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Advanced Analytical Inc amplicons
Amplicons, supplied by Advanced Analytical Inc, used in various techniques. Bioz Stars score: 92/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/amplicons/product/Advanced Analytical Inc
Average 92 stars, based on 4 article reviews
Price from $9.99 to $1999.99
amplicons - by Bioz Stars, 2020-09
92/100 stars

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Polymerase Chain Reaction:

Article Title: Sequencing Framework for the Sensitive Detection and Precise Mapping of Defective Interfering Particle-Associated Deletions across Influenza A and B Viruses
Article Snippet: .. To confirm the PCR products, we visualized the amplicons on a Fragment Analyzer (AATI) with the DNF-486 high-sensitivity NGS kit before and after fragmentation. .. Next, we used a Kapa Hyper prep kit (Roche) according to the manual to construct the libraries.

Article Title: Host genetics and the rumen microbiome jointly associate with methane emissions in dairy cows
Article Snippet: .. Multiplex indices and Illumina overhang adapters were added to both amplicon libraries in a second PCR amplification round (six cycles), followed by Fragment Analyzer analysis to confirm the correct size of the amplicons (Advanced Analytical Technologies, Ankeny, USA). .. Ninety-six libraries were pooled in equimolar concentrations and sequenced with an Illumina sequencing instrument using the 300 bp paired-end read mode, according to the manufacturer’s specifications.

Article Title: Illumina-based sequencing framework for accurate detection and mapping of influenza virus defective interfering particle-associated RNAs
Article Snippet: .. To confirm the PCR products, we visualized the amplicons on a Fragment Analyzer (AATI) with the DNF-486 high sensitivity NGS kit before and after fragmentation. .. Next, we used KAPA Hyper Prep kit (Roche) to construct the libraries according to the manual.

IA:

Article Title: Microbiota stability in healthy individuals after single-dose lactulose challenge—A randomized controlled study
Article Snippet: .. The quality of the amplicons was evaluated using a Fragment Analyzer (Advanced Analytical, Ankeny, IA, USA). ..

Next-Generation Sequencing:

Article Title: Responses of the Differentiated Intestinal Epithelial Cell Line Caco-2 to Infection With the Giardia intestinalis GS Isolate
Article Snippet: .. Amplicons were quantified using the Fragment AnalyzerTM instrument (Advanced Analytical Technologies, INC.) with the DNF-474 High Sensitivity NGS Fragment Analysis Kit (Advanced Analytical Technologies, INC.). .. Samples were then pooled (8 samples per pool) followed by emulsion PCR on the Ion OneTouch™ 2 system with the Ion PI™ Template OT2 200 Kit v3 (Thermo Fisher Scientific) and enrichment using the Ion OneTouch™ ES (Thermo Fisher Scientific).

Article Title: Sequencing Framework for the Sensitive Detection and Precise Mapping of Defective Interfering Particle-Associated Deletions across Influenza A and B Viruses
Article Snippet: .. To confirm the PCR products, we visualized the amplicons on a Fragment Analyzer (AATI) with the DNF-486 high-sensitivity NGS kit before and after fragmentation. .. Next, we used a Kapa Hyper prep kit (Roche) according to the manual to construct the libraries.

Article Title: Illumina-based sequencing framework for accurate detection and mapping of influenza virus defective interfering particle-associated RNAs
Article Snippet: .. To confirm the PCR products, we visualized the amplicons on a Fragment Analyzer (AATI) with the DNF-486 high sensitivity NGS kit before and after fragmentation. .. Next, we used KAPA Hyper Prep kit (Roche) to construct the libraries according to the manual.

Amplification:

Article Title: Host genetics and the rumen microbiome jointly associate with methane emissions in dairy cows
Article Snippet: .. Multiplex indices and Illumina overhang adapters were added to both amplicon libraries in a second PCR amplification round (six cycles), followed by Fragment Analyzer analysis to confirm the correct size of the amplicons (Advanced Analytical Technologies, Ankeny, USA). .. Ninety-six libraries were pooled in equimolar concentrations and sequenced with an Illumina sequencing instrument using the 300 bp paired-end read mode, according to the manufacturer’s specifications.

