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Roche amplicor hcv rna
Mean values of results (expressed as log 10 <t>HCV</t> <t>RNA</t> copies per milliliter of serum) determined by our RT-PCR protocol (ABI Prism 7700 assay), the bDNA assay and the NGI method are comparable. (A) Comparison of totals; differences in mean values are not significant (ns). The percentages of samples positive by each method are given in the box. (B) Comparison of mean values for groups of different genotypes; differences are not significant.
Amplicor Hcv Rna, supplied by Roche, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/amplicor hcv rna/product/Roche
Average 85 stars, based on 1 article reviews
Price from $9.99 to $1999.99
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1) Product Images from "High-Throughput Real-Time Reverse Transcription-PCR Quantitation of Hepatitis C Virus RNA"

Article Title: High-Throughput Real-Time Reverse Transcription-PCR Quantitation of Hepatitis C Virus RNA

Journal: Journal of Clinical Microbiology

doi:

Mean values of results (expressed as log 10 HCV RNA copies per milliliter of serum) determined by our RT-PCR protocol (ABI Prism 7700 assay), the bDNA assay and the NGI method are comparable. (A) Comparison of totals; differences in mean values are not significant (ns). The percentages of samples positive by each method are given in the box. (B) Comparison of mean values for groups of different genotypes; differences are not significant.
Figure Legend Snippet: Mean values of results (expressed as log 10 HCV RNA copies per milliliter of serum) determined by our RT-PCR protocol (ABI Prism 7700 assay), the bDNA assay and the NGI method are comparable. (A) Comparison of totals; differences in mean values are not significant (ns). The percentages of samples positive by each method are given in the box. (B) Comparison of mean values for groups of different genotypes; differences are not significant.

Techniques Used: Reverse Transcription Polymerase Chain Reaction

Schematic representation of the transcription fragment from the 5′ NCR of HCV. Oligonucleotide primers C149 and C342 were used for amplification by RT-PCR. Primers C24 and C339 were hybridized to the RNA transcript to assess its integrity. FT275 is the dual-labeled probe. R, reporter; Q, quencher; nt, nucleotides.
Figure Legend Snippet: Schematic representation of the transcription fragment from the 5′ NCR of HCV. Oligonucleotide primers C149 and C342 were used for amplification by RT-PCR. Primers C24 and C339 were hybridized to the RNA transcript to assess its integrity. FT275 is the dual-labeled probe. R, reporter; Q, quencher; nt, nucleotides.

Techniques Used: Amplification, Reverse Transcription Polymerase Chain Reaction, Labeling

Standard curve of the input RNA concentration in serial dilutions of the first reference standard versus C T . Each dot represents the result of triplicate PCR amplifications for each dilution. The stars represent the results of PCR amplification of samples containing unknown quantities of HCV RNA tested by this method. R was equal to 0.999, and the slope was −3.429.
Figure Legend Snippet: Standard curve of the input RNA concentration in serial dilutions of the first reference standard versus C T . Each dot represents the result of triplicate PCR amplifications for each dilution. The stars represent the results of PCR amplification of samples containing unknown quantities of HCV RNA tested by this method. R was equal to 0.999, and the slope was −3.429.

Techniques Used: Concentration Assay, Polymerase Chain Reaction, Amplification

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    Roche amplicor hcv rna
    Mean values of results (expressed as log 10 <t>HCV</t> <t>RNA</t> copies per milliliter of serum) determined by our RT-PCR protocol (ABI Prism 7700 assay), the bDNA assay and the NGI method are comparable. (A) Comparison of totals; differences in mean values are not significant (ns). The percentages of samples positive by each method are given in the box. (B) Comparison of mean values for groups of different genotypes; differences are not significant.
    Amplicor Hcv Rna, supplied by Roche, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/amplicor hcv rna/product/Roche
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    amplicor hcv rna - by Bioz Stars, 2020-05
    85/100 stars
      Buy from Supplier

