amplification grade dnase i  (Thermo Fisher)


Bioz Verified Symbol Thermo Fisher is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99

    Structured Review

    Thermo Fisher amplification grade dnase i
    Amplification Grade Dnase I, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 402 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/amplification grade dnase i/product/Thermo Fisher
    Average 99 stars, based on 402 article reviews
    Price from $9.99 to $1999.99
    amplification grade dnase i - by Bioz Stars, 2020-03
    99/100 stars

    Images

    Related Articles

    Real-time Polymerase Chain Reaction:

    Article Title: Tissue-type transglutaminase and the effects of cystamine on intracerebral hemorrhage-induced brain edema and neurological deficits
    Article Snippet: Paragraph title: Real-time quantitative polymerase chain reaction ... Total RNA was extracted with Trizol reagent (Gibco BRL Grand Island, NY, U.S.A.), and 1µg RNA was digested with amplification-grade deoxyribonuclease I (Gibco BRL).

    Article Title: Identification of Redox Partners of the Thiol-Disulfide Oxidoreductase SdbA in Streptococcus gordonii
    Article Snippet: Paragraph title: Reverse transcription-PCR and quantitative real-time PCR. ... DNA was removed from RNA (1 μg) samples by digestion with amplification grade DNase I (Life Technologies) for 15 min at room temperature.

    Article Title: Infection drives meningeal engraftment by inflammatory monocytes that impairs CNS immunity
    Article Snippet: Paragraph title: Quantitative PCR. ... Purified RNA was then treated with amplification-grade DNase I (Invitrogen) to remove contaminating DNA and reverse transcribed into cDNA by using an iScript cDNA Synthesis kit (Life Technologies).

    Article Title: Antiviral activity of zinc salts against transmissible gastroenteritis virus in vitro.
    Article Snippet: Paragraph title: Real-time PCR quantification of viral RNA synthesis ... RNA was eluted in 50 ml RNase-free water and treated with Amplification Grade DNase I (Invitrogen) according to the manufacturer's instructions.

    Article Title: LIM and cysteine-rich domains 1 (LMCD1) regulates skeletal muscle hypertrophy, calcium handling, and force
    Article Snippet: Afterward, 1 μg of RNA was treated with Amplification Grade DNase I (Life Technologies) and from that, 500 ng were used for cDNA preparation using the Applied Biosystem Reverse Transcription Kit (Life Technologies). .. Quantitative real-time PCR was performed in a ViiA 7 Real-Time PCR system thermal cycler with SYBR Green PCR Master Mix (both Applied Biosystems).

    Negative Control:

    Article Title: Infection drives meningeal engraftment by inflammatory monocytes that impairs CNS immunity
    Article Snippet: Purified RNA was then treated with amplification-grade DNase I (Invitrogen) to remove contaminating DNA and reverse transcribed into cDNA by using an iScript cDNA Synthesis kit (Life Technologies). .. Real-time PCR was performed using SYBR Green (Applied Biosystems) and cDNA template or water (non-template negative control) at an annealing temperature of 60 degrees with a CFX96 Real-Time PCR machine (Bio-Rad Laboratories).

    Amplification:

    Article Title: Ribosomal RNA depletion or exclusion has negligible effect on the detection of viruses in a pan viral microarray.
    Article Snippet: .. Nucleic acid was quantified using Nanodrop 2000 spectrometer (Agilent Technologies, Cheshire, UK) and diluted to a concentration of 4 g in 32 l of nuclease free water, from which three aliquots of 8 l were subjected to DNase digest using amplification grade DNase I (Life Technologies, Paisley, UK). .. Briefly, 1 l of 10× DNase buffer and 1 l of DNase I enzyme (1 units/l) were added to each 8 l nucleic acid extract and incubated at 37 • C for 30 min. 1 l of 25 mM EDTA was then added to the mix and incubated at 65 • C for 10 min to inactivate the DNase I enzyme.

    Article Title: Tissue-type transglutaminase and the effects of cystamine on intracerebral hemorrhage-induced brain edema and neurological deficits
    Article Snippet: .. Total RNA was extracted with Trizol reagent (Gibco BRL Grand Island, NY, U.S.A.), and 1µg RNA was digested with amplification-grade deoxyribonuclease I (Gibco BRL). .. Complementary DNA was synthesized by reverse transcription using the digested 1µg RNA (11µL) with 14 µL reaction buffer (Perkin Elmer, Foster City, CA, U.S.A.) containing dNTP (dATP, dCTP, dGTP and dTTP), 25mmol/L magnesium chloride, 10x polymerase chain reaction buffer II, Random Hexamer Primer, ribonuclease inhibitor, and murine leukemia virus reverse transcriptase.

    Article Title: Coordinated Expression of Dopamine Receptors in Neostriatal Medium Spiny Neurons
    Article Snippet: .. Before RT-PCR, the isolated RNA was treated with amplification grade DNase I (Life Technologies) to eliminate genomic DNA. ..

