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Mirus Bio amplicons
Generation of the ChIP <t>amplicons</t> for ChIP on chip experiments. ( A ) ChIP of HaCaT cells with α-p63 and control α-Flag antibodies. The known targets tested are indicated. Numbers on the left represent the PCR cycles. ( B ) Size distribution of the amplicons generated after 45 cycles of PCR. Molecular weight markers are indicated on the left lane. ( C ) Control of relative enrichment of the indicated amplicons with the same genomic targets tested in (A).
Amplicons, supplied by Mirus Bio, used in various techniques. Bioz Stars score: 92/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Images

1) Product Images from "New p63 targets in keratinocytes identified by a genome-wide approach"

Article Title: New p63 targets in keratinocytes identified by a genome-wide approach

Journal: The EMBO Journal

doi: 10.1038/sj.emboj.7601375

Generation of the ChIP amplicons for ChIP on chip experiments. ( A ) ChIP of HaCaT cells with α-p63 and control α-Flag antibodies. The known targets tested are indicated. Numbers on the left represent the PCR cycles. ( B ) Size distribution of the amplicons generated after 45 cycles of PCR. Molecular weight markers are indicated on the left lane. ( C ) Control of relative enrichment of the indicated amplicons with the same genomic targets tested in (A).
Figure Legend Snippet: Generation of the ChIP amplicons for ChIP on chip experiments. ( A ) ChIP of HaCaT cells with α-p63 and control α-Flag antibodies. The known targets tested are indicated. Numbers on the left represent the PCR cycles. ( B ) Size distribution of the amplicons generated after 45 cycles of PCR. Molecular weight markers are indicated on the left lane. ( C ) Control of relative enrichment of the indicated amplicons with the same genomic targets tested in (A).

Techniques Used: Chromatin Immunoprecipitation, Polymerase Chain Reaction, Generated, Molecular Weight

2) Product Images from "Molecular detection of harmful algal blooms (HABs) using locked nucleic acids and bead array technology"

Article Title: Molecular detection of harmful algal blooms (HABs) using locked nucleic acids and bead array technology

Journal: Limnology and oceanography, methods / ASLO

doi: 10.4319/lom.2010.8.269

Analytic detection limits of chemically and enzymatically labeled amplicons. The amplicons were serially diluted and hybridized for 1.5 h at 53°C. The error bars represent the standard deviation. The samples were run in duplicate, and the experiments
Figure Legend Snippet: Analytic detection limits of chemically and enzymatically labeled amplicons. The amplicons were serially diluted and hybridized for 1.5 h at 53°C. The error bars represent the standard deviation. The samples were run in duplicate, and the experiments

Techniques Used: Labeling, Standard Deviation

Performance of species-specific probes with field samples. Hybridization assay conditions used 15 μL chemical-labeled IT amplicon. The amplicons were generated using 40 cycles, and amplification was done with the universal primer set D2C and DIRF.
Figure Legend Snippet: Performance of species-specific probes with field samples. Hybridization assay conditions used 15 μL chemical-labeled IT amplicon. The amplicons were generated using 40 cycles, and amplification was done with the universal primer set D2C and DIRF.

Techniques Used: Hybridization, Labeling, Amplification, Generated

Comparison of Mirus Label IT and 5′ end labeled amplicons. Fifty nanograms of the labeled template was hybridized with different sets of K. brevis (Pkb, Pkb2, PKB658, Pkb2lna, PKB658lna) and K. mikimotoi probes (Pkmiklna and Pkmiklna2). Hybridization
Figure Legend Snippet: Comparison of Mirus Label IT and 5′ end labeled amplicons. Fifty nanograms of the labeled template was hybridized with different sets of K. brevis (Pkb, Pkb2, PKB658, Pkb2lna, PKB658lna) and K. mikimotoi probes (Pkmiklna and Pkmiklna2). Hybridization

Techniques Used: Labeling, Hybridization

Specificity of probes with Mirus Label IT D1/D2 amplicons. Probes were challenged with closely and non–closely related algal species. The hybridization was carried out for 1.5 h at 53°C and used 33 ng amplicon per well. The samples were
Figure Legend Snippet: Specificity of probes with Mirus Label IT D1/D2 amplicons. Probes were challenged with closely and non–closely related algal species. The hybridization was carried out for 1.5 h at 53°C and used 33 ng amplicon per well. The samples were

Techniques Used: Hybridization, Amplification

3) Product Images from "Molecular detection of harmful algal blooms (HABs) using locked nucleic acids and bead array technology"

Article Title: Molecular detection of harmful algal blooms (HABs) using locked nucleic acids and bead array technology

