amphotericin b sodium deoxycholate  (Thermo Fisher)


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    Name:
    Gibco Amphotericin B
    Description:
    Amphotericin B is the generic version of Fungizone Fungizone is a trademark of E R Squibb Sons LLC Amphotericin B prevents the growth of fungi by causing an increase in fungal plasma membrane permeability Gibco Amphotericin B is used to prevent the contamination of cell cultures by yeast and multicellular fungi Gibco Amphotericin B contains 250 µg of amphotericin B and 205 µg of sodium deoxycholate per mL of distilled water The recommended working concentration ranges from 0 25 to 2 50 µg mL We offer a wide range of antibiotics and antimycotics in both powder and liquid formats Learn more about the use of antibiotics and antimycotics in cell culture and review guidelines for decontaminating cultures Dual site cGMP manufacturingFor supply chain continuity we manufacture Gibco Amphotericin B at two separate facilities located in Grand Island NY and Scotland UK Both sites are compliant with cGMP manufacturing requirements are certified to the ISO 13485 standard and are registered with the FDA as medical device manufacturers
    Catalog Number:
    15290018
    Price:
    None
    Category:
    Cell Culture Transfection Reagents
    Applications:
    Cell Culture|Mammalian Cell Culture
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    Structured Review

    Thermo Fisher amphotericin b sodium deoxycholate
    effect of PK11195 on intracellular Leishmania amazonensis parasites in infected macrophages. (A) Percentage of infected macrophage. Infected macrophage were treated with varying concentrations of PK11195 for 48 h in order to calculate intracellular parasites IC50/48 h. All experiments were performed in quintuplicate and independently repeated twice. (B) Intracellular parasite viability at the early stages of infection. Macrophages were infected for 6 h and then treated with PK11195 for 24 h or 48 h.(C) Intracellular parasite viability at the later stages of infection. Macrophages were infected for 96 h and then treated with PK11195 for 24 h, 48 h, or 72 h. At each treatment time point, the effect of PK11195 treatment was compared with the effect of <t>amphotericin</t> B sodium <t>deoxycholate</t> treatment. (D) Reversibility of the effect of treatment with PK11195 on the viability of intracellular Leishmania parasites. Macrophages were infected for 6 h and treated with 75 μM PK11195. After 6, 12, 24, or 48 h of exposure, macrophages were washed and incubated in PK11195-free complete medium for an additional 48 h, and then the reversibility of the effect of treatment was assessed by counting the number of viable parasites. Lines represent the median and floating bars quartiles (25% and 75%) for independent experiments performed three times in at least in triplicate (Kruskal-Wallis test, Dunn's multiple comparison test, *p
    Amphotericin B is the generic version of Fungizone Fungizone is a trademark of E R Squibb Sons LLC Amphotericin B prevents the growth of fungi by causing an increase in fungal plasma membrane permeability Gibco Amphotericin B is used to prevent the contamination of cell cultures by yeast and multicellular fungi Gibco Amphotericin B contains 250 µg of amphotericin B and 205 µg of sodium deoxycholate per mL of distilled water The recommended working concentration ranges from 0 25 to 2 50 µg mL We offer a wide range of antibiotics and antimycotics in both powder and liquid formats Learn more about the use of antibiotics and antimycotics in cell culture and review guidelines for decontaminating cultures Dual site cGMP manufacturingFor supply chain continuity we manufacture Gibco Amphotericin B at two separate facilities located in Grand Island NY and Scotland UK Both sites are compliant with cGMP manufacturing requirements are certified to the ISO 13485 standard and are registered with the FDA as medical device manufacturers
    https://www.bioz.com/result/amphotericin b sodium deoxycholate/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    amphotericin b sodium deoxycholate - by Bioz Stars, 2021-04
    99/100 stars

    Images

    1) Product Images from "In vitro evaluation of the anti-leishmanial activity and toxicity of PK11195"

    Article Title: In vitro evaluation of the anti-leishmanial activity and toxicity of PK11195

