Structured Review

Bio-Rad amplicon
Amplicon, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 83 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/amplicon/product/Bio-Rad
Average 93 stars, based on 83 article reviews
Price from $9.99 to $1999.99
amplicon - by Bioz Stars, 2020-07
93/100 stars

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Related Articles

Amplification:

Article Title: Upregulated effects of miR-7 in methicillin-resistant Staphylococcus aureus
Article Snippet: .. The reaction conditions were: Pre-denaturation at 94°C for 4 min, denaturation at 94°C for 30 sec, annealing at 56°C for 30 sec, and extension at 72°C for 1 min. After 35 cycles, extended once more at 72°C for 10 min. For sequence analysis, amplicon was extracted for agarose gel electrophoresis, 5 µl PCR amplicon for each well, with a voltage 110 V for 40 min. After electrophoresis, agarose gel was observed in ultraviolet spectrophotometer (Bio-Rad, Hercules, CA, USA). ..

Agarose Gel Electrophoresis:

Article Title: Upregulated effects of miR-7 in methicillin-resistant Staphylococcus aureus
Article Snippet: .. The reaction conditions were: Pre-denaturation at 94°C for 4 min, denaturation at 94°C for 30 sec, annealing at 56°C for 30 sec, and extension at 72°C for 1 min. After 35 cycles, extended once more at 72°C for 10 min. For sequence analysis, amplicon was extracted for agarose gel electrophoresis, 5 µl PCR amplicon for each well, with a voltage 110 V for 40 min. After electrophoresis, agarose gel was observed in ultraviolet spectrophotometer (Bio-Rad, Hercules, CA, USA). ..

Size-exclusion Chromatography:

Article Title: Upregulated effects of miR-7 in methicillin-resistant Staphylococcus aureus
Article Snippet: .. The reaction conditions were: Pre-denaturation at 94°C for 4 min, denaturation at 94°C for 30 sec, annealing at 56°C for 30 sec, and extension at 72°C for 1 min. After 35 cycles, extended once more at 72°C for 10 min. For sequence analysis, amplicon was extracted for agarose gel electrophoresis, 5 µl PCR amplicon for each well, with a voltage 110 V for 40 min. After electrophoresis, agarose gel was observed in ultraviolet spectrophotometer (Bio-Rad, Hercules, CA, USA). ..

Spectrophotometry:

Article Title: Upregulated effects of miR-7 in methicillin-resistant Staphylococcus aureus
Article Snippet: .. The reaction conditions were: Pre-denaturation at 94°C for 4 min, denaturation at 94°C for 30 sec, annealing at 56°C for 30 sec, and extension at 72°C for 1 min. After 35 cycles, extended once more at 72°C for 10 min. For sequence analysis, amplicon was extracted for agarose gel electrophoresis, 5 µl PCR amplicon for each well, with a voltage 110 V for 40 min. After electrophoresis, agarose gel was observed in ultraviolet spectrophotometer (Bio-Rad, Hercules, CA, USA). ..

Electrophoresis:

Article Title: Upregulated effects of miR-7 in methicillin-resistant Staphylococcus aureus
Article Snippet: .. The reaction conditions were: Pre-denaturation at 94°C for 4 min, denaturation at 94°C for 30 sec, annealing at 56°C for 30 sec, and extension at 72°C for 1 min. After 35 cycles, extended once more at 72°C for 10 min. For sequence analysis, amplicon was extracted for agarose gel electrophoresis, 5 µl PCR amplicon for each well, with a voltage 110 V for 40 min. After electrophoresis, agarose gel was observed in ultraviolet spectrophotometer (Bio-Rad, Hercules, CA, USA). ..