    92
    Roche hcv rna
    The timeline summary of the case. <t>HCV</t> = hepatitis C virus, HIV = human immunodeficiency virus, <t>RNA</t> = ribonucleic acid. a Cobas Amplicor HCV Monitor test, v2.0, Roche Switzerland, b Abbott RealTi m e HCV assay, c Xpert HCV Viral Load.
    Hcv Rna, supplied by Roche, used in various techniques. Bioz Stars score: 92/100, based on 152 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hcv rna/product/Roche
    Average 92 stars, based on 152 article reviews
    Price from $9.99 to $1999.99
    hcv rna - by Bioz Stars, 2020-05
    92/100 stars
      Buy from Supplier

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    Mean values of results (expressed as log 10 HCV RNA copies per milliliter of serum) determined by our RT-PCR protocol (ABI Prism 7700 assay), the bDNA assay and the NGI method are comparable. (A) Comparison of totals; differences in mean values are not significant (ns). The percentages of samples positive by each method are given in the box. (B) Comparison of mean values for groups of different genotypes; differences are not significant.

    Journal: Journal of Clinical Microbiology

    Article Title: High-Throughput Real-Time Reverse Transcription-PCR Quantitation of Hepatitis C Virus RNA

    doi:

    Figure Lengend Snippet: Mean values of results (expressed as log 10 HCV RNA copies per milliliter of serum) determined by our RT-PCR protocol (ABI Prism 7700 assay), the bDNA assay and the NGI method are comparable. (A) Comparison of totals; differences in mean values are not significant (ns). The percentages of samples positive by each method are given in the box. (B) Comparison of mean values for groups of different genotypes; differences are not significant.

    Article Snippet: Seventy-four patients were HCV RNA positive by a commercial quantitative HCV RNA test with a detection limit of 102 copies/ml (Amplicor HCV-RNA; Roche Molecular Systems) and five were negative.

    Techniques: Reverse Transcription Polymerase Chain Reaction

    Schematic representation of the transcription fragment from the 5′ NCR of HCV. Oligonucleotide primers C149 and C342 were used for amplification by RT-PCR. Primers C24 and C339 were hybridized to the RNA transcript to assess its integrity. FT275 is the dual-labeled probe. R, reporter; Q, quencher; nt, nucleotides.

    Journal: Journal of Clinical Microbiology

    Article Title: High-Throughput Real-Time Reverse Transcription-PCR Quantitation of Hepatitis C Virus RNA

    doi:

    Figure Lengend Snippet: Schematic representation of the transcription fragment from the 5′ NCR of HCV. Oligonucleotide primers C149 and C342 were used for amplification by RT-PCR. Primers C24 and C339 were hybridized to the RNA transcript to assess its integrity. FT275 is the dual-labeled probe. R, reporter; Q, quencher; nt, nucleotides.

    Article Snippet: Seventy-four patients were HCV RNA positive by a commercial quantitative HCV RNA test with a detection limit of 102 copies/ml (Amplicor HCV-RNA; Roche Molecular Systems) and five were negative.

    Techniques: Amplification, Reverse Transcription Polymerase Chain Reaction, Labeling

    Standard curve of the input RNA concentration in serial dilutions of the first reference standard versus C T . Each dot represents the result of triplicate PCR amplifications for each dilution. The stars represent the results of PCR amplification of samples containing unknown quantities of HCV RNA tested by this method. R was equal to 0.999, and the slope was −3.429.

    Journal: Journal of Clinical Microbiology

    Article Title: High-Throughput Real-Time Reverse Transcription-PCR Quantitation of Hepatitis C Virus RNA

    doi:

    Figure Lengend Snippet: Standard curve of the input RNA concentration in serial dilutions of the first reference standard versus C T . Each dot represents the result of triplicate PCR amplifications for each dilution. The stars represent the results of PCR amplification of samples containing unknown quantities of HCV RNA tested by this method. R was equal to 0.999, and the slope was −3.429.

    Article Snippet: Seventy-four patients were HCV RNA positive by a commercial quantitative HCV RNA test with a detection limit of 102 copies/ml (Amplicor HCV-RNA; Roche Molecular Systems) and five were negative.