    Journal: Memórias do Instituto Oswaldo Cruz

    doi: 10.1590/0074-02760170345

    effect of PK11195 on intracellular Leishmania amazonensis parasites in infected macrophages. (A) Percentage of infected macrophage. Infected macrophage were treated with varying concentrations of PK11195 for 48 h in order to calculate intracellular parasites IC50/48 h. All experiments were performed in quintuplicate and independently repeated twice. (B) Intracellular parasite viability at the early stages of infection. Macrophages were infected for 6 h and then treated with PK11195 for 24 h or 48 h.(C) Intracellular parasite viability at the later stages of infection. Macrophages were infected for 96 h and then treated with PK11195 for 24 h, 48 h, or 72 h. At each treatment time point, the effect of PK11195 treatment was compared with the effect of amphotericin B sodium deoxycholate treatment. (D) Reversibility of the effect of treatment with PK11195 on the viability of intracellular Leishmania parasites. Macrophages were infected for 6 h and treated with 75 μM PK11195. After 6, 12, 24, or 48 h of exposure, macrophages were washed and incubated in PK11195-free complete medium for an additional 48 h, and then the reversibility of the effect of treatment was assessed by counting the number of viable parasites. Lines represent the median and floating bars quartiles (25% and 75%) for independent experiments performed three times in at least in triplicate (Kruskal-Wallis test, Dunn's multiple comparison test, *p
    Figure Legend Snippet: effect of PK11195 on intracellular Leishmania amazonensis parasites in infected macrophages. (A) Percentage of infected macrophage. Infected macrophage were treated with varying concentrations of PK11195 for 48 h in order to calculate intracellular parasites IC50/48 h. All experiments were performed in quintuplicate and independently repeated twice. (B) Intracellular parasite viability at the early stages of infection. Macrophages were infected for 6 h and then treated with PK11195 for 24 h or 48 h.(C) Intracellular parasite viability at the later stages of infection. Macrophages were infected for 96 h and then treated with PK11195 for 24 h, 48 h, or 72 h. At each treatment time point, the effect of PK11195 treatment was compared with the effect of amphotericin B sodium deoxycholate treatment. (D) Reversibility of the effect of treatment with PK11195 on the viability of intracellular Leishmania parasites. Macrophages were infected for 6 h and treated with 75 μM PK11195. After 6, 12, 24, or 48 h of exposure, macrophages were washed and incubated in PK11195-free complete medium for an additional 48 h, and then the reversibility of the effect of treatment was assessed by counting the number of viable parasites. Lines represent the median and floating bars quartiles (25% and 75%) for independent experiments performed three times in at least in triplicate (Kruskal-Wallis test, Dunn's multiple comparison test, *p

    Techniques Used: Infection, Incubation

    2) Product Images from "In vitro evaluation of the anti-leishmanial activity and toxicity of PK11195"

    Article Title: In vitro evaluation of the anti-leishmanial activity and toxicity of PK11195

