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PerkinElmer amplitaq dna polymerase
Comparison of the 16S-rRNA-nested-PCR assay with primer pairs ge3a-ge10 and ge9-ge2 with commercially available kits from three manufacturers: (A) PerkinElmer GeneAmp PCR reagent kit with <t>AmpliTaq</t> <t>DNA</t> polymerase; (B) Amersham Pharmacia Ready-To-Go PCR beads; (C) Qiagen Taq PCR Master Mix kit. Lanes 1 through 5 represent amplifications from a 10-fold dilution series ranging from 5 × 10 5 A. phagocytophilum -infected cells/ml to 50 infected cells/ml, and lane 6 contains DNA from uninfected human blood as a negative control. Phage ϕX174 DNA digested with Hae III was run in the lanes labeled M. The arrows indicate the position of the 546-bp amplified products.
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Images

1) Product Images from "Comparison of PCR Assays for Detection of the Agent of Human Granulocytic Ehrlichiosis, Anaplasma phagocytophilum"

Article Title: Comparison of PCR Assays for Detection of the Agent of Human Granulocytic Ehrlichiosis, Anaplasma phagocytophilum

Journal: Journal of Clinical Microbiology

doi: 10.1128/JCM.41.2.717-722.2003

Comparison of the 16S-rRNA-nested-PCR assay with primer pairs ge3a-ge10 and ge9-ge2 with commercially available kits from three manufacturers: (A) PerkinElmer GeneAmp PCR reagent kit with AmpliTaq DNA polymerase; (B) Amersham Pharmacia Ready-To-Go PCR beads; (C) Qiagen Taq PCR Master Mix kit. Lanes 1 through 5 represent amplifications from a 10-fold dilution series ranging from 5 × 10 5 A. phagocytophilum -infected cells/ml to 50 infected cells/ml, and lane 6 contains DNA from uninfected human blood as a negative control. Phage ϕX174 DNA digested with Hae III was run in the lanes labeled M. The arrows indicate the position of the 546-bp amplified products.
Figure Legend Snippet: Comparison of the 16S-rRNA-nested-PCR assay with primer pairs ge3a-ge10 and ge9-ge2 with commercially available kits from three manufacturers: (A) PerkinElmer GeneAmp PCR reagent kit with AmpliTaq DNA polymerase; (B) Amersham Pharmacia Ready-To-Go PCR beads; (C) Qiagen Taq PCR Master Mix kit. Lanes 1 through 5 represent amplifications from a 10-fold dilution series ranging from 5 × 10 5 A. phagocytophilum -infected cells/ml to 50 infected cells/ml, and lane 6 contains DNA from uninfected human blood as a negative control. Phage ϕX174 DNA digested with Hae III was run in the lanes labeled M. The arrows indicate the position of the 546-bp amplified products.

Techniques Used: Nested PCR, Polymerase Chain Reaction, Infection, Negative Control, Labeling, Amplification

2) Product Images from "Comparison of PCR Assays for Detection of the Agent of Human Granulocytic Ehrlichiosis, Anaplasma phagocytophilum"

Article Title: Comparison of PCR Assays for Detection of the Agent of Human Granulocytic Ehrlichiosis, Anaplasma phagocytophilum

Journal: Journal of Clinical Microbiology

doi: 10.1128/JCM.41.2.717-722.2003

Comparison of the 16S-rRNA-nested-PCR assay with primer pairs ge3a-ge10 and ge9-ge2 with commercially available kits from three manufacturers: (A) PerkinElmer GeneAmp PCR reagent kit with AmpliTaq DNA polymerase; (B) Amersham Pharmacia Ready-To-Go PCR beads; (C) Qiagen Taq PCR Master Mix kit. Lanes 1 through 5 represent amplifications from a 10-fold dilution series ranging from 5 × 10 5 A. phagocytophilum -infected cells/ml to 50 infected cells/ml, and lane 6 contains DNA from uninfected human blood as a negative control. Phage ϕX174 DNA digested with Hae III was run in the lanes labeled M. The arrows indicate the position of the 546-bp amplified products.
Figure Legend Snippet: Comparison of the 16S-rRNA-nested-PCR assay with primer pairs ge3a-ge10 and ge9-ge2 with commercially available kits from three manufacturers: (A) PerkinElmer GeneAmp PCR reagent kit with AmpliTaq DNA polymerase; (B) Amersham Pharmacia Ready-To-Go PCR beads; (C) Qiagen Taq PCR Master Mix kit. Lanes 1 through 5 represent amplifications from a 10-fold dilution series ranging from 5 × 10 5 A. phagocytophilum -infected cells/ml to 50 infected cells/ml, and lane 6 contains DNA from uninfected human blood as a negative control. Phage ϕX174 DNA digested with Hae III was run in the lanes labeled M. The arrows indicate the position of the 546-bp amplified products.

Techniques Used: Nested PCR, Polymerase Chain Reaction, Infection, Negative Control, Labeling, Amplification

3) Product Images from "Comparison of PCR Assays for Detection of the Agent of Human Granulocytic Ehrlichiosis, Anaplasma phagocytophilum"

Article Title: Comparison of PCR Assays for Detection of the Agent of Human Granulocytic Ehrlichiosis, Anaplasma phagocytophilum

Journal: Journal of Clinical Microbiology

doi: 10.1128/JCM.41.2.717-722.2003

Comparison of the 16S-rRNA-nested-PCR assay with primer pairs ge3a-ge10 and ge9-ge2 with commercially available kits from three manufacturers: (A) PerkinElmer GeneAmp PCR reagent kit with AmpliTaq DNA polymerase; (B) Amersham Pharmacia Ready-To-Go PCR beads; (C) Qiagen Taq PCR Master Mix kit. Lanes 1 through 5 represent amplifications from a 10-fold dilution series ranging from 5 × 10 5 A. phagocytophilum -infected cells/ml to 50 infected cells/ml, and lane 6 contains DNA from uninfected human blood as a negative control. Phage ϕX174 DNA digested with Hae III was run in the lanes labeled M. The arrows indicate the position of the 546-bp amplified products.
Figure Legend Snippet: Comparison of the 16S-rRNA-nested-PCR assay with primer pairs ge3a-ge10 and ge9-ge2 with commercially available kits from three manufacturers: (A) PerkinElmer GeneAmp PCR reagent kit with AmpliTaq DNA polymerase; (B) Amersham Pharmacia Ready-To-Go PCR beads; (C) Qiagen Taq PCR Master Mix kit. Lanes 1 through 5 represent amplifications from a 10-fold dilution series ranging from 5 × 10 5 A. phagocytophilum -infected cells/ml to 50 infected cells/ml, and lane 6 contains DNA from uninfected human blood as a negative control. Phage ϕX174 DNA digested with Hae III was run in the lanes labeled M. The arrows indicate the position of the 546-bp amplified products.