Journal: Limnology and oceanography, methods / ASLO

doi: 10.4319/lom.2010.8.269

Analytic detection limits of chemically and enzymatically labeled amplicons. The amplicons were serially diluted and hybridized for 1.5 h at 53°C. The error bars represent the standard deviation. The samples were run in duplicate, and the experiments
Figure Legend Snippet: Analytic detection limits of chemically and enzymatically labeled amplicons. The amplicons were serially diluted and hybridized for 1.5 h at 53°C. The error bars represent the standard deviation. The samples were run in duplicate, and the experiments

Techniques Used: Labeling, Standard Deviation

Performance of species-specific probes with field samples. Hybridization assay conditions used 15 μL chemical-labeled IT amplicon. The amplicons were generated using 40 cycles, and amplification was done with the universal primer set D2C and DIRF.
Figure Legend Snippet: Performance of species-specific probes with field samples. Hybridization assay conditions used 15 μL chemical-labeled IT amplicon. The amplicons were generated using 40 cycles, and amplification was done with the universal primer set D2C and DIRF.

Techniques Used: Hybridization, Labeling, Amplification, Generated

Comparison of Mirus Label IT and 5′ end labeled amplicons. Fifty nanograms of the labeled template was hybridized with different sets of K. brevis (Pkb, Pkb2, PKB658, Pkb2lna, PKB658lna) and K. mikimotoi probes (Pkmiklna and Pkmiklna2). Hybridization
Figure Legend Snippet: Comparison of Mirus Label IT and 5′ end labeled amplicons. Fifty nanograms of the labeled template was hybridized with different sets of K. brevis (Pkb, Pkb2, PKB658, Pkb2lna, PKB658lna) and K. mikimotoi probes (Pkmiklna and Pkmiklna2). Hybridization

Techniques Used: Labeling, Hybridization

Specificity of probes with Mirus Label IT D1/D2 amplicons. Probes were challenged with closely and non–closely related algal species. The hybridization was carried out for 1.5 h at 53°C and used 33 ng amplicon per well. The samples were
Figure Legend Snippet: Specificity of probes with Mirus Label IT D1/D2 amplicons. Probes were challenged with closely and non–closely related algal species. The hybridization was carried out for 1.5 h at 53°C and used 33 ng amplicon per well. The samples were

Techniques Used: Hybridization, Amplification

4) Product Images from "Molecular detection of harmful algal blooms (HABs) using locked nucleic acids and bead array technology"

Article Title: Molecular detection of harmful algal blooms (HABs) using locked nucleic acids and bead array technology

Journal: Limnology and oceanography, methods / ASLO

doi: 10.4319/lom.2010.8.269

Analytic detection limits of chemically and enzymatically labeled amplicons. The amplicons were serially diluted and hybridized for 1.5 h at 53°C. The error bars represent the standard deviation. The samples were run in duplicate, and the experiments
Figure Legend Snippet: Analytic detection limits of chemically and enzymatically labeled amplicons. The amplicons were serially diluted and hybridized for 1.5 h at 53°C. The error bars represent the standard deviation. The samples were run in duplicate, and the experiments

Techniques Used: Labeling, Standard Deviation

Performance of species-specific probes with field samples. Hybridization assay conditions used 15 μL chemical-labeled IT amplicon. The amplicons were generated using 40 cycles, and amplification was done with the universal primer set D2C and DIRF.
Figure Legend Snippet: Performance of species-specific probes with field samples. Hybridization assay conditions used 15 μL chemical-labeled IT amplicon. The amplicons were generated using 40 cycles, and amplification was done with the universal primer set D2C and DIRF.

Techniques Used: Hybridization, Labeling, Amplification, Generated

Comparison of Mirus Label IT and 5′ end labeled amplicons. Fifty nanograms of the labeled template was hybridized with different sets of K. brevis (Pkb, Pkb2, PKB658, Pkb2lna, PKB658lna) and K. mikimotoi probes (Pkmiklna and Pkmiklna2). Hybridization
Figure Legend Snippet: Comparison of Mirus Label IT and 5′ end labeled amplicons. Fifty nanograms of the labeled template was hybridized with different sets of K. brevis (Pkb, Pkb2, PKB658, Pkb2lna, PKB658lna) and K. mikimotoi probes (Pkmiklna and Pkmiklna2). Hybridization

Techniques Used: Labeling, Hybridization

Specificity of probes with Mirus Label IT D1/D2 amplicons. Probes were challenged with closely and non–closely related algal species. The hybridization was carried out for 1.5 h at 53°C and used 33 ng amplicon per well. The samples were
Figure Legend Snippet: Specificity of probes with Mirus Label IT D1/D2 amplicons. Probes were challenged with closely and non–closely related algal species. The hybridization was carried out for 1.5 h at 53°C and used 33 ng amplicon per well. The samples were