    Journal: Memórias do Instituto Oswaldo Cruz

    doi: 10.1590/0074-02760170345

    effect of PK11195 on intracellular Leishmania amazonensis parasites in infected macrophages. (A) Percentage of infected macrophage. Infected macrophage were treated with varying concentrations of PK11195 for 48 h in order to calculate intracellular parasites IC50/48 h. All experiments were performed in quintuplicate and independently repeated twice. (B) Intracellular parasite viability at the early stages of infection. Macrophages were infected for 6 h and then treated with PK11195 for 24 h or 48 h.(C) Intracellular parasite viability at the later stages of infection. Macrophages were infected for 96 h and then treated with PK11195 for 24 h, 48 h, or 72 h. At each treatment time point, the effect of PK11195 treatment was compared with the effect of amphotericin B sodium deoxycholate treatment. (D) Reversibility of the effect of treatment with PK11195 on the viability of intracellular Leishmania parasites. Macrophages were infected for 6 h and treated with 75 μM PK11195. After 6, 12, 24, or 48 h of exposure, macrophages were washed and incubated in PK11195-free complete medium for an additional 48 h, and then the reversibility of the effect of treatment was assessed by counting the number of viable parasites. Lines represent the median and floating bars quartiles (25% and 75%) for independent experiments performed three times in at least in triplicate (Kruskal-Wallis test, Dunn's multiple comparison test, *p
    Figure Legend Snippet: effect of PK11195 on intracellular Leishmania amazonensis parasites in infected macrophages. (A) Percentage of infected macrophage. Infected macrophage were treated with varying concentrations of PK11195 for 48 h in order to calculate intracellular parasites IC50/48 h. All experiments were performed in quintuplicate and independently repeated twice. (B) Intracellular parasite viability at the early stages of infection. Macrophages were infected for 6 h and then treated with PK11195 for 24 h or 48 h.(C) Intracellular parasite viability at the later stages of infection. Macrophages were infected for 96 h and then treated with PK11195 for 24 h, 48 h, or 72 h. At each treatment time point, the effect of PK11195 treatment was compared with the effect of amphotericin B sodium deoxycholate treatment. (D) Reversibility of the effect of treatment with PK11195 on the viability of intracellular Leishmania parasites. Macrophages were infected for 6 h and treated with 75 μM PK11195. After 6, 12, 24, or 48 h of exposure, macrophages were washed and incubated in PK11195-free complete medium for an additional 48 h, and then the reversibility of the effect of treatment was assessed by counting the number of viable parasites. Lines represent the median and floating bars quartiles (25% and 75%) for independent experiments performed three times in at least in triplicate (Kruskal-Wallis test, Dunn's multiple comparison test, *p

    Techniques Used: Infection, Incubation

    Related Articles

    Incubation:

    Article Title: Novel and potent antimicrobial effects of caspofungin on drug-resistant Candida and bacteria
    Article Snippet: NHLF and A549 cells were incubated at 37 °C in a 90% humidified atmosphere containing 5% CO2 and subcultured every 3–5 days. .. For experiments, 5 × 103 cells/well (100 µL/well) were seeded onto 96-well flat-bottomed microplates and allowed to grow for 24 h. Caspofungin diluted with RPMI-1640, 0.9% NaCl, 5% glucose water, and dH2O, respectively, and amphotericin B (containing 5% DMSO at 500 mg/L) diluted with RPMI-1640 was applied to each well and incubated for 24 h. After incubation, wells were washed and filled with 150 µL of MTT solution (2000 mg/L MTT/PBS), and incubated for 2 h. Each well was filled with 100 µL of 100% DMSO and spectrophotometrically analyzed at 540 nm (Multiskan FC microplate photometer; Thermo Fisher Scientific). .. Cell viability was calculated as % absorbance of treated well relative to negative control well.

    MTT Assay:

    Article Title: Novel and potent antimicrobial effects of caspofungin on drug-resistant Candida and bacteria
    Article Snippet: NHLF and A549 cells were incubated at 37 °C in a 90% humidified atmosphere containing 5% CO2 and subcultured every 3–5 days. .. For experiments, 5 × 103 cells/well (100 µL/well) were seeded onto 96-well flat-bottomed microplates and allowed to grow for 24 h. Caspofungin diluted with RPMI-1640, 0.9% NaCl, 5% glucose water, and dH2O, respectively, and amphotericin B (containing 5% DMSO at 500 mg/L) diluted with RPMI-1640 was applied to each well and incubated for 24 h. After incubation, wells were washed and filled with 150 µL of MTT solution (2000 mg/L MTT/PBS), and incubated for 2 h. Each well was filled with 100 µL of 100% DMSO and spectrophotometrically analyzed at 540 nm (Multiskan FC microplate photometer; Thermo Fisher Scientific). .. Cell viability was calculated as % absorbance of treated well relative to negative control well.

    Recombinant:

    Article Title: Amphotericin B severely affects expression and activity of the endothelial constitutive nitric oxide synthase involving altered mRNA stability
    Article Snippet: With the experiments presented here we show that AmB exhibits a concentration-dependent biphasic effect on ecNOS enzyme activity which appears to be due to AmB-induced modulation of the post-transcriptional regulation of ecNOS mRNA stability, whereas promoter activity is not affected. .. Recombinant human interleukin-1β (IL-1β), recombinant murine or human tumour necrosis factor-α (TNF-α), and recombinant murine or human gamma interferon (IFN-γ) were purchased from HBT (Leiden, Netherlands) or from Genzyme (Cambridge, MA, U.S.A.), endothelial cell growth supplement (ECGS), neutral red (3% solution), type I collagen, collagenase (from Cl. histolyticum), actinomycin D, phorbol-12-myristate-13-acetate (PMA), LPS (from Salmonella typhimarium ), mouse anti α-tubulin antibody, and rabbit anti-human von Willebrand Factor (vWF) antiserum from Sigma (Deisenhofen, Germany), the monoclonal antibody Ox43 from Serotec (Camon, Wiesbaden, Germany), the monoclonal anti-ecNOS antibody from Transduction Laboratories (Lexington, KT, U.S.A.), peroxidase-conjugated porcine anti-rabbit IgG from DAKO (Hamburg, Germany), peroxidase-conjugated goat anti-mouse IgG from Zymed Laboratories, Inc. (San Francisco, CA, U.S.A.), trypsin, EDTA, RNase-free DNase I, RNase, T7 RNA polymerase, Luciferase assay system, DOTAP, foetal calf serum (FCS, endotoxin free), RPMI-1640 (endotoxin free), DNA molecular weight markers X and XIV, the oligo dT16-primer from Boehringer-Mannheim (Mannheim, Germany), Taq-polymerase, Geneticin G 418, and amphotericin B (Fungizone®) from Gibco/BRL (Eggenstein, Germany), 3,3′-diaminobenzidine (DAB) from Serva GmbH (Heidelberg, Germany), pGL3 -Basic from Promega (Madison, WI, U.S.A.), and oligonucleotides, restriction enzymes, were obtained from Pharmacia (Uppsala, Sweden). ..

    Transduction:

    Article Title: Amphotericin B severely affects expression and activity of the endothelial constitutive nitric oxide synthase involving altered mRNA stability
    Article Snippet: With the experiments presented here we show that AmB exhibits a concentration-dependent biphasic effect on ecNOS enzyme activity which appears to be due to AmB-induced modulation of the post-transcriptional regulation of ecNOS mRNA stability, whereas promoter activity is not affected. .. Recombinant human interleukin-1β (IL-1β), recombinant murine or human tumour necrosis factor-α (TNF-α), and recombinant murine or human gamma interferon (IFN-γ) were purchased from HBT (Leiden, Netherlands) or from Genzyme (Cambridge, MA, U.S.A.), endothelial cell growth supplement (ECGS), neutral red (3% solution), type I collagen, collagenase (from Cl. histolyticum), actinomycin D, phorbol-12-myristate-13-acetate (PMA), LPS (from Salmonella typhimarium ), mouse anti α-tubulin antibody, and rabbit anti-human von Willebrand Factor (vWF) antiserum from Sigma (Deisenhofen, Germany), the monoclonal antibody Ox43 from Serotec (Camon, Wiesbaden, Germany), the monoclonal anti-ecNOS antibody from Transduction Laboratories (Lexington, KT, U.S.A.), peroxidase-conjugated porcine anti-rabbit IgG from DAKO (Hamburg, Germany), peroxidase-conjugated goat anti-mouse IgG from Zymed Laboratories, Inc. (San Francisco, CA, U.S.A.), trypsin, EDTA, RNase-free DNase I, RNase, T7 RNA polymerase, Luciferase assay system, DOTAP, foetal calf serum (FCS, endotoxin free), RPMI-1640 (endotoxin free), DNA molecular weight markers X and XIV, the oligo dT16-primer from Boehringer-Mannheim (Mannheim, Germany), Taq-polymerase, Geneticin G 418, and amphotericin B (Fungizone®) from Gibco/BRL (Eggenstein, Germany), 3,3′-diaminobenzidine (DAB) from Serva GmbH (Heidelberg, Germany), pGL3 -Basic from Promega (Madison, WI, U.S.A.), and oligonucleotides, restriction enzymes, were obtained from Pharmacia (Uppsala, Sweden). ..

