amplitaq gold 360 master mix  (Thermo Fisher)


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    Name:
    AmpliTaq Gold 360 Master Mix
    Description:
    AmpliTaq Gold 360 PCR Master Mix contains everything required for successful PCR amplification in one convenient package all components are premixed and premeasured AmpliTaq Gold 360 Master Mix was designed for 360° coverage of a full range of targets The master mix is supplied at 2X the recommended usage concentration for easy dilution when adding template and primers Designed for convenience the AmpliTaq Gold 360 PCR Master Mix scales to various reaction volumes for greater application and format flexibility The main ingredient of the master mix is AmpliTaq Gold 360 DNA Polymerase but it also contains the world class 360 GC Enhancer which can be added optionally for high GC content templates With the wide template coverage optimization of PCR reaction conditions is virtually eliminated with the use of AmpliTaq Gold 360 Master Mix Key features • Optimized for the broadest range of targets from everyday to challenging• Unmatched sensitivity specificity and yield• Robust amplification of GC rich sequences with market leading 360 GC Enhancer• Achieves the highest quality sequencing data• Easy to use premixed master mixOptimized for easy and challenging targetsChallenging targets include AT rich GC rich primer dimer forming amplicons homopolymer repeats and amplicons that pose sequencing challenges Amplicons that previously required specialized enzymes and reaction conditions can now be reproducibly amplified with a single reagent under standardized conditions see figure Competitive benchmarking across more than 40 amplicons distinguishes AmpliTaq Gold 360 as the best performing enzyme ensuring the highest probability of success for the amplification of both everyday and challenging targets see table Optimized for automated hot start PCRAmpliTaq Gold 360 DNA Polymerase is the key ingredient in an automated convenient and efficient hot start PCR When AmpliTaq Gold 360 PCR Master Mix is added to the reaction mixture at room temperature the inactive enzyme is not capable of primer extension Any low stringency mispriming events that may have occurred will not be enzymatically extended and subsequently amplified An initial thermal incubation step is required for activation and ensures that active enzyme is generated only at temperatures where the DNA is fully denatured The amount of AmpliTaq Gold 360 DNA polymerase increases in the reaction slowly with each cycle number and specific product yield increases without buildup of nonspecific products including primer dimers Excellent specificity across a broad range of targets see figure The extreme specificity allows easier multiplexing and allelic discrimination Amplify low copy amplicons and long targetsAmpliTaq Gold 360 DNA Polymerase efficiently amplifies targets present at low copy number see figure even in the presence of high concentrations of complex DNA making it especially suited for low copy pathogen detection and amplification of targets from degraded DNA samples The extreme purity of the enzyme contributes to its unmatched sensitivity AmpliTaq Gold 360 DNA Polymerase efficiently and reproducibly amplifies long up to 5 kb sequences The figure shown demonstrates robust PCR amplification of long human and plasmid DNA
    Catalog Number:
    4398876
    Price:
    None
    Applications:
    Amplification of Bisulfite-Treated DNA|GC-Rich PCR|Hot Start PCR|Methylation-Sensitive Mobility Shift Assay|PCR|PCR & Real-Time PCR|PCR Enzymes & Master Mixes|RNAi, Epigenetics & Non-Coding RNA Research|Routine PCR|Methylation Analysis
    Category:
    Proteins Enzymes Peptides
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    Structured Review