Techniques Used: Nested PCR, Polymerase Chain Reaction, Infection, Negative Control, Labeling, Amplification

4) Product Images from "Comparison of PCR Assays for Detection of the Agent of Human Granulocytic Ehrlichiosis, Anaplasma phagocytophilum"

Article Title: Comparison of PCR Assays for Detection of the Agent of Human Granulocytic Ehrlichiosis, Anaplasma phagocytophilum

Journal: Journal of Clinical Microbiology

doi: 10.1128/JCM.41.2.717-722.2003

Comparison of the 16S-rRNA-nested-PCR assay with primer pairs ge3a-ge10 and ge9-ge2 with commercially available kits from three manufacturers: (A) PerkinElmer GeneAmp PCR reagent kit with AmpliTaq DNA polymerase; (B) Amersham Pharmacia Ready-To-Go PCR beads; (C) Qiagen Taq PCR Master Mix kit. Lanes 1 through 5 represent amplifications from a 10-fold dilution series ranging from 5 × 10 5 A. phagocytophilum -infected cells/ml to 50 infected cells/ml, and lane 6 contains DNA from uninfected human blood as a negative control. Phage ϕX174 DNA digested with Hae III was run in the lanes labeled M. The arrows indicate the position of the 546-bp amplified products.
Figure Legend Snippet: Comparison of the 16S-rRNA-nested-PCR assay with primer pairs ge3a-ge10 and ge9-ge2 with commercially available kits from three manufacturers: (A) PerkinElmer GeneAmp PCR reagent kit with AmpliTaq DNA polymerase; (B) Amersham Pharmacia Ready-To-Go PCR beads; (C) Qiagen Taq PCR Master Mix kit. Lanes 1 through 5 represent amplifications from a 10-fold dilution series ranging from 5 × 10 5 A. phagocytophilum -infected cells/ml to 50 infected cells/ml, and lane 6 contains DNA from uninfected human blood as a negative control. Phage ϕX174 DNA digested with Hae III was run in the lanes labeled M. The arrows indicate the position of the 546-bp amplified products.

Techniques Used: Nested PCR, Polymerase Chain Reaction, Infection, Negative Control, Labeling, Amplification

Related Articles

Clone Assay:

Article Title: Genome-Wide Characterization of Tetrahymena thermophila Chromosome Breakage Sites. I. Cloning and Identification of Functional Sites
Article Snippet: A total of 3.5 μl of the second-cycle eluate was used as template in a 30-μl PCR reaction that also contained 3 μl of 10× PCR buffer (500 m m KCl, 100 m m Tris-HCl pH 8.3, 15 m m MgCl2 , 0.01% gelatin), 3 μl of 10 m m MgCl2 , 7.2 μl of dNTPs (dissolved in water at 1.25 m m each), 2.5 μl of 20 μ m CP1 primer, and 0.15 μl of AmpliTaq DNA polymerase (Perkin-Elmer, Norwalk, CT) at 5 units/μl. .. PCR reaction products were cloned into the plasmid pCR2.1-TOPO (Invitrogen, San Diego) and transformed into chemically competent TOP10 cells according to the supplier's instructions.

Article Title: Immunolocalization of Sprouty-1 and Sprouty-2 in Developing Rat Lung
Article Snippet: Paragraph title: PCR Cloning of Rat Sprouty Homologs ... The products were amplified using AmpliTaq DNA polymerase (Perkin Elmer Cetus).

Article Title: The Nucleotide Sequence of Shiga Toxin (Stx) 2e-Encoding Phage ?P27 Is Not Related to Other Stx Phage Genomes, but the Modular Genetic Structure Is Conserved
Article Snippet: Random Eco RI, Bam HI, Sal I, Sph I, Kpn I, and Pst I fragments of φP27 were cloned in pK18, and initial sequence information of these clones was obtained with universal and reverse primers for pUC/M13 vectors. .. The amplification reaction was performed in a total volume of 50 μl containing 5 μl of bacterial suspension, 30 pmol of each primer, 5 μl of 10-fold Taq DNA polymerase buffer, 3 μl of MgCl2 stock solution, and 2 U of Amplitaq DNA polymerase (Perkin-Elmer/Applied Biosystems).

Amplification:

Article Title: Interferon (IFN)-? Activation of Human Blood Mononuclear Cells In Vitro and In Vivo for Nitric Oxide Synthase (NOS) Type 2 mRNA and Protein Expression: Possible Relationship of Induced NOS2 to the Anti-Hepatitis C Effects of IFN-? In Vivo
Article Snippet: .. The final product was then amplified with 2.5 U of AmpliTaq® DNA polymerase (Perkin Elmer) and 0.15 μM of sense and antisense primers in PCR buffer containing 100 mM Tris-HCl, 50 mM KCl, 25 mM MgCl2 , and 10 μM deoxyribonucleotide triphosphate. .. Mixtures were overlaid with mineral oil and amplified for 40 cycles.

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Article Snippet: .. For PCR amplification, one-tenth of the RT reaction was combined with 30 pmol of RT primer and 30 pmol of T7 primer (see Fig. A) in 50 µl of the supplied PCR buffer, which also contained 1.5 mM MgCl2 , 0.2 mM dNTP mix and 2.5 U of Amplitaq DNA polymerase (Perkin-Elmer). ..

Article Title: Genome-Wide Characterization of Tetrahymena thermophila Chromosome Breakage Sites. I. Cloning and Identification of Functional Sites
Article Snippet: The eluted single-stranded DNA was next PCR amplified. .. A total of 3.5 μl of the second-cycle eluate was used as template in a 30-μl PCR reaction that also contained 3 μl of 10× PCR buffer (500 m m KCl, 100 m m Tris-HCl pH 8.3, 15 m m MgCl2 , 0.01% gelatin), 3 μl of 10 m m MgCl2 , 7.2 μl of dNTPs (dissolved in water at 1.25 m m each), 2.5 μl of 20 μ m CP1 primer, and 0.15 μl of AmpliTaq DNA polymerase (Perkin-Elmer, Norwalk, CT) at 5 units/μl.

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Article Title: Molecular Characteristics and Epidemiological Significance of Shiga Toxin-Producing Escherichia coli O26 Strains
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Article Title: Human Immunodeficiency Virus Type 1 Replication in the Absence of Integrase-Mediated DNA Recombination: Definition of Permissive and Nonpermissive T-Cell Lines
Article Snippet: Following heat inactivation (65°C for 10 min), DNA was recovered by precipitation with ethanol and was resuspended in 10 μl of H2 O, and 2 μl was amplified using nested PCR. .. The first round (50 μl) contained 0.5 μM (each) primers AE459 ( ) and AE452 ( ) and 2 U of AmpliTaq DNA polymerase (Perkin-Elmer, Foster City, Calif.) in buffer recommended by the manufacturer.