Techniques Used: Hybridization, Amplification

5) Product Images from "Molecular detection of harmful algal blooms (HABs) using locked nucleic acids and bead array technology"

Article Title: Molecular detection of harmful algal blooms (HABs) using locked nucleic acids and bead array technology

Journal: Limnology and oceanography, methods / ASLO

doi: 10.4319/lom.2010.8.269

Analytic detection limits of chemically and enzymatically labeled amplicons. The amplicons were serially diluted and hybridized for 1.5 h at 53°C. The error bars represent the standard deviation. The samples were run in duplicate, and the experiments
Figure Legend Snippet: Analytic detection limits of chemically and enzymatically labeled amplicons. The amplicons were serially diluted and hybridized for 1.5 h at 53°C. The error bars represent the standard deviation. The samples were run in duplicate, and the experiments

Techniques Used: Labeling, Standard Deviation

Performance of species-specific probes with field samples. Hybridization assay conditions used 15 μL chemical-labeled IT amplicon. The amplicons were generated using 40 cycles, and amplification was done with the universal primer set D2C and DIRF.
Figure Legend Snippet: Performance of species-specific probes with field samples. Hybridization assay conditions used 15 μL chemical-labeled IT amplicon. The amplicons were generated using 40 cycles, and amplification was done with the universal primer set D2C and DIRF.

Techniques Used: Hybridization, Labeling, Amplification, Generated

Comparison of Mirus Label IT and 5′ end labeled amplicons. Fifty nanograms of the labeled template was hybridized with different sets of K. brevis (Pkb, Pkb2, PKB658, Pkb2lna, PKB658lna) and K. mikimotoi probes (Pkmiklna and Pkmiklna2). Hybridization
Figure Legend Snippet: Comparison of Mirus Label IT and 5′ end labeled amplicons. Fifty nanograms of the labeled template was hybridized with different sets of K. brevis (Pkb, Pkb2, PKB658, Pkb2lna, PKB658lna) and K. mikimotoi probes (Pkmiklna and Pkmiklna2). Hybridization

Techniques Used: Labeling, Hybridization

Specificity of probes with Mirus Label IT D1/D2 amplicons. Probes were challenged with closely and non–closely related algal species. The hybridization was carried out for 1.5 h at 53°C and used 33 ng amplicon per well. The samples were
Figure Legend Snippet: Specificity of probes with Mirus Label IT D1/D2 amplicons. Probes were challenged with closely and non–closely related algal species. The hybridization was carried out for 1.5 h at 53°C and used 33 ng amplicon per well. The samples were

Techniques Used: Hybridization, Amplification

6) Product Images from "C/EBP? Gene Targets in Human Keratinocytes"

Article Title: C/EBP? Gene Targets in Human Keratinocytes

Journal: PLoS ONE

doi: 10.1371/journal.pone.0013789

Identification of C/EBPδ targets by ChIP on chip. A. Generation of the ChIP amplicons for ChIP on chip experiments. ChIP of human primary keratinocytes with C/EBPδ and control antibodies. The known targets tested are indicated. The normalization of the immunoprecipitated material was performed by evaluating a negative genomic region (Centromeric Satellite 11). B. Gene Ontology analysis of C/EBPδ targets genes from the ChIP on chip screening.
Figure Legend Snippet: Identification of C/EBPδ targets by ChIP on chip. A. Generation of the ChIP amplicons for ChIP on chip experiments. ChIP of human primary keratinocytes with C/EBPδ and control antibodies. The known targets tested are indicated. The normalization of the immunoprecipitated material was performed by evaluating a negative genomic region (Centromeric Satellite 11). B. Gene Ontology analysis of C/EBPδ targets genes from the ChIP on chip screening.

Techniques Used: Chromatin Immunoprecipitation, Immunoprecipitation

7) Product Images from "Waterborne Pathogen Detection by Use of Oligonucleotide-Based Microarrays"

Article Title: Waterborne Pathogen Detection by Use of Oligonucleotide-Based Microarrays

