amplitaq gold 360  (Thermo Fisher)


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    Name:
    AmpliTaq Gold 360 Buffer Kit
    Description:
    The AmpliTaq Gold 360 Buffer Kit is used in conjunction with AmpliTaq Gold 360 DNA Polymerase and is intended for those occasions when the quantity of reagents supplied with the enzyme are not sufficient The kit includes 1 5 mL each of 10X AmpliTaq Gold 360 Buffer 25 mM MgCl2 and 3360 GC Enhancer Typically 1 5 mL is sufficient for use with 250 U of enzyme
    Catalog Number:
    4398853
    Price:
    None
    Applications:
    Amplification of Bisulfite-Treated DNA|GC-Rich PCR|Hot Start PCR|PCR|PCR & Real-Time PCR|PCR Buffers, dNTPs, MgCl2 & General Reagents|RNAi, Epigenetics & Non-Coding RNA Research|Routine PCR|Methylation Analysis
    Category:
    Lab Reagents and Chemicals
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    Structured Review

    Thermo Fisher amplitaq gold 360
    The AmpliTaq Gold 360 Buffer Kit is used in conjunction with AmpliTaq Gold 360 DNA Polymerase and is intended for those occasions when the quantity of reagents supplied with the enzyme are not sufficient The kit includes 1 5 mL each of 10X AmpliTaq Gold 360 Buffer 25 mM MgCl2 and 3360 GC Enhancer Typically 1 5 mL is sufficient for use with 250 U of enzyme
    https://www.bioz.com/result/amplitaq gold 360/product/Thermo Fisher
    Average 99 stars, based on 59 article reviews
    Price from $9.99 to $1999.99
    amplitaq gold 360 - by Bioz Stars, 2020-09
    99/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: Distinct microbial communities alter litter decomposition rates in a fertilized coastal plain wetland
    Article Snippet: .. For each sample, three 50 μL PCR libraries were prepared by combining 35.75 μL molecular grade water, 5 μL Amplitaq Gold 360 10x buffer, 5 μL MgCl2 (25 mM), 1 μL dNTPs (40mM total, 10mM each), 0.25 μL Amplitaq Gold polymerase, 1 μL 515 forward barcoded primer (10 μM), 1 μL 806 reverse primer (10 μM), and 1 μL DNA template (10 ng/μL). .. Thermocycler conditions for PCR reactions were as follows: initial denaturation (94 °C, 3 minutes); 30 cycles of 94 °C for 45 seconds, 50 °C for 30 seconds, 72 °C for 90 seconds; final elongation (72 °C, 10 minutes).

    Article Title: Significance of Compression in Binucleation while Differentiating Reactive Cellular Changes Between Human Papillomavirus and Candida Infections
    Article Snippet: .. The PCR reaction mixture included 1× AmpliTaq Gold® 360 buffer, 2 mM MgCl2 , 0.025 U/µl AmpliTaq Gold 360 DNA Polymerase (Applied Biosystems, CA, USA), 1 μl DNA, and 0.5 pM primers (HPV 16, 18, 31, 33, 35, 39, 45, 51, 52, 53, 56, 58, 59, 68) in a total volume of 25 μl. .. PCR amplification was performed using a thermal cycler with 35 cycles of denaturation at 95°C for 30 s, annealing at 60°C for 30 s, and extension at 72°C for 30 s, including an initial denaturation step of 10 min and a final extension step of 5 min. Human β-actin expression, determined using an additional PCR, was used as an internal standard; the resulting amplicon was 262-bp long.

    Article Title: Significance of molecular classification of ependymomas: C11orf95-RELA fusion-negative supratentorial ependymomas are a heterogeneous group of tumors
    Article Snippet: .. PCR and RT-PCR were performed using an AmpliTaq Gold 360 kit (Applied Biosystems, Foster City, CA, USA). .. Following purification with ExoSAP-IT (Affymetrix USB, Cleveland, OH, USA), Sanger sequencing was performed using a BigDye Terminator v1.1 Cycle Sequencing Kit (Applied Biosystems, Foster City, CA, USA) for screening TERT C228T and C250T mutations, and a BigDye Terminator v3.1 Cycle Sequencing Kit (Applied Biosystems, Foster City, CA, USA) for screening other genes on an auto sequencer (3130xl Genetic Analyzer, Applied Biosystems, Foster City, CA) in the sequencing analysis.