Multiplex Assay:

Article Title: Host genetics and the rumen microbiome jointly associate with methane emissions in dairy cows
Article Snippet: .. Multiplex indices and Illumina overhang adapters were added to both amplicon libraries in a second PCR amplification round (six cycles), followed by Fragment Analyzer analysis to confirm the correct size of the amplicons (Advanced Analytical Technologies, Ankeny, USA). .. Ninety-six libraries were pooled in equimolar concentrations and sequenced with an Illumina sequencing instrument using the 300 bp paired-end read mode, according to the manufacturer’s specifications.

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  • 91
    Advanced Analytical Inc pcr amplicons
    Examples of COS markers resulting in polymorphic <t>PCR</t> products between hexaploid wheat and perennial grasses. (A) <t>Amplicons</t> produced by the markers c746642 and (B) c756279 from genomic DNA of wheat genotype GK Öthalom ( T . aestivum ), T . elongatum MvGB1963, T . bessarabicum MvGB1706, Ps . spicata MvGB1607 and MvGB1615, and A . cristatum MvGB1521 and MvGB1509.
    Pcr Amplicons, supplied by Advanced Analytical Inc, used in various techniques. Bioz Stars score: 91/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcr amplicons/product/Advanced Analytical Inc
    Average 91 stars, based on 11 article reviews
    Price from $9.99 to $1999.99
    pcr amplicons - by Bioz Stars, 2020-09
    91/100 stars
      Buy from Supplier

    91
    Advanced Analytical Inc amplicon size
    Microbiota affects development and survival differently in Selected and Control populations on poor diet. (A) Identities and relative abundances of the 5 most abundant taxa in Selected and Control larvae reared in their conventional media, assigned by 16S rRNA gene <t>amplicon</t> sequencing. (B) Mean egg-to-pupa development time in Selected and Control populations, with or without microbiota. (C) Mean egg-to-pupa survival rate under the same conditions. Symbols and error bars represent means ± standard errors of the means for each population (where error bars are not visible, they are smaller than the symbols). Black horizontal bars represent the means for the six replicate populations. Main effect differences analyzed by GMM are represented in the panels. Interaction = colonization × regime. Detailed statistics are presented in Table S1 in the supplemental material.
    Amplicon Size, supplied by Advanced Analytical Inc, used in various techniques. Bioz Stars score: 91/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/amplicon size/product/Advanced Analytical Inc
    Average 91 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    amplicon size - by Bioz Stars, 2020-09
    91/100 stars
      Buy from Supplier

    Image Search Results


    Examples of COS markers resulting in polymorphic PCR products between hexaploid wheat and perennial grasses. (A) Amplicons produced by the markers c746642 and (B) c756279 from genomic DNA of wheat genotype GK Öthalom ( T . aestivum ), T . elongatum MvGB1963, T . bessarabicum MvGB1706, Ps . spicata MvGB1607 and MvGB1615, and A . cristatum MvGB1521 and MvGB1509.

    Journal: PLoS ONE

    Article Title: Molecular cytogenetic (FISH) and genome analysis of diploid wheatgrasses and their phylogenetic relationship

    doi: 10.1371/journal.pone.0173623

    Figure Lengend Snippet: Examples of COS markers resulting in polymorphic PCR products between hexaploid wheat and perennial grasses. (A) Amplicons produced by the markers c746642 and (B) c756279 from genomic DNA of wheat genotype GK Öthalom ( T . aestivum ), T . elongatum MvGB1963, T . bessarabicum MvGB1706, Ps . spicata MvGB1607 and MvGB1615, and A . cristatum MvGB1521 and MvGB1509.

    Article Snippet: PCR amplicons have been separated by a Fragment Analyzer Automated CE System equipped with a 96-Capillary Array Cartridge (effective length 33 cm) (Advanced Analytical Technologies, Ames, USA) and analyzed using PROsize v2.0 software.