    Thermo Fisher amplitaq gold 360 master mix
    Effects of different concentrations and sizes of UCNPs on PCR amplification specificity. Three commercial real-time PCR master mixes were applied to determine the effects of different concentrations and sizes of UCNPs on the specificity of PCR amplification of a 120 bp 5S rDNA fragment from soybean genomic DNA, at an annealing temperature of 50°C. ( A ) AccuPower PCR PreMix with Top Polymerase (Bioneer). ( B ) <t>AmpliTaq</t> <t>Gold</t> 360 Master Mix with AmpliTaq Gold DNA Polymerase (Applied Biosystems). ( C ) HotStarTaq Plus Master Mix with HotStarTaq DNA Polymerase (Qiagen). Lane M: DNA marker; lane C: template without UCNPs; lanes 1∼5∶5, 7.5, 10, 15, and 30 nM of QDs, respectively; lanes 6∼8∶0.5, 0.75, and 1.0 µg/µL of 40-nm-sized UCNPs, respectively; lanes 9∼14∶1×( = 2.4×10 5 particles/µL), 10×, 15×, 20×, 25×, and 30× of 70-nm-sized UCNPs, respectively; lanes 15∼20∶1×, 10×, 15×, 20×, 25×, and 30× of 250-nm-sized UCNPs, respectively.
    AmpliTaq Gold 360 PCR Master Mix contains everything required for successful PCR amplification in one convenient package all components are premixed and premeasured AmpliTaq Gold 360 Master Mix was designed for 360° coverage of a full range of targets The master mix is supplied at 2X the recommended usage concentration for easy dilution when adding template and primers Designed for convenience the AmpliTaq Gold 360 PCR Master Mix scales to various reaction volumes for greater application and format flexibility The main ingredient of the master mix is AmpliTaq Gold 360 DNA Polymerase but it also contains the world class 360 GC Enhancer which can be added optionally for high GC content templates With the wide template coverage optimization of PCR reaction conditions is virtually eliminated with the use of AmpliTaq Gold 360 Master Mix Key features • Optimized for the broadest range of targets from everyday to challenging• Unmatched sensitivity specificity and yield• Robust amplification of GC rich sequences with market leading 360 GC Enhancer• Achieves the highest quality sequencing data• Easy to use premixed master mixOptimized for easy and challenging targetsChallenging targets include AT rich GC rich primer dimer forming amplicons homopolymer repeats and amplicons that pose sequencing challenges Amplicons that previously required specialized enzymes and reaction conditions can now be reproducibly amplified with a single reagent under standardized conditions see figure Competitive benchmarking across more than 40 amplicons distinguishes AmpliTaq Gold 360 as the best performing enzyme ensuring the highest probability of success for the amplification of both everyday and challenging targets see table Optimized for automated hot start PCRAmpliTaq Gold 360 DNA Polymerase is the key ingredient in an automated convenient and efficient hot start PCR When AmpliTaq Gold 360 PCR Master Mix is added to the reaction mixture at room temperature the inactive enzyme is not capable of primer extension Any low stringency mispriming events that may have occurred will not be enzymatically extended and subsequently amplified An initial thermal incubation step is required for activation and ensures that active enzyme is generated only at temperatures where the DNA is fully denatured The amount of AmpliTaq Gold 360 DNA polymerase increases in the reaction slowly with each cycle number and specific product yield increases without buildup of nonspecific products including primer dimers Excellent specificity across a broad range of targets see figure The extreme specificity allows easier multiplexing and allelic discrimination Amplify low copy amplicons and long targetsAmpliTaq Gold 360 DNA Polymerase efficiently amplifies targets present at low copy number see figure even in the presence of high concentrations of complex DNA making it especially suited for low copy pathogen detection and amplification of targets from degraded DNA samples The extreme purity of the enzyme contributes to its unmatched sensitivity AmpliTaq Gold 360 DNA Polymerase efficiently and reproducibly amplifies long up to 5 kb sequences The figure shown demonstrates robust PCR amplification of long human and plasmid DNA
    https://www.bioz.com/result/amplitaq gold 360 master mix/product/Thermo Fisher
    Average 99 stars, based on 355 article reviews
    Price from $9.99 to $1999.99
    amplitaq gold 360 master mix - by Bioz Stars, 2020-07
    99/100 stars

    Images

    1) Product Images from "Effects of Upconversion Nanoparticles on Polymerase Chain Reaction"

    Article Title: Effects of Upconversion Nanoparticles on Polymerase Chain Reaction