Sequencing:

Article Title: Upregulated effects of miR-7 in methicillin-resistant Staphylococcus aureus
Article Snippet: .. The reaction conditions were: Pre-denaturation at 94°C for 4 min, denaturation at 94°C for 30 sec, annealing at 56°C for 30 sec, and extension at 72°C for 1 min. After 35 cycles, extended once more at 72°C for 10 min. For sequence analysis, amplicon was extracted for agarose gel electrophoresis, 5 µl PCR amplicon for each well, with a voltage 110 V for 40 min. After electrophoresis, agarose gel was observed in ultraviolet spectrophotometer (Bio-Rad, Hercules, CA, USA). ..

other:

Article Title: A Simple and Efficient Approach to Elucidate Genomic Contribution of Transcripts to a Target Gene in Polyploids: The Case of Hexaploid Wheat (Triticum aestivum L.)
Article Snippet: Quantitation of band intensity The area, volume, and intensity of each band corresponding to expected size of a given amplicon were analyzed using Quantity One Software (Bio-Rad) and the global background subtraction method.

Article Title: Cholera returns to southern Vietnam in an outbreak associated with consuming unsafe water through iced tea: A matched case-control study
Article Snippet: A 9 μl of amplicon was separated by electrophoresis on a 1.5% agarose gel and visualized with an ultraviolet light on the Gel Doc System (Bio-Rad Laboratories).

Article Title: Ecdysone-Related Biomarkers of Toxicity in the Model Organism Chironomus riparius: Stage and Sex-Dependent Variations in Gene Expression Profiles
Article Snippet: Real-Time PCR was run in the following cycling conditions: initial denaturation at 95°C for 3 min, 35 cycles of 95°C denaturation for 5 s, 58°C annealing for 15 s and 65°C elongation for 10 s. To verify the accuracy of each amplicon, a melting curve analysis was carried out after amplification BioRad CFX Manager 2.1 software was used to calculate the mRNA levels by the normalized gene expression (2-ΔΔCT ) against three endogenous reference genes (26S, actin and GAPDH ).

Article Title: Russian isolates enlarge the known geographic diversity of Francisella tularensis subsp. mediasiatica
Article Snippet: Also in these few cases we confirmed the size of amplicon using Experion™ Automated Electrophoresis System (BioRad, Hercules, USA) and by sequencing the fragment, followed by a direct count of the number of repeats.

Article Title: T cell receptor CDR2? and CDR3? loops collaborate functionally to shape the iNKT cell repertoire
Article Snippet: Reverse transcription was carried out by using the SuperScript III kit (Invitrogen) and the amount of amplicon generated was monitored using a DNA engine Opticon 2 apparatus (Bio-Rad) with gene specific primers and probes and the Platinum Quantitative PCR SuperMix UDG (Invitrogen).

Article Title: Re-annotation of the physical map of Glycine max for polyploid-like regions by BAC end sequence driven whole genome shotgun read assembly
Article Snippet: An initial 95°C denaturation for 5 min was followed by 30 cycles of 95° for 30 s, 55° for 30 s, and 72° for 30 s. After PCR was complete, gel electrophoresis was performed in a 2% (w/v) agarose gel or a 4% (w/v) PAGE stained with ethidium bromide and amplicon documented using a BioRad GelDoc (Hercules, CA) system.

Article Title: Virome analyses of Hevea brasiliensis using small RNA deep sequencing and PCR techniques reveal the presence of a potential new virus
Article Snippet: The amplicon quantity was evaluated using QuantaSoft version 1.7 (BIO-RAD, Hercules, CA, USA) by determining the threshold value and the number of positive copies of the target in a 20 μL reaction.

Polymerase Chain Reaction:

Article Title: Upregulated effects of miR-7 in methicillin-resistant Staphylococcus aureus
Article Snippet: .. The reaction conditions were: Pre-denaturation at 94°C for 4 min, denaturation at 94°C for 30 sec, annealing at 56°C for 30 sec, and extension at 72°C for 1 min. After 35 cycles, extended once more at 72°C for 10 min. For sequence analysis, amplicon was extracted for agarose gel electrophoresis, 5 µl PCR amplicon for each well, with a voltage 110 V for 40 min. After electrophoresis, agarose gel was observed in ultraviolet spectrophotometer (Bio-Rad, Hercules, CA, USA). ..

