Structured Review

LGC Genomics GmbH amplicons
Representative image of an agarose gel electrophoresis for the <t>amplicons</t> COI-lf and 16S-sf ( 1 = A. brevispinosa 37,334 A2, 2 = A. brevispinosa 37,334 A1, 3 = A. arbustorum 86,813 A2, 4 = A. arbustorum 100,810 A1, 5 = D. polymorpha 101,850, 6 = D. polymorpha 83,846 B1, 7 = H. pomatia 74,402 B2, 8 = P. planorbis 84,060 A2, C = Negative control)
Amplicons, supplied by LGC Genomics GmbH, used in various techniques. Bioz Stars score: 92/100, based on 32 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/amplicons/product/LGC Genomics GmbH
Average 92 stars, based on 32 article reviews
Price from $9.99 to $1999.99
amplicons - by Bioz Stars, 2020-09
92/100 stars

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1) Product Images from "DNA analysis of molluscs from a museum wet collection: a comparison of different extraction methods"

Article Title: DNA analysis of molluscs from a museum wet collection: a comparison of different extraction methods

Journal: BMC Research Notes

doi: 10.1186/s13104-016-2147-7

Representative image of an agarose gel electrophoresis for the amplicons COI-lf and 16S-sf ( 1 = A. brevispinosa 37,334 A2, 2 = A. brevispinosa 37,334 A1, 3 = A. arbustorum 86,813 A2, 4 = A. arbustorum 100,810 A1, 5 = D. polymorpha 101,850, 6 = D. polymorpha 83,846 B1, 7 = H. pomatia 74,402 B2, 8 = P. planorbis 84,060 A2, C = Negative control)
Figure Legend Snippet: Representative image of an agarose gel electrophoresis for the amplicons COI-lf and 16S-sf ( 1 = A. brevispinosa 37,334 A2, 2 = A. brevispinosa 37,334 A1, 3 = A. arbustorum 86,813 A2, 4 = A. arbustorum 100,810 A1, 5 = D. polymorpha 101,850, 6 = D. polymorpha 83,846 B1, 7 = H. pomatia 74,402 B2, 8 = P. planorbis 84,060 A2, C = Negative control)

Techniques Used: Agarose Gel Electrophoresis, Negative Control

2) Product Images from "DNA analysis of molluscs from a museum wet collection: a comparison of different extraction methods"

Article Title: DNA analysis of molluscs from a museum wet collection: a comparison of different extraction methods

Journal: BMC Research Notes

doi: 10.1186/s13104-016-2147-7

Representative image of an agarose gel electrophoresis for the amplicons COI-lf and 16S-sf ( 1 = A. brevispinosa 37,334 A2, 2 = A. brevispinosa 37,334 A1, 3 = A. arbustorum 86,813 A2, 4 = A. arbustorum 100,810 A1, 5 = D. polymorpha 101,850, 6 = D. polymorpha 83,846 B1, 7 = H. pomatia 74,402 B2, 8 = P. planorbis 84,060 A2, C = Negative control)
Figure Legend Snippet: Representative image of an agarose gel electrophoresis for the amplicons COI-lf and 16S-sf ( 1 = A. brevispinosa 37,334 A2, 2 = A. brevispinosa 37,334 A1, 3 = A. arbustorum 86,813 A2, 4 = A. arbustorum 100,810 A1, 5 = D. polymorpha 101,850, 6 = D. polymorpha 83,846 B1, 7 = H. pomatia 74,402 B2, 8 = P. planorbis 84,060 A2, C = Negative control)

Techniques Used: Agarose Gel Electrophoresis, Negative Control

Related Articles

Clone Assay:

Article Title: Parasite fauna of wild Antillean manatees (Trichechus manatus manatus) of the Andean Region, Colombia
Article Snippet: .. Afterwards, the amplicons were cloned into pDrive vector (Qiagen) and the isolated recombinant plasmid DNA with amplicons was sequenced in both directions by LGC Genomics (Berlin, Germany). ..

Article Title: Nictaba Homologs from Arabidopsis thaliana Are Involved in Plant Stress Responses
Article Snippet: .. Specificity of the primers was tested in silico by BLAST search and amplicons were cloned and verified using agarose gel electrophoresis and sequencing (LGC Genomics, Berlin). .. Amplification efficiency of all primer pairs was determined in the CFX Manager 3.1 (Bio-Rad) and qBASEPLUS software ( ).