Journal: Applied and Environmental Microbiology

doi: 10.1128/AEM.71.12.8548-8557.2005

Hybridization of 16S rRNA fragments amplified from purified genomic S. enterica serovar Typhimurium, E. coli , and Y. enterocolitica DNAs on the prototype microarray. Hybridization of 25 ng of 16S rRNA amplicons from genomic DNAs of E. coli , S. enterica serovar Typhimurium, and Y. enterocolitica is shown. Results are shown as the logarithm (base 2) ratio of the probe's fluorescence intensity relative to control (buffer) spots, after normalization. The error bars represent standard errors, and each result represents the average of six spot intensities derived from two different microarray hybridizations.
Figure Legend Snippet: Hybridization of 16S rRNA fragments amplified from purified genomic S. enterica serovar Typhimurium, E. coli , and Y. enterocolitica DNAs on the prototype microarray. Hybridization of 25 ng of 16S rRNA amplicons from genomic DNAs of E. coli , S. enterica serovar Typhimurium, and Y. enterocolitica is shown. Results are shown as the logarithm (base 2) ratio of the probe's fluorescence intensity relative to control (buffer) spots, after normalization. The error bars represent standard errors, and each result represents the average of six spot intensities derived from two different microarray hybridizations.

Techniques Used: Hybridization, Amplification, Purification, Microarray, Fluorescence, Derivative Assay

Hybridization of 16S rRNA, cpn60 , or wecE amplicons generated from different ratios of S. enterica serovar Typhimurium DNA in wastewater DNA. Variable amounts of S. enterica serovar Typhimurium DNA were added to wastewater DNA (0%, 0.1%, 1.0%, and 5.0% final concentrations). The mixtures were subjected to PCR using universal 16S rRNA (A), cpn60 (B), or wecE (C) primers. One microgram of each amplicon was labeled and separately hybridized to the prototype microarray. Results are shown as the logarithm (base 2) ratio of the probe's fluorescence intensity relative to control (buffer) spots, after normalization. The error bars represent standard errors, and each result represents the average of six spot intensities derived from two different microarray hybridizations.
Figure Legend Snippet: Hybridization of 16S rRNA, cpn60 , or wecE amplicons generated from different ratios of S. enterica serovar Typhimurium DNA in wastewater DNA. Variable amounts of S. enterica serovar Typhimurium DNA were added to wastewater DNA (0%, 0.1%, 1.0%, and 5.0% final concentrations). The mixtures were subjected to PCR using universal 16S rRNA (A), cpn60 (B), or wecE (C) primers. One microgram of each amplicon was labeled and separately hybridized to the prototype microarray. Results are shown as the logarithm (base 2) ratio of the probe's fluorescence intensity relative to control (buffer) spots, after normalization. The error bars represent standard errors, and each result represents the average of six spot intensities derived from two different microarray hybridizations.

Techniques Used: Hybridization, Generated, Polymerase Chain Reaction, Amplification, Labeling, Microarray, Fluorescence, Derivative Assay

8) Product Images from "Molecular detection of harmful algal blooms (HABs) using locked nucleic acids and bead array technology"

Article Title: Molecular detection of harmful algal blooms (HABs) using locked nucleic acids and bead array technology

Journal: Limnology and oceanography, methods / ASLO

doi: 10.4319/lom.2010.8.269

Analytic detection limits of chemically and enzymatically labeled amplicons. The amplicons were serially diluted and hybridized for 1.5 h at 53°C. The error bars represent the standard deviation. The samples were run in duplicate, and the experiments
Figure Legend Snippet: Analytic detection limits of chemically and enzymatically labeled amplicons. The amplicons were serially diluted and hybridized for 1.5 h at 53°C. The error bars represent the standard deviation. The samples were run in duplicate, and the experiments

Techniques Used: Labeling, Standard Deviation

Performance of species-specific probes with field samples. Hybridization assay conditions used 15 μL chemical-labeled IT amplicon. The amplicons were generated using 40 cycles, and amplification was done with the universal primer set D2C and DIRF.
Figure Legend Snippet: Performance of species-specific probes with field samples. Hybridization assay conditions used 15 μL chemical-labeled IT amplicon. The amplicons were generated using 40 cycles, and amplification was done with the universal primer set D2C and DIRF.

Techniques Used: Hybridization, Labeling, Amplification, Generated

Comparison of Mirus Label IT and 5′ end labeled amplicons. Fifty nanograms of the labeled template was hybridized with different sets of K. brevis (Pkb, Pkb2, PKB658, Pkb2lna, PKB658lna) and K. mikimotoi probes (Pkmiklna and Pkmiklna2). Hybridization
Figure Legend Snippet: Comparison of Mirus Label IT and 5′ end labeled amplicons. Fifty nanograms of the labeled template was hybridized with different sets of K. brevis (Pkb, Pkb2, PKB658, Pkb2lna, PKB658lna) and K. mikimotoi probes (Pkmiklna and Pkmiklna2). Hybridization

