ampli taq gold dna polymerase  (Millipore)


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    Structured Review

    Millipore ampli taq gold dna polymerase
    Ampli Taq Gold Dna Polymerase, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ampli taq gold dna polymerase/product/Millipore
    Average 99 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    ampli taq gold dna polymerase - by Bioz Stars, 2020-07
    99/100 stars

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    Related Articles

    Negative Control:

    Article Title: SMAD2 Inactivation Inhibits CLDN6 Methylation to Suppress Migration and Invasion of Breast Cancer Cells
    Article Snippet: .. Antibodies included anti-DNMT1 (1:500, Cell Signaling Technology), anti-IgG (Millipore), and anti-DNA polymerase II (Millipore); anti-IgG was the negative control and anti-DNA polymerase II was the positive control. .. Transfection With Short Hairpin RNA Cells were transfected with short hairpin RNA (shRNA) by using Lipofectamine 2000 (Invitrogen), following the procedure recommended by the manufacturer. shRNA targeting of CLDN6 (5’-GTGCAAGGTGTACGACTCA-3’) and a negative control shRNA were purchased from GeneChem Co. Ltd.

    Amplification:

    Article Title: Molecular Characterization of Natural Erwinia pyrifoliae Strains Deficient in Hypersensitive Response
    Article Snippet: .. To reconfirm the hrpL sequences, DNA from strains Ep2/97, Ep4/97, and Ep16/96 was also amplified with a proofreading DNA polymerase (Accu Taq ; Sigma). .. The same sequence as in Fig. was obtained for the three wild-type strains, and the change from C to T at position 277 for strain Ep2/97 was observed.

    Positive Control:

    Article Title: SMAD2 Inactivation Inhibits CLDN6 Methylation to Suppress Migration and Invasion of Breast Cancer Cells
    Article Snippet: .. Antibodies included anti-DNMT1 (1:500, Cell Signaling Technology), anti-IgG (Millipore), and anti-DNA polymerase II (Millipore); anti-IgG was the negative control and anti-DNA polymerase II was the positive control. .. Transfection With Short Hairpin RNA Cells were transfected with short hairpin RNA (shRNA) by using Lipofectamine 2000 (Invitrogen), following the procedure recommended by the manufacturer. shRNA targeting of CLDN6 (5’-GTGCAAGGTGTACGACTCA-3’) and a negative control shRNA were purchased from GeneChem Co. Ltd.

    Activation Assay:

    Article Title: Transcriptomic profiling of human embryonic stem cells upon cell cycle manipulation during pluripotent state dissolution
    Article Snippet: .. 2.2 Experimental groups and conditions To understand the effects of DNA replication perturbation and activation of the DNA damage checkpoint in hESCs undergoing PSD, we supplemented hESCs either with DMSO, with the DNA polymerase inhibitor Aphidicolin (75 ng/mL, Sigma), or with Aphidicolin plus the checkpoint kinase inhibitor AZD7762 (100 nM, SelleckChem). .. To understand effects of Cyclin B1 overexpression in hESCs undergoing PSD, we infected hESCs either with the unmodified pLVTH vector or with the open reading frame of CCNB1 inserted using the PmeI and NdeI restriction sites.

    Labeling:

    Article Title: E2F1 Regulates the Base Excision Repair Gene XRCC1 and Promotes DNA Repair *
    Article Snippet: .. Cells were rinsed in phosphate-buffered saline, permeabilized with 1% Triton X-100 for 20 min, and then incubated in a moist air chamber at 37 °C for 90 min in a labeling mixture containing 10 μ m each dGTP, dATP, and dCTP and 7 μ m dTTP, 3 μ m FITC-dUTP, 20 units/ml Escherichia coli DNA polymerase I (Sigma) in reaction buffer containing 5 m m MgCl2 , 10 m m 2-mercaptoethanol, and 20 μg/ml bovine serum albumin. ..

    Concentration Assay:

    Article Title: The Heme Metabolite Carbon Monoxide Facilitates KSHV Infection by Inhibiting TLR4 Signaling in Endothelial Cells
    Article Snippet: .. The viral DNA polymerase inhibitor Foscarnet (FOS, Sigma) was used at a concentration of 400 μM. .. The CO releasing molecule CORM-2 (Sigma) was used at a concentration of 10 μM.

    Incubation:

    Article Title: E2F1 Regulates the Base Excision Repair Gene XRCC1 and Promotes DNA Repair *
    Article Snippet: .. Cells were rinsed in phosphate-buffered saline, permeabilized with 1% Triton X-100 for 20 min, and then incubated in a moist air chamber at 37 °C for 90 min in a labeling mixture containing 10 μ m each dGTP, dATP, and dCTP and 7 μ m dTTP, 3 μ m FITC-dUTP, 20 units/ml Escherichia coli DNA polymerase I (Sigma) in reaction buffer containing 5 m m MgCl2 , 10 m m 2-mercaptoethanol, and 20 μg/ml bovine serum albumin. ..

    Activity Assay:

    Article Title: Characterisation of Muta(TM)Mouse ?gt10-lacZ transgene: evidence for in vivo rearrangements
    Article Snippet: .. These custom-made primers (Cortec DNA Service Laboratories Inc., Kingston, Canada) were used to generate polymerase chain reaction (PCR) fragments in reactions involving a DNA polymerase deficient in 3′–5′ exonuclease activity (KOD polymerase; Novagen, Gibbstown, NJ, USA) to minimise proofreading errors. .. Fragments generated, either by PCR or cloning in E.coli , were sequenced in both directions using an ABI Prism® 3100 Genetic Analyzer and BigDye® Terminator Cycle Sequencing Kit (Applied Biosystems, Foster City, CA, USA).

    Polymerase Chain Reaction:

    Article Title: Characterisation of Muta(TM)Mouse ?gt10-lacZ transgene: evidence for in vivo rearrangements
    Article Snippet: .. These custom-made primers (Cortec DNA Service Laboratories Inc., Kingston, Canada) were used to generate polymerase chain reaction (PCR) fragments in reactions involving a DNA polymerase deficient in 3′–5′ exonuclease activity (KOD polymerase; Novagen, Gibbstown, NJ, USA) to minimise proofreading errors. .. Fragments generated, either by PCR or cloning in E.coli , were sequenced in both directions using an ABI Prism® 3100 Genetic Analyzer and BigDye® Terminator Cycle Sequencing Kit (Applied Biosystems, Foster City, CA, USA).

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    Millipore one step rt pcr master mix kit
    Amplification curves of 10-fold dilutions of the RVA strain Wa dsRNA transcript spiked with MS2 bacteriophage RNA (from 6.5 × 10 6 to 6.5 copies per reaction), obtained with the <t>EMD</t> Millipore NSP3 <t>qRT-PCR</t> assay. Using the threshold for delta Rn (the normalized reporter value [Rn] of the reaction minus the Rn of the baseline signal) (green line), black curves show the 10-fold dilutions of the NSP3 gene transcript and red curves show MS2 IPC amplification. The graph showing the C T value versus the log copy number was fitted with a regression line, and the slope for calculation of efficiency was obtained from the regression line. The fluorescent signals from RVA-negative samples and no-template controls are indicated by the blue outline.
    One Step Rt Pcr Master Mix Kit, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/one step rt pcr master mix kit/product/Millipore
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    one step rt pcr master mix kit - by Bioz Stars, 2020-07
    99/100 stars
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    99
    Millipore pcr
    Amplification curves of 10-fold dilutions of the RVA strain Wa dsRNA transcript spiked with MS2 bacteriophage RNA (from 6.5 × 10 6 to 6.5 copies per reaction), obtained with the <t>EMD</t> Millipore NSP3 <t>qRT-PCR</t> assay. Using the threshold for delta Rn (the normalized reporter value [Rn] of the reaction minus the Rn of the baseline signal) (green line), black curves show the 10-fold dilutions of the NSP3 gene transcript and red curves show MS2 IPC amplification. The graph showing the C T value versus the log copy number was fitted with a regression line, and the slope for calculation of efficiency was obtained from the regression line. The fluorescent signals from RVA-negative samples and no-template controls are indicated by the blue outline.
    Pcr, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 11792 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcr/product/Millipore
    Average 99 stars, based on 11792 article reviews
    Price from $9.99 to $1999.99
    pcr - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

    97
    Millipore terminal deoxynucleotidyl transferase tdt mediated deoxyuridine triphosphate dutp nick end labeling tunel assay
    Amplification curves of 10-fold dilutions of the RVA strain Wa dsRNA transcript spiked with MS2 bacteriophage RNA (from 6.5 × 10 6 to 6.5 copies per reaction), obtained with the <t>EMD</t> Millipore NSP3 <t>qRT-PCR</t> assay. Using the threshold for delta Rn (the normalized reporter value [Rn] of the reaction minus the Rn of the baseline signal) (green line), black curves show the 10-fold dilutions of the NSP3 gene transcript and red curves show MS2 IPC amplification. The graph showing the C T value versus the log copy number was fitted with a regression line, and the slope for calculation of efficiency was obtained from the regression line. The fluorescent signals from RVA-negative samples and no-template controls are indicated by the blue outline.
    Terminal Deoxynucleotidyl Transferase Tdt Mediated Deoxyuridine Triphosphate Dutp Nick End Labeling Tunel Assay, supplied by Millipore, used in various techniques. Bioz Stars score: 97/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/terminal deoxynucleotidyl transferase tdt mediated deoxyuridine triphosphate dutp nick end labeling tunel assay/product/Millipore
    Average 97 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    terminal deoxynucleotidyl transferase tdt mediated deoxyuridine triphosphate dutp nick end labeling tunel assay - by Bioz Stars, 2020-07
    97/100 stars
      Buy from Supplier

    Image Search Results


    Amplification curves of 10-fold dilutions of the RVA strain Wa dsRNA transcript spiked with MS2 bacteriophage RNA (from 6.5 × 10 6 to 6.5 copies per reaction), obtained with the EMD Millipore NSP3 qRT-PCR assay. Using the threshold for delta Rn (the normalized reporter value [Rn] of the reaction minus the Rn of the baseline signal) (green line), black curves show the 10-fold dilutions of the NSP3 gene transcript and red curves show MS2 IPC amplification. The graph showing the C T value versus the log copy number was fitted with a regression line, and the slope for calculation of efficiency was obtained from the regression line. The fluorescent signals from RVA-negative samples and no-template controls are indicated by the blue outline.

    Journal: Journal of Clinical Microbiology

    Article Title: Evaluation of an Alternative Recombinant Thermostable Thermus thermophilus (rTth)-Based Real-Time Reverse Transcription-PCR Kit for Detection of Rotavirus A

    doi: 10.1128/JCM.00126-17

    Figure Lengend Snippet: Amplification curves of 10-fold dilutions of the RVA strain Wa dsRNA transcript spiked with MS2 bacteriophage RNA (from 6.5 × 10 6 to 6.5 copies per reaction), obtained with the EMD Millipore NSP3 qRT-PCR assay. Using the threshold for delta Rn (the normalized reporter value [Rn] of the reaction minus the Rn of the baseline signal) (green line), black curves show the 10-fold dilutions of the NSP3 gene transcript and red curves show MS2 IPC amplification. The graph showing the C T value versus the log copy number was fitted with a regression line, and the slope for calculation of efficiency was obtained from the regression line. The fluorescent signals from RVA-negative samples and no-template controls are indicated by the blue outline.

    Article Snippet: Here, we report evaluation of a one-step RT-PCR master mix kit (EMD Millipore Corporation, Billerica, MA, USA) for detection of the RVA NSP3 gene.

    Techniques: Amplification, Quantitative RT-PCR