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0073408

    Effects of different concentrations and sizes of UCNPs on PCR amplification specificity. Three commercial real-time PCR master mixes were applied to determine the effects of different concentrations and sizes of UCNPs on the specificity of PCR amplification of a 120 bp 5S rDNA fragment from soybean genomic DNA, at an annealing temperature of 50°C. ( A ) AccuPower PCR PreMix with Top Polymerase (Bioneer). ( B ) AmpliTaq Gold 360 Master Mix with AmpliTaq Gold DNA Polymerase (Applied Biosystems). ( C ) HotStarTaq Plus Master Mix with HotStarTaq DNA Polymerase (Qiagen). Lane M: DNA marker; lane C: template without UCNPs; lanes 1∼5∶5, 7.5, 10, 15, and 30 nM of QDs, respectively; lanes 6∼8∶0.5, 0.75, and 1.0 µg/µL of 40-nm-sized UCNPs, respectively; lanes 9∼14∶1×( = 2.4×10 5 particles/µL), 10×, 15×, 20×, 25×, and 30× of 70-nm-sized UCNPs, respectively; lanes 15∼20∶1×, 10×, 15×, 20×, 25×, and 30× of 250-nm-sized UCNPs, respectively.
    Figure Legend Snippet: Effects of different concentrations and sizes of UCNPs on PCR amplification specificity. Three commercial real-time PCR master mixes were applied to determine the effects of different concentrations and sizes of UCNPs on the specificity of PCR amplification of a 120 bp 5S rDNA fragment from soybean genomic DNA, at an annealing temperature of 50°C. ( A ) AccuPower PCR PreMix with Top Polymerase (Bioneer). ( B ) AmpliTaq Gold 360 Master Mix with AmpliTaq Gold DNA Polymerase (Applied Biosystems). ( C ) HotStarTaq Plus Master Mix with HotStarTaq DNA Polymerase (Qiagen). Lane M: DNA marker; lane C: template without UCNPs; lanes 1∼5∶5, 7.5, 10, 15, and 30 nM of QDs, respectively; lanes 6∼8∶0.5, 0.75, and 1.0 µg/µL of 40-nm-sized UCNPs, respectively; lanes 9∼14∶1×( = 2.4×10 5 particles/µL), 10×, 15×, 20×, 25×, and 30× of 70-nm-sized UCNPs, respectively; lanes 15∼20∶1×, 10×, 15×, 20×, 25×, and 30× of 250-nm-sized UCNPs, respectively.

    Techniques Used: Polymerase Chain Reaction, Amplification, Real-time Polymerase Chain Reaction, Marker

    2) Product Images from "A novel perspective on MOL-PCR optimization and MAGPIX analysis of in-house multiplex foodborne pathogens detection assay"

    Article Title: A novel perspective on MOL-PCR optimization and MAGPIX analysis of in-house multiplex foodborne pathogens detection assay

    Journal: Scientific Reports

    doi: 10.1038/s41598-019-40035-5

    Impact evaluation of Master Mix used in singleplex PCR. In graph: Elizyme = 2X EliZyme HS Robust MIX (Elisabeth Pharmacon, Czech Republic); Accustart II = AccuStart II PCR ToughMix (QuantaBio, Massachusetts, USA); Amplitaq = AmpliTaq Gold 360 Master Mix (Thermo Fisher Scientific, Massachusetts, USA); OneTaq = OneTaq Hot Start 2X Master Mix with GC Buffer (New England BioLabs, Massachusetts, USA); Platinum = Platinum Hot Start PCR 2X Master Mix (Invitrogen, California, USA); HotStarTaq = HotStarTaq DNA Polymerase (Qiagen, Germany). YE = Y . enterocolitica; TG = T . gondii ; plus sign in legend = positive sample; minus sign = NTC.
    Figure Legend Snippet: Impact evaluation of Master Mix used in singleplex PCR. In graph: Elizyme = 2X EliZyme HS Robust MIX (Elisabeth Pharmacon, Czech Republic); Accustart II = AccuStart II PCR ToughMix (QuantaBio, Massachusetts, USA); Amplitaq = AmpliTaq Gold 360 Master Mix (Thermo Fisher Scientific, Massachusetts, USA); OneTaq = OneTaq Hot Start 2X Master Mix with GC Buffer (New England BioLabs, Massachusetts, USA); Platinum = Platinum Hot Start PCR 2X Master Mix (Invitrogen, California, USA); HotStarTaq = HotStarTaq DNA Polymerase (Qiagen, Germany). YE = Y . enterocolitica; TG = T . gondii ; plus sign in legend = positive sample; minus sign = NTC.