Amplification:

Article Title: Detection of equid herpesviruses among different Arabian horse populations in Egypt
Article Snippet: .. Fourteen randomly selected amplicons were purified and directly sequenced by Sanger sequencing (LGC Genomics) to confirm that the amplified fragments contain herpesvirus DNA. .. All DNA samples were re‐analysed by qPCR with the Applied Biosystems 7500 FAST (ABI, Foster City, CA) using specific primers and probes targeting the highly conserved gB gene (Table ) (Dynon et al .

Article Title: DNA analysis of molluscs from a museum wet collection: a comparison of different extraction methods
Article Snippet: .. To test whether the amplified amplicons are the expected gene sequences, a subset of samples was sequenced (LGC Genomics, Berlin) using the same primers used for producing the amplicons. ..

Agarose Gel Electrophoresis:

Article Title: Nictaba Homologs from Arabidopsis thaliana Are Involved in Plant Stress Responses
Article Snippet: .. Specificity of the primers was tested in silico by BLAST search and amplicons were cloned and verified using agarose gel electrophoresis and sequencing (LGC Genomics, Berlin). .. Amplification efficiency of all primer pairs was determined in the CFX Manager 3.1 (Bio-Rad) and qBASEPLUS software ( ).

Isolation:

Article Title: Parasite fauna of wild Antillean manatees (Trichechus manatus manatus) of the Andean Region, Colombia
Article Snippet: .. Afterwards, the amplicons were cloned into pDrive vector (Qiagen) and the isolated recombinant plasmid DNA with amplicons was sequenced in both directions by LGC Genomics (Berlin, Germany). ..

TA Cloning:

Article Title: Methanol oxidation by temperate soils and environmental determinants of associated methylotrophs
Article Snippet: .. These amplicons were pooled, and separated by TA cloning into competent Escherichia coli cells (LGC Genomics GmbH, Berlin, Germany). ..

Purification:

Article Title: Detection of equid herpesviruses among different Arabian horse populations in Egypt
Article Snippet: .. Fourteen randomly selected amplicons were purified and directly sequenced by Sanger sequencing (LGC Genomics) to confirm that the amplified fragments contain herpesvirus DNA. .. All DNA samples were re‐analysed by qPCR with the Applied Biosystems 7500 FAST (ABI, Foster City, CA) using specific primers and probes targeting the highly conserved gB gene (Table ) (Dynon et al .

In Silico:

Article Title: Nictaba Homologs from Arabidopsis thaliana Are Involved in Plant Stress Responses
Article Snippet: .. Specificity of the primers was tested in silico by BLAST search and amplicons were cloned and verified using agarose gel electrophoresis and sequencing (LGC Genomics, Berlin). .. Amplification efficiency of all primer pairs was determined in the CFX Manager 3.1 (Bio-Rad) and qBASEPLUS software ( ).

Sequencing:

Article Title: Detection of equid herpesviruses among different Arabian horse populations in Egypt
Article Snippet: .. Fourteen randomly selected amplicons were purified and directly sequenced by Sanger sequencing (LGC Genomics) to confirm that the amplified fragments contain herpesvirus DNA. .. All DNA samples were re‐analysed by qPCR with the Applied Biosystems 7500 FAST (ABI, Foster City, CA) using specific primers and probes targeting the highly conserved gB gene (Table ) (Dynon et al .

Article Title: Host-feeding patterns of mosquito species in Germany
Article Snippet: .. Sanger sequencing was applied for all positive amplicons (LGC Genomics, Berlin, Germany), sequences pre-processed with Geneious® 7.1.9 and compared to sequences from the GenBank database (National Center for Biotechnology Information, http://blast.ncbi.nlm.nih.gov/Blast.cgi ). ..

Article Title: The ORF012 Gene of Marek's Disease Virus Type 1 Produces a Spliced Transcript and Encodes a Novel Nuclear Phosphoprotein Essential for Virus Growth
Article Snippet: .. In addition, the amplicons were subjected to Sanger sequencing (LGC Genomics). .. Reaction mixtures to which no RT was added served as a control for genomic-DNA contamination, and amplification of chicken glyceraldehyde-3-phospate dehydrogenase (GAPDH) served as an internal control.