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  • 90
    Bio-Rad tirrar tcpc amplicons
    C-terminal Arg-Arg-Arg motif is critical for translocation across cell membrane. CD11c + dendritic cells or CD4 + CD62L + (naïve) T-cells isolated from spleen of C57BL6 / J mice (male, 4–6 weeks old) were treated with <t>FITC-TIR-TcpC</t> ( TIR ), <t>FITC-TIRRAR-TcpC</t>
    Tirrar Tcpc Amplicons, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tirrar tcpc amplicons/product/Bio-Rad
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
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    92
    Bio-Rad 16s rrna amplicons
    Distribution of bacterial phylotypes in the cecum content and fecal pellets of mice exposed for 8 weeks to Cd (20 or 100 ppm) or Pb (100 or 500 ppm) salts via their drinking water. <t>16S</t> <t>rRNA-base</t> analyses were derived from 454/Roche multitag pyrosequencing. Data are expressed as the mean percentage abundance of the total assignment (n = 5 animals per group). In line with the literature data, most of the bacteria in untreated (control) mice belonged to Firmicutes or the Bacteroidetes, whereas Actinobacteria were very rare.
    16s Rrna Amplicons, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 92/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/16s rrna amplicons/product/Bio-Rad
    Average 92 stars, based on 12 article reviews
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    91
    Bio-Rad template amplicon probe signals
    DSB-ddPCR is an accurate and precise way to quantify DSBs (a) A schematic illustrates the DSB-ddPCR assay. The assay relies on two locus-specific PCR amplicons, the first of which spans the cleavage junction (primers F1, R1) and the second of which is adjacent (primers F2, R2). Amplicons are detected by the activation of <t>amplicon-specific</t> fluorescent hydrolysis probes. Template that has been cleaved by Cas9 or another nuclease does not generate the first amplicon or activate the first probe. Uncleaved control DNA was digested with a restriction enzyme to create cleaved control DNA. Uncleaved and cleaved control DNA was mixed in specific proportions. (b) Representative droplet FAM and VIC probe intensities are shown for uncleaved control MYC locus template DNA (left panel), a 1:1 mixture of uncleaved:cleaved control DNA (middle panel), and cleaved control DNA (right panel). Colors indicate droplets with no template (gray), intact template (blue), and cleaved template (red). Droplet populations in the dot plots are: uncleaved control DNA (DSB: 120, no DSB: 1,724, no template: 12,360), 1:1 Mixture (DSB: 799, no DSB: 914, no template: 12,305), cleaved control DNA (DSB: 1,757, no DSB: 14, no template: 13,247).
    Template Amplicon Probe Signals, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 91/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/template amplicon probe signals/product/Bio-Rad
    Average 91 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    template amplicon probe signals - by Bioz Stars, 2020-07
    91/100 stars
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    94
    Bio-Rad pcr amplicons
    <t>PCR-single-strand</t> conformational polymorphism of the ovine IGF-1 gene. <t>Amplicons</t> were electrophoresed on a 12% non-denaturing acrylamide/bis-acrylamide gel; 200 V, 4 °C for 12 h. Three SSCP patterns, GG, AA and GA were detected.
    Pcr Amplicons, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 94/100, based on 174 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    C-terminal Arg-Arg-Arg motif is critical for translocation across cell membrane. CD11c + dendritic cells or CD4 + CD62L + (naïve) T-cells isolated from spleen of C57BL6 / J mice (male, 4–6 weeks old) were treated with FITC-TIR-TcpC ( TIR ), FITC-TIRRAR-TcpC

    Journal: The Journal of Biological Chemistry

    Article Title: Toll/Interleukin-1 Receptor Domain Derived from TcpC (TIR-TcpC) Ameliorates Experimental Autoimmune Arthritis by Down-modulating Th17 Cell Response *

    doi: 10.1074/jbc.M116.722801

    Figure Lengend Snippet: C-terminal Arg-Arg-Arg motif is critical for translocation across cell membrane. CD11c + dendritic cells or CD4 + CD62L + (naïve) T-cells isolated from spleen of C57BL6 / J mice (male, 4–6 weeks old) were treated with FITC-TIR-TcpC ( TIR ), FITC-TIRRAR-TcpC

    Article Snippet: The amplified products (TIR-TcpC and TIRRAR-TcpC amplicons) were cloned into pPAL7 expression vector (Bio-Rad) using a restriction endonuclease-free cloning strategy.