    Luciferase:

    Article Title: Amphotericin B severely affects expression and activity of the endothelial constitutive nitric oxide synthase involving altered mRNA stability
    Article Snippet: With the experiments presented here we show that AmB exhibits a concentration-dependent biphasic effect on ecNOS enzyme activity which appears to be due to AmB-induced modulation of the post-transcriptional regulation of ecNOS mRNA stability, whereas promoter activity is not affected. .. Recombinant human interleukin-1β (IL-1β), recombinant murine or human tumour necrosis factor-α (TNF-α), and recombinant murine or human gamma interferon (IFN-γ) were purchased from HBT (Leiden, Netherlands) or from Genzyme (Cambridge, MA, U.S.A.), endothelial cell growth supplement (ECGS), neutral red (3% solution), type I collagen, collagenase (from Cl. histolyticum), actinomycin D, phorbol-12-myristate-13-acetate (PMA), LPS (from Salmonella typhimarium ), mouse anti α-tubulin antibody, and rabbit anti-human von Willebrand Factor (vWF) antiserum from Sigma (Deisenhofen, Germany), the monoclonal antibody Ox43 from Serotec (Camon, Wiesbaden, Germany), the monoclonal anti-ecNOS antibody from Transduction Laboratories (Lexington, KT, U.S.A.), peroxidase-conjugated porcine anti-rabbit IgG from DAKO (Hamburg, Germany), peroxidase-conjugated goat anti-mouse IgG from Zymed Laboratories, Inc. (San Francisco, CA, U.S.A.), trypsin, EDTA, RNase-free DNase I, RNase, T7 RNA polymerase, Luciferase assay system, DOTAP, foetal calf serum (FCS, endotoxin free), RPMI-1640 (endotoxin free), DNA molecular weight markers X and XIV, the oligo dT16-primer from Boehringer-Mannheim (Mannheim, Germany), Taq-polymerase, Geneticin G 418, and amphotericin B (Fungizone®) from Gibco/BRL (Eggenstein, Germany), 3,3′-diaminobenzidine (DAB) from Serva GmbH (Heidelberg, Germany), pGL3 -Basic from Promega (Madison, WI, U.S.A.), and oligonucleotides, restriction enzymes, were obtained from Pharmacia (Uppsala, Sweden). ..

    Molecular Weight:

    Article Title: Amphotericin B severely affects expression and activity of the endothelial constitutive nitric oxide synthase involving altered mRNA stability
    Article Snippet: With the experiments presented here we show that AmB exhibits a concentration-dependent biphasic effect on ecNOS enzyme activity which appears to be due to AmB-induced modulation of the post-transcriptional regulation of ecNOS mRNA stability, whereas promoter activity is not affected. .. Recombinant human interleukin-1β (IL-1β), recombinant murine or human tumour necrosis factor-α (TNF-α), and recombinant murine or human gamma interferon (IFN-γ) were purchased from HBT (Leiden, Netherlands) or from Genzyme (Cambridge, MA, U.S.A.), endothelial cell growth supplement (ECGS), neutral red (3% solution), type I collagen, collagenase (from Cl. histolyticum), actinomycin D, phorbol-12-myristate-13-acetate (PMA), LPS (from Salmonella typhimarium ), mouse anti α-tubulin antibody, and rabbit anti-human von Willebrand Factor (vWF) antiserum from Sigma (Deisenhofen, Germany), the monoclonal antibody Ox43 from Serotec (Camon, Wiesbaden, Germany), the monoclonal anti-ecNOS antibody from Transduction Laboratories (Lexington, KT, U.S.A.), peroxidase-conjugated porcine anti-rabbit IgG from DAKO (Hamburg, Germany), peroxidase-conjugated goat anti-mouse IgG from Zymed Laboratories, Inc. (San Francisco, CA, U.S.A.), trypsin, EDTA, RNase-free DNase I, RNase, T7 RNA polymerase, Luciferase assay system, DOTAP, foetal calf serum (FCS, endotoxin free), RPMI-1640 (endotoxin free), DNA molecular weight markers X and XIV, the oligo dT16-primer from Boehringer-Mannheim (Mannheim, Germany), Taq-polymerase, Geneticin G 418, and amphotericin B (Fungizone®) from Gibco/BRL (Eggenstein, Germany), 3,3′-diaminobenzidine (DAB) from Serva GmbH (Heidelberg, Germany), pGL3 -Basic from Promega (Madison, WI, U.S.A.), and oligonucleotides, restriction enzymes, were obtained from Pharmacia (Uppsala, Sweden). ..