Article Title: Nictaba Homologs from Arabidopsis thaliana Are Involved in Plant Stress Responses
Article Snippet: .. Specificity of the primers was tested in silico by BLAST search and amplicons were cloned and verified using agarose gel electrophoresis and sequencing (LGC Genomics, Berlin). .. Amplification efficiency of all primer pairs was determined in the CFX Manager 3.1 (Bio-Rad) and qBASEPLUS software ( ).

Recombinant:

Article Title: Parasite fauna of wild Antillean manatees (Trichechus manatus manatus) of the Andean Region, Colombia
Article Snippet: .. Afterwards, the amplicons were cloned into pDrive vector (Qiagen) and the isolated recombinant plasmid DNA with amplicons was sequenced in both directions by LGC Genomics (Berlin, Germany). ..

Plasmid Preparation:

Article Title: Parasite fauna of wild Antillean manatees (Trichechus manatus manatus) of the Andean Region, Colombia
Article Snippet: .. Afterwards, the amplicons were cloned into pDrive vector (Qiagen) and the isolated recombinant plasmid DNA with amplicons was sequenced in both directions by LGC Genomics (Berlin, Germany). ..

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    LGC Genomics GmbH amplicons
    Representative image of an agarose gel electrophoresis for the <t>amplicons</t> COI-lf and 16S-sf ( 1 = A. brevispinosa 37,334 A2, 2 = A. brevispinosa 37,334 A1, 3 = A. arbustorum 86,813 A2, 4 = A. arbustorum 100,810 A1, 5 = D. polymorpha 101,850, 6 = D. polymorpha 83,846 B1, 7 = H. pomatia 74,402 B2, 8 = P. planorbis 84,060 A2, C = Negative control)
    Amplicons, supplied by LGC Genomics GmbH, used in various techniques. Bioz Stars score: 92/100, based on 32 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/amplicons/product/LGC Genomics GmbH
    Average 92 stars, based on 32 article reviews
    Price from $9.99 to $1999.99
    amplicons - by Bioz Stars, 2020-09
    92/100 stars
      Buy from Supplier

    90
    LGC Genomics GmbH 16s rrna gene amplicons
    Neighbour-joining tree based on almost complete <t>16S</t> <t>rRNA</t> gene sequences, showing the phylogenetic relationships between 81 Scottish and Antarctic bacterial isolates (KP011, KP122, KP130 and KP133 were excluded from the tree) with NCBI accession numbers in brackets. Bootstrap support > 50 % based on a neighbour-joining analysis of 1000 resampled datasets is shown. Scale bar, 0.1 substitutions per nucleotide position. * strains subjected to metabolite comparison through molecular networking. Branches are colour-coded according to their isolation location (Scotland, Green; Antarctica, Blue) for each bacterial isolate.
    16s Rrna Gene Amplicons, supplied by LGC Genomics GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/16s rrna gene amplicons/product/LGC Genomics GmbH
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    16s rrna gene amplicons - by Bioz Stars, 2020-09
    90/100 stars
      Buy from Supplier

    90
    LGC Genomics GmbH 16s rrna gene amplicon sequencing
    Principal coordinate analysis (PCoA) of unweighted UniFrac distances after transforming the bacterial <t>16S</t> <t>rRNA</t> gene OTU table to even sampling depth. Circle and triangle shapes represent spring and winter seasons, respectively. The different colors highlight the treatments along each season: plates without anti-corrosion paint in spring and winter, plates with anti-corrosion paint in spring and winter, and planktonic communities in spring and winter.
    16s Rrna Gene Amplicon Sequencing, supplied by LGC Genomics GmbH, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/16s rrna gene amplicon sequencing/product/LGC Genomics GmbH
    Average 90 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    16s rrna gene amplicon sequencing - by Bioz Stars, 2020-09
    90/100 stars
      Buy from Supplier