    Techniques: Translocation Assay, Isolation, Mouse Assay

    Distribution of bacterial phylotypes in the cecum content and fecal pellets of mice exposed for 8 weeks to Cd (20 or 100 ppm) or Pb (100 or 500 ppm) salts via their drinking water. 16S rRNA-base analyses were derived from 454/Roche multitag pyrosequencing. Data are expressed as the mean percentage abundance of the total assignment (n = 5 animals per group). In line with the literature data, most of the bacteria in untreated (control) mice belonged to Firmicutes or the Bacteroidetes, whereas Actinobacteria were very rare.

    Journal: BMC Pharmacology & Toxicology

    Article Title: Ecotoxicology inside the gut: impact of heavy metals on the mouse microbiome

    doi: 10.1186/2050-6511-14-62

    Figure Lengend Snippet: Distribution of bacterial phylotypes in the cecum content and fecal pellets of mice exposed for 8 weeks to Cd (20 or 100 ppm) or Pb (100 or 500 ppm) salts via their drinking water. 16S rRNA-base analyses were derived from 454/Roche multitag pyrosequencing. Data are expressed as the mean percentage abundance of the total assignment (n = 5 animals per group). In line with the literature data, most of the bacteria in untreated (control) mice belonged to Firmicutes or the Bacteroidetes, whereas Actinobacteria were very rare.

    Article Snippet: The resulting 16S rRNA amplicons were analyzed by DGGE fingerprinting analysis (the D-Code System from Bio-Rad, Nazareth, Belgium) using 35% to 70% denaturing gels, as previously described [ ].

    Techniques: Mouse Assay, Derivative Assay

    Distribution of bacterial subgroups in the cecum content and fecal pellets of mice exposed for 8 weeks to Cd (20 or 100 mg L -1 ) or Pb (100 or 500 mg L -1 ) salts via their drinking water. (A) Family-level and (B) genus-level. 16S rRNA-base analyses were derived from 454/Roche multitag pyrosequencing. Data are expressed as the mean percentage abundance (n = 5 animals per group). Only operational taxonomic units (OTU’s) present in dominant families ( > 0.1%) were considered. *: p

    Journal: BMC Pharmacology & Toxicology

    Article Title: Ecotoxicology inside the gut: impact of heavy metals on the mouse microbiome

    doi: 10.1186/2050-6511-14-62

    Figure Lengend Snippet: Distribution of bacterial subgroups in the cecum content and fecal pellets of mice exposed for 8 weeks to Cd (20 or 100 mg L -1 ) or Pb (100 or 500 mg L -1 ) salts via their drinking water. (A) Family-level and (B) genus-level. 16S rRNA-base analyses were derived from 454/Roche multitag pyrosequencing. Data are expressed as the mean percentage abundance (n = 5 animals per group). Only operational taxonomic units (OTU’s) present in dominant families ( > 0.1%) were considered. *: p

    Article Snippet: The resulting 16S rRNA amplicons were analyzed by DGGE fingerprinting analysis (the D-Code System from Bio-Rad, Nazareth, Belgium) using 35% to 70% denaturing gels, as previously described [ ].