    Fluorescence:

    Article Title: Transcriptome Sequencing and Comparative Analysis of Amphoteric ESCs and PGCs in Chicken (Gallus gallus)
    Article Snippet: The induction medium was Dulbecco’s Modified Eagle Medium (DMEM) supplemented with10 mM RA (Solarbio, Beijing, China, IR0060) and 15% Fetal Bovine Serum (FBS) (Gibco, New York, NY, USA, 26140). .. Fluorescence Activated Cell Sorter (FACS)Cells harvested from amphoteric ESCs and PGCs were blocked with blocking buffer (Phosphate Buffered Saline containing 10% fetal bovine serum) (Gibco, New York, NY, USA, 10270-106) for 2 h at 37 °C. .. Samples were incubated with antibodies against cell surface epitopes (SSEA-1, abcam, Cambridge, UK, ab16285,1:100; SOX2, abcam, Cambridge, UK, ab93689,1:100; CKIT, Thermo Fisher Scientific, Shanghai, China, 14-1172-81,1:100) at 4 °C overnight and washed with PBS containing 0.1% Tween-20 (Solarbio, Beijing, China, T8220) three times, followed by fluorescence coupled secondary antibody (Goat Anti-Rabbit IgG FITC Conjugated, CWBIO, Shanghai, China, CW0114S, 1:100; Goat Anti-Mouse IgG H & L (TRICT), abcam, Cambridge, UK, ab6786, 1:100) incubation at 37 °C for 2 h. Then, the cells were washed with PBS containing 0.1% Tween-20 three times.

    FACS:

    Article Title: Transcriptome Sequencing and Comparative Analysis of Amphoteric ESCs and PGCs in Chicken (Gallus gallus)
    Article Snippet: The induction medium was Dulbecco’s Modified Eagle Medium (DMEM) supplemented with10 mM RA (Solarbio, Beijing, China, IR0060) and 15% Fetal Bovine Serum (FBS) (Gibco, New York, NY, USA, 26140). .. Fluorescence Activated Cell Sorter (FACS)Cells harvested from amphoteric ESCs and PGCs were blocked with blocking buffer (Phosphate Buffered Saline containing 10% fetal bovine serum) (Gibco, New York, NY, USA, 10270-106) for 2 h at 37 °C. .. Samples were incubated with antibodies against cell surface epitopes (SSEA-1, abcam, Cambridge, UK, ab16285,1:100; SOX2, abcam, Cambridge, UK, ab93689,1:100; CKIT, Thermo Fisher Scientific, Shanghai, China, 14-1172-81,1:100) at 4 °C overnight and washed with PBS containing 0.1% Tween-20 (Solarbio, Beijing, China, T8220) three times, followed by fluorescence coupled secondary antibody (Goat Anti-Rabbit IgG FITC Conjugated, CWBIO, Shanghai, China, CW0114S, 1:100; Goat Anti-Mouse IgG H & L (TRICT), abcam, Cambridge, UK, ab6786, 1:100) incubation at 37 °C for 2 h. Then, the cells were washed with PBS containing 0.1% Tween-20 three times.

    Blocking Assay:

    Article Title: Transcriptome Sequencing and Comparative Analysis of Amphoteric ESCs and PGCs in Chicken (Gallus gallus)
    Article Snippet: The induction medium was Dulbecco’s Modified Eagle Medium (DMEM) supplemented with10 mM RA (Solarbio, Beijing, China, IR0060) and 15% Fetal Bovine Serum (FBS) (Gibco, New York, NY, USA, 26140). .. Fluorescence Activated Cell Sorter (FACS)Cells harvested from amphoteric ESCs and PGCs were blocked with blocking buffer (Phosphate Buffered Saline containing 10% fetal bovine serum) (Gibco, New York, NY, USA, 10270-106) for 2 h at 37 °C. .. Samples were incubated with antibodies against cell surface epitopes (SSEA-1, abcam, Cambridge, UK, ab16285,1:100; SOX2, abcam, Cambridge, UK, ab93689,1:100; CKIT, Thermo Fisher Scientific, Shanghai, China, 14-1172-81,1:100) at 4 °C overnight and washed with PBS containing 0.1% Tween-20 (Solarbio, Beijing, China, T8220) three times, followed by fluorescence coupled secondary antibody (Goat Anti-Rabbit IgG FITC Conjugated, CWBIO, Shanghai, China, CW0114S, 1:100; Goat Anti-Mouse IgG H & L (TRICT), abcam, Cambridge, UK, ab6786, 1:100) incubation at 37 °C for 2 h. Then, the cells were washed with PBS containing 0.1% Tween-20 three times.