    Article Title: Identification of Redox Partners of the Thiol-Disulfide Oxidoreductase SdbA in Streptococcus gordonii
    Article Snippet: .. DNA was removed from RNA (1 μg) samples by digestion with amplification grade DNase I (Life Technologies) for 15 min at room temperature. .. The resulting RNA samples were confirmed to be DNA-free by performing PCR for the 16S rRNA gene using primers SL525/SL697. cDNA was synthesized from the RNA using random primers and SuperScript II reverse transcriptase (Life Technologies) according to the manufacturer’s instructions.

    Article Title: EVIDENCE FOR THE EXPRESSION OF PARATHYROID HORMONE 2 RECEPTOR IN THE HUMAN BRAINSTEM
    Article Snippet: .. RNA was treated with Amplification Grade DNase I (Invitrogen) and cDNA was synthesized with a Superscript II reverse transcriptase kit (Invitrogen) according to the manufacturer’s instructions. ..

    Article Title: Infection drives meningeal engraftment by inflammatory monocytes that impairs CNS immunity
    Article Snippet: .. Purified RNA was then treated with amplification-grade DNase I (Invitrogen) to remove contaminating DNA and reverse transcribed into cDNA by using an iScript cDNA Synthesis kit (Life Technologies). .. Real-time PCR was performed using SYBR Green (Applied Biosystems) and cDNA template or water (non-template negative control) at an annealing temperature of 60 degrees with a CFX96 Real-Time PCR machine (Bio-Rad Laboratories).

    Article Title: Antiviral activity of zinc salts against transmissible gastroenteritis virus in vitro.
    Article Snippet: .. RNA was eluted in 50 ml RNase-free water and treated with Amplification Grade DNase I (Invitrogen) according to the manufacturer's instructions. .. Total RNA was reverse transcribed using the RevertAidTM First Strand cDNA Synthesis Kit (Fermentas) with oligo(dT) and random hexamer primers.

    Article Title: LIM and cysteine-rich domains 1 (LMCD1) regulates skeletal muscle hypertrophy, calcium handling, and force
    Article Snippet: .. Afterward, 1 μg of RNA was treated with Amplification Grade DNase I (Life Technologies) and from that, 500 ng were used for cDNA preparation using the Applied Biosystem Reverse Transcription Kit (Life Technologies). .. Quantitative real-time PCR was performed in a ViiA 7 Real-Time PCR system thermal cycler with SYBR Green PCR Master Mix (both Applied Biosystems).

    Synthesized:

    Article Title: Tissue-type transglutaminase and the effects of cystamine on intracerebral hemorrhage-induced brain edema and neurological deficits
    Article Snippet: Total RNA was extracted with Trizol reagent (Gibco BRL Grand Island, NY, U.S.A.), and 1µg RNA was digested with amplification-grade deoxyribonuclease I (Gibco BRL). .. Complementary DNA was synthesized by reverse transcription using the digested 1µg RNA (11µL) with 14 µL reaction buffer (Perkin Elmer, Foster City, CA, U.S.A.) containing dNTP (dATP, dCTP, dGTP and dTTP), 25mmol/L magnesium chloride, 10x polymerase chain reaction buffer II, Random Hexamer Primer, ribonuclease inhibitor, and murine leukemia virus reverse transcriptase.

    Article Title: Identification of Redox Partners of the Thiol-Disulfide Oxidoreductase SdbA in Streptococcus gordonii
    Article Snippet: DNA was removed from RNA (1 μg) samples by digestion with amplification grade DNase I (Life Technologies) for 15 min at room temperature. .. The resulting RNA samples were confirmed to be DNA-free by performing PCR for the 16S rRNA gene using primers SL525/SL697. cDNA was synthesized from the RNA using random primers and SuperScript II reverse transcriptase (Life Technologies) according to the manufacturer’s instructions.

    Article Title: EVIDENCE FOR THE EXPRESSION OF PARATHYROID HORMONE 2 RECEPTOR IN THE HUMAN BRAINSTEM
    Article Snippet: .. RNA was treated with Amplification Grade DNase I (Invitrogen) and cDNA was synthesized with a Superscript II reverse transcriptase kit (Invitrogen) according to the manufacturer’s instructions. ..

    Mutagenesis:

    Article Title: Identification of Redox Partners of the Thiol-Disulfide Oxidoreductase SdbA in Streptococcus gordonii
    Article Snippet: DNA was removed from RNA (1 μg) samples by digestion with amplification grade DNase I (Life Technologies) for 15 min at room temperature. .. The cDNA was also used in qRT-PCR for the expression of sgo_1170 and sgo_1174 in the parent, sdbB-ccdA2 mutant, and sdbB-ccdA2 -knock-in mutant. qRT-PCR was performed using the PowerUp Sybr green master mix (Invitrogen) on an Applied Biosystems StepOne machine with a relative standard curve analysis.

    Isolation:

    Article Title: Coordinated Expression of Dopamine Receptors in Neostriatal Medium Spiny Neurons
    Article Snippet: .. Before RT-PCR, the isolated RNA was treated with amplification grade DNase I (Life Technologies) to eliminate genomic DNA. ..