    Techniques Used: Polymerase Chain Reaction, Hot Start PCR

    3) Product Images from "A novel perspective on MOL-PCR optimization and MAGPIX analysis of in-house multiplex foodborne pathogens detection assay"

    Article Title: A novel perspective on MOL-PCR optimization and MAGPIX analysis of in-house multiplex foodborne pathogens detection assay

    Journal: Scientific Reports

    doi: 10.1038/s41598-019-40035-5

    Impact evaluation of Master Mix used in singleplex PCR. In graph: Elizyme = 2X EliZyme HS Robust MIX (Elisabeth Pharmacon, Czech Republic); Accustart II = AccuStart II PCR ToughMix (QuantaBio, Massachusetts, USA); Amplitaq = AmpliTaq Gold 360 Master Mix (Thermo Fisher Scientific, Massachusetts, USA); OneTaq = OneTaq Hot Start 2X Master Mix with GC Buffer (New England BioLabs, Massachusetts, USA); Platinum = Platinum Hot Start PCR 2X Master Mix (Invitrogen, California, USA); HotStarTaq = HotStarTaq DNA Polymerase (Qiagen, Germany). YE = Y . enterocolitica; TG = T . gondii ; plus sign in legend = positive sample; minus sign = NTC.
    Figure Legend Snippet: Impact evaluation of Master Mix used in singleplex PCR. In graph: Elizyme = 2X EliZyme HS Robust MIX (Elisabeth Pharmacon, Czech Republic); Accustart II = AccuStart II PCR ToughMix (QuantaBio, Massachusetts, USA); Amplitaq = AmpliTaq Gold 360 Master Mix (Thermo Fisher Scientific, Massachusetts, USA); OneTaq = OneTaq Hot Start 2X Master Mix with GC Buffer (New England BioLabs, Massachusetts, USA); Platinum = Platinum Hot Start PCR 2X Master Mix (Invitrogen, California, USA); HotStarTaq = HotStarTaq DNA Polymerase (Qiagen, Germany). YE = Y . enterocolitica; TG = T . gondii ; plus sign in legend = positive sample; minus sign = NTC.

    Techniques Used: Polymerase Chain Reaction, Hot Start PCR

    Related Articles

    Polymerase Chain Reaction:

    Article Title: Insectivorous bats selectively source moths and eat mostly pest insects on dryland and irrigated cotton farms. Insectivorous bats selectively source moths and eat mostly pest insects on dryland and irrigated cotton farms
    Article Snippet: .. PCR amplicons were generated using the primers shown in Table , with AmpliTaq Gold® 360 Master Mix (Life Technologies, #4398881) for the primary PCR. .. A secondary 8‐cycle PCR to index the amplicons was performed with TaKaRa Taq™ DNA Polymerase (Clontech, USA #R001B).

    Article Title: Effects of Upconversion Nanoparticles on Polymerase Chain Reaction
    Article Snippet: .. Three commercial PCR master mixes, including the AccuPower PCR PreMix (Bioneer, Daejeon, Korea), AmpliTaq Gold 360 Master Mix (Applied Biosystems, Foster City, CA, USA), and HotStarTaq Plus Master Mix (Qiagen, Valencia, CA, USA), were used for the evaluation of the UCNPs effects on the PCR. .. Top DNA polymerase, used in the AccuPower PCR PreMix, was a modified form of a recombinant DNA polymerase, originally isolated from Thermus thermophilus .

    Article Title: Alterations in the RB1 gene in Pakistani patients with retinoblastoma using direct sequencing analysis
    Article Snippet: .. Genomic DNA was amplified with the thermal cycler ABI 2700 (Applied Biosystems, Foster City, CA) in a total volume of 25 µl containing 25–50 ng of genomic DNA, 1 pmol of forward and reverse primer, 1.5 mM MgCl2 , 1x PCR reaction buffer, 2.5 mM dNTPs, and 1–1.5 U Taq polymerase (AmpliTaq Gold 360 Master Mix; Applied Biosystems, Foster City, CA). .. PCR reactions were performed at initial denaturation at 94 °C for 3 min; ten cycles of 94 °C (45 s), annealing at 67–57 °C with descending one degree in each cycle (45 s), and extension at 72 °C (1 min).