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    Thermo Fisher amphotericin b sodium deoxycholate
    effect of PK11195 on intracellular Leishmania amazonensis parasites in infected macrophages. (A) Percentage of infected macrophage. Infected macrophage were treated with varying concentrations of PK11195 for 48 h in order to calculate intracellular parasites IC50/48 h. All experiments were performed in quintuplicate and independently repeated twice. (B) Intracellular parasite viability at the early stages of infection. Macrophages were infected for 6 h and then treated with PK11195 for 24 h or 48 h.(C) Intracellular parasite viability at the later stages of infection. Macrophages were infected for 96 h and then treated with PK11195 for 24 h, 48 h, or 72 h. At each treatment time point, the effect of PK11195 treatment was compared with the effect of <t>amphotericin</t> B sodium <t>deoxycholate</t> treatment. (D) Reversibility of the effect of treatment with PK11195 on the viability of intracellular Leishmania parasites. Macrophages were infected for 6 h and treated with 75 μM PK11195. After 6, 12, 24, or 48 h of exposure, macrophages were washed and incubated in PK11195-free complete medium for an additional 48 h, and then the reversibility of the effect of treatment was assessed by counting the number of viable parasites. Lines represent the median and floating bars quartiles (25% and 75%) for independent experiments performed three times in at least in triplicate (Kruskal-Wallis test, Dunn's multiple comparison test, *p
    Amphotericin B Sodium Deoxycholate, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/amphotericin b sodium deoxycholate/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    amphotericin b sodium deoxycholate - by Bioz Stars, 2021-04
    99/100 stars
      Buy from Supplier

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    effect of PK11195 on intracellular Leishmania amazonensis parasites in infected macrophages. (A) Percentage of infected macrophage. Infected macrophage were treated with varying concentrations of PK11195 for 48 h in order to calculate intracellular parasites IC50/48 h. All experiments were performed in quintuplicate and independently repeated twice. (B) Intracellular parasite viability at the early stages of infection. Macrophages were infected for 6 h and then treated with PK11195 for 24 h or 48 h.(C) Intracellular parasite viability at the later stages of infection. Macrophages were infected for 96 h and then treated with PK11195 for 24 h, 48 h, or 72 h. At each treatment time point, the effect of PK11195 treatment was compared with the effect of amphotericin B sodium deoxycholate treatment. (D) Reversibility of the effect of treatment with PK11195 on the viability of intracellular Leishmania parasites. Macrophages were infected for 6 h and treated with 75 μM PK11195. After 6, 12, 24, or 48 h of exposure, macrophages were washed and incubated in PK11195-free complete medium for an additional 48 h, and then the reversibility of the effect of treatment was assessed by counting the number of viable parasites. Lines represent the median and floating bars quartiles (25% and 75%) for independent experiments performed three times in at least in triplicate (Kruskal-Wallis test, Dunn's multiple comparison test, *p