    Article Title: Antiviral activity of zinc salts against transmissible gastroenteritis virus in vitro.
    Article Snippet: Real-time PCR quantification of viral RNA synthesis For the relative quantification of viral RNA synthesis, ST cells were infected with TGEV for 1 h and then treated with Zn or control salt at a concentration of 100 mM for additional 4 h. Cells were harvested after 48 h, and total RNA was isolated using the Invisorb Spin Cell RNA Mini Kit (Invitek). .. RNA was eluted in 50 ml RNase-free water and treated with Amplification Grade DNase I (Invitrogen) according to the manufacturer's instructions.

    Article Title: LIM and cysteine-rich domains 1 (LMCD1) regulates skeletal muscle hypertrophy, calcium handling, and force
    Article Snippet: Gene expression analysis Total RNA was isolated using Isol-RNA Lysis Reagent (5 PRIME), according to manufacturer’s instructions. .. Afterward, 1 μg of RNA was treated with Amplification Grade DNase I (Life Technologies) and from that, 500 ng were used for cDNA preparation using the Applied Biosystem Reverse Transcription Kit (Life Technologies).

    Polymerase Chain Reaction:

    Article Title: Tissue-type transglutaminase and the effects of cystamine on intracerebral hemorrhage-induced brain edema and neurological deficits
    Article Snippet: Total RNA was extracted with Trizol reagent (Gibco BRL Grand Island, NY, U.S.A.), and 1µg RNA was digested with amplification-grade deoxyribonuclease I (Gibco BRL). .. Complementary DNA was synthesized by reverse transcription using the digested 1µg RNA (11µL) with 14 µL reaction buffer (Perkin Elmer, Foster City, CA, U.S.A.) containing dNTP (dATP, dCTP, dGTP and dTTP), 25mmol/L magnesium chloride, 10x polymerase chain reaction buffer II, Random Hexamer Primer, ribonuclease inhibitor, and murine leukemia virus reverse transcriptase.

    Article Title: Coordinated Expression of Dopamine Receptors in Neostriatal Medium Spiny Neurons
    Article Snippet: Before RT-PCR, the isolated RNA was treated with amplification grade DNase I (Life Technologies) to eliminate genomic DNA. .. Conventional PCR analysis of dopamine receptor cDNAs was performed as described above.

    Article Title: Identification of Redox Partners of the Thiol-Disulfide Oxidoreductase SdbA in Streptococcus gordonii
    Article Snippet: DNA was removed from RNA (1 μg) samples by digestion with amplification grade DNase I (Life Technologies) for 15 min at room temperature. .. The resulting RNA samples were confirmed to be DNA-free by performing PCR for the 16S rRNA gene using primers SL525/SL697. cDNA was synthesized from the RNA using random primers and SuperScript II reverse transcriptase (Life Technologies) according to the manufacturer’s instructions.

    Article Title: EVIDENCE FOR THE EXPRESSION OF PARATHYROID HORMONE 2 RECEPTOR IN THE HUMAN BRAINSTEM
    Article Snippet: RNA was treated with Amplification Grade DNase I (Invitrogen) and cDNA was synthesized with a Superscript II reverse transcriptase kit (Invitrogen) according to the manufacturer’s instructions. .. The resulting cDNA was used as template in PCR reactions performed with iTaq DNA polymerase (Bio-Rad Laboratories, Hercules, CA).

    Article Title: LIM and cysteine-rich domains 1 (LMCD1) regulates skeletal muscle hypertrophy, calcium handling, and force
    Article Snippet: Afterward, 1 μg of RNA was treated with Amplification Grade DNase I (Life Technologies) and from that, 500 ng were used for cDNA preparation using the Applied Biosystem Reverse Transcription Kit (Life Technologies). .. Quantitative real-time PCR was performed in a ViiA 7 Real-Time PCR system thermal cycler with SYBR Green PCR Master Mix (both Applied Biosystems).

    Incubation:

    Article Title: Ribosomal RNA depletion or exclusion has negligible effect on the detection of viruses in a pan viral microarray.
    Article Snippet: Nucleic acid was quantified using Nanodrop 2000 spectrometer (Agilent Technologies, Cheshire, UK) and diluted to a concentration of 4 g in 32 l of nuclease free water, from which three aliquots of 8 l were subjected to DNase digest using amplification grade DNase I (Life Technologies, Paisley, UK). .. Briefly, 1 l of 10× DNase buffer and 1 l of DNase I enzyme (1 units/l) were added to each 8 l nucleic acid extract and incubated at 37 • C for 30 min. 1 l of 25 mM EDTA was then added to the mix and incubated at 65 • C for 10 min to inactivate the DNase I enzyme.