    Article Title: Urine as a high-quality source of host genomic DNA from wild populations
    Article Snippet: .. We incubated the solution at 37°C for 20 min followed by 80°C for 20 min. We amplified shotgun libraries using Amplitaq Gold before splitting libraries into four identical PCR reactions which contained 9.0 μL of DNA, 9.27 μL PCR Buffer II (10x), 9.27 μL MgCl2 (25mM), 3.68 μL dNTP mix (10nM), 2.21 μL BSA (10 mg/mL), 2.0 μL P5 primer, 2.0 μL P7 primer, 61.09 μL of ddH2 O, and 1.48 μL of Amplitaq Gold enzyme. .. We used the following PCR conditions: initial denaturation at 95°C for 15 min, followed by cycling of 95°C for 30 sec, 58°C for 30 sec, and 72°C for 45 sec, with a final elongation of 72°C for ten min. We amplified each sample between 8 and 13 cycles (Table S1) using Illumina adapter primers with unique forward and reverse barcodes.

    Article Title: Epigenetic changes in the CDKN2A locus are associated with differential expression of P16INK4A and P14ARF in HPV-positive oropharyngeal squamous cell carcinoma
    Article Snippet: .. The targeted PCR products were amplified using the Amplitaq Gold 360 Polymerase Kit from Applied Biosystems and sequenced by Albert Einstein College of Medicine Genomics Core. ..

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Significance of molecular classification of ependymomas: C11orf95-RELA fusion-negative supratentorial ependymomas are a heterogeneous group of tumors
    Article Snippet: .. PCR and RT-PCR were performed using an AmpliTaq Gold 360 kit (Applied Biosystems, Foster City, CA, USA). .. Following purification with ExoSAP-IT (Affymetrix USB, Cleveland, OH, USA), Sanger sequencing was performed using a BigDye Terminator v1.1 Cycle Sequencing Kit (Applied Biosystems, Foster City, CA, USA) for screening TERT C228T and C250T mutations, and a BigDye Terminator v3.1 Cycle Sequencing Kit (Applied Biosystems, Foster City, CA, USA) for screening other genes on an auto sequencer (3130xl Genetic Analyzer, Applied Biosystems, Foster City, CA) in the sequencing analysis.

    Incubation:

    Article Title: Urine as a high-quality source of host genomic DNA from wild populations
    Article Snippet: .. We incubated the solution at 37°C for 20 min followed by 80°C for 20 min. We amplified shotgun libraries using Amplitaq Gold before splitting libraries into four identical PCR reactions which contained 9.0 μL of DNA, 9.27 μL PCR Buffer II (10x), 9.27 μL MgCl2 (25mM), 3.68 μL dNTP mix (10nM), 2.21 μL BSA (10 mg/mL), 2.0 μL P5 primer, 2.0 μL P7 primer, 61.09 μL of ddH2 O, and 1.48 μL of Amplitaq Gold enzyme. .. We used the following PCR conditions: initial denaturation at 95°C for 15 min, followed by cycling of 95°C for 30 sec, 58°C for 30 sec, and 72°C for 45 sec, with a final elongation of 72°C for ten min. We amplified each sample between 8 and 13 cycles (Table S1) using Illumina adapter primers with unique forward and reverse barcodes.

    Amplification:

    Article Title: Urine as a high-quality source of host genomic DNA from wild populations
    Article Snippet: .. We incubated the solution at 37°C for 20 min followed by 80°C for 20 min. We amplified shotgun libraries using Amplitaq Gold before splitting libraries into four identical PCR reactions which contained 9.0 μL of DNA, 9.27 μL PCR Buffer II (10x), 9.27 μL MgCl2 (25mM), 3.68 μL dNTP mix (10nM), 2.21 μL BSA (10 mg/mL), 2.0 μL P5 primer, 2.0 μL P7 primer, 61.09 μL of ddH2 O, and 1.48 μL of Amplitaq Gold enzyme. .. We used the following PCR conditions: initial denaturation at 95°C for 15 min, followed by cycling of 95°C for 30 sec, 58°C for 30 sec, and 72°C for 45 sec, with a final elongation of 72°C for ten min. We amplified each sample between 8 and 13 cycles (Table S1) using Illumina adapter primers with unique forward and reverse barcodes.

    Article Title: Epigenetic changes in the CDKN2A locus are associated with differential expression of P16INK4A and P14ARF in HPV-positive oropharyngeal squamous cell carcinoma
    Article Snippet: .. The targeted PCR products were amplified using the Amplitaq Gold 360 Polymerase Kit from Applied Biosystems and sequenced by Albert Einstein College of Medicine Genomics Core. ..