Article Title: The Nucleotide Sequence of Shiga Toxin (Stx) 2e-Encoding Phage ?P27 Is Not Related to Other Stx Phage Genomes, but the Modular Genetic Structure Is Conserved
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Article Title: Differentiation of Cryptosporidium parvum Isolates by a Simplified Randomly Amplified Polymorphic DNA Technique
Article Snippet: A 25-μl PCR mixture, containing 1× PCR Buffer II (Perkin-Elmer Cetus Corp., Norwalk, Conn.), 4.0 mM MgCl2 solution (Perkin-Elmer), a 200 μM concentration of each of the four deoxynucleoside triphosphates (Perkin-Elmer), a 1.0 μM concentration of a 10-nt-long primer with 60% GC content, 2.5 U of AmpliTaq DNA polymerase (Perkin-Elmer), and approximately 1.0 ng of DNA template, was overlaid with 25 μl of mineral oil (Sigma) to prevent evaporation. .. A 20-μl portion of the amplified products was separated on a 1.8% agarose gel and visualized on a UV transilluminator after being stained with 0.5 μg of ethidium bromide per ml.

Article Title: Comparison of PCR Assays for Detection of the Agent of Human Granulocytic Ehrlichiosis, Anaplasma phagocytophilum
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Article Title: Comparison of PCR Assays for Detection of the Agent of Human Granulocytic Ehrlichiosis, Anaplasma phagocytophilum
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Synthesized:

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Article Title: Fusion Activity of Transmembrane and Cytoplasmic Domain Chimeras of the Influenza Virus Glycoprotein Hemagglutinin
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Electrophoresis:

Article Title: An Essential Role of S-Adenosyl-l-Methionine:l-Methionine S-Methyltransferase in Selenium Volatilization by Plants. Methylation of Selenomethionine to Selenium-Methyl-l-Selenium- Methionine, the Precursor of Volatile Selenium 1
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Incubation:

Article Title: RNA structure-dependent uncoupling of substrate recognition and cleavage by Escherichia coli ribonuclease III
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Article Title: Differentiation of Cryptosporidium parvum Isolates by a Simplified Randomly Amplified Polymorphic DNA Technique
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Cell Fractionation:

Article Title: Human Immunodeficiency Virus Type 1 Replication in the Absence of Integrase-Mediated DNA Recombination: Definition of Permissive and Nonpermissive T-Cell Lines
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Transformation Assay:

Article Title: Genome-Wide Characterization of Tetrahymena thermophila Chromosome Breakage Sites. I. Cloning and Identification of Functional Sites
Article Snippet: A total of 3.5 μl of the second-cycle eluate was used as template in a 30-μl PCR reaction that also contained 3 μl of 10× PCR buffer (500 m m KCl, 100 m m Tris-HCl pH 8.3, 15 m m MgCl2 , 0.01% gelatin), 3 μl of 10 m m MgCl2 , 7.2 μl of dNTPs (dissolved in water at 1.25 m m each), 2.5 μl of 20 μ m CP1 primer, and 0.15 μl of AmpliTaq DNA polymerase (Perkin-Elmer, Norwalk, CT) at 5 units/μl. .. PCR reaction products were cloned into the plasmid pCR2.1-TOPO (Invitrogen, San Diego) and transformed into chemically competent TOP10 cells according to the supplier's instructions.

Derivative Assay:

Article Title: Immunolocalization of Sprouty-1 and Sprouty-2 in Developing Rat Lung
Article Snippet: Rat Sprouty-1 and Sprouty-2 cDNAs were obtained by RT-PCR using RNA obtained from adult rat lungs and PCR primers derived from published mouse cDNA sequences [ ]. .. The products were amplified using AmpliTaq DNA polymerase (Perkin Elmer Cetus).

Hybridization:

Article Title: An Essential Role of S-Adenosyl-l-Methionine:l-Methionine S-Methyltransferase in Selenium Volatilization by Plants. Methylation of Selenomethionine to Selenium-Methyl-l-Selenium- Methionine, the Precursor of Volatile Selenium 1
Article Snippet: RNA electrophoresis, northern blotting, hybridization, and washing were performed as described by . .. RT-PCR was performed using RT Superscript II (Life Technologies/Gibco-BRL) and Turbo Pfu DNA polymerase (Stratagene) or Amplitaq DNA polymerase (Perkin-Elmer Applied Biosystems) as described in the user instructions for Superscript II RT.

Southern Blot:

Article Title: Human Immunodeficiency Virus Type 1 Replication in the Absence of Integrase-Mediated DNA Recombination: Definition of Permissive and Nonpermissive T-Cell Lines
Article Snippet: Paragraph title: Cell fractionation, Southern blotting, and PCR. ... The first round (50 μl) contained 0.5 μM (each) primers AE459 ( ) and AE452 ( ) and 2 U of AmpliTaq DNA polymerase (Perkin-Elmer, Foster City, Calif.) in buffer recommended by the manufacturer.

Northern Blot:

Article Title: An Essential Role of S-Adenosyl-l-Methionine:l-Methionine S-Methyltransferase in Selenium Volatilization by Plants. Methylation of Selenomethionine to Selenium-Methyl-l-Selenium- Methionine, the Precursor of Volatile Selenium 1
Article Snippet: RNA electrophoresis, northern blotting, hybridization, and washing were performed as described by . .. RT-PCR was performed using RT Superscript II (Life Technologies/Gibco-BRL) and Turbo Pfu DNA polymerase (Stratagene) or Amplitaq DNA polymerase (Perkin-Elmer Applied Biosystems) as described in the user instructions for Superscript II RT.

Infection:

Article Title: Comparison of PCR Assays for Detection of the Agent of Human Granulocytic Ehrlichiosis, Anaplasma phagocytophilum
Article Snippet: Tenfold serial dilutions of HL-60 cells infected with the USG3 strain of A. phagocytophilum were made in a background of uninfected human blood. .. The initial limit of detection testing used reagents from the GeneAmp PCR kit with AmpliTaq DNA polymerase (PerkinElmer, Foster City, Calif.).

Generated:

Article Title: The Nucleotide Sequence of Shiga Toxin (Stx) 2e-Encoding Phage ?P27 Is Not Related to Other Stx Phage Genomes, but the Modular Genetic Structure Is Conserved
Article Snippet: The amplification reaction was performed in a total volume of 50 μl containing 5 μl of bacterial suspension, 30 pmol of each primer, 5 μl of 10-fold Taq DNA polymerase buffer, 3 μl of MgCl2 stock solution, and 2 U of Amplitaq DNA polymerase (Perkin-Elmer/Applied Biosystems). .. An int probe was generated by PCR with primers 2771-12 (5′-GAAAACCATCTGGGCAC-3′) and 2771-21 (5′-GCACCACATCAAGGTTC-3′).

Polymerase Chain Reaction:

Article Title: Interferon (IFN)-? Activation of Human Blood Mononuclear Cells In Vitro and In Vivo for Nitric Oxide Synthase (NOS) Type 2 mRNA and Protein Expression: Possible Relationship of Induced NOS2 to the Anti-Hepatitis C Effects of IFN-? In Vivo
Article Snippet: .. The final product was then amplified with 2.5 U of AmpliTaq® DNA polymerase (Perkin Elmer) and 0.15 μM of sense and antisense primers in PCR buffer containing 100 mM Tris-HCl, 50 mM KCl, 25 mM MgCl2 , and 10 μM deoxyribonucleotide triphosphate. .. Mixtures were overlaid with mineral oil and amplified for 40 cycles.