    Quantitative RT-PCR:

    Article Title: Identification of Redox Partners of the Thiol-Disulfide Oxidoreductase SdbA in Streptococcus gordonii
    Article Snippet: DNA was removed from RNA (1 μg) samples by digestion with amplification grade DNase I (Life Technologies) for 15 min at room temperature. .. The cDNA was also used in qRT-PCR for the expression of sgo_1170 and sgo_1174 in the parent, sdbB-ccdA2 mutant, and sdbB-ccdA2 -knock-in mutant. qRT-PCR was performed using the PowerUp Sybr green master mix (Invitrogen) on an Applied Biosystems StepOne machine with a relative standard curve analysis.

    Concentration Assay:

    Article Title: Ribosomal RNA depletion or exclusion has negligible effect on the detection of viruses in a pan viral microarray.
    Article Snippet: .. Nucleic acid was quantified using Nanodrop 2000 spectrometer (Agilent Technologies, Cheshire, UK) and diluted to a concentration of 4 g in 32 l of nuclease free water, from which three aliquots of 8 l were subjected to DNase digest using amplification grade DNase I (Life Technologies, Paisley, UK). .. Briefly, 1 l of 10× DNase buffer and 1 l of DNase I enzyme (1 units/l) were added to each 8 l nucleic acid extract and incubated at 37 • C for 30 min. 1 l of 25 mM EDTA was then added to the mix and incubated at 65 • C for 10 min to inactivate the DNase I enzyme.

    Article Title: Antiviral activity of zinc salts against transmissible gastroenteritis virus in vitro.
    Article Snippet: Real-time PCR quantification of viral RNA synthesis For the relative quantification of viral RNA synthesis, ST cells were infected with TGEV for 1 h and then treated with Zn or control salt at a concentration of 100 mM for additional 4 h. Cells were harvested after 48 h, and total RNA was isolated using the Invisorb Spin Cell RNA Mini Kit (Invitek). .. RNA was eluted in 50 ml RNase-free water and treated with Amplification Grade DNase I (Invitrogen) according to the manufacturer's instructions.

    SYBR Green Assay:

    Article Title: Identification of Redox Partners of the Thiol-Disulfide Oxidoreductase SdbA in Streptococcus gordonii
    Article Snippet: DNA was removed from RNA (1 μg) samples by digestion with amplification grade DNase I (Life Technologies) for 15 min at room temperature. .. The cDNA was also used in qRT-PCR for the expression of sgo_1170 and sgo_1174 in the parent, sdbB-ccdA2 mutant, and sdbB-ccdA2 -knock-in mutant. qRT-PCR was performed using the PowerUp Sybr green master mix (Invitrogen) on an Applied Biosystems StepOne machine with a relative standard curve analysis.

    Article Title: Infection drives meningeal engraftment by inflammatory monocytes that impairs CNS immunity
    Article Snippet: Purified RNA was then treated with amplification-grade DNase I (Invitrogen) to remove contaminating DNA and reverse transcribed into cDNA by using an iScript cDNA Synthesis kit (Life Technologies). .. Real-time PCR was performed using SYBR Green (Applied Biosystems) and cDNA template or water (non-template negative control) at an annealing temperature of 60 degrees with a CFX96 Real-Time PCR machine (Bio-Rad Laboratories).

    Article Title: LIM and cysteine-rich domains 1 (LMCD1) regulates skeletal muscle hypertrophy, calcium handling, and force
    Article Snippet: Afterward, 1 μg of RNA was treated with Amplification Grade DNase I (Life Technologies) and from that, 500 ng were used for cDNA preparation using the Applied Biosystem Reverse Transcription Kit (Life Technologies). .. Quantitative real-time PCR was performed in a ViiA 7 Real-Time PCR system thermal cycler with SYBR Green PCR Master Mix (both Applied Biosystems).

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Coordinated Expression of Dopamine Receptors in Neostriatal Medium Spiny Neurons
    Article Snippet: .. Before RT-PCR, the isolated RNA was treated with amplification grade DNase I (Life Technologies) to eliminate genomic DNA. ..

    Article Title: Identification of Redox Partners of the Thiol-Disulfide Oxidoreductase SdbA in Streptococcus gordonii
    Article Snippet: DNA was removed from RNA (1 μg) samples by digestion with amplification grade DNase I (Life Technologies) for 15 min at room temperature. .. The resulting cDNA was used as the template in RT-PCR for the analysis of the sgo_1177-ccdA1 and sdbB-ccdA2 operons.

    Article Title: EVIDENCE FOR THE EXPRESSION OF PARATHYROID HORMONE 2 RECEPTOR IN THE HUMAN BRAINSTEM
    Article Snippet: Paragraph title: RT-PCR ... RNA was treated with Amplification Grade DNase I (Invitrogen) and cDNA was synthesized with a Superscript II reverse transcriptase kit (Invitrogen) according to the manufacturer’s instructions.