    Article Title: Paratransgenesis to control malaria vectors: a semi-field pilot study
    Article Snippet: .. 50 ng of gDNA was used for PCR amplification in 25 μl reaction volume, containing 2 μM AmpliTaq Gold 360 Master Mix (Applied Biosystem, Foster City, California, USA) and 5 μM each of the oligonucleotide primers. .. All amplifications were performed in a T100 Thermal Cycler (BIO-RAD, CA, USA) with an initial step at 94 °C for 3', followed by 35 cycles: 94 °C for 45 s, 55 °C for 1 min, and 72 °C for 1.5 min, and a final step at 72 °C for 10 min. Metagenomic libraries were prepared using the Nextera XT protocol (Illumina, San Diego, California, USA).

    Article Title: A novel perspective on MOL-PCR optimization and MAGPIX analysis of in-house multiplex foodborne pathogens detection assay
    Article Snippet: .. Several master mixes were tested in the optimization experiments, namely AccuStart II PCR ToughMix (QuantaBio, Massachusetts, USA), AmpliTaq Gold 360 Master Mix (Thermo Fisher Scientific, Massachusetts, USA), OneTaq Hot Start 2X Master Mix with GC Buffer (New England BioLabs, Massachusetts, USA), Platinum Hot Start PCR Master Mix (Invitrogen, California, USA), and HotStarTaq DNA Polymerase (Qiagen, Germany). .. Each singleplex PCR reaction and thermal cycling protocol was run according to the relevant instructions from the manufacturer.

    Generated:

    Article Title: Insectivorous bats selectively source moths and eat mostly pest insects on dryland and irrigated cotton farms. Insectivorous bats selectively source moths and eat mostly pest insects on dryland and irrigated cotton farms
    Article Snippet: .. PCR amplicons were generated using the primers shown in Table , with AmpliTaq Gold® 360 Master Mix (Life Technologies, #4398881) for the primary PCR. .. A secondary 8‐cycle PCR to index the amplicons was performed with TaKaRa Taq™ DNA Polymerase (Clontech, USA #R001B).

    Amplification:

    Article Title: Alterations in the RB1 gene in Pakistani patients with retinoblastoma using direct sequencing analysis
    Article Snippet: .. Genomic DNA was amplified with the thermal cycler ABI 2700 (Applied Biosystems, Foster City, CA) in a total volume of 25 µl containing 25–50 ng of genomic DNA, 1 pmol of forward and reverse primer, 1.5 mM MgCl2 , 1x PCR reaction buffer, 2.5 mM dNTPs, and 1–1.5 U Taq polymerase (AmpliTaq Gold 360 Master Mix; Applied Biosystems, Foster City, CA). .. PCR reactions were performed at initial denaturation at 94 °C for 3 min; ten cycles of 94 °C (45 s), annealing at 67–57 °C with descending one degree in each cycle (45 s), and extension at 72 °C (1 min).

    Article Title: A mild form of Mucopolysaccharidosis IIIB diagnosed with targeted next-generation sequencing of linked genomic regions
    Article Snippet: .. The GC-rich first exon of NAGLU was amplified using AmpliTaq Gold 360 Master Mix (Applied Biosystems). ..

    Article Title: Paratransgenesis to control malaria vectors: a semi-field pilot study
    Article Snippet: .. 50 ng of gDNA was used for PCR amplification in 25 μl reaction volume, containing 2 μM AmpliTaq Gold 360 Master Mix (Applied Biosystem, Foster City, California, USA) and 5 μM each of the oligonucleotide primers. .. All amplifications were performed in a T100 Thermal Cycler (BIO-RAD, CA, USA) with an initial step at 94 °C for 3', followed by 35 cycles: 94 °C for 45 s, 55 °C for 1 min, and 72 °C for 1.5 min, and a final step at 72 °C for 10 min. Metagenomic libraries were prepared using the Nextera XT protocol (Illumina, San Diego, California, USA).

    other:

    Article Title: A novel perspective on MOL-PCR optimization and MAGPIX analysis of in-house multiplex foodborne pathogens detection assay
    Article Snippet: In order to find a balance, the performance of six different master mixes was tested (Fig. ), including the previously utilized HotStarTaq DNA Polymerase – and the upgraded AmpliTaq Gold 360 Master Mix , .