Article Title: RNA structure-dependent uncoupling of substrate recognition and cleavage by Escherichia coli ribonuclease III
Article Snippet: .. For PCR amplification, one-tenth of the RT reaction was combined with 30 pmol of RT primer and 30 pmol of T7 primer (see Fig. A) in 50 µl of the supplied PCR buffer, which also contained 1.5 mM MgCl2 , 0.2 mM dNTP mix and 2.5 U of Amplitaq DNA polymerase (Perkin-Elmer). ..

Article Title: Genome-Wide Characterization of Tetrahymena thermophila Chromosome Breakage Sites. I. Cloning and Identification of Functional Sites
Article Snippet: .. A total of 3.5 μl of the second-cycle eluate was used as template in a 30-μl PCR reaction that also contained 3 μl of 10× PCR buffer (500 m m KCl, 100 m m Tris-HCl pH 8.3, 15 m m MgCl2 , 0.01% gelatin), 3 μl of 10 m m MgCl2 , 7.2 μl of dNTPs (dissolved in water at 1.25 m m each), 2.5 μl of 20 μ m CP1 primer, and 0.15 μl of AmpliTaq DNA polymerase (Perkin-Elmer, Norwalk, CT) at 5 units/μl. .. Temperature cycling conditions were: 5 min at 90°, followed by 30 cycles of 30 sec at 90°, 30 sec at 58°, and 3 min at 68°, followed by a terminal extension period of 20 min at 68°.

Article Title: Fusion Activity of Transmembrane and Cytoplasmic Domain Chimeras of the Influenza Virus Glycoprotein Hemagglutinin
Article Snippet: AmpliTaq DNA polymerase was obtained from Perkin-Elmer. .. The ExSite PCR-based site-directed mutagenesis kit was purchased from Stratagene; the Sculptor in vitro mutagenesis system was purchased from Amersham.

Article Title: Immunolocalization of Sprouty-1 and Sprouty-2 in Developing Rat Lung
Article Snippet: Paragraph title: PCR Cloning of Rat Sprouty Homologs ... The products were amplified using AmpliTaq DNA polymerase (Perkin Elmer Cetus).

Article Title: Molecular Characteristics and Epidemiological Significance of Shiga Toxin-Producing Escherichia coli O26 Strains
Article Snippet: .. PCRs for detecting STEC-specific sequences were performed in the GeneAmp PCR System 9600 (Perkin-Elmer Applied Biosystems) in a volume of 50 μl containing 5 μl of bacterial suspension (104 bacteria), 200 μM deoxynucleoside triphosphates (dATP, dCTP, dGTP, and dTTP), 30 pmol of each primer, 5 μl of 10-fold-concentrated polymerase synthesis buffer, 1.5 mM MgCl2 , and 2.0 U of AmpliTaq DNA polymerase (Perkin-Elmer Applied Biosystems). .. To detect stx genes, primer pairs KS7-KS8 ( stx1 B ) and GK3-GK4 ( stx2 B , stx2c B ) were used as described previously ( , ). stx2 and stx2c were differentiated by restriction analysis of GK3-GK4 amplification products using Hae III or Fok I ( ). eae and pas genes were detected using primer pairs SK1-SK2 and ANK49-ANK50, respectively, as described earlier ( , ).

Article Title: Human Immunodeficiency Virus Type 1 Replication in the Absence of Integrase-Mediated DNA Recombination: Definition of Permissive and Nonpermissive T-Cell Lines
Article Snippet: Paragraph title: Cell fractionation, Southern blotting, and PCR. ... The first round (50 μl) contained 0.5 μM (each) primers AE459 ( ) and AE452 ( ) and 2 U of AmpliTaq DNA polymerase (Perkin-Elmer, Foster City, Calif.) in buffer recommended by the manufacturer.

Article Title: The Nucleotide Sequence of Shiga Toxin (Stx) 2e-Encoding Phage ?P27 Is Not Related to Other Stx Phage Genomes, but the Modular Genetic Structure Is Conserved
Article Snippet: PCR was conducted with the GeneAmp 9600 PCR System (Perkin-Elmer/Applied Biosystems). .. The amplification reaction was performed in a total volume of 50 μl containing 5 μl of bacterial suspension, 30 pmol of each primer, 5 μl of 10-fold Taq DNA polymerase buffer, 3 μl of MgCl2 stock solution, and 2 U of Amplitaq DNA polymerase (Perkin-Elmer/Applied Biosystems).

Article Title: Differentiation of Cryptosporidium parvum Isolates by a Simplified Randomly Amplified Polymorphic DNA Technique
Article Snippet: .. A 25-μl PCR mixture, containing 1× PCR Buffer II (Perkin-Elmer Cetus Corp., Norwalk, Conn.), 4.0 mM MgCl2 solution (Perkin-Elmer), a 200 μM concentration of each of the four deoxynucleoside triphosphates (Perkin-Elmer), a 1.0 μM concentration of a 10-nt-long primer with 60% GC content, 2.5 U of AmpliTaq DNA polymerase (Perkin-Elmer), and approximately 1.0 ng of DNA template, was overlaid with 25 μl of mineral oil (Sigma) to prevent evaporation. ..

Article Title: Comparison of PCR Assays for Detection of the Agent of Human Granulocytic Ehrlichiosis, Anaplasma phagocytophilum
Article Snippet: .. All assays tested were screened for specificity with template DNA from R. rickettsii , B. henselae , and E. chaffeensis by using the GeneAmp PCR kit with AmpliTaq DNA polymerase (PerkinElmer). ..

Article Title: Comparison of PCR Assays for Detection of the Agent of Human Granulocytic Ehrlichiosis, Anaplasma phagocytophilum
Article Snippet: .. The initial limit of detection testing used reagents from the GeneAmp PCR kit with AmpliTaq DNA polymerase (PerkinElmer, Foster City, Calif.). ..

Article Title: Comparison of PCR Assays for Detection of the Agent of Human Granulocytic Ehrlichiosis, Anaplasma phagocytophilum
Article Snippet: .. Three commercially available PCR amplification kits were used for this comparison, although each of the kits used AmpliTaq DNA polymerase and the same magnesium concentration (1.5 mM). .. The nested-16S assay showed little difference when tested with the three kits (Fig. ), and each clearly detected 0.25 infected cell.

Article Title: Comparison of PCR Assays for Detection of the Agent of Human Granulocytic Ehrlichiosis, Anaplasma phagocytophilum
Article Snippet: .. These extractions were used with the three optimal assays to compare the PCR amplification kits from three different manufacturers: the GeneAmp PCR kit with AmpliTaq DNA polymerase (PerkinElmer), Ready-To-Go PCR beads (Amersham Pharmacia Biotech, Piscataway, N.J.), and the Taq PCR Master Mix kit (Qiagen). ..

Sequencing:

Article Title: Fusion Activity of Transmembrane and Cytoplasmic Domain Chimeras of the Influenza Virus Glycoprotein Hemagglutinin
Article Snippet: AmpliTaq DNA polymerase was obtained from Perkin-Elmer. .. Oligonucleotide primers used for sequencing and PCR were synthesized by Pharmacia Biotech and Gibco BRL.