    Random Hexamer Labeling:

    Article Title: Tissue-type transglutaminase and the effects of cystamine on intracerebral hemorrhage-induced brain edema and neurological deficits
    Article Snippet: Total RNA was extracted with Trizol reagent (Gibco BRL Grand Island, NY, U.S.A.), and 1µg RNA was digested with amplification-grade deoxyribonuclease I (Gibco BRL). .. Complementary DNA was synthesized by reverse transcription using the digested 1µg RNA (11µL) with 14 µL reaction buffer (Perkin Elmer, Foster City, CA, U.S.A.) containing dNTP (dATP, dCTP, dGTP and dTTP), 25mmol/L magnesium chloride, 10x polymerase chain reaction buffer II, Random Hexamer Primer, ribonuclease inhibitor, and murine leukemia virus reverse transcriptase.

    Article Title: Antiviral activity of zinc salts against transmissible gastroenteritis virus in vitro.
    Article Snippet: RNA was eluted in 50 ml RNase-free water and treated with Amplification Grade DNase I (Invitrogen) according to the manufacturer's instructions. .. Total RNA was reverse transcribed using the RevertAidTM First Strand cDNA Synthesis Kit (Fermentas) with oligo(dT) and random hexamer primers.

    Selection:

    Article Title: Ribosomal RNA depletion or exclusion has negligible effect on the detection of viruses in a pan viral microarray.
    Article Snippet: Samples and nucleic acid extraction A selection of virus positive tissue samples was used in this study (Table 1) . .. Nucleic acid was quantified using Nanodrop 2000 spectrometer (Agilent Technologies, Cheshire, UK) and diluted to a concentration of 4 g in 32 l of nuclease free water, from which three aliquots of 8 l were subjected to DNase digest using amplification grade DNase I (Life Technologies, Paisley, UK).

    Infection:

    Article Title: Antiviral activity of zinc salts against transmissible gastroenteritis virus in vitro.
    Article Snippet: Real-time PCR quantification of viral RNA synthesis For the relative quantification of viral RNA synthesis, ST cells were infected with TGEV for 1 h and then treated with Zn or control salt at a concentration of 100 mM for additional 4 h. Cells were harvested after 48 h, and total RNA was isolated using the Invisorb Spin Cell RNA Mini Kit (Invitek). .. RNA was eluted in 50 ml RNase-free water and treated with Amplification Grade DNase I (Invitrogen) according to the manufacturer's instructions.

    Expressing:

    Article Title: Identification of Redox Partners of the Thiol-Disulfide Oxidoreductase SdbA in Streptococcus gordonii
    Article Snippet: DNA was removed from RNA (1 μg) samples by digestion with amplification grade DNase I (Life Technologies) for 15 min at room temperature. .. The cDNA was also used in qRT-PCR for the expression of sgo_1170 and sgo_1174 in the parent, sdbB-ccdA2 mutant, and sdbB-ccdA2 -knock-in mutant. qRT-PCR was performed using the PowerUp Sybr green master mix (Invitrogen) on an Applied Biosystems StepOne machine with a relative standard curve analysis.

    Article Title: Infection drives meningeal engraftment by inflammatory monocytes that impairs CNS immunity
    Article Snippet: Purified RNA was then treated with amplification-grade DNase I (Invitrogen) to remove contaminating DNA and reverse transcribed into cDNA by using an iScript cDNA Synthesis kit (Life Technologies). .. For each gene, expression values were normalized to a Actb (meningeal samples) or 18S (sorted cells) housekeeping genes.

    Article Title: Antiviral activity of zinc salts against transmissible gastroenteritis virus in vitro.
    Article Snippet: RNA was eluted in 50 ml RNase-free water and treated with Amplification Grade DNase I (Invitrogen) according to the manufacturer's instructions. .. The relative expression of the TGEV S protein gene (forward, 5 0 -GTATTGGGATTATGCT-3 0 ; reverse, 5 0 -GGTGGTGGTAGTAGGT-3 0 ) was determined by normalizing against the porcine b-actin gene (forward, 5 0 -GGACTTCGAGCAGGAGATGG-3 0 ; reverse, 5 0 -GCACCGTG-TTGGCGTAGAGG-3 0 ).

    Article Title: LIM and cysteine-rich domains 1 (LMCD1) regulates skeletal muscle hypertrophy, calcium handling, and force
    Article Snippet: Paragraph title: Gene expression analysis ... Afterward, 1 μg of RNA was treated with Amplification Grade DNase I (Life Technologies) and from that, 500 ng were used for cDNA preparation using the Applied Biosystem Reverse Transcription Kit (Life Technologies).

    FACS:

    Article Title: Infection drives meningeal engraftment by inflammatory monocytes that impairs CNS immunity
    Article Snippet: Meningeal tissue or FACS-sorted cells were collected in TRIzol (Invitrogen), and total RNA was extracted using PureLink RNA Mini or Micro kits (Life Technologies) per the manufacturer’s instructions. .. Purified RNA was then treated with amplification-grade DNase I (Invitrogen) to remove contaminating DNA and reverse transcribed into cDNA by using an iScript cDNA Synthesis kit (Life Technologies).