    Hot Start PCR:

    Article Title: A novel perspective on MOL-PCR optimization and MAGPIX analysis of in-house multiplex foodborne pathogens detection assay
    Article Snippet: .. Several master mixes were tested in the optimization experiments, namely AccuStart II PCR ToughMix (QuantaBio, Massachusetts, USA), AmpliTaq Gold 360 Master Mix (Thermo Fisher Scientific, Massachusetts, USA), OneTaq Hot Start 2X Master Mix with GC Buffer (New England BioLabs, Massachusetts, USA), Platinum Hot Start PCR Master Mix (Invitrogen, California, USA), and HotStarTaq DNA Polymerase (Qiagen, Germany). .. Each singleplex PCR reaction and thermal cycling protocol was run according to the relevant instructions from the manufacturer.

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  • 99
    Thermo Fisher amplitaq gold 360 master mix
    Impact evaluation of Master Mix used in singleplex PCR. In graph: Elizyme = 2X EliZyme HS Robust MIX (Elisabeth Pharmacon, Czech Republic); Accustart II = AccuStart II PCR ToughMix (QuantaBio, Massachusetts, USA); <t>Amplitaq</t> = AmpliTaq <t>Gold</t> 360 Master Mix (Thermo Fisher Scientific, Massachusetts, USA); OneTaq = OneTaq Hot Start 2X Master Mix with GC Buffer (New England BioLabs, Massachusetts, USA); Platinum = Platinum Hot Start PCR 2X Master Mix (Invitrogen, California, USA); HotStarTaq = HotStarTaq DNA Polymerase (Qiagen, Germany). YE = Y . enterocolitica; TG = T . gondii ; plus sign in legend = positive sample; minus sign = NTC.
    Amplitaq Gold 360 Master Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 355 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/amplitaq gold 360 master mix/product/Thermo Fisher
    Average 99 stars, based on 355 article reviews
    Price from $9.99 to $1999.99
    amplitaq gold 360 master mix - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

    Image Search Results


    Impact evaluation of Master Mix used in singleplex PCR. In graph: Elizyme = 2X EliZyme HS Robust MIX (Elisabeth Pharmacon, Czech Republic); Accustart II = AccuStart II PCR ToughMix (QuantaBio, Massachusetts, USA); Amplitaq = AmpliTaq Gold 360 Master Mix (Thermo Fisher Scientific, Massachusetts, USA); OneTaq = OneTaq Hot Start 2X Master Mix with GC Buffer (New England BioLabs, Massachusetts, USA); Platinum = Platinum Hot Start PCR 2X Master Mix (Invitrogen, California, USA); HotStarTaq = HotStarTaq DNA Polymerase (Qiagen, Germany). YE = Y . enterocolitica; TG = T . gondii ; plus sign in legend = positive sample; minus sign = NTC.

    Journal: Scientific Reports

    Article Title: A novel perspective on MOL-PCR optimization and MAGPIX analysis of in-house multiplex foodborne pathogens detection assay

    doi: 10.1038/s41598-019-40035-5

    Figure Lengend Snippet: Impact evaluation of Master Mix used in singleplex PCR. In graph: Elizyme = 2X EliZyme HS Robust MIX (Elisabeth Pharmacon, Czech Republic); Accustart II = AccuStart II PCR ToughMix (QuantaBio, Massachusetts, USA); Amplitaq = AmpliTaq Gold 360 Master Mix (Thermo Fisher Scientific, Massachusetts, USA); OneTaq = OneTaq Hot Start 2X Master Mix with GC Buffer (New England BioLabs, Massachusetts, USA); Platinum = Platinum Hot Start PCR 2X Master Mix (Invitrogen, California, USA); HotStarTaq = HotStarTaq DNA Polymerase (Qiagen, Germany). YE = Y . enterocolitica; TG = T . gondii ; plus sign in legend = positive sample; minus sign = NTC.

    Article Snippet: In order to find a balance, the performance of six different master mixes was tested (Fig. ), including the previously utilized HotStarTaq DNA Polymerase – and the upgraded AmpliTaq Gold 360 Master Mix , .

    Techniques: Polymerase Chain Reaction, Hot Start PCR