Techniques Used: Labeling, Hybridization

Specificity of probes with Mirus Label IT D1/D2 amplicons. Probes were challenged with closely and non–closely related algal species. The hybridization was carried out for 1.5 h at 53°C and used 33 ng amplicon per well. The samples were
Figure Legend Snippet: Specificity of probes with Mirus Label IT D1/D2 amplicons. Probes were challenged with closely and non–closely related algal species. The hybridization was carried out for 1.5 h at 53°C and used 33 ng amplicon per well. The samples were

Techniques Used: Hybridization, Amplification

9) Product Images from "Molecular detection of harmful algal blooms (HABs) using locked nucleic acids and bead array technology"

Article Title: Molecular detection of harmful algal blooms (HABs) using locked nucleic acids and bead array technology

Journal: Limnology and oceanography, methods / ASLO

doi: 10.4319/lom.2010.8.269

Analytic detection limits of chemically and enzymatically labeled amplicons. The amplicons were serially diluted and hybridized for 1.5 h at 53°C. The error bars represent the standard deviation. The samples were run in duplicate, and the experiments
Figure Legend Snippet: Analytic detection limits of chemically and enzymatically labeled amplicons. The amplicons were serially diluted and hybridized for 1.5 h at 53°C. The error bars represent the standard deviation. The samples were run in duplicate, and the experiments

Techniques Used: Labeling, Standard Deviation

Performance of species-specific probes with field samples. Hybridization assay conditions used 15 μL chemical-labeled IT amplicon. The amplicons were generated using 40 cycles, and amplification was done with the universal primer set D2C and DIRF.
Figure Legend Snippet: Performance of species-specific probes with field samples. Hybridization assay conditions used 15 μL chemical-labeled IT amplicon. The amplicons were generated using 40 cycles, and amplification was done with the universal primer set D2C and DIRF.

Techniques Used: Hybridization, Labeling, Amplification, Generated

Comparison of Mirus Label IT and 5′ end labeled amplicons. Fifty nanograms of the labeled template was hybridized with different sets of K. brevis (Pkb, Pkb2, PKB658, Pkb2lna, PKB658lna) and K. mikimotoi probes (Pkmiklna and Pkmiklna2). Hybridization
Figure Legend Snippet: Comparison of Mirus Label IT and 5′ end labeled amplicons. Fifty nanograms of the labeled template was hybridized with different sets of K. brevis (Pkb, Pkb2, PKB658, Pkb2lna, PKB658lna) and K. mikimotoi probes (Pkmiklna and Pkmiklna2). Hybridization

Techniques Used: Labeling, Hybridization

Specificity of probes with Mirus Label IT D1/D2 amplicons. Probes were challenged with closely and non–closely related algal species. The hybridization was carried out for 1.5 h at 53°C and used 33 ng amplicon per well. The samples were
Figure Legend Snippet: Specificity of probes with Mirus Label IT D1/D2 amplicons. Probes were challenged with closely and non–closely related algal species. The hybridization was carried out for 1.5 h at 53°C and used 33 ng amplicon per well. The samples were

Techniques Used: Hybridization, Amplification

10) Product Images from "Molecular detection of harmful algal blooms (HABs) using locked nucleic acids and bead array technology"

Article Title: Molecular detection of harmful algal blooms (HABs) using locked nucleic acids and bead array technology

Journal: Limnology and oceanography, methods / ASLO

doi: 10.4319/lom.2010.8.269

Analytic detection limits of chemically and enzymatically labeled amplicons. The amplicons were serially diluted and hybridized for 1.5 h at 53°C. The error bars represent the standard deviation. The samples were run in duplicate, and the experiments
Figure Legend Snippet: Analytic detection limits of chemically and enzymatically labeled amplicons. The amplicons were serially diluted and hybridized for 1.5 h at 53°C. The error bars represent the standard deviation. The samples were run in duplicate, and the experiments

Techniques Used: Labeling, Standard Deviation

Performance of species-specific probes with field samples. Hybridization assay conditions used 15 μL chemical-labeled IT amplicon. The amplicons were generated using 40 cycles, and amplification was done with the universal primer set D2C and DIRF.
Figure Legend Snippet: Performance of species-specific probes with field samples. Hybridization assay conditions used 15 μL chemical-labeled IT amplicon. The amplicons were generated using 40 cycles, and amplification was done with the universal primer set D2C and DIRF.

