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Stratagene ampicillin resistance gene
Ampicillin Resistance Gene, supplied by Stratagene, used in various techniques. Bioz Stars score: 90/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ampicillin resistance gene/product/Stratagene
Average 90 stars, based on 10 article reviews
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ampicillin resistance gene - by Bioz Stars, 2020-09
90/100 stars

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Related Articles

Clone Assay:

Article Title: Four-Dimensional Visualization of the Simultaneous Activity of Alternative Adeno-Associated Virus Replication Origins †
Article Snippet: .. Expression of rep68/rep78 from a kanamycin-resistant plasmid was required, because in the replication assay (see Fig. ), replication products of pBs-p5-tetO were detected with a probe for the ampicillin resistance gene. (iv) For pBs-p5-tetO, a 330-bp SalI fragment (containing seven tetracycline repressor [TetR] binding sites) from pSA1.TetO.EYFPnlsTetR ( ) was self-ligated, and a five-copy repeat was inserted into the unique XhoI site of the pBluescript II KS(+) cloning vector (Stratagene), resulting in plasmid pBstetO. .. A fragment containing the AAV p5 promoter (AAV2 nt 152 to 299) was amplified by PCR from plasmid pAV2 ( ) using primers p5ABforward (5′AAAATT ACTAGT GGAGTCGTGACGTGAATTA3′) and p5Dgreverse (5′AAAATT GCGGCCGC CCGCTTCAAAATGGAGA3′), which were designed to introduce a SpeI and a NotI site (underlined), respectively.

Article Title: A Novel Role for Escherichia coli Endonuclease VIII in Prevention of Spontaneous G- > T Transversions
Article Snippet: .. A cassette carrying the ampicillin resistance gene was PCR amplified from the phagemid pBluescript II (Stratagene) and cloned in place of fpg in the above-mentioned construct. ..

Amplification:

Article Title: Inhibition of trypsin expression in Lutzomyia longipalpis using RNAi enhances the survival of Leishmania
Article Snippet: .. Double stranded RNA synthesis The trypsin 1 template was PCR amplified based on the Lu. longipalpis trypsin 1 gene sequence described before (GenBank accession number EF011106 ; ref 16) using a plasmid preparation obtained from a normalized Lu. longipalpis cDNA Library [ ], and the ampicillin resistance gene (AMP; used as a control) was PCR amplified from the pBluescript SK plasmid (Stratagene). .. PCR was carried out using specific primers containing the T7 site at their 5 prime ends (Table ).

Article Title: A Novel Role for Escherichia coli Endonuclease VIII in Prevention of Spontaneous G- > T Transversions
Article Snippet: .. A cassette carrying the ampicillin resistance gene was PCR amplified from the phagemid pBluescript II (Stratagene) and cloned in place of fpg in the above-mentioned construct. ..

Southern Blot:

Article Title: Salmonella enterica Serovar Enteritidis, Japan
Article Snippet: .. Southern blot analysis by using the ampicillin resistance gene of pBluescript KS (+) (Stratagene, La Jolla, CA) as a probe indicated that a resistance gene was carried on the 50-kb plasmid. .. Furthermore, when Escherichia coli DH10B cells (Invitrogen Corporation, Carlsbad, CA) were transformed with plasmids isolated from an RDNC-a R-AS strain and plated onto Luria broth plates containing 100 mg/L of ampicillin, the 50-kb, but not 60-kb, plasmid could be isolated from the ampicillin-resistant transformants.

Construct:

Article Title: A Novel Role for Escherichia coli Endonuclease VIII in Prevention of Spontaneous G- > T Transversions
Article Snippet: .. A cassette carrying the ampicillin resistance gene was PCR amplified from the phagemid pBluescript II (Stratagene) and cloned in place of fpg in the above-mentioned construct. ..

Polymerase Chain Reaction:

Article Title: Inhibition of trypsin expression in Lutzomyia longipalpis using RNAi enhances the survival of Leishmania
Article Snippet: .. Double stranded RNA synthesis The trypsin 1 template was PCR amplified based on the Lu. longipalpis trypsin 1 gene sequence described before (GenBank accession number EF011106 ; ref 16) using a plasmid preparation obtained from a normalized Lu. longipalpis cDNA Library [ ], and the ampicillin resistance gene (AMP; used as a control) was PCR amplified from the pBluescript SK plasmid (Stratagene). .. PCR was carried out using specific primers containing the T7 site at their 5 prime ends (Table ).

Article Title: A Novel Role for Escherichia coli Endonuclease VIII in Prevention of Spontaneous G- > T Transversions
Article Snippet: .. A cassette carrying the ampicillin resistance gene was PCR amplified from the phagemid pBluescript II (Stratagene) and cloned in place of fpg in the above-mentioned construct. ..