    Techniques: Polymerase Chain Reaction, Produced

    Microbiota affects development and survival differently in Selected and Control populations on poor diet. (A) Identities and relative abundances of the 5 most abundant taxa in Selected and Control larvae reared in their conventional media, assigned by 16S rRNA gene amplicon sequencing. (B) Mean egg-to-pupa development time in Selected and Control populations, with or without microbiota. (C) Mean egg-to-pupa survival rate under the same conditions. Symbols and error bars represent means ± standard errors of the means for each population (where error bars are not visible, they are smaller than the symbols). Black horizontal bars represent the means for the six replicate populations. Main effect differences analyzed by GMM are represented in the panels. Interaction = colonization × regime. Detailed statistics are presented in Table S1 in the supplemental material.

    Journal: mBio

    Article Title: Adaptation to Chronic Nutritional Stress Leads to Reduced Dependence on Microbiota in Drosophila melanogaster

    doi: 10.1128/mBio.01496-17

    Figure Lengend Snippet: Microbiota affects development and survival differently in Selected and Control populations on poor diet. (A) Identities and relative abundances of the 5 most abundant taxa in Selected and Control larvae reared in their conventional media, assigned by 16S rRNA gene amplicon sequencing. (B) Mean egg-to-pupa development time in Selected and Control populations, with or without microbiota. (C) Mean egg-to-pupa survival rate under the same conditions. Symbols and error bars represent means ± standard errors of the means for each population (where error bars are not visible, they are smaller than the symbols). Black horizontal bars represent the means for the six replicate populations. Main effect differences analyzed by GMM are represented in the panels. Interaction = colonization × regime. Detailed statistics are presented in Table S1 in the supplemental material.

    Article Snippet: Three replicate 25-µl PCR mixtures containing 10 ng µl−1 DNA and 1 µM (each) primer were carried out under the following conditions: 95°C for 3 min and 25 cycles of 95°C for 30 s, 56°C for 15 s, and 72°C for 30 s, followed by a final incubation at 72°C for 5 min. Products were pooled from triplicate reaction mixtures and verified for amplicon size on a Fragment Analyzer (Advanced Analytical Technologies, Inc.).

    Techniques: Amplification, Sequencing

    Microbiota of Selected and Control populations. (A) Identities and relative abundances of the 5 most abundant taxa in the mixed adult feces (used as the source of inoculum) and in the larval poor medium of Selected (S) and Control (C) populations previously colonized with that inoculum, assigned by 16S rRNA gene amplicon sequencing. (B) Abundance of Acetobacteraceae relative to the host DNA in GF, Acetobacter -monoassociated, and conventionally reared (as in the experimental evolution) Selected and Control populations measured by qPCR. Symbols represent means ± standard errors of the means for each population. Black bars represent the mean for the six populations within each regime. Main effect differences analyzed by GMM are represented in the panel. Interaction refers to colonization × evolutionary regime. Details are presented in Table S4 .

    Journal: mBio

    Article Title: Adaptation to Chronic Nutritional Stress Leads to Reduced Dependence on Microbiota in Drosophila melanogaster

    doi: 10.1128/mBio.01496-17

    Figure Lengend Snippet: Microbiota of Selected and Control populations. (A) Identities and relative abundances of the 5 most abundant taxa in the mixed adult feces (used as the source of inoculum) and in the larval poor medium of Selected (S) and Control (C) populations previously colonized with that inoculum, assigned by 16S rRNA gene amplicon sequencing. (B) Abundance of Acetobacteraceae relative to the host DNA in GF, Acetobacter -monoassociated, and conventionally reared (as in the experimental evolution) Selected and Control populations measured by qPCR. Symbols represent means ± standard errors of the means for each population. Black bars represent the mean for the six populations within each regime. Main effect differences analyzed by GMM are represented in the panel. Interaction refers to colonization × evolutionary regime. Details are presented in Table S4 .

    Article Snippet: Three replicate 25-µl PCR mixtures containing 10 ng µl−1 DNA and 1 µM (each) primer were carried out under the following conditions: 95°C for 3 min and 25 cycles of 95°C for 30 s, 56°C for 15 s, and 72°C for 30 s, followed by a final incubation at 72°C for 5 min. Products were pooled from triplicate reaction mixtures and verified for amplicon size on a Fragment Analyzer (Advanced Analytical Technologies, Inc.).

