5 μg) to a final volume of 75 μl. ..

    Purification:

    Article Title: Colorectal papillomavirus infection in colorectal cancer patients
    Article Snippet: This was followed by nested PCR using an internal primer set, GP5/6 ( , ). .. Each PCR reaction was carried out in a total volume of 50 μl containing 5 μl purified DNA, 1X AmpliTaq Gold PCR buffer (10 mM Tris-HCl [pH 8.3], 50 mM KCl), 2.5 mM MgCl2 , 200 μM of each dNTP, 100 nM of pooled PGMY09/11 primers, and 1.25 U of AmpliTaq Gold DNA polymerase (Perkin Elmer, Foster City, CA). .. PCR was carried out by activation of AmpliTaq Gold DNA polymerase for 9 min at 94°C and followed by 40 cycles of 30 sec each at 94°C, 55°C, and 72°C, with a final extension of 7 min at 72°C.

    Amplification:

    Article Title: Frequent loss of the AXIN1 locus but absence of AXIN1 gene mutations in adenocarcinomas of the gastro-oesophageal junction with nuclear β-catenin expression
    Article Snippet: All amplifications were performed in 15 μ l PCR containing 50–100 ng DNA, 1.5 m MgCl2 , 0.02 m dATP, 0.2 m dGTP, dCTP and dTTP each, 0.8 μ Ci of [32 P]dATP (Amersham Biosciences, Buckinghamshire, UK), 20 pmol of each primer and 0.3 U AmpliTaq Gold polymerase (Perkin-Elmer Applied Biosystems, Foster City, CA, USA). .. AmpliTaq Gold polymerase was used because this enzyme is superior to other DNA polymerases with regard to amplification of DNA retrieved from routine formalin-fixed and paraffin-embedded tissues. .. To the PCRs with primer sets 4, 10, 12, 13, 14, 15, 18, 21 and 23, DMSO (5%) was added to increase the amplification efficiency.

    Article Title: Sequences From First Settlers Reveal Rapid Evolution in Icelandic mtDNA Pool
    Article Snippet: PCR, Cloning and Sequencing in the Reykjavik Laboratories All PCR reactions were set up and sealed at the pre-PCR laboratory. .. In each reaction, 1 µl of extracted template DNA was subjected to 40 cycles of amplification in 25 µl volume containing 1 unit of Taq polymerase (Ampli Taq Gold, Perkin Elmer, Palo Alto, CA), 10× reaction buffer, 2.5 mM MgCl2 , 0.2 mM dNTPs, 12 mg/ml of BSA and 20 pmoles of each pair of primer. .. After a one-time preliminary 12 min activation of the enzyme at 95°C, each cycle consisted of 1 min steps, with denaturation at 95°C, annealing from 52 to 55.5°C (depending on the primer pair used, see and ) and extension at 72°C.

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    PerkinElmer amplitaq gold dna polymerase
    Detection of HPV16 <t>DNA</t> in colorectal tissues by in situ PCR. A, dark-blue nuclear staining indicates the many HPV16 DNA + tumor epithelial cells in a tumor lesion (x200). B, enlargement (x400) from (A) showing HPV16 DNA + tumor epithelial cells (arrows) and cells negative for nuclear staining (arrowheads) in tumor tissue. C, tumor tissue section with in situ PCR amplification in the absence of <t>AmpliTaq</t> Gold DNA Polymerase (x200). D, negative in situ PCR amplification of a tumor-adjacent, HPV - , normal colorectal tissue section (x200). No HPV16 DNA + cells are seen. The pattern seen in the normal tissue is missing from the tumor sections because of dysplasia and the angle at which the tissue was cut.
    Amplitaq Gold Dna Polymerase, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/amplitaq gold dna polymerase/product/PerkinElmer
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    amplitaq gold dna polymerase - by Bioz Stars, 2021-05
    93/100 stars
      Buy from Supplier

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    Detection of HPV16 DNA in colorectal tissues by in situ PCR. A, dark-blue nuclear staining indicates the many HPV16 DNA + tumor epithelial cells in a tumor lesion (x200). B, enlargement (x400) from (A) showing HPV16 DNA + tumor epithelial cells (arrows) and cells negative for nuclear staining (arrowheads) in tumor tissue. C, tumor tissue section with in situ PCR amplification in the absence of AmpliTaq Gold DNA Polymerase (x200). D, negative in situ PCR amplification of a tumor-adjacent, HPV - , normal colorectal tissue section (x200). No HPV16 DNA + cells are seen. The pattern seen in the normal tissue is missing from the tumor sections because of dysplasia and the angle at which the tissue was cut.

    Journal: Clinical cancer research : an official journal of the American Association for Cancer Research

    Article Title: Colorectal papillomavirus infection in colorectal cancer patients

    doi: 10.1158/1078-0432.CCR-04-1680

    Figure Lengend Snippet: Detection of HPV16 DNA in colorectal tissues by in situ PCR. A, dark-blue nuclear staining indicates the many HPV16 DNA + tumor epithelial cells in a tumor lesion (x200). B, enlargement (x400) from (A) showing HPV16 DNA + tumor epithelial cells (arrows) and cells negative for nuclear staining (arrowheads) in tumor tissue. C, tumor tissue section with in situ PCR amplification in the absence of AmpliTaq Gold DNA Polymerase (x200). D, negative in situ PCR amplification of a tumor-adjacent, HPV - , normal colorectal tissue section (x200). No HPV16 DNA + cells are seen. The pattern seen in the normal tissue is missing from the tumor sections because of dysplasia and the angle at which the tissue was cut.

    Article Snippet: PCR was carried out by activation of AmpliTaq Gold DNA polymerase for 9 min at 94°C and followed by 40 cycles of 30 sec each at 94°C, 55°C, and 72°C, with a final extension of 7 min at 72°C.

    Techniques: In Situ, Polymerase Chain Reaction, Staining, Amplification

    Amplification of eight copies of HPV-16 accomplished in the presence of TE buffer (lanes b, c, and d), 0.04% (final concentration [vol/vol]) NP-40 and 0.04% Tween (lanes e and f), 0.08% Tween 20 (lanes g and h), and 0.08% NP-40 (lanes i and j). The negative control is in lane a. Amplification was done with AmpliTaq Gold DNA polymerase in a model 9600 DNA thermal cycler. A 450-bp band is visible in positive reactions.

    Journal: Journal of Clinical Microbiology

    Article Title: Effect of Nonionic Detergents on Amplification of Human Papillomavirus DNA with Consensus Primers MY09 and MY11

    doi:

    Figure Lengend Snippet: Amplification of eight copies of HPV-16 accomplished in the presence of TE buffer (lanes b, c, and d), 0.04% (final concentration [vol/vol]) NP-40 and 0.04% Tween (lanes e and f), 0.08% Tween 20 (lanes g and h), and 0.08% NP-40 (lanes i and j). The negative control is in lane a. Amplification was done with AmpliTaq Gold DNA polymerase in a model 9600 DNA thermal cycler. A 450-bp band is visible in positive reactions.

    Article Snippet: Ten-microliter volumes of the detergent preparations described below were added to 90-μl volumes of a PCR master mix containing the usual components ( , ) plus eight copies of HPV-16 (kindly provided by H. zur Hausen), 5 U of AmpliTaq Gold DNA polymerase (Perkin-Elmer, Montréal, Canada), and the L1 consensus HPV primers MY09 and MY11.

    Techniques: Amplification, Concentration Assay, Negative Control