    Knock-In:

    Article Title: Identification of Redox Partners of the Thiol-Disulfide Oxidoreductase SdbA in Streptococcus gordonii
    Article Snippet: DNA was removed from RNA (1 μg) samples by digestion with amplification grade DNase I (Life Technologies) for 15 min at room temperature. .. The cDNA was also used in qRT-PCR for the expression of sgo_1170 and sgo_1174 in the parent, sdbB-ccdA2 mutant, and sdbB-ccdA2 -knock-in mutant. qRT-PCR was performed using the PowerUp Sybr green master mix (Invitrogen) on an Applied Biosystems StepOne machine with a relative standard curve analysis.

    Lysis:

    Article Title: LIM and cysteine-rich domains 1 (LMCD1) regulates skeletal muscle hypertrophy, calcium handling, and force
    Article Snippet: Gene expression analysis Total RNA was isolated using Isol-RNA Lysis Reagent (5 PRIME), according to manufacturer’s instructions. .. Afterward, 1 μg of RNA was treated with Amplification Grade DNase I (Life Technologies) and from that, 500 ng were used for cDNA preparation using the Applied Biosystem Reverse Transcription Kit (Life Technologies).

    Purification:

    Article Title: EVIDENCE FOR THE EXPRESSION OF PARATHYROID HORMONE 2 RECEPTOR IN THE HUMAN BRAINSTEM
    Article Snippet: The degradation of RNA was assessed by running the purified RNAs on denaturing formaldehyde gels. .. RNA was treated with Amplification Grade DNase I (Invitrogen) and cDNA was synthesized with a Superscript II reverse transcriptase kit (Invitrogen) according to the manufacturer’s instructions.

    Article Title: Infection drives meningeal engraftment by inflammatory monocytes that impairs CNS immunity
    Article Snippet: .. Purified RNA was then treated with amplification-grade DNase I (Invitrogen) to remove contaminating DNA and reverse transcribed into cDNA by using an iScript cDNA Synthesis kit (Life Technologies). .. Real-time PCR was performed using SYBR Green (Applied Biosystems) and cDNA template or water (non-template negative control) at an annealing temperature of 60 degrees with a CFX96 Real-Time PCR machine (Bio-Rad Laboratories).

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Thermo Fisher dnase i amplification grade
    Overview of RT-RamDA and single-cell RamDA-seq.  a  Schematic diagram of RT-RamDA. 1. RT primers (oligo-dT and not-so-random primers) anneal to a RNA template. 2. Complementary DNA (cDNA) is synthesized by the RNA-dependent DNA polymerase activity of RNase H minus reverse transcriptase (RTase). 3. Endonuclease (DNase I) selectively nicks the cDNA of the RNA:cDNA hybrid strand. 4. The 3′ cDNA strand is displaced by the strand displacement activity of RTase mediated by the T4 gene 32 protein (gp32), starting from the nick randomly introduced by DNase I. cDNA is amplified as a displaced strand and protected by gp32 from DNase I.  b  Relative yield of cDNA molecules using RT-qPCR ( n  = 4). Mouse ESC total RNA (10 pg) was used as a template, and 1/10 the amount of cDNA was used for qPCR. The relative yield was calculated by averaging the amplification efficiency of four mESC ( Nanog ,  Pou5f1 ,  Zfp42 , and  Sox2 ) and three housekeeping ( Gnb2l1 ,  Atp5a1 , and  Tubb5 ) genes using a conventional method (−) as a standard.  c  Schematic diagram of RamDA-seq and C1-RamDA-seq. For details, please refer to the Methods section.  d  Number of detected transcripts with twofold or lower expression changes against rdRNA-seq (count ≥ 10). For the boxplots in  b  and  d , the center line, and lower and upper bounds of each box represent the median, and first and third quartiles, respectively. The lower (upper) whisker extends to smallest (largest) values no further than 1.5 × interquartile range (IQR) from the first (third) quartile.  e  Squared coefficient of variation of the read count. All conditions were adjusted, and 10 million reads were used in  d  and  e . Transcripts were annotated by GENCODE gene annotation (vM9)
    Dnase I Amplification Grade, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dnase i amplification grade/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dnase i amplification grade - by Bioz Stars, 2020-03
    99/100 stars
      Buy from Supplier