Article Title: The Nucleotide Sequence of Shiga Toxin (Stx) 2e-Encoding Phage ?P27 Is Not Related to Other Stx Phage Genomes, but the Modular Genetic Structure Is Conserved
Article Snippet: Random Eco RI, Bam HI, Sal I, Sph I, Kpn I, and Pst I fragments of φP27 were cloned in pK18, and initial sequence information of these clones was obtained with universal and reverse primers for pUC/M13 vectors. .. The amplification reaction was performed in a total volume of 50 μl containing 5 μl of bacterial suspension, 30 pmol of each primer, 5 μl of 10-fold Taq DNA polymerase buffer, 3 μl of MgCl2 stock solution, and 2 U of Amplitaq DNA polymerase (Perkin-Elmer/Applied Biosystems).

DNA Extraction:

Article Title: Differentiation of Cryptosporidium parvum Isolates by a Simplified Randomly Amplified Polymorphic DNA Technique
Article Snippet: The DNA concentration was estimated with a DNA DipStick (Invitrogen, Carlsbad, Calif.) as described in the manufacturer’s instructions and was referred to as the number of purified oocysts used for IMS and DNA extraction. .. A 25-μl PCR mixture, containing 1× PCR Buffer II (Perkin-Elmer Cetus Corp., Norwalk, Conn.), 4.0 mM MgCl2 solution (Perkin-Elmer), a 200 μM concentration of each of the four deoxynucleoside triphosphates (Perkin-Elmer), a 1.0 μM concentration of a 10-nt-long primer with 60% GC content, 2.5 U of AmpliTaq DNA polymerase (Perkin-Elmer), and approximately 1.0 ng of DNA template, was overlaid with 25 μl of mineral oil (Sigma) to prevent evaporation.

Nucleic Acid Electrophoresis:

Article Title: RNA structure-dependent uncoupling of substrate recognition and cleavage by Escherichia coli ribonuclease III
Article Snippet: An aliquot was analyzed by gel electrophoresis to assess extent of cleavage (see above). .. For PCR amplification, one-tenth of the RT reaction was combined with 30 pmol of RT primer and 30 pmol of T7 primer (see Fig. A) in 50 µl of the supplied PCR buffer, which also contained 1.5 mM MgCl2 , 0.2 mM dNTP mix and 2.5 U of Amplitaq DNA polymerase (Perkin-Elmer).

Magnetic Beads:

Article Title: Differentiation of Cryptosporidium parvum Isolates by a Simplified Randomly Amplified Polymorphic DNA Technique
Article Snippet: Briefly, a more-concentrated ERS1-2 sample or a purified oocyst suspension containing ∼5 × 105 oocysts was incubated with IgG-coated magnetic beads, separated from contaminating materials, and subjected to in vitro excystation; DNA was released from sporozoites or partially excysted oocysts by incubation at 95°C for 10 min, as in the IC-PCR. .. A 25-μl PCR mixture, containing 1× PCR Buffer II (Perkin-Elmer Cetus Corp., Norwalk, Conn.), 4.0 mM MgCl2 solution (Perkin-Elmer), a 200 μM concentration of each of the four deoxynucleoside triphosphates (Perkin-Elmer), a 1.0 μM concentration of a 10-nt-long primer with 60% GC content, 2.5 U of AmpliTaq DNA polymerase (Perkin-Elmer), and approximately 1.0 ng of DNA template, was overlaid with 25 μl of mineral oil (Sigma) to prevent evaporation.

Mutagenesis:

Article Title: Vibrio fischeri Flagellin A Is Essential for Normal Motility and for Symbiotic Competence during Initial Squid Light Organ Colonization
Article Snippet: Growth of mutant strains in cultures was determined by using SWT media, and luminescence was determined as described previously ( ). .. AmpliTaq DNA polymerase was obtained from Perkin-Elmer (Branchburg, N.J.).

Article Title: Fusion Activity of Transmembrane and Cytoplasmic Domain Chimeras of the Influenza Virus Glycoprotein Hemagglutinin
Article Snippet: AmpliTaq DNA polymerase was obtained from Perkin-Elmer. .. The ExSite PCR-based site-directed mutagenesis kit was purchased from Stratagene; the Sculptor in vitro mutagenesis system was purchased from Amersham.

Isolation:

Article Title: Interferon (IFN)-? Activation of Human Blood Mononuclear Cells In Vitro and In Vivo for Nitric Oxide Synthase (NOS) Type 2 mRNA and Protein Expression: Possible Relationship of Induced NOS2 to the Anti-Hepatitis C Effects of IFN-? In Vivo
Article Snippet: Total RNA was isolated by the method of Chomczynski and Sacchi , resuspended in diethyl pyrocarbonate–treated water, and reprecipitated overnight with cold isopropanol at −20°C. .. The final product was then amplified with 2.5 U of AmpliTaq® DNA polymerase (Perkin Elmer) and 0.15 μM of sense and antisense primers in PCR buffer containing 100 mM Tris-HCl, 50 mM KCl, 25 mM MgCl2 , and 10 μM deoxyribonucleotide triphosphate.

Article Title: The Nucleotide Sequence of Shiga Toxin (Stx) 2e-Encoding Phage ?P27 Is Not Related to Other Stx Phage Genomes, but the Modular Genetic Structure Is Conserved
Article Snippet: The amplification reaction was performed in a total volume of 50 μl containing 5 μl of bacterial suspension, 30 pmol of each primer, 5 μl of 10-fold Taq DNA polymerase buffer, 3 μl of MgCl2 stock solution, and 2 U of Amplitaq DNA polymerase (Perkin-Elmer/Applied Biosystems). .. Whole bacterial DNA was isolated as described by Heuvelink et al. ( ).

Article Title: An Essential Role of S-Adenosyl-l-Methionine:l-Methionine S-Methyltransferase in Selenium Volatilization by Plants. Methylation of Selenomethionine to Selenium-Methyl-l-Selenium- Methionine, the Precursor of Volatile Selenium 1
Article Snippet: Genomic DNA was isolated from Arabidopsis using the cetyl-trimethyl-ammonium bromide method ( ). .. RT-PCR was performed using RT Superscript II (Life Technologies/Gibco-BRL) and Turbo Pfu DNA polymerase (Stratagene) or Amplitaq DNA polymerase (Perkin-Elmer Applied Biosystems) as described in the user instructions for Superscript II RT.

Size-exclusion Chromatography:

Article Title: Genome-Wide Characterization of Tetrahymena thermophila Chromosome Breakage Sites. I. Cloning and Identification of Functional Sites
Article Snippet: A total of 3.5 μl of the second-cycle eluate was used as template in a 30-μl PCR reaction that also contained 3 μl of 10× PCR buffer (500 m m KCl, 100 m m Tris-HCl pH 8.3, 15 m m MgCl2 , 0.01% gelatin), 3 μl of 10 m m MgCl2 , 7.2 μl of dNTPs (dissolved in water at 1.25 m m each), 2.5 μl of 20 μ m CP1 primer, and 0.15 μl of AmpliTaq DNA polymerase (Perkin-Elmer, Norwalk, CT) at 5 units/μl. .. Temperature cycling conditions were: 5 min at 90°, followed by 30 cycles of 30 sec at 90°, 30 sec at 58°, and 3 min at 68°, followed by a terminal extension period of 20 min at 68°.