Generated:

Article Title: A Novel Selection Marker for Efficient DNA Cloning and Recombineering in E. coli
Article Snippet: .. Vector pF was generated by replacing the ampicillin resistance gene in pBluescript (pBS) (Stratagene) with fabI . .. By PCR mutagenesis, the G93V point mutation was introduced into the fabI in pF, resulting in mfabI , to generate pF2 ( ).

cDNA Library Assay:

Article Title: Inhibition of trypsin expression in Lutzomyia longipalpis using RNAi enhances the survival of Leishmania
Article Snippet: .. Double stranded RNA synthesis The trypsin 1 template was PCR amplified based on the Lu. longipalpis trypsin 1 gene sequence described before (GenBank accession number EF011106 ; ref 16) using a plasmid preparation obtained from a normalized Lu. longipalpis cDNA Library [ ], and the ampicillin resistance gene (AMP; used as a control) was PCR amplified from the pBluescript SK plasmid (Stratagene). .. PCR was carried out using specific primers containing the T7 site at their 5 prime ends (Table ).

Expressing:

Article Title: Four-Dimensional Visualization of the Simultaneous Activity of Alternative Adeno-Associated Virus Replication Origins †
Article Snippet: .. Expression of rep68/rep78 from a kanamycin-resistant plasmid was required, because in the replication assay (see Fig. ), replication products of pBs-p5-tetO were detected with a probe for the ampicillin resistance gene. (iv) For pBs-p5-tetO, a 330-bp SalI fragment (containing seven tetracycline repressor [TetR] binding sites) from pSA1.TetO.EYFPnlsTetR ( ) was self-ligated, and a five-copy repeat was inserted into the unique XhoI site of the pBluescript II KS(+) cloning vector (Stratagene), resulting in plasmid pBstetO. .. A fragment containing the AAV p5 promoter (AAV2 nt 152 to 299) was amplified by PCR from plasmid pAV2 ( ) using primers p5ABforward (5′AAAATT ACTAGT GGAGTCGTGACGTGAATTA3′) and p5Dgreverse (5′AAAATT GCGGCCGC CCGCTTCAAAATGGAGA3′), which were designed to introduce a SpeI and a NotI site (underlined), respectively.

Modification:

Article Title: Generation, affinity maturation, and characterization of a human anti-human NKG2D monoclonal antibody with dual antagonistic and agonistic activity
Article Snippet: .. For the first step, a modified pC3C phagemid, pC3C-Cam, was used in which the ampicillin resistance gene was replaced by the chloramphenicol resistance gene from plasmid pPCR-Script Cam SK(+) (Stratagene). .. The previously amplified Vκ and Vλ encoding sequences from all 6 donors were combined with the VH encoding sequence of KYK-1.0 through Vκ -Cκ -VH and Vλ -Cλ -VH cassette assembly as described for the generation of the naïve human Fab library, digested with Sfi I, and cloned into pC3C-Cam.

Sequencing:

Article Title: Inhibition of trypsin expression in Lutzomyia longipalpis using RNAi enhances the survival of Leishmania
Article Snippet: .. Double stranded RNA synthesis The trypsin 1 template was PCR amplified based on the Lu. longipalpis trypsin 1 gene sequence described before (GenBank accession number EF011106 ; ref 16) using a plasmid preparation obtained from a normalized Lu. longipalpis cDNA Library [ ], and the ampicillin resistance gene (AMP; used as a control) was PCR amplified from the pBluescript SK plasmid (Stratagene). .. PCR was carried out using specific primers containing the T7 site at their 5 prime ends (Table ).

Binding Assay:

Article Title: Four-Dimensional Visualization of the Simultaneous Activity of Alternative Adeno-Associated Virus Replication Origins †
Article Snippet: .. Expression of rep68/rep78 from a kanamycin-resistant plasmid was required, because in the replication assay (see Fig. ), replication products of pBs-p5-tetO were detected with a probe for the ampicillin resistance gene. (iv) For pBs-p5-tetO, a 330-bp SalI fragment (containing seven tetracycline repressor [TetR] binding sites) from pSA1.TetO.EYFPnlsTetR ( ) was self-ligated, and a five-copy repeat was inserted into the unique XhoI site of the pBluescript II KS(+) cloning vector (Stratagene), resulting in plasmid pBstetO. .. A fragment containing the AAV p5 promoter (AAV2 nt 152 to 299) was amplified by PCR from plasmid pAV2 ( ) using primers p5ABforward (5′AAAATT ACTAGT GGAGTCGTGACGTGAATTA3′) and p5Dgreverse (5′AAAATT GCGGCCGC CCGCTTCAAAATGGAGA3′), which were designed to introduce a SpeI and a NotI site (underlined), respectively.