Techniques Used: Hybridization, Labeling, Amplification, Generated

Comparison of Mirus Label IT and 5′ end labeled amplicons. Fifty nanograms of the labeled template was hybridized with different sets of K. brevis (Pkb, Pkb2, PKB658, Pkb2lna, PKB658lna) and K. mikimotoi probes (Pkmiklna and Pkmiklna2). Hybridization
Figure Legend Snippet: Comparison of Mirus Label IT and 5′ end labeled amplicons. Fifty nanograms of the labeled template was hybridized with different sets of K. brevis (Pkb, Pkb2, PKB658, Pkb2lna, PKB658lna) and K. mikimotoi probes (Pkmiklna and Pkmiklna2). Hybridization

Techniques Used: Labeling, Hybridization

Specificity of probes with Mirus Label IT D1/D2 amplicons. Probes were challenged with closely and non–closely related algal species. The hybridization was carried out for 1.5 h at 53°C and used 33 ng amplicon per well. The samples were
Figure Legend Snippet: Specificity of probes with Mirus Label IT D1/D2 amplicons. Probes were challenged with closely and non–closely related algal species. The hybridization was carried out for 1.5 h at 53°C and used 33 ng amplicon per well. The samples were

Techniques Used: Hybridization, Amplification

11) Product Images from "Molecular detection of harmful algal blooms (HABs) using locked nucleic acids and bead array technology"

Article Title: Molecular detection of harmful algal blooms (HABs) using locked nucleic acids and bead array technology

Journal: Limnology and oceanography, methods / ASLO

doi: 10.4319/lom.2010.8.269

Analytic detection limits of chemically and enzymatically labeled amplicons. The amplicons were serially diluted and hybridized for 1.5 h at 53°C. The error bars represent the standard deviation. The samples were run in duplicate, and the experiments
Figure Legend Snippet: Analytic detection limits of chemically and enzymatically labeled amplicons. The amplicons were serially diluted and hybridized for 1.5 h at 53°C. The error bars represent the standard deviation. The samples were run in duplicate, and the experiments

Techniques Used: Labeling, Standard Deviation

Performance of species-specific probes with field samples. Hybridization assay conditions used 15 μL chemical-labeled IT amplicon. The amplicons were generated using 40 cycles, and amplification was done with the universal primer set D2C and DIRF.
Figure Legend Snippet: Performance of species-specific probes with field samples. Hybridization assay conditions used 15 μL chemical-labeled IT amplicon. The amplicons were generated using 40 cycles, and amplification was done with the universal primer set D2C and DIRF.

Techniques Used: Hybridization, Labeling, Amplification, Generated

Comparison of Mirus Label IT and 5′ end labeled amplicons. Fifty nanograms of the labeled template was hybridized with different sets of K. brevis (Pkb, Pkb2, PKB658, Pkb2lna, PKB658lna) and K. mikimotoi probes (Pkmiklna and Pkmiklna2). Hybridization
Figure Legend Snippet: Comparison of Mirus Label IT and 5′ end labeled amplicons. Fifty nanograms of the labeled template was hybridized with different sets of K. brevis (Pkb, Pkb2, PKB658, Pkb2lna, PKB658lna) and K. mikimotoi probes (Pkmiklna and Pkmiklna2). Hybridization

Techniques Used: Labeling, Hybridization

Specificity of probes with Mirus Label IT D1/D2 amplicons. Probes were challenged with closely and non–closely related algal species. The hybridization was carried out for 1.5 h at 53°C and used 33 ng amplicon per well. The samples were
Figure Legend Snippet: Specificity of probes with Mirus Label IT D1/D2 amplicons. Probes were challenged with closely and non–closely related algal species. The hybridization was carried out for 1.5 h at 53°C and used 33 ng amplicon per well. The samples were

Techniques Used: Hybridization, Amplification

Related Articles

Polymerase Chain Reaction:

Article Title: Molecular detection of harmful algal blooms (HABs) using locked nucleic acids and bead array technology
Article Snippet: .. At that temperature, nonmodified probes Pkb658 and Pkb2 displayed fluorescent signal reductions as high as 72% when tested with Mirus Label IT amplicons ( ) and up to 81% with PCR biotinylated amplicons (data not shown). ..

Article Title: C/EBP? Gene Targets in Human Keratinocytes
Article Snippet: .. 5 µg of amplicons for Flag and input DNA (subjected to the same number of PCR manipulations) were labeled using the LabelIT Cy5/Cy3 Nucleic acid labeling kit (Mirus), following the manufacturer's instructions, with a reagent to DNA ratio of 2.5 for Cy5 (IPs) and 1.5 for Cy3 (Input). .. The CpG 21K slides were purchased from University Health Network, Toronto, Canada.

Article Title: New p63 targets in keratinocytes identified by a genome-wide approach
Article Snippet: .. Five micrograms of amplicons for α-p63, α-Flag and input DNA (subjected to the same number of PCR cycles) were labelled using the LabelIT Cy5/Cy3 Nucleic acid labelling kit (Mirus), following the manufacturer's instructions, with a reagent to DNA ratio of 2.5 for Cy5 (IPs) and 1.5 for Cy3 (input). ..