Labeling:

Article Title: An Essential Role of S-Adenosyl-l-Methionine:l-Methionine S-Methyltransferase in Selenium Volatilization by Plants. Methylation of Selenomethionine to Selenium-Methyl-l-Selenium- Methionine, the Precursor of Volatile Selenium 1
Article Snippet: MMT-DNA probes were labeled with [32 P]dCTP by random priming (Ready to go Kit, Amersham, Buckinghamshire, UK). .. RT-PCR was performed using RT Superscript II (Life Technologies/Gibco-BRL) and Turbo Pfu DNA polymerase (Stratagene) or Amplitaq DNA polymerase (Perkin-Elmer Applied Biosystems) as described in the user instructions for Superscript II RT.

Purification:

Article Title: RNA structure-dependent uncoupling of substrate recognition and cleavage by Escherichia coli ribonuclease III
Article Snippet: For PCR amplification, one-tenth of the RT reaction was combined with 30 pmol of RT primer and 30 pmol of T7 primer (see Fig. A) in 50 µl of the supplied PCR buffer, which also contained 1.5 mM MgCl2 , 0.2 mM dNTP mix and 2.5 U of Amplitaq DNA polymerase (Perkin-Elmer). .. The PCR products were gel purified using a Qiaquick MinElute kit (Qiagen) or a Zymo-Clean kit (Zymo Research, Orange, CA).

Article Title: Differentiation of Cryptosporidium parvum Isolates by a Simplified Randomly Amplified Polymorphic DNA Technique
Article Snippet: The DNA concentration was estimated with a DNA DipStick (Invitrogen, Carlsbad, Calif.) as described in the manufacturer’s instructions and was referred to as the number of purified oocysts used for IMS and DNA extraction. .. A 25-μl PCR mixture, containing 1× PCR Buffer II (Perkin-Elmer Cetus Corp., Norwalk, Conn.), 4.0 mM MgCl2 solution (Perkin-Elmer), a 200 μM concentration of each of the four deoxynucleoside triphosphates (Perkin-Elmer), a 1.0 μM concentration of a 10-nt-long primer with 60% GC content, 2.5 U of AmpliTaq DNA polymerase (Perkin-Elmer), and approximately 1.0 ng of DNA template, was overlaid with 25 μl of mineral oil (Sigma) to prevent evaporation.

Reverse Transcription Polymerase Chain Reaction:

Article Title: Immunolocalization of Sprouty-1 and Sprouty-2 in Developing Rat Lung
Article Snippet: Rat Sprouty-1 and Sprouty-2 cDNAs were obtained by RT-PCR using RNA obtained from adult rat lungs and PCR primers derived from published mouse cDNA sequences [ ]. .. The products were amplified using AmpliTaq DNA polymerase (Perkin Elmer Cetus).

Article Title: An Essential Role of S-Adenosyl-l-Methionine:l-Methionine S-Methyltransferase in Selenium Volatilization by Plants. Methylation of Selenomethionine to Selenium-Methyl-l-Selenium- Methionine, the Precursor of Volatile Selenium 1
Article Snippet: .. RT-PCR was performed using RT Superscript II (Life Technologies/Gibco-BRL) and Turbo Pfu DNA polymerase (Stratagene) or Amplitaq DNA polymerase (Perkin-Elmer Applied Biosystems) as described in the user instructions for Superscript II RT. .. Arabidopsis was grown in liquid cultures at 22°C under permanent light using a modified protocol described by .

Nested PCR:

Article Title: Human Immunodeficiency Virus Type 1 Replication in the Absence of Integrase-Mediated DNA Recombination: Definition of Permissive and Nonpermissive T-Cell Lines
Article Snippet: Following heat inactivation (65°C for 10 min), DNA was recovered by precipitation with ethanol and was resuspended in 10 μl of H2 O, and 2 μl was amplified using nested PCR. .. The first round (50 μl) contained 0.5 μM (each) primers AE459 ( ) and AE452 ( ) and 2 U of AmpliTaq DNA polymerase (Perkin-Elmer, Foster City, Calif.) in buffer recommended by the manufacturer.

DNA-DNA Hybridization:

Article Title: The Nucleotide Sequence of Shiga Toxin (Stx) 2e-Encoding Phage ?P27 Is Not Related to Other Stx Phage Genomes, but the Modular Genetic Structure Is Conserved
Article Snippet: The amplification reaction was performed in a total volume of 50 μl containing 5 μl of bacterial suspension, 30 pmol of each primer, 5 μl of 10-fold Taq DNA polymerase buffer, 3 μl of MgCl2 stock solution, and 2 U of Amplitaq DNA polymerase (Perkin-Elmer/Applied Biosystems). .. The amplification reaction was performed in a total volume of 50 μl containing 5 μl of bacterial suspension, 30 pmol of each primer, 5 μl of 10-fold Taq DNA polymerase buffer, 3 μl of MgCl2 stock solution, and 2 U of Amplitaq DNA polymerase (Perkin-Elmer/Applied Biosystems).

Plasmid Preparation:

Article Title: Genome-Wide Characterization of Tetrahymena thermophila Chromosome Breakage Sites. I. Cloning and Identification of Functional Sites
Article Snippet: A total of 3.5 μl of the second-cycle eluate was used as template in a 30-μl PCR reaction that also contained 3 μl of 10× PCR buffer (500 m m KCl, 100 m m Tris-HCl pH 8.3, 15 m m MgCl2 , 0.01% gelatin), 3 μl of 10 m m MgCl2 , 7.2 μl of dNTPs (dissolved in water at 1.25 m m each), 2.5 μl of 20 μ m CP1 primer, and 0.15 μl of AmpliTaq DNA polymerase (Perkin-Elmer, Norwalk, CT) at 5 units/μl. .. PCR reaction products were cloned into the plasmid pCR2.1-TOPO (Invitrogen, San Diego) and transformed into chemically competent TOP10 cells according to the supplier's instructions.

Article Title: Immunolocalization of Sprouty-1 and Sprouty-2 in Developing Rat Lung
Article Snippet: The products were amplified using AmpliTaq DNA polymerase (Perkin Elmer Cetus). .. The amplified DNA was cloned into the TA vector (Invitrogen) and sequenced on an ABI Prism 377 sequencer at the University of Pittsburgh, School of Medicine Research Support Facilities.

Selection:

Article Title: RNA structure-dependent uncoupling of substrate recognition and cleavage by Escherichia coli ribonuclease III
Article Snippet: Paragraph title: In vitro selection procedure ... For PCR amplification, one-tenth of the RT reaction was combined with 30 pmol of RT primer and 30 pmol of T7 primer (see Fig. A) in 50 µl of the supplied PCR buffer, which also contained 1.5 mM MgCl2 , 0.2 mM dNTP mix and 2.5 U of Amplitaq DNA polymerase (Perkin-Elmer).