Chick Chorioallantoic Membrane Assay:

Article Title: Generation, affinity maturation, and characterization of a human anti-human NKG2D monoclonal antibody with dual antagonistic and agonistic activity
Article Snippet: .. For the first step, a modified pC3C phagemid, pC3C-Cam, was used in which the ampicillin resistance gene was replaced by the chloramphenicol resistance gene from plasmid pPCR-Script Cam SK(+) (Stratagene). .. The previously amplified Vκ and Vλ encoding sequences from all 6 donors were combined with the VH encoding sequence of KYK-1.0 through Vκ -Cκ -VH and Vλ -Cλ -VH cassette assembly as described for the generation of the naïve human Fab library, digested with Sfi I, and cloned into pC3C-Cam.

Plasmid Preparation:

Article Title: Salmonella enterica Serovar Enteritidis, Japan
Article Snippet: .. Southern blot analysis by using the ampicillin resistance gene of pBluescript KS (+) (Stratagene, La Jolla, CA) as a probe indicated that a resistance gene was carried on the 50-kb plasmid. .. Furthermore, when Escherichia coli DH10B cells (Invitrogen Corporation, Carlsbad, CA) were transformed with plasmids isolated from an RDNC-a R-AS strain and plated onto Luria broth plates containing 100 mg/L of ampicillin, the 50-kb, but not 60-kb, plasmid could be isolated from the ampicillin-resistant transformants.

Article Title: A Novel Selection Marker for Efficient DNA Cloning and Recombineering in E. coli
Article Snippet: .. Vector pF was generated by replacing the ampicillin resistance gene in pBluescript (pBS) (Stratagene) with fabI . .. By PCR mutagenesis, the G93V point mutation was introduced into the fabI in pF, resulting in mfabI , to generate pF2 ( ).

Article Title: Four-Dimensional Visualization of the Simultaneous Activity of Alternative Adeno-Associated Virus Replication Origins †
Article Snippet: .. Expression of rep68/rep78 from a kanamycin-resistant plasmid was required, because in the replication assay (see Fig. ), replication products of pBs-p5-tetO were detected with a probe for the ampicillin resistance gene. (iv) For pBs-p5-tetO, a 330-bp SalI fragment (containing seven tetracycline repressor [TetR] binding sites) from pSA1.TetO.EYFPnlsTetR ( ) was self-ligated, and a five-copy repeat was inserted into the unique XhoI site of the pBluescript II KS(+) cloning vector (Stratagene), resulting in plasmid pBstetO. .. A fragment containing the AAV p5 promoter (AAV2 nt 152 to 299) was amplified by PCR from plasmid pAV2 ( ) using primers p5ABforward (5′AAAATT ACTAGT GGAGTCGTGACGTGAATTA3′) and p5Dgreverse (5′AAAATT GCGGCCGC CCGCTTCAAAATGGAGA3′), which were designed to introduce a SpeI and a NotI site (underlined), respectively.

Article Title: Generation, affinity maturation, and characterization of a human anti-human NKG2D monoclonal antibody with dual antagonistic and agonistic activity
Article Snippet: .. For the first step, a modified pC3C phagemid, pC3C-Cam, was used in which the ampicillin resistance gene was replaced by the chloramphenicol resistance gene from plasmid pPCR-Script Cam SK(+) (Stratagene). .. The previously amplified Vκ and Vλ encoding sequences from all 6 donors were combined with the VH encoding sequence of KYK-1.0 through Vκ -Cκ -VH and Vλ -Cλ -VH cassette assembly as described for the generation of the naïve human Fab library, digested with Sfi I, and cloned into pC3C-Cam.

Article Title: Inhibition of trypsin expression in Lutzomyia longipalpis using RNAi enhances the survival of Leishmania
Article Snippet: .. Double stranded RNA synthesis The trypsin 1 template was PCR amplified based on the Lu. longipalpis trypsin 1 gene sequence described before (GenBank accession number EF011106 ; ref 16) using a plasmid preparation obtained from a normalized Lu. longipalpis cDNA Library [ ], and the ampicillin resistance gene (AMP; used as a control) was PCR amplified from the pBluescript SK plasmid (Stratagene). .. PCR was carried out using specific primers containing the T7 site at their 5 prime ends (Table ).

Article Title: Expression of phosphofructokinase in Neisseria meningitidis
Article Snippet: .. First, the pTOPO- pfkA plasmid and a pBluescript vector, which contains an ampicillin resistance gene (Stratagene), were digested by Hin dIII and Eco RV. .. Digestion of the pTOPO- pfkA by these enzymes resulted in an 1173 bp fragment containing pfkA which was purified as described above.