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  • 99
    Thermo Fisher amplitaq gold 360 master mix
    Impact evaluation of Master Mix used in singleplex PCR. In graph: Elizyme = 2X EliZyme HS Robust MIX (Elisabeth Pharmacon, Czech Republic); Accustart II = AccuStart II PCR ToughMix (QuantaBio, Massachusetts, USA); <t>Amplitaq</t> = AmpliTaq <t>Gold</t> 360 Master Mix (Thermo Fisher Scientific, Massachusetts, USA); OneTaq = OneTaq Hot Start 2X Master Mix with GC Buffer (New England BioLabs, Massachusetts, USA); Platinum = Platinum Hot Start PCR 2X Master Mix (Invitrogen, California, USA); HotStarTaq = HotStarTaq DNA Polymerase (Qiagen, Germany). YE = Y . enterocolitica; TG = T . gondii ; plus sign in legend = positive sample; minus sign = NTC.
    Amplitaq Gold 360 Master Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 360 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/amplitaq gold 360 master mix/product/Thermo Fisher
    Average 99 stars, based on 360 article reviews
    Price from $9.99 to $1999.99
    amplitaq gold 360 master mix - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    89
    Thermo Fisher amplitaq gold 360 dna polymerase
    Impact evaluation of Master Mix used in singleplex PCR. In graph: Elizyme = 2X EliZyme HS Robust MIX (Elisabeth Pharmacon, Czech Republic); Accustart II = AccuStart II PCR ToughMix (QuantaBio, Massachusetts, USA); <t>Amplitaq</t> = AmpliTaq <t>Gold</t> 360 Master Mix (Thermo Fisher Scientific, Massachusetts, USA); OneTaq = OneTaq Hot Start 2X Master Mix with GC Buffer (New England BioLabs, Massachusetts, USA); Platinum = Platinum Hot Start PCR 2X Master Mix (Invitrogen, California, USA); HotStarTaq = HotStarTaq DNA Polymerase (Qiagen, Germany). YE = Y . enterocolitica; TG = T . gondii ; plus sign in legend = positive sample; minus sign = NTC.
    Amplitaq Gold 360 Dna Polymerase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 89/100, based on 68 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/amplitaq gold 360 dna polymerase/product/Thermo Fisher
    Average 89 stars, based on 68 article reviews
    Price from $9.99 to $1999.99
    amplitaq gold 360 dna polymerase - by Bioz Stars, 2020-09
    89/100 stars
      Buy from Supplier

    Image Search Results


    Impact evaluation of Master Mix used in singleplex PCR. In graph: Elizyme = 2X EliZyme HS Robust MIX (Elisabeth Pharmacon, Czech Republic); Accustart II = AccuStart II PCR ToughMix (QuantaBio, Massachusetts, USA); Amplitaq = AmpliTaq Gold 360 Master Mix (Thermo Fisher Scientific, Massachusetts, USA); OneTaq = OneTaq Hot Start 2X Master Mix with GC Buffer (New England BioLabs, Massachusetts, USA); Platinum = Platinum Hot Start PCR 2X Master Mix (Invitrogen, California, USA); HotStarTaq = HotStarTaq DNA Polymerase (Qiagen, Germany). YE = Y . enterocolitica; TG = T . gondii ; plus sign in legend = positive sample; minus sign = NTC.

    Journal: Scientific Reports

    Article Title: A novel perspective on MOL-PCR optimization and MAGPIX analysis of in-house multiplex foodborne pathogens detection assay

    doi: 10.1038/s41598-019-40035-5

    Figure Lengend Snippet: Impact evaluation of Master Mix used in singleplex PCR. In graph: Elizyme = 2X EliZyme HS Robust MIX (Elisabeth Pharmacon, Czech Republic); Accustart II = AccuStart II PCR ToughMix (QuantaBio, Massachusetts, USA); Amplitaq = AmpliTaq Gold 360 Master Mix (Thermo Fisher Scientific, Massachusetts, USA); OneTaq = OneTaq Hot Start 2X Master Mix with GC Buffer (New England BioLabs, Massachusetts, USA); Platinum = Platinum Hot Start PCR 2X Master Mix (Invitrogen, California, USA); HotStarTaq = HotStarTaq DNA Polymerase (Qiagen, Germany). YE = Y . enterocolitica; TG = T . gondii ; plus sign in legend = positive sample; minus sign = NTC.

    Article Snippet: In order to find a balance, the performance of six different master mixes was tested (Fig. ), including the previously utilized HotStarTaq DNA Polymerase – and the upgraded AmpliTaq Gold 360 Master Mix , .

    Techniques: Polymerase Chain Reaction, Hot Start PCR