    Image Search Results


    Representative image of an agarose gel electrophoresis for the amplicons COI-lf and 16S-sf ( 1 = A. brevispinosa 37,334 A2, 2 = A. brevispinosa 37,334 A1, 3 = A. arbustorum 86,813 A2, 4 = A. arbustorum 100,810 A1, 5 = D. polymorpha 101,850, 6 = D. polymorpha 83,846 B1, 7 = H. pomatia 74,402 B2, 8 = P. planorbis 84,060 A2, C = Negative control)

    Journal: BMC Research Notes

    Article Title: DNA analysis of molluscs from a museum wet collection: a comparison of different extraction methods

    doi: 10.1186/s13104-016-2147-7

    Figure Lengend Snippet: Representative image of an agarose gel electrophoresis for the amplicons COI-lf and 16S-sf ( 1 = A. brevispinosa 37,334 A2, 2 = A. brevispinosa 37,334 A1, 3 = A. arbustorum 86,813 A2, 4 = A. arbustorum 100,810 A1, 5 = D. polymorpha 101,850, 6 = D. polymorpha 83,846 B1, 7 = H. pomatia 74,402 B2, 8 = P. planorbis 84,060 A2, C = Negative control)

    Article Snippet: To test whether the amplified amplicons are the expected gene sequences, a subset of samples was sequenced (LGC Genomics, Berlin) using the same primers used for producing the amplicons.

    Techniques: Agarose Gel Electrophoresis, Negative Control

    Neighbour-joining tree based on almost complete 16S rRNA gene sequences, showing the phylogenetic relationships between 81 Scottish and Antarctic bacterial isolates (KP011, KP122, KP130 and KP133 were excluded from the tree) with NCBI accession numbers in brackets. Bootstrap support > 50 % based on a neighbour-joining analysis of 1000 resampled datasets is shown. Scale bar, 0.1 substitutions per nucleotide position. * strains subjected to metabolite comparison through molecular networking. Branches are colour-coded according to their isolation location (Scotland, Green; Antarctica, Blue) for each bacterial isolate.

    Journal: Metabolites

    Article Title: Using Molecular Networking for Microbial Secondary Metabolite Bioprospecting

    doi: 10.3390/metabo6010002

    Figure Lengend Snippet: Neighbour-joining tree based on almost complete 16S rRNA gene sequences, showing the phylogenetic relationships between 81 Scottish and Antarctic bacterial isolates (KP011, KP122, KP130 and KP133 were excluded from the tree) with NCBI accession numbers in brackets. Bootstrap support > 50 % based on a neighbour-joining analysis of 1000 resampled datasets is shown. Scale bar, 0.1 substitutions per nucleotide position. * strains subjected to metabolite comparison through molecular networking. Branches are colour-coded according to their isolation location (Scotland, Green; Antarctica, Blue) for each bacterial isolate.

    Article Snippet: 16S rRNA gene amplicons were sequenced by LGC Genomics (Germany) using the primers 27f and 1492r.

    Techniques: Isolation

    Principal coordinate analysis (PCoA) of unweighted UniFrac distances after transforming the bacterial 16S rRNA gene OTU table to even sampling depth. Circle and triangle shapes represent spring and winter seasons, respectively. The different colors highlight the treatments along each season: plates without anti-corrosion paint in spring and winter, plates with anti-corrosion paint in spring and winter, and planktonic communities in spring and winter.

    Journal: Frontiers in Microbiology

    Article Title: Distinct Temporal Succession of Bacterial Communities in Early Marine Biofilms in a Portuguese Atlantic Port

    doi: 10.3389/fmicb.2020.01938

    Figure Lengend Snippet: Principal coordinate analysis (PCoA) of unweighted UniFrac distances after transforming the bacterial 16S rRNA gene OTU table to even sampling depth. Circle and triangle shapes represent spring and winter seasons, respectively. The different colors highlight the treatments along each season: plates without anti-corrosion paint in spring and winter, plates with anti-corrosion paint in spring and winter, and planktonic communities in spring and winter.

    Article Snippet: DNA Sequencing and Sequence Analysis The 16S rRNA gene amplicon sequencing was performed by LGC Genomics (LGC Genomics GmbH, Berlin, Germany) using 300 bp paired-end reads, and sequencing the V3 region on an Illumina MiSeq sequencer (Illumina, Inc., San Diego, CA, United States) using the universal 16SrRNA bacterial primers 341F and 785R ( ).

    Techniques: Sampling