    Techniques: Concentration Assay, Polymerase Chain Reaction, Amplification

    The timeline summary of the case. HCV = hepatitis C virus, HIV = human immunodeficiency virus, RNA = ribonucleic acid. a Cobas Amplicor HCV Monitor test, v2.0, Roche Switzerland, b Abbott RealTi m e HCV assay, c Xpert HCV Viral Load.

    Journal: Medicine

    Article Title: Successful treatment of genotype 3 hepatitis C infection in a noncirrhotic HIV infected patient on chronic dialysis with the combination of sofosbuvir and velpatasvir

    doi: 10.1097/MD.0000000000013671

    Figure Lengend Snippet: The timeline summary of the case. HCV = hepatitis C virus, HIV = human immunodeficiency virus, RNA = ribonucleic acid. a Cobas Amplicor HCV Monitor test, v2.0, Roche Switzerland, b Abbott RealTi m e HCV assay, c Xpert HCV Viral Load.

    Article Snippet: His first HCV viral load was done in April 2000, the patient had 536,000 IU/mL HCV RNA (Cobas Amplicor HCV Monitor test, v2.0, Roche Switzerland).

    Techniques:

    Inhibition of HCV replication and production by IFN-α. IFN-α (100 IU/ml) was administered to the JFH1-ribozyme-transfected cells 3 days after transfection. The HCV core Ag level, HCV RNA titer, and SEAP activity in the culture supernatants

    Journal:

    Article Title: Production of Infectious Hepatitis C Virus of Various Genotypes in Cell Cultures ▿

    doi: 10.1128/JVI.02334-06

    Figure Lengend Snippet: Inhibition of HCV replication and production by IFN-α. IFN-α (100 IU/ml) was administered to the JFH1-ribozyme-transfected cells 3 days after transfection. The HCV core Ag level, HCV RNA titer, and SEAP activity in the culture supernatants

    Article Snippet: After inoculation, the chimpanzee was monitored weekly with blood samples for HCV RNA (Roche Amplicor Monitor II with a lower limit of detection of 200 copies/ml), anti-HCV (Bayer Anti-HCV EIA II), and alanine aminotransferase (ALT).

    Techniques: Inhibition, Transfection, Activity Assay

    (A) Isolation of naturally occurring HCV core particles from concentrated plasma by isopycnic centrifugation in a CsCl gradient. (B) Isolation of nucleocapsids from putative HCV virions by detergent treatment. A sample (1.5 ml) of the fraction of the gradient banding at a density of 1.10 g/ml (shown in panel A) and corresponding to the HCV-RNA peak was treated with 0.5% Tween 80 and centrifuged in a CsCl gradient as shown in panel A. Fractions (0.7 ml) were tested for HCV RNA by RT-PCR and for HCV core antigen by ELISA. O.D., optical density. Ag, antigen.

    Journal: Journal of Virology

    Article Title: Nonenveloped Nucleocapsids of Hepatitis C Virus in the Serum of Infected Patients

    doi: 10.1128/JVI.75.17.8240-8250.2001

    Figure Lengend Snippet: (A) Isolation of naturally occurring HCV core particles from concentrated plasma by isopycnic centrifugation in a CsCl gradient. (B) Isolation of nucleocapsids from putative HCV virions by detergent treatment. A sample (1.5 ml) of the fraction of the gradient banding at a density of 1.10 g/ml (shown in panel A) and corresponding to the HCV-RNA peak was treated with 0.5% Tween 80 and centrifuged in a CsCl gradient as shown in panel A. Fractions (0.7 ml) were tested for HCV RNA by RT-PCR and for HCV core antigen by ELISA. O.D., optical density. Ag, antigen.

    Article Snippet: Both chimpanzees tested positive for HCV RNA (5.8 log IU/ml in CH1537 and 5.7 log IU/ml in CH 1572 by Amplicor monitor; Roche).

    Techniques: Isolation, Centrifugation, Reverse Transcription Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay