Structured Review

Millipore ampk specific inhibitor
Experimental design and animal groups. DHE: dihydroethidium; DMSO: dimethyl sulfoxide; <t>dorsomorphin:</t> <t>AMPK</t> inhibitor; Eze: Ezetimibe; IHC: immunohistochemistry; MCAO: middle cerebral artery occlusion; MDA: malonaldehyde; Scr siRNA: scramble siRNA; TTC: 2,3,5-triphenyltetrazolium chloride; WB: western blot.
Ampk Specific Inhibitor, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ampk specific inhibitor/product/Millipore
Average 99 stars, based on 1 article reviews
Price from $9.99 to $1999.99
ampk specific inhibitor - by Bioz Stars, 2021-09
99/100 stars

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1) Product Images from "Ezetimibe Attenuates Oxidative Stress and Neuroinflammation via the AMPK/Nrf2/TXNIP Pathway after MCAO in Rats"

Article Title: Ezetimibe Attenuates Oxidative Stress and Neuroinflammation via the AMPK/Nrf2/TXNIP Pathway after MCAO in Rats

Journal: Oxidative Medicine and Cellular Longevity

doi: 10.1155/2020/4717258

Experimental design and animal groups. DHE: dihydroethidium; DMSO: dimethyl sulfoxide; dorsomorphin: AMPK inhibitor; Eze: Ezetimibe; IHC: immunohistochemistry; MCAO: middle cerebral artery occlusion; MDA: malonaldehyde; Scr siRNA: scramble siRNA; TTC: 2,3,5-triphenyltetrazolium chloride; WB: western blot.
Figure Legend Snippet: Experimental design and animal groups. DHE: dihydroethidium; DMSO: dimethyl sulfoxide; dorsomorphin: AMPK inhibitor; Eze: Ezetimibe; IHC: immunohistochemistry; MCAO: middle cerebral artery occlusion; MDA: malonaldehyde; Scr siRNA: scramble siRNA; TTC: 2,3,5-triphenyltetrazolium chloride; WB: western blot.

Techniques Used: Immunohistochemistry, Multiple Displacement Amplification, Western Blot

Related Articles

other:

Article Title: Metformin Reduces Lipotoxicity-Induced Meta-Inflammation in β-Cells through the Activation of GPR40-PLC-IP3 Pathway
Article Snippet: AMPK Inhibitor and Agonist Treatment β TC6 cells were pretreated with 10 μ mol/L AMPK inhibitor (compound C; Sigma-Aldrich, USA) [ ] for 4 h before being exposed to 0.5 mmol/L PA+DMEM (25 mmol/L glucose)+100 μ mol/L MF for 72 h. Cells exposed to compound C or complete medium alone were used as controls. β TC6 cells were pretreated with 0.5 mmol/L PA+DMEM (25 mmol/L glucose) for 24 h. Consequently, cells were treated with 1 mmol/L of AMPK agonist AICAR (dissolved in 0.1% DMSO (v /v ); Henan DaKen Chemical CO., LTD.) [ ] for additional 72 h. PA-induced cells+DMEM (25 mmol/L glucose) without AICAR and uninduced cells exposed to AICAR served as controls.

Article Title: Quercetin-induced apoptosis ameliorates vascular smooth muscle cell senescence through AMP-activated protein kinase signaling pathway
Article Snippet: Compound C, an AMPK inhibitor, was provided by Calbiochem (La Jolla, CA, USA).

Activation Assay:

Article Title: Anabolic SIRT4 Exerts Retrograde Control over TORC1 Signaling by Glutamine Sparing in the Mitochondria
Article Snippet: .. For AMPK activation, 0.5 mM AICAR (Sigma, catalog no. A9978) was used as a high dose, and 0.03 mM was used as a low dose for 1 h. For AMPK inhibition, compound C (dorsomorphin; Sigma, catalog no. A5499) was used at a 20 μM dose for 1 h. For increasing anaplerotic flux, 10 mM dimethyl α-ketoglutarate (DMKG; Sigma, catalog no. 349631) was added to cells for 1 h. For Arf1 inhibition, 10 μM brefeldin A was added in low-glucose conditions for 1 h. For inhibition of autophagy flux, the cells were kept in either low (5 mM)- or high (25 mM)-glucose medium with or without 100 μM leupeptin (Sigma, catalog no. L2884) for 12 h. For the qPCR and luciferase assays, the cells were kept in the indicated medium conditions for 6 h. ..

Inhibition:

Article Title: Anabolic SIRT4 Exerts Retrograde Control over TORC1 Signaling by Glutamine Sparing in the Mitochondria
Article Snippet: .. For AMPK activation, 0.5 mM AICAR (Sigma, catalog no. A9978) was used as a high dose, and 0.03 mM was used as a low dose for 1 h. For AMPK inhibition, compound C (dorsomorphin; Sigma, catalog no. A5499) was used at a 20 μM dose for 1 h. For increasing anaplerotic flux, 10 mM dimethyl α-ketoglutarate (DMKG; Sigma, catalog no. 349631) was added to cells for 1 h. For Arf1 inhibition, 10 μM brefeldin A was added in low-glucose conditions for 1 h. For inhibition of autophagy flux, the cells were kept in either low (5 mM)- or high (25 mM)-glucose medium with or without 100 μM leupeptin (Sigma, catalog no. L2884) for 12 h. For the qPCR and luciferase assays, the cells were kept in the indicated medium conditions for 6 h. ..

Real-time Polymerase Chain Reaction:

Article Title: Anabolic SIRT4 Exerts Retrograde Control over TORC1 Signaling by Glutamine Sparing in the Mitochondria
Article Snippet: .. For AMPK activation, 0.5 mM AICAR (Sigma, catalog no. A9978) was used as a high dose, and 0.03 mM was used as a low dose for 1 h. For AMPK inhibition, compound C (dorsomorphin; Sigma, catalog no. A5499) was used at a 20 μM dose for 1 h. For increasing anaplerotic flux, 10 mM dimethyl α-ketoglutarate (DMKG; Sigma, catalog no. 349631) was added to cells for 1 h. For Arf1 inhibition, 10 μM brefeldin A was added in low-glucose conditions for 1 h. For inhibition of autophagy flux, the cells were kept in either low (5 mM)- or high (25 mM)-glucose medium with or without 100 μM leupeptin (Sigma, catalog no. L2884) for 12 h. For the qPCR and luciferase assays, the cells were kept in the indicated medium conditions for 6 h. ..

Luciferase:

Article Title: Anabolic SIRT4 Exerts Retrograde Control over TORC1 Signaling by Glutamine Sparing in the Mitochondria
Article Snippet: .. For AMPK activation, 0.5 mM AICAR (Sigma, catalog no. A9978) was used as a high dose, and 0.03 mM was used as a low dose for 1 h. For AMPK inhibition, compound C (dorsomorphin; Sigma, catalog no. A5499) was used at a 20 μM dose for 1 h. For increasing anaplerotic flux, 10 mM dimethyl α-ketoglutarate (DMKG; Sigma, catalog no. 349631) was added to cells for 1 h. For Arf1 inhibition, 10 μM brefeldin A was added in low-glucose conditions for 1 h. For inhibition of autophagy flux, the cells were kept in either low (5 mM)- or high (25 mM)-glucose medium with or without 100 μM leupeptin (Sigma, catalog no. L2884) for 12 h. For the qPCR and luciferase assays, the cells were kept in the indicated medium conditions for 6 h. ..

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  • 99
    Millipore ampk specific inhibitor
    Experimental design and animal groups. DHE: dihydroethidium; DMSO: dimethyl sulfoxide; <t>dorsomorphin:</t> <t>AMPK</t> inhibitor; Eze: Ezetimibe; IHC: immunohistochemistry; MCAO: middle cerebral artery occlusion; MDA: malonaldehyde; Scr siRNA: scramble siRNA; TTC: 2,3,5-triphenyltetrazolium chloride; WB: western blot.
    Ampk Specific Inhibitor, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ampk specific inhibitor/product/Millipore
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ampk specific inhibitor - by Bioz Stars, 2021-09
    99/100 stars
      Buy from Supplier

    93
    Millipore ampk inhibitor 5 iodotubercidin
    The protective effect of metformin involves the PI3-kinase/Akt pathway, but not the <t>AMPK/mTOR</t> pathway. (A) Primary rat hepatocytes were exposed to GCDCA (50 µmol/L) and metformin (1 mmol/L) for 4 hrs with or without <t>5′-iodotubercidin</t> (0.1 µmol/L), an inhibitor of AMP-activated protein kinase, and the mTOR inhibitor rapamycin (0.5 µmol/L). Caspase-3 like activity is presented as fold induction compared to control values; control values were set at one. Data are presented as mean of at least three independent experiments +/−S.E.M. Statistical analysis: * p
    Ampk Inhibitor 5 Iodotubercidin, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ampk inhibitor 5 iodotubercidin/product/Millipore
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ampk inhibitor 5 iodotubercidin - by Bioz Stars, 2021-09
    93/100 stars
      Buy from Supplier

    Image Search Results


    Experimental design and animal groups. DHE: dihydroethidium; DMSO: dimethyl sulfoxide; dorsomorphin: AMPK inhibitor; Eze: Ezetimibe; IHC: immunohistochemistry; MCAO: middle cerebral artery occlusion; MDA: malonaldehyde; Scr siRNA: scramble siRNA; TTC: 2,3,5-triphenyltetrazolium chloride; WB: western blot.

    Journal: Oxidative Medicine and Cellular Longevity

    Article Title: Ezetimibe Attenuates Oxidative Stress and Neuroinflammation via the AMPK/Nrf2/TXNIP Pathway after MCAO in Rats

    doi: 10.1155/2020/4717258

    Figure Lengend Snippet: Experimental design and animal groups. DHE: dihydroethidium; DMSO: dimethyl sulfoxide; dorsomorphin: AMPK inhibitor; Eze: Ezetimibe; IHC: immunohistochemistry; MCAO: middle cerebral artery occlusion; MDA: malonaldehyde; Scr siRNA: scramble siRNA; TTC: 2,3,5-triphenyltetrazolium chloride; WB: western blot.

    Article Snippet: The AMPK-specific inhibitor, dorsomorphin (0.1 μ mol, purity ≥ 98%, P5499, Sigma-Aldrich, St. Louis, MO, USA), was dissolved in 20% DMSO in PBS, and 10 μ l was delivered into the ipsilateral ventricle with a Hamilton syringe (Microliter 701; Hamilton Company, USA) 30 min before MCAO [ ].

    Techniques: Immunohistochemistry, Multiple Displacement Amplification, Western Blot

    Med1-inducible liver cell proliferation is attenuated by AMPK inhibitor compound C. A and B , wild-type mice ( A ) and Ad-LacZ-injected mice ( B ) were given BrdUrd in drinking water for 3 days. C and D , mice were infected with Ad-Med1 by tail vein injection and given intraperitoneal injections of vehicle ( C ) or compound C ( D ) daily for 3 days with BrdUrd in drinking water. The livers of mice were processed for BrdUrd incorporation. E , quantification of BrdUrd-labeled nuclei shown in A–D . Nuclear labeling of hepatocytes indicates attenuation of Med-inducible hepatocyte proliferation by compound C.

    Journal: The Journal of Biological Chemistry

    Article Title: The Med1 Subunit of the Mediator Complex Induces Liver Cell Proliferation and Is Phosphorylated by AMP Kinase *

    doi: 10.1074/jbc.M113.486696

    Figure Lengend Snippet: Med1-inducible liver cell proliferation is attenuated by AMPK inhibitor compound C. A and B , wild-type mice ( A ) and Ad-LacZ-injected mice ( B ) were given BrdUrd in drinking water for 3 days. C and D , mice were infected with Ad-Med1 by tail vein injection and given intraperitoneal injections of vehicle ( C ) or compound C ( D ) daily for 3 days with BrdUrd in drinking water. The livers of mice were processed for BrdUrd incorporation. E , quantification of BrdUrd-labeled nuclei shown in A–D . Nuclear labeling of hepatocytes indicates attenuation of Med-inducible hepatocyte proliferation by compound C.

    Article Snippet: The AMPK activator AICAR was purchased from Tocris Bioscience (Minneapolis, MN), and the specific inhibitor compound C and fenofibrate were obtained from Sigma.

    Techniques: Mouse Assay, Injection, Infection, Labeling

    The protective effect of metformin involves the PI3-kinase/Akt pathway, but not the AMPK/mTOR pathway. (A) Primary rat hepatocytes were exposed to GCDCA (50 µmol/L) and metformin (1 mmol/L) for 4 hrs with or without 5′-iodotubercidin (0.1 µmol/L), an inhibitor of AMP-activated protein kinase, and the mTOR inhibitor rapamycin (0.5 µmol/L). Caspase-3 like activity is presented as fold induction compared to control values; control values were set at one. Data are presented as mean of at least three independent experiments +/−S.E.M. Statistical analysis: * p

    Journal: PLoS ONE

    Article Title: Metformin Protects Rat Hepatocytes against Bile Acid-Induced Apoptosis

    doi: 10.1371/journal.pone.0071773

    Figure Lengend Snippet: The protective effect of metformin involves the PI3-kinase/Akt pathway, but not the AMPK/mTOR pathway. (A) Primary rat hepatocytes were exposed to GCDCA (50 µmol/L) and metformin (1 mmol/L) for 4 hrs with or without 5′-iodotubercidin (0.1 µmol/L), an inhibitor of AMP-activated protein kinase, and the mTOR inhibitor rapamycin (0.5 µmol/L). Caspase-3 like activity is presented as fold induction compared to control values; control values were set at one. Data are presented as mean of at least three independent experiments +/−S.E.M. Statistical analysis: * p

    Article Snippet: Signal transduction pathways were specifically blocked using 0.1 µmol/L of the AMPK inhibitor 5′-iodotubercidin (Calbiochem), 0.5 µmol/L of the mTOR inhibitor rapamycin (Calbiochem) or 50 µmol/L of the PI3K inhibitor LY 294002 (Calbiochem).

    Techniques: Activity Assay