Structured Review

PerkinElmer amplitaq gold dna polymerase
Amplitaq Gold Dna Polymerase, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 99/100, based on 274 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/amplitaq gold dna polymerase/product/PerkinElmer
Average 99 stars, based on 274 article reviews
Price from $9.99 to $1999.99
amplitaq gold dna polymerase - by Bioz Stars, 2020-09
99/100 stars

Images

Related Articles

Incubation:

Article Title: Structural and Functional Studies of the Modulator NS9283 Reveal Agonist-like Mechanism of Action at α4β2 Nicotinic Acetylcholine Receptors *
Article Snippet: .. Filters containing protein and bound [3 H]epibatidine were individually incubated for at least 4 h with 3 ml of Ultima Gold (PerkinElmer Life Sciences). .. Radioactivity was then measured by liquid scintillation counting on a Tri-Carb counter (PerkinElmer Life Sciences), and IC50 values were determined by nonlinear regression using GraphPad Prism, with all points in one experiment determined in triplicate.

other:

Article Title: Comparison of the In Vivo Biotransformation of Two Emerging Estrogenic Contaminants, BP2 and BPS, in Zebrafish Embryos and Adults
Article Snippet: Flo-Scint II and Ultima Gold liquid scintillation cocktails were purchased from PerkinElmer Life and Analytical Sciences (Courtaboeuf, France).

Article Title: Human apolipoprotein E ɛ4 expression impairs cerebral vascularization and blood–brain barrier function in mice
Article Snippet: Tissue samples were digested in 1 mL of Solvable (Perkin-Elmer, Waltham, MA, USA) at 50°C overnight, and then cooled to room temperature and mixed with 9 mL of Ultima Gold scintillation cocktail (Perkin-Elmer).

Article Title: 6-mercaptopurine promotes energetic failure in proliferating T cells
Article Snippet: The radioactive CO2 was counted by using liquid scintillation (Ultima Gold, Perkin Elmer).

Radioactivity:

Article Title: Mnk2 and Mnk1 Are Essential for Constitutive and Inducible Phosphorylation of Eukaryotic Initiation Factor 4E but Not for Cell Growth or Development
Article Snippet: .. The radioactivity was measured by scintillation counting with Ultima Gold scintillation cocktail (PerkinElmer). .. The bicistronic translation reporter plasmids, pEF-FFL-IRES-SPL and pEF-SPL-IRES-FFL, were constructed by the sequential subcloning of firefly luciferase (FFL) cDNA from pGL3 (Promega), sea pansy luciferase (SPL, also called Renilla reniformis luciferase) cDNA from pRL-SV40 (Promega), and the internal ribosome entry site (IRES) sequence from pIRES2EGFP (BD Bioscience Clontech) into the pEF-BOS-EX plasmid, which carries the EF-1α enhancer/promoter as an expression driver.

Article Title: Alzheimer’s Disease Phenotype or Inflammatory Insult Does Not Alter Function of L-Type Amino Acid Transporter 1 in Mouse Blood-Brain Barrier and Primary Astrocytes
Article Snippet: .. The cell lysate was mixed with Ultima Gold scintillation cocktail (PerkinElmer, Inc., Waltham, MA, USA) and radioactivity was measured with MicroBeta liquid scintillation counter (Wallac Oy, Finland). ..

Article Title: Phosphorylation of the phytosulfokine peptide receptor PSKR1 controls receptor activity
Article Snippet: .. The kinase and MBP bands were excised from the dried gels, mixed with scintillation liquid (Ultima Gold, PerkinElmer), and the radioactivity was determined (Tri-Carb 2910 TR instrument, PerkinElmer) in counts per minute (cpm). .. The background signal of the gel was subtracted and activities were calculated as cpm ng–1 kinase protein for autophosphorylation and in cpm ng–1 MBP for transphosphorylation activity.

Article Title: Electrospun Produced 3D Matrices for Covering of Vascular Stents: Paclitaxel Release Depending on Fiber Structure and Composition of the External Environment
Article Snippet: .. Radioactivity of the preparation was measured on a Tri-Carb 2800 TR β-counter (PerkinElmer, Waltham, MA, USA) in a “ULTIMA GOLD LTT” scintillator (Perkin Elmer, Waltham, MA, USA). .. An aliquot of the sample (0.1 mL) was thoroughly mixed with a scintillator (0.9 mL), and radioactivity was measured at the same time after preparation of the mixture.

Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93
    PerkinElmer amplitaq gold dna polymerase
    Amplification of eight copies of HPV-16 accomplished in the presence of TE buffer (lanes b, c, and d), 0.04% (final concentration [vol/vol]) NP-40 and 0.04% Tween (lanes e and f), 0.08% Tween 20 (lanes g and h), and 0.08% NP-40 (lanes i and j). The negative control is in lane a. Amplification was done with <t>AmpliTaq</t> Gold <t>DNA</t> polymerase in a model 9600 DNA thermal cycler. A 450-bp band is visible in positive reactions.
    Amplitaq Gold Dna Polymerase, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 93/100, based on 274 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/amplitaq gold dna polymerase/product/PerkinElmer
    Average 93 stars, based on 274 article reviews
    Price from $9.99 to $1999.99
    amplitaq gold dna polymerase - by Bioz Stars, 2020-09
    93/100 stars
      Buy from Supplier

    Image Search Results


    Amplification of eight copies of HPV-16 accomplished in the presence of TE buffer (lanes b, c, and d), 0.04% (final concentration [vol/vol]) NP-40 and 0.04% Tween (lanes e and f), 0.08% Tween 20 (lanes g and h), and 0.08% NP-40 (lanes i and j). The negative control is in lane a. Amplification was done with AmpliTaq Gold DNA polymerase in a model 9600 DNA thermal cycler. A 450-bp band is visible in positive reactions.

    Journal: Journal of Clinical Microbiology

    Article Title: Effect of Nonionic Detergents on Amplification of Human Papillomavirus DNA with Consensus Primers MY09 and MY11

    doi:

    Figure Lengend Snippet: Amplification of eight copies of HPV-16 accomplished in the presence of TE buffer (lanes b, c, and d), 0.04% (final concentration [vol/vol]) NP-40 and 0.04% Tween (lanes e and f), 0.08% Tween 20 (lanes g and h), and 0.08% NP-40 (lanes i and j). The negative control is in lane a. Amplification was done with AmpliTaq Gold DNA polymerase in a model 9600 DNA thermal cycler. A 450-bp band is visible in positive reactions.

    Article Snippet: Ten-microliter volumes of the detergent preparations described below were added to 90-μl volumes of a PCR master mix containing the usual components ( , ) plus eight copies of HPV-16 (kindly provided by H. zur Hausen), 5 U of AmpliTaq Gold DNA polymerase (Perkin-Elmer, Montréal, Canada), and the L1 consensus HPV primers MY09 and MY11.

    Techniques: Amplification, Concentration Assay, Negative Control

    Detection of HPV16 DNA in colorectal tissues by in situ PCR. A, dark-blue nuclear staining indicates the many HPV16 DNA + tumor epithelial cells in a tumor lesion (x200). B, enlargement (x400) from (A) showing HPV16 DNA + tumor epithelial cells (arrows) and cells negative for nuclear staining (arrowheads) in tumor tissue. C, tumor tissue section with in situ PCR amplification in the absence of AmpliTaq Gold DNA Polymerase (x200). D, negative in situ PCR amplification of a tumor-adjacent, HPV - , normal colorectal tissue section (x200). No HPV16 DNA + cells are seen. The pattern seen in the normal tissue is missing from the tumor sections because of dysplasia and the angle at which the tissue was cut.

    Journal: Clinical cancer research : an official journal of the American Association for Cancer Research

    Article Title: Colorectal papillomavirus infection in colorectal cancer patients

    doi: 10.1158/1078-0432.CCR-04-1680

    Figure Lengend Snippet: Detection of HPV16 DNA in colorectal tissues by in situ PCR. A, dark-blue nuclear staining indicates the many HPV16 DNA + tumor epithelial cells in a tumor lesion (x200). B, enlargement (x400) from (A) showing HPV16 DNA + tumor epithelial cells (arrows) and cells negative for nuclear staining (arrowheads) in tumor tissue. C, tumor tissue section with in situ PCR amplification in the absence of AmpliTaq Gold DNA Polymerase (x200). D, negative in situ PCR amplification of a tumor-adjacent, HPV - , normal colorectal tissue section (x200). No HPV16 DNA + cells are seen. The pattern seen in the normal tissue is missing from the tumor sections because of dysplasia and the angle at which the tissue was cut.

    Article Snippet: PCR was carried out by activation of AmpliTaq Gold DNA polymerase for 9 min at 94°C and followed by 40 cycles of 30 sec each at 94°C, 55°C, and 72°C, with a final extension of 7 min at 72°C.

    Techniques: In Situ, Polymerase Chain Reaction, Staining, Amplification