    Techniques: Mouse Assay, Derivative Assay

    DSB-ddPCR is an accurate and precise way to quantify DSBs (a) A schematic illustrates the DSB-ddPCR assay. The assay relies on two locus-specific PCR amplicons, the first of which spans the cleavage junction (primers F1, R1) and the second of which is adjacent (primers F2, R2). Amplicons are detected by the activation of amplicon-specific fluorescent hydrolysis probes. Template that has been cleaved by Cas9 or another nuclease does not generate the first amplicon or activate the first probe. Uncleaved control DNA was digested with a restriction enzyme to create cleaved control DNA. Uncleaved and cleaved control DNA was mixed in specific proportions. (b) Representative droplet FAM and VIC probe intensities are shown for uncleaved control MYC locus template DNA (left panel), a 1:1 mixture of uncleaved:cleaved control DNA (middle panel), and cleaved control DNA (right panel). Colors indicate droplets with no template (gray), intact template (blue), and cleaved template (red). Droplet populations in the dot plots are: uncleaved control DNA (DSB: 120, no DSB: 1,724, no template: 12,360), 1:1 Mixture (DSB: 799, no DSB: 914, no template: 12,305), cleaved control DNA (DSB: 1,757, no DSB: 14, no template: 13,247).

    Journal: Nature methods

    Article Title: Rapidly inducible Cas9 and DSB-ddPCR to probe editing kinetics

    doi: 10.1038/nmeth.4368

    Figure Lengend Snippet: DSB-ddPCR is an accurate and precise way to quantify DSBs (a) A schematic illustrates the DSB-ddPCR assay. The assay relies on two locus-specific PCR amplicons, the first of which spans the cleavage junction (primers F1, R1) and the second of which is adjacent (primers F2, R2). Amplicons are detected by the activation of amplicon-specific fluorescent hydrolysis probes. Template that has been cleaved by Cas9 or another nuclease does not generate the first amplicon or activate the first probe. Uncleaved control DNA was digested with a restriction enzyme to create cleaved control DNA. Uncleaved and cleaved control DNA was mixed in specific proportions. (b) Representative droplet FAM and VIC probe intensities are shown for uncleaved control MYC locus template DNA (left panel), a 1:1 mixture of uncleaved:cleaved control DNA (middle panel), and cleaved control DNA (right panel). Colors indicate droplets with no template (gray), intact template (blue), and cleaved template (red). Droplet populations in the dot plots are: uncleaved control DNA (DSB: 120, no DSB: 1,724, no template: 12,360), 1:1 Mixture (DSB: 799, no DSB: 914, no template: 12,305), cleaved control DNA (DSB: 1,757, no DSB: 14, no template: 13,247).

    Article Snippet: The AAVS1 locus required addition of SmaI and AvrII restriction enzymes (New England Biolabs) to the master mix for better separation of target and template amplicon probe signals (see Bio-Rad instructions).

    Techniques: Polymerase Chain Reaction, Activation Assay, Amplification

    PCR-single-strand conformational polymorphism of the ovine IGF-1 gene. Amplicons were electrophoresed on a 12% non-denaturing acrylamide/bis-acrylamide gel; 200 V, 4 °C for 12 h. Three SSCP patterns, GG, AA and GA were detected.

    Journal: Journal of Genetic Engineering & Biotechnology

    Article Title: New polymorphism in the 5′ flanking region of IGF-1 gene and its association with wool traits in Egyptian Barki sheep

    doi: 10.1016/j.jgeb.2017.08.001

    Figure Lengend Snippet: PCR-single-strand conformational polymorphism of the ovine IGF-1 gene. Amplicons were electrophoresed on a 12% non-denaturing acrylamide/bis-acrylamide gel; 200 V, 4 °C for 12 h. Three SSCP patterns, GG, AA and GA were detected.

    Article Snippet: PCR amplicons were electrophoresed in 2% agarose gels, using 0.5X TBE buffer (89 mM Tris, 89 mM boric acid and 2 mM Na2 EDTA) containing 200 ng/ml of ethidium bromide and was visualized under UV light and photographed by Bio-Rad Laboratories, Hercules, CA, USA.

    Techniques: Polymerase Chain Reaction, Acrylamide Gel Assay