    Journal: Memórias do Instituto Oswaldo Cruz

    Article Title: In vitro evaluation of the anti-leishmanial activity and toxicity of PK11195

    doi: 10.1590/0074-02760170345

    Figure Lengend Snippet: effect of PK11195 on intracellular Leishmania amazonensis parasites in infected macrophages. (A) Percentage of infected macrophage. Infected macrophage were treated with varying concentrations of PK11195 for 48 h in order to calculate intracellular parasites IC50/48 h. All experiments were performed in quintuplicate and independently repeated twice. (B) Intracellular parasite viability at the early stages of infection. Macrophages were infected for 6 h and then treated with PK11195 for 24 h or 48 h.(C) Intracellular parasite viability at the later stages of infection. Macrophages were infected for 96 h and then treated with PK11195 for 24 h, 48 h, or 72 h. At each treatment time point, the effect of PK11195 treatment was compared with the effect of amphotericin B sodium deoxycholate treatment. (D) Reversibility of the effect of treatment with PK11195 on the viability of intracellular Leishmania parasites. Macrophages were infected for 6 h and treated with 75 μM PK11195. After 6, 12, 24, or 48 h of exposure, macrophages were washed and incubated in PK11195-free complete medium for an additional 48 h, and then the reversibility of the effect of treatment was assessed by counting the number of viable parasites. Lines represent the median and floating bars quartiles (25% and 75%) for independent experiments performed three times in at least in triplicate (Kruskal-Wallis test, Dunn's multiple comparison test, *p

    Article Snippet: Amphotericin B sodium deoxycholate (Fungizone, Gibco) was purchased from Life Technologies (Carlsbad, CA, USA) as a ready-to-use solution (271 µM).

    Techniques: Infection, Incubation

    effect of PK11195 on intracellular Leishmania amazonensis parasites in infected macrophages. (A) Percentage of infected macrophage. Infected macrophage were treated with varying concentrations of PK11195 for 48 h in order to calculate intracellular parasites IC50/48 h. All experiments were performed in quintuplicate and independently repeated twice. (B) Intracellular parasite viability at the early stages of infection. Macrophages were infected for 6 h and then treated with PK11195 for 24 h or 48 h.(C) Intracellular parasite viability at the later stages of infection. Macrophages were infected for 96 h and then treated with PK11195 for 24 h, 48 h, or 72 h. At each treatment time point, the effect of PK11195 treatment was compared with the effect of amphotericin B sodium deoxycholate treatment. (D) Reversibility of the effect of treatment with PK11195 on the viability of intracellular Leishmania parasites. Macrophages were infected for 6 h and treated with 75 μM PK11195. After 6, 12, 24, or 48 h of exposure, macrophages were washed and incubated in PK11195-free complete medium for an additional 48 h, and then the reversibility of the effect of treatment was assessed by counting the number of viable parasites. Lines represent the median and floating bars quartiles (25% and 75%) for independent experiments performed three times in at least in triplicate (Kruskal-Wallis test, Dunn's multiple comparison test, *p

    Journal: Memórias do Instituto Oswaldo Cruz

    Article Title: In vitro evaluation of the anti-leishmanial activity and toxicity of PK11195

    doi: 10.1590/0074-02760170345

    Figure Lengend Snippet: effect of PK11195 on intracellular Leishmania amazonensis parasites in infected macrophages. (A) Percentage of infected macrophage. Infected macrophage were treated with varying concentrations of PK11195 for 48 h in order to calculate intracellular parasites IC50/48 h. All experiments were performed in quintuplicate and independently repeated twice. (B) Intracellular parasite viability at the early stages of infection. Macrophages were infected for 6 h and then treated with PK11195 for 24 h or 48 h.(C) Intracellular parasite viability at the later stages of infection. Macrophages were infected for 96 h and then treated with PK11195 for 24 h, 48 h, or 72 h. At each treatment time point, the effect of PK11195 treatment was compared with the effect of amphotericin B sodium deoxycholate treatment. (D) Reversibility of the effect of treatment with PK11195 on the viability of intracellular Leishmania parasites. Macrophages were infected for 6 h and treated with 75 μM PK11195. After 6, 12, 24, or 48 h of exposure, macrophages were washed and incubated in PK11195-free complete medium for an additional 48 h, and then the reversibility of the effect of treatment was assessed by counting the number of viable parasites. Lines represent the median and floating bars quartiles (25% and 75%) for independent experiments performed three times in at least in triplicate (Kruskal-Wallis test, Dunn's multiple comparison test, *p

    Article Snippet: Amphotericin B sodium deoxycholate (Fungizone, Gibco) was purchased from Life Technologies (Carlsbad, CA, USA) as a ready-to-use solution (271 µM).

    Techniques: Infection, Incubation