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    Stratagene ampicillin resistance gene amp
    Kaplan-Meier plots showing survival of Lu. <t>longipalpis</t> under different nutritional conditions (a) Representative plot of sand flies bloodfed 96h after injection with XDH or <t>AMP</t> dsRNA where the mortality of an initial group of 34 (dsXDH) and 50 (dsAMP) sand flies was monitored daily. (b) Representative plot of flies injected with XDH and AMP dsRNA and sugar fed after injections. An initial group of 90 (dsXDH) and 84 (dsAMP) sand flies were monitored daily for mortality. Statistical analysis was based on at least two independent experiments.
    Ampicillin Resistance Gene Amp, supplied by Stratagene, used in various techniques. Bioz Stars score: 85/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ampicillin resistance gene amp/product/Stratagene
    Average 85 stars, based on 11 article reviews
    Price from $9.99 to $1999.99
    ampicillin resistance gene amp - by Bioz Stars, 2020-09
    85/100 stars
      Buy from Supplier

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    Kaplan-Meier plots showing survival of Lu. longipalpis under different nutritional conditions (a) Representative plot of sand flies bloodfed 96h after injection with XDH or AMP dsRNA where the mortality of an initial group of 34 (dsXDH) and 50 (dsAMP) sand flies was monitored daily. (b) Representative plot of flies injected with XDH and AMP dsRNA and sugar fed after injections. An initial group of 90 (dsXDH) and 84 (dsAMP) sand flies were monitored daily for mortality. Statistical analysis was based on at least two independent experiments.

    Journal: Insect biochemistry and molecular biology

    Article Title: Gene silencing in Phlebotomine sand flies: xanthine dehydrogenase knock down by dsRNA micro-injections

    doi: 10.1016/j.ibmb.2008.03.012

    Figure Lengend Snippet: Kaplan-Meier plots showing survival of Lu. longipalpis under different nutritional conditions (a) Representative plot of sand flies bloodfed 96h after injection with XDH or AMP dsRNA where the mortality of an initial group of 34 (dsXDH) and 50 (dsAMP) sand flies was monitored daily. (b) Representative plot of flies injected with XDH and AMP dsRNA and sugar fed after injections. An initial group of 90 (dsXDH) and 84 (dsAMP) sand flies were monitored daily for mortality. Statistical analysis was based on at least two independent experiments.

    Article Snippet: The XDH template was PCR amplified from the XDH gene plasmid preparation obtained from a normalized Lu. longipalpis cDNA Library ( ) and the ampicillin resistance gene (AMP) was PCR amplified from the pBluescript SK plasmid (Stratagene).

    Techniques: Injection

    RT-PCR verification of xanthine dehydrogenase (XDH) expression from Lu. longipalpis injected with XDH and ampicillin (AMP) dsRNAs. RT-PCR of the ribosomal 60S gene was used as loading control. (a) Reduction in XDH expression in sand flies injected with XDH dsRNA (Lanes 1, 2 and 3) compared to flies injected with AMP dsRNA (lines 4, 5 and 6). Lanes 1, 2, 4 and 5 represent XDH expression from 1 sand fly and lanes 3 and 6 represent XDH expression of a pool of 20 sand flies. ( b ) 3 independent experiments were done and relative XDH gene expression was expressed by the ratio between band densitometry values in an RT-PCR product of the XDH gene and the control Ribosomal 60S gene for dsXDH and dsAMP-injected sand flies. Individual insects, as well as pools of 20 insects were used in each experimental groups. Asterisk represents significant statistical significance at p

    Journal: Insect biochemistry and molecular biology

    Article Title: Gene silencing in Phlebotomine sand flies: xanthine dehydrogenase knock down by dsRNA micro-injections

    doi: 10.1016/j.ibmb.2008.03.012

    Figure Lengend Snippet: RT-PCR verification of xanthine dehydrogenase (XDH) expression from Lu. longipalpis injected with XDH and ampicillin (AMP) dsRNAs. RT-PCR of the ribosomal 60S gene was used as loading control. (a) Reduction in XDH expression in sand flies injected with XDH dsRNA (Lanes 1, 2 and 3) compared to flies injected with AMP dsRNA (lines 4, 5 and 6). Lanes 1, 2, 4 and 5 represent XDH expression from 1 sand fly and lanes 3 and 6 represent XDH expression of a pool of 20 sand flies. ( b ) 3 independent experiments were done and relative XDH gene expression was expressed by the ratio between band densitometry values in an RT-PCR product of the XDH gene and the control Ribosomal 60S gene for dsXDH and dsAMP-injected sand flies. Individual insects, as well as pools of 20 insects were used in each experimental groups. Asterisk represents significant statistical significance at p

    Article Snippet: The XDH template was PCR amplified from the XDH gene plasmid preparation obtained from a normalized Lu. longipalpis cDNA Library ( ) and the ampicillin resistance gene (AMP) was PCR amplified from the pBluescript SK plasmid (Stratagene).

    Techniques: Reverse Transcription Polymerase Chain Reaction, Expressing, Injection