Amplicor Hcv Rna, supplied by Roche, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "High-Throughput Real-Time Reverse Transcription-PCR Quantitation of Hepatitis C Virus RNA"
Article Title: High-Throughput Real-Time Reverse Transcription-PCR Quantitation of Hepatitis C Virus RNA
Journal: Journal of Clinical Microbiology
Figure Legend Snippet: Mean values of results (expressed as log 10 HCV RNA copies per milliliter of serum) determined by our RT-PCR protocol (ABI Prism 7700 assay), the bDNA assay and the NGI method are comparable. (A) Comparison of totals; differences in mean values are not significant (ns). The percentages of samples positive by each method are given in the box. (B) Comparison of mean values for groups of different genotypes; differences are not significant.
Techniques Used: Reverse Transcription Polymerase Chain Reaction
Figure Legend Snippet: Schematic representation of the transcription fragment from the 5′ NCR of HCV. Oligonucleotide primers C149 and C342 were used for amplification by RT-PCR. Primers C24 and C339 were hybridized to the RNA transcript to assess its integrity. FT275 is the dual-labeled probe. R, reporter; Q, quencher; nt, nucleotides.
Techniques Used: Amplification, Reverse Transcription Polymerase Chain Reaction, Labeling
Figure Legend Snippet: Standard curve of the input RNA concentration in serial dilutions of the first reference standard versus C T . Each dot represents the result of triplicate PCR amplifications for each dilution. The stars represent the results of PCR amplification of samples containing unknown quantities of HCV RNA tested by this method. R was equal to 0.999, and the slope was −3.429.
Techniques Used: Concentration Assay, Polymerase Chain Reaction, Amplification