Fluorescence:

Article Title: Molecular detection of harmful algal blooms (HABs) using locked nucleic acids and bead array technology
Article Snippet: .. The results showed that incorporation of LNA residues to probe sequences did not significantly enhance the fluorescence signal intensity when the assay was undertaken at 50°C and used Mirus Label IT amplicons ( ). .. However, a moderate enhancement in fluorescence signal intensity ranging from 11.5% to 33%, was documented for Pkmikilna and Pkb2lna, when these probes were challenged with enzymatically labeled amplicons (data not shown).

Labeling:

Article Title: C/EBP? Gene Targets in Human Keratinocytes
Article Snippet: .. 5 µg of amplicons for Flag and input DNA (subjected to the same number of PCR manipulations) were labeled using the LabelIT Cy5/Cy3 Nucleic acid labeling kit (Mirus), following the manufacturer's instructions, with a reagent to DNA ratio of 2.5 for Cy5 (IPs) and 1.5 for Cy3 (Input). .. The CpG 21K slides were purchased from University Health Network, Toronto, Canada.

Article Title: Molecular detection of harmful algal blooms (HABs) using locked nucleic acids and bead array technology
Article Snippet: .. After purification, the amplicons were biotin labeled with the Mirus Label IT kit (Mirus, Madison, WI). .. The labeling reactions were incubated in the dark at 37°C for 3 h and used a final volume of 20 μL and a 1:2 ratio of nucleic acid to labeling reagent.

Article Title: Molecular detection of harmful algal blooms (HABs) using locked nucleic acids and bead array technology
Article Snippet: .. All eight probes showed significantly higher signal fluorescent intensities when challenged with amplicons labeled with Mirus Label IT technique ( ). ..

Article Title: Waterborne Pathogen Detection by Use of Oligonucleotide-Based Microarrays
Article Snippet: .. Two micrograms of purified amplicons was chemically labeled with a Mirus Cy5 Label IT nucleic acid labeling kit (Mirus, Madison, Wis.) according to the manufacturer's instructions. .. As the labeling reaction was performed in a small volume (30 μl), a quick spin was performed after 30min of incubation to minimize evaporation loss.

Article Title: Molecular detection of harmful algal blooms (HABs) using locked nucleic acids and bead array technology
Article Snippet: .. Chemically labeled amplicons (Mirus Label IT amplicons): For chemically labeled amplicons, illustrates the detection levels of the assay conducted at 53°C. .. A steady decrease in fluorescence signal was documented as the amount of Mirus Label IT amplicon was decreased from 50 ng to 500 pg.

Purification:

Article Title: Molecular detection of harmful algal blooms (HABs) using locked nucleic acids and bead array technology
Article Snippet: .. After purification, the amplicons were biotin labeled with the Mirus Label IT kit (Mirus, Madison, WI). .. The labeling reactions were incubated in the dark at 37°C for 3 h and used a final volume of 20 μL and a 1:2 ratio of nucleic acid to labeling reagent.

Article Title: Waterborne Pathogen Detection by Use of Oligonucleotide-Based Microarrays
Article Snippet: .. Two micrograms of purified amplicons was chemically labeled with a Mirus Cy5 Label IT nucleic acid labeling kit (Mirus, Madison, Wis.) according to the manufacturer's instructions. .. As the labeling reaction was performed in a small volume (30 μl), a quick spin was performed after 30min of incubation to minimize evaporation loss.

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    Mirus Bio amplicons
    Generation of the ChIP <t>amplicons</t> for ChIP on chip experiments. ( A ) ChIP of HaCaT cells with α-p63 and control α-Flag antibodies. The known targets tested are indicated. Numbers on the left represent the PCR cycles. ( B ) Size distribution of the amplicons generated after 45 cycles of PCR. Molecular weight markers are indicated on the left lane. ( C ) Control of relative enrichment of the indicated amplicons with the same genomic targets tested in (A).
    Amplicons, supplied by Mirus Bio, used in various techniques. Bioz Stars score: 92/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/amplicons/product/Mirus Bio
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    Generation of the ChIP amplicons for ChIP on chip experiments. ( A ) ChIP of HaCaT cells with α-p63 and control α-Flag antibodies. The known targets tested are indicated. Numbers on the left represent the PCR cycles. ( B ) Size distribution of the amplicons generated after 45 cycles of PCR. Molecular weight markers are indicated on the left lane. ( C ) Control of relative enrichment of the indicated amplicons with the same genomic targets tested in (A).