    Image Search Results


    Overview of RT-RamDA and single-cell RamDA-seq.  a  Schematic diagram of RT-RamDA. 1. RT primers (oligo-dT and not-so-random primers) anneal to a RNA template. 2. Complementary DNA (cDNA) is synthesized by the RNA-dependent DNA polymerase activity of RNase H minus reverse transcriptase (RTase). 3. Endonuclease (DNase I) selectively nicks the cDNA of the RNA:cDNA hybrid strand. 4. The 3′ cDNA strand is displaced by the strand displacement activity of RTase mediated by the T4 gene 32 protein (gp32), starting from the nick randomly introduced by DNase I. cDNA is amplified as a displaced strand and protected by gp32 from DNase I.  b  Relative yield of cDNA molecules using RT-qPCR ( n  = 4). Mouse ESC total RNA (10 pg) was used as a template, and 1/10 the amount of cDNA was used for qPCR. The relative yield was calculated by averaging the amplification efficiency of four mESC ( Nanog ,  Pou5f1 ,  Zfp42 , and  Sox2 ) and three housekeeping ( Gnb2l1 ,  Atp5a1 , and  Tubb5 ) genes using a conventional method (−) as a standard.  c  Schematic diagram of RamDA-seq and C1-RamDA-seq. For details, please refer to the Methods section.  d  Number of detected transcripts with twofold or lower expression changes against rdRNA-seq (count ≥ 10). For the boxplots in  b  and  d , the center line, and lower and upper bounds of each box represent the median, and first and third quartiles, respectively. The lower (upper) whisker extends to smallest (largest) values no further than 1.5 × interquartile range (IQR) from the first (third) quartile.  e  Squared coefficient of variation of the read count. All conditions were adjusted, and 10 million reads were used in  d  and  e . Transcripts were annotated by GENCODE gene annotation (vM9)

    Journal: Nature Communications

    Article Title: Single-cell full-length total RNA sequencing uncovers dynamics of recursive splicing and enhancer RNAs

    doi: 10.1038/s41467-018-02866-0

    Figure Lengend Snippet: Overview of RT-RamDA and single-cell RamDA-seq. a Schematic diagram of RT-RamDA. 1. RT primers (oligo-dT and not-so-random primers) anneal to a RNA template. 2. Complementary DNA (cDNA) is synthesized by the RNA-dependent DNA polymerase activity of RNase H minus reverse transcriptase (RTase). 3. Endonuclease (DNase I) selectively nicks the cDNA of the RNA:cDNA hybrid strand. 4. The 3′ cDNA strand is displaced by the strand displacement activity of RTase mediated by the T4 gene 32 protein (gp32), starting from the nick randomly introduced by DNase I. cDNA is amplified as a displaced strand and protected by gp32 from DNase I. b Relative yield of cDNA molecules using RT-qPCR ( n  = 4). Mouse ESC total RNA (10 pg) was used as a template, and 1/10 the amount of cDNA was used for qPCR. The relative yield was calculated by averaging the amplification efficiency of four mESC ( Nanog , Pou5f1 , Zfp42 , and Sox2 ) and three housekeeping ( Gnb2l1 , Atp5a1 , and Tubb5 ) genes using a conventional method (−) as a standard. c Schematic diagram of RamDA-seq and C1-RamDA-seq. For details, please refer to the Methods section. d Number of detected transcripts with twofold or lower expression changes against rdRNA-seq (count ≥ 10). For the boxplots in b and d , the center line, and lower and upper bounds of each box represent the median, and first and third quartiles, respectively. The lower (upper) whisker extends to smallest (largest) values no further than 1.5 × interquartile range (IQR) from the first (third) quartile. e Squared coefficient of variation of the read count. All conditions were adjusted, and 10 million reads were used in d and e . Transcripts were annotated by GENCODE gene annotation (vM9)

    Article Snippet: A mixture containing 2 μL of conventional RT mix (1.5× PrimeScript buffer, 0.6 pmol oligo(dT)18 (Thermo Fisher), 8 pmol random hexamers (TaKaRa), and 1.5× PrimeScript enzyme mix in RNase-free water) or 2 μL of RT-RamDA mix (1.5× PrimeScript buffer, 0.6 pmol oligo(dT)18, 8 pmol random hexamers or NSRs, 0.2 U of DNase I Amplification Grade (Thermo Fisher), 100 ng of T4 gene 32 protein (Roche), and 1.5× PrimeScript enzyme mix in RNase-free water) was added to 1 μL of diluted, denatured template RNA.

    Techniques: Synthesized, Activity Assay, Amplification, Quantitative RT-PCR, Real-time Polymerase Chain Reaction, Expressing, Whisker Assay