    Techniques: Amplification, Sequencing, Real-time Polymerase Chain Reaction

    Principal coordinate analysis (PCoA) of rumen bacterial community diversity (a) and archaeal community diversity (b) based on 16S rRNA amplicon sequencing contrasting 10% highest methane emitters (orange), 10% lowest methane emitters (blue), and 80% intermediate emitters (grey). Distribution of high and low emitters along PCo1 showed significant differences ( P

    Journal: PLoS Genetics

    Article Title: Host genetics and the rumen microbiome jointly associate with methane emissions in dairy cows

    doi: 10.1371/journal.pgen.1007580

    Figure Lengend Snippet: Principal coordinate analysis (PCoA) of rumen bacterial community diversity (a) and archaeal community diversity (b) based on 16S rRNA amplicon sequencing contrasting 10% highest methane emitters (orange), 10% lowest methane emitters (blue), and 80% intermediate emitters (grey). Distribution of high and low emitters along PCo1 showed significant differences ( P

    Article Snippet: Multiplex indices and Illumina overhang adapters were added to both amplicon libraries in a second PCR amplification round (six cycles), followed by Fragment Analyzer analysis to confirm the correct size of the amplicons (Advanced Analytical Technologies, Ankeny, USA).

    Techniques: Amplification, Sequencing

    Species identified within the products using amplicon metabarcoding (AMB). Only MOTUs with > 1% normalized read numbers per sample are shown. Species are colored according to relative abundance of normalized read numbers. Products are grouped by product form: herbal teas, capsules, tablets/pills/pastilles and extracts/tinctures/oils.

    Journal: Scientific Reports

    Article Title: Comparative authentication of Hypericum perforatum herbal products using DNA metabarcoding, TLC and HPLC-MS

    doi: 10.1038/s41598-017-01389-w

    Figure Lengend Snippet: Species identified within the products using amplicon metabarcoding (AMB). Only MOTUs with > 1% normalized read numbers per sample are shown. Species are colored according to relative abundance of normalized read numbers. Products are grouped by product form: herbal teas, capsules, tablets/pills/pastilles and extracts/tinctures/oils.

    Article Snippet: The total concentration of the purified pooled amplicon library stock and three serial dilutions (undiluted, 1/5, 1/10) were analyzed using the Fragment Analyzer™ (Advanced Analytical Technologies, Inc., USA) and DNF-488 High Sensitivity Genomic DNA Analysis Kit in order to identify the optimum concentration range for the template preparation.

    Techniques: Amplification

    Presence of Hypericum perforatum within the products. ( A ) Detection using thin layer chromatogram (TLC). ( B ) Detection using high-performance liquid chromatography-mass spectrometry (HPLC-MS). ( C ) Detection using amplicon metabarcoding (AMB). Detection between methods is not fully comparable as the resolution of the approaches differs.

    Journal: Scientific Reports

    Article Title: Comparative authentication of Hypericum perforatum herbal products using DNA metabarcoding, TLC and HPLC-MS

    doi: 10.1038/s41598-017-01389-w

    Figure Lengend Snippet: Presence of Hypericum perforatum within the products. ( A ) Detection using thin layer chromatogram (TLC). ( B ) Detection using high-performance liquid chromatography-mass spectrometry (HPLC-MS). ( C ) Detection using amplicon metabarcoding (AMB). Detection between methods is not fully comparable as the resolution of the approaches differs.

    Article Snippet: The total concentration of the purified pooled amplicon library stock and three serial dilutions (undiluted, 1/5, 1/10) were analyzed using the Fragment Analyzer™ (Advanced Analytical Technologies, Inc., USA) and DNF-488 High Sensitivity Genomic DNA Analysis Kit in order to identify the optimum concentration range for the template preparation.

    Techniques: Thin Layer Chromatography, High Performance Liquid Chromatography, Mass Spectrometry, Amplification