    Journal: The EMBO Journal

    Article Title: New p63 targets in keratinocytes identified by a genome-wide approach

    doi: 10.1038/sj.emboj.7601375

    Figure Lengend Snippet: Generation of the ChIP amplicons for ChIP on chip experiments. ( A ) ChIP of HaCaT cells with α-p63 and control α-Flag antibodies. The known targets tested are indicated. Numbers on the left represent the PCR cycles. ( B ) Size distribution of the amplicons generated after 45 cycles of PCR. Molecular weight markers are indicated on the left lane. ( C ) Control of relative enrichment of the indicated amplicons with the same genomic targets tested in (A).

    Article Snippet: Five micrograms of amplicons for α-p63, α-Flag and input DNA (subjected to the same number of PCR cycles) were labelled using the LabelIT Cy5/Cy3 Nucleic acid labelling kit (Mirus), following the manufacturer's instructions, with a reagent to DNA ratio of 2.5 for Cy5 (IPs) and 1.5 for Cy3 (input).

    Techniques: Chromatin Immunoprecipitation, Polymerase Chain Reaction, Generated, Molecular Weight

    Analytic detection limits of chemically and enzymatically labeled amplicons. The amplicons were serially diluted and hybridized for 1.5 h at 53°C. The error bars represent the standard deviation. The samples were run in duplicate, and the experiments

    Journal: Limnology and oceanography, methods / ASLO

    Article Title: Molecular detection of harmful algal blooms (HABs) using locked nucleic acids and bead array technology

    doi: 10.4319/lom.2010.8.269

    Figure Lengend Snippet: Analytic detection limits of chemically and enzymatically labeled amplicons. The amplicons were serially diluted and hybridized for 1.5 h at 53°C. The error bars represent the standard deviation. The samples were run in duplicate, and the experiments

    Article Snippet: After purification, the amplicons were biotin labeled with the Mirus Label IT kit (Mirus, Madison, WI).

    Techniques: Labeling, Standard Deviation

    Performance of species-specific probes with field samples. Hybridization assay conditions used 15 μL chemical-labeled IT amplicon. The amplicons were generated using 40 cycles, and amplification was done with the universal primer set D2C and DIRF.

    Journal: Limnology and oceanography, methods / ASLO

    Article Title: Molecular detection of harmful algal blooms (HABs) using locked nucleic acids and bead array technology

    doi: 10.4319/lom.2010.8.269

    Figure Lengend Snippet: Performance of species-specific probes with field samples. Hybridization assay conditions used 15 μL chemical-labeled IT amplicon. The amplicons were generated using 40 cycles, and amplification was done with the universal primer set D2C and DIRF.

    Article Snippet: After purification, the amplicons were biotin labeled with the Mirus Label IT kit (Mirus, Madison, WI).

    Techniques: Hybridization, Labeling, Amplification, Generated

    Comparison of Mirus Label IT and 5′ end labeled amplicons. Fifty nanograms of the labeled template was hybridized with different sets of K. brevis (Pkb, Pkb2, PKB658, Pkb2lna, PKB658lna) and K. mikimotoi probes (Pkmiklna and Pkmiklna2). Hybridization

    Journal: Limnology and oceanography, methods / ASLO

    Article Title: Molecular detection of harmful algal blooms (HABs) using locked nucleic acids and bead array technology

    doi: 10.4319/lom.2010.8.269

    Figure Lengend Snippet: Comparison of Mirus Label IT and 5′ end labeled amplicons. Fifty nanograms of the labeled template was hybridized with different sets of K. brevis (Pkb, Pkb2, PKB658, Pkb2lna, PKB658lna) and K. mikimotoi probes (Pkmiklna and Pkmiklna2). Hybridization

    Article Snippet: After purification, the amplicons were biotin labeled with the Mirus Label IT kit (Mirus, Madison, WI).

    Techniques: Labeling, Hybridization

    Specificity of probes with Mirus Label IT D1/D2 amplicons. Probes were challenged with closely and non–closely related algal species. The hybridization was carried out for 1.5 h at 53°C and used 33 ng amplicon per well. The samples were

    Journal: Limnology and oceanography, methods / ASLO

    Article Title: Molecular detection of harmful algal blooms (HABs) using locked nucleic acids and bead array technology

    doi: 10.4319/lom.2010.8.269

    Figure Lengend Snippet: Specificity of probes with Mirus Label IT D1/D2 amplicons. Probes were challenged with closely and non–closely related algal species. The hybridization was carried out for 1.5 h at 53°C and used 33 ng amplicon per well. The samples were

    Article Snippet: After purification, the amplicons were biotin labeled with the Mirus Label IT kit (Mirus, Madison, WI).

    Techniques: Hybridization, Amplification