    NETs contribute to the pathogenesis of myocarditis. (a) Confocal immunofluorescence images of an EMB of patient 6 suffering from PVB19-positive myocarditis (see Table 1 ). Top: Images show staining of H2A-H2B-DNA, MPO, and H3Cit as well as the merged image. Bar, 20 µm. Bottom: Magnification of a selected area of the merged confocal image (box) from the top panel. Arrows indicate NETs. Bar, 10 µm. (b and c) EAM score (b) as well as evaluation of the heart/body weight ratio (c) at day 21 after induction of EAM or sham immunization (sham; mean EAM score, 0.5 ± 0.19; mean heart/body weight ratio, 0.57 ± 0.01). Mice were treated with DNase (EAM + DNase: mean EAM score, 0.8 ± 0.19; mean heart/body weight ratio, 0.62 ± 0.005), Cl-amid (EAM + Cl-amid: mean EAM score, 0.9 ± 0.20; mean heart/body weight ratio, 0.62 ± 0.01), or vehicle as control (EAM + vehicle: mean EAM score, 1.7 ± 0.25; mean heart/body weight ratio, 0.71 ± 0.04) as indicated. n = 8 for sham group; n = 26 for EAM groups. (d) Left: Representative confocal images of cardiac sections of immunized WT mice at day 21 after induction of EAM (EAM + vehicle). Images show staining of DNA, NE, and H3Cit as well as the merged image. Bars, 20 µm. Right: Representative confocal magnified image of NETs. Images show staining of DNA, NE, and H3Cit as well as the merged image, colocalization (coloc) of DNA/NE, and DNA/H3Cit as indicated. Bars, 10 µm. (e) Representative confocal images of cardiac sections of EAM mice at day 21 after induction of EAM and blockade of MK for 21 d. Images show staining of DNA, NE, and H3Cit as well as the merged image. Bars, 20 µm. (f–h) Histological analysis of extravasated PMN into the cardiac tissue at day 21 after induction of EAM without blocking MK (EAM + vehicle) or after application of an anti-N-MK blocking Ab (EAM + anti-N-MK). Diagrams show the number of extravasated PMNs per mm 2 (f: vehicle, 768 ± 55 PMN/mm 2 ; anti-N-MK, 158 ± 86 PMN/mm 2 ), the number of NET + PMNs per mm 2 (g: vehicle, 40 ± 6 NET + PMN; anti-N-MK = 5 ± 2 NET + PMN), and the number of NET + PMNs as a percentage of all extravasated PMNs (h: vehicle, 5.1 ± 0.5 NET + PMN [%]; anti-N-MK, 0.9 ± 0.6 NET + PMN [%]). n = 6 mice. To determine P values, ANOVA on ranks with Dunn’s multiple comparisons test was performed for c and d, and an unpaired Student's t test was used for f–h. For all panels, *, P

    Journal: The Journal of Experimental Medicine

    Article Title: Midkine drives cardiac inflammation by promoting neutrophil trafficking and NETosis in myocarditis

    doi: 10.1084/jem.20181102

    Figure Lengend Snippet: NETs contribute to the pathogenesis of myocarditis. (a) Confocal immunofluorescence images of an EMB of patient 6 suffering from PVB19-positive myocarditis (see Table 1 ). Top: Images show staining of H2A-H2B-DNA, MPO, and H3Cit as well as the merged image. Bar, 20 µm. Bottom: Magnification of a selected area of the merged confocal image (box) from the top panel. Arrows indicate NETs. Bar, 10 µm. (b and c) EAM score (b) as well as evaluation of the heart/body weight ratio (c) at day 21 after induction of EAM or sham immunization (sham; mean EAM score, 0.5 ± 0.19; mean heart/body weight ratio, 0.57 ± 0.01). Mice were treated with DNase (EAM + DNase: mean EAM score, 0.8 ± 0.19; mean heart/body weight ratio, 0.62 ± 0.005), Cl-amid (EAM + Cl-amid: mean EAM score, 0.9 ± 0.20; mean heart/body weight ratio, 0.62 ± 0.01), or vehicle as control (EAM + vehicle: mean EAM score, 1.7 ± 0.25; mean heart/body weight ratio, 0.71 ± 0.04) as indicated. n = 8 for sham group; n = 26 for EAM groups. (d) Left: Representative confocal images of cardiac sections of immunized WT mice at day 21 after induction of EAM (EAM + vehicle). Images show staining of DNA, NE, and H3Cit as well as the merged image. Bars, 20 µm. Right: Representative confocal magnified image of NETs. Images show staining of DNA, NE, and H3Cit as well as the merged image, colocalization (coloc) of DNA/NE, and DNA/H3Cit as indicated. Bars, 10 µm. (e) Representative confocal images of cardiac sections of EAM mice at day 21 after induction of EAM and blockade of MK for 21 d. Images show staining of DNA, NE, and H3Cit as well as the merged image. Bars, 20 µm. (f–h) Histological analysis of extravasated PMN into the cardiac tissue at day 21 after induction of EAM without blocking MK (EAM + vehicle) or after application of an anti-N-MK blocking Ab (EAM + anti-N-MK). Diagrams show the number of extravasated PMNs per mm 2 (f: vehicle, 768 ± 55 PMN/mm 2 ; anti-N-MK, 158 ± 86 PMN/mm 2 ), the number of NET + PMNs per mm 2 (g: vehicle, 40 ± 6 NET + PMN; anti-N-MK = 5 ± 2 NET + PMN), and the number of NET + PMNs as a percentage of all extravasated PMNs (h: vehicle, 5.1 ± 0.5 NET + PMN [%]; anti-N-MK, 0.9 ± 0.6 NET + PMN [%]). n = 6 mice. To determine P values, ANOVA on ranks with Dunn’s multiple comparisons test was performed for c and d, and an unpaired Student's t test was used for f–h. For all panels, *, P

    Article Snippet: RNA was isolated using TRIzol (15596026; Thermo Fisher) according to the manufacturer’s protocol, and genomic DNA was digested using the DNase I Amplification Grade kit (18068015; Thermo Fisher).

    Techniques: Immunofluorescence, Staining, Mouse Assay, Blocking Assay