Agarose Gel Electrophoresis:

Article Title: Differentiation of Cryptosporidium parvum Isolates by a Simplified Randomly Amplified Polymorphic DNA Technique
Article Snippet: A 25-μl PCR mixture, containing 1× PCR Buffer II (Perkin-Elmer Cetus Corp., Norwalk, Conn.), 4.0 mM MgCl2 solution (Perkin-Elmer), a 200 μM concentration of each of the four deoxynucleoside triphosphates (Perkin-Elmer), a 1.0 μM concentration of a 10-nt-long primer with 60% GC content, 2.5 U of AmpliTaq DNA polymerase (Perkin-Elmer), and approximately 1.0 ng of DNA template, was overlaid with 25 μl of mineral oil (Sigma) to prevent evaporation. .. A 20-μl portion of the amplified products was separated on a 1.8% agarose gel and visualized on a UV transilluminator after being stained with 0.5 μg of ethidium bromide per ml.

In Vitro:

Article Title: RNA structure-dependent uncoupling of substrate recognition and cleavage by Escherichia coli ribonuclease III
Article Snippet: Paragraph title: In vitro selection procedure ... For PCR amplification, one-tenth of the RT reaction was combined with 30 pmol of RT primer and 30 pmol of T7 primer (see Fig. A) in 50 µl of the supplied PCR buffer, which also contained 1.5 mM MgCl2 , 0.2 mM dNTP mix and 2.5 U of Amplitaq DNA polymerase (Perkin-Elmer).

Article Title: Fusion Activity of Transmembrane and Cytoplasmic Domain Chimeras of the Influenza Virus Glycoprotein Hemagglutinin
Article Snippet: AmpliTaq DNA polymerase was obtained from Perkin-Elmer. .. The ExSite PCR-based site-directed mutagenesis kit was purchased from Stratagene; the Sculptor in vitro mutagenesis system was purchased from Amersham.

Article Title: Differentiation of Cryptosporidium parvum Isolates by a Simplified Randomly Amplified Polymorphic DNA Technique
Article Snippet: Briefly, a more-concentrated ERS1-2 sample or a purified oocyst suspension containing ∼5 × 105 oocysts was incubated with IgG-coated magnetic beads, separated from contaminating materials, and subjected to in vitro excystation; DNA was released from sporozoites or partially excysted oocysts by incubation at 95°C for 10 min, as in the IC-PCR. .. A 25-μl PCR mixture, containing 1× PCR Buffer II (Perkin-Elmer Cetus Corp., Norwalk, Conn.), 4.0 mM MgCl2 solution (Perkin-Elmer), a 200 μM concentration of each of the four deoxynucleoside triphosphates (Perkin-Elmer), a 1.0 μM concentration of a 10-nt-long primer with 60% GC content, 2.5 U of AmpliTaq DNA polymerase (Perkin-Elmer), and approximately 1.0 ng of DNA template, was overlaid with 25 μl of mineral oil (Sigma) to prevent evaporation.

Chromosome Walking:

Article Title: The Nucleotide Sequence of Shiga Toxin (Stx) 2e-Encoding Phage ?P27 Is Not Related to Other Stx Phage Genomes, but the Modular Genetic Structure Is Conserved
Article Snippet: Genomic DNA of phage φP27 was used as the template for primer walking. .. The amplification reaction was performed in a total volume of 50 μl containing 5 μl of bacterial suspension, 30 pmol of each primer, 5 μl of 10-fold Taq DNA polymerase buffer, 3 μl of MgCl2 stock solution, and 2 U of Amplitaq DNA polymerase (Perkin-Elmer/Applied Biosystems).

Evaporation:

Article Title: Differentiation of Cryptosporidium parvum Isolates by a Simplified Randomly Amplified Polymorphic DNA Technique
Article Snippet: .. A 25-μl PCR mixture, containing 1× PCR Buffer II (Perkin-Elmer Cetus Corp., Norwalk, Conn.), 4.0 mM MgCl2 solution (Perkin-Elmer), a 200 μM concentration of each of the four deoxynucleoside triphosphates (Perkin-Elmer), a 1.0 μM concentration of a 10-nt-long primer with 60% GC content, 2.5 U of AmpliTaq DNA polymerase (Perkin-Elmer), and approximately 1.0 ng of DNA template, was overlaid with 25 μl of mineral oil (Sigma) to prevent evaporation. ..

Concentration Assay:

Article Title: RNA structure-dependent uncoupling of substrate recognition and cleavage by Escherichia coli ribonuclease III
Article Snippet: Gel-purified RNA (∼15 000 d.p.m., 5.4 pmol) was incubated with RNase III (200 nM concentration) for 40 min at 37°C. .. For PCR amplification, one-tenth of the RT reaction was combined with 30 pmol of RT primer and 30 pmol of T7 primer (see Fig. A) in 50 µl of the supplied PCR buffer, which also contained 1.5 mM MgCl2 , 0.2 mM dNTP mix and 2.5 U of Amplitaq DNA polymerase (Perkin-Elmer).

Article Title: Vibrio fischeri Flagellin A Is Essential for Normal Motility and for Symbiotic Competence during Initial Squid Light Organ Colonization
Article Snippet: Kanamycin was added to growth media at a concentration of 50 μg ml−1 for Escherichia coli strains and at a concentration of 100 μg ml−1 for V. fischeri strains. .. AmpliTaq DNA polymerase was obtained from Perkin-Elmer (Branchburg, N.J.).

Article Title: Differentiation of Cryptosporidium parvum Isolates by a Simplified Randomly Amplified Polymorphic DNA Technique
Article Snippet: .. A 25-μl PCR mixture, containing 1× PCR Buffer II (Perkin-Elmer Cetus Corp., Norwalk, Conn.), 4.0 mM MgCl2 solution (Perkin-Elmer), a 200 μM concentration of each of the four deoxynucleoside triphosphates (Perkin-Elmer), a 1.0 μM concentration of a 10-nt-long primer with 60% GC content, 2.5 U of AmpliTaq DNA polymerase (Perkin-Elmer), and approximately 1.0 ng of DNA template, was overlaid with 25 μl of mineral oil (Sigma) to prevent evaporation. ..

Article Title: Comparison of PCR Assays for Detection of the Agent of Human Granulocytic Ehrlichiosis, Anaplasma phagocytophilum
Article Snippet: .. Three commercially available PCR amplification kits were used for this comparison, although each of the kits used AmpliTaq DNA polymerase and the same magnesium concentration (1.5 mM). .. The nested-16S assay showed little difference when tested with the three kits (Fig. ), and each clearly detected 0.25 infected cell.

CTG Assay:

Article Title: Interferon (IFN)-? Activation of Human Blood Mononuclear Cells In Vitro and In Vivo for Nitric Oxide Synthase (NOS) Type 2 mRNA and Protein Expression: Possible Relationship of Induced NOS2 to the Anti-Hepatitis C Effects of IFN-? In Vivo
Article Snippet: The final product was then amplified with 2.5 U of AmpliTaq® DNA polymerase (Perkin Elmer) and 0.15 μM of sense and antisense primers in PCR buffer containing 100 mM Tris-HCl, 50 mM KCl, 25 mM MgCl2 , and 10 μM deoxyribonucleotide triphosphate. .. As a control, we used the following primers for the glyceraldehyde-3-phosphate dehydrogenase: 5′-CTA CTG GCG CTG CCA AGG CTG T-3′ (sense) and 5′-GCC ATG AGG TCC ACC ACC CTG T-3′ (antisense).

Staining:

Article Title: Differentiation of Cryptosporidium parvum Isolates by a Simplified Randomly Amplified Polymorphic DNA Technique
Article Snippet: A 25-μl PCR mixture, containing 1× PCR Buffer II (Perkin-Elmer Cetus Corp., Norwalk, Conn.), 4.0 mM MgCl2 solution (Perkin-Elmer), a 200 μM concentration of each of the four deoxynucleoside triphosphates (Perkin-Elmer), a 1.0 μM concentration of a 10-nt-long primer with 60% GC content, 2.5 U of AmpliTaq DNA polymerase (Perkin-Elmer), and approximately 1.0 ng of DNA template, was overlaid with 25 μl of mineral oil (Sigma) to prevent evaporation. .. A 20-μl portion of the amplified products was separated on a 1.8% agarose gel and visualized on a UV transilluminator after being stained with 0.5 μg of ethidium bromide per ml.

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  • 94
    PerkinElmer amplitaq gold dna polymerase
    Amplification of eight copies of HPV-16 accomplished in the presence of TE buffer (lanes b, c, and d), 0.04% (final concentration [vol/vol]) NP-40 and 0.04% Tween (lanes e and f), 0.08% Tween 20 (lanes g and h), and 0.08% NP-40 (lanes i and j). The negative control is in lane a. Amplification was done with <t>AmpliTaq</t> Gold <t>DNA</t> polymerase in a model 9600 DNA thermal cycler. A 450-bp band is visible in positive reactions.
    Amplitaq Gold Dna Polymerase, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 94/100, based on 48 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/amplitaq gold dna polymerase/product/PerkinElmer
    Average 94 stars, based on 48 article reviews
    Price from $9.99 to $1999.99
    amplitaq gold dna polymerase - by Bioz Stars, 2020-02
    94/100 stars
      Buy from Supplier

    99
    PerkinElmer amplitaq dna polymerase
    Comparison of the 16S-rRNA-nested-PCR assay with primer pairs ge3a-ge10 and ge9-ge2 with commercially available kits from three manufacturers: (A) PerkinElmer GeneAmp PCR reagent kit with <t>AmpliTaq</t> <t>DNA</t> polymerase; (B) Amersham Pharmacia Ready-To-Go PCR beads; (C) Qiagen Taq PCR Master Mix kit. Lanes 1 through 5 represent amplifications from a 10-fold dilution series ranging from 5 × 10 5 A. phagocytophilum -infected cells/ml to 50 infected cells/ml, and lane 6 contains DNA from uninfected human blood as a negative control. Phage ϕX174 DNA digested with Hae III was run in the lanes labeled M. The arrows indicate the position of the 546-bp amplified products.
    Amplitaq Dna Polymerase, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 99/100, based on 436 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/amplitaq dna polymerase/product/PerkinElmer
    Average 99 stars, based on 436 article reviews
    Price from $9.99 to $1999.99
    amplitaq dna polymerase - by Bioz Stars, 2020-02
    99/100 stars
      Buy from Supplier

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    Amplification of eight copies of HPV-16 accomplished in the presence of TE buffer (lanes b, c, and d), 0.04% (final concentration [vol/vol]) NP-40 and 0.04% Tween (lanes e and f), 0.08% Tween 20 (lanes g and h), and 0.08% NP-40 (lanes i and j). The negative control is in lane a. Amplification was done with AmpliTaq Gold DNA polymerase in a model 9600 DNA thermal cycler. A 450-bp band is visible in positive reactions.

    Journal: Journal of Clinical Microbiology

    Article Title: Effect of Nonionic Detergents on Amplification of Human Papillomavirus DNA with Consensus Primers MY09 and MY11

    doi:

    Figure Lengend Snippet: Amplification of eight copies of HPV-16 accomplished in the presence of TE buffer (lanes b, c, and d), 0.04% (final concentration [vol/vol]) NP-40 and 0.04% Tween (lanes e and f), 0.08% Tween 20 (lanes g and h), and 0.08% NP-40 (lanes i and j). The negative control is in lane a. Amplification was done with AmpliTaq Gold DNA polymerase in a model 9600 DNA thermal cycler. A 450-bp band is visible in positive reactions.

    Article Snippet: Ten-microliter volumes of the detergent preparations described below were added to 90-μl volumes of a PCR master mix containing the usual components ( , ) plus eight copies of HPV-16 (kindly provided by H. zur Hausen), 5 U of AmpliTaq Gold DNA polymerase (Perkin-Elmer, Montréal, Canada), and the L1 consensus HPV primers MY09 and MY11.

    Techniques: Amplification, Concentration Assay, Negative Control

    Comparison of the 16S-rRNA-nested-PCR assay with primer pairs ge3a-ge10 and ge9-ge2 with commercially available kits from three manufacturers: (A) PerkinElmer GeneAmp PCR reagent kit with AmpliTaq DNA polymerase; (B) Amersham Pharmacia Ready-To-Go PCR beads; (C) Qiagen Taq PCR Master Mix kit. Lanes 1 through 5 represent amplifications from a 10-fold dilution series ranging from 5 × 10 5 A. phagocytophilum -infected cells/ml to 50 infected cells/ml, and lane 6 contains DNA from uninfected human blood as a negative control. Phage ϕX174 DNA digested with Hae III was run in the lanes labeled M. The arrows indicate the position of the 546-bp amplified products.

    Journal: Journal of Clinical Microbiology

    Article Title: Comparison of PCR Assays for Detection of the Agent of Human Granulocytic Ehrlichiosis, Anaplasma phagocytophilum

    doi: 10.1128/JCM.41.2.717-722.2003

    Figure Lengend Snippet: Comparison of the 16S-rRNA-nested-PCR assay with primer pairs ge3a-ge10 and ge9-ge2 with commercially available kits from three manufacturers: (A) PerkinElmer GeneAmp PCR reagent kit with AmpliTaq DNA polymerase; (B) Amersham Pharmacia Ready-To-Go PCR beads; (C) Qiagen Taq PCR Master Mix kit. Lanes 1 through 5 represent amplifications from a 10-fold dilution series ranging from 5 × 10 5 A. phagocytophilum -infected cells/ml to 50 infected cells/ml, and lane 6 contains DNA from uninfected human blood as a negative control. Phage ϕX174 DNA digested with Hae III was run in the lanes labeled M. The arrows indicate the position of the 546-bp amplified products.

    Article Snippet: The initial limit of detection testing used reagents from the GeneAmp PCR kit with AmpliTaq DNA polymerase (PerkinElmer, Foster City, Calif.).

    Techniques: Nested PCR, Polymerase Chain Reaction, Infection, Negative Control, Labeling, Amplification